Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009486

Physical Location & Seq

Genomic Coordinates Forward (+)

21394534 .. 21400815

Gene Structure 6282 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1136800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3120 bp

ATGGCACTCACCCCTGATCACACCCCTGATCACACTAGGAAAGTTGGGATTGGGGTGTCACCATCCCCTTCTCCTTTTCTTACTCCTCGTCCCGAAAGGCGGCGGAAAACTCTTGAGTACAATCCAAATCGACAGGACAAGGATAAAGAGGTTAACGTGCAAGTTTTGCTGAGATGCAGGCCATTAAGTGATGAAGAGCAAAGGTTGAATGTCCAGAAGGTGGTATCCTGTAATGAACAGAAAAGAGAGGTCACTGTTATGCAAAGTCTAAATAATAAGCAAGTCGATAAATTGTTCACTTTTGACAGGGTTTTTGGACCAAAAGCGCAGCAAAAATCAATATATGAGCAGGCCATTTCCCCGATAGTTAATGAAGCTCTTGACGGATTCAACTGCACTGTCTTCGCTTATGGGCAGACAGGCACTGGTAAAACTTATACAATGGAAGGTGGGATGAGAAATAAGAGTGGCAATTTGCCTACTGAGGCTGGTGTTATTCCACGGGCTATTCGTCAAATTTTTGATACACTTGAAGCACAAGATGCTGACTACAGTGTGAAAGTAACGTTTTTGGAAATATATAATGAAGAAATAACTGATTTACTAGCTCCTGATGAGAATCCAAGAACTGCGGACGACAGGCAAAAGAAATCTATTTCGTTGATGGAGGATGGGAAGGGTTGTGTAATAGTAAGAGGCCTTGAAGAAGAATATGTGTATAGCGTAAATGAAATTTATAGTCTCTTGGAACGAGGGTCTTCCAAAAGGCGTACAGCAGACACTCTGATGAACAAGCGCAGCAGTCGCTCTCATTCTGTCTTTTCCATTACTGTCCACATAAAAGAAGCAACAGTTGGTGATGAGGAGCTAATTAAATGTGGCAAAATTAATCTAGTGGATCTGGCAGGATCGGAAAACATATCTCGTTCAGGTGCACGAGAGGGACGTGCAAGGGAAGCAGGGGAAATAAATAAGAGCTTGCTCACGCTGGGCCGCGTCATAAATGCACTAGTGGAACATTCCACTCATATACCTTACAGGGACAGTAAGCTTACAAGACTTTTAAGAGATTCTTTGGGAGGGAAAACAAAAACCTGCATCATTGCCACAATTTCTCCAACTGCTTCATGCATGGAGGAAACTCTAAGCACACTGGATTATGCCTATCGTGCCAAGAACATTAAAAACAAACCCGAGGCAAACCAGAAAATGTCAAAGCCTGTGCTGCTCAAGGAATTGTATTCAGAGATTGAGAGAATGAAAGAAGATATCCGAGCGGCACGGGAAAAGAATGGTGTTTATATTCCGCATGAAAGATTTGTCCAGGATGAAGCTGAAAAAAAGGCTAGGATTGAGAGAATAGAGCAATTGGAGAATGACCTCAACCTCACAGAAAAGCAAGCGGATAGTTTTCATGAGCTCTATATCAGTGAACAAGAACAGAAGCTGGATTTACAAAGTGAGCTCAAGGACTGCAAGATAAATCTGGATAACTGTAACAAGGCTTTGCTTGAACTTCAAGAGGATTACCAAATAGCCACCTCAACCCTGAAAGAGAAGGAGCGTATCATCTCCAAATTGCTATTCTCAGAAAATCTTTTGATTGGACGGGCAAAGGAGTTGCGCAGTGATTTGAAGAATGCATCAGAGGAAATGAATTCCCTCTATGAAAAATTAGATAAGAAAGACAAGATGGAAGCAGAAAATCAGAGCTTGGTTATGGCATTTGGTTCTCAGCTTGATCGGAGTTTGAAAGACCTGCATAAGACCATCCTAGGGTCTGTTTCTCAACAGCAGTATCAACTAAGATGCATGGAGGAACATGTTCAGTCATACCTTGCGAGTAAATGTGATGCAGCACAGGTGCTGGAATCCAGGATTAAGAAGATAACAGAGACATATAGTTCTGGAGTAACAGCCTTGAAGGAGCTGGCCAACATGCTGCAAAAGAAAGCATCATCTGACCTGGAGCAAATAAATGCTAAGACGTTATCACAGACAGTGGCTGTTGCGAAGTTTCTTGAAACTGCGGTTAAGGAAGCCAATGAGGTCATCCAAGATATTCAGCACTCTCTTGATGAGCAGAAACAGCTATTGGCTTTCTCCGCTCAACAACAAGAGGAGGGATTGCAAAGGAGCTTGATGTCAGCACAAGTAATTTCGAAGGCCACTGGGAACTTTTTTGATGACCTCCAGGACCGCACTTGTCAAGTCATGAGAAATCTTGAAGAAACCCAAATTCAACGAGTCAACCAATTGGTGAATTTTGAGAAAATGTTTAAGGAAGAAGCTTCTAGAGAGGAAAAACAGGCTATGGAAAAAATTGCAATAATAATTGGAACATTGACATCTAAGAAAGCCTCTATGGTATCGGAGGCCTCAACCGACATCCAGGAAAGGAGTAAAGAAGAGAACACCAGGTTGCAAAAGGCAATGTTTAACATGCAAAAAGTGGCAACGGATGCTAGGAAGGAGTTGAGTGAATATTTCAGAAAGGTGGAATGTGATTTTATGAAAGACACATTCTCAGCAGTTGAATCTCAGGCAATCATGGAAAACTGCCTTCAAGAGTGCTCAGAGAGAGTGGGTTACTCTAGCAAACAGTGGGAAAATGCCCAGTCAGCCATAAATACTCTTAATCAGAACAGTGCTGCAAATATCGAATCTACTGTGAAGGAAAATATCTCTGCTAATCATTCTGCACATGAAAAGTTTGTTTCCGCATCTTCCCGTGTGGATTCAGATTTCAATGCTTTAGCCACCGACACATTGTCATGCGTTAATGATTCGCTAATGCTGGACTATGAAAAGACAAAGGAAATAGACTCCGTGACTGCTGTGTGTGTGGATCAACTCAAATCAGTACAAGAGAAGCATGGTGATGGTGTATCAACTATACGAAATCAAGCAGAAAAGTGCCTTGTACAAGATTATTTGGTGGATAAGCATGCCGGTGCAACCAAGAAACAAGTCATAGCTGTCCCCAGCTTGGAATCTATCGAGGAGATGAGAAGCTCCTTCGATGTCAAAGAAGACGGTACATCTGATAAAAAATTAAAACGGAGCCAAATGGAAATCAAGATCCCGGGTCCTAGAACTCCTTTCGCAGATGTCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0008574 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1040

Amino Acids

117.42

Weight (kDa)

5.73

Isoelectric Point (pI)

47.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	48 - 202	5.2e-28	Microtubule binding
Kinesin	PF00225	58 - 394	1.1e-118	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009486)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37420 AT2G37420
fragaria_vesca	FvH4_6g14420
malus_domestica	MD04G1119500.v1.1 MD12G1136800.v1.1
prunus_persica	Prupe.6G249200_v2.0.a1
pyrus_communis	pycom04g10890 pycom04g10900 pycom12g13130
rosa_chinensis	RchiOBHm_Chr3g0467551
rosa_laevigata	RLG00000024492
rosa_multiflora	Rmu_sc0000286.1_g000001 Rmu_sc0027582.1_g000001
rosa_roxburghii	Rroxscaffold_6G00413600
rosa_rugosa	Rorug03G0087900 Rorug03G0088000 Rorug03G0088100
rosa_samantha	Rh3AG135900 Rh3BG157700 Rh3CG157600 Rh3DG158500
rosa_wichuraiana	Rw3G012910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1625
Acc36I	ACCTGC	2 cut(s)	1103, 1767
