Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0011147

Physical Location & Seq

Genomic Coordinates Forward (+)

21956532 .. 21959808

Gene Structure 3277 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1141400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2505 bp

ATGGGAAAGAATTTGAGAGACAAAAACCCTAACAATCCATCAGGGAATGAGCATTCATCAGGGTGCGTTTGGGGATTACTTCACATCATGAAGTATCACCACTGGCATTATGTCAAGAAAAGACAAGTGCACAAGAGGCGCCGCGGTAATAGACACACTGTCGGAGTTGGGATTCTGGGAAGCTGTACTGCAAATGCCTGTACGCCAGAAGACACAGATGCTAAGATTGATGAATCTACTGGTAAAGGAAAGACGATGCAGTCCATTCCAGCAGGCATACATTCTGTCAAGGCCCGAATAAAAGCACGGATTTCTGGAGAAATATCAAAGAAGAAGGGTAGGCTTAACCGGAGTTCAAGCTGCCCTGTACGATCACAATTGAAACAGCATCATTTCGAAGCACCACCTAACCTGCACCCGATTGCTGAAATGGTATTGAATGAGAAAAGTCCTAGAATTGCTCACCACGGCAATGAAAAATCTTGTGCTACAAGCAATCTGGCATCACCGCCCATGACATCATATGAAGGACCAATTAGTGACAACAATGATTGTTGTGGAGATAGTTGCCCACCAATCACTAGAGATCGTACCGTGCATGACCAGGTTAACGAGAATCAAAAGGATCACACATTTACTGAAGAAAAGCTGGATAATGACATAATGCAAAATATGTTCAAGAAGAAGTTGATAAACGTAAAAGAACTCGATGCAGATGCTTCCTTGCACCATCCGAAGGAGTATTTGGAGGCTTTGGATATAATCAATGTGAACAAGGAGTTATTAGTAAAGATTTTACATGACCCCGGCTCTCCTTTGGCTGTGCACTTCAATAATCAGCAAGCCGTCAGTGCAAAAATGGGTTTAACTAGGTCAGACTCATTCCCCGTCAAACTCAGGCACAAGCATGAGTTGACAGGGTCTCCTTTAAAAGAAGAAAGCAAGTCGAAAAACATCAGTAAAACAAAGAAGTTAGTAGGGTCGTCTTCTTTGAAATATGCTGGTGAACGCTCAATTCCATCGATCAGTGAGCACACTGCAGATGGGATTCTAGAGGTAAACCAAACAATTGCTGATAGTTCTAGTTCTGCTGAAAATTTGAGGAGCCGAGGTGAGAATCAAGTGTCAATTAAGCGGTTCAAGGATATTAGACAGAAAATAAAGCATGTAATCAAGGAGAGCAGACAAGAGAGACATATGATCGCCATGGATGCCATCCTCCACAAGGTTCCTCATGGCCAAAAGCTTGCTAAAGAAATGGAGAAAGAGATCATTAACCATTCGAAGGACCTTGCAATGAATAGAGAAGGTAAAGACAGTTCAGCAAGCAGTTTTGATAGCGATTATTCGATTTCTACCTTCAAGAAAAAGCAACAACGCCACATGAGAAGAGCATCATCACTCAATGCATCGCTAGATAAGTACTGTGAGTTTTACGAGTCTAGTTGCAACAGAGAAGCCAAATGCCATACAACATCCGAGAGATTGAAGTTGAGAAATGAAGGGGTAGGCTCGCCTTTACGCTCTGTCCCAAAAACGTTGGGGAGGATTTTTTCCTTGCCTGAAATGAAATCATATAACTATCAGAGTGAGGAATCTTCTGATGTGTTCTCTTCAGAATATCATATGCAGCTCGATGCGCCAGTAGAAAGTGAGATTAAAAAATTGGCTGATGTTGGTGAATTGAAGGCAGCTTCCGGGAATAAGATGGCATCAACATCAGTTGCAGACAGTGAGAAAAGTGCTCCAGTGTATTCAATTGTTGACGATATTGAAAACTTGAAAGAACCGTTTCCTATCTCTGTGCCCGAGTTCAACGTTTCCCTCTCCGAAGAAGAAACGGAAATAAAACCTAGAGATGAACAAGAAAACTTGGCCAAGCCAGGGCATGATTTTGTTATTGGTACGCCTAAGGCGGTTCACATAGATACAGAGAAGATTGAAAGTCCAAGGAAGCACTTGAGGTACGACATGACTCATCTCCGTGTTGATGACAGAGACATGGCTGAATTCAATTACGTACGAAATGTGTTGAAGCAATCTGGATTCAATGGGAATGAGTCCCTTGGGACATGGCATTCGGATGACCAGCCAGTTGATCGTTTGATGTATGGAGACGCGGAGGGCTGCTTGGTGCATGGTCTTGATTGTTCAGGAAATGAAGAAGGCAGGAAATGCGATCACTATCTTTTGTTTGATATAATCAATGAGGTGCTGATGGAGATATATGGTAGGTCATACAACTACTGTCCGATGGCGTTGTCTTCGCTATGTCACATTCCTCTAATGCCGGCAGGGAACCATGTTCTAAAGGAAGTTTGGACCCTTGTGAGTTGGTACTTGAGCTTGAGACCTGAGGTTGATCAATCACTGGATTATGTTGCGAGCAGGGATCTTGCGAAGAACGACGGATGGATGAACCTCCAGTTTGATTCTGAGTGCATAGGGATTGAGCTGGAGGACTTAATTTTTGATGAAATTTTAGAGGAAGTTCTCTGTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3741 DUF4378

Protein Analysis

835

Amino Acids

93.98

Weight (kDa)

6.53

Isoelectric Point (pI)

54.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3741	PF12552	228 - 271	1.2e-14	Protein of unknown function (DUF3741)
DUF4378	PF14309	670 - 830	1.1e-38	Domain of unknown function (DUF4378)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011147)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02390 AT5G02390 AT5G02390
fragaria_vesca	FvH4_6g13720
malus_domestica	MD04G1128800.v1.1 MD12G1141400.v1.1
prunus_persica	Prupe.6G254700_v2.0.a1
pyrus_communis	pycom04g11700 pycom12g13630
rosa_chinensis	RchiOBHm_Chr3g0466591
rosa_laevigata	RLG00000024561
rosa_multiflora	Rmu_sc0020778.1_g000002
rosa_roxburghii	Rroxscaffold_6G00414320
rosa_rugosa	Rorug03G0083000.1 Rorug03G0083100
rosa_samantha	Rh3AG129900 Rh3BG151300 Rh3CG151000 Rh3DG151900
rosa_wichuraiana	Rw3G012280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	259
Acc36I	ACCTGC	1 cut(s)	420
AccB1I	GGYRCC	1 cut(s)	138
AccII	CGCG	2 cut(s)	144, 2120
AciI	CCGC	6 cut(s)	142, 144, 509, 1135, 1916, 2120
AclI	AACGTT	2 cut(s)	1538, 1818
AclWI	GGATC	2 cut(s)	633, 2400
AcoI	YGGCCR	2 cut(s)	1237, 1875
AcsI	RAATTY	4 cut(s)	10, 1096, 2009, 2478
AcuI	CTGAAG	2 cut(s)	660, 1599
AcyI	GRCGYC	1 cut(s)	139
AfaI	GTAC	9 cut(s)	187, 202, 369, 592, 1424, 1906, 1967, 2022, 2339
AfiI	CCNNNNNNNGG	4 cut(s)	736, 1225, 1285, 1884
AhdI	GACNNNNNGTC	1 cut(s)	158
AjnI	CCWGG	2 cut(s)	603, 1882
AjuI	GAANNNNNNNTTGG	2 cut(s)	728, 760
AluBI	AGCT	8 cut(s)	183, 360, 649, 1246, 1633, 1694, 2346, 2455
AluI	AGCT	8 cut(s)	183, 360, 649, 1246, 1633, 1694, 2346, 2455
Alw21I	GWGCWC	4 cut(s)	132, 828, 1035, 1747
