MD04G1129500.v1.1
MYB Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0006575
Physical Location & Seq
Reverse (-)
21561452 .. 21565413
3962 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1129500.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGAAGGAGAGACAGCGTTGGAGTGCTGAAGAGGACGCTTTGTTACGTGCATATGTGAAGCAATATGGACCAAGGGAGTGGAACCTTGTATCGCAGCGCATGAACACACCCCTAGACAGGGATGCTAAATCTTGCTTAGAAAGGTGGAAGAATTATCTCAAACCCGGCATTAAGAAAGGATCCCTAACTGAAGAGGAGCAGCGCCTTGTCATTTGTCTTCAAGCCAAACACGGTAATAAGTGGAAGAAAATTGCTGCTGAAGTCCCTGGTCGTACGGCTAAGAGATTGGGAAAGTGGTGGGAAGTGTTCAAAGAGAAGCAGCAAAGAGAACAGAAGAACAAGAAGATAACTGACCCTATTGTGGAGGGTAAATACGATACAATACTTGAAACTTTTGCCGAGAAGTTGGTGAAGGAGCGTGCGCCAACCTATCTCATGGCTACTTCAAACGGGGCCTATCTTCATACGGAAACATCTTCTCCAGCGCCAACGATTCTTCCTCCTTGGCTTTCTAATTCCAGTGTGTCCCCCAATGTGAGGCCACCATCTCCTTCTGTTACCCTGAGTCTGTCCCCGACAGTGGCACCCTCTCCGCCAATCCCTTGGCTGCAGCAGGATCGAGGATCAGATGGTAGTTTTGTTGTGGGAAATTTGCCACATCATGGTGTAGTTCCCGCTTGTGGGGAGAACCTAGTGATATCTGAGTTGGTGGAGTGCTCCAGAGAGTTGGAGGAAATGCACCGTGCTTGGGCAGTGCATAAGAAGGAAGCTTCGTGGAGGTTAAGAAGGGTGGAGTTGCAACTGGATTCGGAGAAGGCTTGTCGGAGGAGGGAGAAGATGGAAGAGATTGAAGCCAAGGTGAAGGCTCTAAGGGAAGAGCAGAAGGCTGCTCTAGACAGGATCGAAGCAGAATACAGGGAACAGTTAGCAGGGCTAAGGAGGGATGCAGAAGCAAAGGAGCAGAAGTTGGCTGAGCAATGGGCCGCGAAGCATTTGCGTCTCTCCCAGTTTCTCGAGCAGATGGGAGGCAGACCAAGGATTGTTGAGCCTAACGGCCGGTGA

Protein Analysis

354

Amino Acids

40.56

Weight (kDa)

9.38

Isoelectric Point (pI)

66.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 4 - 53 5.8e-13 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 7 - 67 3.5e-14 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 59 - 94 3.4e-09 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 63 - 111 5.1e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 583
AccB7I CCANNNNNTGG 1 cut(s) 662
AccII CGCG 1 cut(s) 986
AciI CCGC 3 cut(s) 593, 675, 984
AclWI GGATC 5 cut(s) 174, 187, 624, 631, 908
AcoI YGGCCR 1 cut(s) 1054
AcsI RAATTY 1 cut(s) 649
AcuI CTGAAG 3 cut(s) 48, 210, 279
AfaI GTAC 1 cut(s) 274
AfiI CCNNNNNNNGG 9 cut(s) 117, 118, 361, 537, 580, 662, 680, 681, 748
AgsI TTSAA 5 cut(s) 221, 310, 389, 447, 851
AjnI CCWGG 1 cut(s) 265
AluBI AGCT 1 cut(s) 770
AluI AGCT 1 cut(s) 770
Alw21I GWGCWC 1 cut(s) 719
Alw26I GTCTC 2 cut(s) 4, 1004
AlwI GGATC 5 cut(s) 174, 187, 624, 631, 908
Ama87I CYCGRG 1 cut(s) 1013
