Rh3AG129900

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0011147
Physical Location & Seq
Reverse (-)
11216213 .. 11220790
4578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG129900.1

Sequence Viewer

Length: 2196 bp
ATGGGAAGGAACTTGCGAGAAAAACAGCCTAGGGATGCAACAGGGAATGAGCGTCCAGGGACTCCAGGGTGTGTATGGGGCATGCTACATGTCATGAAGTATCACCACTGGCATTATGTCAAGAAAAGACTAATGTACAAGAAGCATGGCCATAATAAAAATGGTGCAGGAGTTGGAAATCATGGGAATTGTGGAAATGCATGTAGTCTTCAGAATATACAAGACAATGATGCTAAGATGGACAAAACTACTCCTGCCGAAGAAATGATAAATCCGTTGAGTCCAACATCCAAGGCTTCTGTTAAGGCCCGAATAAAGGCACGAATTGCTGAAGAAATGGCTAAGAAGAGGGGCAGATATAACCGGACCTCAAGCTGTCCTGTACGATCACAATTGACACGAACAGAATCGATACATCATTTCGAACCACCTTCAAATATGGATCCAATTGCTGAAATGGTATTGAATGAGGCAAGTCCTCGACCTGTTGCTCACAAAAACAAGGAAAGCTCTTCTTCTGAAGAACCTAGTACTAGTGTCGACAACAATTGTGCAGAGAGCAGTCCAATGAGTGTTACTGGAGATCCCACAGTGGATCATGTCCCGGTTAATGAGAACCAAAAGGATGAGCCACTTAGTCAAGAACAGCTAGACAATGACACAGTACAAGATATGTTACAGAAGAAGTTGATACATGTTAAAGAACTTGACGCAGATTCTTCGGTCCATCAAAAGGAGTATCTAGAGGCTTTGGATATAATTAATGTAAACAAAGAGTTACTAGTGAAAATTTTACATGATCCGGGTTCTCCTCTAGTTCAGCACTTCCAGAATCAACAAGCAGTCAGCGCAAAAACGAGTTTAACCAAGGCAGAGTCATTTCCTATGCCCGGTTCATCAGGCAAAGGAGGTTCTGAACCTACTAAGCTCAAACCCAAGCATGAGCCAAAAGATGGTAGTCCAGCTCGAAAACCTACATGGTCTACTTCATTGAAATATGCTGGGGAACGGCCAATGCCATCGATTACTGATCAGAATATGGAGAGTTCATCTTCCCCTTCTCAACCCAGTTTGATGAGTCAAGGTGAGAATCCGGTGGCAATTAAGCGTTTCAAGGATCTTAGACAAAAAATAAAGCACGTGATTAAGGAGAGTAAACAAGAGAGACATGTGATTTCCATGGAGGCCATCCTTCACAAGATTCCTCATGGACAAAAGCTCACTAAAGAACTAGAGCAGGAGATTGTTAGCCATTCGAAGGACCCTGCATTGCATAGAGAAGGTAAAGATAGTCCAAGTAGTTATGGAAGTGATCATTCAGTTTCTCCCCTAAAGAAAAAAATGCCCAACATGAGAAGAACATCATCTTTCGATGCATCACTTGATAGGTACCGCAGGTTGTATGAGATGACTAGTTTCAAGAAAGAGGACAAATGCCAGCCATCATATGAGAGATTGAAAGTTAGAGTAGAAGATTCAGGCTCACCATTCCAGAGCTCACCTTTCCGGAAAGCTCCCAAAAGTCTGGGAAGAATTTACTCTTTACCTGATATGGAATCATACTATCAGAATGAGGAATCTCCAGATGGTTTTCCTCCTCCATTGCTATTTAAGACCGGTGTGGATGGAAATGCAAGCACAAAAAGCAGATTTAGTGAACAAAAGAGGGAGCTTTCTATAGGTTCAGAAAATGAGTTGCAGTTAGATTTTCCTATAGAAAGTGAAGTCCAAGCAAACTTGGATGATGCTACTGAAACTAATGCAGTTACTAGAGATAAGGTGGAATCTACATCAGTTGTGCACAATGAGACAAGTTCTAAGGTGTCAAATGATGAATTTGAAAAATTGGCAACAGGGGACAGTATTTCTCACAAAGAACAAGATACTAAAACTAATGCAGTTACTAGAGATAAGGTGGAATCTACATCAGTTGCGCATGATGAGACAAGTTCTAAGGTGTCAAAAGATGAATTTGAAAAAATAACAACTGGGGACAGTGTTTCTCATACAGAACAAGATACTGAGCCTCTTTTAGAAGCCATTACTAGTGTTTCAGATCCCTTACCCACGTACGTGCCTAACATCAATGCTCTACAAGATATTTCCAGCCTGAGAAAATGTTCCCAGTCTGAAGGTTTGTTTTTCCAGACGTCATCAGTAGATGTTACCGTTATATTGTCTCAAACCAACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3741 DUF4378

