Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0012094

Physical Location & Seq

Genomic Coordinates Forward (+)

12568603 .. 12572829

Gene Structure 4227 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g09810.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1770 bp

ATGCAATTTAAAGTAACTATATGGCGGTTACGGAAGCACTCGCGGTCGCGGGGAAGAGCAAAAGCCGAAGGGTTTAACGAGCGACTCATAAACCCGCAAATTGTAACAGAGCTTCCACTTCACCGCTGCCAATTCGCCGCCTGTCACCCGCCTCCAATCATGGAAGAAGGCACTTCATTTTTGTTCTCCGGCGTCAACTTCAACCGGAAAAAGTTTGCCACGGACTTCTCCAGATTCCAGAAGAACAAAGAGAGCGAGAAAGTGGTAGAAGATTCGAGCTTGTTCGAAATTCCAAAATCTGAGGCGGAGGTGGAGGCAGAGAAGGAGGAGCCAGTAGTGCCGGTTAAGAAGAGGAAGCGGAAGGGGCAGGTTTCCGAGGGTGTGGAGGGGATTAGTGTCTTTAAGACCTCAAAATCGGCGAAGGCAGCGAAAGCGGAGAAAGAGAAGCCTGGCAATGAACTAGCGGAGCAGAAGAAGAAGTTGAATCGGCAACTCGAGCGGGATTCGCTGACGCGAAAGAAGTATGACATTCATGTGTCCGGGAATAACATCCCTTCACCGCTTGATAATTTTGCAGACTTAACCTCAAGATATGGATGCGAGCCATATTTACTTCAGAATTTGGCAGAACTTGGATTTAAACAACCAACGCCAATCCAAAGGCAGGCTATTCCAGTCCTCTTATCTGGTCGAGAATGCTTTGCTTGTGCTCCGACTGGATCTGGAAAAACTCTTGCTTTTGTTTGTCCCATGCTAATGAAACTTAAGCATACATCTAAGGATGGCATCCGAGCTGTTATTCTTTGTCCAACACGTGAGTTAGCTGCTCAGACAACCAGAGAGTGCAAAAAGATGGCAAAAGGAAATAAATTCTACATCAAGTTAATGACCAAAGAGCTTGCAAGAAATGCCGATTTTTCAAAAATACGCTGTGATATACTCATTTCTACTCCACTTCGGTTACGGTTGGCTATCCAGAAAAAGAAGGTTGATTTAAGCAGGGTTGAGTATCTGGTATTGGATGAGTCTGATAAGCTTTTTGAGCTTGGCTTGTTAAAGCAGATTGATTCTGTGGTCAAAGCATGTTCAAACCCTTCAATTATTCGCTCCTTGTTTAGTGCTACTTTACCTGATTTCGTTGAGGAGCTTGCCCGCACTATAATGCATGATGCTGTTCGAGTTATCATTGGCAGGAAAAATACTGCTTCTGACACTATCAAGCAAAAATTGGTCTTTGCTGGAAGTGAAGAAGGGAAGCTATTAGCGCTTCGTCAATGTTTTAAGGAGAGTCTGAATCCACCAGTGCTGATATTTTTACAAAGCAAGGAGCGAGCTAAGGAACTCTACGGAGAACTGTTGTTTGAGAATGTAAGAGTTGGTGCCATACATTCTGATCTGTCACAAACACAGCGAGAAAATGCAGTAGATGACTTCAGAGCTGGAAAAACATGGGTTTTAATTGCCACTGATGTTATTGCTCGGGGTATGGATTTCAAAGGTGTCAACTGTGTGATTAATTATGATTTTCCAGATTCTGCCGCTGCCTACATTCACAGGATTGGTCGGTGTGGACGAGCAGGGAGGAGCGGAGAAGCTATTACTTTCTACACAGAGGTAGATATTCCATATCTACGGAATATAGCTAATGTGATGTCAACATCAGGTTGTGAAGTTCCGTCTTGGATCATGGCCTTGCGCAAACAGAAGTGGAAAAAACACCGACCCGGAAGAGAATCGATATCAACCCAACCGAAAGATGAGAAGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0010501 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031974 GO:0031981 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

