Rroxscaffold_6G00403980

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004926
Physical Location & Seq
Reverse (-)
26421665 .. 26424000
2336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00403980.1

Sequence Viewer

Length: 1182 bp
ATGAATCGGCGGCACAAGTCGGCTGTGACGCCGAAAGAAACGGAGTGGGAGAAAATCGGCGAGGAGTTGAATCCGAGTAGTAATCTTCAGTGGGCTCAAGGGAAGGTGGGTGAGGATCGAGTTCATGTTGTGGTTTCTGATGAGCATGGTTGGGTGTTTGTGGGGATTTATGATGGGTTTAATGGTCCTGATGCTCCTGATTATTTGTTGTCTAATCTTTACTCTGCTGTGCATGAGGAGCTCAAGGGATTGCTGTGGAGTGACATGTTGGAAATCGAATCCACGGAATTGAAATCATCGGATTCGAATGTGGTTTCGTGTTCTTTTAGTGAGAGCAGGCATTTGAGGAATGAAAGTAATGAGATGGATAATAAGTGGGAGGAGAGTCAGAGGAGATGGAAGTGTGAATGGGATAGAGAAAGGCCAGAGCTTGATAGGAAAACTAAGGAGAACTTGAAGCTTAAGGTGTCGAATGGTGTGGCAGCTGCAAACCCCTCGGATGTTCTCAAGGCTCTTTTGGAGGCGTTAAGAAAAACTGAGGGGGCCTATTTGGATGTTGCGGATAAGATGGTTACCGAAAATCCTGAGTTGGCTCTGATGGGGTCTTGTGTGCTGGTGATGTTGATGAAGGGAGATGATGTTTACTTGATGAATGTTGGGGATAGTCGTGCGGTTTTAGCTCAGAAAGCCGATCCCAAGATCAAGCGAAACTTGGAACGGATTAATGAGGAAGCTTTATGCGAACGTCGATCATCTAGTGCTGATGAGCTTAACAGTTTAAACAATTTGACTTCGGTTCAGCTGACAATGGATCACAGCACAAGTGTGAAAGAGGAAGTTCGAAGAATGAGGAATGAACATCCAGATGATGCTACTGCAGTGATGAATAAGAGAGTGAAGGGTTATTTGAAGGTCACTCGGGCATTTTGTGCTGGCTTTCTAAAACAGTATTTCACCAATGAAGAAGCCGTCTCCGAAGTAGAGTTGTTCATCTCTTCCTTTCCTGATGGTGACCCTGCACAACATCTGATTAAAGAAGTGTTGTTTAGAGCAGCAAAGAAAGCTGGACTGGCCTTCCATGAACTGCTTGACATCCCACAAGGGGAACGTCGTCGGTACCATGATGACATTTCCATTATTATTATTTCACTCGAGGGAAGAATATGGCGTTCATCGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0071944 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

393

Amino Acids

44.74

Weight (kDa)