Protein Analysis

731

Amino Acids

81.48

Weight (kDa)

6.48

Isoelectric Point (pI)

55.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3741 PF12552 228 - 270 9.1e-12 Protein of unknown function (DUF3741)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02390 AT5G02390 AT5G02390
fragaria_vesca FvH4_6g13720
malus_domestica MD04G1128800.v1.1 MD12G1141400.v1.1
prunus_persica Prupe.6G254700_v2.0.a1
pyrus_communis pycom04g11700 pycom12g13630
rosa_chinensis RchiOBHm_Chr3g0466591
rosa_laevigata RLG00000024561
rosa_multiflora Rmu_sc0020778.1_g000002
rosa_roxburghii Rroxscaffold_6G00414320
rosa_rugosa Rorug03G0083000.1 Rorug03G0083100
rosa_samantha Rh3AG129900 Rh3BG151300 Rh3CG151000 Rh3DG151900
rosa_wichuraiana Rw3G012280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2153
Acc16I TGCGCA 1 cut(s) 1935
Acc36I ACCTGC 1 cut(s) 1386
Acc65I GGTACC 1 cut(s) 1389
AccB1I GGYRCC 1 cut(s) 1389
AccB7I CCANNNNNTGG 1 cut(s) 953
AccI GTMKAC 2 cut(s) 540, 983
AccIII TCCGGA 1 cut(s) 1506
AciI CCGC 1 cut(s) 1393
AclWI GGATC 7 cut(s) 437, 450, 578, 603, 794, 1125, 2051
AcoI YGGCCR 2 cut(s) 148, 1010
AcsI RAATTY 4 cut(s) 789, 1533, 1835, 1970
AcuI CTGAAG 4 cut(s) 194, 351, 540, 2151
AcvI CACGTG 1 cut(s) 1141
AcyI GRCGYC 1 cut(s) 2150
AfaI GTAC 6 cut(s) 137, 384, 532, 666, 1391, 2072
AfiI CCNNNNNNNGG 5 cut(s) 316, 733, 890, 953, 1258
AflIII ACRYGT 4 cut(s) 88, 694, 1168, 2190
AgeI ACCGGT 1 cut(s) 1616
AgsI TTSAA 8 cut(s) 435, 466, 994, 1114, 1420, 1459, 1841, 1976
AhlI ACTAGT 4 cut(s) 533, 781, 1412, 2045
AjnI CCWGG 2 cut(s) 55, 64
AleI CACNNNNGTG 1 cut(s) 2072
AluBI AGCT 9 cut(s) 375, 510, 649, 928, 965, 1219, 1497, 1514, 1672
AluI AGCT 9 cut(s) 375, 510, 649, 928, 965, 1219, 1497, 1514, 1672
Alw21I GWGCWC 2 cut(s) 1499, 1803
Alw26I GTCTC 4 cut(s) 1159, 1802, 1937, 2184
Alw44I GTGCAC 1 cut(s) 1799
AlwI GGATC 7 cut(s) 437, 450, 578, 603, 794, 1125, 2051
Aor13HI TCCGGA 1 cut(s) 1506
AoxI GGCC 4 cut(s) 148, 306, 1010, 1185
ApaLI GTGCAC 1 cut(s) 1799
ApoI RAATTY 4 cut(s) 789, 1533, 1835, 1970
AseI ATTAAT 1 cut(s) 762
AsiGI ACCGGT 1 cut(s) 1616
Asp700I GAANNNNTTC 1 cut(s) 2119
Asp718I GGTACC 1 cut(s) 1389
AspA2I CCTAGG 1 cut(s) 29
AspLEI GCGC 2 cut(s) 851, 1936
