MD04G1128800.v1.1

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011147
Physical Location & Seq
Forward (+)
21473029 .. 21476341
3313 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1128800.v1.1.491

Sequence Viewer

Length: 2619 bp
ATGGGAAAGAATTTGAGAGACAAAAACCCTAACAATTCATCAGGGAATGAGCATTCACCAGGGTGCGTTTGGGGATTACTTCACATGATGAAGTATCACCACTGGCATTATGTCAAGAAAAGACTTGTGCACAAGAGGCATCGCGGCAATAAACATGCTGTTGGAGTTGGGATTCCGGGAAGCTGTACTGGAAATGCCTGTATGCCAGAAGACACTGATGCTAAGAGTGACAAATCTAACGGTGAAGAAAGGACGAGGCAGTCCATTCCAGCAGGCATACATTCCGTCAAGGCCCGAATAAAAGCACGGATTGCTGGAGAAATGTCAAAGAAGGGTAGACATAACCGGGCTTCAAGCTGTCCTGTACGATCACAATTGAAGCGAACAGAATCCATGCATCGTTTGGAACCACCACCTAACCTGTACCCGATTGCTGAAATGGTATTGAATGAGAATAGTCCTAGAATTGTTCACCACGGCAATGAAAAATCCTGTGCTACAAGCAATACAAGCAATCTGGCATCACTGTCCGCGACAACATATGAACGACCAATTAGTGGCAATAATGATTGTTGTGGAGATAGTTGCCCGCTAATCACTAGAGATCGTACCGTGCATGACAAGGTTAATGAGAATAAAAAAGATCACACATTTATAGAAGAAAAGATGGATGATGACAGAATGCAAGATATGTTCGAGAAGAAGTTGATACATGTAAAAGAACTCGATGCAGATGCTTCCCTACACCATCCAAAGGAGTATTTGGAGGCGTTGGATATAATCAATGTGAACAAGGAGTTATTAGTAAAGATTTTACATGATCCCGGCTCTCCTTTAGCTGCGCACTTCCATAATCAGCAAGCCGTCAGTGCAAAAATGGGTTTAACTAAGTCAGAGTCATTCCCTGTCAAGCTCAGGCACAAGCATGAATTGACAGGGTCTCCTTTAATAGAGGAAAGCAAGTCGAAAAACAGTATTAAAACAAAGAAATTAGCAGGGTCATCTTCTTTGAAATATGCTGGTGAACGCTCAATTCCATCGATCATTGAGCACAATGCAGATGGGATTCAAAAGCTAAACCAAACAATTGCCGATAGTTCTGCTTCTGGTACTTCCGAAAGTTTGAGGAACCAAGGTGAGAATCGCATGGCAATTAAACGGTTCAAGGATCTGAGACAAAAAATAAAGCATGTAATCAAGGAGAGCAGACAAGAGAGACATATGATTGCCATGGATGCCATCCTTCACAAGGTTCCTCATGGCCAAAAGCTTACTAAAGAAATGGAGCAAGAGATCATTAACCATTCGAAGGACCCTGCAATGAACAGAGAAGGTAAAGACAGTCCAGCAAGCAGTTATGATAGCGATCATTCGGTTTCTACCATCAAGAAAAAGCAACAGCACCACATAAGAAGAGCGTCATCACTCAATGCATCGCTAGATAAGTACTGTGAGTTGTACGAGACTAGTTGCAACAGAGAAGCCAAATGCCAAACAACATCCGAGAGATTGAAGTTGAGAAATGAAAGAGTAGGCTCACCATTACGTTATGTTCCAAAAACGCTGGGGAGGATTTTTTCGTTACCTGAAATTAAATCATATAATTATCAGAACGAGGAATCTTCTGATGTGTTTTCTTCTGGAGCTCCAATTAGGGCTGTTGTGGATGGAAATGCAAGCAGAAGAAGTAGCTTTGATGAACAAAATAGTGTACACCATAGTATAGGTTCAGAAAATCATACGGAGCTAGATGCGCCTGTAGAAAGTGAGATTAAAAATTTGGCTGGCGGTGGTGAATTCAAGGCAGTTTCCAGAGATAAGGTGGCATCAACATCAATTGTAAACAGGGAGAAAAGTGCTCCAGTGGATTCAATTGTTAACGATATTGAAAGCTTGACAGAACCATTTCCAATCTCTGTGCCCGAGTTCAACGTTTCCCTCTCTGAAGCAGAAATGGAAATAAAACCTCAAGATGGAATAGAAAGCTTGGCCAAGCTAGAGCATGATTCGCCTAATGATACACCTAAGCCGGTTCACATGGATACGGAGAAGATAGGAAGTCCAATGAAGCACTTGAGGTACGACATGACTCAGGTCCGCATTGATGAAAGAGACTTGGCCGATTTCAATTATGTGCGAGATGTGTTGGAGCAATCCAGATTCAGTGGAAATGAGTCCCTCGGGACATGGCATTTGGATGAGCAGCCTGTTGATCCTTTGATGTATGGTGACACTGAGGGCTGCTTGGTGCATGATCCTGATTGTTCAGGAAACGAAGAAGGCGGGAAATGTGATCACTATCTTCTGTTTGATCTGATCAATGAGGTGCTGATGGAGATATATGGGAGATCATACAACTACTGTCCGGTGGCGTTGTCTTCCTTATGTCACATTCCTCTAATGCCGGCAGGGCACCATGTTTTGAAGGAAGTTTGGGCCCTTGTGAGTTGGAACTTGAGCTTGAGACCTGAGGTTGATCAATCACTGGATTATGCTCTGAGTCGAGACCTGGAGAAGAGCGACGGATGGATGAACCTCCAGTTTGATTCTGAGTGCATAGGGATTGAGCTGGAGGACTTAATTTTTGATGAACTTTTAGAGGAAGTTCTCTGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3741 DUF4378

Protein Analysis

873

Amino Acids

98.01

Weight (kDa)

6.24

Isoelectric Point (pI)

55.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3741 PF12552 234 - 277 7.2e-15 Protein of unknown function (DUF3741)
DUF4378 PF14309 708 - 868 1.1e-38 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02390 AT5G02390 AT5G02390
fragaria_vesca FvH4_6g13720
malus_domestica MD04G1128800.v1.1 MD12G1141400.v1.1
prunus_persica Prupe.6G254700_v2.0.a1
pyrus_communis pycom04g11700 pycom12g13630
rosa_chinensis RchiOBHm_Chr3g0466591
rosa_laevigata RLG00000024561
rosa_multiflora Rmu_sc0020778.1_g000002
rosa_roxburghii Rroxscaffold_6G00414320
rosa_rugosa Rorug03G0083000.1 Rorug03G0083100
rosa_samantha Rh3AG129900 Rh3BG151300 Rh3CG151000 Rh3DG151900
rosa_wichuraiana Rw3G012280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 259
Acc16I TGCGCA 1 cut(s) 843
AccB1I GGYRCC 1 cut(s) 2412
AccB7I CCANNNNNTGG 1 cut(s) 557
AccI GTMKAC 1 cut(s) 337
AccII CGCG 2 cut(s) 144, 533
AciI CCGC 6 cut(s) 144, 531, 590, 1788, 2098, 2283
AclI AACGTT 1 cut(s) 1932
AclWI GGATC 4 cut(s) 815, 1176, 2207, 2249
AcoI YGGCCR 3 cut(s) 1261, 1989, 2118
AcsI RAATTY 3 cut(s) 10, 1777, 1796
AcuI CTGAAG 1 cut(s) 1965
AfaI GTAC 9 cut(s) 187, 366, 425, 610, 1111, 1448, 1460, 1713, 2081
AfiI CCNNNNNNNGG 5 cut(s) 557, 754, 1249, 1309, 1973
AflIII ACRYGT 1 cut(s) 712
AhlI ACTAGT 1 cut(s) 1466
AjnI CCWGG 2 cut(s) 58, 2508