AoxI GGCC 4 cut(s) 453, 539, 981, 1054
ApeKI GCWGC 7 cut(s) 94, 199, 254, 319, 607, 610, 887
ApoI RAATTY 1 cut(s) 649
AspLEI GCGC 4 cut(s) 99, 204, 424, 487
AspS9I GGNCC 3 cut(s) 68, 453, 981
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 2 cut(s) 421, 871
AvaI CYCGRG 1 cut(s) 1013
AvaII GGWCC 1 cut(s) 68
BamHI GGATCC 1 cut(s) 179
BanI GGYRCC 1 cut(s) 583
BbsI GAAGAC 1 cut(s) 209
Bbv12I GWGCWC 1 cut(s) 719
BbvI GCAGC 7 cut(s) 106, 211, 241, 331, 594, 622, 874
BccI CCATC 4 cut(s) 553, 623, 832, 1015
BceAI ACGGC 1 cut(s) 291
BcgI CGANNNNNNTGC 4 cut(s) 599, 633, 976, 1010
BciT130I CCWGG 1 cut(s) 267
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 2 cut(s) 4, 1004
BfaI CTAG 3 cut(s) 113, 692, 893
BfmI CTRYAG 1 cut(s) 608
BfoI RGCGCY 2 cut(s) 205, 488
BisI GCNGC 8 cut(s) 95, 200, 255, 320, 608, 611, 888, 984
BlpI GCTNAGC 1 cut(s) 972
BlsI GCNGC 8 cut(s) 96, 201, 256, 321, 609, 612, 889, 985
Bme1390I CCNGG 2 cut(s) 165, 267
Bme18I GGWCC 1 cut(s) 68
BmeT110I CYCGRG 1 cut(s) 1013
BmgT120I GGNCC 3 cut(s) 68, 453, 981
BmiI GGNNCC 4 cut(s) 83, 181, 454, 585
BmrFI CCNGG 2 cut(s) 165, 267
BmrI ACTGGG 1 cut(s) 1000
BmsI GCATC 2 cut(s) 112, 934
BmuI ACTGGG 1 cut(s) 1000
BpiI GAAGAC 1 cut(s) 209
BpmI CTGGAG 2 cut(s) 465, 703
Bpu10I CCTNAGC 1 cut(s) 935
Bpu1102I GCTNAGC 1 cut(s) 972
BpuMI CCSGG 1 cut(s) 165
BsaAI YACGTR 1 cut(s) 47
BsaJI CCNNGG 6 cut(s) 71, 265, 503, 602, 855, 1034
BsaXI ACNNNNNCTCC 4 cut(s) 463, 493, 950, 980
Bsc4I CCNNNNNNNGG 9 cut(s) 117, 118, 361, 537, 580, 662, 680, 681, 748
Bse118I RCCGGY 1 cut(s) 1056
Bse1I ACTGG 3 cut(s) 519, 807, 1006
Bse3DI GCAATG 1 cut(s) 983
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 6 cut(s) 71, 265, 503, 602, 855, 1034
BseGI GGATG 2 cut(s) 127, 949
BseLI CCNNNNNNNGG 9 cut(s) 117, 118, 361, 537, 580, 662, 680, 681, 748
BseMI GCAATG 1 cut(s) 983
BseMII CTCAG 3 cut(s) 554, 693, 963
BseNI ACTGG 3 cut(s) 519, 807, 1006
BseRI GAGGAG 2 cut(s) 209, 841
BseX3I CGGCCG 1 cut(s) 1054
BseXI GCAGC 7 cut(s) 106, 211, 241, 331, 594, 622, 874
Bsh1236I CGCG 1 cut(s) 986
Bsh1285I CGRYCG 1 cut(s) 1057
BshFI GGCC 4 cut(s) 455, 541, 983, 1056
BshNI GGYRCC 1 cut(s) 583
BsiEI CGRYCG 1 cut(s) 1057
BsiHKAI GWGCWC 1 cut(s) 719
BsiHKCI CYCGRG 1 cut(s) 1013
BsiSI CCGG 2 cut(s) 165, 1057
BsiWI CGTACG 1 cut(s) 272
BslFI GGGAC 3 cut(s) 248, 511, 556
