Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005633

Physical Location & Seq

Genomic Coordinates Forward (+)

21083975 .. 21087185

Gene Structure 3211 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1124300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1473 bp

ATGGCGGCATCTCGTCGGCTCCGGGACCTGCAATCGCAGCCGGGGAACAAGATCTGCGTGGACTGCTCCCAGAAGAACCCGCAATGGGCCTCGGTATCCTACGGTGTCTTCATGTGCTTAGAGTGCTCCGGCAAGCACCGCGGGCTCGGCGTCCACATCTCGTTCGTCCGATCCGTGACAATGGACTCGTGGTCCGAGATCCAGATCAAGAAGATGGAGGCTGGTGGCAACGAGAAGCTCAACGCCTTCCTAGCCAGCTACGGCGTTCCTAAGGAGACGGATATCGTCACCAAGTACAATACCAACTCTGCCAGCGTGTACCGGGATCGGATTCAAGCCCTAGCCGAGGGGCGTCCCTGGAGGGACCCACCTGTTGTTCAGGAGACCATAGGGTTTGGGAAAAAGAAGCCGCCTTTGGCCCAAGGCGGCGGCGGTGGAAGTGGCAGGGGAGGTGGTTCTGGGAACAATGGTGGGTGGGATAGTTGGGATGACGACGGTTTTAGATCGTCGACCGATTTGAGGAGGAATCAGTCGACCGGTAACTTTAGAGGCCGTGGTGTTCATGGTGGTAGTGGTGCTGGTGGATATGGAGGTGGGATGCCAGCCAAGTCGAGGTCCACGGAGGACATCACGAGATCACAATTGGAGGCTTCGGCGGCGAACAAGGAGAGCTTTTTCGCAAGGAAAATGGCAGAGAATGATTCGCGGCCTGACGGGCTACCGCCCTCACAGGGCGGGAAGTATGTCGGGTTTGGATCGAGCCCCGCCCCTTCGCAGAATCATAATGTGAATGCGCAAGGCGATGTGTTGTCTGTTGTATCTCAGGGTTTTGGAAGGTTATCTTTGGTTGCTGCATCGGCGGCTCAGTCGGCTGCAAGTGTAGTACAGGCCGGCACTAAAGATATCACTACTAAGGTGAAAGAAGGCGGCTATGATTACAAGGTGAATGAAACTGTTAATGTTGTCACTGCAAAGACTTCAGAGATTGGACAAAGGACTTGGGGGATTATGAAAGGGGTCATGGCATTAGCATCACAGAAGGTTGAAGAGTACACTAAAGATGGAACAAACGGGAAAGCTGAGAATAACTGGCAACGAAATGATAGTGAGCAGAATGGATACTATCAAGAGTTTAAACAGGAGAACAAAGAATGGAATTCTTCTTCCGTAGGGGGGCAATCATCGAGTGGGAATCTTAATTCTTACGGCTCTAGTTCTTGGGATGATTGGGACCAAAAAGACACAAGGAAGCAAGAGACTACTAAAGGAACGGGATCCAACAGCACTGATGGTTGGGCTGGCTGGGATGATGGCAAAGATGATGGATATGATAATGCTTACCAGAGTGCATCTATCTCAAAGGCTGGTCACAATGGGAAATCTGATTCAGCGAATCTCACGTGGTCTCAGCAAATACTGGTTGACTCATGTCGGACACAAATTAGAACTCAAACGGATGGATGCCAAGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

ArfGap

Protein Analysis

491

Amino Acids

52.51

Weight (kDa)

8.34

Isoelectric Point (pI)

45.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArfGap	PF01412	5 - 107	4.7e-35	Putative GTPase activating protein for Arf

