Rh3BG151400

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0004926
Physical Location & Seq
Forward (+)
12846649 .. 12849782
3134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG151400.1

Sequence Viewer

Length: 1680 bp
ATGGGCAACGCCATCGCAAAGGTCTCTCACTGCTGCTCTGTCGCCGGCGATGTCTCCAGGCGCCACGACGTCGACGTCGTCTCCCCCGACGAGGGTCTCGGCCACTCCTTCTACTACATTCGCCGCCCAGAAATAATCTCCCCCACGACCTCGTTTGAGGCTTCCTCTGAGGAGTTCCCCAACCCGGCTACCACCACGTTCCGGTCAATCTCCGGCGCCTCCGTCAGCGCCAACACGTTCACGCCGCCGTCTACTACACTATATGATCTCTACCCCGACAGCTCCGGTGGTCTCGATCGTGCTGCTAGGTTTGAGAGCTCTACTTCGTTCGCTTCGATTCCTCTGCAGCCGGTTCCTCGGAACCCGGGACTTCCGGGGTCCGGTCCTGCTGAAAGAGGCTTCTTGTCCGGTCCGATCGAGCGGGGGTTCATGTCCGGTCCGATTGACCGGTTGGTGTGTTCGGGTCCGATTGAGAAGCAGTCCGAGAAGATGGAGAGAAGCCTCTCTTTTGGGCTTGAGAAAAAGCCCAAGAGAAGGAATTTGATGAGGATGATAAGAAGAGCGATTTCGAAGAGTATGAATCGGCGGCACAAGTCGGCTGTGACGCCGAAAGAAACGGAGTCGGAGAAAATCGGCGAGGAGTTGAATCCGAGTAGTACTAACTTGAGCAGCCATGCGAGTTTGAGTGGAAATGAGAATGGGATTGGGGATTTCTCAATGGAAAGTCAGAATCTTCAGTGGGCTCAAGGGAAGGCGGGTGAGGATCGAGTTCATGTTGTGGTTTCTGATGAGCATGGTTGGGTGTTCGTGGGGATTTATGATGGGTTTAATGGCCCTGATGCTCCTGATTATTTGTTGTCTAATCTTTACTCTGCTGTGCATGAGGAGCTCAAGGGATTGCTGTGGAGTGACATGTTGGAAATCGAATCCATGGATTTGAAATCATCGGAGTCGAATGTGGTTTCGTGTTCTTTTAGTGAGGTTGGCTGCATTGATGGAGTTGATAAGGAGAATTGTACAGTGGAGAATGTGTTGTCAAATAGAAAACAGAGCAGGCATTTGAGGAATGAAAGTAATGAGATGGATAATAAGTGGGAGGAGAGTCAGAGGAGATGGAAGTGTGAATGGGATAGAGAAAGGCCAGAGCTTGATAGGTTAACTAAGGAGAACTTGAAGCTTAAGGTGTCAAATGGTGTGGCAGCTGCGAACCCCTCGGATGTTCTCAAGGCTCTTTCGGAGGCATTAAGAAAAACTGAAGAGGCCTATTTGGATGTTGCGGATAAGATGGTTACGGAAAATCCTGAGCTGGCTCTGATGGGGTCTTGTGTGCTGGTGATGTTGATGAAGGGAGATGATGTTTATCTGATGAATGTTGGGGATAGTCGTGCGGTTTTAGCTCAGAAAGCCGATCCCAAGATCAAGCGAAACTTGGAACGGATTAATGAGGAAGCTTTATGCGAACGTCGATCATCTAGTGCTGATGAGCTTTACAGTTTAAACAATTTGACTTCGGTTCAGCTGACAATGGATCACAGCACAAGTGTGAAAGAGGAAGTTCGAAGAATAAGGAATGAACATCCAGATGATGCTACTGCAGTGATGAATAAGAGAGTGAAGGGTTATTTGAAGGTCACTCGGGCATTTGGTGCCGGCTTTCTAAAACAGGTACTTTTAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0071944 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

559

Amino Acids

62.0

Weight (kDa)

5.66

Isoelectric Point (pI)

52.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 403 - 555 1.7e-15 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
AatII GACGTC 2 cut(s) 72, 78