AleI CACNNNNGTG 1 cut(s) 61
Alw21I GWGCWC 4 cut(s) 132, 1053, 1648, 1861
Alw26I GTCTC 8 cut(s) 12, 945, 1168, 1210, 1457, 2106, 2458, 2499
Alw44I GTGCAC 1 cut(s) 128
AlwI GGATC 4 cut(s) 815, 1176, 2207, 2249
Ama87I CYCGRG 2 cut(s) 1922, 2180
AoxI GGCC 5 cut(s) 291, 1261, 1989, 2118, 2436
ApaI GGGCCC 1 cut(s) 2440
ApaLI GTGCAC 1 cut(s) 128
ApeKI GCWGC 3 cut(s) 839, 2203, 2241
ApoI RAATTY 3 cut(s) 10, 1777, 1796
ArsI GACNNNNNNTTYG 4 cut(s) 2176, 2208, 2567, 2599
Asp700I GAANNNNTTC 1 cut(s) 1905
AspLEI GCGC 2 cut(s) 844, 1756
AspS9I GGNCC 5 cut(s) 292, 1312, 2095, 2436, 2437
AsuC2I CCSGG 3 cut(s) 177, 347, 825
AsuHPI GGTGA 9 cut(s) 48, 89, 254, 464, 1034, 1148, 1530, 1805, 2240
AsuII TTCGAA 1 cut(s) 1307
AvaI CYCGRG 2 cut(s) 1922, 2180
AvaII GGWCC 2 cut(s) 1312, 2095
AxyI CCTNAGG 1 cut(s) 2469
BaeGI GKGCMC 4 cut(s) 132, 1923, 2415, 2440
BalI TGGCCA 2 cut(s) 1263, 1991
BanI GGYRCC 1 cut(s) 2412
BanII GRGCYC 2 cut(s) 1648, 2440
BbsI GAAGAC 2 cut(s) 216, 2370
Bbv12I GWGCWC 4 cut(s) 132, 1053, 1648, 1861
BbvI GCAGC 3 cut(s) 826, 2215, 2228
BceAI ACGGC 2 cut(s) 493, 848
BcgI CGANNNNNNTGC 4 cut(s) 716, 750, 1082, 1116
BciT130I CCWGG 2 cut(s) 60, 2510
BciVI GTATCC 1 cut(s) 2035
BclI TGATCA 3 cut(s) 2293, 2316, 2476
BcnI CCSGG 3 cut(s) 177, 347, 825
BcoDI GTCTC 8 cut(s) 12, 945, 1168, 1210, 1457, 2106, 2458, 2499
BcuI ACTAGT 1 cut(s) 1466
BfaI CTAG 6 cut(s) 462, 600, 1439, 1467, 1748, 1997
BfmI CTRYAG 1 cut(s) 1758
BfuI GTATCC 1 cut(s) 2035
BglI GCCNNNNNGGC 1 cut(s) 2410
BisI GCNGC 4 cut(s) 145, 840, 2204, 2242
BlsI GCNGC 4 cut(s) 146, 841, 2205, 2243
BmcAI AGTACT 1 cut(s) 1448
Bme1390I CCNGG 5 cut(s) 60, 177, 347, 825, 2510
Bme18I GGWCC 2 cut(s) 1312, 2095
BmeT110I CYCGRG 2 cut(s) 1922, 2180
BmgT120I GGNCC 5 cut(s) 292, 1312, 2095, 2436, 2437
BmiI GGNNCC 6 cut(s) 408, 1130, 1254, 1314, 2414, 2438
BmrFI CCNGG 5 cut(s) 60, 177, 347, 825, 2510
BoxI GACNNNNGTC 1 cut(s) 2093
BpiI GAAGAC 2 cut(s) 216, 2370
BpmI CTGGAG 6 cut(s) 336, 1662, 1845, 2522, 2531, 2591
Bpu10I CCTNAGC 2 cut(s) 914, 2025
Bpu14I TTCGAA 1 cut(s) 1307
BpuEI CTTGAG 4 cut(s) 1953, 2095, 2476, 2482
BpuMI CCSGG 3 cut(s) 177, 347, 825
Bsa29I ATCGAT 1 cut(s) 1040