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005633)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37550 AT2G37550
fragaria_vesca	FvH4_6g14160 FvH4_6g14160 FvH4_6g14160
malus_domestica	MD04G1124300.v1.1 MD12G1139600.v1.1
prunus_persica	Prupe.6G251500_v2.0.a1 Prupe.6G251500_v2.0.a1
pyrus_communis	pycom04g11210 pycom12g13410
rosa_chinensis	RchiOBHm_Chr3g0467101
rosa_laevigata	RLG00000007853 RLG00000024526
rosa_multiflora	Rmu_sc0000363.1_g000029 Rmu_sc0000723.1_g000006 Rmu_ssc0000065.1_g000001
rosa_roxburghii	Rroxscaffold_1G00027930 Rroxscaffold_6G00413890
rosa_rugosa	Rorug03G0085500 Rorug06G0179000
rosa_samantha	Rh2BG303700 Rh3AG133500 Rh3BG154700 Rh3CG154400 Rh3DG155500 Rh4CG176600
rosa_wichuraiana	Rw3G012620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	795
Acc36I	ACCTGC	1 cut(s)	36
AccI	GTMKAC	2 cut(s)	509, 533
AccII	CGCG	2 cut(s)	141, 706
AclWI	GGATC	6 cut(s)	165, 193, 333, 763, 1269, 1282
AcsI	RAATTY	1 cut(s)	1156
AcuI	CTGAAG	1 cut(s)	963
AcvI	CACGTG	1 cut(s)	1401
AcyI	GRCGYC	2 cut(s)	150, 352
AfaI	GTAC	4 cut(s)	296, 320, 885, 1052
AfiI	CCNNNNNNNGG	6 cut(s)	85, 346, 519, 612, 731, 1173
AgeI	ACCGGT	1 cut(s)	536
AgsI	TTSAA	2 cut(s)	335, 1046
AhdI	GACNNNNNGTC	1 cut(s)	190
AjnI	CCWGG	1 cut(s)	356
AjuI	GAANNNNNNNTTGG	2 cut(s)	398, 430
AluBI	AGCT	4 cut(s)	238, 258, 672, 1079
AluI	AGCT	4 cut(s)	238, 258, 672, 1079
Alw21I	GWGCWC	1 cut(s)	128
Alw26I	GTCTC	4 cut(s)	269, 377, 1250, 1410
AlwI	GGATC	6 cut(s)	165, 193, 333, 763, 1269, 1282
AoxI	GGCC	5 cut(s)	87, 417, 550, 707, 888
ApeKI	GCWGC	3 cut(s)	37, 851, 872
ApoI	RAATTY	1 cut(s)	1156
AsiGI	ACCGGT	1 cut(s)	536
AspLEI	GCGC	1 cut(s)	796
AspS9I	GGNCC	7 cut(s)	25, 87, 192, 364, 418, 615, 1231
AsuC2I	CCSGG	3 cut(s)	23, 42, 323
AsuHPI	GGTGA	3 cut(s)	280, 928, 955
AvaII	GGWCC	5 cut(s)	25, 192, 364, 615, 1231
AxyI	CCTNAGG	1 cut(s)	270
BamHI	GGATCC	1 cut(s)	1274
BanII	GRGCYC	2 cut(s)	147, 764
BauI	CACGAG	2 cut(s)	187, 631
BbrPI	CACGTG	1 cut(s)	1401
BbsI	GAAGAC	1 cut(s)	100
Bbv12I	GWGCWC	1 cut(s)	128
BbvI	GCAGC	3 cut(s)	49, 838, 859
BccI	CCATC	6 cut(s)	208, 1055, 1283, 1304, 1316, 1451
BceAI	ACGGC	3 cut(s)	277, 537, 1222