AspS9I GGNCC 4 cut(s) 307, 366, 724, 1261
AsuC2I CCSGG 3 cut(s) 605, 804, 891
AsuHPI GGTGA 4 cut(s) 95, 1097, 1476, 1491
AsuII TTCGAA 2 cut(s) 423, 1256
AvaII GGWCC 3 cut(s) 366, 724, 1261
AvrII CCTAGG 1 cut(s) 29
BaeGI GKGCMC 1 cut(s) 1803
BalI TGGCCA 1 cut(s) 150
BamHI GGATCC 1 cut(s) 442
BanI GGYRCC 1 cut(s) 1389
BanII GRGCYC 1 cut(s) 1499
BbrPI CACGTG 1 cut(s) 1141
BbsI GAAGAC 1 cut(s) 200
Bbv12I GWGCWC 2 cut(s) 1499, 1803
BccI CCATC 8 cut(s) 232, 735, 947, 1027, 1196, 1450, 1580, 1619
BceAI ACGGC 1 cut(s) 1025
BcgI CGANNNNNNTGC 2 cut(s) 702, 736
BciT130I CCWGG 2 cut(s) 57, 66
BclI TGATCA 2 cut(s) 1030, 1312
BcnI CCSGG 3 cut(s) 605, 804, 891
BcoDI GTCTC 4 cut(s) 1159, 1802, 1937, 2184
BcuI ACTAGT 4 cut(s) 533, 781, 1412, 2045
BfmI CTRYAG 2 cut(s) 1677, 1713
BfuAI ACCTGC 1 cut(s) 1386
BlnI CCTAGG 1 cut(s) 29
BmcAI AGTACT 1 cut(s) 532
Bme1390I CCNGG 5 cut(s) 57, 66, 605, 804, 891
Bme18I GGWCC 3 cut(s) 366, 724, 1261
BmgT120I GGNCC 4 cut(s) 307, 366, 724, 1261
BmiI GGNNCC 3 cut(s) 444, 1263, 1391
BmrFI CCNGG 5 cut(s) 57, 66, 605, 804, 891
BmrI ACTGGG 3 cut(s) 1062, 1998, 2119
BmsI GCATC 5 cut(s) 25, 220, 1363, 1385, 1735
BmuI ACTGGG 3 cut(s) 1062, 1998, 2119
BpiI GAAGAC 1 cut(s) 200
BpmI CTGGAG 3 cut(s) 48, 600, 1566
Bpu14I TTCGAA 2 cut(s) 423, 1256
BpuEI CTTGAG 1 cut(s) 355
BpuMI CCSGG 3 cut(s) 605, 804, 891
Bsa29I ATCGAT 2 cut(s) 410, 1022
BsaAI YACGTR 3 cut(s) 1141, 2070, 2074
BsaHI GRCGYC 1 cut(s) 2150
BsaJI CCNNGG 6 cut(s) 29, 56, 65, 291, 867, 1179
BsaWI WCCGGW 4 cut(s) 363, 1093, 1506, 1616
Bsc4I CCNNNNNNNGG 5 cut(s) 316, 733, 890, 953, 1258
Bse118I RCCGGY 1 cut(s) 1616
Bse1I ACTGG 5 cut(s) 113, 583, 1068, 1993, 2125
Bse3DI GCAATG 2 cut(s) 1268, 1601
BseAI TCCGGA 1 cut(s) 1506
BseBI CCWGG 2 cut(s) 57, 66
BseCI ATCGAT 2 cut(s) 410, 1022
BseDI CCNNGG 6 cut(s) 29, 56, 65, 291, 867, 1179
BseGI GGATG 6 cut(s) 40, 287, 631, 1188, 1630, 1747
BseLI CCNNNNNNNGG 5 cut(s) 316, 733, 890, 953, 1258
BseMI GCAATG 2 cut(s) 1268, 1601
BseMII CTCAG 2 cut(s) 2013, 2102
BseNI ACTGG 5 cut(s) 113, 583, 1068, 1993, 2125
BseRI GAGGAG 2 cut(s) 801, 1587
BseSI GKGCMC 1 cut(s) 1803
BseYI CCCAGC 1 cut(s) 1001
BsgI GTGCAG 2 cut(s) 186, 573
BshFI GGCC 4 cut(s) 150, 308, 1012, 1187
BshNI GGYRCC 1 cut(s) 1389
BshTI ACCGGT 1 cut(s) 1616
BshVI ATCGAT 2 cut(s) 410, 1022
BsiHKAI GWGCWC 2 cut(s) 1499, 1803
