pycom04g11700

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011147
Physical Location & Seq
Forward (+)
14551410 .. 14554563
3154 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g11700.3

Sequence Viewer

Length: 2466 bp
ATGGGAAAGAATTTGAGAGACAAAAACCCTAACAATTCATCAGGGAATGAGCATTCACCAGGTTGCGTTTGGGGATTACTTCATATGATGAAGTATCACCACTGGCATTATGTCAAGAAAAGACTTGTGCACAAGAGGCATCGCGGTAACAAACATGCTGTTGGAGTTGGGATTCCGGGAAGCTGTACTGGAAATGCCTGTATGCCAGAAGACACTGATGCGAAGAGTGACACATCTAACGGTGAAGAAAAGACGAGGCAGTTCATTCCAGCAGGCATACATTCCGTCAAGGCCCGAATAAAAGCACGGATTGCTGGAGAAATGTCAAAGAATAAGGGTAGGCATAACCGGGCTTCAAGCTGTCCTGTACGATCACAATTGAAACGAACAGAATCCATGCATCGTTTGGAACCACCACCTAACCTGTACCCGATTGCTGAAATGGTATTGAATGAGAATAGTCCTAGAATTGTTCACCACGGCAATGAAATATCCTGTGCTACAAGCAATCTGGCATCACTGTCCGCGACATCATATGAACGACCAATTAGTGGCAATAATGATTGTTGTGGAGATAGTTGCCCACTAATCACTAGAGATCGTACCGTGCATGACAAGGTTAATGAGAATAAAAAAGATCACACATTTATTGAAGAAAAGCTGGATGATGACAGAATGCAAGATATGTTCGAGAAGAAGTTGATACACGTAAAAGAACTCGATGCAGATGTTTCCCTACACCATCCAAAGGAGTATTTGGAGGCGTTGGATATAATCAATGTGAACAAGGAGTTACTAGTAAAGATTTTACATGATGCCGGCTCTCCTTTGGCTGCGCACTTCCATAATCAGCAAGCTTTCATTGCTAAAATGGGTTTAACTAAGTCAGAGTCATTCCCTATCAAGCTCAGGCACAAGCATGAATTCACAGGGTCTCCCTTAAAAGAGGAAAGCAAGTCGAAAAACAGTAGCAAAACAAAGAAGTTAGCAGGGTCGTCTTCTTTGAAATATGCTGGTGAACGCTCAATTCCATCGATCACTGAGCACAATGCAGATGGGATTCTAAAGCTAAATCAAACAATTGCCGATAGTTCTGCTTCTGGTTCTTCCGAAAATTTGAGGAACCAAGGTGAGAATCAAGTGGCAATTAAACGGTTCAAGGATCTGAGACAAAAAATAAAGCATGTAATCAAGGAGAGCAGACAAGAGAGACATATGATTGCCATGGATGCCATCCTTCACAAGGTTCCTCATGACCAAAAGCTTACTAAAGAAATGGAGCAAGAGATCATTAACCATTCGAAGGACCCTGCAATGAACAGAGAAGCTAAAGACAGTCCAGCAAGCAGTTATGATAGCGATCATTCGGTTTCTACCATCAAGAAAAAGCAACAGCACCACATGAGAAGAGCGTCATCACTCAATGCATCGCTAGATAAGTACTGTGAGTTGTACGAGACTAGTTGCAACAGAGAAGCCAAATGCCAAACAACGTCCGAGAGATTGAAGTTGAGAAATGAAAGGGTAGGCTCACCATTACGTTCTGTTCCAAAAACGCTGGGGAGGATTTTTTCGTTACCTGAAATTAAATCATATAATTATCAGAACGAGGAATCTTCTGATGTGTTTTCTTCTGAAGCTCCAATTAGGGCTGTTGTGGATGGAAATGCAAGCAGAAGAAGTAGCTTTGATGAACAAAATAGTGTACACCATAGTATAGGTTCAGAAAATCATATGCAGCTCGCTGTGCCTGTAGAAAGTGAGATTAAAAATTTGGCTGGCGGTGGTGAATTCAAGGCAGTTTCCAGAGATAAGATGGCATCAACATCAGTTGTAAACAGTGAGAAAAGTGCTCCGGTGGATTCAATTGTTAACGATATTGAAAGCTTGATAGAACCATTTCCAATCTCTTTGCCCGAGTTCAACGTTTCCCTCTCTGAAGGAGCAGAAATTGAAGTAAAACCTCGAGATGGAATAGAAAGCTTGGCCAAGCTAGAGCATGATTCTCTTATTGATACACCTAAACCGGTTCACATGGATACGGAGAAGATAGGAAGTCCAATGAAGCACTTGAGGTACGAAATGACTCATGTCCGCGTTGATGAAAGAGACATGGCTGAATTCAATTATGTGCGAGATGTGTTGGAGCAATCCGGATTCAGTGGAAATGAGTCCCTTGGGACATGGCATTCGGATGAGCAGCCTGTTGATCCTTTGATGTATCTAGACACTGAAGGCTGCTTGGTGCATGATCCTGATTGTTCAGGAAACGAAGAAGGCGGGAAATGTGATCACTATCTTTTGTTTGATCTGATCAATGAGGTGCTGATGGAGATATATCAGATCATACAACTACTGTCCGATGGCGTTGTCTTCCTTATGTCACATTCCTCTAATGCCGGCAGGGCACCATGTTTTGAAGGAAGTTTGGGCCCTTGTGAGTTGGAACTTGAGCTTGATACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3741 DUF4378

Protein Analysis

822

Amino Acids

91.93

Weight (kDa)

6.34

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02390 AT5G02390 AT5G02390
fragaria_vesca FvH4_6g13720
malus_domestica MD04G1128800.v1.1 MD12G1141400.v1.1
prunus_persica Prupe.6G254700_v2.0.a1
pyrus_communis pycom04g11700 pycom12g13630
rosa_chinensis RchiOBHm_Chr3g0466591
rosa_laevigata RLG00000024561
rosa_multiflora Rmu_sc0020778.1_g000002
rosa_roxburghii Rroxscaffold_6G00414320
rosa_rugosa Rorug03G0083000.1 Rorug03G0083100
rosa_samantha Rh3AG129900 Rh3BG151300 Rh3CG151000 Rh3DG151900
rosa_wichuraiana Rw3G012280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 837
AccB1I GGYRCC 1 cut(s) 2407
AccB7I CCANNNNNTGG 1 cut(s) 551
AccII CGCG 3 cut(s) 144, 527, 2097
AccIII TCCGGA 1 cut(s) 2153
AciI CCGC 5 cut(s) 144, 525, 1782, 2095, 2280
AclI AACGTT 1 cut(s) 1926
AclWI GGATC 3 cut(s) 1170, 2204, 2246
AcoI YGGCCR 1 cut(s) 1986
AcsI RAATTY 6 cut(s) 10, 923, 1114, 1771, 1790, 2120
AcuI CTGAAG 3 cut(s) 1656, 1959, 2253
AfaI GTAC 8 cut(s) 187, 369, 428, 604, 1442, 1454, 1707, 2078
AfiI CCNNNNNNNGG 5 cut(s) 551, 748, 1243, 1303, 1970
AflIII ACRYGT 1 cut(s) 706
AgeI ACCGGT 1 cut(s) 2026
AhlI ACTAGT 2 cut(s) 796, 1460
AjnI CCWGG 1 cut(s) 58
Alw21I GWGCWC 3 cut(s) 132, 1047, 1855
Alw26I GTCTC 6 cut(s) 12, 939, 1162, 1204, 1451, 2103
Alw44I GTGCAC 1 cut(s) 128
AlwI GGATC 3 cut(s) 1170, 2204, 2246
Ama87I CYCGRG 2 cut(s) 1916, 1965
Aor13HI TCCGGA 1 cut(s) 2153
AoxI GGCC 3 cut(s) 291, 1986, 2431
