Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0009486

Physical Location & Seq

Genomic Coordinates Forward (+)

20571164 .. 20577710

Gene Structure 6547 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1119500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3108 bp

ATGGCAATCACCCCTGATCAGACTAGGAAAGTTGGGATGGGGCTGTCACCATCCCCATCTCCTTTTCTTACTCCTCGTCCCGAAAGGCGGCGCAAAACGCTTGAGTACAATCCAAATCGTCAAGACAAGGATAAAGAGGTTAATGTGCAAGTTCTTCTCAGATGCAGGCCTTTAAGTGATGAAGAGCAAAAGTTGAATATCCAGAAGGTGGTATCCTGTAATGAACAGAAAAGAGAGGTCACTGTTATGCAAAGTCTAAATAACAAGCAAGTAGATAAACTGTTCACTTTTGACAGGGTTTTTGGGCCAAAAGCACAGCAAAAATCAATATATGAGCAAGCCATTTCCCCGATAGTTAATGAAGCTCTCGAGGGATTCAACTGCACTGTTTTCGCTTATGGGCAGACGGGAACTGGTAAAACTTACACAATGGAGGGCGGGATGAGAAATAAGAGTGGCAATTTGCCTACTGAAGCTGGTGTTATTCCACGGGCTGTTCGTCAAATTTTTGATACACTTGAAGCACAAGATGCGGACTATAGTGTGAAAGTGACGTTCTTGGAAATATATAACGAAGAAATAACTGATTTACTAGCGCCTGATGAGAGTCCAAGAACTGCAGACGACAGGCAAAAGAAATCTATTTCATTGATGGAGGATGGAAAGGGTTGTGTGATAGTAAGAGGCCTTGAAGAAGAATATGTGTACAGTGTAAATGAAATTTATACTCTCTTGGAACGAGGGTCTGCCAAGAGGCGTACAGCAGACACTCTGTTGAACAAGCGCAGCAGTCGCTCTCATTCTGTCTTTTCCATTACTGTCCTCATAAAAGAAGCAACAGTTGGTGATGAGGAGCTAATTAAATGTGGCAAAATTAATCTTGTGGATCTGGCAGGATCGGAAAACATATCTCGTTCAGGTGCACGAGAGGGACGTGCAAGAGAAGCAGGGGAAATAAATAAGAGCTTGCTCACGCTGGGCCGTGTCATAAATGCACTAGTGGAACATTCCACTCATATACCTTACAGGGACAGTAAGCTTACAAGACTTTTAAGAGATTCTTTGGGAGGAAAAACAAAAACCTGCATCATTGCCACAATTTCTCCAACTGCTTCATGCATGGATGAAACTCTAAGCACACTGGATTATGCCTATCGTGCCAAGAACATTAAAAACAAACCCGAGGCAAACCAGAAAATGTCAAAACCTGTGCTGCTCAAGGAATTGTATTCAGAGATTGAGAGAATGAAAGAAGATATTCGAGCGGCACGGGAAAAGAACGGTGTTTATATTCCGCATGAAAGATTTGTCCAGGATGAAGCTGAAAAAAAGGCTAGGATTGAGAGAATAGAGCAATTGGAGAATGACCTCAACCTCACAGAAAAGCAAGCGGAAAGTTTTCGTGATCTGTATATCAGCGAACAAGAACAGAAGCTGGATTTACAAAGTGAGCTCAAGGACTGCAAGATAAATCTGGATAACTGTAACAAGGCTCTGCTTGAACTTCAAGAGGATTACCAAATAGCCACCTCAACCTTGAAAGAGAAGGAGCATATCATCTCCAAAATGCTATTCTCAGAAAATCTTTTAATTGGACGGGCAAAGGAGTTGCGCAGTGATTTGAAGAACGCATCAGAGGAAATGAATTCCCTCTATGAAAAATTAGATCAGAAAGACAAGATGGAAGAAGAAAATCAGAGCTTGGTTTTGACATTTGGTTCTCAACTTGATCGGAGTTTGAAAGACCTACATAAGATCATCCTGGGGTCTGTTTCTCAACAGCAGTATCAACTAAGATGCATGGAGGAACATGTTCAGTCATACCTTGCGAGTAAATGTGATGCAGCACAGGTACTGGAATCCAGGATTAAGAAGATAACAAAGACATATAGTTCTGGAGTAACAACCTTGAAGGAGCTGACCAACATGCTGCAAAAGAAAGCATCATCTGACCTGGACCAAATAAATGCTAAGATGTTATCACATACAGAGGCTGTTGAGAAGTTTCTTGAAACTGCGGTTAAGGAAGCCAACGAGGTCGTCCAAGATATTCAGCACTCTCTTGATGAGCAGAAACAGCTATTGGCTTTCTCCACTCAACAACAAGAGGAGGGATTGCAAAGAAGCTTGCTGTCAGCACAAGTAATTTCGAAGGCCACTGGTAACTTTTTTGATGACCTCCATGACCGCGCTTGTCAAGTTATGAGAAATCTTGAAGAAACCCAAATTCAACGAGTCAACCAATTGGTGAATTTTGAGAAGATGTTTAAGGAAGAAGCTTGTAGAGAGGAAAAACATGCTATGGAAAAAATTGCAGTAATTATTGGAACATTGACATCTAAGAAAGCCTTTATGGTATCGGAGGCATCAACCAACATCCAGGAAAAGAGTAAAGAAGACAACACCAGGCTGCAAAAGGCAGTCTTTAACATGCAACAAGTGGCATCGGATGCTAGGACGGAATTGAGCGAATATTTCGGAAAGGTGGAATGTGATTTTATGAAAGACACATTCTCAGCAGTTGAATCTCAGGCAATCATGGAAAACTGCCTCCAAGAGTGCTCAGAGAGAGTGGGTTACTCTGGGAAACAGTGGGAAAATGCCCAGTCAGCCATAAATACTCTTAATCAGAACAGTGCTGCAAATATCGAATCTACTGTGAAGGAAAATATCTCTGCGAACCATTCTGCACATGAAAAGTTTGTTTCTGCATCTTCCCGTGTGGATTCAGATTTCAGTGCTTTAGCCTCCGACACATTGTCATGCGTTAATGATTCGCTAATGCTGGACTATGTAAAGACAAAGGAAATAGACTCCATGACGGCTGTATGCGTTGATCAGCTTAAATCAGTACAAGAAAAGCATGGTGATGGCGTATCAACTATACGAAATCAAGCGGAAAAGTGCTTTGTAGAAGATTATTTGGTGGATAAGCATGCTGGTGCGACCAAGAAACAAGTCATTGCTGTCCCCAGCTTGGAATCTATCGAGGAGATGAGAAGCTCCGTCGAAGTCAAAGAAGACGGTTCATCTAATAACAAATTAAAACGGAGCCAAATGGAAGGCAAGATCCCAGGTCCTAGAACTCCTTTCGCGGATGTCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0008574 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1036

Amino Acids

116.84

Weight (kDa)

5.6

Isoelectric Point (pI)

48.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	44 - 198	7.6e-28	Microtubule binding
Kinesin	PF00225	54 - 390	1.4e-117	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009486)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37420 AT2G37420
fragaria_vesca	FvH4_6g14420
malus_domestica	MD04G1119500.v1.1 MD12G1136800.v1.1
prunus_persica	Prupe.6G249200_v2.0.a1
pyrus_communis	pycom04g10890 pycom04g10900 pycom12g13130
rosa_chinensis	RchiOBHm_Chr3g0467551
rosa_laevigata	RLG00000024492
rosa_multiflora	Rmu_sc0000286.1_g000001 Rmu_sc0027582.1_g000001
rosa_roxburghii	Rroxscaffold_6G00413600
