MD12G1141300.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004926
Physical Location & Seq
Reverse (-)
21935759 .. 21938278
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1141300.v1.1.491

Sequence Viewer

Length: 2094 bp
ATGGGTAACGCAGTCGTAAAAACCGGCCGTTGTTTCTGCACCGCCGGAGAAATCTCCCAGCGACACGACAACTCCGTCGTTTCGTCGGACCCGCTCGACGAGGGCCTAGGCCATTCCTTCTGCTACTTCCGGCCCGACTCCTCCGATACGACGTCGTCTAAAGTCTTGTCCGAAGAGCCGAACCCGCCAACGACGACATTCCGGTCAATCTCCGGCGCCTCCGTCAGCGCCAACACATTCACGCCGCTCTCGACTTCTCTCTGCGAGCTCTACCCGTACAGCGCCGGCTGCGATCGGGCGGCGAAGTTCGAGAGCTCGAACTCGTTCGCCTCCATTCCTCTGCAGCCGGTTCCTCGGAAGTCATACCAGGGAGTTTCCGGGTCGAGTCCGATCGAGAGAGGGTTCTTGTCAGGTCCCATAGAGCGAGGCTACTTGTCGGGCCCGATTGATCACGGGGTGTATTCGGGTCCGATCGAGAAAGAGTGCGACAAATTCTCGAGAAGCCTTTCTTTTGGGATTGAAACGAAACCAAAAATAAGGAGCTTGATGAAGATTTTCAGAAGAGCGGTTTTGGGTGCCATGCTTCGGGGGCAGAAATCGAGCGTTTCGCCATTAAAAGGCAACATTTCAGTGAAAGATTCGGATTCGGAGAAGGTGGGGGAGAATGGGACTAGCAGTAGTATTAATTTGAGCAGCCATGTGAGTTTGAGTAATGAGAATGAGGATGGTGGGAGTGGACATTACTCGATGGGAAGCCAGAATCTTCAATGGGCTCAAGGGAAAGCCGGCGAGGATCGGGTTCATGTGGTGGTTTCGGAGGAACATAGCTGGGTTTTTGTGGGGATTTATGATGGGTTTAATGGCCCTGATGCTCCTGATTACTTGCTTTCCAATCTTTACTCTGCTGTGCACAAGGAACTCAAAGGGTTGCTTTGGAATGACAAGTTCGAAATTGAAATTGAACCCGAGTTAAATTCATTGAATACGGATGTGGGTTCGGTTTCTTTGAAGGAAACTGATCGAACCCAGATGGGGAATCGGATGCCTGACGGTGTCGATGGGGAGAAACATGCCTTAGAAAATGGAGAAATGGAAGGAAAGGCTAATTCAATAAGAAAACAGAGTAATGGGTTGGAAAATAAGTTGGAGGGGACTCGGAGGAGGTGGACGTGTGAATGGGACAGAGAAAGGCCAGAGCTTGATCGTAAGTTACAGCAGAATTTAAAGCGTCCAGTGTCGAATGGTGTGGCCGGTGTTAACCCTTCTGATGTTCTTAAAGCACTTTCGGAGGCACTGAGGAAGACAGAGGATGCATTTTTGGACACTGCAGATAAGGTGGTGACTGAGAATCCTGAGTTGGCCTTGATGGGTTCTTGTGTTTTGGTAATGCTAATGAAGGGAGATGATGTTTACTTGTTGAATGTTGGGGACAGTAGGGCGGTTTTAGCTCGGAAATCGGAACAAAATGTTCGGCGGAACTTGGATCGGATTAGTGAGGATGAGCCTTCATTTGATTCATCAAATGCTTATGAAAGTTACAGTTTAAAATATTTGACTGCACTTCAGCTCACCATGGATCACAGCACACATGTGAAAGAGGAAGTACAAAGAATAAAGAAAGAACATCCAGACGATGCTTCTGCGATAACAAATGACAGAGTGAAAGGTTATTTGAAGGTCACTCGAGCGTTTGGAGCTGGCTTTCTCAAACAGCCAAAGTGGAATGATGCACTGCTAGAGAGGTTTAGACTCAAATATGTTGGGACTGCCCCCTACCTCACCTGCAATCCATCCCTCTACCACCACAAACTCAGCCCGAGAGACCGGCTCTTAATATTGTCTTCTGATGGACTCTACCAGTACTTCACTAATGAAGAAGCCGTCTCTGAAGTAGAATTGTTCATCTCTTCATTTCCCGATGGAGATCCTGCGCAACATCTCATCGAAGAAGTGTTGTTTCGAGCAGCAAAGAAAGCTGGAATGGCATTCCATGAGCTGCTAGATATCCCACAAGGGGAACGCCGACGGTACCATGACGATGTTTCCATCATTGTGATTTCGTTGGAAGGAAGAATATGGAGATCATCAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0071944 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