BslI CCNNNNNNNGG 9 cut(s) 117, 118, 361, 537, 580, 662, 680, 681, 748
BsmAI GTCTC 2 cut(s) 4, 1004
BsmBI CGTCTC 1 cut(s) 1004
BsmFI GGGAC 3 cut(s) 248, 511, 556
BsnI GGCC 4 cut(s) 455, 541, 983, 1056
BsoBI CYCGRG 1 cut(s) 1013
Bsp1286I GDGCHC 1 cut(s) 719
Bsp143I GATC 4 cut(s) 179, 616, 623, 900
Bsp1720I GCTNAGC 1 cut(s) 972
BspACI CCGC 3 cut(s) 593, 675, 984
BspANI GGCC 4 cut(s) 455, 541, 983, 1056
BspCNI CTCAG 3 cut(s) 555, 694, 964
BspFNI CGCG 1 cut(s) 986
BspLI GGNNCC 4 cut(s) 83, 181, 454, 585
BspMAI CTGCAG 1 cut(s) 612
BspPI GGATC 5 cut(s) 174, 187, 624, 631, 908
BspQI GCTCTTC 1 cut(s) 870
BspT107I GGYRCC 1 cut(s) 583
BsrDI GCAATG 1 cut(s) 983
BsrFI RCCGGY 1 cut(s) 1056
BsrI ACTGG 3 cut(s) 519, 807, 1006
BssAI RCCGGY 1 cut(s) 1056
BssECI CCNNGG 6 cut(s) 71, 265, 503, 602, 855, 1034
BssMI GATC 4 cut(s) 179, 616, 623, 900
BssT1I CCWWGG 5 cut(s) 71, 503, 602, 855, 1034
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 4 cut(s) 233, 580, 743, 924
Bst6I CTCTTC 4 cut(s) 24, 186, 837, 870
BstBAI YACGTR 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 420
BstDEI CTNAG 7 cut(s) 136, 279, 563, 702, 869, 935, 972
BstF5I GGATG 2 cut(s) 127, 949
BstFNI CGCG 1 cut(s) 986
BstH2I RGCGCY 2 cut(s) 205, 488
BstHHI GCGC 4 cut(s) 99, 204, 424, 487
BstKTI GATC 4 cut(s) 182, 619, 626, 903
BstMAI GTCTC 2 cut(s) 4, 1004
BstMBI GATC 4 cut(s) 179, 616, 623, 900
BstMCI CGRYCG 1 cut(s) 1057
BstNI CCWGG 1 cut(s) 267
BstSCI CCNGG 2 cut(s) 163, 265
BstSFI CTRYAG 1 cut(s) 608
BstUI CGCG 1 cut(s) 986
BstV1I GCAGC 7 cut(s) 106, 211, 241, 331, 594, 622, 874
BstV2I GAAGAC 1 cut(s) 209
BstX2I RGATCY 1 cut(s) 179
BstXI CCANNNNNNTGG 3 cut(s) 78, 603, 727
BstYI RGATCY 1 cut(s) 179
BstZI CGGCCG 1 cut(s) 1054
BsuRI GGCC 4 cut(s) 455, 541, 983, 1056
BtsCI GGATG 2 cut(s) 127, 949
BtsI GCAGTG 1 cut(s) 759
BtsIMutI CAGTG 3 cut(s) 526, 585, 759
Cac8I GCNNGC 1 cut(s) 420
CfoI GCGC 4 cut(s) 99, 204, 424, 487
Cfr10I RCCGGY 1 cut(s) 1056
Cfr13I GGNCC 3 cut(s) 68, 453, 981
CseI GACGC 2 cut(s) 44, 986
Csp6I GTAC 1 cut(s) 273
CviAII CATG 3 cut(s) 100, 436, 662
CviQI GTAC 1 cut(s) 273
DdeI CTNAG 7 cut(s) 136, 279, 563, 702, 869, 935, 972
DpnI GATC 4 cut(s) 181, 618, 625, 902
DpnII GATC 4 cut(s) 179, 616, 623, 900
EaeI YGGCCR 1 cut(s) 1054
EagI CGGCCG 1 cut(s) 1054
Eam1104I CTCTTC 4 cut(s) 24, 186, 837, 870