rosa_rugosa	Rorug03G0087900 Rorug03G0088000 Rorug03G0088100
rosa_samantha	Rh3AG135900 Rh3BG157700 Rh3CG157600 Rh3DG158500
rosa_wichuraiana	Rw3G012910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1613
Acc36I	ACCTGC	1 cut(s)	1091
AccB7I	CCANNNNNTGG	1 cut(s)	208
AccBSI	CCGCTC	1 cut(s)	1265
AccII	CGCG	2 cut(s)	2190, 3095
AclWI	GGATC	3 cut(s)	894, 904, 3064
AcsI	RAATTY	5 cut(s)	504, 720, 1645, 2226, 2251
AcuI	CTGAAG	1 cut(s)	492
AfaI	GTAC	5 cut(s)	107, 707, 760, 1854, 2853
AfiI	CCNNNNNNNGG	3 cut(s)	87, 208, 2977
AflIII	ACRYGT	1 cut(s)	1810
AhdI	GACNNNNNGTC	1 cut(s)	2758
AhlI	ACTAGT	1 cut(s)	997
AjiI	CACGTC	1 cut(s)	935
AjnI	CCWGG	7 cut(s)	1311, 1761, 1862, 1953, 2379, 2405, 3073
AjuI	GAANNNNNNNTTGG	6 cut(s)	825, 857, 1556, 1588, 2066, 2098
Alw21I	GWGCWC	3 cut(s)	925, 1455, 2564
Alw44I	GTGCAC	1 cut(s)	921
AlwI	GGATC	3 cut(s)	894, 904, 3064
AlwNI	CAGNNNCTG	3 cut(s)	1435, 1855, 1994
Ama87I	CYCGRG	2 cut(s)	368, 1181
AoxI	GGCC	5 cut(s)	167, 305, 685, 979, 2154
ApaLI	GTGCAC	1 cut(s)	921
ApeKI	GCWGC	6 cut(s)	786, 1213, 1844, 1930, 2410, 2639
ApoI	RAATTY	5 cut(s)	504, 720, 1645, 2226, 2251
ArsI	GACNNNNNNTTYG	2 cut(s)	284, 316
AseI	ATTAAT	1 cut(s)	876
Asp700I	GAANNNNTTC	3 cut(s)	1257, 1398, 1812
AspLEI	GCGC	5 cut(s)	93, 598, 786, 1614, 2192
AspS9I	GGNCC	4 cut(s)	305, 979, 1957, 3077
AsuHPI	GGTGA	4 cut(s)	39, 857, 2260, 2879
AsuII	TTCGAA	1 cut(s)	2150
AvaI	CYCGRG	2 cut(s)	368, 1181
AvaII	GGWCC	2 cut(s)	1957, 3077
BaeGI	GKGCMC	1 cut(s)	925
BaeI	ACNNNNGTAYC	2 cut(s)	1769, 1802
BanII	GRGCYC	1 cut(s)	1455
BauI	CACGAG	1 cut(s)	924
BbsI	GAAGAC	2 cut(s)	2403, 3027
Bbv12I	GWGCWC	3 cut(s)	925, 1455, 2564
BbvI	GCAGC	6 cut(s)	798, 1200, 1856, 1917, 2397, 2626
BccI	CCATC	7 cut(s)	31, 58, 64, 646, 653, 1675, 2864
BceAI	ACGGC	2 cut(s)	966, 2838
BcgI	CGANNNNNNTGC	2 cut(s)	373, 407
BciT130I	CCWGG	7 cut(s)	1313, 1763, 1864, 1955, 2381, 2407, 3075
BciVI	GTATCC	1 cut(s)	223
BclI	TGATCA	2 cut(s)	16, 2836
BcuI	ACTAGT	1 cut(s)	997
BfaI	CTAG	6 cut(s)	24, 593, 998, 1335, 2454, 3081
BfmI	CTRYAG	2 cut(s)	538, 618
BfoI	RGCGCY	1 cut(s)	599
BfuAI	ACCTGC	1 cut(s)	1091
BfuI	GTATCC	1 cut(s)	223
