Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0012094

Physical Location & Seq

Genomic Coordinates Forward (+)

19057991 .. 19062342

Gene Structure 4352 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1103000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGGAAGAAGGCACTTCATTTTTGTTCTCCGGCGTCAACTTCAACCGGAAAAAGTTTGCCACGGACTTCTCCAGATTCCAGAACAAAGAGAGCGAGAAAGTGGTAGAAGATTCGAGCTTGTTGGAAATTCCAAAATCTGAGGCGGAGGTGGAGGCAGAGAAGGAGGAGCCAGTAGTGCCGGTTAAGAAGAGGAAGCGGAAGGGGGAGGTTTCCGAGGGTGTGGAGGGGATTAGTGTCTTTAAGACCTCAAAATCGGCGAAGGCAGCGAAAGCGGGGAAAGAGAAGCCTGGCAATGAAATCACGGAGCAGAAGAAGAAGTTGAATCGGCAACTCGAGCGGGATTCGCTGACGCGAAAGAAGTATAACATTCATGTGTCCGGGAATAACATCCCTTCACCACTTGATAATTTTGCAGACTTATCCTCAAGATATGGATGCGAGCCATATTTACTTCAGAATTTGGCAGAACTTGGATTTAAAGAACCAACGCCAATCCAAAGGCAGGCTATTCCAGTCCTCTTATCTGGTCGAGAATGCTTTGCTTGTGCTCCAACTGGATCTGGAAAAACTCTTGCTTTTGTTTGTCCCATGCTAATGAAACTTAAGCATACATCTAAGGATGGTATCCGAGCTGTTATTCTTTGTCCAACACGTGAGTTAGCTGCTCAGACAACCAGAGAGTGCAAAAAGATGGCAAAAGGAAATAAATTCTACATCAAGTTAATGACCAAAGAGCTTGCAAGAAATGCCGATTTTTCAAAAATACGCTGTGATATACTCATTTCTACTCCACTTCGGTTACGGTTGGCTATCCGGAAAAAGAAGGTTGATTTAAGCAGGGTTGAGTATCTGGTATTGGATGAGTCTGATAAGCTTTTTGAGCTTGGCTTGTTAAAGCAGATTGATTCTGTGGTCAAAGCATGTTCGAACCCTTCAATTATTCGCTCCTTATTTAGTGCTACTTTACCTGATTTCGTTGAGGAGCTTGCCCGCACCATAATGCATGATGCTGTTCGAGTTATCATTGGCAGGAAAAATACTGCTTCTGACACTATCAAGCAAAAACTGGTCTTTGCTGGAAGTGAAGAAGGGAAGCTATTAGCACTTCGTCAAAGCTTTAAGGAGAGTCTGAATCCACCAGTGCTGATATTTTTACAAAGCAAGGAGCGAGCTAAGGAACTCTACGGAGAACTGTTGTTTGAGAATGTAAGAGTTGGTGCCATACATTCTGATCTGTCACAAACACAGCGAGAAAATGCTGTAGATGACTTCAGAGCTGGAAAAACATGGGTTTTAATTGCCACTGATGTTATTGCTCGGGGTATGGATTTCAAAGGTGTCAACTGTGTAATTAATTATGATTTTCCAGATTCTGCGGCTGCGTACATTCACAGGATTGGTCGGTGTGGACGAGCAGGGAGGAGCGGAGAAGCTATTACTTTCTACACAGAGGCAGATATTCCATATCTACGGAATATAGCTAATGTGATGTCAACATCAGGTTGTGAAGTTCCGTCTTGGATCATGGCCTTGCGCAAACAGAAGTGGAAAAAACACCGACCCGGAAGAGAATCGATATCAACCCAACCGAAAGATGAGAAGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0010501 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031974 GO:0031981 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

536

Amino Acids

60.06

Weight (kDa)

9.41

Isoelectric Point (pI)