BsaBI GATNNNNATC 2 cut(s) 1365, 2298
BsaI GGTCTC 3 cut(s) 945, 2458, 2499
BsaJI CCNNGG 5 cut(s) 59, 475, 1132, 1230, 2179
BsaWI WCCGGW 1 cut(s) 2365
BsaXI ACNNNNNCTCC 2 cut(s) 925, 955
Bsc4I CCNNNNNNNGG 5 cut(s) 557, 754, 1249, 1309, 1973
Bse118I RCCGGY 2 cut(s) 2029, 2404
Bse1I ACTGG 5 cut(s) 107, 193, 1862, 2490, 2539
Bse21I CCTNAGG 1 cut(s) 2469
Bse3DI GCAATG 2 cut(s) 487, 1326
Bse8I GATNNNNATC 2 cut(s) 1365, 2298
BseBI CCWGG 2 cut(s) 60, 2510
BseCI ATCGAT 1 cut(s) 1040
BseDI CCNNGG 5 cut(s) 59, 475, 1132, 1230, 2179
BseGI GGATG 9 cut(s) 676, 748, 1239, 1240, 1499, 1672, 2203, 2531, 2535
BseJI GATNNNNATC 2 cut(s) 1365, 2298
BseLI CCNNNNNNNGG 5 cut(s) 557, 754, 1249, 1309, 1973
BseMI GCAATG 2 cut(s) 487, 1326
BseMII CTCAG 7 cut(s) 928, 1163, 2105, 2226, 2460, 2489, 2541
BseNI ACTGG 5 cut(s) 107, 193, 1862, 2490, 2539
BseSI GKGCMC 4 cut(s) 132, 1923, 2415, 2440
BseXI GCAGC 3 cut(s) 826, 2215, 2228
BseYI CCCAGC 1 cut(s) 1564
Bsh1236I CGCG 2 cut(s) 144, 533
BshFI GGCC 5 cut(s) 293, 1263, 1991, 2120, 2438
BshNI GGYRCC 1 cut(s) 2412
BshVI ATCGAT 1 cut(s) 1040
BsiHKAI GWGCWC 4 cut(s) 132, 1053, 1648, 1861
BsiHKCI CYCGRG 2 cut(s) 1922, 2180
BsiSI CCGG 6 cut(s) 176, 346, 825, 2030, 2366, 2405
BslFI GGGAC 2 cut(s) 2161, 2197
BslI CCNNNNNNNGG 5 cut(s) 557, 754, 1249, 1309, 1973
BsmAI GTCTC 8 cut(s) 12, 945, 1168, 1210, 1457, 2106, 2458, 2499
BsmFI GGGAC 2 cut(s) 2161, 2197
BsmI GAATGC 2 cut(s) 52, 687
BsnI GGCC 5 cut(s) 293, 1263, 1991, 2120, 2438
Bso31I GGTCTC 3 cut(s) 945, 2458, 2499
BsoBI CYCGRG 2 cut(s) 1922, 2180
Bsp119I TTCGAA 1 cut(s) 1307
Bsp120I GGGCCC 1 cut(s) 2436
Bsp1286I GDGCHC 7 cut(s) 132, 1053, 1648, 1861, 1923, 2415, 2440
Bsp1407I TGTACA 1 cut(s) 1711
Bsp19I CCATGG 1 cut(s) 1230
BspACI CCGC 6 cut(s) 144, 531, 590, 1788, 2098, 2283
BspANI GGCC 5 cut(s) 293, 1263, 1991, 2120, 2438
BspCNI CTCAG 7 cut(s) 927, 1164, 2104, 2227, 2461, 2490, 2542
BspDI ATCGAT 1 cut(s) 1040
BspFNI CGCG 2 cut(s) 144, 533
BspLI GGNNCC 6 cut(s) 408, 1130, 1254, 1314, 2414, 2438
BspPI GGATC 4 cut(s) 815, 1176, 2207, 2249
BspQI GCTCTTC 2 cut(s) 1408, 2510
BspT104I TTCGAA 1 cut(s) 1307
BspT107I GGYRCC 1 cut(s) 2412