ApaI GGGCCC 1 cut(s) 2435
ApaLI GTGCAC 1 cut(s) 128
ApeKI GCWGC 4 cut(s) 833, 1738, 2200, 2238
ApoI RAATTY 6 cut(s) 10, 923, 1114, 1771, 1790, 2120
ArsI GACNNNNNNTTYG 2 cut(s) 2173, 2205
AsiGI ACCGGT 1 cut(s) 2026
Asp700I GAANNNNTTC 1 cut(s) 1899
AspLEI GCGC 1 cut(s) 838
AspS9I GGNCC 4 cut(s) 292, 1306, 2431, 2432
AsuC2I CCSGG 2 cut(s) 177, 350
AsuHPI GGTGA 8 cut(s) 48, 89, 254, 467, 1028, 1142, 1524, 1799
AsuII TTCGAA 1 cut(s) 1301
AvaI CYCGRG 2 cut(s) 1916, 1965
AvaII GGWCC 1 cut(s) 1306
BaeGI GKGCMC 3 cut(s) 132, 2410, 2435
BalI TGGCCA 1 cut(s) 1988
BanI GGYRCC 1 cut(s) 2407
BanII GRGCYC 1 cut(s) 2435
BbsI GAAGAC 3 cut(s) 216, 990, 2365
Bbv12I GWGCWC 3 cut(s) 132, 1047, 1855
BbvI GCAGC 4 cut(s) 820, 1750, 2212, 2225
BceAI ACGGC 1 cut(s) 496
BcgI CGANNNNNNTGC 2 cut(s) 1076, 1110
BciT130I CCWGG 1 cut(s) 60
BciVI GTATCC 1 cut(s) 2032
BclI TGATCA 2 cut(s) 2290, 2313
BcnI CCSGG 2 cut(s) 177, 350
BcoDI GTCTC 6 cut(s) 12, 939, 1162, 1204, 1451, 2103
BcuI ACTAGT 2 cut(s) 796, 1460
BfaI CTAG 7 cut(s) 465, 594, 797, 1433, 1461, 1994, 2225
BfmI CTRYAG 1 cut(s) 1752
BfuI GTATCC 1 cut(s) 2032
BglI GCCNNNNNGGC 1 cut(s) 2405
BisI GCNGC 4 cut(s) 834, 1739, 2201, 2239
BlsI GCNGC 4 cut(s) 835, 1740, 2202, 2240
BmcAI AGTACT 1 cut(s) 1442
Bme1390I CCNGG 3 cut(s) 60, 177, 350
Bme18I GGWCC 1 cut(s) 1306
BmeT110I CYCGRG 2 cut(s) 1916, 1965
BmgT120I GGNCC 4 cut(s) 292, 1306, 2431, 2432
BmiI GGNNCC 6 cut(s) 411, 1124, 1248, 1308, 2409, 2433
BmrFI CCNGG 3 cut(s) 60, 177, 350
BmsI GCATC 9 cut(s) 148, 208, 409, 524, 712, 805, 1219, 1436, 1829
BoxI GACNNNNGTC 1 cut(s) 2090
BpiI GAAGAC 3 cut(s) 216, 990, 2365
BpmI CTGGAG 1 cut(s) 336
Bpu10I CCTNAGC 1 cut(s) 908
Bpu14I TTCGAA 1 cut(s) 1301
BpuEI CTTGAG 1 cut(s) 2092
BpuMI CCSGG 2 cut(s) 177, 350
Bsa29I ATCGAT 1 cut(s) 1034
BsaAI YACGTR 1 cut(s) 709
BsaBI GATNNNNATC 2 cut(s) 1359, 2295
BsaI GGTCTC 1 cut(s) 939
BsaJI CCNNGG 4 cut(s) 478, 1126, 1224, 2176
BsaWI WCCGGW 3 cut(s) 1855, 2026, 2153
BsaXI ACNNNNNCTCC 2 cut(s) 919, 949
Bsc4I CCNNNNNNNGG 5 cut(s) 551, 748, 1243, 1303, 1970
Bse118I RCCGGY 3 cut(s) 818, 2026, 2399
Bse1I ACTGG 2 cut(s) 107, 193