590

Amino Acids

66.52

Weight (kDa)

9.53

Isoelectric Point (pI)

39.16

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012094)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09720 AT3G09720
fragaria_vesca	FvH4_6g15470
malus_domestica	MD04G1103000.v1.1 MD12G1123200.v1.1
prunus_persica	Prupe.6G240000_v2.0.a1 Prupe.6G240000_v2.0.a1
pyrus_communis	pycom04g09810 pycom12g12190
rosa_chinensis	RchiOBHm_Chr3g0468951
rosa_laevigata	RLG00000024397
rosa_multiflora	Rmu_co8069278.1_g000001 Rmu_co8291353.1_g000001
rosa_roxburghii	Rroxscaffold_6G00412430
rosa_rugosa	Rorug03G0097800
rosa_samantha	Rh3AG147400 Rh3BG170000 Rh3DG205000
rosa_wichuraiana	Rw3G013880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1697
Acc36I	ACCTGC	1 cut(s)	358
AccB1I	GGYRCC	1 cut(s)	1379
AccBSI	CCGCTC	2 cut(s)	499, 1587
AccII	CGCG	3 cut(s)	43, 49, 514
AclWI	GGATC	2 cut(s)	727, 1691
AcsI	RAATTY	3 cut(s)	288, 619, 869
AcuI	CTGAAG	2 cut(s)	599, 1417
AcvI	CACGTG	1 cut(s)	815
AcyI	GRCGYC	1 cut(s)	192
AfeI	AGCGCT	1 cut(s)	1266
AfiI	CCNNNNNNNGG	1 cut(s)	664
AflII	CTTAAG	1 cut(s)	764
AflIII	ACRYGT	1 cut(s)	812
AgsI	TTSAA	6 cut(s)	202, 484, 921, 1089, 1098, 1495
AjnI	CCWGG	1 cut(s)	448
Alw21I	GWGCWC	1 cut(s)	712
AlwI	GGATC	2 cut(s)	727, 1691
AlwNI	CAGNNNCTG	1 cut(s)	1535
Ama87I	CYCGRG	2 cut(s)	494, 1479
Aor51HI	AGCGCT	1 cut(s)	1266
AoxI	GGCC	1 cut(s)	1689
ApeKI	GCWGC	4 cut(s)	126, 425, 824, 1541
ApoI	RAATTY	3 cut(s)	288, 619, 869
AseI	ATTAAT	1 cut(s)	1515
AspLEI	GCGC	2 cut(s)	1267, 1698
AsuC2I	CCSGG	2 cut(s)	541, 1725
AsuHPI	GGTGA	3 cut(s)	113, 137, 549
AsuII	TTCGAA	1 cut(s)	285
AvaI	CYCGRG	2 cut(s)	494, 1479
BanI	GGYRCC	1 cut(s)	1379
BarI	GAAGNNNNNNTAC	2 cut(s)	96, 128
BbrPI	CACGTG	1 cut(s)	815
Bbv12I	GWGCWC	1 cut(s)	712
BbvI	GCAGC	4 cut(s)	113, 437, 811, 1528
BccI	CCATC	2 cut(s)	776, 847
BciT130I	CCWGG	1 cut(s)	450
BcnI	CCSGG	2 cut(s)	541, 1725
BfaI	CTAG	1 cut(s)	461
BfoI	RGCGCY	1 cut(s)	1268
BfrI	CTTAAG	1 cut(s)	764
BfuAI	ACCTGC	1 cut(s)	358
BisI	GCNGC	6 cut(s)	127, 138, 426, 825, 1539, 1542
BlsI	GCNGC	6 cut(s)	128, 139, 427, 826, 1540, 1543
Bme1390I	CCNGG	3 cut(s)	450, 541, 1725