BspTNI GGTCTC 3 cut(s) 945, 2458, 2499
BsrDI GCAATG 2 cut(s) 487, 1326
BsrFI RCCGGY 2 cut(s) 2029, 2404
BsrGI TGTACA 1 cut(s) 1711
BsrI ACTGG 5 cut(s) 107, 193, 1862, 2490, 2539
BssAI RCCGGY 2 cut(s) 2029, 2404
BssECI CCNNGG 5 cut(s) 59, 475, 1132, 1230, 2179
BssT1I CCWWGG 2 cut(s) 1132, 1230
Bst2UI CCWGG 2 cut(s) 60, 2510
Bst4CI ACNGT 8 cut(s) 242, 528, 613, 974, 1161, 1343, 1451, 2363
Bst6I CTCTTC 2 cut(s) 1408, 2510
BstAPI GCANNNNNTGC 1 cut(s) 311
BstAUI TGTACA 1 cut(s) 1711
BstBI TTCGAA 1 cut(s) 1307
BstC8I GCNNGC 7 cut(s) 274, 590, 861, 1351, 1678, 1786, 2406
BstDSI CCRYGG 2 cut(s) 475, 1230
BstENI CCTNNNNNAGG 1 cut(s) 1247
BstF5I GGATG 9 cut(s) 676, 748, 1239, 1240, 1499, 1672, 2203, 2531, 2535
BstFNI CGCG 2 cut(s) 144, 533
BstHHI GCGC 2 cut(s) 844, 1756
BstMAI GTCTC 8 cut(s) 12, 945, 1168, 1210, 1457, 2106, 2458, 2499
BstMWI GCNNNNNNNGC 8 cut(s) 136, 311, 510, 869, 1235, 1753, 2008, 2410
BstNI CCWGG 2 cut(s) 60, 2510
BstNSI RCATGY 3 cut(s) 158, 716, 1193
BstPAI GACNNNNGTC 1 cut(s) 2093
BstSCI CCNGG 5 cut(s) 58, 175, 345, 823, 2508
BstSFI CTRYAG 1 cut(s) 1758
BstSLI GKGCMC 4 cut(s) 132, 1923, 2415, 2440
BstUI CGCG 2 cut(s) 144, 533
BstV1I GCAGC 3 cut(s) 826, 2215, 2228
BstV2I GAAGAC 2 cut(s) 216, 2370
BstX2I RGATCY 1 cut(s) 1168
BstYI RGATCY 1 cut(s) 1168
Bsu15I ATCGAT 1 cut(s) 1040
Bsu36I CCTNAGG 1 cut(s) 2469
BsuI GTATCC 1 cut(s) 2035
BsuRI GGCC 5 cut(s) 293, 1263, 1991, 2120, 2438
BsuTUI ATCGAT 1 cut(s) 1040
BtgI CCRYGG 2 cut(s) 475, 1230
BtgZI GCGATG 2 cut(s) 125, 1419
BtsCI GGATG 9 cut(s) 676, 748, 1239, 1240, 1499, 1672, 2203, 2531, 2535
BtsIMutI CAGTG 8 cut(s) 100, 213, 524, 874, 1869, 2170, 2232, 2483
Cac8I GCNNGC 7 cut(s) 274, 590, 861, 1351, 1678, 1786, 2406
CfoI GCGC 2 cut(s) 844, 1756
Cfr10I RCCGGY 2 cut(s) 2029, 2404
Cfr13I GGNCC 5 cut(s) 292, 1312, 2095, 2436, 2437
ClaI ATCGAT 1 cut(s) 1040
CseI GACGC 1 cut(s) 1407
Csp6I GTAC 9 cut(s) 186, 365, 424, 609, 1110, 1447, 1459, 1712, 2080
CviQI GTAC 9 cut(s) 186, 365, 424, 609, 1110, 1447, 1459, 1712, 2080
DrdI GACNNNNNNGTC 1 cut(s) 259
DseDI GACNNNNNNGTC 1 cut(s) 259
EaeI YGGCCR 3 cut(s) 1261, 1989, 2118
Eam1104I CTCTTC 2 cut(s) 1408, 2510
EarI CTCTTC 2 cut(s) 1408, 2510