40.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	163 - 328	1.1e-39	DEAD/DEAH box helicase
ResIII	PF04851	163 - 321	8.9e-09	Type III restriction enzyme, res subunit
Helicase_C	PF00271	365 - 473	6.8e-31	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012094)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09720 AT3G09720
fragaria_vesca	FvH4_6g15470
malus_domestica	MD04G1103000.v1.1 MD12G1123200.v1.1
prunus_persica	Prupe.6G240000_v2.0.a1 Prupe.6G240000_v2.0.a1
pyrus_communis	pycom04g09810 pycom12g12190
rosa_chinensis	RchiOBHm_Chr3g0468951
rosa_laevigata	RLG00000024397
rosa_multiflora	Rmu_co8069278.1_g000001 Rmu_co8291353.1_g000001
rosa_roxburghii	Rroxscaffold_6G00412430
rosa_rugosa	Rorug03G0097800
rosa_samantha	Rh3AG147400 Rh3BG170000 Rh3DG205000
rosa_wichuraiana	Rw3G013880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1535
AccB1I	GGYRCC	1 cut(s)	1217
AccBSI	CCGCTC	2 cut(s)	337, 1425
AccII	CGCG	1 cut(s)	352
AccIII	TCCGGA	1 cut(s)	813
AciI	CCGC	7 cut(s)	143, 196, 272, 337, 991, 1376, 1425
AclWI	GGATC	2 cut(s)	565, 1529
AcsI	RAATTY	3 cut(s)	126, 457, 707
AcuI	CTGAAG	2 cut(s)	437, 1255
AcvI	CACGTG	1 cut(s)	653
AcyI	GRCGYC	1 cut(s)	33
AfaI	GTAC	1 cut(s)	1385
AfiI	CCNNNNNNNGG	1 cut(s)	502
AflII	CTTAAG	1 cut(s)	602
AflIII	ACRYGT	1 cut(s)	650
AgsI	TTSAA	5 cut(s)	43, 322, 759, 936, 1333
AjnI	CCWGG	1 cut(s)	286
Alw21I	GWGCWC	1 cut(s)	550
AlwI	GGATC	2 cut(s)	565, 1529
AlwNI	CAGNNNCTG	1 cut(s)	1373
Ama87I	CYCGRG	2 cut(s)	332, 1317
Aor13HI	TCCGGA	1 cut(s)	813
AoxI	GGCC	1 cut(s)	1527
ApeKI	GCWGC	3 cut(s)	263, 662, 1379
ApoI	RAATTY	3 cut(s)	126, 457, 707
AseI	ATTAAT	1 cut(s)	1353
AspLEI	GCGC	1 cut(s)	1536
AsuC2I	CCSGG	2 cut(s)	379, 1563
AsuHPI	GGTGA	1 cut(s)	387
AsuII	TTCGAA	1 cut(s)	926
AvaI	CYCGRG	2 cut(s)	332, 1317
BanI	GGYRCC	1 cut(s)	1217
BbrPI	CACGTG	1 cut(s)	653
Bbv12I	GWGCWC	1 cut(s)	550
BbvI	GCAGC	3 cut(s)	275, 649, 1366
BccI	CCATC	2 cut(s)	614, 685
BciT130I	CCWGG	1 cut(s)	288
BciVI	GTATCC	1 cut(s)	635
BcnI	CCSGG	2 cut(s)	379, 1563
BfmI	CTRYAG	1 cut(s)	1260
BfrI	CTTAAG	1 cut(s)	602
BfuI	GTATCC	1 cut(s)	635
BisI	GCNGC	4 cut(s)	264, 663, 1377, 1380