AccB7I	CCANNNNNTGG	1 cut(s)	220
AccBSI	CCGCTC	2 cut(s)	1277, 2108
AccII	CGCG	1 cut(s)	996
AclI	AACGTT	1 cut(s)	566
AclWI	GGATC	4 cut(s)	906, 916, 2856, 3076
AcoI	YGGCCR	1 cut(s)	1932
AcsI	RAATTY	5 cut(s)	516, 732, 1657, 2238, 2263
AfaI	GTAC	5 cut(s)	119, 772, 2865, 2925, 3040
AfiI	CCNNNNNNNGG	4 cut(s)	99, 220, 1776, 2989
AflIII	ACRYGT	1 cut(s)	1822
AhdI	GACNNNNNGTC	1 cut(s)	2770
AhlI	ACTAGT	1 cut(s)	1009
AjiI	CACGTC	1 cut(s)	947
AjnI	CCWGG	6 cut(s)	1323, 1874, 1965, 2193, 2391, 2417
AjuI	GAANNNNNNNTTGG	6 cut(s)	837, 869, 1568, 1600, 2078, 2110
Alw21I	GWGCWC	4 cut(s)	937, 1422, 1467, 2576
Alw26I	GTCTC	2 cut(s)	746, 1889
Alw44I	GTGCAC	1 cut(s)	933
AlwI	GGATC	4 cut(s)	906, 916, 2856, 3076
AlwNI	CAGNNNCTG	4 cut(s)	425, 1447, 1867, 2006
Ama87I	CYCGRG	2 cut(s)	1193, 3085
AoxI	GGCC	7 cut(s)	179, 351, 697, 991, 1932, 2166, 2376
ApaLI	GTGCAC	1 cut(s)	933
ApeKI	GCWGC	6 cut(s)	328, 798, 1225, 1856, 1942, 2651
ApoI	RAATTY	5 cut(s)	516, 732, 1657, 2238, 2263
ArsI	GACNNNNNNTTYG	2 cut(s)	296, 328
AseI	ATTAAT	1 cut(s)	888
Asp700I	GAANNNNTTC	1 cut(s)	1824
AspA2I	CCTAGG	1 cut(s)	1774
AspLEI	GCGC	3 cut(s)	328, 798, 1626
AspS9I	GGNCC	4 cut(s)	317, 991, 2197, 3089
AsuC2I	CCSGG	2 cut(s)	3086, 3087
AsuHPI	GGTGA	4 cut(s)	51, 869, 2272, 2891
AsuII	TTCGAA	1 cut(s)	2162
AvaI	CYCGRG	2 cut(s)	1193, 3085
AvaII	GGWCC	3 cut(s)	317, 2197, 3089
AvrII	CCTAGG	1 cut(s)	1774
BaeGI	GKGCMC	1 cut(s)	937
BaeI	ACNNNNGTAYC	2 cut(s)	1781, 1814
BalI	TGGCCA	1 cut(s)	1934
BanII	GRGCYC	2 cut(s)	1422, 1467
BauI	CACGAG	1 cut(s)	936
BbsI	GAAGAC	3 cut(s)	394, 750, 3039
Bbv12I	GWGCWC	4 cut(s)	937, 1422, 1467, 2576
BbvI	GCAGC	6 cut(s)	340, 810, 1212, 1868, 1929, 2638
BccI	CCATC	6 cut(s)	70, 658, 665, 1687, 1778, 2876
BcgI	CGANNNNNNTGC	2 cut(s)	385, 419
BciT130I	CCWGG	6 cut(s)	1325, 1876, 1967, 2195, 2393, 2419
BciVI	GTATCC	1 cut(s)	235
BclI	TGATCA	2 cut(s)	16, 28
BcnI	CCSGG	2 cut(s)	3086, 3087
BcoDI	GTCTC	2 cut(s)	746, 1889
BcuI	ACTAGT	1 cut(s)	1009
BfmI	CTRYAG	1 cut(s)	550
BfuAI	ACCTGC	2 cut(s)	1103, 1767
BfuI	GTATCC	1 cut(s)	235
BisI	GCNGC	9 cut(s)	101, 329, 799, 994, 1226, 1278, 1857, 1943, 2652
BlnI	CCTAGG	1 cut(s)	1774
BlsI	GCNGC	9 cut(s)	102, 330, 800, 995, 1227, 1279, 1858, 1944, 2653