Ecl136II GAGCTC 1 cut(s) 1646
Eco130I CCWWGG 2 cut(s) 1132, 1230
Eco24I GRGCYC 2 cut(s) 1648, 2440
Eco31I GGTCTC 3 cut(s) 945, 2458, 2499
Eco47I GGWCC 2 cut(s) 1312, 2095
Eco53kI GAGCTC 1 cut(s) 1646
Eco57I CTGAAG 1 cut(s) 1965
Eco81I CCTNAGG 1 cut(s) 2469
Eco88I CYCGRG 2 cut(s) 1922, 2180
EcoICRI GAGCTC 1 cut(s) 1646
EcoNI CCTNNNNNAGG 1 cut(s) 1247
EcoO109I RGGNCCY 2 cut(s) 1312, 2437
EcoRI GAATTC 1 cut(s) 1796
EcoRII CCWGG 2 cut(s) 58, 2508
EcoT14I CCWWGG 2 cut(s) 1132, 1230
EcoT22I ATGCAT 2 cut(s) 399, 1435
EcoT38I GRGCYC 2 cut(s) 1648, 2440
ErhI CCWWGG 2 cut(s) 1132, 1230
FaqI GGGAC 2 cut(s) 2161, 2197
FauI CCCGC 2 cut(s) 597, 2276
FauNDI CATATG 2 cut(s) 541, 1221
FbaI TGATCA 3 cut(s) 2293, 2316, 2476
FblI GTMKAC 1 cut(s) 337
Fnu4HI GCNGC 4 cut(s) 145, 840, 2204, 2242
FokI GGATG 9 cut(s) 683, 735, 1226, 1247, 1486, 1679, 2210, 2538, 2542
FriOI GRGCYC 2 cut(s) 1648, 2440
Fsp4HI GCNGC 4 cut(s) 145, 840, 2204, 2242
FspBI CTAG 6 cut(s) 462, 600, 1439, 1467, 1748, 1997
FspI TGCGCA 1 cut(s) 843
GlaI GCGC 2 cut(s) 843, 1755
GluI GCNGC 4 cut(s) 145, 840, 2204, 2242
GsaI CCCAGC 1 cut(s) 1568
GsuI CTGGAG 6 cut(s) 336, 1662, 1845, 2522, 2531, 2591
HaeIII GGCC 5 cut(s) 293, 1263, 1991, 2120, 2438
HapII CCGG 6 cut(s) 176, 346, 825, 2030, 2366, 2405
HgaI GACGC 1 cut(s) 1407
HhaI GCGC 2 cut(s) 844, 1756
Hin6I GCGC 2 cut(s) 842, 1754
HinP1I GCGC 2 cut(s) 842, 1754
HincII GTYRAC 1 cut(s) 1879
HindII GTYRAC 1 cut(s) 1879
HindIII AAGCTT 3 cut(s) 1268, 1891, 1984
HpaI GTTAAC 1 cut(s) 1879
HpaII CCGG 6 cut(s) 176, 346, 825, 2030, 2366, 2405
HphI GGTGA 9 cut(s) 48, 89, 254, 464, 1034, 1148, 1530, 1805, 2240
Hpy99I CGWCG 1 cut(s) 2525
HpyAV CCTTC 6 cut(s) 325, 1253, 1303, 1325, 2273, 2419
HpyCH4III ACNGT 8 cut(s) 242, 528, 613, 974, 1161, 1343, 1451, 2363
HpyCH4IV ACGT 2 cut(s) 1546, 1932
HpyF10VI GCNNNNNNNGC 8 cut(s) 136, 311, 510, 869, 1235, 1753, 2008, 2410
HpySE526I ACGT 2 cut(s) 1546, 1932
HspAI GCGC 2 cut(s) 842, 1754
KroI GCCGGC 1 cut(s) 2404
KroNI GCCGGC 1 cut(s) 2406
Ksp22I TGATCA 3 cut(s) 2293, 2316, 2476
KspAI GTTAAC 1 cut(s) 1879
LguI GCTCTTC 2 cut(s) 1408, 2510
LmnI GCTCC 6 cut(s) 1285, 1643, 1651, 1744, 1864, 2149