698

Amino Acids

77.28

Weight (kDa)

5.75

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 423 - 639 5.4e-26 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1790
AasI GACNNNNNNGTC 2 cut(s) 74, 202
AatII GACGTC 1 cut(s) 155
Acc16I TGCGCA 1 cut(s) 1932
Acc36I ACCTGC 1 cut(s) 1790
Acc65I GGTACC 1 cut(s) 2028
AccB1I GGYRCC 3 cut(s) 215, 575, 2028
AccBSI CCGCTC 3 cut(s) 94, 247, 566
AciI CCGC 8 cut(s) 42, 92, 185, 245, 299, 566, 1439, 1474
AclWI GGATC 4 cut(s) 801, 1491, 1584, 1919
AcoI YGGCCR 2 cut(s) 25, 1248
AcsI RAATTY 3 cut(s) 491, 973, 1219
AcuI CTGAAG 2 cut(s) 1547, 1908
AcyI GRCGYC 2 cut(s) 152, 216
AdeI CACNNNGTG 1 cut(s) 457
AfaI GTAC 4 cut(s) 278, 1605, 1862, 2030
AfiI CCNNNNNNNGG 4 cut(s) 585, 617, 1032, 2014
AflIII ACRYGT 2 cut(s) 1169, 1588
AgsI TTSAA 9 cut(s) 521, 767, 956, 962, 982, 1009, 1110, 1420, 1675
AjiI CACGTC 1 cut(s) 1170
AjnI CCWGG 1 cut(s) 366
AjuI GAANNNNNNNTTGG 2 cut(s) 1340, 1372
AleI CACNNNNGTG 1 cut(s) 1589
Alw21I GWGCWC 3 cut(s) 270, 317, 912
Alw26I GTCTC 2 cut(s) 1815, 1888
Alw44I GTGCAC 1 cut(s) 908
AlwI GGATC 4 cut(s) 801, 1491, 1584, 1919
Ama87I CYCGRG 4 cut(s) 496, 965, 1683, 1816
AoxI GGCC 9 cut(s) 25, 103, 109, 131, 439, 862, 1190, 1248, 1359
ApaI GGGCCC 1 cut(s) 443
ApaLI GTGCAC 1 cut(s) 908
ApeKI GCWGC 5 cut(s) 288, 343, 693, 1964, 1996
ApoI RAATTY 3 cut(s) 491, 973, 1219
AseI ATTAAT 1 cut(s) 684
Asp700I GAANNNNTTC 3 cut(s) 323, 505, 554
Asp718I GGTACC 1 cut(s) 2028
AspA2I CCTAGG 1 cut(s) 106
AspLEI GCGC 4 cut(s) 218, 230, 284, 1933
AspS9I GGNCC 8 cut(s) 88, 103, 132, 413, 439, 440, 467, 863
AsuC2I CCSGG 1 cut(s) 379
AsuHPI GGTGA 3 cut(s) 1351, 1561, 1771
AsuII TTCGAA 1 cut(s) 948
AvaI CYCGRG 4 cut(s) 496, 965, 1683, 1816
AvaII GGWCC 3 cut(s) 88, 413, 467
AvrII CCTAGG 1 cut(s) 106
BaeGI GKGCMC 2 cut(s) 443, 912
BanI GGYRCC 3 cut(s) 215, 575, 2028
BanII GRGCYC 4 cut(s) 270, 317, 443, 775