BciT130I	CCWGG	1 cut(s)	358
BciVI	GTATCC	2 cut(s)	106, 1112
BcnI	CCSGG	3 cut(s)	23, 42, 323
BcoDI	GTCTC	4 cut(s)	269, 377, 1250, 1410
BfaI	CTAG	3 cut(s)	251, 341, 1212
BfuAI	ACCTGC	1 cut(s)	36
BfuI	GTATCC	2 cut(s)	106, 1112
BglI	GCCNNNNNGGC	1 cut(s)	715
BglII	AGATCT	1 cut(s)	51
Bme1390I	CCNGG	4 cut(s)	23, 42, 323, 358
Bme18I	GGWCC	5 cut(s)	25, 192, 364, 615, 1231
BmeRI	GACNNNNNGTC	1 cut(s)	190
BmgT120I	GGNCC	7 cut(s)	25, 87, 192, 364, 418, 615, 1231
BmiI	GGNNCC	6 cut(s)	20, 26, 365, 366, 1232, 1276
BmrFI	CCNGG	4 cut(s)	23, 42, 323, 358
BmsI	GCATC	6 cut(s)	17, 588, 863, 1040, 1358, 1451
BoxI	GACNNNNGTC	1 cut(s)	1428
BpiI	GAAGAC	1 cut(s)	100
BpmI	CTGGAG	1 cut(s)	379
BpuMI	CCSGG	3 cut(s)	23, 42, 323
BsaAI	YACGTR	1 cut(s)	1401
BsaBI	GATNNNNATC	1 cut(s)	203
BsaHI	GRCGYC	2 cut(s)	150, 352
BsaI	GGTCTC	2 cut(s)	377, 1410
BsaJI	CCNNGG	9 cut(s)	41, 90, 139, 345, 356, 421, 553, 618, 1465
BsaWI	WCCGGW	1 cut(s)	536
Bsc4I	CCNNNNNNNGG	6 cut(s)	85, 346, 519, 612, 731, 1173
Bse118I	RCCGGY	2 cut(s)	536, 890
Bse1I	ACTGG	2 cut(s)	1094, 1422
Bse21I	CCTNAGG	1 cut(s)	270
Bse3DI	GCAATG	1 cut(s)	89
Bse8I	GATNNNNATC	1 cut(s)	203
BseBI	CCWGG	1 cut(s)	358
BseDI	CCNNGG	9 cut(s)	41, 90, 139, 345, 356, 421, 553, 618, 1465
BseGI	GGATG	6 cut(s)	493, 603, 1228, 1312, 1462, 1466
BseJI	GATNNNNATC	1 cut(s)	203
BseLI	CCNNNNNNNGG	6 cut(s)	85, 346, 519, 612, 731, 1173
BseMI	GCAATG	1 cut(s)	89
BseMII	CTCAG	4 cut(s)	836, 878, 1071, 1421
BseNI	ACTGG	2 cut(s)	1094, 1422
BseRI	GAGGAG	1 cut(s)	535
BseXI	GCAGC	3 cut(s)	49, 838, 859
BseYI	CCCAGC	1 cut(s)	1302
Bsh1236I	CGCG	2 cut(s)	141, 706
Bsh1285I	CGRYCG	2 cut(s)	513, 537
BshFI	GGCC	5 cut(s)	89, 419, 552, 709, 890
BshTI	ACCGGT	1 cut(s)	536
BsiEI	CGRYCG	2 cut(s)	513, 537
BsiHKAI	GWGCWC	1 cut(s)	128
BsiSI	CCGG	6 cut(s)	22, 41, 129, 322, 537, 891
BslFI	GGGAC	4 cut(s)	38, 339, 377, 1244
BslI	CCNNNNNNNGG	6 cut(s)	85, 346, 519, 612, 731, 1173
BsmAI	GTCTC	4 cut(s)	269, 377, 1250, 1410
BsmBI	CGTCTC	1 cut(s)	269
BsmFI	GGGAC	4 cut(s)	38, 339, 377, 1244
BsmI	GAATGC	1 cut(s)	796
BsnI	GGCC	5 cut(s)	89, 419, 552, 709, 890
Bso31I	GGTCTC	2 cut(s)	377, 1410
Bsp1286I	GDGCHC	3 cut(s)	128, 147, 764