Lsp1109I GCAGC 3 cut(s) 826, 2215, 2228
MaeI CTAG 6 cut(s) 462, 600, 1439, 1467, 1748, 1997
MaeII ACGT 2 cut(s) 1546, 1932
MaeIII GTNAC 4 cut(s) 227, 1581, 2228, 2387
MfeI CAATTG 4 cut(s) 374, 1086, 1836, 1872
MflI RGATCY 1 cut(s) 1168
MhlI GDGCHC 7 cut(s) 132, 1053, 1648, 1861, 1923, 2415, 2440
MlsI TGGCCA 2 cut(s) 1263, 1991
MluNI TGGCCA 2 cut(s) 1263, 1991
MlyI GAGTC 4 cut(s) 905, 2083, 2183, 2509
MmeI TCCRAC 4 cut(s) 142, 753, 2127, 2429
Mox20I TGGCCA 2 cut(s) 1263, 1991
Mph1103I ATGCAT 2 cut(s) 399, 1435
MroNI GCCGGC 1 cut(s) 2404
MroXI GAANNNNTTC 1 cut(s) 1905
MscI TGGCCA 2 cut(s) 1263, 1991
MslI CAYNNNNRTG 4 cut(s) 61, 480, 924, 2196
Msp20I TGGCCA 2 cut(s) 1263, 1991
MspI CCGG 6 cut(s) 176, 346, 825, 2030, 2366, 2405
MspR9I CCNGG 5 cut(s) 60, 177, 347, 825, 2510
MunI CAATTG 4 cut(s) 374, 1086, 1836, 1872
Mva1269I GAATGC 2 cut(s) 52, 687
MvaI CCWGG 2 cut(s) 60, 2510
MvnI CGCG 2 cut(s) 144, 533
MwoI GCNNNNNNNGC 8 cut(s) 136, 311, 510, 869, 1235, 1753, 2008, 2410
NaeI GCCGGC 1 cut(s) 2406
NciI CCSGG 3 cut(s) 177, 347, 825
NcoI CCATGG 1 cut(s) 1230
NdeI CATATG 2 cut(s) 541, 1221
NgoMIV GCCGGC 1 cut(s) 2404
NlaIV GGNNCC 6 cut(s) 408, 1130, 1254, 1314, 2414, 2438
NmuCI GTSAC 3 cut(s) 227, 2228, 2387
NsbI TGCGCA 1 cut(s) 843
NsiI ATGCAT 2 cut(s) 399, 1435
NspI RCATGY 3 cut(s) 158, 716, 1193
NspV TTCGAA 1 cut(s) 1307
OliI CACNNNNGTG 1 cut(s) 61
PciI ACATGT 1 cut(s) 712
PciSI GCTCTTC 2 cut(s) 1408, 2510
PctI GAATGC 2 cut(s) 52, 687
PdiI GCCGGC 1 cut(s) 2406
PdmI GAANNNNTTC 1 cut(s) 1905
PfeI GAWTC 9 cut(s) 172, 389, 1066, 1141, 1619, 1868, 2006, 2160, 2546
PflFI GACNNNGTC 1 cut(s) 937
PflMI CCANNNNNTGG 1 cut(s) 557
PfoI TCCNGGA 1 cut(s) 175
PkrI GCNGC 4 cut(s) 146, 841, 2205, 2243
PleI GAGTC 4 cut(s) 904, 2083, 2182, 2508
PpsI GAGTC 4 cut(s) 904, 2083, 2182, 2508
PpuMI RGGWCCY 1 cut(s) 1312
PscI ACATGT 1 cut(s) 712
PshAI GACNNNNGTC 1 cut(s) 2093
Psp124BI GAGCTC 1 cut(s) 1648
Psp1406I AACGTT 1 cut(s) 1932
Psp5II RGGWCCY 1 cut(s) 1312
Psp6I CCWGG 2 cut(s) 58, 2508
PspFI CCCAGC 1 cut(s) 1564
PspGI CCWGG 2 cut(s) 58, 2508
PspN4I GGNNCC 6 cut(s) 408, 1130, 1254, 1314, 2414, 2438
PspOMI GGGCCC 1 cut(s) 2436