Alw26I	GTCTC	6 cut(s)	12, 927, 1186, 1992, 2109, 2344
Alw44I	GTGCAC	2 cut(s)	128, 824
AlwI	GGATC	2 cut(s)	633, 2400
Ama87I	CYCGRG	1 cut(s)	1808
AoxI	GGCC	3 cut(s)	291, 1237, 1875
ApaLI	GTGCAC	2 cut(s)	128, 824
ApeKI	GCWGC	4 cut(s)	360, 1630, 1691, 2127
ApoI	RAATTY	4 cut(s)	10, 1096, 2009, 2478
ArsI	GACNNNNNNTTYG	4 cut(s)	2062, 2094, 2453, 2485
Asp700I	GAANNNNTTC	1 cut(s)	1791
AspLEI	GCGC	2 cut(s)	141, 1642
AspS9I	GGNCC	4 cut(s)	292, 530, 1288, 2322
AsuC2I	CCSGG	2 cut(s)	807, 1699
AsuHPI	GGTGA	6 cut(s)	89, 455, 498, 1016, 1124, 1691
AsuII	TTCGAA	2 cut(s)	396, 1283
AvaI	CYCGRG	1 cut(s)	1808
AvaII	GGWCC	3 cut(s)	530, 1288, 2322
AxyI	CCTNAGG	2 cut(s)	1911, 2355
BaeGI	GKGCMC	3 cut(s)	132, 828, 1809
BalI	TGGCCA	2 cut(s)	1239, 1877
BanI	GGYRCC	1 cut(s)	138
BbsI	GAAGAC	3 cut(s)	216, 978, 2256
Bbv12I	GWGCWC	4 cut(s)	132, 828, 1035, 1747
BbvI	GCAGC	4 cut(s)	347, 1642, 1703, 2114
BccI	CCATC	9 cut(s)	46, 738, 1027, 1037, 1223, 1702, 2212, 2248, 2406
BceAI	ACGGC	2 cut(s)	484, 830
BcgI	CGANNNNNNTGC	2 cut(s)	698, 732
BciT130I	CCWGG	2 cut(s)	605, 1884
BclI	TGATCA	1 cut(s)	2362
BcnI	CCSGG	2 cut(s)	807, 1699
BcoDI	GTCTC	6 cut(s)	12, 927, 1186, 1992, 2109, 2344
BfaI	CTAG	8 cut(s)	453, 582, 870, 1052, 1083, 1415, 1443, 1854
BfmI	CTRYAG	1 cut(s)	1038
BfoI	RGCGCY	1 cut(s)	142
BfuAI	ACCTGC	1 cut(s)	420
BisI	GCNGC	5 cut(s)	142, 361, 1631, 1692, 2128
BlsI	GCNGC	5 cut(s)	143, 362, 1632, 1693, 2129
BmcAI	AGTACT	1 cut(s)	1424
Bme1390I	CCNGG	4 cut(s)	605, 807, 1699, 1884
Bme18I	GGWCC	3 cut(s)	530, 1288, 2322
BmeRI	GACNNNNNGTC	1 cut(s)	158
BmeT110I	CYCGRG	1 cut(s)	1808
BmgT120I	GGNCC	4 cut(s)	292, 530, 1288, 2322
BmiI	GGNNCC	5 cut(s)	140, 1106, 1230, 2300, 2324
BmrFI	CCNGG	4 cut(s)	605, 807, 1699, 1884
BpiI	GAAGAC	3 cut(s)	216, 978, 2256
BpmI	CTGGAG	4 cut(s)	336, 1731, 2408, 2477
Bpu14I	TTCGAA	2 cut(s)	396, 1283
BpuEI	CTTGAG	3 cut(s)	1981, 2362, 2368
BpuMI	CCSGG	2 cut(s)	807, 1699
Bsa29I	ATCGAT	1 cut(s)	1022
BsaAI	YACGTR	1 cut(s)	2020
BsaBI	GATNNNNATC	1 cut(s)	2184
BsaHI	GRCGYC	1 cut(s)	139
BsaI	GGTCTC	2 cut(s)	927, 2344
BsaJI	CCNNGG	8 cut(s)	142, 466, 805, 1108, 1206, 1883, 1949, 2065
BsaWI	WCCGGW	1 cut(s)	348
BsaXI	ACNNNNNCTCC	4 cut(s)	907, 937, 1096, 1126
Bsc4I	CCNNNNNNNGG	4 cut(s)	736, 1225, 1285, 1884
Bse118I	RCCGGY	1 cut(s)	2290
