Rorug03G0083100

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004926
Physical Location & Seq
Forward (+)
6455061 .. 6457871
2811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0083100.1

Sequence Viewer

Length: 1167 bp
ATGGACACCTTCCTCTTCACCTCCGAGTCTGTGAATGAGGGACACCCCGATAAGCTCTGTGATCAGATTTCCGATGCAGTGCTTGATGCCTGCCTTGAACAAGATCCTGACAGCAAAGTTGCCTGTGAGACCTGCACCAAGACCAACATGGTCATGGTCTTCGGAGAGATCACAACTAAAGCCGAAGTGGACTATGAGAAGATAGTGCGCAACACCTGTCGCAGCATTGGATTTGTTTCGGATGATGTAGGCCTTGATGCTGACAACTGCAAGGTCCTCGTGAACATTGAGCAACAAAGCCCTGATATAGCTCAGGGTGTGCATGGCCACCTCACCAAGAGGCCTGAGGAGATTGGTGCAGGAGACCAGGGTCACATGTTTGGTTATGCTACTGATGAGACCCCTGAGCTTATGCCTCTAAGCCATGTTCTTGCTACCAAGCTTGGTGCTCGTCTCACTGATGTTCGAAAGAATGGCACCTGCCCTTGGCTGAGACCTGATGGCAAGACTCAAGTCACTGTTGAGTACTACAATGACCATGGTGCTATGGTTCCAATCCGTGTCCACACCGTTCTCATTTCTACTCAGCATGATGAGACTGTTACCAATGATGAAATTGCAGCTGACCTCAAGAAACATGTCATTAAGCCAGTTGTTCCTGAGAAGTACCTAGATGAGAAGACTATCTTCCATCTCAACCCTTCAGGTCGATTTGTGATTGGTGGACCGCACGGTGATGCAGGTCTAACTGGCCGAAAGATCATCATTGACACCTATGGTGGTTGGGGAGCACATGGTGGTGGTGCCTTCTCTGGCAAGGACCCTACCAAGGTAGACAGGAGTGGTGCCTACATTGTTAGGCAGGCTGCTAAGAGCATTGTGGCCAATGGACTTGCTAGGAGGTGCATAGTGCAGGTCTCATATGCAATTGGTGTCCCTGAGCCACTATCGGTGTTTGTTGACACTTACGGGACTGGAAAGATTCCGGACAAGGAGATTCTGAAGTTAGTGAAGGAGGCCTTTGATTTTAGGCCTGGAATGATTGCCATTAACTTGGATCTCAAAAGAGGAAGGTATTTGAAGACGGCTGCTTATGGACACTTTGGTAGAGATGACCCTGACTTTTCGTGGGAAGTGGTCAAGTCCTTGAAGTGGGAAAAGGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3741 DUF4378

Protein Analysis

388

Amino Acids

42.57

Weight (kDa)

5.73

Isoelectric Point (pI)

