Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004979

Physical Location & Seq

Genomic Coordinates Forward (+)

21339498 .. 21342272

Gene Structure 2775 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1127500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1515 bp

ATGGGTAGGATTACTGTGGCGACGATTGTGAGCCTGTGGGTAATTCCCATCTCCATCCTCGTCAATCACATTGTTCCCGAGCCCTACATGGATGAGATATTCCATGTGCCTCAAGCTCAACAGTACTGTAATGGCAATTTCAGAAGCTGGGATCCCATGATCACCACTCCTCCTGGCCTGTACTATCTTTCACTAGCTCATGTTGCGTATTTGTTTCCAGGCATGTCATTGGTAAAAGCAGACCATTCGTTTTCTGAAGGCTGCTCCACAGCGATTCTTCGCTCTGTCAATGGTGTTTTGGCTGTATTTTGCAGCGTTCTTGTGTATGAGATAATCATTCACTTGAGACCGGCTCTTGATGAAAGAAGAGCAACACTTTACGCGGTGGTGCTAGCCATGTACCCTCTTCATTGGTTCTTCACTTTTCTCTATTATACAGATGTTGTGTCGCTTGCGGCAGTGCTCGCTATGCATCTAGCGTGTTTGAAGAAGAAGTACTGGTTTAGTGCATTGCTTGGTGCTGTGGCAGTTGTTATTCGGCAAACAAATATTATATGGATGCTTTTTGTTTGTTGCAATGAGGTTATAAATATTACAATGGCTGACCATAAAGATAAGATAGACACAATTAGGATAAAAGGTCAGCCAACTCCTTCAAACACTGTTACTTTAGGCCCAAACTTGAGAAAGCGGAAGGCCAGCACTTCAGTGGATGCTGGAACTCATTTAATCTCGACTAGAAAAATTTCTCTTCAAACTTGCAAATTAGGTTTTCTTGATGAAATTAAGGCCATCTTTCCAAAATTATGGCACATGAAGTTGGAACTTTTGTTTTCTTTTAGCCCTTTTATTCTAGTATTAGCAGCCTTTGTTGCCCTTGTTCGGTGGAATGGGAGTGTGGTTCTAGGTGCAAAAGATGCTCATGCCGTTTCTCCTCATTTTGCACAGATCTTGTATTTTGGTCTATTTTCTGCCCTTGCTATGGCTCCTACACTCTTTAGTTTGAGACAAACGGTACACTTATTCTGGTCAATGTGGAAGAATAAGTTCCTTACCTTCTTTCAGATGTCTTTGGCACTCATTGCTGGCTTCATCGCTGTCCATTTTTTCAGCATAGCTCATCCCTATCTTCTGGCTGACAATCGGCATTATCCCTTTTATCTTTGGCGGAAGGTCATCAAAGCTCATTGGTCAATGAAGTACCTCCTCATCCCACTTTATGTTTATTCATGGCTTTCCATCATCACTAGATTGGGGAAATTTCAGAGGAAGATTTGGGTGCTGGCATTTGTCTTGGCTACTGCTGCAGTTTTGGTTCCCGCACCGCTGATTGAGTTTAGATATTGTACCATTCCATTCTTTTTCTTGATCCTACATTCCCACGCTGACGATTATCGAAGTTGGCTCGTCACGGGGCTACTGTACATAACTGTCAACATATACACAATGATGATGTTCTTGTATCGACCATTCCATTGGTCCCATGAGCCAGGGACCCAGCGGTTTATATGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006486 GO:0006487 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0016020 GO:0016740 GO:0016757 GO:0016758 GO:0019538 GO:0031984 GO:0032501 GO:0032502 GO:0034645 GO:0036211 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046527 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0070085 GO:0071704 GO:0098827 GO:0099402 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko01100 map00510 map01100

Pfam Domains

Protein Families

DIE2_ALG10

Protein Analysis

505

Amino Acids

57.93

Weight (kDa)

9.55

Isoelectric Point (pI)

