Rroxscaffold_6G00414300

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004926
Physical Location & Seq
Reverse (-)
36623234 .. 36631064
7831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00414300.1

Sequence Viewer

Length: 1518 bp
ATGAATCGGCGCCACAAGTCGGCTGTGACGCCGAAAGAAATGGAGTTGGAGAAAATCGGCGAGGAGTTGAATCCGAGTAGTACTAACTTGAGCAGCCATGCGAGTTTGAGTGGAAATGAGAATGGGATTGGGGATTTCTCAATGGAAAGTCAGAATCTTCAGTGGGCTCAAGGGAAGGCGGGTGAGGATCGAGTTCATGTTGTGGTTTCTGATGAGCATGGTTGGGTGTTTGTGGGGATTTATGATGGGTTTAATGGCCCTGATGCTCCTGATTATTTGTTGTCGAATCTTTACTCTGCTGTACATGAGGAGCTCAAGGGATTGCTGTGGAGTGACATGTTGGAAATCGACTCCACGGATTTGAAATCATCGGATACGAATTTACTCGCCTGGCCATCTTCTTTGCGGAGGAGGACCCCACGTGATGATGTCGGTCTTGGCAGAGCTAGTGGGACTGGCTGCATTGACGGAGTTGAGAAGGAGAATTGTACAGTGGAGAATGTGTTGTCAAATAGAAAACAGAGCAGGCATTTGAGGAATGAAAGTAATGAGATGGATAATAAGTGGGAGGAGAGTCAGAGGAGATGGAAGTGTGAATGGGATAGAGAAAGGCCAGAGCTTGATAGGAAAACTAAGGAGAACTTGAAGCTTAAGGTGTCGAATGGTGTGGCAGCTGCAAACCCCTCGGATGTTCTCAAGGCTCTTTCGGAGGCATTAAGAAAAACTGAGGAGGCCTATTTGGATGTTGCGGATAAGATGGTCACCGAAAATCCTGAGTTGGCTCTGATGGGGTCTTGTGTGCTGGTGATGTTGATGAAGGGAGATGATGTTTACTTGATGAATGTTGGGGATAGTCGTGCGGTTTTAGCTCAGAAAGTCGATCCCAAAATCAAGCGAAGCTTGGAACGGATTAATGAGGAAGCTTTATGCGAACGTCGTTCATCTAGTGCTGATGAGCTTTACAGTTTAAACAATTTGACTTCGGTTCAGCTGACAATGGATCACAGCACAAGTGTGAAAGAGGAAGTTCGAAGAATAAGGAATGAACATCCAGATGATGCTACTGCAGTGATGAATAAGAGAGTGAAGGGTTATTTGAAGGTCACTCGGGCATTTGGTGCCGGCTTTCTAAAACAGCCAAAATGGAATGATGCACTGCTAGAGATGTTTAGACTCAATTATGTTGGGACTGCCCCCTACATCACATGTATACCATCAATGTATCACCACAAACTCAGACCAAGAGACAGACTCTTAATATTGTCCTCTGATGGACTCTATCAGTATTTCACCAATGAAGAAGCCGTCTCCGAAGTAGACTTGTTCATCTCTTCCTTTCCTGATGGTGACCCTGCACAACATCTGATTGAAGAAGTGTTGTTTCGAGCAGCAAAGAAAGCTGGACTGGCCTTCCATGAACTGCTTGACATCCCACAAGGGGAACGTCGTCGGTACCATGATGACATTTCCATTATTATTATTTCACTCGAGGGAAGAATATGGCGTTCATCGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0071944 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

505

Amino Acids

57.23

Weight (kDa)

5.54

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 225 - 446 3e-27 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1452
AccB1I GGYRCC 3 cut(s) 9, 1118, 1452
AccI GTMKAC 2 cut(s) 1210, 1317
AciI CCGC 4 cut(s) 179, 406, 749, 860
AclWI GGATC 3 cut(s) 195, 875, 1008
AcoI YGGCCR 1 cut(s) 392
AcsI RAATTY 1 cut(s) 379
AcuI CTGAAG 1 cut(s) 143
AcvI CACGTG 1 cut(s) 422