Bse3DI GCAATG 3 cut(s) 490, 861, 1320
Bse8I GATNNNNATC 2 cut(s) 1359, 2295
BseAI TCCGGA 1 cut(s) 2153
BseBI CCWGG 1 cut(s) 60
BseCI ATCGAT 1 cut(s) 1034
BseDI CCNNGG 4 cut(s) 478, 1126, 1224, 2176
BseGI GGATG 6 cut(s) 670, 742, 1233, 1234, 1666, 2200
BseJI GATNNNNATC 2 cut(s) 1359, 2295
BseLI CCNNNNNNNGG 5 cut(s) 551, 748, 1243, 1303, 1970
BseMI GCAATG 3 cut(s) 490, 861, 1320
BseMII CTCAG 3 cut(s) 922, 1032, 1157
BseNI ACTGG 2 cut(s) 107, 193
BseSI GKGCMC 3 cut(s) 132, 2410, 2435
BseXI GCAGC 4 cut(s) 820, 1750, 2212, 2225
BseYI CCCAGC 1 cut(s) 1558
Bsh1236I CGCG 3 cut(s) 144, 527, 2097
BshFI GGCC 3 cut(s) 293, 1988, 2433
BshNI GGYRCC 1 cut(s) 2407
BshTI ACCGGT 1 cut(s) 2026
BshVI ATCGAT 1 cut(s) 1034
BsiHKAI GWGCWC 3 cut(s) 132, 1047, 1855
BsiHKCI CYCGRG 2 cut(s) 1916, 1965
BsiSI CCGG 7 cut(s) 176, 349, 819, 1856, 2027, 2154, 2400
BslFI GGGAC 2 cut(s) 2158, 2194
BslI CCNNNNNNNGG 5 cut(s) 551, 748, 1243, 1303, 1970
BsmAI GTCTC 6 cut(s) 12, 939, 1162, 1204, 1451, 2103
BsmFI GGGAC 2 cut(s) 2158, 2194
BsmI GAATGC 3 cut(s) 52, 681, 2188
BsnI GGCC 3 cut(s) 293, 1988, 2433
Bso31I GGTCTC 1 cut(s) 939
BsoBI CYCGRG 2 cut(s) 1916, 1965
Bsp119I TTCGAA 1 cut(s) 1301
Bsp120I GGGCCC 1 cut(s) 2431
Bsp1286I GDGCHC 5 cut(s) 132, 1047, 1855, 2410, 2435
Bsp13I TCCGGA 1 cut(s) 2153
Bsp1407I TGTACA 1 cut(s) 1705
Bsp19I CCATGG 1 cut(s) 1224
BspACI CCGC 5 cut(s) 144, 525, 1782, 2095, 2280
BspANI GGCC 3 cut(s) 293, 1988, 2433
BspCNI CTCAG 3 cut(s) 921, 1033, 1158
BspDI ATCGAT 1 cut(s) 1034
BspEI TCCGGA 1 cut(s) 2153
BspFNI CGCG 3 cut(s) 144, 527, 2097
BspHI TCATGA 1 cut(s) 1252
BspLI GGNNCC 6 cut(s) 411, 1124, 1248, 1308, 2409, 2433
BspPI GGATC 3 cut(s) 1170, 2204, 2246
BspQI GCTCTTC 1 cut(s) 1402
BspT104I TTCGAA 1 cut(s) 1301
BspT107I GGYRCC 1 cut(s) 2407
BspTNI GGTCTC 1 cut(s) 939
BsrDI GCAATG 3 cut(s) 490, 861, 1320
BsrFI RCCGGY 3 cut(s) 818, 2026, 2399
BsrGI TGTACA 1 cut(s) 1705
BsrI ACTGG 2 cut(s) 107, 193
BssAI RCCGGY 3 cut(s) 818, 2026, 2399
BssECI CCNNGG 4 cut(s) 478, 1126, 1224, 2176
BssT1I CCWWGG 3 cut(s) 1126, 1224, 2176
Bst2UI CCWGG 1 cut(s) 60
Bst4CI ACNGT 9 cut(s) 242, 522, 607, 968, 1155, 1337, 1445, 1841, 2358