40.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIE2_ALG10	PF04922	25 - 457	5.7e-124	DIE2/ALG10 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004979)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02410
fragaria_vesca	FvH4_6g13910 FvH4_6g13910 FvH4_6g13910 FvH4_6g13910 FvH4_6g13910 FvH4_6g13910 FvH4_6g13910 FvH4_6g13910 FvH4_6g13910 FvH4_6g13910
malus_domestica	MD04G1127500.v1.1 MD12G1140900.v1.1
prunus_persica	Prupe.6G254200_v2.0.a1 Prupe.6G254200_v2.0.a1
pyrus_communis	pycom04g11440 pycom126g00050 pycom12g13580
rosa_chinensis	RchiOBHm_Chr3g0466751
rosa_laevigata	RLG00000024556
rosa_multiflora	Rmu_sc0026659.1_g000002 Rmu_sc0035686.1_g000001
rosa_roxburghii	Rroxscaffold_6G00414200
rosa_rugosa	Rorug03G0083600 Rorug03G0083700
rosa_samantha	Rh3AG130700 Rh3BG151700 Rh3CG151600 Rh3DG152400
rosa_wichuraiana	Rw3G012330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	587
AccII	CGCG	1 cut(s)	383
AciI	CCGC	7 cut(s)	383, 455, 691, 1168, 1320, 1325, 1501
AclWI	GGATC	3 cut(s)	146, 159, 1363
AcsI	RAATTY	2 cut(s)	744, 1259
AcuI	CTGAAG	2 cut(s)	276, 690
AfaI	GTAC	8 cut(s)	125, 182, 401, 497, 1017, 1202, 1348, 1424
AfiI	CCNNNNNNNGG	2 cut(s)	882, 982
AgsI	TTSAA	3 cut(s)	487, 657, 755
AjnI	CCWGG	3 cut(s)	172, 217, 1489
AleI	CACNNNNGTG	1 cut(s)	707
AluBI	AGCT	5 cut(s)	116, 147, 197, 1118, 1184
AluI	AGCT	5 cut(s)	116, 147, 197, 1118, 1184
Alw21I	GWGCWC	1 cut(s)	465
Alw26I	GTCTC	2 cut(s)	340, 1000
AlwI	GGATC	3 cut(s)	146, 159, 1363
AlwNI	CAGNNNCTG	1 cut(s)	147
Ama87I	CYCGRG	1 cut(s)	77
AoxI	GGCC	4 cut(s)	175, 673, 696, 789
ApeKI	GCWGC	4 cut(s)	261, 312, 863, 1304
ApoI	RAATTY	2 cut(s)	744, 1259
Asp700I	GAANNNNTTC	2 cut(s)	745, 1046
AspS9I	GGNCC	3 cut(s)	674, 1479, 1494
AsuHPI	GGTGA	1 cut(s)	154
AsuNHI	GCTAGC	1 cut(s)	391
AvaI	CYCGRG	1 cut(s)	77
AvaII	GGWCC	2 cut(s)	1479, 1494
BamHI	GGATCC	1 cut(s)	151
BanII	GRGCYC	1 cut(s)	84
BarI	GAAGNNNNNNTAC	2 cut(s)	479, 511
Bbv12I	GWGCWC	1 cut(s)	465
BbvI	GCAGC	4 cut(s)	248, 324, 875, 1291
BccI	CCATC	4 cut(s)	56, 62, 800, 1247
BceAI	ACGGC	1 cut(s)	911
BcgI	CGANNNNNNTGC	2 cut(s)	228, 262
BciT130I	CCWGG	3 cut(s)	174, 219, 1491