BlsI	GCNGC	4 cut(s)	265, 664, 1378, 1381
Bme1390I	CCNGG	3 cut(s)	288, 379, 1563
BmeT110I	CYCGRG	2 cut(s)	332, 1317
BmiI	GGNNCC	2 cut(s)	168, 1219
BmrFI	CCNGG	3 cut(s)	288, 379, 1563
BmsI	GCATC	2 cut(s)	425, 997
BpmI	CTGGAG	1 cut(s)	55
Bpu10I	CCTNAGC	1 cut(s)	1173
Bpu14I	TTCGAA	1 cut(s)	926
BpuEI	CTTGAG	1 cut(s)	409
BpuMI	CCSGG	2 cut(s)	379, 1563
Bsa29I	ATCGAT	1 cut(s)	1574
BsaAI	YACGTR	1 cut(s)	653
BsaHI	GRCGYC	1 cut(s)	33
BsaJI	CCNNGG	2 cut(s)	60, 213
BsaWI	WCCGGW	2 cut(s)	45, 813
Bsc4I	CCNNNNNNNGG	1 cut(s)	502
Bse118I	RCCGGY	1 cut(s)	178
Bse1I	ACTGG	5 cut(s)	170, 512, 559, 1071, 1139
Bse3DI	GCAATG	1 cut(s)	298
BseAI	TCCGGA	1 cut(s)	813
BseBI	CCWGG	1 cut(s)	288
BseCI	ATCGAT	1 cut(s)	1574
BseDI	CCNNGG	2 cut(s)	60, 213
BseGI	GGATG	4 cut(s)	387, 440, 625, 865
BseLI	CCNNNNNNNGG	1 cut(s)	502
BseMI	GCAATG	1 cut(s)	298
BseMII	CTCAG	2 cut(s)	129, 680
BseNI	ACTGG	5 cut(s)	170, 512, 559, 1071, 1139
BseRI	GAGGAG	3 cut(s)	179, 995, 1435
BseXI	GCAGC	3 cut(s)	275, 649, 1366
Bsh1236I	CGCG	1 cut(s)	352
BshFI	GGCC	1 cut(s)	1529
BshNI	GGYRCC	1 cut(s)	1217
BshVI	ATCGAT	1 cut(s)	1574
BsiHKAI	GWGCWC	1 cut(s)	550
BsiHKCI	CYCGRG	2 cut(s)	332, 1317
BsiSI	CCGG	6 cut(s)	30, 46, 179, 378, 814, 1563
BslFI	GGGAC	1 cut(s)	570
BslI	CCNNNNNNNGG	1 cut(s)	502
BsmFI	GGGAC	1 cut(s)	570
BsmI	GAATGC	1 cut(s)	539
BsnI	GGCC	1 cut(s)	1529
BsoBI	CYCGRG	2 cut(s)	332, 1317
Bsp119I	TTCGAA	1 cut(s)	926
Bsp1286I	GDGCHC	1 cut(s)	550
Bsp13I	TCCGGA	1 cut(s)	813
Bsp143I	GATC	3 cut(s)	557, 1231, 1521
BspACI	CCGC	7 cut(s)	143, 196, 272, 337, 991, 1376, 1425
BspANI	GGCC	1 cut(s)	1529
BspCNI	CTCAG	2 cut(s)	130, 679
BspDI	ATCGAT	1 cut(s)	1574
BspEI	TCCGGA	1 cut(s)	813
BspFNI	CGCG	1 cut(s)	352
BspLI	GGNNCC	2 cut(s)	168, 1219
BspPI	GGATC	2 cut(s)	565, 1529
BspT104I	TTCGAA	1 cut(s)	926
BspT107I	GGYRCC	1 cut(s)	1217
BspTI	CTTAAG	1 cut(s)	602
BsrBI	CCGCTC	2 cut(s)	337, 1425
BsrDI	GCAATG	1 cut(s)	298
BsrFI	RCCGGY	1 cut(s)	178
BsrI	ACTGG	5 cut(s)	170, 512, 559, 1071, 1139
BssAI	RCCGGY	1 cut(s)	178
BssECI	CCNNGG	2 cut(s)	60, 213
BssMI	GATC	3 cut(s)	557, 1231, 1521
BssNI	GRCGYC	1 cut(s)	33