Bme1390I	CCNGG	8 cut(s)	1325, 1876, 1967, 2195, 2393, 2419, 3086, 3087
Bme18I	GGWCC	3 cut(s)	317, 2197, 3089
BmeRI	GACNNNNNGTC	1 cut(s)	2770
BmeT110I	CYCGRG	2 cut(s)	1193, 3085
BmgBI	CACGTC	1 cut(s)	947
BmgT120I	GGNCC	4 cut(s)	317, 991, 2197, 3089
BmiI	GGNNCC	2 cut(s)	3065, 3090
BmrFI	CCNGG	8 cut(s)	1325, 1876, 1967, 2195, 2393, 2419, 3086, 3087
BmrI	ACTGGG	2 cut(s)	2181, 2611
BmsI	GCATC	9 cut(s)	164, 532, 1107, 1652, 1799, 1843, 1964, 2452, 2732
BmuI	ACTGGG	2 cut(s)	2181, 2611
BpiI	GAAGAC	3 cut(s)	394, 750, 3039
BpmI	CTGGAG	3 cut(s)	1929, 1988, 2177
Bpu14I	TTCGAA	1 cut(s)	2162
BpuEI	CTTGAG	3 cut(s)	134, 1214, 1451
BpuMI	CCSGG	2 cut(s)	3086, 3087
BsaBI	GATNNNNATC	1 cut(s)	618
BsaJI	CCNNGG	4 cut(s)	500, 1194, 1774, 3085
BsaXI	ACNNNNNCTCC	2 cut(s)	1099, 1129
Bsc4I	CCNNNNNNNGG	4 cut(s)	99, 220, 1776, 2989
Bse118I	RCCGGY	1 cut(s)	2951
Bse1I	ACTGG	4 cut(s)	430, 1158, 2176, 2617
Bse3DI	GCAATG	2 cut(s)	1101, 2439
Bse8I	GATNNNNATC	1 cut(s)	618
BseBI	CCWGG	6 cut(s)	1325, 1876, 1967, 2195, 2393, 2419
BseDI	CCNNGG	4 cut(s)	500, 1194, 1774, 3085
BseGI	GGATG	8 cut(s)	62, 459, 676, 1333, 1770, 2052, 2388, 2467
BseJI	GATNNNNATC	1 cut(s)	618
BseLI	CCNNNNNNNGG	4 cut(s)	99, 220, 1776, 2989
BseMI	GCAATG	2 cut(s)	1101, 2439
BseMII	CTCAG	7 cut(s)	161, 474, 1602, 1748, 2541, 2555, 2589
BseNI	ACTGG	4 cut(s)	430, 1158, 2176, 2617
BseRI	GAGGAG	4 cut(s)	75, 878, 2135, 3017
BseSI	GKGCMC	1 cut(s)	937
BseXI	GCAGC	6 cut(s)	340, 810, 1212, 1868, 1929, 2638
BseYI	CCCAGC	2 cut(s)	988, 2984
BsgI	GTGCAG	2 cut(s)	379, 2685
Bsh1236I	CGCG	1 cut(s)	996
BshFI	GGCC	7 cut(s)	181, 353, 699, 993, 1934, 2168, 2378
BsiHKAI	GWGCWC	4 cut(s)	937, 1422, 1467, 2576
BsiHKCI	CYCGRG	2 cut(s)	1193, 3085
BsiSI	CCGG	2 cut(s)	2952, 3086
BslFI	GGGAC	4 cut(s)	75, 957, 1055, 2966
BslI	CCNNNNNNNGG	4 cut(s)	99, 220, 1776, 2989
BsmAI	GTCTC	2 cut(s)	746, 1889
BsmFI	GGGAC	4 cut(s)	75, 957, 1055, 2966
BsmI	GAATGC	1 cut(s)	1645
BsnI	GGCC	7 cut(s)	181, 353, 699, 993, 1934, 2168, 2378
BsoBI	CYCGRG	2 cut(s)	1193, 3085
Bsp119I	TTCGAA	1 cut(s)	2162
Bsp1286I	GDGCHC	4 cut(s)	937, 1422, 1467, 2576
Bsp1407I	TGTACA	1 cut(s)	2923
Bsp143I	GATC	7 cut(s)	16, 28, 898, 908, 1741, 2848, 3081
BspANI	GGCC	7 cut(s)	181, 353, 699, 993, 1934, 2168, 2378