BsiSI CCGG 7 cut(s) 364, 605, 803, 891, 1094, 1507, 1617
BsiWI CGTACG 1 cut(s) 2070
BslFI GGGAC 4 cut(s) 73, 587, 1871, 2006
BslI CCNNNNNNNGG 5 cut(s) 316, 733, 890, 953, 1258
BsmAI GTCTC 4 cut(s) 1159, 1802, 1937, 2184
BsmFI GGGAC 4 cut(s) 73, 587, 1871, 2006
BsnI GGCC 4 cut(s) 150, 308, 1012, 1187
Bsp119I TTCGAA 2 cut(s) 423, 1256
Bsp1286I GDGCHC 2 cut(s) 1499, 1803
Bsp13I TCCGGA 1 cut(s) 1506
Bsp1407I TGTACA 1 cut(s) 135
Bsp143I GATC 9 cut(s) 386, 442, 583, 595, 799, 1030, 1117, 1312, 2056
Bsp19I CCATGG 1 cut(s) 1179
BspACI CCGC 1 cut(s) 1393
BspANI GGCC 4 cut(s) 150, 308, 1012, 1187
BspCNI CTCAG 2 cut(s) 2014, 2103
BspDI ATCGAT 2 cut(s) 410, 1022
BspEI TCCGGA 1 cut(s) 1506
BspHI TCATGA 1 cut(s) 93
BspLI GGNNCC 3 cut(s) 444, 1263, 1391
BspMI ACCTGC 1 cut(s) 1386
BspPI GGATC 7 cut(s) 437, 450, 578, 603, 794, 1125, 2051
BspQI GCTCTTC 1 cut(s) 517
BspT104I TTCGAA 2 cut(s) 423, 1256
BspT107I GGYRCC 1 cut(s) 1389
BsrDI GCAATG 2 cut(s) 1268, 1601
BsrFI RCCGGY 1 cut(s) 1616
BsrGI TGTACA 1 cut(s) 135
BsrI ACTGG 5 cut(s) 113, 583, 1068, 1993, 2125
BssAI RCCGGY 1 cut(s) 1616
BssECI CCNNGG 6 cut(s) 29, 56, 65, 291, 867, 1179
BssMI GATC 9 cut(s) 386, 442, 583, 595, 799, 1030, 1117, 1312, 2056
BssNI GRCGYC 1 cut(s) 2150
BssT1I CCWWGG 4 cut(s) 29, 291, 867, 1179
Bst2UI CCWGG 2 cut(s) 57, 66
Bst4CI ACNGT 5 cut(s) 592, 664, 1862, 1997, 2170
Bst6I CTCTTC 2 cut(s) 341, 517
BstACI GRCGYC 1 cut(s) 2150
BstAPI GCANNNNNTGC 1 cut(s) 326
BstAUI TGTACA 1 cut(s) 135
BstBAI YACGTR 3 cut(s) 1141, 2070, 2074
BstBI TTCGAA 2 cut(s) 423, 1256
BstC8I GCNNGC 3 cut(s) 83, 1439, 1636
BstDEI CTNAG 9 cut(s) 234, 342, 635, 924, 1121, 1818, 1953, 2022, 2111
BstDSI CCRYGG 1 cut(s) 1179
BstF5I GGATG 6 cut(s) 40, 287, 631, 1188, 1630, 1747
BstHHI GCGC 2 cut(s) 851, 1936
BstKTI GATC 9 cut(s) 389, 445, 586, 598, 802, 1033, 1120, 1315, 2059
BstMAI GTCTC 4 cut(s) 1159, 1802, 1937, 2184
BstMBI GATC 9 cut(s) 386, 442, 583, 595, 799, 1030, 1117, 1312, 2056
BstMWI GCNNNNNNNGC 3 cut(s) 326, 848, 1644
BstNI CCWGG 2 cut(s) 57, 66
BstNSI RCATGY 6 cut(s) 85, 92, 204, 698, 1172, 2194
BstSCI CCNGG 5 cut(s) 55, 64, 603, 802, 889
BstSFI CTRYAG 2 cut(s) 1677, 1713
BstSLI GKGCMC 1 cut(s) 1803
BstV2I GAAGAC 1 cut(s) 200
BstX2I RGATCY 4 cut(s) 442, 583, 1117, 2056
BstXI CCANNNNNNTGG 1 cut(s) 1525
BstYI RGATCY 4 cut(s) 442, 583, 1117, 2056
Bsu15I ATCGAT 2 cut(s) 410, 1022
