MD04G1128600.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004926
Physical Location & Seq
Reverse (-)
21460036 .. 21460801
766 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1128600.v1.1.491

Sequence Viewer

Length: 471 bp
ATGGTGACTGAGAATCCTGAGTTGGCTTTGATGGGTTCTTGTGTTTTGGTAATGCTGATGAAGGGAGATGATGTTTACTTGTTGAACGTTGGGGACAACAGGGTTGTTTTAGCTCGGAAGTCAGAACAAAACGTTCGGCGGAACTTGGATCGGATAAGTGAAGATGAGCCTTCATTTGATTCGTCAAATGCTGATGAAAGTTACAGTTTAAAACATTTGACTGCGCTTCAGCTCACCATGGATCATAGCACATATGTGGAGGAGAATAAAAAAGAGCATCCCGATGATGCATCTGCGATAATGAATGACAGAGTGAAAGGTTATTTGAAGGTCACTCGAGCATTTAGAGCTGGCTTTCTGAAACAGCCGAAGTGGAATGATGCACTGCTAGAGGTGTTTAGGCTCAACTATGTGATAGACATATATTGTGTGGCTGAGATACTGCCACTTGTGTTCAGATTAGAGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0071944 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

157

Amino Acids

17.87

Weight (kDa)

5.09

Isoelectric Point (pI)