Bsp143I	GATC	9 cut(s)	51, 170, 198, 204, 325, 503, 635, 755, 1274
BspANI	GGCC	5 cut(s)	89, 419, 552, 709, 890
BspCNI	CTCAG	4 cut(s)	835, 877, 1072, 1420
BspFNI	CGCG	2 cut(s)	141, 706
BspLI	GGNNCC	6 cut(s)	20, 26, 365, 366, 1232, 1276
BspMI	ACCTGC	1 cut(s)	36
BspPI	GGATC	6 cut(s)	165, 193, 333, 763, 1269, 1282
BspTNI	GGTCTC	2 cut(s)	377, 1410
BsrDI	GCAATG	1 cut(s)	89
BsrFI	RCCGGY	2 cut(s)	536, 890
BsrI	ACTGG	2 cut(s)	1094, 1422
BssAI	RCCGGY	2 cut(s)	536, 890
BssECI	CCNNGG	9 cut(s)	41, 90, 139, 345, 356, 421, 553, 618, 1465
BssMI	GATC	9 cut(s)	51, 170, 198, 204, 325, 503, 635, 755, 1274
BssNI	GRCGYC	2 cut(s)	150, 352
BssSI	CACGAG	2 cut(s)	187, 631
BssT1I	CCWWGG	2 cut(s)	421, 1465
Bst2BI	CACGAG	2 cut(s)	187, 631
Bst2UI	CCWGG	1 cut(s)	358
Bst4CI	ACNGT	3 cut(s)	104, 497, 955
Bst6I	CTCTTC	1 cut(s)	1041
BstACI	GRCGYC	2 cut(s)	150, 352
BstBAI	YACGTR	1 cut(s)	1401
BstC8I	GCNNGC	7 cut(s)	134, 143, 256, 313, 603, 892, 1300
BstDEI	CTNAG	7 cut(s)	118, 270, 822, 864, 912, 1080, 1407
BstDSI	CCRYGG	3 cut(s)	139, 553, 618
BstENI	CCTNNNNNAGG	1 cut(s)	344
BstF5I	GGATG	6 cut(s)	493, 603, 1228, 1312, 1462, 1466
BstFNI	CGCG	2 cut(s)	141, 706
BstHHI	GCGC	1 cut(s)	796
BstKTI	GATC	9 cut(s)	54, 173, 201, 207, 328, 506, 638, 758, 1277
BstMAI	GTCTC	4 cut(s)	269, 377, 1250, 1410
BstMBI	GATC	9 cut(s)	51, 170, 198, 204, 325, 503, 635, 755, 1274
BstMCI	CGRYCG	2 cut(s)	513, 537
BstNI	CCWGG	1 cut(s)	358
BstPAI	GACNNNNGTC	1 cut(s)	1428
BstSCI	CCNGG	4 cut(s)	21, 40, 321, 356
BstUI	CGCG	2 cut(s)	141, 706
BstV1I	GCAGC	3 cut(s)	49, 838, 859
BstV2I	GAAGAC	1 cut(s)	100
BstX2I	RGATCY	3 cut(s)	51, 198, 1274
BstYI	RGATCY	3 cut(s)	51, 198, 1274
Bsu36I	CCTNAGG	1 cut(s)	270
BsuI	GTATCC	2 cut(s)	106, 1112
BsuRI	GGCC	5 cut(s)	89, 419, 552, 709, 890
BtgI	CCRYGG	3 cut(s)	139, 553, 618
BtgZI	GCGATG	1 cut(s)	816
BtsCI	GGATG	6 cut(s)	493, 603, 1228, 1312, 1462, 1466
BtsI	GCAGTG	1 cut(s)	966
BtsIMutI	CAGTG	2 cut(s)	966, 1284
BveI	ACCTGC	1 cut(s)	36
Cac8I	GCNNGC	7 cut(s)	134, 143, 256, 313, 603, 892, 1300
CfoI	GCGC	1 cut(s)	796
Cfr10I	RCCGGY	2 cut(s)	536, 890
Cfr13I	GGNCC	7 cut(s)	25, 87, 192, 364, 418, 615, 1231