Bse1I	ACTGG	7 cut(s)	107, 244, 1643, 1748, 2093, 2376, 2425
Bse21I	CCTNAGG	2 cut(s)	1911, 2355
Bse3DI	GCAATG	2 cut(s)	478, 1302
Bse8I	GATNNNNATC	1 cut(s)	2184
BseBI	CCWGG	2 cut(s)	605, 1884
BseCI	ATCGAT	1 cut(s)	1022
BseDI	CCNNGG	8 cut(s)	142, 466, 805, 1108, 1206, 1883, 1949, 2065
BseGI	GGATG	7 cut(s)	730, 1215, 1216, 1475, 2089, 2417, 2421
BseJI	GATNNNNATC	1 cut(s)	2184
BseLI	CCNNNNNNNGG	4 cut(s)	736, 1225, 1285, 1884
BseMI	GCAATG	2 cut(s)	478, 1302
BseMII	CTCAG	3 cut(s)	910, 2346, 2427
BseNI	ACTGG	7 cut(s)	107, 244, 1643, 1748, 2093, 2376, 2425
BseRI	GAGGAG	1 cut(s)	1117
BseSI	GKGCMC	3 cut(s)	132, 828, 1809
BseXI	GCAGC	4 cut(s)	347, 1642, 1703, 2114
BsgI	GTGCAG	1 cut(s)	398
Bsh1236I	CGCG	2 cut(s)	144, 2120
BshFI	GGCC	3 cut(s)	293, 1239, 1877
BshNI	GGYRCC	1 cut(s)	138
BshVI	ATCGAT	1 cut(s)	1022
BsiHKAI	GWGCWC	4 cut(s)	132, 828, 1035, 1747
BsiHKCI	CYCGRG	1 cut(s)	1808
BsiSI	CCGG	4 cut(s)	349, 807, 1698, 2291
BsiWI	CGTACG	1 cut(s)	2020
BslFI	GGGAC	3 cut(s)	1514, 2047, 2083
BslI	CCNNNNNNNGG	4 cut(s)	736, 1225, 1285, 1884
BsmAI	GTCTC	6 cut(s)	12, 927, 1186, 1992, 2109, 2344
BsmBI	CGTCTC	1 cut(s)	2109
BsmFI	GGGAC	3 cut(s)	1514, 2047, 2083
BsmI	GAATGC	2 cut(s)	52, 2077
BsnI	GGCC	3 cut(s)	293, 1239, 1877
Bso31I	GGTCTC	2 cut(s)	927, 2344
BsoBI	CYCGRG	1 cut(s)	1808
Bsp119I	TTCGAA	2 cut(s)	396, 1283
Bsp1286I	GDGCHC	5 cut(s)	132, 828, 1035, 1747, 1809
Bsp19I	CCATGG	1 cut(s)	1206
BspACI	CCGC	6 cut(s)	142, 144, 509, 1135, 1916, 2120
BspANI	GGCC	3 cut(s)	293, 1239, 1877
BspCNI	CTCAG	3 cut(s)	909, 2347, 2428
BspDI	ATCGAT	1 cut(s)	1022
BspFNI	CGCG	2 cut(s)	144, 2120
BspHI	TCATGA	1 cut(s)	87
BspLI	GGNNCC	5 cut(s)	140, 1106, 1230, 2300, 2324
BspMAI	CTGCAG	1 cut(s)	1042
BspMI	ACCTGC	1 cut(s)	420
BspPI	GGATC	2 cut(s)	633, 2400
BspQI	GCTCTTC	1 cut(s)	1384
BspT104I	TTCGAA	2 cut(s)	396, 1283
BspT107I	GGYRCC	1 cut(s)	138
BspTNI	GGTCTC	2 cut(s)	927, 2344
BsrDI	GCAATG	2 cut(s)	478, 1302
BsrFI	RCCGGY	1 cut(s)	2290
BsrI	ACTGG	7 cut(s)	107, 244, 1643, 1748, 2093, 2376, 2425
BssAI	RCCGGY	1 cut(s)	2290
BssECI	CCNNGG	8 cut(s)	142, 466, 805, 1108, 1206, 1883, 1949, 2065
BssNI	GRCGYC	1 cut(s)	139
BssT1I	CCWWGG	3 cut(s)	1206, 1949, 2065
Bst2UI	CCWGG	2 cut(s)	605, 1884
Bst4CI	ACNGT	7 cut(s)	160, 595, 1319, 1427, 1733, 1791, 2249