AccB1I GGYRCC 3 cut(s) 60, 215, 1646
AccBSI CCGCTC 1 cut(s) 421
AccI GTMKAC 2 cut(s) 72, 251
AciI CCGC 7 cut(s) 124, 245, 421, 586, 755, 1277, 1388
AclWI GGATC 3 cut(s) 771, 1403, 1536
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 1 cut(s) 538
AcuI CTGAAG 2 cut(s) 719, 1275
AcyI GRCGYC 5 cut(s) 61, 69, 75, 216, 605
AfaI GTAC 3 cut(s) 658, 1018, 1668
AfiI CCNNNNNNNGG 6 cut(s) 91, 92, 184, 201, 380, 534
AflII CTTAAG 1 cut(s) 1178
AflIII ACRYGT 2 cut(s) 234, 912
AgeI ACCGGT 1 cut(s) 447
AgsI TTSAA 4 cut(s) 646, 940, 1174, 1627
AjnI CCWGG 1 cut(s) 56
AleI CACNNNNGTG 1 cut(s) 1541
Alw21I GWGCWC 2 cut(s) 320, 891
Alw26I GTCTC 5 cut(s) 28, 58, 85, 101, 296
AlwI GGATC 3 cut(s) 771, 1403, 1536
Ama87I CYCGRG 2 cut(s) 364, 1635
AoxI GGCC 4 cut(s) 100, 832, 1139, 1260
ApeKI GCWGC 7 cut(s) 33, 302, 346, 669, 987, 1199, 1202
ApoI RAATTY 1 cut(s) 538
AseI ATTAAT 1 cut(s) 1440
AsiGI ACCGGT 1 cut(s) 447
AspLEI GCGC 3 cut(s) 63, 218, 230
AspS9I GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
AsuC2I CCSGG 4 cut(s) 185, 365, 366, 375
AsuHPI GGTGA 2 cut(s) 770, 1345
AsuII TTCGAA 2 cut(s) 569, 1558
AvaI CYCGRG 2 cut(s) 364, 1635
AvaII GGWCC 5 cut(s) 378, 383, 410, 437, 464
BanI GGYRCC 3 cut(s) 60, 215, 1646
BanII GRGCYC 3 cut(s) 320, 745, 891
Bbv12I GWGCWC 2 cut(s) 320, 891
BbvI GCAGC 7 cut(s) 20, 289, 358, 681, 974, 1189, 1211
BccI CCATC 8 cut(s) 20, 484, 815, 989, 1075, 1107, 1279, 1309
BceAI ACGGC 1 cut(s) 232
BcgI CGANNNNNNTGC 5 cut(s) 29, 284, 318, 325, 359
BciT130I CCWGG 1 cut(s) 58
BcnI CCSGG 4 cut(s) 185, 365, 366, 375
BcoDI GTCTC 5 cut(s) 28, 58, 85, 101, 296
BfaI CTAG 2 cut(s) 306, 1473
BfmI CTRYAG 2 cut(s) 344, 1593
BfoI RGCGCY 3 cut(s) 64, 219, 231
BfrI CTTAAG 1 cut(s) 1178
BmcAI AGTACT 1 cut(s) 658
Bme1390I CCNGG 5 cut(s) 58, 185, 365, 366, 375
Bme18I GGWCC 5 cut(s) 378, 383, 410, 437, 464
BmeT110I CYCGRG 2 cut(s) 364, 1635
BmgT120I GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
BmiI GGNNCC 7 cut(s) 62, 217, 354, 362, 379, 465, 1648
BmrFI CCNGG 5 cut(s) 58, 185, 365, 366, 375
BmsI GCATC 2 cut(s) 829, 1576
BoxI GACNNNNGTC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BpmI CTGGAG 1 cut(s) 40
Bpu10I CCTNAGC 1 cut(s) 1302
Bpu14I TTCGAA 2 cut(s) 569, 1558
BpuEI CTTGAG 5 cut(s) 536, 685, 729, 875, 1208