BspCNI	CTCAG	7 cut(s)	162, 475, 1601, 1747, 2540, 2554, 2588
BspFNI	CGCG	1 cut(s)	996
BspHI	TCATGA	2 cut(s)	1414, 2214
BspLI	GGNNCC	2 cut(s)	3065, 3090
BspMI	ACCTGC	2 cut(s)	1103, 1767
BspPI	GGATC	4 cut(s)	906, 916, 2856, 3076
BspQI	GCTCTTC	1 cut(s)	189
BspT104I	TTCGAA	1 cut(s)	2162
BsrBI	CCGCTC	2 cut(s)	1277, 2108
BsrDI	GCAATG	2 cut(s)	1101, 2439
BsrFI	RCCGGY	1 cut(s)	2951
BsrGI	TGTACA	1 cut(s)	2923
BsrI	ACTGG	4 cut(s)	430, 1158, 2176, 2617
BssAI	RCCGGY	1 cut(s)	2951
BssECI	CCNNGG	4 cut(s)	500, 1194, 1774, 3085
BssMI	GATC	7 cut(s)	16, 28, 898, 908, 1741, 2848, 3081
BssSI	CACGAG	1 cut(s)	936
BssT1I	CCWWGG	1 cut(s)	1774
Bst2BI	CACGAG	1 cut(s)	936
Bst2UI	CCWGG	6 cut(s)	1325, 1876, 1967, 2195, 2393, 2419
Bst6I	CTCTTC	2 cut(s)	189, 2403
BstAPI	GCANNNNNTGC	3 cut(s)	166, 542, 2462
BstAUI	TGTACA	1 cut(s)	2923
BstBI	TTCGAA	1 cut(s)	2162
BstC8I	GCNNGC	6 cut(s)	179, 351, 980, 1401, 1932, 2949
BstDSI	CCRYGG	1 cut(s)	500
BstF5I	GGATG	8 cut(s)	62, 459, 676, 1333, 1770, 2052, 2388, 2467
BstFNI	CGCG	1 cut(s)	996
BstHHI	GCGC	3 cut(s)	328, 798, 1626
BstKTI	GATC	7 cut(s)	19, 31, 901, 911, 1744, 2851, 3084
BstMAI	GTCTC	2 cut(s)	746, 1889
BstMBI	GATC	7 cut(s)	16, 28, 898, 908, 1741, 2848, 3081
BstNI	CCWGG	6 cut(s)	1325, 1876, 1967, 2195, 2393, 2419
BstNSI	RCATGY	4 cut(s)	1826, 1942, 2446, 2951
BstSCI	CCNGG	8 cut(s)	1323, 1874, 1965, 2193, 2391, 2417, 3084, 3085
BstSFI	CTRYAG	1 cut(s)	550
BstSLI	GKGCMC	1 cut(s)	937
BstUI	CGCG	1 cut(s)	996
BstV1I	GCAGC	6 cut(s)	340, 810, 1212, 1868, 1929, 2638
BstV2I	GAAGAC	3 cut(s)	394, 750, 3039
BstX2I	RGATCY	2 cut(s)	898, 3081
BstYI	RGATCY	2 cut(s)	898, 3081
BsuI	GTATCC	1 cut(s)	235
BsuRI	GGCC	7 cut(s)	181, 353, 699, 993, 1934, 2168, 2378
BtgI	CCRYGG	1 cut(s)	500
BtrI	CACGTC	1 cut(s)	947
BtsCI	GGATG	8 cut(s)	62, 459, 676, 1333, 1770, 2052, 2388, 2467
BtsI	GCAGTG	1 cut(s)	1633
BveI	ACCTGC	2 cut(s)	1103, 1767
Cac8I	GCNNGC	6 cut(s)	179, 351, 980, 1401, 1932, 2949
CaiI	CAGNNNCTG	4 cut(s)	425, 1447, 1867, 2006
CciI	TCATGA	2 cut(s)	1414, 2214
CfoI	GCGC	3 cut(s)	328, 798, 1626
Cfr10I	RCCGGY	1 cut(s)	2951
Cfr13I	GGNCC	4 cut(s)	317, 991, 2197, 3089
Cfr9I	CCCGGG	1 cut(s)	3085
CseI	GACGC	1 cut(s)	985
CsiI	ACCWGGT	1 cut(s)	2417
Csp6I	GTAC	5 cut(s)	118, 771, 2864, 2924, 3039
CviQI	GTAC	5 cut(s)	118, 771, 2864, 2924, 3039