Bst6I CTCTTC 2 cut(s) 218, 1402
BstAPI GCANNNNNTGC 1 cut(s) 311
BstAUI TGTACA 1 cut(s) 1705
BstBAI YACGTR 1 cut(s) 709
BstBI TTCGAA 1 cut(s) 1301
BstC8I GCNNGC 8 cut(s) 274, 820, 855, 1345, 1672, 1743, 1780, 2401
BstDEI CTNAG 4 cut(s) 882, 908, 1041, 1166
BstDSI CCRYGG 2 cut(s) 478, 1224
BstENI CCTNNNNNAGG 1 cut(s) 1241
BstF5I GGATG 6 cut(s) 670, 742, 1233, 1234, 1666, 2200
BstFNI CGCG 3 cut(s) 144, 527, 2097
BstHHI GCGC 1 cut(s) 838
BstMAI GTCTC 6 cut(s) 12, 939, 1162, 1204, 1451, 2103
BstMWI GCNNNNNNNGC 6 cut(s) 136, 311, 863, 1229, 1747, 2405
BstNI CCWGG 1 cut(s) 60
BstNSI RCATGY 2 cut(s) 158, 1187
BstPAI GACNNNNGTC 1 cut(s) 2090
BstSCI CCNGG 3 cut(s) 58, 175, 348
BstSFI CTRYAG 1 cut(s) 1752
BstSLI GKGCMC 3 cut(s) 132, 2410, 2435
BstUI CGCG 3 cut(s) 144, 527, 2097
BstV1I GCAGC 4 cut(s) 820, 1750, 2212, 2225
BstV2I GAAGAC 3 cut(s) 216, 990, 2365
BstX2I RGATCY 1 cut(s) 1162
BstYI RGATCY 1 cut(s) 1162
Bsu15I ATCGAT 1 cut(s) 1034
BsuI GTATCC 1 cut(s) 2032
BsuRI GGCC 3 cut(s) 293, 1988, 2433
BsuTUI ATCGAT 1 cut(s) 1034
BtgI CCRYGG 2 cut(s) 478, 1224
BtgZI GCGATG 2 cut(s) 125, 1413
BtsCI GGATG 6 cut(s) 670, 742, 1233, 1234, 1666, 2200
BtsIMutI CAGTG 7 cut(s) 100, 213, 518, 1038, 1846, 2167, 2229
Cac8I GCNNGC 8 cut(s) 274, 820, 855, 1345, 1672, 1743, 1780, 2401
CciI TCATGA 1 cut(s) 1252
CfoI GCGC 1 cut(s) 838
Cfr10I RCCGGY 3 cut(s) 818, 2026, 2399
Cfr13I GGNCC 4 cut(s) 292, 1306, 2431, 2432
ClaI ATCGAT 1 cut(s) 1034
CseI GACGC 1 cut(s) 1401
CsiI ACCWGGT 1 cut(s) 58
Csp6I GTAC 8 cut(s) 186, 368, 427, 603, 1441, 1453, 1706, 2077
CspAI ACCGGT 1 cut(s) 2026
CviQI GTAC 8 cut(s) 186, 368, 427, 603, 1441, 1453, 1706, 2077
DdeI CTNAG 4 cut(s) 882, 908, 1041, 1166
EaeI YGGCCR 1 cut(s) 1986
Eam1104I CTCTTC 2 cut(s) 218, 1402
EarI CTCTTC 2 cut(s) 218, 1402
Eco130I CCWWGG 3 cut(s) 1126, 1224, 2176
Eco24I GRGCYC 1 cut(s) 2435
Eco31I GGTCTC 1 cut(s) 939
Eco47I GGWCC 1 cut(s) 1306
Eco57I CTGAAG 3 cut(s) 1656, 1959, 2253
Eco88I CYCGRG 2 cut(s) 1916, 1965
EcoNI CCTNNNNNAGG 1 cut(s) 1241
EcoO109I RGGNCCY 2 cut(s) 1306, 2432
EcoRI GAATTC 3 cut(s) 923, 1790, 2120
EcoRII CCWGG 1 cut(s) 58