Bst6I	CTCTTC	2 cut(s)	1384, 1618
BstACI	GRCGYC	1 cut(s)	139
BstAPI	GCANNNNNTGC	1 cut(s)	2175
BstBAI	YACGTR	1 cut(s)	2020
BstBI	TTCGAA	2 cut(s)	396, 1283
BstC8I	GCNNGC	7 cut(s)	274, 843, 1248, 1327, 1514, 2292, 2386
BstDEI	CTNAG	5 cut(s)	222, 896, 1911, 2355, 2436
BstDSI	CCRYGG	3 cut(s)	142, 466, 1206
BstENI	CCTNNNNNAGG	1 cut(s)	1223
BstF5I	GGATG	7 cut(s)	730, 1215, 1216, 1475, 2089, 2417, 2421
BstFNI	CGCG	2 cut(s)	144, 2120
BstH2I	RGCGCY	1 cut(s)	142
BstHHI	GCGC	2 cut(s)	141, 1642
BstMAI	GTCTC	6 cut(s)	12, 927, 1186, 1992, 2109, 2344
BstMWI	GCNNNNNNNGC	5 cut(s)	136, 851, 1211, 1639, 2175
BstNI	CCWGG	2 cut(s)	605, 1884
BstNSI	RCATGY	1 cut(s)	1169
BstSCI	CCNGG	4 cut(s)	603, 805, 1697, 1882
BstSFI	CTRYAG	1 cut(s)	1038
BstSLI	GKGCMC	3 cut(s)	132, 828, 1809
BstSNI	TACGTA	1 cut(s)	2020
BstUI	CGCG	2 cut(s)	144, 2120
BstV1I	GCAGC	4 cut(s)	347, 1642, 1703, 2114
BstV2I	GAAGAC	3 cut(s)	216, 978, 2256
BstX2I	RGATCY	1 cut(s)	2392
BstYI	RGATCY	1 cut(s)	2392
Bsu15I	ATCGAT	1 cut(s)	1022
Bsu36I	CCTNAGG	2 cut(s)	1911, 2355
BsuRI	GGCC	3 cut(s)	293, 1239, 1877
BsuTUI	ATCGAT	1 cut(s)	1022
BtgI	CCRYGG	3 cut(s)	142, 466, 1206
BtgZI	GCGATG	1 cut(s)	1395
BtsCI	GGATG	7 cut(s)	730, 1215, 1216, 1475, 2089, 2417, 2421
BtsI	GCAGTG	1 cut(s)	1035
BtsIMutI	CAGTG	8 cut(s)	100, 156, 856, 1033, 1035, 1738, 1755, 2369
BveI	ACCTGC	1 cut(s)	420
Cac8I	GCNNGC	7 cut(s)	274, 843, 1248, 1327, 1514, 2292, 2386
CciI	TCATGA	1 cut(s)	87
CfoI	GCGC	2 cut(s)	141, 1642
Cfr10I	RCCGGY	1 cut(s)	2290
Cfr13I	GGNCC	4 cut(s)	292, 530, 1288, 2322
Cfr42I	CCGCGG	1 cut(s)	145
ClaI	ATCGAT	1 cut(s)	1022
CseI	GACGC	1 cut(s)	2126
CsiI	ACCWGGT	1 cut(s)	603
Csp6I	GTAC	9 cut(s)	186, 201, 368, 591, 1423, 1905, 1966, 2021, 2338
CviQI	GTAC	9 cut(s)	186, 201, 368, 591, 1423, 1905, 1966, 2021, 2338
DdeI	CTNAG	5 cut(s)	222, 896, 1911, 2355, 2436
DinI	GGCGCC	1 cut(s)	140
DraI	TTTAAA	1 cut(s)	930
DrdI	GACNNNNNNGTC	1 cut(s)	259
DriI	GACNNNNNGTC	1 cut(s)	158
DseDI	GACNNNNNNGTC	1 cut(s)	259
EaeI	YGGCCR	2 cut(s)	1237, 1875
Eam1104I	CTCTTC	2 cut(s)	1384, 1618
Eam1105I	GACNNNNNGTC	1 cut(s)	158
EarI	CTCTTC	2 cut(s)	1384, 1618
Eco105I	TACGTA	1 cut(s)	2020
Eco130I	CCWWGG	3 cut(s)	1206, 1949, 2065
Eco31I	GGTCTC	2 cut(s)	927, 2344
Eco47I	GGWCC	3 cut(s)	530, 1288, 2322