Bst2UI	CCWGG	1 cut(s)	288
Bst4CI	ACNGT	3 cut(s)	804, 1194, 1346
Bst6I	CTCTTC	2 cut(s)	182, 1561
BstACI	GRCGYC	1 cut(s)	33
BstAFI	CTTAAG	1 cut(s)	602
BstAPI	GCANNNNNTGC	1 cut(s)	746
BstBAI	YACGTR	1 cut(s)	653
BstBI	TTCGAA	1 cut(s)	926
BstC8I	GCNNGC	6 cut(s)	440, 504, 738, 987, 991, 1170
BstDEI	CTNAG	4 cut(s)	138, 615, 666, 1173
BstDSI	CCRYGG	1 cut(s)	60
BstF5I	GGATG	4 cut(s)	387, 440, 625, 865
BstFNI	CGCG	1 cut(s)	352
BstHHI	GCGC	1 cut(s)	1536
BstKTI	GATC	3 cut(s)	560, 1234, 1524
BstMBI	GATC	3 cut(s)	557, 1231, 1521
BstMWI	GCNNNNNNNGC	7 cut(s)	175, 263, 269, 334, 343, 746, 880
BstNI	CCWGG	1 cut(s)	288
BstNSI	RCATGY	1 cut(s)	924
BstSCI	CCNGG	3 cut(s)	286, 377, 1561
BstSFI	CTRYAG	1 cut(s)	1260
BstUI	CGCG	1 cut(s)	352
BstV1I	GCAGC	3 cut(s)	275, 649, 1366
BstX2I	RGATCY	1 cut(s)	557
BstYI	RGATCY	1 cut(s)	557
Bsu15I	ATCGAT	1 cut(s)	1574
BsuI	GTATCC	1 cut(s)	635
BsuRI	GGCC	1 cut(s)	1529
BsuTUI	ATCGAT	1 cut(s)	1574
BtgI	CCRYGG	1 cut(s)	60
BtsCI	GGATG	4 cut(s)	387, 440, 625, 865
BtsIMutI	CAGTG	2 cut(s)	1146, 1302
Cac8I	GCNNGC	6 cut(s)	440, 504, 738, 987, 991, 1170
CaiI	CAGNNNCTG	1 cut(s)	1373
CfoI	GCGC	1 cut(s)	1536
Cfr10I	RCCGGY	1 cut(s)	178
ClaI	ATCGAT	1 cut(s)	1574
CseI	GACGC	2 cut(s)	22, 358
Csp6I	GTAC	1 cut(s)	1384
CviAII	CATG	6 cut(s)	371, 589, 921, 1004, 1287, 1525
CviQI	GTAC	1 cut(s)	1384
DdeI	CTNAG	4 cut(s)	138, 615, 666, 1173
DpnI	GATC	3 cut(s)	559, 1233, 1523
DpnII	GATC	3 cut(s)	557, 1231, 1521
DraI	TTTAAA	1 cut(s)	478
Eam1104I	CTCTTC	2 cut(s)	182, 1561
EarI	CTCTTC	2 cut(s)	182, 1561
EciI	GGCGGA	1 cut(s)	158
Eco32I	GATATC	1 cut(s)	1578
Eco57I	CTGAAG	2 cut(s)	437, 1255
Eco72I	CACGTG	1 cut(s)	653
Eco88I	CYCGRG	2 cut(s)	332, 1317
EcoRII	CCWGG	1 cut(s)	286
EcoRV	GATATC	1 cut(s)	1578
EcoT22I	ATGCAT	1 cut(s)	1005
FaeI	CATG	6 cut(s)	374, 592, 924, 1007, 1290, 1528
FaqI	GGGAC	1 cut(s)	570
FatI	CATG	6 cut(s)	370, 588, 920, 1003, 1286, 1524
FauI	CCCGC	3 cut(s)	265, 330, 998
Fnu4HI	GCNGC	4 cut(s)	264, 663, 1377, 1380
FokI	GGATG	4 cut(s)	374, 447, 632, 872
Fsp4HI	GCNGC	4 cut(s)	264, 663, 1377, 1380
FspI	TGCGCA	1 cut(s)	1535
GlaI	GCGC	1 cut(s)	1535
GluI	GCNGC	4 cut(s)	264, 663, 1377, 1380