EcoT14I CCWWGG 3 cut(s) 1126, 1224, 2176
EcoT22I ATGCAT 2 cut(s) 402, 1429
EcoT38I GRGCYC 1 cut(s) 2435
ErhI CCWWGG 3 cut(s) 1126, 1224, 2176
FaqI GGGAC 2 cut(s) 2158, 2194
FauI CCCGC 1 cut(s) 2273
FauNDI CATATG 4 cut(s) 84, 535, 1215, 1734
FbaI TGATCA 2 cut(s) 2290, 2313
Fnu4HI GCNGC 4 cut(s) 834, 1739, 2201, 2239
FokI GGATG 6 cut(s) 677, 729, 1220, 1241, 1673, 2207
FriOI GRGCYC 1 cut(s) 2435
Fsp4HI GCNGC 4 cut(s) 834, 1739, 2201, 2239
FspBI CTAG 7 cut(s) 465, 594, 797, 1433, 1461, 1994, 2225
FspI TGCGCA 1 cut(s) 837
GlaI GCGC 1 cut(s) 837
GluI GCNGC 4 cut(s) 834, 1739, 2201, 2239
GsaI CCCAGC 1 cut(s) 1562
GsuI CTGGAG 1 cut(s) 336
HaeIII GGCC 3 cut(s) 293, 1988, 2433
HapII CCGG 7 cut(s) 176, 349, 819, 1856, 2027, 2154, 2400
HgaI GACGC 1 cut(s) 1401
HhaI GCGC 1 cut(s) 838
Hin6I GCGC 1 cut(s) 836
HinP1I GCGC 1 cut(s) 836
HincII GTYRAC 1 cut(s) 1873
HindII GTYRAC 1 cut(s) 1873
HindIII AAGCTT 4 cut(s) 855, 1262, 1885, 1981
HpaI GTTAAC 1 cut(s) 1873
HpaII CCGG 7 cut(s) 176, 349, 819, 1856, 2027, 2154, 2400
HphI GGTGA 8 cut(s) 48, 89, 254, 467, 1028, 1142, 1524, 1799
Hpy166II GTNNAC 9 cut(s) 130, 475, 784, 1019, 1706, 1708, 1837, 1873, 2032
Hpy8I GTNNAC 9 cut(s) 130, 475, 784, 1019, 1706, 1708, 1837, 1873, 2032
HpyAV CCTTC 6 cut(s) 1247, 1297, 1934, 2228, 2270, 2414
HpyCH4III ACNGT 9 cut(s) 242, 522, 607, 968, 1155, 1337, 1445, 1841, 2358
HpyCH4IV ACGT 4 cut(s) 708, 1493, 1540, 1926
HpyF10VI GCNNNNNNNGC 6 cut(s) 136, 311, 863, 1229, 1747, 2405
HpyF3I CTNAG 4 cut(s) 882, 908, 1041, 1166
HpySE526I ACGT 4 cut(s) 708, 1493, 1540, 1926
HspAI GCGC 1 cut(s) 836
Kpn2I TCCGGA 1 cut(s) 2153
KroI GCCGGC 2 cut(s) 818, 2399
KroNI GCCGGC 2 cut(s) 820, 2401
Ksp22I TGATCA 2 cut(s) 2290, 2313
KspAI GTTAAC 1 cut(s) 1873
LguI GCTCTTC 1 cut(s) 1402
LmnI GCTCC 5 cut(s) 1279, 1645, 1858, 1943, 2146
Lsp1109I GCAGC 4 cut(s) 820, 1750, 2212, 2225
LweI GCATC 9 cut(s) 148, 208, 409, 524, 712, 805, 1219, 1436, 1829
MabI ACCWGGT 1 cut(s) 58
MaeI CTAG 7 cut(s) 465, 594, 797, 1433, 1461, 1994, 2225
MaeII ACGT 4 cut(s) 708, 1493, 1540, 1926
MaeIII GTNAC 5 cut(s) 146, 227, 792, 1575, 2382