BisI	GCNGC	8 cut(s)	89, 787, 1214, 1266, 1845, 1931, 2411, 2640
BlsI	GCNGC	8 cut(s)	90, 788, 1215, 1267, 1846, 1932, 2412, 2641
Bme1390I	CCNGG	7 cut(s)	1313, 1763, 1864, 1955, 2381, 2407, 3075
Bme18I	GGWCC	2 cut(s)	1957, 3077
BmeRI	GACNNNNNGTC	1 cut(s)	2758
BmeT110I	CYCGRG	2 cut(s)	368, 1181
BmgBI	CACGTC	1 cut(s)	935
BmgT120I	GGNCC	4 cut(s)	305, 979, 1957, 3077
BmiI	GGNNCC	1 cut(s)	3053
BmrFI	CCNGG	7 cut(s)	1313, 1763, 1864, 1955, 2381, 2407, 3075
BmrI	ACTGGG	1 cut(s)	2599
BmuI	ACTGGG	1 cut(s)	2599
BpiI	GAAGAC	2 cut(s)	2403, 3027
BpmI	CTGGAG	1 cut(s)	1917
Bpu14I	TTCGAA	1 cut(s)	2150
BpuEI	CTTGAG	3 cut(s)	122, 1202, 1439
BsaJI	CCNNGG	4 cut(s)	488, 1182, 1762, 3073
BsaXI	ACNNNNNCTCC	2 cut(s)	1087, 1117
Bsc4I	CCNNNNNNNGG	3 cut(s)	87, 208, 2977
Bse1I	ACTGG	5 cut(s)	418, 1146, 1860, 2164, 2605
Bse3DI	GCAATG	2 cut(s)	1089, 2961
BseBI	CCWGG	7 cut(s)	1313, 1763, 1864, 1955, 2381, 2407, 3075
BseDI	CCNNGG	4 cut(s)	488, 1182, 1762, 3073
BseLI	CCNNNNNNNGG	3 cut(s)	87, 208, 2977
BseMI	GCAATG	2 cut(s)	1089, 2961
BseMII	CTCAG	5 cut(s)	172, 1590, 2529, 2543, 2577
BseNI	ACTGG	5 cut(s)	418, 1146, 1860, 2164, 2605
BseRI	GAGGAG	4 cut(s)	63, 866, 2123, 3005
BseSI	GKGCMC	1 cut(s)	925
BseXI	GCAGC	6 cut(s)	798, 1200, 1856, 1917, 2397, 2626
BseYI	CCCAGC	2 cut(s)	976, 2972
BsgI	GTGCAG	2 cut(s)	367, 2673
Bsh1236I	CGCG	2 cut(s)	2190, 3095
BshFI	GGCC	5 cut(s)	169, 307, 687, 981, 2156
BsiHKAI	GWGCWC	3 cut(s)	925, 1455, 2564
BsiHKCI	CYCGRG	2 cut(s)	368, 1181
BslFI	GGGAC	4 cut(s)	63, 945, 1043, 2954
BslI	CCNNNNNNNGG	3 cut(s)	87, 208, 2977
BsmFI	GGGAC	4 cut(s)	63, 945, 1043, 2954
BsnI	GGCC	5 cut(s)	169, 307, 687, 981, 2156
BsoBI	CYCGRG	2 cut(s)	368, 1181
Bsp119I	TTCGAA	1 cut(s)	2150
Bsp1286I	GDGCHC	3 cut(s)	925, 1455, 2564
Bsp1407I	TGTACA	1 cut(s)	705
Bsp143I	GATC	9 cut(s)	16, 886, 896, 1405, 1666, 1729, 1755, 2836, 3069
BspANI	GGCC	5 cut(s)	169, 307, 687, 981, 2156
BspCNI	CTCAG	5 cut(s)	171, 1589, 2528, 2542, 2576
BspFNI	CGCG	2 cut(s)	2190, 3095
BspLI	GGNNCC	1 cut(s)	3053
BspMAI	CTGCAG	1 cut(s)	622
BspMI	ACCTGC	1 cut(s)	1091
BspPI	GGATC	3 cut(s)	894, 904, 3064
BspQI	GCTCTTC	1 cut(s)	177
BspT104I	TTCGAA	1 cut(s)	2150