BbsI GAAGAC 2 cut(s) 1307, 1833
Bbv12I GWGCWC 3 cut(s) 270, 317, 912
BbvI GCAGC 5 cut(s) 275, 355, 705, 1976, 1983
BceAI ACGGC 2 cut(s) 12, 1865
BcgI CGANNNNNNTGC 2 cut(s) 1622, 1656
BciT130I CCWGG 1 cut(s) 368
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 1 cut(s) 379
BcoDI GTCTC 2 cut(s) 1815, 1888
BfaI CTAG 4 cut(s) 107, 672, 1736, 2000
BfmI CTRYAG 2 cut(s) 341, 1326
BfoI RGCGCY 3 cut(s) 219, 231, 285
BfuAI ACCTGC 1 cut(s) 1790
BisI GCNGC 7 cut(s) 245, 289, 300, 344, 694, 1965, 1997
BlnI CCTAGG 1 cut(s) 106
BlsI GCNGC 7 cut(s) 246, 290, 301, 345, 695, 1966, 1998
BmcAI AGTACT 1 cut(s) 1862
Bme1390I CCNGG 2 cut(s) 368, 379
Bme18I GGWCC 3 cut(s) 88, 413, 467
BmeT110I CYCGRG 4 cut(s) 496, 965, 1683, 1816
BmgBI CACGTC 1 cut(s) 1170
BmgT120I GGNCC 8 cut(s) 88, 103, 132, 413, 439, 440, 467, 863
BmiI GGNNCC 8 cut(s) 90, 217, 351, 415, 441, 468, 577, 2030
BmrFI CCNGG 2 cut(s) 368, 379
BmsI GCATC 5 cut(s) 859, 1032, 1300, 1624, 1717
BpiI GAAGAC 2 cut(s) 1307, 1833
BplI GAGNNNNNCTC 2 cut(s) 1812, 1844
Bpu14I TTCGAA 1 cut(s) 948
BpuEI CTTGAG 1 cut(s) 759
BpuMI CCSGG 1 cut(s) 379
BsaBI GATNNNNATC 1 cut(s) 1923
BsaHI GRCGYC 2 cut(s) 152, 216
BsaI GGTCTC 1 cut(s) 1815
BsaJI CCNNGG 4 cut(s) 106, 353, 367, 1572
BsaWI WCCGGW 1 cut(s) 201
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 1392, 1422
Bsc4I CCNNNNNNNGG 4 cut(s) 585, 617, 1032, 2014
Bse118I RCCGGY 6 cut(s) 23, 284, 346, 785, 1250, 1824
Bse1I ACTGG 2 cut(s) 1232, 1858
Bse8I GATNNNNATC 1 cut(s) 1923
BseBI CCWGG 1 cut(s) 368
BseDI CCNNGG 4 cut(s) 106, 353, 367, 1572
BseGI GGATG 7 cut(s) 730, 994, 1047, 1315, 1504, 1624, 1790
BseJI GATNNNNATC 1 cut(s) 1923
BseLI CCNNNNNNNGG 4 cut(s) 585, 617, 1032, 2014
BseMII CTCAG 4 cut(s) 1286, 1335, 1344, 1825
BseNI ACTGG 2 cut(s) 1232, 1858
BseRI GAGGAG 2 cut(s) 130, 1174
BseSI GKGCMC 2 cut(s) 443, 912
BseX3I CGGCCG 1 cut(s) 25
BseXI GCAGC 5 cut(s) 275, 355, 705, 1976, 1983
BseYI CCCAGC 2 cut(s) 57, 828
BsgI GTGCAG 2 cut(s) 22, 1542
Bsh1285I CGRYCG 4 cut(s) 28, 295, 393, 474
BshFI GGCC 9 cut(s) 27, 105, 111, 133, 441, 864, 1192, 1250, 1361
BshNI GGYRCC 3 cut(s) 215, 575, 2028
BsiEI CGRYCG 4 cut(s) 28, 295, 393, 474