DpnI	GATC	7 cut(s)	18, 30, 900, 910, 1743, 2850, 3083
DpnII	GATC	7 cut(s)	16, 28, 898, 908, 1741, 2848, 3081
DriI	GACNNNNNGTC	1 cut(s)	2770
EaeI	YGGCCR	1 cut(s)	1932
Eam1104I	CTCTTC	2 cut(s)	189, 2403
Eam1105I	GACNNNNNGTC	1 cut(s)	2770
EarI	CTCTTC	2 cut(s)	189, 2403
EciI	GGCGGA	1 cut(s)	118
Ecl136II	GAGCTC	2 cut(s)	1420, 1465
Eco130I	CCWWGG	1 cut(s)	1774
Eco147I	AGGCCT	2 cut(s)	699, 2378
Eco24I	GRGCYC	2 cut(s)	1422, 1467
Eco32I	GATATC	1 cut(s)	1270
Eco47I	GGWCC	3 cut(s)	317, 2197, 3089
Eco53kI	GAGCTC	2 cut(s)	1420, 1465
Eco88I	CYCGRG	2 cut(s)	1193, 3085
EcoICRI	GAGCTC	2 cut(s)	1420, 1465
EcoO109I	RGGNCCY	1 cut(s)	3089
EcoRI	GAATTC	1 cut(s)	1657
EcoRII	CCWGG	6 cut(s)	1323, 1874, 1965, 2193, 2391, 2417
EcoRV	GATATC	1 cut(s)	1270
EcoT14I	CCWWGG	1 cut(s)	1774
EcoT22I	ATGCAT	3 cut(s)	1133, 1645, 1814
EcoT38I	GRGCYC	2 cut(s)	1422, 1467
ErhI	CCWWGG	1 cut(s)	1774
FalI	AAGNNNNNCTT	2 cut(s)	1057, 1089
FaqI	GGGAC	4 cut(s)	75, 957, 1055, 2966
FbaI	TGATCA	2 cut(s)	16, 28
Fnu4HI	GCNGC	9 cut(s)	101, 329, 799, 994, 1226, 1278, 1857, 1943, 2652
FokI	GGATG	8 cut(s)	49, 466, 683, 1340, 1757, 2039, 2375, 2474
FriOI	GRGCYC	2 cut(s)	1422, 1467
Fsp4HI	GCNGC	9 cut(s)	101, 329, 799, 994, 1226, 1278, 1857, 1943, 2652
FspI	TGCGCA	1 cut(s)	1625
GlaI	GCGC	3 cut(s)	327, 797, 1625
GluI	GCNGC	9 cut(s)	101, 329, 799, 994, 1226, 1278, 1857, 1943, 2652
GsaI	CCCAGC	2 cut(s)	992, 2988
GsuI	CTGGAG	3 cut(s)	1929, 1988, 2177
HaeIII	GGCC	7 cut(s)	181, 353, 699, 993, 1934, 2168, 2378
HapII	CCGG	2 cut(s)	2952, 3086
HgaI	GACGC	1 cut(s)	985
HhaI	GCGC	3 cut(s)	328, 798, 1626
Hin6I	GCGC	3 cut(s)	326, 796, 1624
HinP1I	GCGC	3 cut(s)	326, 796, 1624
HincII	GTYRAC	3 cut(s)	154, 2251, 3115
HindII	GTYRAC	3 cut(s)	154, 2251, 3115
HindIII	AAGCTT	2 cut(s)	1049, 2289
HpaI	GTTAAC	1 cut(s)	154
HpaII	CCGG	2 cut(s)	2952, 3086
HphI	GGTGA	4 cut(s)	51, 869, 2272, 2891
Hpy166II	GTNNAC	7 cut(s)	154, 297, 835, 935, 1433, 2251, 3115
Hpy8I	GTNNAC	7 cut(s)	154, 297, 835, 935, 1433, 2251, 3115
HpyCH4IV	ACGT	4 cut(s)	156, 566, 946, 1988
HpySE526I	ACGT	4 cut(s)	156, 566, 946, 1988
HspAI	GCGC	3 cut(s)	326, 796, 1624
Ksp22I	TGATCA	2 cut(s)	16, 28
KspAI	GTTAAC	1 cut(s)	154
Kzo9I	GATC	7 cut(s)	16, 28, 898, 908, 1741, 2848, 3081
