Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0012094

Physical Location & Seq

Genomic Coordinates Forward (+)

19752755 .. 19756930

Gene Structure 4176 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1123200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1620 bp

ATGGAAGAAGGCACTTCATTTTTGTTCGCCGGCGTCAACTTCAACCGGAAAAAGTTCTCCAGGGACTTCTCCAGATTCCAGAAGAAGAACATAGAGAGCGAGAAAGTGGTAGAAGATTCTAGCTTGTTCGGAATTACGGAATCTTCAACGGAGCAAGTGGCGGCGGAGGAGGAGGAGGTGCGAGCGGTGCCAGTTAAGAAGAGGAAGCGGAAGGGGGAGGTTTCTGAGGGTGTGGAGGGGATTAGTGTCTTTAAGACCTCAAAAATGGCGAAGGCAGCGAAAGCGGAGAAACAGAAGCCTGGAAATGAACTATCCGAGCATAAGAAGGAGTTAAATAGGCAACTTGAGCGGGATTCGCTGCTGCGAAAGAAGTATAACATTCATGTGTCCGGAAATAACATCCCTTCGCCACTTGATAGTTTTGCAGACTTAACCTCTAGATATGGGTGCGAGCCATATTTACTTCAGAATTTGGCGGAACTTGGATTTAAAGAACCAACGCCAATCCAAAGGCAGGCTATTCCAATCCTCTTATCTAGTCGGGAATGCTTTGCTTGTGCTCCGACTGGATCTGGAAAAACCCTTGCTTTTGTTTGTCCCATGCTAATGAAACTTAAGCATACATCTAAGGATGGCATCCGAGCTGTTATTCTGTGTCCTACACGAGAATTAGCTGCTCAGACAACCAGAGAGTGCAAAAAGATGGCAAAAGGAAATAAATTCTACATCAAATTAATGACTAAAGACCTGGCAAGAAATGCAGATTTTTCAAAAATACGCTGTGACATACTCATTTCTACTCCACTTCGGTTACGGTTGGCTATCCGGAAAAAGAAGGTTGATTTAAGCAGGGTTGAGTATCTGGTATTGGATGAGTCTGATAAGCTATTTGAGCTTGGCTTGTTAAAGCAGATTGACTCTGTGGTCAAAGCATGTTCAAACCCTTCAATTATTCGCTCCTTGTTTAGTGCTACTTTACCTGATTTCGTTGAGGAGCTTGCCCGCACTATAATGCATGATGCTGTTAGAGTTATCATTGGCAGGAAAAATACTGCTTCTGAGACTATCAAGCAAAAATTGGTCTTTGCTGGAAGTGAAGAGGGGAAGCTATTAGCACTTCGTCAAAGCTTTAAGGAGAGTCTGAATCCACCAGTGTTGATCTTTGTACAAAGCAAGGAGCGAGCTAAGGAACTCTACGGAGAACTGTTGTTTGAGAACATAAGAGTTGGTGCCATACATTCTGATATGTCCCAAGCACAGCGAGAAAATGCTGTAGATGACTTCAGAGCTGGGAAAACATGGGTTTTACTTGCCACTGATGTTATTGCTCGGGGTATGGATTTCAAAGGTGTCAACTGTGTAATTAATTATGATTTTCCAGATTCTGCAGCTGCGTACATTCACAGGATTGGTCGGTGCGGACGAGCAGGGAGGAGTGGAGAAGCTATTACTTTCTACACAGAGGAAGATATTCCATATCTTAAGAATATAGCTAATGTGATGTCAACATCAGGTTGTGAAGTTCCATCTTGGATCATGGCCTTGCGCAAACAGAAGTGGAAAAAGCACCGACCCCGAAGGGAATCGATATCAACACAACCGAAAGATGAGAAAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0010501 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031974 GO:0031981 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

540

Amino Acids

60.79

Weight (kDa)

9.41

Isoelectric Point (pI)