BsiHKAI GWGCWC 3 cut(s) 270, 317, 912
BsiHKCI CYCGRG 4 cut(s) 496, 965, 1683, 1816
BslFI GGGAC 6 cut(s) 399, 682, 1165, 1193, 1442, 1777
BslI CCNNNNNNNGG 4 cut(s) 585, 617, 1032, 2014
BsmAI GTCTC 2 cut(s) 1815, 1888
BsmBI CGTCTC 1 cut(s) 1888
BsmFI GGGAC 6 cut(s) 399, 682, 1165, 1193, 1442, 1777
BsmI GAATGC 1 cut(s) 1985
BsnI GGCC 9 cut(s) 27, 105, 111, 133, 441, 864, 1192, 1250, 1361
Bso31I GGTCTC 1 cut(s) 1815
BsoBI CYCGRG 4 cut(s) 496, 965, 1683, 1816
Bsp119I TTCGAA 1 cut(s) 948
Bsp120I GGGCCC 1 cut(s) 439
Bsp1286I GDGCHC 5 cut(s) 270, 317, 443, 775, 912
Bsp19I CCATGG 1 cut(s) 1572
BspACI CCGC 8 cut(s) 42, 92, 185, 245, 299, 566, 1439, 1474
BspANI GGCC 9 cut(s) 27, 105, 111, 133, 441, 864, 1192, 1250, 1361
BspCNI CTCAG 4 cut(s) 1287, 1336, 1345, 1824
BspLI GGNNCC 8 cut(s) 90, 217, 351, 415, 441, 468, 577, 2030
BspMAI CTGCAG 2 cut(s) 345, 1330
BspMI ACCTGC 1 cut(s) 1790
BspPI GGATC 4 cut(s) 801, 1491, 1584, 1919
BspQI GCTCTTC 2 cut(s) 168, 556
BspT104I TTCGAA 1 cut(s) 948
BspT107I GGYRCC 3 cut(s) 215, 575, 2028
BspTNI GGTCTC 1 cut(s) 1815
BsrBI CCGCTC 3 cut(s) 94, 247, 566
BsrFI RCCGGY 6 cut(s) 23, 284, 346, 785, 1250, 1824
BsrI ACTGG 2 cut(s) 1232, 1858
BssAI RCCGGY 6 cut(s) 23, 284, 346, 785, 1250, 1824
BssECI CCNNGG 4 cut(s) 106, 353, 367, 1572
BssNI GRCGYC 2 cut(s) 152, 216
BssT1I CCWWGG 2 cut(s) 106, 1572
Bst2UI CCWGG 1 cut(s) 368
Bst4CI ACNGT 4 cut(s) 1052, 1433, 1541, 2028
Bst6I CTCTTC 3 cut(s) 168, 556, 1912
BstACI GRCGYC 2 cut(s) 152, 216
BstBI TTCGAA 1 cut(s) 948
BstC8I GCNNGC 4 cut(s) 266, 286, 787, 1699
BstDEI CTNAG 5 cut(s) 1075, 1295, 1344, 1353, 1811
BstDSI CCRYGG 1 cut(s) 1572
BstF5I GGATG 7 cut(s) 730, 994, 1047, 1315, 1504, 1624, 1790
BstH2I RGCGCY 3 cut(s) 219, 231, 285
BstHHI GCGC 4 cut(s) 218, 230, 284, 1933
BstMAI GTCTC 2 cut(s) 1815, 1888
BstMCI CGRYCG 4 cut(s) 28, 295, 393, 474
BstMWI GCNNNNNNNGC 7 cut(s) 184, 288, 589, 1445, 1694, 1973, 1982
BstNI CCWGG 1 cut(s) 368
BstNSI RCATGY 2 cut(s) 1073, 1592
BstSCI CCNGG 2 cut(s) 366, 377
BstSFI CTRYAG 2 cut(s) 341, 1326
BstSLI GKGCMC 2 cut(s) 443, 912
BstV1I GCAGC 5 cut(s) 275, 355, 705, 1976, 1983
BstV2I GAAGAC 2 cut(s) 1307, 1833