LguI	GCTCTTC	1 cut(s)	189
LmnI	GCTCC	8 cut(s)	613, 865, 1561, 1927, 1969, 2136, 3020, 3063
Lsp1109I	GCAGC	6 cut(s)	340, 810, 1212, 1868, 1929, 2638
LweI	GCATC	9 cut(s)	164, 532, 1107, 1652, 1799, 1843, 1964, 2452, 2732
MabI	ACCWGGT	1 cut(s)	2417
MaeII	ACGT	4 cut(s)	156, 566, 946, 1988
MaeIII	GTNAC	7 cut(s)	57, 250, 562, 1496, 1912, 2588, 2830
MalI	GATC	7 cut(s)	18, 30, 900, 910, 1743, 2850, 3083
MbiI	CCGCTC	2 cut(s)	1277, 2108
MboI	GATC	7 cut(s)	16, 28, 898, 908, 1741, 2848, 3081
MfeI	CAATTG	2 cut(s)	1367, 2255
MflI	RGATCY	2 cut(s)	898, 3081
MhlI	GDGCHC	4 cut(s)	937, 1422, 1467, 2576
MlsI	TGGCCA	1 cut(s)	1934
MluNI	TGGCCA	1 cut(s)	1934
MlyI	GAGTC	2 cut(s)	2256, 2819
MmeI	TCCRAC	1 cut(s)	1142
Mox20I	TGGCCA	1 cut(s)	1934
Mph1103I	ATGCAT	3 cut(s)	1133, 1645, 1814
MroXI	GAANNNNTTC	1 cut(s)	1824
MscI	TGGCCA	1 cut(s)	1934
MslI	CAYNNNNRTG	5 cut(s)	257, 785, 2773, 2880, 2952
Msp20I	TGGCCA	1 cut(s)	1934
MspI	CCGG	2 cut(s)	2952, 3086
MspR9I	CCNGG	8 cut(s)	1325, 1876, 1967, 2195, 2393, 2419, 3086, 3087
MunI	CAATTG	2 cut(s)	1367, 2255
Mva1269I	GAATGC	1 cut(s)	1645
MvaI	CCWGG	6 cut(s)	1325, 1876, 1967, 2195, 2393, 2419
MvnI	CGCG	1 cut(s)	996
NciI	CCSGG	2 cut(s)	3086, 3087
NdeII	GATC	7 cut(s)	16, 28, 898, 908, 1741, 2848, 3081
NlaIV	GGNNCC	2 cut(s)	3065, 3090
NmuCI	GTSAC	3 cut(s)	57, 250, 2830
NsbI	TGCGCA	1 cut(s)	1625
NsiI	ATGCAT	3 cut(s)	1133, 1645, 1814
NspI	RCATGY	4 cut(s)	1826, 1942, 2446, 2951
NspV	TTCGAA	1 cut(s)	2162
PaeI	GCATGC	1 cut(s)	2951
PagI	TCATGA	2 cut(s)	1414, 2214
PceI	AGGCCT	2 cut(s)	699, 2378
PciI	ACATGT	1 cut(s)	1822
PciSI	GCTCTTC	1 cut(s)	189
PcsI	WCGNNNNNNNCGW	2 cut(s)	508, 943
PctI	GAATGC	1 cut(s)	1645
PdmI	GAANNNNTTC	1 cut(s)	1824
PfeI	GAWTC	9 cut(s)	387, 619, 1070, 1871, 2537, 2663, 2738, 2786, 2993
PflMI	CCANNNNNTGG	1 cut(s)	220
PfoI	TCCNGGA	4 cut(s)	1323, 1874, 2193, 2391
PkrI	GCNGC	9 cut(s)	102, 330, 800, 995, 1227, 1279, 1858, 1944, 2653
PleI	GAGTC	2 cut(s)	2255, 2819
PpsI	GAGTC	2 cut(s)	2255, 2819
PpuMI	RGGWCCY	1 cut(s)	3089
PscI	ACATGT	1 cut(s)	1822
PshBI	ATTAAT	1 cut(s)	888
Psp124BI	GAGCTC	2 cut(s)	1422, 1467
Psp1406I	AACGTT	1 cut(s)	566
Psp5II	RGGWCCY	1 cut(s)	3089
Psp6I	CCWGG	6 cut(s)	1323, 1874, 1965, 2193, 2391, 2417
PspFI	CCCAGC	2 cut(s)	988, 2984