48.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	165 - 325	5.1e-09	Type III restriction enzyme, res subunit
DEAD	PF00270	167 - 332	6.1e-39	DEAD/DEAH box helicase
Helicase_C	PF00271	369 - 477	5.3e-32	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012094)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09720 AT3G09720
fragaria_vesca	FvH4_6g15470
malus_domestica	MD04G1103000.v1.1 MD12G1123200.v1.1
prunus_persica	Prupe.6G240000_v2.0.a1 Prupe.6G240000_v2.0.a1
pyrus_communis	pycom04g09810 pycom12g12190
rosa_chinensis	RchiOBHm_Chr3g0468951
rosa_laevigata	RLG00000024397
rosa_multiflora	Rmu_co8069278.1_g000001 Rmu_co8291353.1_g000001
rosa_roxburghii	Rroxscaffold_6G00412430
rosa_rugosa	Rorug03G0097800
rosa_samantha	Rh3AG147400 Rh3BG170000 Rh3DG205000
rosa_wichuraiana	Rw3G013880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	923
Acc16I	TGCGCA	1 cut(s)	1547
AccB1I	GGYRCC	2 cut(s)	187, 1229
AccBSI	CCGCTC	2 cut(s)	185, 349
AccIII	TCCGGA	2 cut(s)	389, 825
AciI	CCGC	9 cut(s)	161, 164, 185, 208, 284, 349, 476, 1003, 1419
AclWI	GGATC	2 cut(s)	577, 1541
AcsI	RAATTY	2 cut(s)	469, 719
AcuI	CTGAAG	2 cut(s)	449, 1267
AcyI	GRCGYC	1 cut(s)	33
AfaI	GTAC	2 cut(s)	1167, 1397
AfiI	CCNNNNNNNGG	1 cut(s)	514
AflII	CTTAAG	2 cut(s)	614, 1481
AgsI	TTSAA	6 cut(s)	43, 147, 771, 939, 948, 1345
AjnI	CCWGG	3 cut(s)	59, 298, 747
Alw21I	GWGCWC	1 cut(s)	562
Alw26I	GTCTC	1 cut(s)	1055
AlwI	GGATC	2 cut(s)	577, 1541
AlwNI	CAGNNNCTG	1 cut(s)	1385
Ama87I	CYCGRG	1 cut(s)	1329
Aor13HI	TCCGGA	2 cut(s)	389, 825
AoxI	GGCC	1 cut(s)	1539
ApeKI	GCWGC	6 cut(s)	275, 358, 361, 674, 1388, 1391
ApoI	RAATTY	2 cut(s)	469, 719
AseI	ATTAAT	2 cut(s)	734, 1365
Asp700I	GAANNNNTTC	2 cut(s)	53, 1470
AspLEI	GCGC	1 cut(s)	1548
AvaI	CYCGRG	1 cut(s)	1329
BanI	GGYRCC	2 cut(s)	187, 1229
BauI	CACGAG	1 cut(s)	663
Bbv12I	GWGCWC	1 cut(s)	562
BbvI	GCAGC	6 cut(s)	287, 345, 348, 661, 1378, 1400
BccI	CCATC	3 cut(s)	626, 697, 1534
BciT130I	CCWGG	3 cut(s)	61, 300, 749
BcoDI	GTCTC	1 cut(s)	1055
BfaI	CTAG	3 cut(s)	120, 438, 537
BfmI	CTRYAG	2 cut(s)	1272, 1386
BfrI	CTTAAG	2 cut(s)	614, 1481
BisI	GCNGC	7 cut(s)	162, 276, 359, 362, 675, 1389, 1392