GsuI	CTGGAG	1 cut(s)	55
HaeIII	GGCC	1 cut(s)	1529
HapII	CCGG	6 cut(s)	30, 46, 179, 378, 814, 1563
HgaI	GACGC	2 cut(s)	22, 358
HhaI	GCGC	1 cut(s)	1536
Hin1I	GRCGYC	1 cut(s)	33
Hin1II	CATG	6 cut(s)	374, 592, 924, 1007, 1290, 1528
Hin6I	GCGC	1 cut(s)	1534
HinP1I	GCGC	1 cut(s)	1534
HincII	GTYRAC	3 cut(s)	37, 1342, 1494
HindII	GTYRAC	3 cut(s)	37, 1342, 1494
HindIII	AAGCTT	2 cut(s)	872, 1114
HpaII	CCGG	6 cut(s)	30, 46, 179, 378, 814, 1563
HphI	GGTGA	1 cut(s)	387
Hpy166II	GTNNAC	4 cut(s)	37, 1342, 1409, 1494
Hpy188III	TCNNGA	7 cut(s)	72, 79, 426, 530, 561, 814, 1367
Hpy8I	GTNNAC	4 cut(s)	37, 1342, 1409, 1494
HpyAV	CCTTC	8 cut(s)	154, 193, 253, 402, 817, 942, 1082, 1594
HpyCH4III	ACNGT	3 cut(s)	804, 1194, 1346
HpyCH4IV	ACGT	1 cut(s)	652
HpyCH4V	TGCA	4 cut(s)	413, 684, 740, 1003
HpyF10VI	GCNNNNNNNGC	7 cut(s)	175, 263, 269, 334, 343, 746, 880
HpyF3I	CTNAG	4 cut(s)	138, 615, 666, 1173
HpySE526I	ACGT	1 cut(s)	652
Hsp92I	GRCGYC	1 cut(s)	33
Hsp92II	CATG	6 cut(s)	374, 592, 924, 1007, 1290, 1528
HspAI	GCGC	1 cut(s)	1534
Kpn2I	TCCGGA	1 cut(s)	813
Kzo9I	GATC	3 cut(s)	557, 1231, 1521
LmnI	GCTCC	7 cut(s)	166, 304, 553, 950, 982, 1165, 1422
Lsp1109I	GCAGC	3 cut(s)	275, 649, 1366
LweI	GCATC	2 cut(s)	425, 997
MaeII	ACGT	1 cut(s)	652
MaeIII	GTNAC	2 cut(s)	798, 1236
MalI	GATC	3 cut(s)	559, 1233, 1523
MbiI	CCGCTC	2 cut(s)	337, 1425
MboI	GATC	3 cut(s)	557, 1231, 1521
MboII	GAAGA	7 cut(s)	17, 119, 199, 322, 325, 1097, 1578
MflI	RGATCY	1 cut(s)	557
MhlI	GDGCHC	1 cut(s)	550
MluCI	AATT	8 cut(s)	126, 406, 457, 707, 936, 1296, 1350, 1354
MlyI	GAGTC	2 cut(s)	872, 1135
MmeI	TCCRAC	3 cut(s)	102, 575, 671
Mph1103I	ATGCAT	1 cut(s)	1005
MroI	TCCGGA	1 cut(s)	813
MslI	CAYNNNNRTG	3 cut(s)	371, 593, 998
MspCI	CTTAAG	1 cut(s)	602
MspI	CCGG	6 cut(s)	30, 46, 179, 378, 814, 1563
MspR9I	CCNGG	3 cut(s)	288, 379, 1563
Mva1269I	GAATGC	1 cut(s)	539
MvaI	CCWGG	1 cut(s)	288
MvnI	CGCG	1 cut(s)	352
MwoI	GCNNNNNNNGC	7 cut(s)	175, 263, 269, 334, 343, 746, 880
NciI	CCSGG	2 cut(s)	379, 1563
NdeII	GATC	3 cut(s)	557, 1231, 1521
NlaIII	CATG	6 cut(s)	374, 592, 924, 1007, 1290, 1528