BsuRI GGCC 4 cut(s) 150, 308, 1012, 1187
BsuTUI ATCGAT 2 cut(s) 410, 1022
BtgI CCRYGG 1 cut(s) 1179
BtsCI GGATG 6 cut(s) 40, 287, 631, 1188, 1630, 1747
BtsIMutI CAGTG 3 cut(s) 106, 597, 2002
BveI ACCTGC 1 cut(s) 1386
Cac8I GCNNGC 3 cut(s) 83, 1439, 1636
CciI TCATGA 1 cut(s) 93
CfoI GCGC 2 cut(s) 851, 1936
Cfr10I RCCGGY 1 cut(s) 1616
Cfr13I GGNCC 4 cut(s) 307, 366, 724, 1261
ClaI ATCGAT 2 cut(s) 410, 1022
CseI GACGC 2 cut(s) 41, 719
Csp6I GTAC 6 cut(s) 136, 383, 531, 665, 1390, 2071
CspAI ACCGGT 1 cut(s) 1616
CviQI GTAC 6 cut(s) 136, 383, 531, 665, 1390, 2071
DdeI CTNAG 9 cut(s) 234, 342, 635, 924, 1121, 1818, 1953, 2022, 2111
DpnI GATC 9 cut(s) 388, 444, 585, 597, 801, 1032, 1119, 1314, 2058
DpnII GATC 9 cut(s) 386, 442, 583, 595, 799, 1030, 1117, 1312, 2056
EaeI YGGCCR 2 cut(s) 148, 1010
Eam1104I CTCTTC 2 cut(s) 341, 517
EarI CTCTTC 2 cut(s) 341, 517
Ecl136II GAGCTC 1 cut(s) 1497
Eco130I CCWWGG 4 cut(s) 29, 291, 867, 1179
Eco24I GRGCYC 1 cut(s) 1499
Eco47I GGWCC 3 cut(s) 366, 724, 1261
Eco53kI GAGCTC 1 cut(s) 1497
Eco57I CTGAAG 4 cut(s) 194, 351, 540, 2151
Eco72I CACGTG 1 cut(s) 1141
EcoICRI GAGCTC 1 cut(s) 1497
EcoO109I RGGNCCY 1 cut(s) 1261
EcoRII CCWGG 2 cut(s) 55, 64
EcoT14I CCWWGG 4 cut(s) 29, 291, 867, 1179
EcoT22I ATGCAT 2 cut(s) 202, 1378
EcoT38I GRGCYC 1 cut(s) 1499
ErhI CCWWGG 4 cut(s) 29, 291, 867, 1179
FalI AAGNNNNNCTT 2 cut(s) 499, 531
FaqI GGGAC 4 cut(s) 73, 587, 1871, 2006
FauNDI CATATG 1 cut(s) 1447
FbaI TGATCA 2 cut(s) 1030, 1312
FblI GTMKAC 2 cut(s) 540, 983
FokI GGATG 6 cut(s) 47, 274, 638, 1175, 1637, 1754
FriOI GRGCYC 1 cut(s) 1499
FspI TGCGCA 1 cut(s) 1935
GlaI GCGC 2 cut(s) 850, 1935
GsaI CCCAGC 1 cut(s) 1005
GsuI CTGGAG 3 cut(s) 48, 600, 1566
HaeIII GGCC 4 cut(s) 150, 308, 1012, 1187
HapII CCGG 7 cut(s) 364, 605, 803, 891, 1094, 1507, 1617
HgaI GACGC 2 cut(s) 41, 719
HhaI GCGC 2 cut(s) 851, 1936
Hin1I GRCGYC 1 cut(s) 2150
Hin6I GCGC 2 cut(s) 849, 1934
HinP1I GCGC 2 cut(s) 849, 1934
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HpaII CCGG 7 cut(s) 364, 605, 803, 891, 1094, 1507, 1617
HphI GGTGA 4 cut(s) 95, 1097, 1476, 1491
Hpy166II GTNNAC 6 cut(s) 541, 769, 984, 1157, 1658, 1801
Hpy188I TCNGA 8 cut(s) 213, 520, 916, 1035, 1569, 1687, 2056, 2131
Hpy8I GTNNAC 6 cut(s) 541, 769, 984, 1157, 1658, 1801
HpyAV CCTTC 6 cut(s) 441, 1068, 1202, 1252, 1274, 2126