5.41

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 164 - 328 8.9e-14 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1116
AccB1I GGYRCC 1 cut(s) 1116
AciI CCGC 3 cut(s) 10, 560, 671
AclWI GGATC 3 cut(s) 123, 686, 819
AcuI CTGAAG 1 cut(s) 71
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 1 cut(s) 1118
AfiI CCNNNNNNNGG 1 cut(s) 1102
AflII CTTAAG 1 cut(s) 461
AflIII ACRYGT 1 cut(s) 264
AgsI TTSAA 4 cut(s) 70, 292, 457, 910
AleI CACNNNNGTG 1 cut(s) 824
AluBI AGCT 9 cut(s) 241, 430, 460, 485, 680, 734, 769, 802, 1064
AluI AGCT 9 cut(s) 241, 430, 460, 485, 680, 734, 769, 802, 1064
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 1 cut(s) 976
AlwI GGATC 3 cut(s) 123, 686, 819
Ama87I CYCGRG 2 cut(s) 918, 1151
AoxI GGCC 3 cut(s) 422, 543, 1071
ApeKI GCWGC 3 cut(s) 482, 485, 1052
AseI ATTAAT 1 cut(s) 723
Asp718I GGTACC 1 cut(s) 1116
AspS9I GGNCC 2 cut(s) 185, 543
AsuHPI GGTGA 4 cut(s) 122, 628, 946, 1022
AsuII TTCGAA 2 cut(s) 305, 841
AvaI CYCGRG 2 cut(s) 918, 1151
AvaII GGWCC 1 cut(s) 185
BanI GGYRCC 1 cut(s) 1116
BanII GRGCYC 2 cut(s) 97, 243
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 3 cut(s) 472, 494, 1064
BccI CCATC 6 cut(s) 167, 358, 390, 562, 592, 1001
BceAI ACGGC 1 cut(s) 953
BcgI CGANNNNNNTGC 2 cut(s) 477, 511
BcoDI GTCTC 1 cut(s) 976
BfaI CTAG 1 cut(s) 756
BfmI CTRYAG 1 cut(s) 876
BfrI CTTAAG 1 cut(s) 461
BisI GCNGC 4 cut(s) 11, 483, 486, 1053
BlsI GCNGC 4 cut(s) 12, 484, 487, 1054
Bme18I GGWCC 1 cut(s) 185
BmeT110I CYCGRG 2 cut(s) 918, 1151
BmgT120I GGNCC 2 cut(s) 185, 543
BmiI GGNNCC 2 cut(s) 544, 1118
BmsI GCATC 2 cut(s) 181, 859
Bpu14I TTCGAA 2 cut(s) 305, 841
BpuEI CTTGAG 3 cut(s) 81, 227, 491
BsaHI GRCGYC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 282, 495
BsaXI ACNNNNNCTCC 2 cut(s) 624, 654
Bsc4I CCNNNNNNNGG 1 cut(s) 1102
Bse1I ACTGG 1 cut(s) 1074
BseDI CCNNGG 2 cut(s) 282, 495
BseGI GGATG 4 cut(s) 505, 559, 859, 1092
BseLI CCNNNNNNNGG 1 cut(s) 1102
BseMII CTCAG 3 cut(s) 528, 576, 695
BseNI ACTGG 1 cut(s) 1074
BseRI GAGGAG 4 cut(s) 77, 251, 395, 406
BseXI GCAGC 3 cut(s) 472, 494, 1064
BsgI GTGCAG 1 cut(s) 1002
BshFI GGCC 3 cut(s) 424, 545, 1073
BshNI GGYRCC 1 cut(s) 1116
BsiHKAI GWGCWC 1 cut(s) 243
BsiHKCI CYCGRG 2 cut(s) 918, 1151
BslI CCNNNNNNNGG 1 cut(s) 1102
BsmAI GTCTC 1 cut(s) 976
BsmBI CGTCTC 1 cut(s) 976
BsnI GGCC 3 cut(s) 424, 545, 1073
BsoBI CYCGRG 2 cut(s) 918, 1151
Bsp119I TTCGAA 2 cut(s) 305, 841
Bsp1286I GDGCHC 2 cut(s) 97, 243
Bsp143I GATC 5 cut(s) 115, 691, 699, 749, 811
BspACI CCGC 3 cut(s) 10, 560, 671
BspANI GGCC 3 cut(s) 424, 545, 1073
BspCNI CTCAG 3 cut(s) 529, 577, 694
BspLI GGNNCC 2 cut(s) 544, 1118
BspMAI CTGCAG 1 cut(s) 880
BspPI GGATC 3 cut(s) 123, 686, 819
BspT104I TTCGAA 2 cut(s) 305, 841
BspT107I GGYRCC 1 cut(s) 1116
BspTI CTTAAG 1 cut(s) 461
BsrI ACTGG 1 cut(s) 1074
BssECI CCNNGG 2 cut(s) 282, 495
BssMI GATC 5 cut(s) 115, 691, 699, 749, 811
BssNI GRCGYC 1 cut(s) 29