AcyI GRCGYC 2 cut(s) 10, 29
AfaI GTAC 4 cut(s) 82, 303, 490, 1454
AfiI CCNNNNNNNGG 2 cut(s) 19, 1438
AflII CTTAAG 1 cut(s) 650
AflIII ACRYGT 2 cut(s) 336, 1205
AgsI TTSAA 5 cut(s) 70, 364, 646, 1099, 1370
AjnI CCWGG 1 cut(s) 389
AleI CACNNNNGTG 1 cut(s) 1013
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 1239, 1312
AlwI GGATC 3 cut(s) 195, 875, 1008
Ama87I CYCGRG 2 cut(s) 1107, 1487
AoxI GGCC 5 cut(s) 256, 392, 611, 732, 1407
ApeKI GCWGC 5 cut(s) 93, 459, 671, 674, 1388
ApoI RAATTY 1 cut(s) 379
AseI ATTAAT 1 cut(s) 912
Asp718I GGTACC 1 cut(s) 1452
AspLEI GCGC 1 cut(s) 12
AspS9I GGNCC 2 cut(s) 257, 414
AsuHPI GGTGA 6 cut(s) 194, 754, 817, 1217, 1282, 1358
AsuII TTCGAA 1 cut(s) 1030
AvaI CYCGRG 2 cut(s) 1107, 1487
AvaII GGWCC 1 cut(s) 414
BalI TGGCCA 1 cut(s) 394
BanI GGYRCC 3 cut(s) 9, 1118, 1452
BanII GRGCYC 2 cut(s) 169, 315
BbrPI CACGTG 1 cut(s) 422
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 5 cut(s) 105, 446, 661, 683, 1400
BccI CCATC 9 cut(s) 239, 403, 547, 579, 751, 781, 1222, 1265, 1337
BceAI ACGGC 1 cut(s) 1289
BcgI CGANNNNNNTGC 2 cut(s) 666, 700
BciT130I CCWGG 1 cut(s) 391
BciVI GTATCC 1 cut(s) 367
BcoDI GTCTC 2 cut(s) 1239, 1312
BfaI CTAG 3 cut(s) 447, 945, 1160
BfmI CTRYAG 1 cut(s) 1065
BfoI RGCGCY 1 cut(s) 13
BfrI CTTAAG 1 cut(s) 650
BfuI GTATCC 1 cut(s) 367
BisI GCNGC 5 cut(s) 94, 460, 672, 675, 1389
BlsI GCNGC 5 cut(s) 95, 461, 673, 676, 1390
BmcAI AGTACT 1 cut(s) 82
Bme1390I CCNGG 1 cut(s) 391
Bme18I GGWCC 1 cut(s) 414
BmeT110I CYCGRG 2 cut(s) 1107, 1487
BmgT120I GGNCC 2 cut(s) 257, 414
BmiI GGNNCC 4 cut(s) 11, 416, 1120, 1454
BmrFI CCNGG 1 cut(s) 391
BmsI GCATC 3 cut(s) 253, 1048, 1141
BplI GAGNNNNNCTC 2 cut(s) 1236, 1268
Bpu14I TTCGAA 1 cut(s) 1030
BpuEI CTTGAG 4 cut(s) 109, 153, 299, 680
BsaAI YACGTR 1 cut(s) 422
BsaHI GRCGYC 2 cut(s) 10, 29
BsaJI CCNNGG 2 cut(s) 354, 684
BsaXI ACNNNNNCTCC 4 cut(s) 488, 518, 813, 843
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 1438
Bse118I RCCGGY 1 cut(s) 1121
Bse1I ACTGG 2 cut(s) 460, 1410
BseBI CCWGG 1 cut(s) 391
BseDI CCNNGG 2 cut(s) 354, 684
BseGI GGATG 4 cut(s) 694, 748, 1048, 1428
BseLI CCNNNNNNNGG 2 cut(s) 19, 1438
BseMII CTCAG 4 cut(s) 717, 765, 884, 1249
BseNI ACTGG 2 cut(s) 460, 1410
BseRI GAGGAG 6 cut(s) 77, 323, 424, 584, 595, 743
BseXI GCAGC 5 cut(s) 105, 446, 661, 683, 1400
