RLG00000007853

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005633
Physical Location & Seq
Reverse (-)
22001405 .. 22018405
17001 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007853

Sequence Viewer

Length: 903 bp
ATGTCGCAAATTTTGAAGAATCCGCAATGGGCTTTGGTCTCCTACGTCCTCTTCATGTGCCTAGAGTGCTTCGGCAAGCACCGCGGCCTCAGCGTCCACGTCTCCTTCGTCAGATCTGTGACCATGGACTCCTGGTCCGAGATCAAGATCAAGAAGATGAAGGCCGGCGGCAACGAGAGGCTCAACGCCTTCCTCGCCAGCTACGGCGTCTCTAAGGAGACCGATATTGTCACCAAGTATAACACCAACGCTACCAGCAGCCCGTCCCCTGGGTTTTCACAGCGGAGGCTCCATGGAGAGCGACGGAAACTTGCGGATAAAGTTGCTGCAGCTGGATTTTTTGTGGTGGTTCCTGATTTCTTTTATGGCGACCCTATGAATCTTGATAATCCTCAATTCGACCGTGAGGCATGGCATAAAGCCCATAGCGTGGTTAGTGTATATAACTGTACATATACAATCTTTGGATATGAAAACCCAAATTTAAGGAAACTTGCGGATACAGTTGCTGCAGCTGGATTTTTCGTGGTGGTTCCCGATTTCTTTTATGGCGACCCTGTAAATCTCGATAATCCTCAATTCGACCGAGAGTCCTGGATTAAAGCTCACAGCAAGAAAATCACCACTATGTTGACTGTGCCCGCTCTATTGAAGCTCGCAGACAAAGTTGCAGCTGCTGGCTTCTTTGTTGTGGTCCCTGACTTCTTTTATGGAGACCCTTATGTACCTGCAGCTGATGGATCCTTTGACTCTCTACCTACTTGGCTGAAAGCCAAGGTTGTAGTTGAACTTGCAAAGGGTGACTTTATCCAAGCTGCTGTTCTGGCTCATCCTTCATTTGTCACTGTGGATGATATCAAGGGTAAGAGTTATCTATTCAATTATCAAGAAATATCTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap

Protein Analysis

301

Amino Acids

33.46

Weight (kDa)

8.27

Isoelectric Point (pI)