BlsI	GCNGC	7 cut(s)	163, 277, 360, 363, 676, 1390, 1393
Bme1390I	CCNGG	3 cut(s)	61, 300, 749
BmeT110I	CYCGRG	1 cut(s)	1329
BmiI	GGNNCC	2 cut(s)	189, 1231
BmrFI	CCNGG	3 cut(s)	61, 300, 749
BmsI	GCATC	2 cut(s)	645, 1009
BpmI	CTGGAG	2 cut(s)	43, 55
Bpu10I	CCTNAGC	1 cut(s)	1185
BpuEI	CTTGAG	1 cut(s)	365
Bsa29I	ATCGAT	1 cut(s)	1586
BsaHI	GRCGYC	1 cut(s)	33
BsaJI	CCNNGG	1 cut(s)	60
BsaWI	WCCGGW	3 cut(s)	45, 389, 825
Bsc4I	CCNNNNNNNGG	1 cut(s)	514
Bse118I	RCCGGY	1 cut(s)	29
Bse1I	ACTGG	3 cut(s)	191, 571, 1151
BseAI	TCCGGA	2 cut(s)	389, 825
BseBI	CCWGG	3 cut(s)	61, 300, 749
BseCI	ATCGAT	1 cut(s)	1586
BseDI	CCNNGG	1 cut(s)	60
BseGI	GGATG	4 cut(s)	399, 636, 637, 877
BseLI	CCNNNNNNNGG	1 cut(s)	514
BseMII	CTCAG	3 cut(s)	216, 692, 1050
BseNI	ACTGG	3 cut(s)	191, 571, 1151
BseRI	GAGGAG	5 cut(s)	182, 185, 188, 1007, 1447
BseXI	GCAGC	6 cut(s)	287, 345, 348, 661, 1378, 1400
BseYI	CCCAGC	1 cut(s)	1289
BshFI	GGCC	1 cut(s)	1541
BshNI	GGYRCC	2 cut(s)	187, 1229
BshVI	ATCGAT	1 cut(s)	1586
BsiHKAI	GWGCWC	1 cut(s)	562
BsiHKCI	CYCGRG	1 cut(s)	1329
BsiSI	CCGG	4 cut(s)	30, 46, 390, 826
BslFI	GGGAC	3 cut(s)	77, 582, 1234
BslI	CCNNNNNNNGG	1 cut(s)	514
BsmAI	GTCTC	1 cut(s)	1055
BsmFI	GGGAC	3 cut(s)	77, 582, 1234
BsmI	GAATGC	1 cut(s)	551
BsnI	GGCC	1 cut(s)	1541
BsoBI	CYCGRG	1 cut(s)	1329
Bsp1286I	GDGCHC	1 cut(s)	562
Bsp13I	TCCGGA	2 cut(s)	389, 825
Bsp1407I	TGTACA	1 cut(s)	1165
Bsp143I	GATC	3 cut(s)	569, 1158, 1533
BspACI	CCGC	9 cut(s)	161, 164, 185, 208, 284, 349, 476, 1003, 1419
BspANI	GGCC	1 cut(s)	1541
BspCNI	CTCAG	3 cut(s)	217, 691, 1051
BspDI	ATCGAT	1 cut(s)	1586
BspEI	TCCGGA	2 cut(s)	389, 825
BspLI	GGNNCC	2 cut(s)	189, 1231
BspMAI	CTGCAG	1 cut(s)	1390
BspPI	GGATC	2 cut(s)	577, 1541
BspT107I	GGYRCC	2 cut(s)	187, 1229
BspTI	CTTAAG	2 cut(s)	614, 1481
BsrBI	CCGCTC	2 cut(s)	185, 349
BsrFI	RCCGGY	1 cut(s)	29
BsrGI	TGTACA	1 cut(s)	1165
BsrI	ACTGG	3 cut(s)	191, 571, 1151
BssAI	RCCGGY	1 cut(s)	29
BssECI	CCNNGG	1 cut(s)	60
BssMI	GATC	3 cut(s)	569, 1158, 1533
BssNI	GRCGYC	1 cut(s)	33
BssSI	CACGAG	1 cut(s)	663
Bst2BI	CACGAG	1 cut(s)	663
Bst2UI	CCWGG	3 cut(s)	61, 300, 749
Bst4CI	ACNGT	3 cut(s)	816, 1206, 1358
Bst6I	CTCTTC	2 cut(s)	194, 1092
BstACI	GRCGYC	1 cut(s)	33