Cfr42I	CCGCGG	1 cut(s)	142
CseI	GACGC	2 cut(s)	139, 341
Csp6I	GTAC	4 cut(s)	295, 319, 884, 1051
CspAI	ACCGGT	1 cut(s)	536
CviAII	CATG	4 cut(s)	112, 563, 1021, 1428
CviQI	GTAC	4 cut(s)	295, 319, 884, 1051
DdeI	CTNAG	7 cut(s)	118, 270, 822, 864, 912, 1080, 1407
DpnI	GATC	9 cut(s)	53, 172, 200, 206, 327, 505, 637, 757, 1276
DpnII	GATC	9 cut(s)	51, 170, 198, 204, 325, 503, 635, 755, 1274
DraI	TTTAAA	1 cut(s)	1135
DriI	GACNNNNNGTC	1 cut(s)	190
Eam1104I	CTCTTC	1 cut(s)	1041
Eam1105I	GACNNNNNGTC	1 cut(s)	190
EarI	CTCTTC	1 cut(s)	1041
Eco130I	CCWWGG	2 cut(s)	421, 1465
Eco24I	GRGCYC	2 cut(s)	147, 764
Eco31I	GGTCTC	2 cut(s)	377, 1410
Eco32I	GATATC	2 cut(s)	283, 904
Eco47I	GGWCC	5 cut(s)	25, 192, 364, 615, 1231
Eco57I	CTGAAG	1 cut(s)	963
Eco72I	CACGTG	1 cut(s)	1401
Eco81I	CCTNAGG	1 cut(s)	270
EcoNI	CCTNNNNNAGG	1 cut(s)	344
EcoO109I	RGGNCCY	2 cut(s)	25, 364
EcoRI	GAATTC	1 cut(s)	1156
EcoRII	CCWGG	1 cut(s)	356
EcoRV	GATATC	2 cut(s)	283, 904
EcoT14I	CCWWGG	2 cut(s)	421, 1465
EcoT38I	GRGCYC	2 cut(s)	147, 764
ErhI	CCWWGG	2 cut(s)	421, 1465
Esp3I	CGTCTC	1 cut(s)	269
FaeI	CATG	4 cut(s)	115, 566, 1024, 1431
FalI	AAGNNNNNCTT	4 cut(s)	656, 688, 826, 858
FaqI	GGGAC	4 cut(s)	38, 339, 377, 1244
FatI	CATG	4 cut(s)	111, 562, 1020, 1427
FauI	CCCGC	4 cut(s)	87, 134, 728, 772
FblI	GTMKAC	2 cut(s)	509, 533
FokI	GGATG	5 cut(s)	500, 610, 1235, 1319, 1469
FriOI	GRGCYC	2 cut(s)	147, 764
FspBI	CTAG	3 cut(s)	251, 341, 1212
FspI	TGCGCA	1 cut(s)	795
GlaI	GCGC	1 cut(s)	795
GsaI	CCCAGC	1 cut(s)	1306
GsuI	CTGGAG	1 cut(s)	379
HaeIII	GGCC	5 cut(s)	89, 419, 552, 709, 890
HapII	CCGG	6 cut(s)	22, 41, 129, 322, 537, 891
HgaI	GACGC	2 cut(s)	139, 341
HhaI	GCGC	1 cut(s)	796
Hin1I	GRCGYC	2 cut(s)	150, 352
Hin1II	CATG	4 cut(s)	115, 566, 1024, 1431
Hin6I	GCGC	1 cut(s)	794
HinP1I	GCGC	1 cut(s)	794
HincII	GTYRAC	3 cut(s)	510, 534, 1423
HindII	GTYRAC	3 cut(s)	510, 534, 1423
HinfI	GANTC	9 cut(s)	185, 331, 526, 701, 778, 1192, 1385, 1393, 1424
HpaII	CCGG	6 cut(s)	22, 41, 129, 322, 537, 891
HphI	GGTGA	3 cut(s)	280, 928, 955
Hpy166II	GTNNAC	8 cut(s)	61, 154, 319, 510, 534, 618, 1053, 1423
Hpy188I	TCNGA	6 cut(s)	170, 196, 330, 982, 1384, 1434