EarI CTCTTC 4 cut(s) 24, 186, 837, 870
EciI GGCGGA 1 cut(s) 582
EclXI CGGCCG 1 cut(s) 1054
Eco130I CCWWGG 5 cut(s) 71, 503, 602, 855, 1034
Eco32I GATATC 1 cut(s) 699
Eco47I GGWCC 1 cut(s) 68
Eco52I CGGCCG 1 cut(s) 1054
Eco57I CTGAAG 3 cut(s) 48, 210, 279
Eco88I CYCGRG 1 cut(s) 1013
EcoO109I RGGNCCY 1 cut(s) 453
EcoRII CCWGG 1 cut(s) 265
EcoRV GATATC 1 cut(s) 699
EcoT14I CCWWGG 5 cut(s) 71, 503, 602, 855, 1034
ErhI CCWWGG 5 cut(s) 71, 503, 602, 855, 1034
Esp3I CGTCTC 1 cut(s) 1004
FaeI CATG 3 cut(s) 103, 439, 665
FaiI YATR 8 cut(s) 52, 54, 66, 101, 437, 465, 663, 759
FaqI GGGAC 3 cut(s) 248, 511, 556
FatI CATG 3 cut(s) 99, 435, 661
FauI CCCGC 1 cut(s) 682
FauNDI CATATG 1 cut(s) 52
Fnu4HI GCNGC 8 cut(s) 95, 200, 255, 320, 608, 611, 888, 984
FokI GGATG 2 cut(s) 134, 956
Fsp4HI GCNGC 8 cut(s) 95, 200, 255, 320, 608, 611, 888, 984
FspBI CTAG 3 cut(s) 113, 692, 893
GlaI GCGC 4 cut(s) 98, 203, 423, 486
GluI GCNGC 8 cut(s) 95, 200, 255, 320, 608, 611, 888, 984
GsuI CTGGAG 2 cut(s) 465, 703
HaeII RGCGCY 2 cut(s) 205, 488
HaeIII GGCC 4 cut(s) 455, 541, 983, 1056
HapII CCGG 2 cut(s) 165, 1057
HgaI GACGC 2 cut(s) 44, 986
HhaI GCGC 4 cut(s) 99, 204, 424, 487
Hin1II CATG 3 cut(s) 103, 439, 665
Hin6I GCGC 4 cut(s) 97, 202, 422, 485
HinP1I GCGC 4 cut(s) 97, 202, 422, 485
HindIII AAGCTT 1 cut(s) 768
HinfI GANTC 3 cut(s) 493, 565, 806
HpaII CCGG 2 cut(s) 165, 1057
HphI GGTGA 2 cut(s) 421, 871
Hpy188I TCNGA 4 cut(s) 628, 703, 811, 825
Hpy188III TCNNGA 3 cut(s) 720, 893, 1013
HpyAV CCTTC 7 cut(s) 406, 561, 757, 780, 808, 856, 877
HpyCH4III ACNGT 4 cut(s) 233, 580, 743, 924
HpyCH4IV ACGT 1 cut(s) 46
HpyCH4V TGCA 6 cut(s) 50, 610, 739, 757, 799, 947
HpyF3I CTNAG 7 cut(s) 136, 279, 563, 702, 869, 935, 972
HpySE526I ACGT 1 cut(s) 46
Hsp92II CATG 3 cut(s) 103, 439, 665
HspAI GCGC 4 cut(s) 97, 202, 422, 485
Kzo9I GATC 4 cut(s) 179, 616, 623, 900
LguI GCTCTTC 1 cut(s) 870
LmnI GCTCC 4 cut(s) 196, 415, 722, 958
Lsp1109I GCAGC 7 cut(s) 106, 211, 241, 331, 594, 622, 874
LweI GCATC 2 cut(s) 112, 934
MaeI CTAG 3 cut(s) 113, 692, 893
MaeII ACGT 1 cut(s) 46
MaeIII GTNAC 2 cut(s) 42, 556
MalI GATC 4 cut(s) 181, 618, 625, 902
MboI GATC 4 cut(s) 179, 616, 623, 900
MflI RGATCY 1 cut(s) 179
MhlI GDGCHC 1 cut(s) 719
MluCI AATT 4 cut(s) 151, 249, 514, 649
MlyI GAGTC 1 cut(s) 574