BstX2I RGATCY 1 cut(s) 1924
BstYI RGATCY 1 cut(s) 1924
BstZI CGGCCG 1 cut(s) 25
BsuRI GGCC 9 cut(s) 27, 105, 111, 133, 441, 864, 1192, 1250, 1361
BtgI CCRYGG 1 cut(s) 1572
BtrI CACGTC 1 cut(s) 1170
BtsCI GGATG 7 cut(s) 730, 994, 1047, 1315, 1504, 1624, 1790
BtsI GCAGTG 2 cut(s) 1323, 1730
BtsIMutI CAGTG 6 cut(s) 636, 1239, 1292, 1323, 1730, 2094
BveI ACCTGC 1 cut(s) 1790
Cac8I GCNNGC 4 cut(s) 266, 286, 787, 1699
CfoI GCGC 4 cut(s) 218, 230, 284, 1933
Cfr10I RCCGGY 6 cut(s) 23, 284, 346, 785, 1250, 1824
Cfr13I GGNCC 8 cut(s) 88, 103, 132, 413, 439, 440, 467, 863
CseI GACGC 1 cut(s) 1217
Csp6I GTAC 4 cut(s) 277, 1604, 1861, 2029
CviAII CATG 8 cut(s) 580, 698, 803, 1070, 1573, 1589, 1991, 2033
CviQI GTAC 4 cut(s) 277, 1604, 1861, 2029
DdeI CTNAG 5 cut(s) 1075, 1295, 1344, 1353, 1811
DinI GGCGCC 1 cut(s) 217
DraI TTTAAA 2 cut(s) 1224, 1545
DraIII CACNNNGTG 1 cut(s) 457
DrdI GACNNNNNNGTC 2 cut(s) 74, 202
DseDI GACNNNNNNGTC 2 cut(s) 74, 202
EaeI YGGCCR 2 cut(s) 25, 1248
EagI CGGCCG 1 cut(s) 25
Eam1104I CTCTTC 3 cut(s) 168, 556, 1912
EarI CTCTTC 3 cut(s) 168, 556, 1912
EciI GGCGGA 1 cut(s) 1489
Ecl136II GAGCTC 2 cut(s) 268, 315
EclXI CGGCCG 1 cut(s) 25
Eco130I CCWWGG 2 cut(s) 106, 1572
Eco24I GRGCYC 4 cut(s) 270, 317, 443, 775
Eco31I GGTCTC 1 cut(s) 1815
Eco32I GATATC 1 cut(s) 2005
Eco47I GGWCC 3 cut(s) 88, 413, 467
Eco52I CGGCCG 1 cut(s) 25
Eco53kI GAGCTC 2 cut(s) 268, 315
Eco57I CTGAAG 2 cut(s) 1547, 1908
Eco88I CYCGRG 4 cut(s) 496, 965, 1683, 1816
EcoICRI GAGCTC 2 cut(s) 268, 315
EcoO109I RGGNCCY 2 cut(s) 103, 413
EcoRII CCWGG 1 cut(s) 366
EcoRV GATATC 1 cut(s) 2005
EcoT14I CCWWGG 2 cut(s) 106, 1572
EcoT22I ATGCAT 1 cut(s) 1315
EcoT38I GRGCYC 4 cut(s) 270, 317, 443, 775
EgeI GGCGCC 1 cut(s) 217
EheI GGCGCC 1 cut(s) 217
ErhI CCWWGG 2 cut(s) 106, 1572
Esp3I CGTCTC 1 cut(s) 1888
FaeI CATG 8 cut(s) 583, 701, 806, 1073, 1576, 1592, 1994, 2036
FalI AAGNNNNNCTT 4 cut(s) 493, 525, 915, 947
FaqI GGGAC 6 cut(s) 399, 682, 1165, 1193, 1442, 1777
FatI CATG 8 cut(s) 579, 697, 802, 1069, 1572, 1588, 1990, 2032
FauI CCCGC 2 cut(s) 99, 192
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 7 cut(s) 245, 289, 300, 344, 694, 1965, 1997