BsgI GTGCAG 1 cut(s) 1338
BshFI GGCC 5 cut(s) 258, 394, 613, 734, 1409
BshNI GGYRCC 3 cut(s) 9, 1118, 1452
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 2 cut(s) 1107, 1487
BsiSI CCGG 1 cut(s) 1122
BslFI GGGAC 2 cut(s) 466, 1201
BslI CCNNNNNNNGG 2 cut(s) 19, 1438
BsmAI GTCTC 2 cut(s) 1239, 1312
BsmBI CGTCTC 1 cut(s) 1312
BsmFI GGGAC 2 cut(s) 466, 1201
BsnI GGCC 5 cut(s) 258, 394, 613, 734, 1409
BsoBI CYCGRG 2 cut(s) 1107, 1487
Bsp119I TTCGAA 1 cut(s) 1030
Bsp1286I GDGCHC 2 cut(s) 169, 315
Bsp1407I TGTACA 2 cut(s) 301, 488
Bsp143I GATC 3 cut(s) 187, 880, 1000
BspACI CCGC 4 cut(s) 179, 406, 749, 860
BspANI GGCC 5 cut(s) 258, 394, 613, 734, 1409
BspCNI CTCAG 4 cut(s) 718, 766, 883, 1248
BspLI GGNNCC 4 cut(s) 11, 416, 1120, 1454
BspMAI CTGCAG 1 cut(s) 1069
BspPI GGATC 3 cut(s) 195, 875, 1008
BspT104I TTCGAA 1 cut(s) 1030
BspT107I GGYRCC 3 cut(s) 9, 1118, 1452
BspTI CTTAAG 1 cut(s) 650
BsrFI RCCGGY 1 cut(s) 1121
BsrGI TGTACA 2 cut(s) 301, 488
BsrI ACTGG 2 cut(s) 460, 1410
BssAI RCCGGY 1 cut(s) 1121
BssECI CCNNGG 2 cut(s) 354, 684
BssMI GATC 3 cut(s) 187, 880, 1000
BssNAI GTATAC 1 cut(s) 1211
BssNI GRCGYC 2 cut(s) 10, 29
Bst1107I GTATAC 1 cut(s) 1211
Bst2UI CCWGG 1 cut(s) 391
Bst4CI ACNGT 2 cut(s) 493, 965
Bst6I CTCTTC 1 cut(s) 1336
BstACI GRCGYC 2 cut(s) 10, 29
BstAFI CTTAAG 1 cut(s) 650
BstAPI GCANNNNNTGC 1 cut(s) 1118
BstAUI TGTACA 2 cut(s) 301, 488
BstBAI YACGTR 1 cut(s) 422
BstBI TTCGAA 1 cut(s) 1030
BstC8I GCNNGC 2 cut(s) 527, 1123
BstDEI CTNAG 5 cut(s) 633, 726, 774, 870, 1235
BstDSI CCRYGG 1 cut(s) 354
BstEII GGTNACC 2 cut(s) 760, 1346
BstF5I GGATG 4 cut(s) 694, 748, 1048, 1428
BstH2I RGCGCY 1 cut(s) 13
BstHHI GCGC 1 cut(s) 12
BstKTI GATC 3 cut(s) 190, 883, 1003
BstMAI GTCTC 2 cut(s) 1239, 1312
BstMBI GATC 3 cut(s) 187, 880, 1000
BstMWI GCNNNNNNNGC 5 cut(s) 866, 1118, 1397, 1406, 1510
BstNI CCWGG 1 cut(s) 391
BstNSI RCATGY 2 cut(s) 340, 1209
BstPI GGTNACC 2 cut(s) 760, 1346
BstSCI CCNGG 1 cut(s) 389
BstSFI CTRYAG 1 cut(s) 1065
BstV1I GCAGC 5 cut(s) 105, 446, 661, 683, 1400
BstZ17I GTATAC 1 cut(s) 1211
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 5 cut(s) 258, 394, 613, 734, 1409
BtgI CCRYGG 1 cut(s) 354
BtgZI GCGATG 1 cut(s) 1494
BtsCI GGATG 4 cut(s) 694, 748, 1048, 1428
BtsI GCAGTG 2 cut(s) 1074, 1154