BclI	TGATCA	1 cut(s)	159
BcoDI	GTCTC	2 cut(s)	340, 1000
BfaI	CTAG	7 cut(s)	194, 392, 476, 738, 854, 905, 1248
BfmI	CTRYAG	1 cut(s)	1305
BglII	AGATCT	1 cut(s)	948
BisI	GCNGC	5 cut(s)	262, 313, 456, 864, 1305
BlsI	GCNGC	5 cut(s)	263, 314, 457, 865, 1306
BmcAI	AGTACT	2 cut(s)	125, 497
Bme1390I	CCNGG	3 cut(s)	174, 219, 1491
Bme18I	GGWCC	2 cut(s)	1479, 1494
BmeT110I	CYCGRG	1 cut(s)	77
BmgT120I	GGNCC	3 cut(s)	674, 1479, 1494
BmiI	GGNNCC	6 cut(s)	153, 987, 1317, 1481, 1495, 1496
BmrFI	CCNGG	3 cut(s)	174, 219, 1491
BmsI	GCATC	4 cut(s)	481, 549, 703, 907
BmtI	GCTAGC	1 cut(s)	395
BplI	GAGNNNNNCTC	2 cut(s)	337, 369
BpuEI	CTTGAG	3 cut(s)	96, 364, 703
BsaI	GGTCTC	1 cut(s)	340
BsaJI	CCNNGG	1 cut(s)	1490
BsaXI	ACNNNNNCTCC	2 cut(s)	154, 184
Bsc4I	CCNNNNNNNGG	2 cut(s)	882, 982
Bse118I	RCCGGY	1 cut(s)	349
Bse1I	ACTGG	1 cut(s)	503
Bse3DI	GCAATG	3 cut(s)	509, 583, 1080
BseBI	CCWGG	3 cut(s)	174, 219, 1491
BseDI	CCNNGG	1 cut(s)	1490
BseGI	GGATG	6 cut(s)	54, 97, 564, 718, 1120, 1209
BseLI	CCNNNNNNNGG	2 cut(s)	882, 982
BseMI	GCAATG	3 cut(s)	509, 583, 1080
BseNI	ACTGG	1 cut(s)	503
BseRI	GAGGAG	3 cut(s)	159, 924, 1196
BseXI	GCAGC	4 cut(s)	248, 324, 875, 1291
BseYI	CCCAGC	2 cut(s)	147, 1497
Bsh1236I	CGCG	1 cut(s)	383
BshFI	GGCC	4 cut(s)	177, 675, 698, 791
BsiHKAI	GWGCWC	1 cut(s)	465
BsiHKCI	CYCGRG	1 cut(s)	77
BsiSI	CCGG	1 cut(s)	350
BslFI	GGGAC	2 cut(s)	1465, 1507
BslI	CCNNNNNNNGG	2 cut(s)	882, 982
BsmAI	GTCTC	2 cut(s)	340, 1000
BsmFI	GGGAC	2 cut(s)	1465, 1507
BsnI	GGCC	4 cut(s)	177, 675, 698, 791
Bso31I	GGTCTC	1 cut(s)	340
BsoBI	CYCGRG	1 cut(s)	77
Bsp1286I	GDGCHC	2 cut(s)	84, 465
Bsp1407I	TGTACA	1 cut(s)	1422
Bsp143I	GATC	4 cut(s)	151, 159, 948, 1368
BspACI	CCGC	7 cut(s)	383, 455, 691, 1168, 1320, 1325, 1501
BspANI	GGCC	4 cut(s)	177, 675, 698, 791
BspFNI	CGCG	1 cut(s)	383
BspLI	GGNNCC	6 cut(s)	153, 987, 1317, 1481, 1495, 1496
BspMAI	CTGCAG	1 cut(s)	1309
BspOI	GCTAGC	1 cut(s)	395
BspPI	GGATC	3 cut(s)	146, 159, 1363
BspQI	GCTCTTC	1 cut(s)	361
BspTNI	GGTCTC	1 cut(s)	340
BsrDI	GCAATG	3 cut(s)	509, 583, 1080
BsrFI	RCCGGY	1 cut(s)	349
BsrGI	TGTACA	1 cut(s)	1422