FokI GGATG 7 cut(s) 737, 1001, 1054, 1322, 1511, 1611, 1777
FriOI GRGCYC 4 cut(s) 270, 317, 443, 775
Fsp4HI GCNGC 7 cut(s) 245, 289, 300, 344, 694, 1965, 1997
FspBI CTAG 4 cut(s) 107, 672, 1736, 2000
FspI TGCGCA 1 cut(s) 1932
GlaI GCGC 4 cut(s) 217, 229, 283, 1932
GluI GCNGC 7 cut(s) 245, 289, 300, 344, 694, 1965, 1997
GsaI CCCAGC 2 cut(s) 61, 832
HaeII RGCGCY 3 cut(s) 219, 231, 285
HaeIII GGCC 9 cut(s) 27, 105, 111, 133, 441, 864, 1192, 1250, 1361
HgaI GACGC 1 cut(s) 1217
HhaI GCGC 4 cut(s) 218, 230, 284, 1933
Hin1I GRCGYC 2 cut(s) 152, 216
Hin1II CATG 8 cut(s) 583, 701, 806, 1073, 1576, 1592, 1994, 2036
Hin6I GCGC 4 cut(s) 216, 228, 282, 1931
HinP1I GCGC 4 cut(s) 216, 228, 282, 1931
HincII GTYRAC 1 cut(s) 1258
HindII GTYRAC 1 cut(s) 1258
HpaI GTTAAC 1 cut(s) 1258
HphI GGTGA 3 cut(s) 1351, 1561, 1771
Hpy166II GTNNAC 5 cut(s) 737, 910, 1167, 1258, 1411
Hpy8I GTNNAC 5 cut(s) 737, 910, 1167, 1258, 1411
Hpy99I CGWCG 7 cut(s) 80, 88, 101, 154, 157, 196, 2028
HpyAV CCTTC 9 cut(s) 127, 646, 1003, 1088, 1272, 1390, 1515, 1669, 2060
HpyCH4III ACNGT 4 cut(s) 1052, 1433, 1541, 2028
HpyCH4IV ACGT 2 cut(s) 152, 1169
HpyCH4V TGCA 8 cut(s) 39, 343, 910, 1313, 1328, 1559, 1730, 1785
HpyF10VI GCNNNNNNNGC 7 cut(s) 184, 288, 589, 1445, 1694, 1973, 1982
HpyF3I CTNAG 5 cut(s) 1075, 1295, 1344, 1353, 1811
HpySE526I ACGT 2 cut(s) 152, 1169
Hsp92I GRCGYC 2 cut(s) 152, 216
Hsp92II CATG 8 cut(s) 583, 701, 806, 1073, 1576, 1592, 1994, 2036
HspAI GCGC 4 cut(s) 216, 228, 282, 1931
KasI GGCGCC 1 cut(s) 215
KpnI GGTACC 1 cut(s) 2032
KroI GCCGGC 2 cut(s) 284, 785
KroNI GCCGGC 2 cut(s) 286, 787
Ksp22I TGATCA 1 cut(s) 448
KspAI GTTAAC 1 cut(s) 1258
LguI GCTCTTC 2 cut(s) 168, 556
LmnI GCTCC 3 cut(s) 540, 877, 1694
Lsp1109I GCAGC 5 cut(s) 275, 355, 705, 1976, 1983
LweI GCATC 5 cut(s) 859, 1032, 1300, 1624, 1717
MaeI CTAG 4 cut(s) 107, 672, 1736, 2000
MaeII ACGT 2 cut(s) 152, 1169
MaeIII GTNAC 5 cut(s) 5, 1209, 1339, 1535, 1678
MbiI CCGCTC 3 cut(s) 94, 247, 566
MflI RGATCY 1 cut(s) 1924
MhlI GDGCHC 5 cut(s) 270, 317, 443, 775, 912
MluCI AATT 8 cut(s) 491, 685, 951, 957, 973, 1105, 1219, 1895
Mly113I GGCGCC 1 cut(s) 216