BsrBI	CCGCTC	1 cut(s)	1265
BsrDI	GCAATG	2 cut(s)	1089, 2961
BsrGI	TGTACA	1 cut(s)	705
BsrI	ACTGG	5 cut(s)	418, 1146, 1860, 2164, 2605
BssECI	CCNNGG	4 cut(s)	488, 1182, 1762, 3073
BssMI	GATC	9 cut(s)	16, 886, 896, 1405, 1666, 1729, 1755, 2836, 3069
BssSI	CACGAG	1 cut(s)	924
Bst2BI	CACGAG	1 cut(s)	924
Bst2UI	CCWGG	7 cut(s)	1313, 1763, 1864, 1955, 2381, 2407, 3075
Bst6I	CTCTTC	1 cut(s)	177
BstAPI	GCANNNNNTGC	2 cut(s)	530, 2450
BstAUI	TGTACA	1 cut(s)	705
BstBI	TTCGAA	1 cut(s)	2150
BstC8I	GCNNGC	6 cut(s)	167, 339, 968, 1389, 2129, 2937
BstDEI	CTNAG	9 cut(s)	158, 1133, 1576, 1793, 1971, 2340, 2515, 2529, 2563
BstDSI	CCRYGG	1 cut(s)	488
BstFNI	CGCG	2 cut(s)	2190, 3095
BstH2I	RGCGCY	1 cut(s)	599
BstHHI	GCGC	5 cut(s)	93, 598, 786, 1614, 2192
BstKTI	GATC	9 cut(s)	19, 889, 899, 1408, 1669, 1732, 1758, 2839, 3072
BstMBI	GATC	9 cut(s)	16, 886, 896, 1405, 1666, 1729, 1755, 2836, 3069
BstMWI	GCNNNNNNNGC	8 cut(s)	97, 530, 792, 944, 1157, 2077, 2450, 2609
BstNI	CCWGG	7 cut(s)	1313, 1763, 1864, 1955, 2381, 2407, 3075
BstNSI	RCATGY	5 cut(s)	1814, 1930, 2300, 2434, 2939
BstSCI	CCNGG	7 cut(s)	1311, 1761, 1862, 1953, 2379, 2405, 3073
BstSFI	CTRYAG	2 cut(s)	538, 618
BstSLI	GKGCMC	1 cut(s)	925
BstUI	CGCG	2 cut(s)	2190, 3095
BstV1I	GCAGC	6 cut(s)	798, 1200, 1856, 1917, 2397, 2626
BstV2I	GAAGAC	2 cut(s)	2403, 3027
BstX2I	RGATCY	2 cut(s)	886, 3069
BstYI	RGATCY	2 cut(s)	886, 3069
BsuI	GTATCC	1 cut(s)	223
BsuRI	GGCC	5 cut(s)	169, 307, 687, 981, 2156
BtgI	CCRYGG	1 cut(s)	488
BtrI	CACGTC	1 cut(s)	935
BtsI	GCAGTG	1 cut(s)	1621
BtsIMutI	CAGTG	9 cut(s)	240, 384, 715, 1139, 1621, 2157, 2597, 2641, 2743
BveI	ACCTGC	1 cut(s)	1091
Cac8I	GCNNGC	6 cut(s)	167, 339, 968, 1389, 2129, 2937
CaiI	CAGNNNCTG	3 cut(s)	1435, 1855, 1994
CfoI	GCGC	5 cut(s)	93, 598, 786, 1614, 2192
Cfr13I	GGNCC	4 cut(s)	305, 979, 1957, 3077
Csp6I	GTAC	5 cut(s)	106, 706, 759, 1853, 2852
CviQI	GTAC	5 cut(s)	106, 706, 759, 1853, 2852
DdeI	CTNAG	9 cut(s)	158, 1133, 1576, 1793, 1971, 2340, 2515, 2529, 2563
DpnI	GATC	9 cut(s)	18, 888, 898, 1407, 1668, 1731, 1757, 2838, 3071
DpnII	GATC	9 cut(s)	16, 886, 896, 1405, 1666, 1729, 1755, 2836, 3069