HpyCH4III ACNGT 5 cut(s) 592, 664, 1862, 1997, 2170
HpyCH4IV ACGT 4 cut(s) 1140, 2069, 2073, 2150
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 848, 1644
HpyF3I CTNAG 9 cut(s) 234, 342, 635, 924, 1121, 1818, 1953, 2022, 2111
HpySE526I ACGT 4 cut(s) 1140, 2069, 2073, 2150
Hsp92I GRCGYC 1 cut(s) 2150
HspAI GCGC 2 cut(s) 849, 1934
Kpn2I TCCGGA 1 cut(s) 1506
KpnI GGTACC 1 cut(s) 1393
Ksp22I TGATCA 2 cut(s) 1030, 1312
Kzo9I GATC 9 cut(s) 386, 442, 583, 595, 799, 1030, 1117, 1312, 2056
LguI GCTCTTC 1 cut(s) 517
LmnI GCTCC 2 cut(s) 1519, 1669
LweI GCATC 5 cut(s) 25, 220, 1363, 1385, 1735
MaeII ACGT 4 cut(s) 1140, 2069, 2073, 2150
MaeIII GTNAC 6 cut(s) 574, 675, 777, 1765, 1900, 2164
MalI GATC 9 cut(s) 388, 444, 585, 597, 801, 1032, 1119, 1314, 2058
MboI GATC 9 cut(s) 386, 442, 583, 595, 799, 1030, 1117, 1312, 2056
MfeI CAATTG 3 cut(s) 392, 447, 547
MflI RGATCY 4 cut(s) 442, 583, 1117, 2056
MhlI GDGCHC 2 cut(s) 1499, 1803
MlsI TGGCCA 1 cut(s) 150
MluNI TGGCCA 1 cut(s) 150
MlyI GAGTC 4 cut(s) 55, 289, 884, 1087
MmeI TCCRAC 2 cut(s) 154, 308
Mox20I TGGCCA 1 cut(s) 150
Mph1103I ATGCAT 2 cut(s) 202, 1378
MroI TCCGGA 1 cut(s) 1506
MroXI GAANNNNTTC 1 cut(s) 2119
MscI TGGCCA 1 cut(s) 150
MseI TTAA 8 cut(s) 303, 609, 699, 762, 863, 1104, 1146, 1611
MslI CAYNNNNRTG 1 cut(s) 2072
Msp20I TGGCCA 1 cut(s) 150
MspI CCGG 7 cut(s) 364, 605, 803, 891, 1094, 1507, 1617
MspR9I CCNGG 5 cut(s) 57, 66, 605, 804, 891
MunI CAATTG 3 cut(s) 392, 447, 547
MvaI CCWGG 2 cut(s) 57, 66
MwoI GCNNNNNNNGC 3 cut(s) 326, 848, 1644
NciI CCSGG 3 cut(s) 605, 804, 891
NcoI CCATGG 1 cut(s) 1179
NdeI CATATG 1 cut(s) 1447
NdeII GATC 9 cut(s) 386, 442, 583, 595, 799, 1030, 1117, 1312, 2056
NlaIV GGNNCC 3 cut(s) 444, 1263, 1391
NsbI TGCGCA 1 cut(s) 1935
NsiI ATGCAT 2 cut(s) 202, 1378
NspI RCATGY 6 cut(s) 85, 92, 204, 698, 1172, 2194
NspV TTCGAA 2 cut(s) 423, 1256
OliI CACNNNNGTG 1 cut(s) 2072
PaeI GCATGC 1 cut(s) 85
PagI TCATGA 1 cut(s) 93
PciI ACATGT 4 cut(s) 88, 694, 1168, 2190
PciSI GCTCTTC 1 cut(s) 517
PdmI GAANNNNTTC 1 cut(s) 2119
Pfl23II CGTACG 1 cut(s) 2070
PflMI CCANNNNNTGG 1 cut(s) 953
PinAI ACCGGT 1 cut(s) 1616
PleI GAGTC 4 cut(s) 55, 288, 883, 1086
PmaCI CACGTG 1 cut(s) 1141
PmlI CACGTG 1 cut(s) 1141
PpsI GAGTC 4 cut(s) 55, 288, 883, 1086
Ppu21I YACGTR 3 cut(s) 1141, 2070, 2074
PpuMI RGGWCCY 1 cut(s) 1261
PscI ACATGT 4 cut(s) 88, 694, 1168, 2190