MlyI GAGTC 5 cut(s) 131, 394, 1147, 1743, 1845
MmeI TCCRAC 4 cut(s) 66, 1113, 1125, 2043
Mph1103I ATGCAT 1 cut(s) 1315
MroNI GCCGGC 2 cut(s) 284, 785
MroXI GAANNNNTTC 3 cut(s) 323, 505, 554
MseI TTAA 9 cut(s) 614, 684, 858, 971, 1223, 1257, 1275, 1544, 1832
MslI CAYNNNNRTG 4 cut(s) 629, 1589, 2037, 2051
MspR9I CCNGG 2 cut(s) 368, 379
Mva1269I GAATGC 1 cut(s) 1985
MvaI CCWGG 1 cut(s) 368
MwoI GCNNNNNNNGC 7 cut(s) 184, 288, 589, 1445, 1694, 1973, 1982
NaeI GCCGGC 2 cut(s) 286, 787
NarI GGCGCC 1 cut(s) 216
NciI CCSGG 1 cut(s) 379
NcoI CCATGG 1 cut(s) 1572
NgoMIV GCCGGC 2 cut(s) 284, 785
NlaIII CATG 8 cut(s) 583, 701, 806, 1073, 1576, 1592, 1994, 2036
NlaIV GGNNCC 8 cut(s) 90, 217, 351, 415, 441, 468, 577, 2030
NmuCI GTSAC 2 cut(s) 1339, 1678
NsbI TGCGCA 1 cut(s) 1932
NsiI ATGCAT 1 cut(s) 1315
NspI RCATGY 2 cut(s) 1073, 1592
NspV TTCGAA 1 cut(s) 948
OliI CACNNNNGTG 1 cut(s) 1589
PaeR7I CTCGAG 2 cut(s) 496, 1683
PaqCI CACCTGC 1 cut(s) 1790
PciI ACATGT 1 cut(s) 1588
PciSI GCTCTTC 2 cut(s) 168, 556
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 248
PctI GAATGC 1 cut(s) 1985
PdiI GCCGGC 2 cut(s) 286, 787
PdmI GAANNNNTTC 3 cut(s) 323, 505, 554
PfeI GAWTC 6 cut(s) 638, 644, 760, 1036, 1348, 1514
PflFI GACNNNGTC 2 cut(s) 154, 1052
PkrI GCNGC 7 cut(s) 246, 290, 301, 345, 695, 1966, 1998
Ple19I CGATCG 3 cut(s) 295, 393, 474
PleI GAGTC 5 cut(s) 131, 393, 1147, 1743, 1845
PluTI GGCGCC 1 cut(s) 219
PpsI GAGTC 5 cut(s) 131, 393, 1147, 1743, 1845
PpuMI RGGWCCY 1 cut(s) 413
PscI ACATGT 1 cut(s) 1588
PshBI ATTAAT 1 cut(s) 684
Psp124BI GAGCTC 2 cut(s) 270, 317
Psp5II RGGWCCY 1 cut(s) 413
Psp6I CCWGG 1 cut(s) 366
PspFI CCCAGC 2 cut(s) 57, 828
PspGI CCWGG 1 cut(s) 366
PspN4I GGNNCC 8 cut(s) 90, 217, 351, 415, 441, 468, 577, 2030
PspOMI GGGCCC 1 cut(s) 439
PspPI GGNCC 8 cut(s) 88, 103, 132, 413, 439, 440, 467, 863
PspPPI RGGWCCY 1 cut(s) 413
PspXI VCTCGAGB 1 cut(s) 1683
PstI CTGCAG 2 cut(s) 345, 1330
PsuI RGATCY 1 cut(s) 1924
PsyI GACNNNGTC 2 cut(s) 154, 1052
PvuI CGATCG 3 cut(s) 295, 393, 474
RsaI GTAC 4 cut(s) 278, 1605, 1862, 2030
RsaNI GTAC 4 cut(s) 277, 1604, 1861, 2029
RseI CAYNNNNRTG 4 cut(s) 629, 1589, 2037, 2051