28.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 6 - 75 6.7e-21 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 736
AccB7I CCANNNNNTGG 1 cut(s) 430
AccBSI CCGCTC 1 cut(s) 644
AccII CGCG 1 cut(s) 84
AciI CCGC 8 cut(s) 23, 82, 84, 168, 283, 314, 497, 642
AclWI GGATC 2 cut(s) 735, 748
AcsI RAATTY 2 cut(s) 9, 481
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 2 cut(s) 451, 726
AfiI CCNNNNNNNGG 2 cut(s) 269, 430
AgsI TTSAA 4 cut(s) 16, 652, 788, 880
AhdI GACNNNNNGTC 2 cut(s) 133, 589
AjiI CACGTC 1 cut(s) 100
AjnI CCWGG 3 cut(s) 131, 268, 593
AjuI GAANNNNNNNTTGG 2 cut(s) 804, 836
AluBI AGCT 8 cut(s) 201, 332, 515, 605, 655, 674, 734, 815
AluI AGCT 8 cut(s) 201, 332, 515, 605, 655, 674, 734, 815
Alw26I GTCTC 5 cut(s) 43, 106, 212, 214, 708
AlwI GGATC 2 cut(s) 735, 748
AlwNI CAGNNNCTG 2 cut(s) 509, 677
AoxI GGCC 2 cut(s) 85, 162
ApeKI GCWGC 9 cut(s) 258, 326, 329, 509, 512, 671, 674, 731, 815
ApoI RAATTY 2 cut(s) 9, 481
AspS9I GGNCC 2 cut(s) 135, 694
AsuHPI GGTGA 3 cut(s) 223, 613, 812
AvaII GGWCC 2 cut(s) 135, 694
BaeGI GKGCMC 1 cut(s) 642
BamHI GGATCC 1 cut(s) 740
BbvCI CCTCAGC 1 cut(s) 89
BbvI GCAGC 9 cut(s) 270, 313, 341, 496, 524, 661, 683, 743, 802
BccI CCATC 1 cut(s) 731
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 3 cut(s) 133, 270, 595
BciVI GTATCC 1 cut(s) 493
BcoDI GTCTC 5 cut(s) 43, 106, 212, 214, 708
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 3 cut(s) 327, 510, 729
BfuAI ACCTGC 1 cut(s) 736
BfuI GTATCC 1 cut(s) 493
BglII AGATCT 1 cut(s) 113
Bme1390I CCNGG 3 cut(s) 133, 270, 595
Bme18I GGWCC 2 cut(s) 135, 694
BmeRI GACNNNNNGTC 2 cut(s) 133, 589
BmgBI CACGTC 1 cut(s) 100
BmgT120I GGNCC 2 cut(s) 135, 694
BmiI GGNNCC 5 cut(s) 290, 351, 534, 696, 742
BmrFI CCNGG 3 cut(s) 133, 270, 595
Bpu10I CCTNAGC 1 cut(s) 89
BsaBI GATNNNNATC 1 cut(s) 146
BsaHI GRCGYC 1 cut(s) 207
BsaI GGTCTC 3 cut(s) 43, 212, 708
BsaJI CCNNGG 6 cut(s) 82, 123, 268, 269, 292, 774
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 430
Bse118I RCCGGY 1 cut(s) 164
Bse3DI GCAATG 1 cut(s) 32
Bse8I GATNNNNATC 1 cut(s) 146
BseBI CCWGG 3 cut(s) 133, 270, 595
BseDI CCNNGG 6 cut(s) 82, 123, 268, 269, 292, 774
BseGI GGATG 2 cut(s) 829, 856
BseJI GATNNNNATC 1 cut(s) 146
BseLI CCNNNNNNNGG 2 cut(s) 269, 430
BseMI GCAATG 1 cut(s) 32
BseMII CTCAG 1 cut(s) 103
BseSI GKGCMC 1 cut(s) 642
BseXI GCAGC 9 cut(s) 270, 313, 341, 496, 524, 661, 683, 743, 802
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 2 cut(s) 403, 586
BshFI GGCC 2 cut(s) 87, 164
BsiEI CGRYCG 2 cut(s) 403, 586
BsiSI CCGG 1 cut(s) 165
BslFI GGGAC 2 cut(s) 250, 680
BslI CCNNNNNNNGG 2 cut(s) 269, 430
BsmAI GTCTC 5 cut(s) 43, 106, 212, 214, 708
BsmBI CGTCTC 2 cut(s) 106, 214
BsmFI GGGAC 2 cut(s) 250, 680
BsnI GGCC 2 cut(s) 87, 164
Bso31I GGTCTC 3 cut(s) 43, 212, 708
Bsp1286I GDGCHC 1 cut(s) 642
Bsp1407I TGTACA 1 cut(s) 449
Bsp143I GATC 4 cut(s) 113, 141, 147, 740
Bsp19I CCATGG 2 cut(s) 123, 292
BspACI CCGC 8 cut(s) 23, 82, 84, 168, 283, 314, 497, 642
BspANI GGCC 2 cut(s) 87, 164
BspCNI CTCAG 1 cut(s) 102
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 5 cut(s) 290, 351, 534, 696, 742
BspMAI CTGCAG 3 cut(s) 331, 514, 733
BspMI ACCTGC 1 cut(s) 736
BspPI GGATC 2 cut(s) 735, 748
BspTNI GGTCTC 3 cut(s) 43, 212, 708