Bst4CI ACNGT 2 cut(s) 776, 948
Bst6I CTCTTC 1 cut(s) 1000
BstACI GRCGYC 1 cut(s) 29
BstAFI CTTAAG 1 cut(s) 461
BstAPI GCANNNNNTGC 1 cut(s) 929
BstBI TTCGAA 2 cut(s) 305, 841
BstC8I GCNNGC 2 cut(s) 338, 934
BstDEI CTNAG 4 cut(s) 444, 537, 585, 681
BstDSI CCRYGG 1 cut(s) 282
BstEII GGTNACC 2 cut(s) 571, 1010
BstF5I GGATG 4 cut(s) 505, 559, 859, 1092
BstKTI GATC 5 cut(s) 118, 694, 702, 752, 814
BstMAI GTCTC 1 cut(s) 976
BstMBI GATC 5 cut(s) 115, 691, 699, 749, 811
BstMWI GCNNNNNNNGC 7 cut(s) 238, 677, 686, 929, 1061, 1070, 1174
BstNSI RCATGY 1 cut(s) 268
BstPI GGTNACC 2 cut(s) 571, 1010
BstSFI CTRYAG 1 cut(s) 876
BstV1I GCAGC 3 cut(s) 472, 494, 1064
BsuRI GGCC 3 cut(s) 424, 545, 1073
BtgI CCRYGG 1 cut(s) 282
BtgZI GCGATG 1 cut(s) 1158
BtsCI GGATG 4 cut(s) 505, 559, 859, 1092
BtsI GCAGTG 1 cut(s) 885
BtsIMutI CAGTG 2 cut(s) 95, 885
Cac8I GCNNGC 2 cut(s) 338, 934
Cfr13I GGNCC 2 cut(s) 185, 543
CseI GACGC 1 cut(s) 37
Csp6I GTAC 1 cut(s) 1117
CviAII CATG 6 cut(s) 125, 146, 233, 265, 1079, 1121
CviQI GTAC 1 cut(s) 1117
DdeI CTNAG 4 cut(s) 444, 537, 585, 681
DpnI GATC 5 cut(s) 117, 693, 701, 751, 813
DpnII GATC 5 cut(s) 115, 691, 699, 749, 811
DraI TTTAAA 1 cut(s) 780
Eam1104I CTCTTC 1 cut(s) 1000
EarI CTCTTC 1 cut(s) 1000
Ecl136II GAGCTC 1 cut(s) 241
Eco24I GRGCYC 2 cut(s) 97, 243
Eco47I GGWCC 1 cut(s) 185
Eco53kI GAGCTC 1 cut(s) 241
Eco57I CTGAAG 1 cut(s) 71
Eco88I CYCGRG 2 cut(s) 918, 1151
Eco91I GGTNACC 2 cut(s) 571, 1010
EcoICRI GAGCTC 1 cut(s) 241
EcoO109I RGGNCCY 1 cut(s) 543
EcoO65I GGTNACC 2 cut(s) 571, 1010
EcoT38I GRGCYC 2 cut(s) 97, 243
Esp3I CGTCTC 1 cut(s) 976
FaeI CATG 6 cut(s) 128, 149, 236, 268, 1082, 1124
FaiI YATR 9 cut(s) 126, 147, 171, 234, 266, 739, 1080, 1122, 1165
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 695, 727
FatI CATG 6 cut(s) 124, 145, 232, 264, 1078, 1120
Fnu4HI GCNGC 4 cut(s) 11, 483, 486, 1053
FokI GGATG 4 cut(s) 512, 566, 846, 1079
FriOI GRGCYC 2 cut(s) 97, 243
Fsp4HI GCNGC 4 cut(s) 11, 483, 486, 1053
FspBI CTAG 1 cut(s) 756
GluI GCNGC 4 cut(s) 11, 483, 486, 1053
HaeIII GGCC 3 cut(s) 424, 545, 1073
HgaI GACGC 1 cut(s) 37
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 6 cut(s) 128, 149, 236, 268, 1082, 1124
HindIII AAGCTT 2 cut(s) 458, 732
HinfI GANTC 5 cut(s) 4, 70, 278, 302, 385
HphI GGTGA 4 cut(s) 122, 628, 946, 1022
Hpy166II GTNNAC 1 cut(s) 643
Hpy188I TCNGA 9 cut(s) 75, 139, 301, 390, 499, 597, 684, 976, 1029
Hpy188III TCNNGA 5 cut(s) 188, 197, 584, 863, 1004
Hpy8I GTNNAC 1 cut(s) 643
Hpy99I CGWCG 3 cut(s) 750, 1113, 1116
HpyAV CCTTC 5 cut(s) 97, 622, 892, 904, 1084
HpyCH4III ACNGT 2 cut(s) 776, 948
HpyCH4IV ACGT 2 cut(s) 745, 1108
HpyCH4V TGCA 4 cut(s) 232, 488, 878, 1019
HpyF10VI GCNNNNNNNGC 7 cut(s) 238, 677, 686, 929, 1061, 1070, 1174
HpyF3I CTNAG 4 cut(s) 444, 537, 585, 681
HpySE526I ACGT 2 cut(s) 745, 1108
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 6 cut(s) 128, 149, 236, 268, 1082, 1124