24.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 4 - 101 2.3e-43 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 117 - 238 3.3e-47 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 240 - 378 8e-63 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 488
Acc16I TGCGCA 1 cut(s) 209
Acc36I ACCTGC 4 cut(s) 140, 488, 731, 904
AccB1I GGYRCC 3 cut(s) 476, 803, 845
AccI GTMKAC 1 cut(s) 834
AccIII TCCGGA 1 cut(s) 985
AciI CCGC 1 cut(s) 728
AclWI GGATC 2 cut(s) 98, 1065
AcoI YGGCCR 3 cut(s) 325, 751, 882
AcuI CTGAAG 2 cut(s) 687, 1022
AdeI CACNNNGTG 1 cut(s) 797
AfaI GTAC 2 cut(s) 527, 668
AfiI CCNNNNNNNGG 3 cut(s) 486, 829, 1152
AflIII ACRYGT 2 cut(s) 375, 637
AgsI TTSAA 3 cut(s) 98, 1081, 1150
AjnI CCWGG 2 cut(s) 366, 1033
AluBI AGCT 5 cut(s) 55, 311, 409, 442, 623
AluI AGCT 5 cut(s) 55, 311, 409, 442, 623
Alw21I GWGCWC 2 cut(s) 451, 793
Alw26I GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 922
AlwI GGATC 2 cut(s) 98, 1065
Aor13HI TCCGGA 1 cut(s) 985
AoxI GGCC 7 cut(s) 250, 325, 341, 751, 882, 1017, 1031
ApeKI GCWGC 4 cut(s) 222, 620, 866, 1088
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 3 cut(s) 274, 725, 820
AsuHPI GGTGA 3 cut(s) 10, 325, 746
AsuII TTCGAA 1 cut(s) 466
AvaII GGWCC 3 cut(s) 274, 725, 820
AxyI CCTNAGG 1 cut(s) 345
BalI TGGCCA 2 cut(s) 327, 884
BanI GGYRCC 3 cut(s) 476, 803, 845
BauI CACGAG 1 cut(s) 278
BbsI GAAGAC 3 cut(s) 151, 686, 1088
Bbv12I GWGCWC 2 cut(s) 451, 793
BbvI GCAGC 4 cut(s) 234, 632, 853, 1075
BccI CCATC 2 cut(s) 494, 699
BceAI ACGGC 1 cut(s) 1101
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BciT130I CCWGG 2 cut(s) 368, 1035
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 922
BfaI CTAG 2 cut(s) 671, 897
BfuAI ACCTGC 4 cut(s) 140, 488, 731, 904
BisI GCNGC 4 cut(s) 223, 621, 867, 1089
BlsI GCNGC 4 cut(s) 224, 622, 868, 1090
BmcAI AGTACT 1 cut(s) 527
Bme1390I CCNGG 2 cut(s) 368, 1035
Bme18I GGWCC 3 cut(s) 274, 725, 820
BmgT120I GGNCC 3 cut(s) 274, 725, 820
BmiI GGNNCC 5 cut(s) 478, 552, 805, 822, 847
BmrFI CCNGG 2 cut(s) 368, 1035
BmsI GCATC 4 cut(s) 64, 76, 247, 727
BoxI GACNNNNGTC 2 cut(s) 369, 512
BpiI GAAGAC 3 cut(s) 151, 686, 1088
Bpu10I CCTNAGC 3 cut(s) 312, 405, 939
Bpu14I TTCGAA 1 cut(s) 466
BpuEI CTTGAG 2 cut(s) 495, 614
BsaI GGTCTC 5 cut(s) 122, 357, 392, 487, 922
BsaJI CCNNGG 4 cut(s) 367, 485, 538, 828
BsaWI WCCGGW 1 cut(s) 985
Bsc4I CCNNNNNNNGG 3 cut(s) 486, 829, 1152
Bse1I ACTGG 3 cut(s) 650, 754, 979
Bse21I CCTNAGG 1 cut(s) 345
BseAI TCCGGA 1 cut(s) 985
BseBI CCWGG 2 cut(s) 368, 1035
BseDI CCNNGG 4 cut(s) 367, 485, 538, 828
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 3 cut(s) 486, 829, 1152