DriI	GACNNNNNGTC	1 cut(s)	2758
Eam1104I	CTCTTC	1 cut(s)	177
Eam1105I	GACNNNNNGTC	1 cut(s)	2758
EarI	CTCTTC	1 cut(s)	177
Ecl136II	GAGCTC	1 cut(s)	1453
Eco147I	AGGCCT	2 cut(s)	169, 687
Eco24I	GRGCYC	1 cut(s)	1455
Eco47I	GGWCC	2 cut(s)	1957, 3077
Eco53kI	GAGCTC	1 cut(s)	1453
Eco57I	CTGAAG	1 cut(s)	492
Eco88I	CYCGRG	2 cut(s)	368, 1181
EcoICRI	GAGCTC	1 cut(s)	1453
EcoO109I	RGGNCCY	1 cut(s)	3077
EcoRI	GAATTC	1 cut(s)	1645
EcoRII	CCWGG	7 cut(s)	1311, 1761, 1862, 1953, 2379, 2405, 3073
EcoT22I	ATGCAT	2 cut(s)	1121, 1802
EcoT38I	GRGCYC	1 cut(s)	1455
FalI	AAGNNNNNCTT	6 cut(s)	1045, 1077, 2333, 2365, 2408, 2440
FaqI	GGGAC	4 cut(s)	63, 945, 1043, 2954
FauI	CCCGC	1 cut(s)	431
FbaI	TGATCA	2 cut(s)	16, 2836
Fnu4HI	GCNGC	8 cut(s)	89, 787, 1214, 1266, 1845, 1931, 2411, 2640
FokI	GGATG	9 cut(s)	37, 49, 454, 671, 1136, 1328, 1745, 2363, 2462
FriOI	GRGCYC	1 cut(s)	1455
Fsp4HI	GCNGC	8 cut(s)	89, 787, 1214, 1266, 1845, 1931, 2411, 2640
FspBI	CTAG	6 cut(s)	24, 593, 998, 1335, 2454, 3081
FspI	TGCGCA	1 cut(s)	1613
GlaI	GCGC	5 cut(s)	92, 597, 785, 1613, 2191
GluI	GCNGC	8 cut(s)	89, 787, 1214, 1266, 1845, 1931, 2411, 2640
GsaI	CCCAGC	2 cut(s)	980, 2976
GsuI	CTGGAG	1 cut(s)	1917
HaeII	RGCGCY	1 cut(s)	599
HaeIII	GGCC	5 cut(s)	169, 307, 687, 981, 2156
HhaI	GCGC	5 cut(s)	93, 598, 786, 1614, 2192
Hin6I	GCGC	5 cut(s)	91, 596, 784, 1612, 2190
HinP1I	GCGC	5 cut(s)	91, 596, 784, 1612, 2190
HincII	GTYRAC	2 cut(s)	2239, 3103
HindII	GTYRAC	2 cut(s)	2239, 3103
HindIII	AAGCTT	3 cut(s)	1037, 2125, 2277
HphI	GGTGA	4 cut(s)	39, 857, 2260, 2879
Hpy166II	GTNNAC	5 cut(s)	285, 706, 923, 2239, 3103
Hpy8I	GTNNAC	5 cut(s)	285, 706, 923, 2239, 3103
Hpy99I	CGWCG	1 cut(s)	3011
HpyAV	CCTTC	6 cut(s)	199, 1540, 1906, 2146, 2656, 3056
HpyCH4IV	ACGT	2 cut(s)	554, 934
HpyF10VI	GCNNNNNNNGC	8 cut(s)	97, 530, 792, 944, 1157, 2077, 2450, 2609
HpyF3I	CTNAG	9 cut(s)	158, 1133, 1576, 1793, 1971, 2340, 2515, 2529, 2563
HpySE526I	ACGT	2 cut(s)	554, 934
HspAI	GCGC	5 cut(s)	91, 596, 784, 1612, 2190
Ksp22I	TGATCA	2 cut(s)	16, 2836
Kzo9I	GATC	9 cut(s)	16, 886, 896, 1405, 1666, 1729, 1755, 2836, 3069
LguI	GCTCTTC	1 cut(s)	177