BsrBI CCGCTC 1 cut(s) 644
BsrDI GCAATG 1 cut(s) 32
BsrFI RCCGGY 1 cut(s) 164
BsrGI TGTACA 1 cut(s) 449
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 6 cut(s) 82, 123, 268, 269, 292, 774
BssMI GATC 4 cut(s) 113, 141, 147, 740
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 3 cut(s) 123, 292, 774
Bst2UI CCWGG 3 cut(s) 133, 270, 595
Bst4CI ACNGT 5 cut(s) 404, 449, 505, 637, 847
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 207
BstAUI TGTACA 1 cut(s) 449
BstC8I GCNNGC 6 cut(s) 77, 166, 199, 642, 657, 679
BstDEI CTNAG 2 cut(s) 89, 213
BstDSI CCRYGG 3 cut(s) 82, 123, 292
BstF5I GGATG 2 cut(s) 829, 856
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 4 cut(s) 116, 144, 150, 743
BstMAI GTCTC 5 cut(s) 43, 106, 212, 214, 708
BstMBI GATC 4 cut(s) 113, 141, 147, 740
BstMCI CGRYCG 2 cut(s) 403, 586
BstMWI GCNNNNNNNGC 5 cut(s) 66, 81, 90, 194, 824
BstNI CCWGG 3 cut(s) 133, 270, 595
BstSCI CCNGG 3 cut(s) 131, 268, 593
BstSFI CTRYAG 3 cut(s) 327, 510, 729
BstSLI GKGCMC 1 cut(s) 642
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 9 cut(s) 270, 313, 341, 496, 524, 661, 683, 743, 802
BstX2I RGATCY 2 cut(s) 113, 740
BstYI RGATCY 2 cut(s) 113, 740
BsuI GTATCC 1 cut(s) 493
BsuRI GGCC 2 cut(s) 87, 164
BtgI CCRYGG 3 cut(s) 82, 123, 292
BtrI CACGTC 1 cut(s) 100
BtsCI GGATG 2 cut(s) 829, 856
BtsIMutI CAGTG 1 cut(s) 843
BveI ACCTGC 1 cut(s) 736
Cac8I GCNNGC 6 cut(s) 77, 166, 199, 642, 657, 679
CaiI CAGNNNCTG 2 cut(s) 509, 677
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 2 cut(s) 135, 694
Cfr42I CCGCGG 1 cut(s) 85
CseI GACGC 2 cut(s) 82, 196
Csp6I GTAC 2 cut(s) 450, 725
CviAII CATG 4 cut(s) 55, 124, 293, 411
CviQI GTAC 2 cut(s) 450, 725
DdeI CTNAG 2 cut(s) 89, 213
DpnI GATC 4 cut(s) 115, 143, 149, 742
DpnII GATC 4 cut(s) 113, 141, 147, 740
DriI GACNNNNNGTC 2 cut(s) 133, 589
Eam1104I CTCTTC 1 cut(s) 56
Eam1105I GACNNNNNGTC 2 cut(s) 133, 589
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 3 cut(s) 123, 292, 774
Eco31I GGTCTC 3 cut(s) 43, 212, 708
Eco32I GATATC 1 cut(s) 856
Eco47I GGWCC 2 cut(s) 135, 694
EcoRII CCWGG 3 cut(s) 131, 268, 593
EcoRV GATATC 1 cut(s) 856
EcoT14I CCWWGG 3 cut(s) 123, 292, 774
ErhI CCWWGG 3 cut(s) 123, 292, 774
Esp3I CGTCTC 2 cut(s) 106, 214
FaeI CATG 4 cut(s) 58, 127, 296, 414
FalI AAGNNNNNCTT 2 cut(s) 788, 820
FaqI GGGAC 2 cut(s) 250, 680
FatI CATG 4 cut(s) 54, 123, 292, 410
FauI CCCGC 1 cut(s) 649
FokI GGATG 2 cut(s) 816, 863
FspBI CTAG 1 cut(s) 62
HaeIII GGCC 2 cut(s) 87, 164
HapII CCGG 1 cut(s) 165
HgaI GACGC 2 cut(s) 82, 196
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 4 cut(s) 58, 127, 296, 414
HincII GTYRAC 1 cut(s) 633
HindII GTYRAC 1 cut(s) 633
HinfI GANTC 5 cut(s) 19, 128, 379, 590, 749
HpaII CCGG 1 cut(s) 165
HphI GGTGA 3 cut(s) 223, 613, 812
Hpy166II GTNNAC 2 cut(s) 97, 633
Hpy188I TCNGA 2 cut(s) 113, 139
Hpy188III TCNNGA 7 cut(s) 145, 151, 353, 383, 536, 566, 887
Hpy8I GTNNAC 2 cut(s) 97, 633
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 4 cut(s) 115, 154, 199, 843
HpyCH4III ACNGT 5 cut(s) 404, 449, 505, 637, 847
HpyCH4IV ACGT 2 cut(s) 45, 99
HpyCH4V TGCA 5 cut(s) 329, 512, 671, 731, 794
HpyF10VI GCNNNNNNNGC 5 cut(s) 66, 81, 90, 194, 824
HpyF3I CTNAG 2 cut(s) 89, 213
HpySE526I ACGT 2 cut(s) 45, 99