BseMII CTCAG 7 cut(s) 326, 336, 396, 482, 599, 651, 930
BseNI ACTGG 3 cut(s) 650, 754, 979
BseRI GAGGAG 1 cut(s) 362
BseXI GCAGC 4 cut(s) 234, 632, 853, 1075
BsgI GTGCAG 3 cut(s) 118, 378, 932
BshFI GGCC 7 cut(s) 252, 327, 343, 753, 884, 1019, 1033
BshNI GGYRCC 3 cut(s) 476, 803, 845
BsiHKAI GWGCWC 2 cut(s) 451, 793
BsiSI CCGG 1 cut(s) 986
BslFI GGGAC 3 cut(s) 54, 920, 985
BslI CCNNNNNNNGG 3 cut(s) 486, 829, 1152
BsmAI GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 922
BsmBI CGTCTC 1 cut(s) 458
BsmFI GGGAC 3 cut(s) 54, 920, 985
BsnI GGCC 7 cut(s) 252, 327, 343, 753, 884, 1019, 1033
Bso31I GGTCTC 5 cut(s) 122, 357, 392, 487, 922
Bsp119I TTCGAA 1 cut(s) 466
Bsp1286I GDGCHC 2 cut(s) 451, 793
Bsp13I TCCGGA 1 cut(s) 985
Bsp143I GATC 5 cut(s) 61, 103, 168, 759, 1057
Bsp19I CCATGG 1 cut(s) 538
BspACI CCGC 1 cut(s) 728
BspANI GGCC 7 cut(s) 252, 327, 343, 753, 884, 1019, 1033
BspCNI CTCAG 7 cut(s) 325, 337, 397, 483, 598, 652, 931
BspEI TCCGGA 1 cut(s) 985
BspLI GGNNCC 5 cut(s) 478, 552, 805, 822, 847
BspMI ACCTGC 4 cut(s) 140, 488, 731, 904
BspPI GGATC 2 cut(s) 98, 1065
BspT104I TTCGAA 1 cut(s) 466
BspT107I GGYRCC 3 cut(s) 476, 803, 845
BspTNI GGTCTC 5 cut(s) 122, 357, 392, 487, 922
BsrI ACTGG 3 cut(s) 650, 754, 979
BssECI CCNNGG 4 cut(s) 367, 485, 538, 828
BssMI GATC 5 cut(s) 61, 103, 168, 759, 1057
BssSI CACGAG 1 cut(s) 278
BssT1I CCWWGG 3 cut(s) 485, 538, 828
Bst2BI CACGAG 1 cut(s) 278
Bst2UI CCWGG 2 cut(s) 368, 1035
Bst4CI ACNGT 4 cut(s) 520, 571, 601, 734
Bst6I CTCTTC 1 cut(s) 20
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 2 cut(s) 91, 864
BstDEI CTNAG 9 cut(s) 312, 345, 405, 419, 491, 585, 660, 870, 939
BstDSI CCRYGG 1 cut(s) 538
BstF5I GGATG 1 cut(s) 247
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 5 cut(s) 64, 106, 171, 762, 1060
BstMAI GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 922
BstMBI GATC 5 cut(s) 61, 103, 168, 759, 1057
BstNI CCWGG 2 cut(s) 368, 1035
BstNSI RCATGY 2 cut(s) 379, 641
BstPAI GACNNNNGTC 2 cut(s) 369, 512
BstSCI CCNGG 2 cut(s) 366, 1033
BstV1I GCAGC 4 cut(s) 234, 632, 853, 1075
BstV2I GAAGAC 3 cut(s) 151, 686, 1088
BstX2I RGATCY 2 cut(s) 103, 1057
BstXI CCANNNNNNTGG 1 cut(s) 1054
BstYI RGATCY 2 cut(s) 103, 1057
Bsu36I CCTNAGG 1 cut(s) 345
BsuRI GGCC 7 cut(s) 252, 327, 343, 753, 884, 1019, 1033
BtgI CCRYGG 1 cut(s) 538
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 3 cut(s) 84, 456, 516
BveI ACCTGC 4 cut(s) 140, 488, 731, 904
Cac8I GCNNGC 2 cut(s) 91, 864
CfoI GCGC 1 cut(s) 210
Cfr13I GGNCC 3 cut(s) 274, 725, 820
Csp6I GTAC 2 cut(s) 526, 667
CviAII CATG 9 cut(s) 148, 154, 323, 376, 425, 539, 590, 638, 794
CviQI GTAC 2 cut(s) 526, 667
DdeI CTNAG 9 cut(s) 312, 345, 405, 419, 491, 585, 660, 870, 939