LmnI	GCTCC	5 cut(s)	853, 1549, 1915, 3008, 3051
Lsp1109I	GCAGC	6 cut(s)	798, 1200, 1856, 1917, 2397, 2626
MaeI	CTAG	6 cut(s)	24, 593, 998, 1335, 2454, 3081
MaeII	ACGT	2 cut(s)	554, 934
MaeIII	GTNAC	7 cut(s)	45, 238, 550, 1484, 1900, 2162, 2576
MalI	GATC	9 cut(s)	18, 888, 898, 1407, 1668, 1731, 1757, 2838, 3071
MbiI	CCGCTC	1 cut(s)	1265
MboI	GATC	9 cut(s)	16, 886, 896, 1405, 1666, 1729, 1755, 2836, 3069
MfeI	CAATTG	2 cut(s)	1355, 2243
MflI	RGATCY	2 cut(s)	886, 3069
MhlI	GDGCHC	3 cut(s)	925, 1455, 2564
MlyI	GAGTC	3 cut(s)	616, 2244, 2807
MmeI	TCCRAC	2 cut(s)	1130, 2775
Mph1103I	ATGCAT	2 cut(s)	1121, 1802
MroXI	GAANNNNTTC	3 cut(s)	1257, 1398, 1812
MslI	CAYNNNNRTG	4 cut(s)	245, 2761, 2868, 2940
MspR9I	CCNGG	7 cut(s)	1313, 1763, 1864, 1955, 2381, 2407, 3075
MunI	CAATTG	2 cut(s)	1355, 2243
MvaI	CCWGG	7 cut(s)	1313, 1763, 1864, 1955, 2381, 2407, 3075
MvnI	CGCG	2 cut(s)	2190, 3095
MwoI	GCNNNNNNNGC	8 cut(s)	97, 530, 792, 944, 1157, 2077, 2450, 2609
NdeII	GATC	9 cut(s)	16, 886, 896, 1405, 1666, 1729, 1755, 2836, 3069
NlaIV	GGNNCC	1 cut(s)	3053
NmuCI	GTSAC	3 cut(s)	45, 238, 550
NsbI	TGCGCA	1 cut(s)	1613
NsiI	ATGCAT	2 cut(s)	1121, 1802
NspI	RCATGY	5 cut(s)	1814, 1930, 2300, 2434, 2939
NspV	TTCGAA	1 cut(s)	2150
PaeI	GCATGC	1 cut(s)	2939
PaeR7I	CTCGAG	1 cut(s)	368
PceI	AGGCCT	2 cut(s)	169, 687
PciI	ACATGT	1 cut(s)	1810
PciSI	GCTCTTC	1 cut(s)	177
PcsI	WCGNNNNNNNCGW	2 cut(s)	496, 931
PdmI	GAANNNNTTC	3 cut(s)	1257, 1398, 1812
PfeI	GAWTC	8 cut(s)	375, 1058, 1859, 2525, 2651, 2726, 2774, 2981
PflMI	CCANNNNNTGG	1 cut(s)	208
PfoI	TCCNGGA	3 cut(s)	1311, 1862, 2379
PkrI	GCNGC	8 cut(s)	90, 788, 1215, 1267, 1846, 1932, 2412, 2641
PleI	GAGTC	3 cut(s)	615, 2243, 2807
PpsI	GAGTC	3 cut(s)	615, 2243, 2807
PpuMI	RGGWCCY	1 cut(s)	3077
PscI	ACATGT	1 cut(s)	1810
PshBI	ATTAAT	1 cut(s)	876
Psp124BI	GAGCTC	1 cut(s)	1455
Psp5II	RGGWCCY	1 cut(s)	3077
Psp6I	CCWGG	7 cut(s)	1311, 1761, 1862, 1953, 2379, 2405, 3073
PspFI	CCCAGC	2 cut(s)	976, 2972
PspGI	CCWGG	7 cut(s)	1311, 1761, 1862, 1953, 2379, 2405, 3073
PspN4I	GGNNCC	1 cut(s)	3053
PspPI	GGNCC	4 cut(s)	305, 979, 1957, 3077
PspPPI	RGGWCCY	1 cut(s)	3077