Eco57I	CTGAAG	2 cut(s)	660, 1599
Eco81I	CCTNAGG	2 cut(s)	1911, 2355
Eco88I	CYCGRG	1 cut(s)	1808
EcoNI	CCTNNNNNAGG	1 cut(s)	1223
EcoO109I	RGGNCCY	1 cut(s)	1288
EcoRI	GAATTC	1 cut(s)	2009
EcoRII	CCWGG	2 cut(s)	603, 1882
EcoT14I	CCWWGG	3 cut(s)	1206, 1949, 2065
EcoT22I	ATGCAT	1 cut(s)	1411
EgeI	GGCGCC	1 cut(s)	140
EheI	GGCGCC	1 cut(s)	140
ErhI	CCWWGG	3 cut(s)	1206, 1949, 2065
Esp3I	CGTCTC	1 cut(s)	2109
FaqI	GGGAC	3 cut(s)	1514, 2047, 2083
FauNDI	CATATG	3 cut(s)	523, 1197, 1626
FbaI	TGATCA	1 cut(s)	2362
Fnu4HI	GCNGC	5 cut(s)	142, 361, 1631, 1692, 2128
FokI	GGATG	7 cut(s)	717, 1202, 1223, 1462, 2096, 2424, 2428
Fsp4HI	GCNGC	5 cut(s)	142, 361, 1631, 1692, 2128
FspBI	CTAG	8 cut(s)	453, 582, 870, 1052, 1083, 1415, 1443, 1854
GlaI	GCGC	2 cut(s)	140, 1641
GluI	GCNGC	5 cut(s)	142, 361, 1631, 1692, 2128
GsuI	CTGGAG	4 cut(s)	336, 1731, 2408, 2477
HaeII	RGCGCY	1 cut(s)	142
HaeIII	GGCC	3 cut(s)	293, 1239, 1877
HapII	CCGG	4 cut(s)	349, 807, 1698, 2291
HgaI	GACGC	1 cut(s)	2126
HhaI	GCGC	2 cut(s)	141, 1642
Hin1I	GRCGYC	1 cut(s)	139
Hin6I	GCGC	2 cut(s)	139, 1640
HinP1I	GCGC	2 cut(s)	139, 1640
HincII	GTYRAC	3 cut(s)	610, 915, 1765
HindII	GTYRAC	3 cut(s)	610, 915, 1765
HindIII	AAGCTT	1 cut(s)	1244
HpaI	GTTAAC	1 cut(s)	610
HpaII	CCGG	4 cut(s)	349, 807, 1698, 2291
HphI	GGTGA	6 cut(s)	89, 455, 498, 1016, 1124, 1691
Hpy166II	GTNNAC	9 cut(s)	130, 610, 772, 826, 915, 1007, 1060, 1765, 1921
Hpy188III	TCNNGA	9 cut(s)	88, 115, 315, 679, 1052, 1363, 2043, 2144, 2154
Hpy8I	GTNNAC	9 cut(s)	130, 610, 772, 826, 915, 1007, 1060, 1765, 1921
Hpy99I	CGWCG	1 cut(s)	2411
HpyAV	CCTTC	9 cut(s)	328, 521, 730, 1279, 1301, 1369, 1496, 1681, 2159
HpyCH4III	ACNGT	7 cut(s)	160, 595, 1319, 1427, 1733, 1791, 2249
HpyCH4IV	ACGT	4 cut(s)	696, 1538, 1818, 2019
HpyF10VI	GCNNNNNNNGC	5 cut(s)	136, 851, 1211, 1639, 2175
HpyF3I	CTNAG	5 cut(s)	222, 896, 1911, 2355, 2436
HpySE526I	ACGT	4 cut(s)	696, 1538, 1818, 2019
Hsp92I	GRCGYC	1 cut(s)	139
HspAI	GCGC	2 cut(s)	139, 1640
KasI	GGCGCC	1 cut(s)	138
KroI	GCCGGC	1 cut(s)	2290
KroNI	GCCGGC	1 cut(s)	2292
Ksp22I	TGATCA	1 cut(s)	2362
KspAI	GTTAAC	1 cut(s)	610
KspI	CCGCGG	1 cut(s)	145
LguI	GCTCTTC	1 cut(s)	1384
LmnI	GCTCC	2 cut(s)	1104, 1750
Lsp1109I	GCAGC	4 cut(s)	347, 1642, 1703, 2114
MabI	ACCWGGT	1 cut(s)	603