BstAFI	CTTAAG	2 cut(s)	614, 1481
BstAPI	GCANNNNNTGC	1 cut(s)	758
BstAUI	TGTACA	1 cut(s)	1165
BstC8I	GCNNGC	7 cut(s)	31, 183, 452, 516, 999, 1003, 1182
BstDEI	CTNAG	5 cut(s)	225, 627, 678, 1059, 1185
BstF5I	GGATG	4 cut(s)	399, 636, 637, 877
BstHHI	GCGC	1 cut(s)	1548
BstKTI	GATC	3 cut(s)	572, 1161, 1536
BstMAI	GTCTC	1 cut(s)	1055
BstMBI	GATC	3 cut(s)	569, 1158, 1533
BstMWI	GCNNNNNNNGC	7 cut(s)	187, 275, 281, 346, 355, 758, 892
BstNI	CCWGG	3 cut(s)	61, 300, 749
BstNSI	RCATGY	1 cut(s)	936
BstSCI	CCNGG	3 cut(s)	59, 298, 747
BstSFI	CTRYAG	2 cut(s)	1272, 1386
BstV1I	GCAGC	6 cut(s)	287, 345, 348, 661, 1378, 1400
BstX2I	RGATCY	1 cut(s)	569
BstYI	RGATCY	1 cut(s)	569
Bsu15I	ATCGAT	1 cut(s)	1586
BsuRI	GGCC	1 cut(s)	1541
BsuTUI	ATCGAT	1 cut(s)	1586
BtsCI	GGATG	4 cut(s)	399, 636, 637, 877
BtsIMutI	CAGTG	2 cut(s)	1158, 1314
Cac8I	GCNNGC	7 cut(s)	31, 183, 452, 516, 999, 1003, 1182
CaiI	CAGNNNCTG	1 cut(s)	1385
CfoI	GCGC	1 cut(s)	1548
Cfr10I	RCCGGY	1 cut(s)	29
ClaI	ATCGAT	1 cut(s)	1586
CseI	GACGC	1 cut(s)	22
Csp6I	GTAC	2 cut(s)	1166, 1396
CspCI	CAANNNNNGTGG	2 cut(s)	1137, 1172
CviAII	CATG	6 cut(s)	383, 601, 933, 1016, 1299, 1537
CviQI	GTAC	2 cut(s)	1166, 1396
DdeI	CTNAG	5 cut(s)	225, 627, 678, 1059, 1185
DpnI	GATC	3 cut(s)	571, 1160, 1535
DpnII	GATC	3 cut(s)	569, 1158, 1533
DraI	TTTAAA	1 cut(s)	490
DrdI	GACNNNNNNGTC	1 cut(s)	923
DseDI	GACNNNNNNGTC	1 cut(s)	923
Eam1104I	CTCTTC	2 cut(s)	194, 1092
EarI	CTCTTC	2 cut(s)	194, 1092
EciI	GGCGGA	2 cut(s)	179, 491
Eco32I	GATATC	1 cut(s)	1590
Eco57I	CTGAAG	2 cut(s)	449, 1267
Eco88I	CYCGRG	1 cut(s)	1329
EcoRII	CCWGG	3 cut(s)	59, 298, 747
EcoRV	GATATC	1 cut(s)	1590
EcoT22I	ATGCAT	1 cut(s)	1017
FaeI	CATG	6 cut(s)	386, 604, 936, 1019, 1302, 1540
FaqI	GGGAC	3 cut(s)	77, 582, 1234
FatI	CATG	6 cut(s)	382, 600, 932, 1015, 1298, 1536
FauI	CCCGC	2 cut(s)	342, 1010
Fnu4HI	GCNGC	7 cut(s)	162, 276, 359, 362, 675, 1389, 1392
FokI	GGATG	4 cut(s)	386, 623, 644, 884
Fsp4HI	GCNGC	7 cut(s)	162, 276, 359, 362, 675, 1389, 1392
FspBI	CTAG	3 cut(s)	120, 438, 537
FspI	TGCGCA	1 cut(s)	1547
GlaI	GCGC	1 cut(s)	1547
GluI	GCNGC	7 cut(s)	162, 276, 359, 362, 675, 1389, 1392
GsaI	CCCAGC	1 cut(s)	1293