PstI	CTGCAG	1 cut(s)	622
PstNI	CAGNNNCTG	3 cut(s)	1435, 1855, 1994
PsuI	RGATCY	2 cut(s)	886, 3069
RsaI	GTAC	5 cut(s)	107, 707, 760, 1854, 2853
RsaNI	GTAC	5 cut(s)	106, 706, 759, 1853, 2852
RseI	CAYNNNNRTG	4 cut(s)	245, 2761, 2868, 2940
SacI	GAGCTC	1 cut(s)	1455
SapI	GCTCTTC	1 cut(s)	177
SatI	GCNGC	8 cut(s)	89, 787, 1214, 1266, 1845, 1931, 2411, 2640
Sau3AI	GATC	9 cut(s)	16, 886, 896, 1405, 1666, 1729, 1755, 2836, 3069
Sau96I	GGNCC	4 cut(s)	305, 979, 1957, 3077
SchI	GAGTC	3 cut(s)	616, 2244, 2807
ScrFI	CCNGG	7 cut(s)	1313, 1763, 1864, 1955, 2381, 2407, 3075
SduI	GDGCHC	3 cut(s)	925, 1455, 2564
SfcI	CTRYAG	2 cut(s)	538, 618
Sfr274I	CTCGAG	1 cut(s)	368
SfuI	TTCGAA	1 cut(s)	2150
SinI	GGWCC	2 cut(s)	1957, 3077
SlaI	CTCGAG	1 cut(s)	368
SmiMI	CAYNNNNRTG	4 cut(s)	245, 2761, 2868, 2940
SmlI	CTYRAG	4 cut(s)	101, 368, 1217, 1454
SmoI	CTYRAG	4 cut(s)	101, 368, 1217, 1454
SpeI	ACTAGT	1 cut(s)	997
SphI	GCATGC	1 cut(s)	2939
SseBI	AGGCCT	2 cut(s)	169, 687
SspI	AATATT	1 cut(s)	2474
SspMI	CTAG	6 cut(s)	24, 593, 998, 1335, 2454, 3081
SstI	GAGCTC	1 cut(s)	1455
StuI	AGGCCT	2 cut(s)	169, 687
StyD4I	CCNGG	7 cut(s)	1311, 1761, 1862, 1953, 2379, 2405, 3073
TaiI	ACGT	2 cut(s)	557, 937
TaqI	TCGA	6 cut(s)	369, 1261, 2150, 2649, 2988, 3009
TatI	WGTACW	3 cut(s)	105, 705, 2851
TauI	GCSGC	2 cut(s)	91, 1268
TfiI	GAWTC	8 cut(s)	375, 1058, 1859, 2525, 2651, 2726, 2774, 2981
TscAI	CASTG	9 cut(s)	247, 391, 715, 1146, 1621, 2164, 2597, 2641, 2743
TseFI	GTSAC	3 cut(s)	45, 238, 550
TseI	GCWGC	6 cut(s)	786, 1213, 1844, 1930, 2410, 2639
Tsp45I	GTSAC	3 cut(s)	45, 238, 550
TspGWI	ACGGA	3 cut(s)	2474, 2995, 3064
TspRI	CASTG	9 cut(s)	247, 391, 715, 1146, 1621, 2164, 2597, 2641, 2743
Van91I	CCANNNNNTGG	1 cut(s)	208
VneI	GTGCAC	1 cut(s)	921
VpaK11BI	GGWCC	2 cut(s)	1957, 3077
VspI	ATTAAT	1 cut(s)	876
XapI	RAATTY	5 cut(s)	504, 720, 1645, 2226, 2251
XceI	RCATGY	5 cut(s)	1814, 1930, 2300, 2434, 2939
XhoI	CTCGAG	1 cut(s)	368
XmnI	GAANNNNTTC	3 cut(s)	1257, 1398, 1812
XspI	CTAG	6 cut(s)	24, 593, 998, 1335, 2454, 3081
Zsp2I	ATGCAT	2 cut(s)	1121, 1802

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.