MaeI	CTAG	8 cut(s)	453, 582, 870, 1052, 1083, 1415, 1443, 1854
MaeII	ACGT	4 cut(s)	696, 1538, 1818, 2019
MaeIII	GTNAC	2 cut(s)	539, 2273
MfeI	CAATTG	3 cut(s)	377, 1068, 1758
MflI	RGATCY	1 cut(s)	2392
MhlI	GDGCHC	5 cut(s)	132, 828, 1035, 1747, 1809
MlsI	TGGCCA	2 cut(s)	1239, 1877
MluNI	TGGCCA	2 cut(s)	1239, 1877
Mly113I	GGCGCC	1 cut(s)	139
MlyI	GAGTC	4 cut(s)	872, 1448, 1969, 2069
MmeI	TCCRAC	1 cut(s)	142
Mox20I	TGGCCA	2 cut(s)	1239, 1877
Mph1103I	ATGCAT	1 cut(s)	1411
MroNI	GCCGGC	1 cut(s)	2290
MroXI	GAANNNNTTC	1 cut(s)	1791
MscI	TGGCCA	2 cut(s)	1239, 1877
MseI	TTAA	8 cut(s)	345, 609, 866, 929, 1131, 1275, 1659, 2465
MslI	CAYNNNNRTG	5 cut(s)	61, 471, 906, 1983, 2082
Msp20I	TGGCCA	2 cut(s)	1239, 1877
MspA1I	CMGCKG	1 cut(s)	144
MspI	CCGG	4 cut(s)	349, 807, 1698, 2291
MspR9I	CCNGG	4 cut(s)	605, 807, 1699, 1884
MunI	CAATTG	3 cut(s)	377, 1068, 1758
Mva1269I	GAATGC	2 cut(s)	52, 2077
MvaI	CCWGG	2 cut(s)	605, 1884
MvnI	CGCG	2 cut(s)	144, 2120
MwoI	GCNNNNNNNGC	5 cut(s)	136, 851, 1211, 1639, 2175
NaeI	GCCGGC	1 cut(s)	2292
NarI	GGCGCC	1 cut(s)	139
NciI	CCSGG	2 cut(s)	807, 1699
NcoI	CCATGG	1 cut(s)	1206
NdeI	CATATG	3 cut(s)	523, 1197, 1626
NgoMIV	GCCGGC	1 cut(s)	2290
NlaIV	GGNNCC	5 cut(s)	140, 1106, 1230, 2300, 2324
NmeAIII	GCCGAG	1 cut(s)	1133
NmuCI	GTSAC	2 cut(s)	539, 2273
NsiI	ATGCAT	1 cut(s)	1411
NspI	RCATGY	1 cut(s)	1169
NspV	TTCGAA	2 cut(s)	396, 1283
PagI	TCATGA	1 cut(s)	87
PciSI	GCTCTTC	1 cut(s)	1384
PctI	GAATGC	2 cut(s)	52, 2077
PdiI	GCCGGC	1 cut(s)	2292
PdmI	GAANNNNTTC	1 cut(s)	1791
PfeI	GAWTC	8 cut(s)	172, 233, 616, 1048, 1117, 1595, 2046, 2432
Pfl23II	CGTACG	1 cut(s)	2020
PflFI	GACNNNGTC	1 cut(s)	919
PfoI	TCCNGGA	1 cut(s)	1697
PkrI	GCNGC	5 cut(s)	143, 362, 1632, 1693, 2129
PleI	GAGTC	4 cut(s)	872, 1447, 1969, 2068
PluTI	GGCGCC	1 cut(s)	142
PpsI	GAGTC	4 cut(s)	872, 1447, 1969, 2068
Ppu21I	YACGTR	1 cut(s)	2020
PpuMI	RGGWCCY	1 cut(s)	1288
Psp1406I	AACGTT	2 cut(s)	1538, 1818
Psp5II	RGGWCCY	1 cut(s)	1288
Psp6I	CCWGG	2 cut(s)	603, 1882
PspGI	CCWGG	2 cut(s)	603, 1882
PspLI	CGTACG	1 cut(s)	2020
PspN4I	GGNNCC	5 cut(s)	140, 1106, 1230, 2300, 2324
PspPI	GGNCC	4 cut(s)	292, 530, 1288, 2322
PspPPI	RGGWCCY	1 cut(s)	1288
PsrI	GAACNNNNNNTAC	2 cut(s)	1303, 1335
PstI	CTGCAG	1 cut(s)	1042
PsuI	RGATCY	1 cut(s)	2392
PsyI	GACNNNGTC	1 cut(s)	919