BpuMI CCSGG 4 cut(s) 185, 365, 366, 375
BsaBI GATNNNNATC 1 cut(s) 1359
BsaHI GRCGYC 5 cut(s) 61, 69, 75, 216, 605
BsaI GGTCTC 3 cut(s) 28, 101, 296
BsaJI CCNNGG 5 cut(s) 356, 364, 374, 930, 1212
BsaWI WCCGGW 6 cut(s) 201, 284, 380, 407, 434, 447
BsaXI ACNNNNNCTCC 6 cut(s) 65, 95, 1016, 1046, 1341, 1371
Bsc4I CCNNNNNNNGG 6 cut(s) 91, 92, 184, 201, 380, 534
Bse118I RCCGGY 4 cut(s) 44, 349, 447, 1649
Bse8I GATNNNNATC 1 cut(s) 1359
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 5 cut(s) 356, 364, 374, 930, 1212
BseGI GGATG 4 cut(s) 555, 1222, 1276, 1576
BseJI GATNNNNATC 1 cut(s) 1359
BseLI CCNNNNNNNGG 6 cut(s) 91, 92, 184, 201, 380, 534
BseMII CTCAG 3 cut(s) 159, 1293, 1412
BseRI GAGGAG 5 cut(s) 185, 653, 899, 1112, 1123
BseXI GCAGC 7 cut(s) 20, 289, 358, 681, 974, 1189, 1211
Bsh1285I CGRYCG 2 cut(s) 298, 417
BshFI GGCC 4 cut(s) 102, 834, 1141, 1262
BshNI GGYRCC 3 cut(s) 60, 215, 1646
BshTI ACCGGT 1 cut(s) 447
BsiEI CGRYCG 2 cut(s) 298, 417
BsiHKAI GWGCWC 2 cut(s) 320, 891
BsiHKCI CYCGRG 2 cut(s) 364, 1635
BslFI GGGAC 1 cut(s) 381
BslI CCNNNNNNNGG 6 cut(s) 91, 92, 184, 201, 380, 534
BsmAI GTCTC 5 cut(s) 28, 58, 85, 101, 296
BsmBI CGTCTC 1 cut(s) 85
BsmFI GGGAC 1 cut(s) 381
BsnI GGCC 4 cut(s) 102, 834, 1141, 1262
Bso31I GGTCTC 3 cut(s) 28, 101, 296
BsoBI CYCGRG 2 cut(s) 364, 1635
Bsp119I TTCGAA 2 cut(s) 569, 1558
Bsp1286I GDGCHC 3 cut(s) 320, 745, 891
Bsp1407I TGTACA 1 cut(s) 1016
Bsp143I GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
Bsp19I CCATGG 1 cut(s) 930
BspACI CCGC 7 cut(s) 124, 245, 421, 586, 755, 1277, 1388
BspANI GGCC 4 cut(s) 102, 834, 1141, 1262
BspCNI CTCAG 3 cut(s) 160, 1294, 1411
BspLI GGNNCC 7 cut(s) 62, 217, 354, 362, 379, 465, 1648
BspMAI CTGCAG 2 cut(s) 348, 1597
BspPI GGATC 3 cut(s) 771, 1403, 1536
BspQI GCTCTTC 1 cut(s) 553
BspT104I TTCGAA 2 cut(s) 569, 1558
BspT107I GGYRCC 3 cut(s) 60, 215, 1646
BspTI CTTAAG 1 cut(s) 1178
BspTNI GGTCTC 3 cut(s) 28, 101, 296
BsrBI CCGCTC 1 cut(s) 421
BsrFI RCCGGY 4 cut(s) 44, 349, 447, 1649
BsrGI TGTACA 1 cut(s) 1016
BssAI RCCGGY 4 cut(s) 44, 349, 447, 1649
BssECI CCNNGG 5 cut(s) 356, 364, 374, 930, 1212
BssMI GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
BssNI GRCGYC 5 cut(s) 61, 69, 75, 216, 605
BssT1I CCWWGG 1 cut(s) 930