KpnI GGTACC 1 cut(s) 1120
Kzo9I GATC 5 cut(s) 115, 691, 699, 749, 811
LmnI GCTCC 2 cut(s) 199, 238
Lsp1109I GCAGC 3 cut(s) 472, 494, 1064
LweI GCATC 2 cut(s) 181, 859
MaeI CTAG 1 cut(s) 756
MaeII ACGT 2 cut(s) 745, 1108
MaeIII GTNAC 5 cut(s) 25, 260, 571, 913, 1010
MalI GATC 5 cut(s) 117, 693, 701, 751, 813
MboI GATC 5 cut(s) 115, 691, 699, 749, 811
MboII GAAGA 5 cut(s) 77, 855, 974, 987, 1170
MhlI GDGCHC 2 cut(s) 97, 243
MluCI AATT 2 cut(s) 287, 784
MlyI GAGTC 1 cut(s) 394
MmeI TCCRAC 1 cut(s) 249
MseI TTAA 7 cut(s) 180, 462, 527, 723, 771, 779, 1032
MslI CAYNNNNRTG 2 cut(s) 824, 864
MspA1I CMGCKG 2 cut(s) 485, 802
MspCI CTTAAG 1 cut(s) 461
MssI GTTTAAAC 1 cut(s) 780
MwoI GCNNNNNNNGC 7 cut(s) 238, 677, 686, 929, 1061, 1070, 1174
NdeII GATC 5 cut(s) 115, 691, 699, 749, 811
NlaIII CATG 6 cut(s) 128, 149, 236, 268, 1082, 1124
NlaIV GGNNCC 2 cut(s) 544, 1118
NmuCI GTSAC 4 cut(s) 25, 260, 913, 1010
NspI RCATGY 1 cut(s) 268
NspV TTCGAA 2 cut(s) 305, 841
OliI CACNNNNGTG 1 cut(s) 824
PaeR7I CTCGAG 1 cut(s) 1151
PciI ACATGT 1 cut(s) 264
PfeI GAWTC 4 cut(s) 4, 70, 278, 302
PkrI GCNGC 4 cut(s) 12, 484, 487, 1054
PleI GAGTC 1 cut(s) 393
PmeI GTTTAAAC 1 cut(s) 780
PpsI GAGTC 1 cut(s) 393
PscI ACATGT 1 cut(s) 264
PshBI ATTAAT 1 cut(s) 723
Psp124BI GAGCTC 1 cut(s) 243
PspEI GGTNACC 2 cut(s) 571, 1010
PspN4I GGNNCC 2 cut(s) 544, 1118
PspPI GGNCC 2 cut(s) 185, 543
PspXI VCTCGAGB 1 cut(s) 1151
PstI CTGCAG 1 cut(s) 880
PvuII CAGCTG 2 cut(s) 485, 802
RsaI GTAC 1 cut(s) 1118
RsaNI GTAC 1 cut(s) 1117
RseI CAYNNNNRTG 2 cut(s) 824, 864
SacI GAGCTC 1 cut(s) 243
SaqAI TTAA 7 cut(s) 180, 462, 527, 723, 771, 779, 1032
SatI GCNGC 4 cut(s) 11, 483, 486, 1053
Sau3AI GATC 5 cut(s) 115, 691, 699, 749, 811
Sau96I GGNCC 2 cut(s) 185, 543
SchI GAGTC 1 cut(s) 394
SduI GDGCHC 2 cut(s) 97, 243
SfaNI GCATC 2 cut(s) 181, 859
SfcI CTRYAG 1 cut(s) 876
Sfr274I CTCGAG 1 cut(s) 1151
SfuI TTCGAA 2 cut(s) 305, 841
SinI GGWCC 1 cut(s) 185
SlaI CTCGAG 1 cut(s) 1151
SmiMI CAYNNNNRTG 2 cut(s) 824, 864
SmlI CTYRAG 5 cut(s) 96, 242, 461, 506, 1151
SmoI CTYRAG 5 cut(s) 96, 242, 461, 506, 1151
Sse9I AATT 2 cut(s) 287, 784
SsiI CCGC 3 cut(s) 10, 560, 671
SspMI CTAG 1 cut(s) 756
SstI GAGCTC 1 cut(s) 243
TaaI ACNGT 2 cut(s) 776, 948
TaiI ACGT 2 cut(s) 748, 1111
TaqI TCGA 7 cut(s) 118, 276, 305, 470, 748, 841, 1152
TasI AATT 2 cut(s) 287, 784
TauI GCSGC 1 cut(s) 13
TfiI GAWTC 4 cut(s) 4, 70, 278, 302
Tru1I TTAA 7 cut(s) 180, 462, 527, 723, 771, 779, 1032
Tru9I TTAA 7 cut(s) 180, 462, 527, 723, 771, 779, 1032
TscAI CASTG 2 cut(s) 95, 885
TseFI GTSAC 4 cut(s) 25, 260, 913, 1010
TseI GCWGC 3 cut(s) 482, 485, 1052
Tsp45I GTSAC 4 cut(s) 25, 260, 913, 1010
TspGWI ACGGA 3 cut(s) 56, 299, 733
TspRI CASTG 2 cut(s) 95, 885
Vha464I CTTAAG 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 185
VspI ATTAAT 1 cut(s) 723
XceI RCATGY 1 cut(s) 268
XhoI CTCGAG 1 cut(s) 1151
XspI CTAG 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.