RsaI	GTAC	9 cut(s)	187, 202, 369, 592, 1424, 1906, 1967, 2022, 2339
RsaNI	GTAC	9 cut(s)	186, 201, 368, 591, 1423, 1905, 1966, 2021, 2338
RseI	CAYNNNNRTG	5 cut(s)	61, 471, 906, 1983, 2082
SacII	CCGCGG	1 cut(s)	145
SapI	GCTCTTC	1 cut(s)	1384
SaqAI	TTAA	8 cut(s)	345, 609, 866, 929, 1131, 1275, 1659, 2465
SatI	GCNGC	5 cut(s)	142, 361, 1631, 1692, 2128
Sau96I	GGNCC	4 cut(s)	292, 530, 1288, 2322
ScaI	AGTACT	1 cut(s)	1424
SchI	GAGTC	4 cut(s)	872, 1448, 1969, 2069
ScrFI	CCNGG	4 cut(s)	605, 807, 1699, 1884
SduI	GDGCHC	5 cut(s)	132, 828, 1035, 1747, 1809
SexAI	ACCWGGT	1 cut(s)	603
SfcI	CTRYAG	1 cut(s)	1038
SfoI	GGCGCC	1 cut(s)	140
Sfr303I	CCGCGG	1 cut(s)	145
SfuI	TTCGAA	2 cut(s)	396, 1283
SgrBI	CCGCGG	1 cut(s)	145
SinI	GGWCC	3 cut(s)	530, 1288, 2322
SmiMI	CAYNNNNRTG	5 cut(s)	61, 471, 906, 1983, 2082
SmlI	CTYRAG	3 cut(s)	1960, 2341, 2347
SmoI	CTYRAG	3 cut(s)	1960, 2341, 2347
SnaBI	TACGTA	1 cut(s)	2020
SsiI	CCGC	6 cut(s)	142, 144, 509, 1135, 1916, 2120
SspDI	GGCGCC	1 cut(s)	138
SspMI	CTAG	8 cut(s)	453, 582, 870, 1052, 1083, 1415, 1443, 1854
StyD4I	CCNGG	4 cut(s)	603, 805, 1697, 1882
StyI	CCWWGG	3 cut(s)	1206, 1949, 2065
TaaI	ACNGT	7 cut(s)	160, 595, 1319, 1427, 1733, 1791, 2249
TaiI	ACGT	4 cut(s)	699, 1541, 1821, 2022
TaqI	TCGA	7 cut(s)	396, 708, 947, 1022, 1283, 1349, 1635
TatI	WGTACW	2 cut(s)	185, 1422
TauI	GCSGC	1 cut(s)	144
TfiI	GAWTC	8 cut(s)	172, 233, 616, 1048, 1117, 1595, 2046, 2432
Tru1I	TTAA	8 cut(s)	345, 609, 866, 929, 1131, 1275, 1659, 2465
Tru9I	TTAA	8 cut(s)	345, 609, 866, 929, 1131, 1275, 1659, 2465
TscAI	CASTG	8 cut(s)	107, 163, 856, 1033, 1042, 1738, 1755, 2376
TseFI	GTSAC	2 cut(s)	539, 2273
TseI	GCWGC	4 cut(s)	360, 1630, 1691, 2127
Tsp45I	GTSAC	2 cut(s)	539, 2273
TspGWI	ACGGA	4 cut(s)	322, 1856, 1973, 2424
TspRI	CASTG	8 cut(s)	107, 163, 856, 1033, 1042, 1738, 1755, 2376
Tth111I	GACNNNGTC	1 cut(s)	919
VneI	GTGCAC	2 cut(s)	128, 824
VpaK11BI	GGWCC	3 cut(s)	530, 1288, 2322
XagI	CCTNNNNNAGG	1 cut(s)	1223
XapI	RAATTY	4 cut(s)	10, 1096, 2009, 2478
XbaI	TCTAGA	1 cut(s)	1051
XceI	RCATGY	1 cut(s)	1169
XmnI	GAANNNNTTC	1 cut(s)	1791
XspI	CTAG	8 cut(s)	453, 582, 870, 1052, 1083, 1415, 1443, 1854
ZrmI	AGTACT	1 cut(s)	1424
Zsp2I	ATGCAT	1 cut(s)	1411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.