BmeT110I	CYCGRG	2 cut(s)	494, 1479
BmiI	GGNNCC	2 cut(s)	330, 1381
BmrFI	CCNGG	3 cut(s)	450, 541, 1725
BmsI	GCATC	3 cut(s)	587, 795, 1159
BpmI	CTGGAG	1 cut(s)	214
Bpu10I	CCTNAGC	1 cut(s)	1335
Bpu14I	TTCGAA	1 cut(s)	285
BpuEI	CTTGAG	1 cut(s)	571
BpuMI	CCSGG	2 cut(s)	541, 1725
Bsa29I	ATCGAT	1 cut(s)	1736
BsaAI	YACGTR	1 cut(s)	815
BsaHI	GRCGYC	1 cut(s)	192
BsaJI	CCNNGG	2 cut(s)	219, 375
BsaWI	WCCGGW	1 cut(s)	204
Bsc4I	CCNNNNNNNGG	1 cut(s)	664
Bse118I	RCCGGY	1 cut(s)	340
Bse1I	ACTGG	4 cut(s)	332, 674, 721, 1301
Bse3DI	GCAATG	1 cut(s)	460
BseBI	CCWGG	1 cut(s)	450
BseCI	ATCGAT	1 cut(s)	1736
BseDI	CCNNGG	2 cut(s)	219, 375
BseGI	GGATG	5 cut(s)	549, 602, 786, 787, 1027
BseLI	CCNNNNNNNGG	1 cut(s)	664
BseMI	GCAATG	1 cut(s)	460
BseMII	CTCAG	2 cut(s)	291, 842
BseNI	ACTGG	4 cut(s)	332, 674, 721, 1301
BseRI	GAGGAG	3 cut(s)	341, 1157, 1597
BseXI	GCAGC	4 cut(s)	113, 437, 811, 1528
Bsh1236I	CGCG	3 cut(s)	43, 49, 514
Bsh1285I	CGRYCG	1 cut(s)	47
BshFI	GGCC	1 cut(s)	1691
BshNI	GGYRCC	1 cut(s)	1379
BshVI	ATCGAT	1 cut(s)	1736
BsiEI	CGRYCG	1 cut(s)	47
BsiHKAI	GWGCWC	1 cut(s)	712
BsiHKCI	CYCGRG	2 cut(s)	494, 1479
BsiSI	CCGG	5 cut(s)	189, 205, 341, 540, 1725
BslFI	GGGAC	1 cut(s)	732
BslI	CCNNNNNNNGG	1 cut(s)	664
BsmFI	GGGAC	1 cut(s)	732
BsmI	GAATGC	1 cut(s)	701
BsnI	GGCC	1 cut(s)	1691
BsoBI	CYCGRG	2 cut(s)	494, 1479
Bsp119I	TTCGAA	1 cut(s)	285
Bsp1286I	GDGCHC	1 cut(s)	712
Bsp143I	GATC	3 cut(s)	719, 1393, 1683
BspANI	GGCC	1 cut(s)	1691
BspCNI	CTCAG	2 cut(s)	292, 841
BspDI	ATCGAT	1 cut(s)	1736
BspFNI	CGCG	3 cut(s)	43, 49, 514
BspLI	GGNNCC	2 cut(s)	330, 1381
BspMI	ACCTGC	1 cut(s)	358
BspPI	GGATC	2 cut(s)	727, 1691
BspQI	GCTCTTC	1 cut(s)	49
BspT104I	TTCGAA	1 cut(s)	285
BspT107I	GGYRCC	1 cut(s)	1379
BspTI	CTTAAG	1 cut(s)	764
BsrBI	CCGCTC	2 cut(s)	499, 1587
BsrDI	GCAATG	1 cut(s)	460
BsrFI	RCCGGY	1 cut(s)	340
BsrI	ACTGG	4 cut(s)	332, 674, 721, 1301
BssAI	RCCGGY	1 cut(s)	340
BssECI	CCNNGG	2 cut(s)	219, 375
BssMI	GATC	3 cut(s)	719, 1393, 1683
BssNI	GRCGYC	1 cut(s)	192
Bst2UI	CCWGG	1 cut(s)	450
Bst4CI	ACNGT	3 cut(s)	966, 1356, 1508
Bst6I	CTCTTC	3 cut(s)	49, 344, 1723
BstACI	GRCGYC	1 cut(s)	192
BstAFI	CTTAAG	1 cut(s)	764