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 2 cut(s) 1021, 1493
Bst6I CTCTTC 3 cut(s) 553, 566, 1251
BstACI GRCGYC 5 cut(s) 61, 69, 75, 216, 605
BstAFI CTTAAG 1 cut(s) 1178
BstAPI GCANNNNNTGC 1 cut(s) 1646
BstAUI TGTACA 1 cut(s) 1016
BstBI TTCGAA 2 cut(s) 569, 1558
BstC8I GCNNGC 4 cut(s) 46, 1055, 1308, 1651
BstDEI CTNAG 4 cut(s) 168, 1161, 1302, 1398
BstDSI CCRYGG 1 cut(s) 930
BstF5I GGATG 4 cut(s) 555, 1222, 1276, 1576
BstH2I RGCGCY 3 cut(s) 64, 219, 231
BstHHI GCGC 3 cut(s) 63, 218, 230
BstKTI GATC 8 cut(s) 268, 298, 417, 766, 1411, 1419, 1469, 1531
BstMAI GTCTC 5 cut(s) 28, 58, 85, 101, 296
BstMBI GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
BstMCI CGRYCG 2 cut(s) 298, 417
BstMWI GCNNNNNNNGC 4 cut(s) 886, 1394, 1403, 1646
BstNI CCWGG 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 916
BstPAI GACNNNNGTC 1 cut(s) 93
BstSCI CCNGG 5 cut(s) 56, 183, 363, 364, 373
BstSFI CTRYAG 2 cut(s) 344, 1593
BstV1I GCAGC 7 cut(s) 20, 289, 358, 681, 974, 1189, 1211
BsuRI GGCC 4 cut(s) 102, 834, 1141, 1262
BtgI CCRYGG 1 cut(s) 930
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 4 cut(s) 555, 1222, 1276, 1576
BtsI GCAGTG 2 cut(s) 28, 1602
BtsIMutI CAGTG 4 cut(s) 28, 743, 1026, 1602
Cac8I GCNNGC 4 cut(s) 46, 1055, 1308, 1651
CfoI GCGC 3 cut(s) 63, 218, 230
Cfr10I RCCGGY 4 cut(s) 44, 349, 447, 1649
Cfr13I GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
Cfr9I CCCGGG 1 cut(s) 364
CpoI CGGWCCG 2 cut(s) 410, 437
CseI GACGC 1 cut(s) 613
Csp6I GTAC 3 cut(s) 657, 1017, 1667
CspAI ACCGGT 1 cut(s) 447
CspCI CAANNNNNGTGG 2 cut(s) 1176, 1211
CspI CGGWCCG 2 cut(s) 410, 437
CviAII CATG 7 cut(s) 430, 674, 773, 794, 881, 913, 931
CviQI GTAC 3 cut(s) 657, 1017, 1667
DdeI CTNAG 4 cut(s) 168, 1161, 1302, 1398
DinI GGCGCC 2 cut(s) 62, 217
DpnI GATC 8 cut(s) 267, 297, 416, 765, 1410, 1418, 1468, 1530
DpnII GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
DraI TTTAAA 1 cut(s) 1497
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 100
Eam1104I CTCTTC 3 cut(s) 553, 566, 1251
EarI CTCTTC 3 cut(s) 553, 566, 1251
Ecl136II GAGCTC 2 cut(s) 318, 889
Eco130I CCWWGG 1 cut(s) 930
Eco147I AGGCCT 1 cut(s) 1262
Eco24I GRGCYC 3 cut(s) 320, 745, 891
Eco31I GGTCTC 3 cut(s) 28, 101, 296