BsrI	ACTGG	1 cut(s)	503
BssAI	RCCGGY	1 cut(s)	349
BssECI	CCNNGG	1 cut(s)	1490
BssMI	GATC	4 cut(s)	151, 159, 948, 1368
Bst2UI	CCWGG	3 cut(s)	174, 219, 1491
Bst4CI	ACNGT	7 cut(s)	16, 123, 128, 664, 1015, 1422, 1432
Bst6I	CTCTTC	3 cut(s)	361, 411, 756
BstAPI	GCANNNNNTGC	2 cut(s)	917, 1082
BstAUI	TGTACA	1 cut(s)	1422
BstC8I	GCNNGC	6 cut(s)	393, 453, 465, 700, 1087, 1284
BstF5I	GGATG	6 cut(s)	54, 97, 564, 718, 1120, 1209
BstFNI	CGCG	1 cut(s)	383
BstKTI	GATC	4 cut(s)	154, 162, 951, 1371
BstMAI	GTCTC	2 cut(s)	340, 1000
BstMBI	GATC	4 cut(s)	151, 159, 948, 1368
BstMWI	GCNNNNNNNGC	7 cut(s)	203, 464, 469, 872, 917, 1082, 1304
BstNI	CCWGG	3 cut(s)	174, 219, 1491
BstNSI	RCATGY	1 cut(s)	226
BstSCI	CCNGG	3 cut(s)	172, 217, 1489
BstSFI	CTRYAG	1 cut(s)	1305
BstUI	CGCG	1 cut(s)	383
BstV1I	GCAGC	4 cut(s)	248, 324, 875, 1291
BstX2I	RGATCY	2 cut(s)	151, 948
BstXI	CCANNNNNNTGG	2 cut(s)	807, 1476
BstYI	RGATCY	2 cut(s)	151, 948
BsuRI	GGCC	4 cut(s)	177, 675, 698, 791
BtgZI	GCGATG	1 cut(s)	1078
BtsCI	GGATG	6 cut(s)	54, 97, 564, 718, 1120, 1209
BtsI	GCAGTG	1 cut(s)	465
BtsIMutI	CAGTG	3 cut(s)	465, 660, 714
Cac8I	GCNNGC	6 cut(s)	393, 453, 465, 700, 1087, 1284
CaiI	CAGNNNCTG	1 cut(s)	147
Cfr10I	RCCGGY	1 cut(s)	349
Cfr13I	GGNCC	3 cut(s)	674, 1479, 1494
Csp6I	GTAC	8 cut(s)	124, 181, 400, 496, 1016, 1201, 1347, 1423
CviQI	GTAC	8 cut(s)	124, 181, 400, 496, 1016, 1201, 1347, 1423
DpnI	GATC	4 cut(s)	153, 161, 950, 1370
DpnII	GATC	4 cut(s)	151, 159, 948, 1368
Eam1104I	CTCTTC	3 cut(s)	361, 411, 756
EarI	CTCTTC	3 cut(s)	361, 411, 756
EciI	GGCGGA	1 cut(s)	1183
Eco24I	GRGCYC	1 cut(s)	84
Eco31I	GGTCTC	1 cut(s)	340
Eco47I	GGWCC	2 cut(s)	1479, 1494
Eco57I	CTGAAG	2 cut(s)	276, 690
Eco88I	CYCGRG	1 cut(s)	77
EcoO109I	RGGNCCY	1 cut(s)	1494
EcoRII	CCWGG	3 cut(s)	172, 217, 1489
EcoT22I	ATGCAT	1 cut(s)	474
EcoT38I	GRGCYC	1 cut(s)	84
FalI	AAGNNNNNCTT	2 cut(s)	779, 811
FaqI	GGGAC	2 cut(s)	1465, 1507
FauI	CCCGC	1 cut(s)	1327
FbaI	TGATCA	1 cut(s)	159
Fnu4HI	GCNGC	5 cut(s)	262, 313, 456, 864, 1305
FokI	GGATG	6 cut(s)	41, 104, 571, 725, 1107, 1196
FriOI	GRGCYC	1 cut(s)	84
Fsp4HI	GCNGC	5 cut(s)	262, 313, 456, 864, 1305