BstAPI	GCANNNNNTGC	1 cut(s)	908
BstBAI	YACGTR	1 cut(s)	815
BstBI	TTCGAA	1 cut(s)	285
BstC8I	GCNNGC	6 cut(s)	602, 666, 900, 1149, 1153, 1332
BstDEI	CTNAG	4 cut(s)	300, 777, 828, 1335
BstDSI	CCRYGG	1 cut(s)	219
BstF5I	GGATG	5 cut(s)	549, 602, 786, 787, 1027
BstFNI	CGCG	3 cut(s)	43, 49, 514
BstH2I	RGCGCY	1 cut(s)	1268
BstHHI	GCGC	2 cut(s)	1267, 1698
BstKTI	GATC	3 cut(s)	722, 1396, 1686
BstMBI	GATC	3 cut(s)	719, 1393, 1683
BstMCI	CGRYCG	1 cut(s)	47
BstMWI	GCNNNNNNNGC	9 cut(s)	337, 364, 425, 431, 496, 505, 908, 1042, 1264
BstNI	CCWGG	1 cut(s)	450
BstNSI	RCATGY	1 cut(s)	1086
BstSCI	CCNGG	3 cut(s)	448, 539, 1723
BstUI	CGCG	3 cut(s)	43, 49, 514
BstV1I	GCAGC	4 cut(s)	113, 437, 811, 1528
BstX2I	RGATCY	1 cut(s)	719
BstYI	RGATCY	1 cut(s)	719
Bsu15I	ATCGAT	1 cut(s)	1736
BsuRI	GGCC	1 cut(s)	1691
BsuTUI	ATCGAT	1 cut(s)	1736
BtgI	CCRYGG	1 cut(s)	219
BtsCI	GGATG	5 cut(s)	549, 602, 786, 787, 1027
BtsIMutI	CAGTG	2 cut(s)	1308, 1464
BveI	ACCTGC	1 cut(s)	358
Cac8I	GCNNGC	6 cut(s)	602, 666, 900, 1149, 1153, 1332
CaiI	CAGNNNCTG	1 cut(s)	1535
CfoI	GCGC	2 cut(s)	1267, 1698
Cfr10I	RCCGGY	1 cut(s)	340
ClaI	ATCGAT	1 cut(s)	1736
CseI	GACGC	2 cut(s)	181, 520
CviAII	CATG	7 cut(s)	160, 533, 751, 1083, 1166, 1449, 1687
DdeI	CTNAG	4 cut(s)	300, 777, 828, 1335
DpnI	GATC	3 cut(s)	721, 1395, 1685
DpnII	GATC	3 cut(s)	719, 1393, 1683
DraI	TTTAAA	2 cut(s)	10, 640
Eam1104I	CTCTTC	3 cut(s)	49, 344, 1723
EarI	CTCTTC	3 cut(s)	49, 344, 1723
EciI	GGCGGA	1 cut(s)	320
Eco32I	GATATC	1 cut(s)	1740
Eco47III	AGCGCT	1 cut(s)	1266
Eco57I	CTGAAG	2 cut(s)	599, 1417
Eco72I	CACGTG	1 cut(s)	815
Eco88I	CYCGRG	2 cut(s)	494, 1479
EcoRII	CCWGG	1 cut(s)	448
EcoRV	GATATC	1 cut(s)	1740
EcoT22I	ATGCAT	1 cut(s)	1167
FaeI	CATG	7 cut(s)	163, 536, 754, 1086, 1169, 1452, 1690
FaqI	GGGAC	1 cut(s)	732
FatI	CATG	7 cut(s)	159, 532, 750, 1082, 1165, 1448, 1686
FauI	CCCGC	5 cut(s)	42, 102, 156, 492, 1160
Fnu4HI	GCNGC	6 cut(s)	127, 138, 426, 825, 1539, 1542
FokI	GGATG	5 cut(s)	536, 609, 773, 794, 1034
Fsp4HI	GCNGC	6 cut(s)	127, 138, 426, 825, 1539, 1542
FspBI	CTAG	1 cut(s)	461
FspI	TGCGCA	1 cut(s)	1697
GlaI	GCGC	2 cut(s)	1266, 1697