Eco47I GGWCC 5 cut(s) 378, 383, 410, 437, 464
Eco53kI GAGCTC 2 cut(s) 318, 889
Eco57I CTGAAG 2 cut(s) 719, 1275
Eco88I CYCGRG 2 cut(s) 364, 1635
EcoICRI GAGCTC 2 cut(s) 318, 889
EcoRII CCWGG 1 cut(s) 56
EcoT14I CCWWGG 1 cut(s) 930
EcoT38I GRGCYC 3 cut(s) 320, 745, 891
EgeI GGCGCC 2 cut(s) 62, 217
EheI GGCGCC 2 cut(s) 62, 217
ErhI CCWWGG 1 cut(s) 930
Esp3I CGTCTC 1 cut(s) 85
FaeI CATG 7 cut(s) 433, 677, 776, 797, 884, 916, 934
FalI AAGNNNNNCTT 6 cut(s) 490, 522, 1154, 1186, 1412, 1444
FaqI GGGAC 1 cut(s) 381
FatI CATG 7 cut(s) 429, 673, 772, 793, 880, 912, 930
FauI CCCGC 2 cut(s) 414, 748
FblI GTMKAC 2 cut(s) 72, 251
FokI GGATG 4 cut(s) 562, 1229, 1283, 1563
FriOI GRGCYC 3 cut(s) 320, 745, 891
FspBI CTAG 2 cut(s) 306, 1473
GlaI GCGC 3 cut(s) 62, 217, 229
GsuI CTGGAG 1 cut(s) 40
HaeII RGCGCY 3 cut(s) 64, 219, 231
HaeIII GGCC 4 cut(s) 102, 834, 1141, 1262
HgaI GACGC 1 cut(s) 613
HhaI GCGC 3 cut(s) 63, 218, 230
Hin1I GRCGYC 5 cut(s) 61, 69, 75, 216, 605
Hin1II CATG 7 cut(s) 433, 677, 776, 797, 884, 916, 934
Hin6I GCGC 3 cut(s) 61, 216, 228
HinP1I GCGC 3 cut(s) 61, 216, 228
HincII GTYRAC 2 cut(s) 73, 1158
HindII GTYRAC 2 cut(s) 73, 1158
HindIII AAGCTT 2 cut(s) 1175, 1449
HinfI GANTC 8 cut(s) 337, 580, 620, 646, 730, 926, 950, 1102
HpaI GTTAAC 1 cut(s) 1158
HphI GGTGA 2 cut(s) 770, 1345
Hpy166II GTNNAC 4 cut(s) 73, 240, 252, 1158
Hpy188III TCNNGA 4 cut(s) 293, 845, 1301, 1580
Hpy8I GTNNAC 4 cut(s) 73, 240, 252, 1158
Hpy99I CGWCG 6 cut(s) 71, 74, 77, 80, 92, 1467
HpyAV CCTTC 6 cut(s) 118, 528, 745, 1339, 1609, 1621
HpyCH4III ACNGT 2 cut(s) 1021, 1493
HpyCH4IV ACGT 5 cut(s) 69, 75, 197, 236, 1462
HpyCH4V TGCA 4 cut(s) 346, 880, 990, 1595
HpyF10VI GCNNNNNNNGC 4 cut(s) 886, 1394, 1403, 1646
HpyF3I CTNAG 4 cut(s) 168, 1161, 1302, 1398
HpySE526I ACGT 5 cut(s) 69, 75, 197, 236, 1462
Hsp92I GRCGYC 5 cut(s) 61, 69, 75, 216, 605
Hsp92II CATG 7 cut(s) 433, 677, 776, 797, 884, 916, 934
HspAI GCGC 3 cut(s) 61, 216, 228
KasI GGCGCC 2 cut(s) 60, 215
KroI GCCGGC 2 cut(s) 44, 1649
KroNI GCCGGC 2 cut(s) 46, 1651
KspAI GTTAAC 1 cut(s) 1158
Kzo9I GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
LguI GCTCTTC 1 cut(s) 553
LmnI GCTCC 3 cut(s) 287, 847, 886
Lsp1109I GCAGC 7 cut(s) 20, 289, 358, 681, 974, 1189, 1211
LweI GCATC 2 cut(s) 829, 1576