34.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 10 - 122 4e-06 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 139
AclI AACGTT 2 cut(s) 87, 132
AclWI GGATC 2 cut(s) 156, 249
AcuI CTGAAG 1 cut(s) 212
AgsI TTSAA 2 cut(s) 85, 328
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AleI CACNNNNGTG 1 cut(s) 254
AluBI AGCT 3 cut(s) 113, 232, 350
AluI AGCT 3 cut(s) 113, 232, 350
AlwI GGATC 2 cut(s) 156, 249
Ama87I CYCGRG 1 cut(s) 336
AspLEI GCGC 1 cut(s) 226
AsuHPI GGTGA 2 cut(s) 16, 226
AvaI CYCGRG 1 cut(s) 336
BccI CCATC 1 cut(s) 25
BfaI CTAG 1 cut(s) 389
BmeT110I CYCGRG 1 cut(s) 336
BmsI GCATC 4 cut(s) 277, 286, 299, 370
BsaJI CCNNGG 1 cut(s) 237
BsaXI ACNNNNNCTCC 2 cut(s) 57, 87
BseDI CCNNGG 1 cut(s) 237
BseGI GGATG 1 cut(s) 277
BseMII CTCAG 2 cut(s) 9, 426
BseRI GAGGAG 1 cut(s) 275
BsiHKCI CYCGRG 1 cut(s) 336
BslFI GGGAC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 107
BsoBI CYCGRG 1 cut(s) 336
Bsp143I GATC 2 cut(s) 148, 241
Bsp19I CCATGG 1 cut(s) 237
BspACI CCGC 1 cut(s) 139
BspCNI CTCAG 2 cut(s) 10, 427
BspPI GGATC 2 cut(s) 156, 249
BssECI CCNNGG 1 cut(s) 237
BssMI GATC 2 cut(s) 148, 241
BssT1I CCWWGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 206
BstC8I GCNNGC 1 cut(s) 352
BstDEI CTNAG 3 cut(s) 9, 18, 435
BstDSI CCRYGG 1 cut(s) 237
BstF5I GGATG 1 cut(s) 277
BstHHI GCGC 1 cut(s) 226
BstKTI GATC 2 cut(s) 151, 244
BstMBI GATC 2 cut(s) 148, 241
BstMWI GCNNNNNNNGC 1 cut(s) 347
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 1 cut(s) 277
BtsI GCAGTG 1 cut(s) 383
BtsIMutI CAGTG 1 cut(s) 383
Cac8I GCNNGC 1 cut(s) 352
CfoI GCGC 1 cut(s) 226
CviAII CATG 1 cut(s) 238
CviJI RGCY 9 cut(s) 26, 113, 169, 232, 350, 354, 367, 403, 434
CviKI_1 RGCY 9 cut(s) 26, 113, 169, 232, 350, 354, 367, 403, 434
DdeI CTNAG 3 cut(s) 9, 18, 435
DpnI GATC 2 cut(s) 150, 243
DpnII GATC 2 cut(s) 148, 241
DraI TTTAAA 1 cut(s) 210
EciI GGCGGA 1 cut(s) 154
Eco130I CCWWGG 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 212
Eco88I CYCGRG 1 cut(s) 336
EcoT14I CCWWGG 1 cut(s) 237
EcoT22I ATGCAT 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 237
FaeI CATG 1 cut(s) 241
FaiI YATR 7 cut(s) 239, 246, 253, 255, 411, 422, 424
FaqI GGGAC 1 cut(s) 107
FatI CATG 1 cut(s) 237
FauNDI CATATG 1 cut(s) 253
FokI GGATG 1 cut(s) 264
FspBI CTAG 1 cut(s) 389
GlaI GCGC 1 cut(s) 225
HhaI GCGC 1 cut(s) 226
Hin1II CATG 1 cut(s) 241
Hin6I GCGC 1 cut(s) 224
HinP1I GCGC 1 cut(s) 224
HinfI GANTC 2 cut(s) 13, 179
HphI GGTGA 2 cut(s) 16, 226
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 5 cut(s) 117, 124, 153, 360, 458
Hpy188III TCNNGA 2 cut(s) 17, 281
Hpy8I GTNNAC 1 cut(s) 76
HpyAV CCTTC 3 cut(s) 55, 180, 322
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4IV ACGT 2 cut(s) 87, 132
HpyCH4V TGCA 2 cut(s) 290, 383
HpyF10VI GCNNNNNNNGC 1 cut(s) 347
HpyF3I CTNAG 3 cut(s) 9, 18, 435
HpySE526I ACGT 2 cut(s) 87, 132
Hsp92II CATG 1 cut(s) 241
HspAI GCGC 1 cut(s) 224
Kzo9I GATC 2 cut(s) 148, 241
LpnPI CCDG 3 cut(s) 30, 85, 336
LweI GCATC 4 cut(s) 277, 286, 299, 370
MaeI CTAG 1 cut(s) 389
MaeII ACGT 2 cut(s) 87, 132
MaeIII GTNAC 3 cut(s) 4, 200, 331
MalI GATC 2 cut(s) 150, 243
MboI GATC 2 cut(s) 148, 241
MboII GAAGA 1 cut(s) 173
MnlI CCTC 2 cut(s) 253, 385
Mph1103I ATGCAT 1 cut(s) 292
MseI TTAA 1 cut(s) 209
MslI CAYNNNNRTG 2 cut(s) 254, 282
MwoI GCNNNNNNNGC 1 cut(s) 347
NcoI CCATGG 1 cut(s) 237
NdeI CATATG 1 cut(s) 253
NdeII GATC 2 cut(s) 148, 241
NlaIII CATG 1 cut(s) 241
NmuCI GTSAC 2 cut(s) 4, 331
NsiI ATGCAT 1 cut(s) 292
OliI CACNNNNGTG 1 cut(s) 254
PaeR7I CTCGAG 1 cut(s) 336
PfeI GAWTC 2 cut(s) 13, 179
Psp1406I AACGTT 2 cut(s) 87, 132
PspXI VCTCGAGB 1 cut(s) 336
RseI CAYNNNNRTG 2 cut(s) 254, 282
SaqAI TTAA 1 cut(s) 209
Sau3AI GATC 2 cut(s) 148, 241
SetI ASST 8 cut(s) 90, 115, 135, 234, 322, 333, 352, 396
SfaNI GCATC 4 cut(s) 277, 286, 299, 370
Sfr274I CTCGAG 1 cut(s) 336
SlaI CTCGAG 1 cut(s) 336
SmiMI CAYNNNNRTG 2 cut(s) 254, 282
SmlI CTYRAG 1 cut(s) 336
SmoI CTYRAG 1 cut(s) 336
SsiI CCGC 1 cut(s) 139
SspMI CTAG 1 cut(s) 389
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 1 cut(s) 206
TaiI ACGT 2 cut(s) 90, 135
TaqI TCGA 1 cut(s) 337
TfiI GAWTC 2 cut(s) 13, 179
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TscAI CASTG 1 cut(s) 390
TseFI GTSAC 2 cut(s) 4, 331
Tsp45I GTSAC 2 cut(s) 4, 331
TspDTI ATGAA 4 cut(s) 74, 162, 210, 317
TspRI CASTG 1 cut(s) 390
XhoI CTCGAG 1 cut(s) 336
XspI CTAG 1 cut(s) 389
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.