SacI GAGCTC 2 cut(s) 270, 317
SapI GCTCTTC 2 cut(s) 168, 556
SaqAI TTAA 9 cut(s) 614, 684, 858, 971, 1223, 1257, 1275, 1544, 1832
SatI GCNGC 7 cut(s) 245, 289, 300, 344, 694, 1965, 1997
Sau96I GGNCC 8 cut(s) 88, 103, 132, 413, 439, 440, 467, 863
ScaI AGTACT 1 cut(s) 1862
SchI GAGTC 5 cut(s) 131, 394, 1147, 1743, 1845
ScrFI CCNGG 2 cut(s) 368, 379
SduI GDGCHC 5 cut(s) 270, 317, 443, 775, 912
SfaNI GCATC 5 cut(s) 859, 1032, 1300, 1624, 1717
SfcI CTRYAG 2 cut(s) 341, 1326
SfoI GGCGCC 1 cut(s) 217
Sfr274I CTCGAG 2 cut(s) 496, 1683
SfuI TTCGAA 1 cut(s) 948
SinI GGWCC 3 cut(s) 88, 413, 467
SlaI CTCGAG 2 cut(s) 496, 1683
SmiMI CAYNNNNRTG 4 cut(s) 629, 1589, 2037, 2051
SmlI CTYRAG 3 cut(s) 496, 774, 1683
SmoI CTYRAG 3 cut(s) 496, 774, 1683
Sse9I AATT 8 cut(s) 491, 685, 951, 957, 973, 1105, 1219, 1895
SsiI CCGC 8 cut(s) 42, 92, 185, 245, 299, 566, 1439, 1474
SspDI GGCGCC 1 cut(s) 215
SspI AATATT 2 cut(s) 1550, 1836
SspMI CTAG 4 cut(s) 107, 672, 1736, 2000
SstI GAGCTC 2 cut(s) 270, 317
StyD4I CCNGG 2 cut(s) 366, 377
StyI CCWWGG 2 cut(s) 106, 1572
TaaI ACNGT 4 cut(s) 1052, 1433, 1541, 2028
TaiI ACGT 2 cut(s) 155, 1172
TasI AATT 8 cut(s) 491, 685, 951, 957, 973, 1105, 1219, 1895
TatI WGTACW 2 cut(s) 1603, 1860
TauI GCSGC 2 cut(s) 247, 302
TfiI GAWTC 6 cut(s) 638, 644, 760, 1036, 1348, 1514
Tru1I TTAA 9 cut(s) 614, 684, 858, 971, 1223, 1257, 1275, 1544, 1832
Tru9I TTAA 9 cut(s) 614, 684, 858, 971, 1223, 1257, 1275, 1544, 1832
TscAI CASTG 6 cut(s) 636, 1239, 1299, 1330, 1737, 2094
TseFI GTSAC 2 cut(s) 1339, 1678
TseI GCWGC 5 cut(s) 288, 343, 693, 1964, 1996
Tsp45I GTSAC 2 cut(s) 1339, 1678
TspGWI ACGGA 3 cut(s) 64, 211, 1001
TspRI CASTG 6 cut(s) 636, 1239, 1299, 1330, 1737, 2094
Tth111I GACNNNGTC 2 cut(s) 154, 1052
VneI GTGCAC 1 cut(s) 908
VpaK11BI GGWCC 3 cut(s) 88, 413, 467
VspI ATTAAT 1 cut(s) 684
XapI RAATTY 3 cut(s) 491, 973, 1219
XceI RCATGY 2 cut(s) 1073, 1592
XhoI CTCGAG 2 cut(s) 496, 1683
XmaJI CCTAGG 1 cut(s) 106
XmnI GAANNNNTTC 3 cut(s) 323, 505, 554
XspI CTAG 4 cut(s) 107, 672, 1736, 2000
ZraI GACGTC 1 cut(s) 153
ZrmI AGTACT 1 cut(s) 1862
Zsp2I ATGCAT 1 cut(s) 1315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.