NlaIV	GGNNCC	2 cut(s)	168, 1219
NmuCI	GTSAC	1 cut(s)	1236
NsbI	TGCGCA	1 cut(s)	1535
NsiI	ATGCAT	1 cut(s)	1005
NspI	RCATGY	1 cut(s)	924
NspV	TTCGAA	1 cut(s)	926
PaeR7I	CTCGAG	1 cut(s)	332
PcsI	WCGNNNNNNNCGW	1 cut(s)	1408
PctI	GAATGC	1 cut(s)	539
PfeI	GAWTC	8 cut(s)	75, 110, 322, 341, 905, 1132, 1370, 1571
PfoI	TCCNGGA	1 cut(s)	377
PkrI	GCNGC	4 cut(s)	265, 664, 1378, 1381
PleI	GAGTC	2 cut(s)	871, 1134
PmaCI	CACGTG	1 cut(s)	653
PmlI	CACGTG	1 cut(s)	653
PpsI	GAGTC	2 cut(s)	871, 1134
Ppu21I	YACGTR	1 cut(s)	653
PshBI	ATTAAT	1 cut(s)	1353
Psp6I	CCWGG	1 cut(s)	286
PspCI	CACGTG	1 cut(s)	653
PspGI	CCWGG	1 cut(s)	286
PspN4I	GGNNCC	2 cut(s)	168, 1219
PspXI	VCTCGAGB	1 cut(s)	332
PstNI	CAGNNNCTG	1 cut(s)	1373
PsuI	RGATCY	1 cut(s)	557
RsaI	GTAC	1 cut(s)	1385
RsaNI	GTAC	1 cut(s)	1384
RseI	CAYNNNNRTG	3 cut(s)	371, 593, 998
SatI	GCNGC	4 cut(s)	264, 663, 1377, 1380
Sau3AI	GATC	3 cut(s)	557, 1231, 1521
SchI	GAGTC	2 cut(s)	872, 1135
ScrFI	CCNGG	3 cut(s)	288, 379, 1563
SduI	GDGCHC	1 cut(s)	550
SfaNI	GCATC	2 cut(s)	425, 997
SfcI	CTRYAG	1 cut(s)	1260
Sfr274I	CTCGAG	1 cut(s)	332
SfuI	TTCGAA	1 cut(s)	926
SlaI	CTCGAG	1 cut(s)	332
SmiMI	CAYNNNNRTG	3 cut(s)	371, 593, 998
SmlI	CTYRAG	3 cut(s)	332, 424, 602
SmoI	CTYRAG	3 cut(s)	332, 424, 602
Sse9I	AATT	8 cut(s)	126, 406, 457, 707, 936, 1296, 1350, 1354
SsiI	CCGC	7 cut(s)	143, 196, 272, 337, 991, 1376, 1425
StyD4I	CCNGG	3 cut(s)	286, 377, 1561
TaaI	ACNGT	3 cut(s)	804, 1194, 1346
TaiI	ACGT	1 cut(s)	655
TaqI	TCGA	6 cut(s)	113, 333, 529, 926, 1015, 1574
TasI	AATT	8 cut(s)	126, 406, 457, 707, 936, 1296, 1350, 1354
TauI	GCSGC	1 cut(s)	1379
TfiI	GAWTC	8 cut(s)	75, 110, 322, 341, 905, 1132, 1370, 1571
TscAI	CASTG	2 cut(s)	1146, 1309
TseFI	GTSAC	1 cut(s)	1236
TseI	GCWGC	3 cut(s)	263, 662, 1379
Tsp45I	GTSAC	1 cut(s)	1236
TspDTI	ATGAA	4 cut(s)	6, 309, 359, 611
TspGWI	ACGGA	5 cut(s)	77, 317, 1200, 1486, 1503
TspRI	CASTG	2 cut(s)	1146, 1309
Vha464I	CTTAAG	1 cut(s)	602
VspI	ATTAAT	1 cut(s)	1353
XapI	RAATTY	3 cut(s)	126, 457, 707
XceI	RCATGY	1 cut(s)	924
XhoI	CTCGAG	1 cut(s)	332
Zsp2I	ATGCAT	1 cut(s)	1005

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.