MfeI CAATTG 3 cut(s) 377, 1080, 1866
MflI RGATCY 1 cut(s) 1162
MhlI GDGCHC 5 cut(s) 132, 1047, 1855, 2410, 2435
MlsI TGGCCA 1 cut(s) 1988
MluNI TGGCCA 1 cut(s) 1988
MlyI GAGTC 3 cut(s) 899, 2080, 2180
MmeI TCCRAC 4 cut(s) 142, 747, 2124, 2424
Mox20I TGGCCA 1 cut(s) 1988
Mph1103I ATGCAT 2 cut(s) 402, 1429
MroI TCCGGA 1 cut(s) 2153
MroNI GCCGGC 2 cut(s) 818, 2399
MroXI GAANNNNTTC 1 cut(s) 1899
MscI TGGCCA 1 cut(s) 1988
MseI TTAA 8 cut(s) 621, 878, 941, 1149, 1293, 1587, 1767, 1872
MslI CAYNNNNRTG 3 cut(s) 483, 918, 2193
Msp20I TGGCCA 1 cut(s) 1988
MspI CCGG 7 cut(s) 176, 349, 819, 1856, 2027, 2154, 2400
MspR9I CCNGG 3 cut(s) 60, 177, 350
MunI CAATTG 3 cut(s) 377, 1080, 1866
Mva1269I GAATGC 3 cut(s) 52, 681, 2188
MvaI CCWGG 1 cut(s) 60
MvnI CGCG 3 cut(s) 144, 527, 2097
MwoI GCNNNNNNNGC 6 cut(s) 136, 311, 863, 1229, 1747, 2405
NaeI GCCGGC 2 cut(s) 820, 2401
NciI CCSGG 2 cut(s) 177, 350
NcoI CCATGG 1 cut(s) 1224
NdeI CATATG 4 cut(s) 84, 535, 1215, 1734
NgoMIV GCCGGC 2 cut(s) 818, 2399
NlaIV GGNNCC 6 cut(s) 411, 1124, 1248, 1308, 2409, 2433
NmuCI GTSAC 2 cut(s) 227, 2382
NsbI TGCGCA 1 cut(s) 837
NsiI ATGCAT 2 cut(s) 402, 1429
NspI RCATGY 2 cut(s) 158, 1187
NspV TTCGAA 1 cut(s) 1301
PaeR7I CTCGAG 1 cut(s) 1965
PagI TCATGA 1 cut(s) 1252
PciSI GCTCTTC 1 cut(s) 1402
PctI GAATGC 3 cut(s) 52, 681, 2188
PdiI GCCGGC 2 cut(s) 820, 2401
PdmI GAANNNNTTC 1 cut(s) 1899
PfeI GAWTC 8 cut(s) 172, 392, 1060, 1135, 1613, 1862, 2003, 2157
PflMI CCANNNNNTGG 1 cut(s) 551
PfoI TCCNGGA 1 cut(s) 175
PinAI ACCGGT 1 cut(s) 2026
PkrI GCNGC 4 cut(s) 835, 1740, 2202, 2240
PleI GAGTC 3 cut(s) 898, 2080, 2179
PpsI GAGTC 3 cut(s) 898, 2080, 2179
Ppu21I YACGTR 1 cut(s) 709
PpuMI RGGWCCY 1 cut(s) 1306
PshAI GACNNNNGTC 1 cut(s) 2090
Psp1406I AACGTT 1 cut(s) 1926
Psp5II RGGWCCY 1 cut(s) 1306
Psp6I CCWGG 1 cut(s) 58
PspFI CCCAGC 1 cut(s) 1558
PspGI CCWGG 1 cut(s) 58
PspN4I GGNNCC 6 cut(s) 411, 1124, 1248, 1308, 2409, 2433
PspOMI GGGCCC 1 cut(s) 2431
PspPI GGNCC 4 cut(s) 292, 1306, 2431, 2432
PspPPI RGGWCCY 1 cut(s) 1306
PsuI RGATCY 1 cut(s) 1162
RsaI GTAC 8 cut(s) 187, 369, 428, 604, 1442, 1454, 1707, 2078