FspBI	CTAG	7 cut(s)	194, 392, 476, 738, 854, 905, 1248
GluI	GCNGC	5 cut(s)	262, 313, 456, 864, 1305
GsaI	CCCAGC	2 cut(s)	151, 1501
HaeIII	GGCC	4 cut(s)	177, 675, 698, 791
HapII	CCGG	1 cut(s)	350
HincII	GTYRAC	1 cut(s)	1435
HindII	GTYRAC	1 cut(s)	1435
HinfI	GANTC	1 cut(s)	274
HpaII	CCGG	1 cut(s)	350
HphI	GGTGA	1 cut(s)	154
Hpy166II	GTNNAC	2 cut(s)	1018, 1435
Hpy188I	TCNGA	4 cut(s)	143, 256, 1065, 1266
Hpy188III	TCNNGA	5 cut(s)	77, 356, 733, 776, 1366
Hpy8I	GTNNAC	2 cut(s)	1018, 1435
Hpy99I	CGWCG	1 cut(s)	25
HpyAV	CCTTC	5 cut(s)	251, 663, 688, 1066, 1165
HpyCH4III	ACNGT	7 cut(s)	16, 123, 128, 664, 1015, 1422, 1432
HpyCH4V	TGCA	8 cut(s)	312, 472, 509, 576, 762, 911, 944, 1307
HpyF10VI	GCNNNNNNNGC	7 cut(s)	203, 464, 469, 872, 917, 1082, 1304
KflI	GGGWCCC	1 cut(s)	1494
Ksp22I	TGATCA	1 cut(s)	159
Kzo9I	GATC	4 cut(s)	151, 159, 948, 1368
LguI	GCTCTTC	1 cut(s)	361
LmnI	GCTCC	2 cut(s)	269, 991
Lsp1109I	GCAGC	4 cut(s)	248, 324, 875, 1291
LweI	GCATC	4 cut(s)	481, 549, 703, 907
MaeI	CTAG	7 cut(s)	194, 392, 476, 738, 854, 905, 1248
MaeIII	GTNAC	2 cut(s)	664, 1408
MalI	GATC	4 cut(s)	153, 161, 950, 1370
MboI	GATC	4 cut(s)	151, 159, 948, 1368
MflI	RGATCY	2 cut(s)	151, 948
MhlI	GDGCHC	2 cut(s)	84, 465
MluCI	AATT	8 cut(s)	42, 136, 627, 744, 764, 783, 803, 1259
MmeI	TCCRAC	1 cut(s)	801
MnlI	CCTC	9 cut(s)	68, 120, 180, 414, 574, 945, 1214, 1217, 1260
Mph1103I	ATGCAT	1 cut(s)	474
MroXI	GAANNNNTTC	2 cut(s)	745, 1046
MseI	TTAA	2 cut(s)	728, 786
MslI	CAYNNNNRTG	2 cut(s)	707, 1448
MspA1I	CMGCKG	2 cut(s)	1327, 1501
MspI	CCGG	1 cut(s)	350
MspR9I	CCNGG	3 cut(s)	174, 219, 1491
MvaI	CCWGG	3 cut(s)	174, 219, 1491
MvnI	CGCG	1 cut(s)	383
MwoI	GCNNNNNNNGC	7 cut(s)	203, 464, 469, 872, 917, 1082, 1304
NdeII	GATC	4 cut(s)	151, 159, 948, 1368
NheI	GCTAGC	1 cut(s)	391
NlaIV	GGNNCC	6 cut(s)	153, 987, 1317, 1481, 1495, 1496
NmuCI	GTSAC	1 cut(s)	1408
NsiI	ATGCAT	1 cut(s)	474
NspI	RCATGY	1 cut(s)	226
OliI	CACNNNNGTG	1 cut(s)	707
PciSI	GCTCTTC	1 cut(s)	361
PdmI	GAANNNNTTC	2 cut(s)	745, 1046
PfeI	GAWTC	1 cut(s)	274
PkrI	GCNGC	5 cut(s)	263, 314, 457, 865, 1306
PpuMI	RGGWCCY	1 cut(s)	1494