BtsIMutI CAGTG 4 cut(s) 167, 498, 1074, 1154
Cac8I GCNNGC 2 cut(s) 527, 1123
CfoI GCGC 1 cut(s) 12
Cfr10I RCCGGY 1 cut(s) 1121
Cfr13I GGNCC 2 cut(s) 257, 414
CseI GACGC 1 cut(s) 37
Csp6I GTAC 4 cut(s) 81, 302, 489, 1453
CviAII CATG 8 cut(s) 98, 197, 218, 305, 337, 1206, 1415, 1457
CviQI GTAC 4 cut(s) 81, 302, 489, 1453
DdeI CTNAG 5 cut(s) 633, 726, 774, 870, 1235
DinI GGCGCC 1 cut(s) 11
DpnI GATC 3 cut(s) 189, 882, 1002
DpnII GATC 3 cut(s) 187, 880, 1000
DraI TTTAAA 1 cut(s) 969
EaeI YGGCCR 1 cut(s) 392
Eam1104I CTCTTC 1 cut(s) 1336
EarI CTCTTC 1 cut(s) 1336
Ecl136II GAGCTC 1 cut(s) 313
Eco147I AGGCCT 1 cut(s) 734
Eco24I GRGCYC 2 cut(s) 169, 315
Eco47I GGWCC 1 cut(s) 414
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 143
Eco72I CACGTG 1 cut(s) 422
Eco88I CYCGRG 2 cut(s) 1107, 1487
Eco91I GGTNACC 2 cut(s) 760, 1346
EcoICRI GAGCTC 1 cut(s) 313
EcoO109I RGGNCCY 1 cut(s) 414
EcoO65I GGTNACC 2 cut(s) 760, 1346
EcoRII CCWGG 1 cut(s) 389
EcoT38I GRGCYC 2 cut(s) 169, 315
EgeI GGCGCC 1 cut(s) 11
EheI GGCGCC 1 cut(s) 11
Esp3I CGTCTC 1 cut(s) 1312
FaeI CATG 8 cut(s) 101, 200, 221, 308, 340, 1209, 1418, 1460
FalI AAGNNNNNCTT 4 cut(s) 626, 658, 884, 916
FaqI GGGAC 2 cut(s) 466, 1201
FatI CATG 8 cut(s) 97, 196, 217, 304, 336, 1205, 1414, 1456
FauI CCCGC 1 cut(s) 172
FblI GTMKAC 2 cut(s) 1210, 1317
Fnu4HI GCNGC 5 cut(s) 94, 460, 672, 675, 1389
FokI GGATG 4 cut(s) 701, 755, 1035, 1415
FriOI GRGCYC 2 cut(s) 169, 315
Fsp4HI GCNGC 5 cut(s) 94, 460, 672, 675, 1389
FspBI CTAG 3 cut(s) 447, 945, 1160
GlaI GCGC 1 cut(s) 11
GluI GCNGC 5 cut(s) 94, 460, 672, 675, 1389
HaeII RGCGCY 1 cut(s) 13
HaeIII GGCC 5 cut(s) 258, 394, 613, 734, 1409
HapII CCGG 1 cut(s) 1122
HgaI GACGC 1 cut(s) 37
HhaI GCGC 1 cut(s) 12
Hin1I GRCGYC 2 cut(s) 10, 29
Hin1II CATG 8 cut(s) 101, 200, 221, 308, 340, 1209, 1418, 1460
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HindIII AAGCTT 3 cut(s) 647, 898, 921
HinfI GANTC 9 cut(s) 4, 70, 154, 286, 350, 574, 1173, 1251, 1275
HpaII CCGG 1 cut(s) 1122
HphI GGTGA 6 cut(s) 194, 754, 817, 1217, 1282, 1358
Hpy166II GTNNAC 3 cut(s) 832, 1211, 1318
Hpy188III TCNNGA 4 cut(s) 269, 773, 1052, 1340
Hpy8I GTNNAC 3 cut(s) 832, 1211, 1318
Hpy99I CGWCG 3 cut(s) 939, 1449, 1452
HpyAV CCTTC 6 cut(s) 169, 472, 811, 1081, 1093, 1420
HpyCH4III ACNGT 2 cut(s) 493, 965