MaeI CTAG 2 cut(s) 306, 1473
MaeII ACGT 5 cut(s) 69, 75, 197, 236, 1462
MaeIII GTNAC 4 cut(s) 601, 908, 1288, 1630
MalI GATC 8 cut(s) 267, 297, 416, 765, 1410, 1418, 1468, 1530
MbiI CCGCTC 1 cut(s) 421
MboI GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
MboII GAAGA 6 cut(s) 499, 570, 583, 725, 1268, 1572
MhlI GDGCHC 3 cut(s) 320, 745, 891
MluCI AATT 3 cut(s) 538, 1012, 1501
Mly113I GGCGCC 2 cut(s) 61, 216
MlyI GAGTC 3 cut(s) 629, 959, 1111
MmeI TCCRAC 2 cut(s) 603, 897
MreI CGCCGGCG 1 cut(s) 44
MroNI GCCGGC 2 cut(s) 44, 1649
MseI TTAA 7 cut(s) 828, 1157, 1179, 1244, 1440, 1496, 1678
MslI CAYNNNNRTG 2 cut(s) 1541, 1581
MspA1I CMGCKG 2 cut(s) 1202, 1519
MspCI CTTAAG 1 cut(s) 1178
MspR9I CCNGG 5 cut(s) 58, 185, 365, 366, 375
MssI GTTTAAAC 1 cut(s) 1497
MvaI CCWGG 1 cut(s) 58
MwoI GCNNNNNNNGC 4 cut(s) 886, 1394, 1403, 1646
NaeI GCCGGC 2 cut(s) 46, 1651
NarI GGCGCC 2 cut(s) 61, 216
NciI CCSGG 4 cut(s) 185, 365, 366, 375
NcoI CCATGG 1 cut(s) 930
NdeII GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
NgoMIV GCCGGC 2 cut(s) 44, 1649
NlaIII CATG 7 cut(s) 433, 677, 776, 797, 884, 916, 934
NlaIV GGNNCC 7 cut(s) 62, 217, 354, 362, 379, 465, 1648
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 3 cut(s) 601, 908, 1630
NspI RCATGY 1 cut(s) 916
NspV TTCGAA 2 cut(s) 569, 1558
OliI CACNNNNGTG 1 cut(s) 1541
PceI AGGCCT 1 cut(s) 1262
PciI ACATGT 1 cut(s) 912
PciSI GCTCTTC 1 cut(s) 553
PcsI WCGNNNNNNNCGW 4 cut(s) 72, 75, 84, 332
PdiI GCCGGC 2 cut(s) 46, 1651
PfeI GAWTC 5 cut(s) 337, 580, 646, 730, 926
PflFI GACNNNGTC 1 cut(s) 77
PinAI ACCGGT 1 cut(s) 447
Ple19I CGATCG 2 cut(s) 298, 417
PleI GAGTC 3 cut(s) 628, 958, 1110
PluTI GGCGCC 2 cut(s) 64, 219
PmeI GTTTAAAC 1 cut(s) 1497
PpsI GAGTC 3 cut(s) 628, 958, 1110
PscI ACATGT 1 cut(s) 912
PshAI GACNNNNGTC 1 cut(s) 93
PshBI ATTAAT 1 cut(s) 1440
Psp124BI GAGCTC 2 cut(s) 320, 891
Psp6I CCWGG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 56
PspN4I GGNNCC 7 cut(s) 62, 217, 354, 362, 379, 465, 1648
PspPI GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
PstI CTGCAG 2 cut(s) 348, 1597
PsyI GACNNNGTC 1 cut(s) 77
PvuI CGATCG 2 cut(s) 298, 417
PvuII CAGCTG 2 cut(s) 1202, 1519
RsaI GTAC 3 cut(s) 658, 1018, 1668
RsaNI GTAC 3 cut(s) 657, 1017, 1667