PsiI	TTATAA	1 cut(s)	587
Psp5II	RGGWCCY	1 cut(s)	1494
Psp6I	CCWGG	3 cut(s)	172, 217, 1489
PspFI	CCCAGC	2 cut(s)	147, 1497
PspGI	CCWGG	3 cut(s)	172, 217, 1489
PspN4I	GGNNCC	6 cut(s)	153, 987, 1317, 1481, 1495, 1496
PspPI	GGNCC	3 cut(s)	674, 1479, 1494
PspPPI	RGGWCCY	1 cut(s)	1494
PstI	CTGCAG	1 cut(s)	1309
PstNI	CAGNNNCTG	1 cut(s)	147
PsuI	RGATCY	2 cut(s)	151, 948
RsaI	GTAC	8 cut(s)	125, 182, 401, 497, 1017, 1202, 1348, 1424
RsaNI	GTAC	8 cut(s)	124, 181, 400, 496, 1016, 1201, 1347, 1423
RseI	CAYNNNNRTG	2 cut(s)	707, 1448
SapI	GCTCTTC	1 cut(s)	361
SaqAI	TTAA	2 cut(s)	728, 786
SatI	GCNGC	5 cut(s)	262, 313, 456, 864, 1305
Sau3AI	GATC	4 cut(s)	151, 159, 948, 1368
Sau96I	GGNCC	3 cut(s)	674, 1479, 1494
ScaI	AGTACT	2 cut(s)	125, 497
ScrFI	CCNGG	3 cut(s)	174, 219, 1491
SduI	GDGCHC	2 cut(s)	84, 465
SfaNI	GCATC	4 cut(s)	481, 549, 703, 907
SfcI	CTRYAG	1 cut(s)	1305
SinI	GGWCC	2 cut(s)	1479, 1494
SmiMI	CAYNNNNRTG	2 cut(s)	707, 1448
SmlI	CTYRAG	3 cut(s)	111, 343, 682
SmoI	CTYRAG	3 cut(s)	111, 343, 682
Sse9I	AATT	8 cut(s)	42, 136, 627, 744, 764, 783, 803, 1259
SsiI	CCGC	7 cut(s)	383, 455, 691, 1168, 1320, 1325, 1501
SspI	AATATT	2 cut(s)	550, 592
SspMI	CTAG	7 cut(s)	194, 392, 476, 738, 854, 905, 1248
StyD4I	CCNGG	3 cut(s)	172, 217, 1489
TaaI	ACNGT	7 cut(s)	16, 123, 128, 664, 1015, 1422, 1432
TaqI	TCGA	3 cut(s)	734, 1396, 1465
TasI	AATT	8 cut(s)	42, 136, 627, 744, 764, 783, 803, 1259
TatI	WGTACW	4 cut(s)	123, 180, 495, 1422
TauI	GCSGC	1 cut(s)	458
TfiI	GAWTC	1 cut(s)	274
Tru1I	TTAA	2 cut(s)	728, 786
Tru9I	TTAA	2 cut(s)	728, 786
TscAI	CASTG	3 cut(s)	465, 667, 714
TseFI	GTSAC	1 cut(s)	1408
TseI	GCWGC	4 cut(s)	261, 312, 863, 1304
Tsp45I	GTSAC	1 cut(s)	1408
TspDTI	ATGAA	7 cut(s)	375, 398, 795, 830, 1081, 1211, 1218
TspRI	CASTG	3 cut(s)	465, 667, 714
VpaK11BI	GGWCC	2 cut(s)	1479, 1494
XapI	RAATTY	2 cut(s)	744, 1259
XceI	RCATGY	1 cut(s)	226
XcmI	CCANNNNNNNNNTGG	1 cut(s)	706
XmnI	GAANNNNTTC	2 cut(s)	745, 1046
XspI	CTAG	7 cut(s)	194, 392, 476, 738, 854, 905, 1248
ZrmI	AGTACT	2 cut(s)	125, 497
Zsp2I	ATGCAT	1 cut(s)	474

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.