GluI	GCNGC	6 cut(s)	127, 138, 426, 825, 1539, 1542
GsuI	CTGGAG	1 cut(s)	214
HaeII	RGCGCY	1 cut(s)	1268
HaeIII	GGCC	1 cut(s)	1691
HapII	CCGG	5 cut(s)	189, 205, 341, 540, 1725
HgaI	GACGC	2 cut(s)	181, 520
HhaI	GCGC	2 cut(s)	1267, 1698
Hin1I	GRCGYC	1 cut(s)	192
Hin1II	CATG	7 cut(s)	163, 536, 754, 1086, 1169, 1452, 1690
Hin6I	GCGC	2 cut(s)	1265, 1696
HinP1I	GCGC	2 cut(s)	1265, 1696
HincII	GTYRAC	3 cut(s)	196, 1504, 1656
HindII	GTYRAC	3 cut(s)	196, 1504, 1656
HindIII	AAGCTT	1 cut(s)	1034
HpaII	CCGG	5 cut(s)	189, 205, 341, 540, 1725
HphI	GGTGA	3 cut(s)	113, 137, 549
Hpy166II	GTNNAC	4 cut(s)	196, 1504, 1571, 1656
Hpy188III	TCNNGA	7 cut(s)	231, 238, 588, 692, 723, 976, 1529
Hpy8I	GTNNAC	4 cut(s)	196, 1504, 1571, 1656
HpyCH4III	ACNGT	3 cut(s)	966, 1356, 1508
HpyCH4IV	ACGT	1 cut(s)	814
HpyCH4V	TGCA	6 cut(s)	4, 575, 846, 902, 1165, 1421
HpyF10VI	GCNNNNNNNGC	9 cut(s)	337, 364, 425, 431, 496, 505, 908, 1042, 1264
HpyF3I	CTNAG	4 cut(s)	300, 777, 828, 1335
HpySE526I	ACGT	1 cut(s)	814
Hsp92I	GRCGYC	1 cut(s)	192
Hsp92II	CATG	7 cut(s)	163, 536, 754, 1086, 1169, 1452, 1690
HspAI	GCGC	2 cut(s)	1265, 1696
Kzo9I	GATC	3 cut(s)	719, 1393, 1683
LguI	GCTCTTC	1 cut(s)	49
LmnI	GCTCC	7 cut(s)	328, 466, 715, 1112, 1144, 1327, 1584
Lsp1109I	GCAGC	4 cut(s)	113, 437, 811, 1528
LweI	GCATC	3 cut(s)	587, 795, 1159
MaeI	CTAG	1 cut(s)	461
MaeII	ACGT	1 cut(s)	814
MaeIII	GTNAC	6 cut(s)	13, 27, 103, 143, 960, 1398
MalI	GATC	3 cut(s)	721, 1395, 1685
MbiI	CCGCTC	2 cut(s)	499, 1587
MboI	GATC	3 cut(s)	719, 1393, 1683
MboII	GAAGA	9 cut(s)	66, 176, 253, 281, 361, 484, 487, 1259, 1740
MflI	RGATCY	1 cut(s)	719
MhlI	GDGCHC	1 cut(s)	712
MlyI	GAGTC	3 cut(s)	78, 1034, 1297
MmeI	TCCRAC	2 cut(s)	737, 833
Mph1103I	ATGCAT	1 cut(s)	1167
MslI	CAYNNNNRTG	3 cut(s)	533, 755, 1160
MspA1I	CMGCKG	2 cut(s)	126, 1541
MspCI	CTTAAG	1 cut(s)	764
MspI	CCGG	5 cut(s)	189, 205, 341, 540, 1725
MspR9I	CCNGG	3 cut(s)	450, 541, 1725
Mva1269I	GAATGC	1 cut(s)	701
MvaI	CCWGG	1 cut(s)	450
MvnI	CGCG	3 cut(s)	43, 49, 514
MwoI	GCNNNNNNNGC	9 cut(s)	337, 364, 425, 431, 496, 505, 908, 1042, 1264
NciI	CCSGG	2 cut(s)	541, 1725
NdeII	GATC	3 cut(s)	719, 1393, 1683