PshBI ATTAAT 1 cut(s) 762
Psp124BI GAGCTC 1 cut(s) 1499
Psp5II RGGWCCY 1 cut(s) 1261
Psp6I CCWGG 2 cut(s) 55, 64
PspCI CACGTG 1 cut(s) 1141
PspFI CCCAGC 1 cut(s) 1001
PspGI CCWGG 2 cut(s) 55, 64
PspLI CGTACG 1 cut(s) 2070
PspN4I GGNNCC 3 cut(s) 444, 1263, 1391
PspPI GGNCC 4 cut(s) 307, 366, 724, 1261
PspPPI RGGWCCY 1 cut(s) 1261
PsuI RGATCY 4 cut(s) 442, 583, 1117, 2056
RsaI GTAC 6 cut(s) 137, 384, 532, 666, 1391, 2072
RsaNI GTAC 6 cut(s) 136, 383, 531, 665, 1390, 2071
RseI CAYNNNNRTG 1 cut(s) 2072
SacI GAGCTC 1 cut(s) 1499
SalI GTCGAC 1 cut(s) 539
SapI GCTCTTC 1 cut(s) 517
SaqAI TTAA 8 cut(s) 303, 609, 699, 762, 863, 1104, 1146, 1611
Sau3AI GATC 9 cut(s) 386, 442, 583, 595, 799, 1030, 1117, 1312, 2056
Sau96I GGNCC 4 cut(s) 307, 366, 724, 1261
ScaI AGTACT 1 cut(s) 532
SchI GAGTC 4 cut(s) 55, 289, 884, 1087
ScrFI CCNGG 5 cut(s) 57, 66, 605, 804, 891
SduI GDGCHC 2 cut(s) 1499, 1803
SfaNI GCATC 5 cut(s) 25, 220, 1363, 1385, 1735
SfcI CTRYAG 2 cut(s) 1677, 1713
SfuI TTCGAA 2 cut(s) 423, 1256
SinI GGWCC 3 cut(s) 366, 724, 1261
SmiMI CAYNNNNRTG 1 cut(s) 2072
SmlI CTYRAG 1 cut(s) 370
SmoI CTYRAG 1 cut(s) 370
SpeI ACTAGT 4 cut(s) 533, 781, 1412, 2045
SphI GCATGC 1 cut(s) 85
SsiI CCGC 1 cut(s) 1393
SstI GAGCTC 1 cut(s) 1499
StyD4I CCNGG 5 cut(s) 55, 64, 603, 802, 889
StyI CCWWGG 4 cut(s) 29, 291, 867, 1179
TaaI ACNGT 5 cut(s) 592, 664, 1862, 1997, 2170
TaiI ACGT 4 cut(s) 1143, 2072, 2076, 2153
TaqI TCGA 8 cut(s) 410, 423, 481, 540, 967, 1022, 1256, 1371
TaqII GACCGA 1 cut(s) 712
TatI WGTACW 3 cut(s) 135, 530, 664
Tru1I TTAA 8 cut(s) 303, 609, 699, 762, 863, 1104, 1146, 1611
Tru9I TTAA 8 cut(s) 303, 609, 699, 762, 863, 1104, 1146, 1611
TscAI CASTG 3 cut(s) 113, 597, 2002
TspDTI ATGAA 6 cut(s) 110, 885, 978, 1038, 1848, 1983
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 3 cut(s) 113, 597, 2002
Van91I CCANNNNNTGG 1 cut(s) 953
VneI GTGCAC 1 cut(s) 1799
VpaK11BI GGWCC 3 cut(s) 366, 724, 1261
VspI ATTAAT 1 cut(s) 762
XapI RAATTY 4 cut(s) 789, 1533, 1835, 1970
XbaI TCTAGA 1 cut(s) 742
XceI RCATGY 6 cut(s) 85, 92, 204, 698, 1172, 2194
XcmI CCANNNNNNNNNTGG 2 cut(s) 72, 158
XmaJI CCTAGG 1 cut(s) 29
XmiI GTMKAC 2 cut(s) 540, 983
XmnI GAANNNNTTC 1 cut(s) 2119
ZraI GACGTC 1 cut(s) 2151
ZrmI AGTACT 1 cut(s) 532
Zsp2I ATGCAT 2 cut(s) 202, 1378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.