Hpy188III	TCNNGA	5 cut(s)	202, 208, 380, 631, 1127
Hpy8I	GTNNAC	8 cut(s)	61, 154, 319, 510, 534, 618, 1053, 1423
Hpy99I	CGWCG	3 cut(s)	18, 497, 511
HpyAV	CCTTC	5 cut(s)	256, 780, 828, 917, 1033
HpyCH4III	ACNGT	3 cut(s)	104, 497, 955
HpyCH4IV	ACGT	1 cut(s)	1400
HpyCH4V	TGCA	5 cut(s)	31, 854, 875, 971, 1349
HpyF3I	CTNAG	7 cut(s)	118, 270, 822, 864, 912, 1080, 1407
HpySE526I	ACGT	1 cut(s)	1400
Hsp92I	GRCGYC	2 cut(s)	150, 352
Hsp92II	CATG	4 cut(s)	115, 566, 1024, 1431
HspAI	GCGC	1 cut(s)	794
KflI	GGGWCCC	1 cut(s)	364
KroI	GCCGGC	1 cut(s)	890
KroNI	GCCGGC	1 cut(s)	892
KspI	CCGCGG	1 cut(s)	142
Kzo9I	GATC	9 cut(s)	51, 170, 198, 204, 325, 503, 635, 755, 1274
LmnI	GCTCC	3 cut(s)	24, 71, 131
Lsp1109I	GCAGC	3 cut(s)	49, 838, 859
LweI	GCATC	6 cut(s)	17, 588, 863, 1040, 1358, 1451
MaeI	CTAG	3 cut(s)	251, 341, 1212
MaeII	ACGT	1 cut(s)	1400
MaeIII	GTNAC	5 cut(s)	175, 286, 539, 964, 1367
MalI	GATC	9 cut(s)	53, 172, 200, 206, 327, 505, 637, 757, 1276
MboI	GATC	9 cut(s)	51, 170, 198, 204, 325, 503, 635, 755, 1274
MboII	GAAGA	6 cut(s)	85, 100, 223, 1058, 1152, 1155
MfeI	CAATTG	1 cut(s)	641
MflI	RGATCY	3 cut(s)	51, 198, 1274
MhlI	GDGCHC	3 cut(s)	128, 147, 764
MluCI	AATT	4 cut(s)	641, 1156, 1198, 1440
MlyI	GAGTC	2 cut(s)	179, 1418
MmeI	TCCRAC	2 cut(s)	1302, 1412
MroNI	GCCGGC	1 cut(s)	890
MseI	TTAA	3 cut(s)	957, 1134, 1197
MspA1I	CMGCKG	1 cut(s)	141
MspI	CCGG	6 cut(s)	22, 41, 129, 322, 537, 891
MspR9I	CCNGG	4 cut(s)	23, 42, 323, 358
MssI	GTTTAAAC	1 cut(s)	1135
MunI	CAATTG	1 cut(s)	641
Mva1269I	GAATGC	1 cut(s)	796
MvaI	CCWGG	1 cut(s)	358
MvnI	CGCG	2 cut(s)	141, 706
NaeI	GCCGGC	1 cut(s)	892
NciI	CCSGG	3 cut(s)	23, 42, 323
NdeII	GATC	9 cut(s)	51, 170, 198, 204, 325, 503, 635, 755, 1274
NgoMIV	GCCGGC	1 cut(s)	890
NlaIII	CATG	4 cut(s)	115, 566, 1024, 1431
NlaIV	GGNNCC	6 cut(s)	20, 26, 365, 366, 1232, 1276
NmeAIII	GCCGAG	2 cut(s)	126, 370
NmuCI	GTSAC	4 cut(s)	175, 286, 964, 1367
NsbI	TGCGCA	1 cut(s)	795
PcsI	WCGNNNNNNNCGW	1 cut(s)	171
PctI	GAATGC	1 cut(s)	796
PdiI	GCCGGC	1 cut(s)	892
PfeI	GAWTC	7 cut(s)	331, 526, 701, 778, 1192, 1385, 1393
PfoI	TCCNGGA	1 cut(s)	21
PinAI	ACCGGT	1 cut(s)	536