NlaIII	CATG	7 cut(s)	163, 536, 754, 1086, 1169, 1452, 1690
NlaIV	GGNNCC	2 cut(s)	330, 1381
NmuCI	GTSAC	2 cut(s)	143, 1398
NsbI	TGCGCA	1 cut(s)	1697
NsiI	ATGCAT	1 cut(s)	1167
NspI	RCATGY	1 cut(s)	1086
NspV	TTCGAA	1 cut(s)	285
PaeR7I	CTCGAG	1 cut(s)	494
PciSI	GCTCTTC	1 cut(s)	49
PcsI	WCGNNNNNNNCGW	1 cut(s)	1570
PctI	GAATGC	1 cut(s)	701
PfeI	GAWTC	8 cut(s)	234, 272, 484, 503, 1067, 1294, 1532, 1733
PfoI	TCCNGGA	1 cut(s)	539
PkrI	GCNGC	6 cut(s)	128, 139, 427, 826, 1540, 1543
PleI	GAGTC	3 cut(s)	78, 1033, 1296
PmaCI	CACGTG	1 cut(s)	815
PmlI	CACGTG	1 cut(s)	815
PpsI	GAGTC	3 cut(s)	78, 1033, 1296
Ppu21I	YACGTR	1 cut(s)	815
PshBI	ATTAAT	1 cut(s)	1515
Psp6I	CCWGG	1 cut(s)	448
PspCI	CACGTG	1 cut(s)	815
PspGI	CCWGG	1 cut(s)	448
PspN4I	GGNNCC	2 cut(s)	330, 1381
PspXI	VCTCGAGB	1 cut(s)	494
PstNI	CAGNNNCTG	1 cut(s)	1535
PsuI	RGATCY	1 cut(s)	719
RseI	CAYNNNNRTG	3 cut(s)	533, 755, 1160
SapI	GCTCTTC	1 cut(s)	49
SatI	GCNGC	6 cut(s)	127, 138, 426, 825, 1539, 1542
Sau3AI	GATC	3 cut(s)	719, 1393, 1683
SchI	GAGTC	3 cut(s)	78, 1034, 1297
ScrFI	CCNGG	3 cut(s)	450, 541, 1725
SduI	GDGCHC	1 cut(s)	712
SfaNI	GCATC	3 cut(s)	587, 795, 1159
Sfr274I	CTCGAG	1 cut(s)	494
SfuI	TTCGAA	1 cut(s)	285
SlaI	CTCGAG	1 cut(s)	494
SmiMI	CAYNNNNRTG	3 cut(s)	533, 755, 1160
SmlI	CTYRAG	3 cut(s)	494, 586, 764
SmoI	CTYRAG	3 cut(s)	494, 586, 764
SspMI	CTAG	1 cut(s)	461
StyD4I	CCNGG	3 cut(s)	448, 539, 1723
TaaI	ACNGT	3 cut(s)	966, 1356, 1508
TaiI	ACGT	1 cut(s)	817
TaqI	TCGA	6 cut(s)	275, 285, 495, 691, 1177, 1736
TauI	GCSGC	2 cut(s)	140, 1541
TfiI	GAWTC	8 cut(s)	234, 272, 484, 503, 1067, 1294, 1532, 1733
TscAI	CASTG	2 cut(s)	1308, 1471
TseFI	GTSAC	2 cut(s)	143, 1398
TseI	GCWGC	4 cut(s)	126, 425, 824, 1541
Tsp45I	GTSAC	2 cut(s)	143, 1398
TspDTI	ATGAA	4 cut(s)	165, 471, 521, 773
TspGWI	ACGGA	5 cut(s)	46, 236, 1362, 1648, 1665
TspRI	CASTG	2 cut(s)	1308, 1471
Vha464I	CTTAAG	1 cut(s)	764
VspI	ATTAAT	1 cut(s)	1515
XapI	RAATTY	3 cut(s)	288, 619, 869
XceI	RCATGY	1 cut(s)	1086
XhoI	CTCGAG	1 cut(s)	494
XspI	CTAG	1 cut(s)	461
Zsp2I	ATGCAT	1 cut(s)	1167

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.