PspPI GGNCC 5 cut(s) 292, 1312, 2095, 2436, 2437
PspPPI RGGWCCY 1 cut(s) 1312
PsuI RGATCY 1 cut(s) 1168
PsyI GACNNNGTC 1 cut(s) 937
RsaI GTAC 9 cut(s) 187, 366, 425, 610, 1111, 1448, 1460, 1713, 2081
RsaNI GTAC 9 cut(s) 186, 365, 424, 609, 1110, 1447, 1459, 1712, 2080
RseI CAYNNNNRTG 4 cut(s) 61, 480, 924, 2196
SacI GAGCTC 1 cut(s) 1648
SapI GCTCTTC 2 cut(s) 1408, 2510
SatI GCNGC 4 cut(s) 145, 840, 2204, 2242
Sau96I GGNCC 5 cut(s) 292, 1312, 2095, 2436, 2437
ScaI AGTACT 1 cut(s) 1448
SchI GAGTC 4 cut(s) 905, 2083, 2183, 2509
ScrFI CCNGG 5 cut(s) 60, 177, 347, 825, 2510
SduI GDGCHC 7 cut(s) 132, 1053, 1648, 1861, 1923, 2415, 2440
SfcI CTRYAG 1 cut(s) 1758
SfuI TTCGAA 1 cut(s) 1307
SinI GGWCC 2 cut(s) 1312, 2095
SmiMI CAYNNNNRTG 4 cut(s) 61, 480, 924, 2196
SmlI CTYRAG 4 cut(s) 1968, 2074, 2455, 2461
SmoI CTYRAG 4 cut(s) 1968, 2074, 2455, 2461
SpeI ACTAGT 1 cut(s) 1466
SsiI CCGC 6 cut(s) 144, 531, 590, 1788, 2098, 2283
SspMI CTAG 6 cut(s) 462, 600, 1439, 1467, 1748, 1997
SstI GAGCTC 1 cut(s) 1648
StyD4I CCNGG 5 cut(s) 58, 175, 345, 823, 2508
StyI CCWWGG 2 cut(s) 1132, 1230
TaaI ACNGT 8 cut(s) 242, 528, 613, 974, 1161, 1343, 1451, 2363
TaiI ACGT 2 cut(s) 1549, 1935
TaqI TCGA 6 cut(s) 696, 726, 965, 1040, 1307, 2503
TatI WGTACW 3 cut(s) 185, 1446, 1711
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 9 cut(s) 172, 389, 1066, 1141, 1619, 1868, 2006, 2160, 2546
TscAI CASTG 8 cut(s) 107, 220, 531, 874, 1869, 2170, 2239, 2490
TseFI GTSAC 3 cut(s) 227, 2228, 2387
TseI GCWGC 3 cut(s) 839, 2203, 2241
Tsp45I GTSAC 3 cut(s) 227, 2228, 2387
TspGWI ACGGA 5 cut(s) 274, 322, 1757, 2060, 2538
TspRI CASTG 8 cut(s) 107, 220, 531, 874, 1869, 2170, 2239, 2490
Tth111I GACNNNGTC 1 cut(s) 937
Van91I CCANNNNNTGG 1 cut(s) 557
VneI GTGCAC 1 cut(s) 128
VpaK11BI GGWCC 2 cut(s) 1312, 2095
XagI CCTNNNNNAGG 1 cut(s) 1247
XapI RAATTY 3 cut(s) 10, 1777, 1796
XceI RCATGY 3 cut(s) 158, 716, 1193
XcmI CCANNNNNNNNNTGG 3 cut(s) 66, 400, 1819
XmiI GTMKAC 1 cut(s) 337
XmnI GAANNNNTTC 1 cut(s) 1905
XspI CTAG 6 cut(s) 462, 600, 1439, 1467, 1748, 1997
ZrmI AGTACT 1 cut(s) 1448
Zsp2I ATGCAT 2 cut(s) 399, 1435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.