HpyCH4IV ACGT 3 cut(s) 421, 934, 1444
HpyCH4V TGCA 5 cut(s) 462, 677, 1067, 1154, 1355
HpyF10VI GCNNNNNNNGC 5 cut(s) 866, 1118, 1397, 1406, 1510
HpyF3I CTNAG 5 cut(s) 633, 726, 774, 870, 1235
HpySE526I ACGT 3 cut(s) 421, 934, 1444
Hsp92I GRCGYC 2 cut(s) 10, 29
Hsp92II CATG 8 cut(s) 101, 200, 221, 308, 340, 1209, 1418, 1460
HspAI GCGC 1 cut(s) 10
KasI GGCGCC 1 cut(s) 9
KpnI GGTACC 1 cut(s) 1456
KroI GCCGGC 1 cut(s) 1121
KroNI GCCGGC 1 cut(s) 1123
Kzo9I GATC 3 cut(s) 187, 880, 1000
LmnI GCTCC 2 cut(s) 271, 310
Lsp1109I GCAGC 5 cut(s) 105, 446, 661, 683, 1400
LweI GCATC 3 cut(s) 253, 1048, 1141
MaeI CTAG 3 cut(s) 447, 945, 1160
MaeII ACGT 3 cut(s) 421, 934, 1444
MaeIII GTNAC 5 cut(s) 25, 332, 760, 1102, 1346
MalI GATC 3 cut(s) 189, 882, 1002
MboI GATC 3 cut(s) 187, 880, 1000
MboII GAAGA 7 cut(s) 149, 390, 1044, 1310, 1323, 1382, 1506
MhlI GDGCHC 2 cut(s) 169, 315
MlsI TGGCCA 1 cut(s) 394
MluCI AATT 4 cut(s) 379, 484, 973, 1177
MluNI TGGCCA 1 cut(s) 394
Mly113I GGCGCC 1 cut(s) 10
MlyI GAGTC 5 cut(s) 344, 583, 1167, 1245, 1269
MmeI TCCRAC 2 cut(s) 27, 321
Mox20I TGGCCA 1 cut(s) 394
MroNI GCCGGC 1 cut(s) 1121
MscI TGGCCA 1 cut(s) 394
MseI TTAA 6 cut(s) 252, 651, 716, 912, 968, 1256
MslI CAYNNNNRTG 2 cut(s) 1013, 1053
Msp20I TGGCCA 1 cut(s) 394
MspA1I CMGCKG 2 cut(s) 674, 991
MspCI CTTAAG 1 cut(s) 650
MspI CCGG 1 cut(s) 1122
MspR9I CCNGG 1 cut(s) 391
MssI GTTTAAAC 1 cut(s) 969
MvaI CCWGG 1 cut(s) 391
MwoI GCNNNNNNNGC 5 cut(s) 866, 1118, 1397, 1406, 1510
NaeI GCCGGC 1 cut(s) 1123
NarI GGCGCC 1 cut(s) 10
NdeII GATC 3 cut(s) 187, 880, 1000
NgoMIV GCCGGC 1 cut(s) 1121
NlaIII CATG 8 cut(s) 101, 200, 221, 308, 340, 1209, 1418, 1460
NlaIV GGNNCC 4 cut(s) 11, 416, 1120, 1454
NmuCI GTSAC 5 cut(s) 25, 332, 760, 1102, 1346
NspI RCATGY 2 cut(s) 340, 1209
NspV TTCGAA 1 cut(s) 1030
OliI CACNNNNGTG 1 cut(s) 1013
PaeR7I CTCGAG 1 cut(s) 1487
PceI AGGCCT 1 cut(s) 734
PciI ACATGT 2 cut(s) 336, 1205
PdiI GCCGGC 1 cut(s) 1123
PfeI GAWTC 4 cut(s) 4, 70, 154, 286
PkrI GCNGC 5 cut(s) 95, 461, 673, 676, 1390
PleI GAGTC 5 cut(s) 344, 582, 1167, 1245, 1269
PluTI GGCGCC 1 cut(s) 13
PmaCI CACGTG 1 cut(s) 422
PmeI GTTTAAAC 1 cut(s) 969
PmlI CACGTG 1 cut(s) 422
PpsI GAGTC 5 cut(s) 344, 582, 1167, 1245, 1269
Ppu21I YACGTR 1 cut(s) 422
PpuMI RGGWCCY 1 cut(s) 414
PscI ACATGT 2 cut(s) 336, 1205