GsuI	CTGGAG	2 cut(s)	43, 55
HaeIII	GGCC	1 cut(s)	1541
HapII	CCGG	4 cut(s)	30, 46, 390, 826
HgaI	GACGC	1 cut(s)	22
HhaI	GCGC	1 cut(s)	1548
Hin1I	GRCGYC	1 cut(s)	33
Hin1II	CATG	6 cut(s)	386, 604, 936, 1019, 1302, 1540
Hin6I	GCGC	1 cut(s)	1546
HinP1I	GCGC	1 cut(s)	1546
HincII	GTYRAC	3 cut(s)	37, 1354, 1506
HindII	GTYRAC	3 cut(s)	37, 1354, 1506
HindIII	AAGCTT	1 cut(s)	1126
HpaII	CCGG	4 cut(s)	30, 46, 390, 826
Hpy166II	GTNNAC	3 cut(s)	37, 1354, 1506
Hpy188III	TCNNGA	8 cut(s)	72, 79, 390, 438, 542, 573, 826, 1379
Hpy8I	GTNNAC	3 cut(s)	37, 1354, 1506
HpyAV	CCTTC	7 cut(s)	205, 265, 319, 414, 829, 954, 1572
HpyCH4III	ACNGT	3 cut(s)	816, 1206, 1358
HpyCH4V	TGCA	5 cut(s)	425, 696, 761, 1015, 1388
HpyF10VI	GCNNNNNNNGC	7 cut(s)	187, 275, 281, 346, 355, 758, 892
HpyF3I	CTNAG	5 cut(s)	225, 627, 678, 1059, 1185
Hsp92I	GRCGYC	1 cut(s)	33
Hsp92II	CATG	6 cut(s)	386, 604, 936, 1019, 1302, 1540
HspAI	GCGC	1 cut(s)	1546
Kpn2I	TCCGGA	2 cut(s)	389, 825
KroI	GCCGGC	1 cut(s)	29
KroNI	GCCGGC	1 cut(s)	31
Kzo9I	GATC	3 cut(s)	569, 1158, 1533
LmnI	GCTCC	5 cut(s)	151, 565, 962, 994, 1177
Lsp1109I	GCAGC	6 cut(s)	287, 345, 348, 661, 1378, 1400
LweI	GCATC	2 cut(s)	645, 1009
MaeI	CTAG	3 cut(s)	120, 438, 537
MaeIII	GTNAC	2 cut(s)	782, 810
MalI	GATC	3 cut(s)	571, 1160, 1535
MbiI	CCGCTC	2 cut(s)	185, 349
MboI	GATC	3 cut(s)	569, 1158, 1533
MboII	GAAGA	8 cut(s)	17, 94, 97, 125, 135, 211, 1109, 1478
MflI	RGATCY	1 cut(s)	569
MhlI	GDGCHC	1 cut(s)	562
MluCI	AATT	9 cut(s)	132, 469, 668, 719, 731, 948, 1076, 1362, 1366
MlyI	GAGTC	3 cut(s)	884, 911, 1147
MmeI	TCCRAC	1 cut(s)	587
Mph1103I	ATGCAT	1 cut(s)	1017
MreI	CGCCGGCG	1 cut(s)	29
MroI	TCCGGA	2 cut(s)	389, 825
MroNI	GCCGGC	1 cut(s)	29
MroXI	GAANNNNTTC	2 cut(s)	53, 1470
MslI	CAYNNNNRTG	3 cut(s)	383, 605, 1010
MspA1I	CMGCKG	1 cut(s)	1391
MspCI	CTTAAG	2 cut(s)	614, 1481
MspI	CCGG	4 cut(s)	30, 46, 390, 826
MspR9I	CCNGG	3 cut(s)	61, 300, 749
Mva1269I	GAATGC	1 cut(s)	551
MvaI	CCWGG	3 cut(s)	61, 300, 749
MwoI	GCNNNNNNNGC	7 cut(s)	187, 275, 281, 346, 355, 758, 892
NaeI	GCCGGC	1 cut(s)	31
NdeII	GATC	3 cut(s)	569, 1158, 1533
NgoMIV	GCCGGC	1 cut(s)	29
NlaIII	CATG	6 cut(s)	386, 604, 936, 1019, 1302, 1540
NlaIV	GGNNCC	2 cut(s)	189, 1231