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 4 cut(s) 58, 127, 296, 414
KroI GCCGGC 1 cut(s) 164
KroNI GCCGGC 1 cut(s) 166
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 4 cut(s) 113, 141, 147, 740
LmnI GCTCC 1 cut(s) 294
Lsp1109I GCAGC 9 cut(s) 270, 313, 341, 496, 524, 661, 683, 743, 802
MaeI CTAG 1 cut(s) 62
MaeII ACGT 2 cut(s) 45, 99
MaeIII GTNAC 4 cut(s) 118, 229, 800, 841
MalI GATC 4 cut(s) 115, 143, 149, 742
MbiI CCGCTC 1 cut(s) 644
MboI GATC 4 cut(s) 113, 141, 147, 740
MboII GAAGA 3 cut(s) 28, 43, 166
MflI RGATCY 2 cut(s) 113, 740
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 5 cut(s) 9, 395, 481, 578, 880
MlyI GAGTC 3 cut(s) 122, 599, 743
MnlI CCTC 8 cut(s) 59, 98, 171, 203, 279, 400, 402, 585
MroNI GCCGGC 1 cut(s) 164
MseI TTAA 2 cut(s) 485, 600
MslI CAYNNNNRTG 1 cut(s) 626
MspA1I CMGCKG 6 cut(s) 84, 283, 332, 515, 674, 734
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 3 cut(s) 133, 270, 595
MvaI CCWGG 3 cut(s) 133, 270, 595
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 5 cut(s) 66, 81, 90, 194, 824
NaeI GCCGGC 1 cut(s) 166
NcoI CCATGG 2 cut(s) 123, 292
NdeII GATC 4 cut(s) 113, 141, 147, 740
NgoMIV GCCGGC 1 cut(s) 164
NlaIII CATG 4 cut(s) 58, 127, 296, 414
NlaIV GGNNCC 5 cut(s) 290, 351, 534, 696, 742
NmuCI GTSAC 4 cut(s) 118, 229, 800, 841
PasI CCCWGGG 1 cut(s) 269
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PdiI GCCGGC 1 cut(s) 166
PfeI GAWTC 2 cut(s) 19, 379
PflMI CCANNNNNTGG 1 cut(s) 430
PfoI TCCNGGA 1 cut(s) 593
PleI GAGTC 3 cut(s) 122, 598, 743
PpsI GAGTC 3 cut(s) 122, 598, 743
Psp6I CCWGG 3 cut(s) 131, 268, 593
PspGI CCWGG 3 cut(s) 131, 268, 593
PspN4I GGNNCC 5 cut(s) 290, 351, 534, 696, 742
PspPI GGNCC 2 cut(s) 135, 694
PstI CTGCAG 3 cut(s) 331, 514, 733
PstNI CAGNNNCTG 2 cut(s) 509, 677
PsuI RGATCY 2 cut(s) 113, 740
PvuII CAGCTG 4 cut(s) 332, 515, 674, 734
RsaI GTAC 2 cut(s) 451, 726
RsaNI GTAC 2 cut(s) 450, 725
RseI CAYNNNNRTG 1 cut(s) 626
SacII CCGCGG 1 cut(s) 85
SaqAI TTAA 2 cut(s) 485, 600
Sau3AI GATC 4 cut(s) 113, 141, 147, 740
Sau96I GGNCC 2 cut(s) 135, 694
SchI GAGTC 3 cut(s) 122, 599, 743
ScrFI CCNGG 3 cut(s) 133, 270, 595
SduI GDGCHC 1 cut(s) 642
SfcI CTRYAG 3 cut(s) 327, 510, 729
Sfr303I CCGCGG 1 cut(s) 85
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 2 cut(s) 135, 694
SmiMI CAYNNNNRTG 1 cut(s) 626
Sse9I AATT 5 cut(s) 9, 395, 481, 578, 880
SsiI CCGC 8 cut(s) 23, 82, 84, 168, 283, 314, 497, 642
SspMI CTAG 1 cut(s) 62
StyD4I CCNGG 3 cut(s) 131, 268, 593
StyI CCWWGG 3 cut(s) 123, 292, 774
TaaI ACNGT 5 cut(s) 404, 449, 505, 637, 847
TaiI ACGT 2 cut(s) 48, 102
TaqI TCGA 3 cut(s) 399, 567, 582
TaqII GACCGA 2 cut(s) 236, 600
TasI AATT 5 cut(s) 9, 395, 481, 578, 880
TatI WGTACW 1 cut(s) 449
TauI GCSGC 2 cut(s) 87, 171
TfiI GAWTC 2 cut(s) 19, 379
Tru1I TTAA 2 cut(s) 485, 600
Tru9I TTAA 2 cut(s) 485, 600
TscAI CASTG 1 cut(s) 850
TseFI GTSAC 4 cut(s) 118, 229, 800, 841
TseI GCWGC 9 cut(s) 258, 326, 329, 509, 512, 671, 674, 731, 815
Tsp45I GTSAC 4 cut(s) 118, 229, 800, 841
TspDTI ATGAA 5 cut(s) 43, 173, 392, 486, 825
TspGWI ACGGA 1 cut(s) 319
TspRI CASTG 1 cut(s) 850
Van91I CCANNNNNTGG 1 cut(s) 430
VpaK11BI GGWCC 2 cut(s) 135, 694
XapI RAATTY 2 cut(s) 9, 481
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.