PshBI ATTAAT 1 cut(s) 912
Psp124BI GAGCTC 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 389
PspCI CACGTG 1 cut(s) 422
PspEI GGTNACC 2 cut(s) 760, 1346
PspGI CCWGG 1 cut(s) 389
PspN4I GGNNCC 4 cut(s) 11, 416, 1120, 1454
PspPI GGNCC 2 cut(s) 257, 414
PspPPI RGGWCCY 1 cut(s) 414
PspXI VCTCGAGB 1 cut(s) 1487
PstI CTGCAG 1 cut(s) 1069
PvuII CAGCTG 2 cut(s) 674, 991
RsaI GTAC 4 cut(s) 82, 303, 490, 1454
RsaNI GTAC 4 cut(s) 81, 302, 489, 1453
RseI CAYNNNNRTG 2 cut(s) 1013, 1053
SacI GAGCTC 1 cut(s) 315
SaqAI TTAA 6 cut(s) 252, 651, 716, 912, 968, 1256
SatI GCNGC 5 cut(s) 94, 460, 672, 675, 1389
Sau3AI GATC 3 cut(s) 187, 880, 1000
Sau96I GGNCC 2 cut(s) 257, 414
ScaI AGTACT 1 cut(s) 82
SchI GAGTC 5 cut(s) 344, 583, 1167, 1245, 1269
ScrFI CCNGG 1 cut(s) 391
SduI GDGCHC 2 cut(s) 169, 315
SfaNI GCATC 3 cut(s) 253, 1048, 1141
SfcI CTRYAG 1 cut(s) 1065
SfoI GGCGCC 1 cut(s) 11
Sfr274I CTCGAG 1 cut(s) 1487
SfuI TTCGAA 1 cut(s) 1030
SinI GGWCC 1 cut(s) 414
SlaI CTCGAG 1 cut(s) 1487
SmiMI CAYNNNNRTG 2 cut(s) 1013, 1053
SmlI CTYRAG 6 cut(s) 88, 168, 314, 650, 695, 1487
SmoI CTYRAG 6 cut(s) 88, 168, 314, 650, 695, 1487
Sse9I AATT 4 cut(s) 379, 484, 973, 1177
SseBI AGGCCT 1 cut(s) 734
SsiI CCGC 4 cut(s) 179, 406, 749, 860
SspDI GGCGCC 1 cut(s) 9
SspI AATATT 1 cut(s) 1260
SspMI CTAG 3 cut(s) 447, 945, 1160
SstI GAGCTC 1 cut(s) 315
StuI AGGCCT 1 cut(s) 734
StyD4I CCNGG 1 cut(s) 389
TaaI ACNGT 2 cut(s) 493, 965
TaiI ACGT 3 cut(s) 424, 937, 1447
TaqI TCGA 8 cut(s) 190, 284, 348, 659, 879, 1030, 1384, 1488
TaqII GACCGA 1 cut(s) 422
TasI AATT 4 cut(s) 379, 484, 973, 1177
TatI WGTACW 3 cut(s) 80, 301, 488
TfiI GAWTC 4 cut(s) 4, 70, 154, 286
Tru1I TTAA 6 cut(s) 252, 651, 716, 912, 968, 1256
Tru9I TTAA 6 cut(s) 252, 651, 716, 912, 968, 1256
TscAI CASTG 4 cut(s) 167, 498, 1074, 1161
TseFI GTSAC 5 cut(s) 25, 332, 760, 1102, 1346
TseI GCWGC 5 cut(s) 93, 459, 671, 674, 1388
Tsp45I GTSAC 5 cut(s) 25, 332, 760, 1102, 1346
TspGWI ACGGA 3 cut(s) 371, 483, 922
TspRI CASTG 4 cut(s) 167, 498, 1074, 1161
Vha464I CTTAAG 1 cut(s) 650
VpaK11BI GGWCC 1 cut(s) 414
VspI ATTAAT 1 cut(s) 912
XapI RAATTY 1 cut(s) 379
XceI RCATGY 2 cut(s) 340, 1209
XhoI CTCGAG 1 cut(s) 1487
XmiI GTMKAC 2 cut(s) 1210, 1317
XspI CTAG 3 cut(s) 447, 945, 1160
ZrmI AGTACT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.