NmuCI	GTSAC	1 cut(s)	782
NsbI	TGCGCA	1 cut(s)	1547
NsiI	ATGCAT	1 cut(s)	1017
NspI	RCATGY	1 cut(s)	936
PcsI	WCGNNNNNNNCGW	1 cut(s)	1420
PctI	GAATGC	1 cut(s)	551
PdiI	GCCGGC	1 cut(s)	31
PdmI	GAANNNNTTC	2 cut(s)	53, 1470
PfeI	GAWTC	7 cut(s)	75, 116, 140, 353, 1144, 1382, 1583
PkrI	GCNGC	7 cut(s)	163, 277, 360, 363, 676, 1390, 1393
PleI	GAGTC	3 cut(s)	883, 911, 1146
PpsI	GAGTC	3 cut(s)	883, 911, 1146
PshBI	ATTAAT	2 cut(s)	734, 1365
Psp6I	CCWGG	3 cut(s)	59, 298, 747
PspFI	CCCAGC	1 cut(s)	1289
PspGI	CCWGG	3 cut(s)	59, 298, 747
PspN4I	GGNNCC	2 cut(s)	189, 1231
PstI	CTGCAG	1 cut(s)	1390
PstNI	CAGNNNCTG	1 cut(s)	1385
PsuI	RGATCY	1 cut(s)	569
PvuII	CAGCTG	1 cut(s)	1391
RsaI	GTAC	2 cut(s)	1167, 1397
RsaNI	GTAC	2 cut(s)	1166, 1396
RseI	CAYNNNNRTG	3 cut(s)	383, 605, 1010
SatI	GCNGC	7 cut(s)	162, 276, 359, 362, 675, 1389, 1392
Sau3AI	GATC	3 cut(s)	569, 1158, 1533
SchI	GAGTC	3 cut(s)	884, 911, 1147
ScrFI	CCNGG	3 cut(s)	61, 300, 749
SduI	GDGCHC	1 cut(s)	562
SfaNI	GCATC	2 cut(s)	645, 1009
SfcI	CTRYAG	2 cut(s)	1272, 1386
SgrAI	CRCCGGYG	1 cut(s)	29
SmiMI	CAYNNNNRTG	3 cut(s)	383, 605, 1010
SmlI	CTYRAG	3 cut(s)	344, 614, 1481
SmoI	CTYRAG	3 cut(s)	344, 614, 1481
Sse9I	AATT	9 cut(s)	132, 469, 668, 719, 731, 948, 1076, 1362, 1366
SsiI	CCGC	9 cut(s)	161, 164, 185, 208, 284, 349, 476, 1003, 1419
SspMI	CTAG	3 cut(s)	120, 438, 537
StyD4I	CCNGG	3 cut(s)	59, 298, 747
TaaI	ACNGT	3 cut(s)	816, 1206, 1358
TaqI	TCGA	1 cut(s)	1586
TasI	AATT	9 cut(s)	132, 469, 668, 719, 731, 948, 1076, 1362, 1366
TatI	WGTACW	1 cut(s)	1165
TauI	GCSGC	1 cut(s)	164
TfiI	GAWTC	7 cut(s)	75, 116, 140, 353, 1144, 1382, 1583
TscAI	CASTG	2 cut(s)	1158, 1321
TseFI	GTSAC	1 cut(s)	782
TseI	GCWGC	6 cut(s)	275, 358, 361, 674, 1388, 1391
Tsp45I	GTSAC	1 cut(s)	782
TspDTI	ATGAA	4 cut(s)	6, 321, 371, 623
TspGWI	ACGGA	3 cut(s)	152, 164, 1212
TspRI	CASTG	2 cut(s)	1158, 1321
Vha464I	CTTAAG	2 cut(s)	614, 1481
VspI	ATTAAT	2 cut(s)	734, 1365
XapI	RAATTY	2 cut(s)	469, 719
XbaI	TCTAGA	1 cut(s)	437
XceI	RCATGY	1 cut(s)	936
XmnI	GAANNNNTTC	2 cut(s)	53, 1470
XspI	CTAG	3 cut(s)	120, 438, 537
Zsp2I	ATGCAT	1 cut(s)	1017

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.