RsaNI GTAC 8 cut(s) 186, 368, 427, 603, 1441, 1453, 1706, 2077
RseI CAYNNNNRTG 3 cut(s) 483, 918, 2193
SapI GCTCTTC 1 cut(s) 1402
SaqAI TTAA 8 cut(s) 621, 878, 941, 1149, 1293, 1587, 1767, 1872
SatI GCNGC 4 cut(s) 834, 1739, 2201, 2239
Sau96I GGNCC 4 cut(s) 292, 1306, 2431, 2432
ScaI AGTACT 1 cut(s) 1442
SchI GAGTC 3 cut(s) 899, 2080, 2180
ScrFI CCNGG 3 cut(s) 60, 177, 350
SduI GDGCHC 5 cut(s) 132, 1047, 1855, 2410, 2435
SexAI ACCWGGT 1 cut(s) 58
SfaNI GCATC 9 cut(s) 148, 208, 409, 524, 712, 805, 1219, 1436, 1829
SfcI CTRYAG 1 cut(s) 1752
Sfr274I CTCGAG 1 cut(s) 1965
SfuI TTCGAA 1 cut(s) 1301
SinI GGWCC 1 cut(s) 1306
SlaI CTCGAG 1 cut(s) 1965
SmiMI CAYNNNNRTG 3 cut(s) 483, 918, 2193
SmlI CTYRAG 3 cut(s) 1965, 2071, 2450
SmoI CTYRAG 3 cut(s) 1965, 2071, 2450
SpeI ACTAGT 2 cut(s) 796, 1460
SsiI CCGC 5 cut(s) 144, 525, 1782, 2095, 2280
SspMI CTAG 7 cut(s) 465, 594, 797, 1433, 1461, 1994, 2225
StyD4I CCNGG 3 cut(s) 58, 175, 348
StyI CCWWGG 3 cut(s) 1126, 1224, 2176
TaaI ACNGT 9 cut(s) 242, 522, 607, 968, 1155, 1337, 1445, 1841, 2358
TaiI ACGT 4 cut(s) 711, 1496, 1543, 1929
TaqI TCGA 6 cut(s) 690, 720, 959, 1034, 1301, 1966
TatI WGTACW 3 cut(s) 185, 1440, 1705
TfiI GAWTC 8 cut(s) 172, 392, 1060, 1135, 1613, 1862, 2003, 2157
Tru1I TTAA 8 cut(s) 621, 878, 941, 1149, 1293, 1587, 1767, 1872
Tru9I TTAA 8 cut(s) 621, 878, 941, 1149, 1293, 1587, 1767, 1872
TscAI CASTG 7 cut(s) 107, 220, 525, 1045, 1846, 2167, 2236
TseFI GTSAC 2 cut(s) 227, 2382
TseI GCWGC 4 cut(s) 833, 1738, 2200, 2238
Tsp45I GTSAC 2 cut(s) 227, 2382
TspGWI ACGGA 3 cut(s) 274, 322, 2057
TspRI CASTG 7 cut(s) 107, 220, 525, 1045, 1846, 2167, 2236
Van91I CCANNNNNTGG 1 cut(s) 551
VneI GTGCAC 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 1306
XagI CCTNNNNNAGG 1 cut(s) 1241
XapI RAATTY 6 cut(s) 10, 923, 1114, 1771, 1790, 2120
XbaI TCTAGA 1 cut(s) 2224
XceI RCATGY 2 cut(s) 158, 1187
XcmI CCANNNNNNNNNTGG 3 cut(s) 66, 403, 1813
XhoI CTCGAG 1 cut(s) 1965
XmnI GAANNNNTTC 1 cut(s) 1899
XspI CTAG 7 cut(s) 465, 594, 797, 1433, 1461, 1994, 2225
ZrmI AGTACT 1 cut(s) 1442
Zsp2I ATGCAT 2 cut(s) 402, 1429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.