MmeI TCCRAC 2 cut(s) 708, 803
MseI TTAA 2 cut(s) 171, 782
MslI CAYNNNNRTG 1 cut(s) 663
MspI CCGG 2 cut(s) 165, 1057
MspR9I CCNGG 2 cut(s) 165, 267
MvaI CCWGG 1 cut(s) 267
MvnI CGCG 1 cut(s) 986
NciI CCSGG 1 cut(s) 165
NdeI CATATG 1 cut(s) 52
NdeII GATC 4 cut(s) 179, 616, 623, 900
NlaIII CATG 3 cut(s) 103, 439, 665
NlaIV GGNNCC 4 cut(s) 83, 181, 454, 585
NmeAIII GCCGAG 1 cut(s) 424
PaeR7I CTCGAG 1 cut(s) 1013
PciSI GCTCTTC 1 cut(s) 870
PfeI GAWTC 2 cut(s) 493, 806
Pfl23II CGTACG 1 cut(s) 272
PflMI CCANNNNNTGG 1 cut(s) 662
PkrI GCNGC 8 cut(s) 96, 201, 256, 321, 609, 612, 889, 985
PleI GAGTC 1 cut(s) 573
PpsI GAGTC 1 cut(s) 573
Ppu21I YACGTR 1 cut(s) 47
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspLI CGTACG 1 cut(s) 272
PspN4I GGNNCC 4 cut(s) 83, 181, 454, 585
PspPI GGNCC 3 cut(s) 68, 453, 981
PstI CTGCAG 1 cut(s) 612
PsuI RGATCY 1 cut(s) 179
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
RseI CAYNNNNRTG 1 cut(s) 663
SapI GCTCTTC 1 cut(s) 870
SaqAI TTAA 2 cut(s) 171, 782
SatI GCNGC 8 cut(s) 95, 200, 255, 320, 608, 611, 888, 984
Sau3AI GATC 4 cut(s) 179, 616, 623, 900
Sau96I GGNCC 3 cut(s) 68, 453, 981
SchI GAGTC 1 cut(s) 574
ScrFI CCNGG 2 cut(s) 165, 267
SduI GDGCHC 1 cut(s) 719
SetI ASST 8 cut(s) 49, 87, 146, 431, 693, 772, 782, 861
SfaNI GCATC 2 cut(s) 112, 934
SfcI CTRYAG 1 cut(s) 608
Sfr274I CTCGAG 1 cut(s) 1013
SinI GGWCC 1 cut(s) 68
SlaI CTCGAG 1 cut(s) 1013
SmiMI CAYNNNNRTG 1 cut(s) 663
SmlI CTYRAG 1 cut(s) 1013
SmoI CTYRAG 1 cut(s) 1013
Sse9I AATT 4 cut(s) 151, 249, 514, 649
SsiI CCGC 3 cut(s) 593, 675, 984
SspMI CTAG 3 cut(s) 113, 692, 893
StyD4I CCNGG 2 cut(s) 163, 265
StyI CCWWGG 5 cut(s) 71, 503, 602, 855, 1034
TaaI ACNGT 4 cut(s) 233, 580, 743, 924
TaiI ACGT 1 cut(s) 49
TaqI TCGA 3 cut(s) 619, 903, 1014
TasI AATT 4 cut(s) 151, 249, 514, 649
TauI GCSGC 1 cut(s) 986
TfiI GAWTC 2 cut(s) 493, 806
Tru1I TTAA 2 cut(s) 171, 782
Tru9I TTAA 2 cut(s) 171, 782
TscAI CASTG 3 cut(s) 526, 585, 759
TseI GCWGC 7 cut(s) 94, 199, 254, 319, 607, 610, 887
TspDTI ATGAA 3 cut(s) 17, 116, 452
TspGWI ACGGA 1 cut(s) 482
TspRI CASTG 3 cut(s) 526, 585, 759
Van91I CCANNNNNTGG 1 cut(s) 662
VpaK11BI GGWCC 1 cut(s) 68
XapI RAATTY 1 cut(s) 649
XbaI TCTAGA 1 cut(s) 892
XhoI CTCGAG 1 cut(s) 1013
XspI CTAG 3 cut(s) 113, 692, 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.