PspGI	CCWGG	6 cut(s)	1323, 1874, 1965, 2193, 2391, 2417
PspN4I	GGNNCC	2 cut(s)	3065, 3090
PspPI	GGNCC	4 cut(s)	317, 991, 2197, 3089
PspPPI	RGGWCCY	1 cut(s)	3089
PstNI	CAGNNNCTG	4 cut(s)	425, 1447, 1867, 2006
PsuI	RGATCY	2 cut(s)	898, 3081
RsaI	GTAC	5 cut(s)	119, 772, 2865, 2925, 3040
RsaNI	GTAC	5 cut(s)	118, 771, 2864, 2924, 3039
RseI	CAYNNNNRTG	5 cut(s)	257, 785, 2773, 2880, 2952
SacI	GAGCTC	2 cut(s)	1422, 1467
SapI	GCTCTTC	1 cut(s)	189
SatI	GCNGC	9 cut(s)	101, 329, 799, 994, 1226, 1278, 1857, 1943, 2652
Sau3AI	GATC	7 cut(s)	16, 28, 898, 908, 1741, 2848, 3081
Sau96I	GGNCC	4 cut(s)	317, 991, 2197, 3089
SchI	GAGTC	2 cut(s)	2256, 2819
ScrFI	CCNGG	8 cut(s)	1325, 1876, 1967, 2195, 2393, 2419, 3086, 3087
SduI	GDGCHC	4 cut(s)	937, 1422, 1467, 2576
SexAI	ACCWGGT	1 cut(s)	2417
SfaNI	GCATC	9 cut(s)	164, 532, 1107, 1652, 1799, 1843, 1964, 2452, 2732
SfcI	CTRYAG	1 cut(s)	550
SfuI	TTCGAA	1 cut(s)	2162
SinI	GGWCC	3 cut(s)	317, 2197, 3089
SmaI	CCCGGG	1 cut(s)	3087
SmiMI	CAYNNNNRTG	5 cut(s)	257, 785, 2773, 2880, 2952
SmlI	CTYRAG	3 cut(s)	113, 1229, 1466
SmoI	CTYRAG	3 cut(s)	113, 1229, 1466
SpeI	ACTAGT	1 cut(s)	1009
SphI	GCATGC	1 cut(s)	2951
SseBI	AGGCCT	2 cut(s)	699, 2378
SspI	AATATT	1 cut(s)	2486
SstI	GAGCTC	2 cut(s)	1422, 1467
StuI	AGGCCT	2 cut(s)	699, 2378
StyD4I	CCNGG	8 cut(s)	1323, 1874, 1965, 2193, 2391, 2417, 3084, 3085
StyI	CCWWGG	1 cut(s)	1774
TaiI	ACGT	4 cut(s)	159, 569, 949, 1991
TaqI	TCGA	6 cut(s)	130, 285, 2162, 2661, 3000, 3021
TatI	WGTACW	3 cut(s)	117, 2863, 2923
TauI	GCSGC	3 cut(s)	103, 996, 1280
TfiI	GAWTC	9 cut(s)	387, 619, 1070, 1871, 2537, 2663, 2738, 2786, 2993
TseFI	GTSAC	3 cut(s)	57, 250, 2830
TseI	GCWGC	6 cut(s)	328, 798, 1225, 1856, 1942, 2651
Tsp45I	GTSAC	3 cut(s)	57, 250, 2830
TspGWI	ACGGA	4 cut(s)	399, 2474, 2818, 3076
TspMI	CCCGGG	1 cut(s)	3085
Van91I	CCANNNNNTGG	1 cut(s)	220
VneI	GTGCAC	1 cut(s)	933
VpaK11BI	GGWCC	3 cut(s)	317, 2197, 3089
VspI	ATTAAT	1 cut(s)	888
XapI	RAATTY	5 cut(s)	516, 732, 1657, 2238, 2263
XbaI	TCTAGA	1 cut(s)	2294
XceI	RCATGY	4 cut(s)	1826, 1942, 2446, 2951
XmaI	CCCGGG	1 cut(s)	3085
XmaJI	CCTAGG	1 cut(s)	1774
XmnI	GAANNNNTTC	1 cut(s)	1824
Zsp2I	ATGCAT	3 cut(s)	1133, 1645, 1814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.