DpnI GATC 5 cut(s) 63, 105, 170, 761, 1059
DpnII GATC 5 cut(s) 61, 103, 168, 759, 1057
DraIII CACNNNGTG 1 cut(s) 797
EaeI YGGCCR 3 cut(s) 325, 751, 882
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 485, 538, 828
Eco147I AGGCCT 4 cut(s) 252, 343, 1019, 1033
Eco31I GGTCTC 5 cut(s) 122, 357, 392, 487, 922
Eco47I GGWCC 3 cut(s) 274, 725, 820
Eco57I CTGAAG 2 cut(s) 687, 1022
Eco81I CCTNAGG 1 cut(s) 345
EcoO109I RGGNCCY 2 cut(s) 274, 820
EcoRII CCWGG 2 cut(s) 366, 1033
EcoT14I CCWWGG 3 cut(s) 485, 538, 828
ErhI CCWWGG 3 cut(s) 485, 538, 828
Esp3I CGTCTC 1 cut(s) 458
FaeI CATG 9 cut(s) 151, 157, 326, 379, 428, 542, 593, 641, 797
FalI AAGNNNNNCTT 4 cut(s) 671, 703, 1004, 1036
FaqI GGGAC 3 cut(s) 54, 920, 985
FatI CATG 9 cut(s) 147, 153, 322, 375, 424, 538, 589, 637, 793
FauNDI CATATG 1 cut(s) 922
FbaI TGATCA 1 cut(s) 61
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 4 cut(s) 223, 621, 867, 1089
FokI GGATG 1 cut(s) 254
Fsp4HI GCNGC 4 cut(s) 223, 621, 867, 1089
FspBI CTAG 2 cut(s) 671, 897
FspI TGCGCA 1 cut(s) 209
GlaI GCGC 1 cut(s) 209
GluI GCNGC 4 cut(s) 223, 621, 867, 1089
HaeIII GGCC 7 cut(s) 252, 327, 343, 753, 884, 1019, 1033
HapII CCGG 1 cut(s) 986
HhaI GCGC 1 cut(s) 210
Hin1II CATG 9 cut(s) 151, 157, 326, 379, 428, 542, 593, 641, 797
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HincII GTYRAC 1 cut(s) 961
HindII GTYRAC 1 cut(s) 961
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 4 cut(s) 26, 508, 982, 997
HpaII CCGG 1 cut(s) 986
HphI GGTGA 3 cut(s) 10, 325, 746
Hpy166II GTNNAC 6 cut(s) 190, 283, 565, 725, 835, 961
Hpy188I TCNGA 6 cut(s) 25, 66, 73, 164, 241, 1002
Hpy188III TCNNGA 5 cut(s) 107, 280, 631, 659, 986
Hpy8I GTNNAC 6 cut(s) 190, 283, 565, 725, 835, 961
HpyAV CCTTC 5 cut(s) 19, 711, 817, 1006, 1065
HpyCH4III ACNGT 4 cut(s) 520, 571, 601, 734
HpyF3I CTNAG 9 cut(s) 312, 345, 405, 419, 491, 585, 660, 870, 939
Hsp92II CATG 9 cut(s) 151, 157, 326, 379, 428, 542, 593, 641, 797
HspAI GCGC 1 cut(s) 208
Kpn2I TCCGGA 1 cut(s) 985
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 5 cut(s) 61, 103, 168, 759, 1057
LmnI GCTCC 1 cut(s) 788
Lsp1109I GCAGC 4 cut(s) 234, 632, 853, 1075
LweI GCATC 4 cut(s) 64, 76, 247, 727
MaeI CTAG 2 cut(s) 671, 897
MaeIII GTNAC 3 cut(s) 371, 514, 601
MalI GATC 5 cut(s) 63, 105, 170, 761, 1059
MboI GATC 5 cut(s) 61, 103, 168, 759, 1057
MboII GAAGA 6 cut(s) 7, 151, 211, 679, 691, 1093
MfeI CAATTG 1 cut(s) 927
MflI RGATCY 2 cut(s) 103, 1057
MhlI GDGCHC 2 cut(s) 451, 793
MlsI TGGCCA 2 cut(s) 327, 884
MluCI AATT 2 cut(s) 615, 927
MluNI TGGCCA 2 cut(s) 327, 884
MlyI GAGTC 2 cut(s) 35, 502
Mox20I TGGCCA 2 cut(s) 327, 884
MroI TCCGGA 1 cut(s) 985