RseI CAYNNNNRTG 2 cut(s) 1541, 1581
Rsr2I CGGWCCG 2 cut(s) 410, 437
RsrII CGGWCCG 2 cut(s) 410, 437
SacI GAGCTC 2 cut(s) 320, 891
SalI GTCGAC 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 553
SaqAI TTAA 7 cut(s) 828, 1157, 1179, 1244, 1440, 1496, 1678
Sau3AI GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
Sau96I GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
ScaI AGTACT 1 cut(s) 658
SchI GAGTC 3 cut(s) 629, 959, 1111
ScrFI CCNGG 5 cut(s) 58, 185, 365, 366, 375
SduI GDGCHC 3 cut(s) 320, 745, 891
SfaNI GCATC 2 cut(s) 829, 1576
SfcI CTRYAG 2 cut(s) 344, 1593
SfoI GGCGCC 2 cut(s) 62, 217
SfuI TTCGAA 2 cut(s) 569, 1558
SgrAI CRCCGGYG 1 cut(s) 44
SgrDI CGTCGACG 1 cut(s) 71
SinI GGWCC 5 cut(s) 378, 383, 410, 437, 464
SmaI CCCGGG 1 cut(s) 366
SmiMI CAYNNNNRTG 2 cut(s) 1541, 1581
SmlI CTYRAG 6 cut(s) 515, 664, 744, 890, 1178, 1223
SmoI CTYRAG 6 cut(s) 515, 664, 744, 890, 1178, 1223
Sse9I AATT 3 cut(s) 538, 1012, 1501
SseBI AGGCCT 1 cut(s) 1262
SsiI CCGC 7 cut(s) 124, 245, 421, 586, 755, 1277, 1388
SspDI GGCGCC 2 cut(s) 60, 215
SspMI CTAG 2 cut(s) 306, 1473
SstI GAGCTC 2 cut(s) 320, 891
StuI AGGCCT 1 cut(s) 1262
StyD4I CCNGG 5 cut(s) 56, 183, 363, 364, 373
StyI CCWWGG 1 cut(s) 930
TaaI ACNGT 2 cut(s) 1021, 1493
TaiI ACGT 5 cut(s) 72, 78, 200, 239, 1465
TasI AATT 3 cut(s) 538, 1012, 1501
TatI WGTACW 2 cut(s) 656, 1016
TauI GCSGC 3 cut(s) 126, 247, 589
TfiI GAWTC 5 cut(s) 337, 580, 646, 730, 926
Tru1I TTAA 7 cut(s) 828, 1157, 1179, 1244, 1440, 1496, 1678
Tru9I TTAA 7 cut(s) 828, 1157, 1179, 1244, 1440, 1496, 1678
TscAI CASTG 4 cut(s) 35, 743, 1026, 1602
TseFI GTSAC 3 cut(s) 601, 908, 1630
TseI GCWGC 7 cut(s) 33, 302, 346, 669, 987, 1199, 1202
Tsp45I GTSAC 3 cut(s) 601, 908, 1630
TspDTI ATGAA 8 cut(s) 418, 593, 761, 1083, 1358, 1382, 1587, 1616
TspGWI ACGGA 4 cut(s) 211, 632, 1307, 1450
TspMI CCCGGG 1 cut(s) 364
TspRI CASTG 4 cut(s) 35, 743, 1026, 1602
Tth111I GACNNNGTC 1 cut(s) 77
Vha464I CTTAAG 1 cut(s) 1178
VpaK11BI GGWCC 5 cut(s) 378, 383, 410, 437, 464
VspI ATTAAT 1 cut(s) 1440
XapI RAATTY 1 cut(s) 538
XceI RCATGY 1 cut(s) 916
XmaI CCCGGG 1 cut(s) 364
XmiI GTMKAC 2 cut(s) 72, 251
XspI CTAG 2 cut(s) 306, 1473
ZraI GACGTC 2 cut(s) 70, 76
ZrmI AGTACT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.