PleI	GAGTC	2 cut(s)	179, 1418
PmaCI	CACGTG	1 cut(s)	1401
PmeI	GTTTAAAC	1 cut(s)	1135
PmlI	CACGTG	1 cut(s)	1401
PpsI	GAGTC	2 cut(s)	179, 1418
Ppu21I	YACGTR	1 cut(s)	1401
PpuMI	RGGWCCY	2 cut(s)	25, 364
PshAI	GACNNNNGTC	1 cut(s)	1428
Psp5II	RGGWCCY	2 cut(s)	25, 364
Psp6I	CCWGG	1 cut(s)	356
PspCI	CACGTG	1 cut(s)	1401
PspFI	CCCAGC	1 cut(s)	1302
PspGI	CCWGG	1 cut(s)	356
PspN4I	GGNNCC	6 cut(s)	20, 26, 365, 366, 1232, 1276
PspPI	GGNCC	7 cut(s)	25, 87, 192, 364, 418, 615, 1231
PspPPI	RGGWCCY	2 cut(s)	25, 364
PsuI	RGATCY	3 cut(s)	51, 198, 1274
RsaI	GTAC	4 cut(s)	296, 320, 885, 1052
RsaNI	GTAC	4 cut(s)	295, 319, 884, 1051
SacII	CCGCGG	1 cut(s)	142
SalI	GTCGAC	2 cut(s)	508, 532
SaqAI	TTAA	3 cut(s)	957, 1134, 1197
Sau3AI	GATC	9 cut(s)	51, 170, 198, 204, 325, 503, 635, 755, 1274
Sau96I	GGNCC	7 cut(s)	25, 87, 192, 364, 418, 615, 1231
SchI	GAGTC	2 cut(s)	179, 1418
ScrFI	CCNGG	4 cut(s)	23, 42, 323, 358
SduI	GDGCHC	3 cut(s)	128, 147, 764
SfaNI	GCATC	6 cut(s)	17, 588, 863, 1040, 1358, 1451
Sfr303I	CCGCGG	1 cut(s)	142
SgrBI	CCGCGG	1 cut(s)	142
SinI	GGWCC	5 cut(s)	25, 192, 364, 615, 1231
Sse9I	AATT	4 cut(s)	641, 1156, 1198, 1440
SspMI	CTAG	3 cut(s)	251, 341, 1212
StyD4I	CCNGG	4 cut(s)	21, 40, 321, 356
StyI	CCWWGG	2 cut(s)	421, 1465
TaaI	ACNGT	3 cut(s)	104, 497, 955
TaiI	ACGT	1 cut(s)	1403
TaqI	TCGA	5 cut(s)	509, 533, 611, 758, 1184
TaqII	GACCGA	1 cut(s)	527
TasI	AATT	4 cut(s)	641, 1156, 1198, 1440
TatI	WGTACW	3 cut(s)	294, 883, 1050
TauI	GCSGC	8 cut(s)	8, 412, 429, 432, 659, 709, 863, 930
TfiI	GAWTC	7 cut(s)	331, 526, 701, 778, 1192, 1385, 1393
Tru1I	TTAA	3 cut(s)	957, 1134, 1197
Tru9I	TTAA	3 cut(s)	957, 1134, 1197
TscAI	CASTG	2 cut(s)	973, 1291
TseFI	GTSAC	4 cut(s)	175, 286, 964, 1367
TseI	GCWGC	3 cut(s)	37, 851, 872
Tsp45I	GTSAC	4 cut(s)	175, 286, 964, 1367
TspDTI	ATGAA	4 cut(s)	100, 551, 963, 1025
TspGWI	ACGGA	5 cut(s)	163, 293, 635, 1156, 1469
TspRI	CASTG	2 cut(s)	973, 1291
VpaK11BI	GGWCC	5 cut(s)	25, 192, 364, 615, 1231
XagI	CCTNNNNNAGG	1 cut(s)	344
XapI	RAATTY	1 cut(s)	1156
XmiI	GTMKAC	2 cut(s)	509, 533
XspI	CTAG	3 cut(s)	251, 341, 1212

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.