MscI TGGCCA 2 cut(s) 327, 884
MseI TTAA 2 cut(s) 645, 1050
MslI CAYNNNNRTG 3 cut(s) 152, 735, 798
Msp20I TGGCCA 2 cut(s) 327, 884
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 1 cut(s) 986
MspR9I CCNGG 2 cut(s) 368, 1035
MunI CAATTG 1 cut(s) 927
MvaI CCWGG 2 cut(s) 368, 1035
NcoI CCATGG 1 cut(s) 538
NdeI CATATG 1 cut(s) 922
NdeII GATC 5 cut(s) 61, 103, 168, 759, 1057
NlaIII CATG 9 cut(s) 151, 157, 326, 379, 428, 542, 593, 641, 797
NlaIV GGNNCC 5 cut(s) 478, 552, 805, 822, 847
NmuCI GTSAC 2 cut(s) 371, 514
NsbI TGCGCA 1 cut(s) 209
NspI RCATGY 2 cut(s) 379, 641
NspV TTCGAA 1 cut(s) 466
PaqCI CACCTGC 1 cut(s) 488
PceI AGGCCT 4 cut(s) 252, 343, 1019, 1033
PciI ACATGT 2 cut(s) 375, 637
PfeI GAWTC 2 cut(s) 982, 997
PkrI GCNGC 4 cut(s) 224, 622, 868, 1090
PleI GAGTC 2 cut(s) 34, 502
PpsI GAGTC 2 cut(s) 34, 502
PpuMI RGGWCCY 2 cut(s) 274, 820
PscI ACATGT 2 cut(s) 375, 637
PshAI GACNNNNGTC 2 cut(s) 369, 512
Psp5II RGGWCCY 2 cut(s) 274, 820
Psp6I CCWGG 2 cut(s) 366, 1033
PspGI CCWGG 2 cut(s) 366, 1033
PspN4I GGNNCC 5 cut(s) 478, 552, 805, 822, 847
PspPI GGNCC 3 cut(s) 274, 725, 820
PspPPI RGGWCCY 2 cut(s) 274, 820
PsuI RGATCY 2 cut(s) 103, 1057
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 2 cut(s) 527, 668
RsaNI GTAC 2 cut(s) 526, 667
RseI CAYNNNNRTG 3 cut(s) 152, 735, 798
SaqAI TTAA 2 cut(s) 645, 1050
SatI GCNGC 4 cut(s) 223, 621, 867, 1089
Sau3AI GATC 5 cut(s) 61, 103, 168, 759, 1057
Sau96I GGNCC 3 cut(s) 274, 725, 820
ScaI AGTACT 1 cut(s) 527
SchI GAGTC 2 cut(s) 35, 502
ScrFI CCNGG 2 cut(s) 368, 1035
SduI GDGCHC 2 cut(s) 451, 793
SfaNI GCATC 4 cut(s) 64, 76, 247, 727
SfuI TTCGAA 1 cut(s) 466
SinI GGWCC 3 cut(s) 274, 725, 820
SmiMI CAYNNNNRTG 3 cut(s) 152, 735, 798
SmlI CTYRAG 2 cut(s) 510, 629
SmoI CTYRAG 2 cut(s) 510, 629
Sse9I AATT 2 cut(s) 615, 927
SseBI AGGCCT 4 cut(s) 252, 343, 1019, 1033
SsiI CCGC 1 cut(s) 728
SspMI CTAG 2 cut(s) 671, 897
StuI AGGCCT 4 cut(s) 252, 343, 1019, 1033
StyD4I CCNGG 2 cut(s) 366, 1033
StyI CCWWGG 3 cut(s) 485, 538, 828
TaaI ACNGT 4 cut(s) 520, 571, 601, 734
TaqI TCGA 2 cut(s) 466, 709
TasI AATT 2 cut(s) 615, 927
TatI WGTACW 1 cut(s) 525
TfiI GAWTC 2 cut(s) 982, 997
Tru1I TTAA 2 cut(s) 645, 1050
Tru9I TTAA 2 cut(s) 645, 1050
TscAI CASTG 3 cut(s) 84, 463, 523
TseFI GTSAC 2 cut(s) 371, 514
TseI GCWGC 4 cut(s) 222, 620, 866, 1088
Tsp45I GTSAC 2 cut(s) 371, 514
TspDTI ATGAA 1 cut(s) 627
TspGWI ACGGA 1 cut(s) 548
TspRI CASTG 3 cut(s) 84, 463, 523
VpaK11BI GGWCC 3 cut(s) 274, 725, 820
XceI RCATGY 2 cut(s) 379, 641
XcmI CCANNNNNNNNNTGG 2 cut(s) 145, 151
XmiI GTMKAC 1 cut(s) 834
XspI CTAG 2 cut(s) 671, 897
ZrmI AGTACT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.