MD04G1131400.v1.1

Belongs to the DHHC palmitoyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007849
Physical Location & Seq
Reverse (-)
21827137 .. 21831977
4841 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1131400.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGAAGGGTAATACCTTAAATCAGAAACGACCGGCGGCACCATCTTTGATTTTGCAAACTCGGTCACCGACTCACCAACCTCCAAATCCTCGCATTCTGCCAGCTGAAAACGACACCCAACCCGCGGGAGACGCCGTCGTTTTGACGCCGGTGCCTCCTCCAGGCCCTCCGCCACCGCCGGAGAATATTCCAGACACAATGACGCGAAGCCTCGGCTTCTCCCTGCCCGTAACCGTCGTCGTTTTGGCAATCGCATACATTTACTTCTCCACGGTCTTCATTTTCATAGACCGTTGGTTCGGTCTCATGTCGTCGCCGGGTTTGATGAACGCCGTCGTTTTCACCGGCGTGGCCGTCATGTGCGTCTTCAATTACTCGGCTTCCGTCTTCAGGGATCCGGGTCGGGTTCCCTCCACGTACATGCCCGACGTCGAAGATTCGGGTAACCCTACGCACGAGATCAAGCGCAAGGGAGGGGATTTAAGATATTGCCAAAAGTGTTCTCACTATAAGCCAGCTCGTGCACATCATTGTCGTGTATGCAAAAGATGTGTTTTGCGCATGGATCATCATTGCATATGGATTAATAACTGCGTTGGCCATGCAAACTATAAGGTCTTTTTCATCTTTGTTGTGTATGCTGTAATAGCATGCCTCTATTCCCTGGTTTTGCTCGTCGGTAGCCTAACTTATGATCCCGAAAAAGAAGACCAGGAAACTGGAGACTCTTTTCAAACTGCATATGTCATTTCTGGGCTGTTGCTAGTTCCCTTATGTGTGGCGCTGAGTGTTCTTTTAGGTTGGCATATCTACCTCACTTTGCAAAACAAGACCACAATTGAGTACCATGAAGGAGTGAGAGGTATGTGGCTTGCAGAGAAAGGAGGGCAAATATATTCCCATCCATATGATCGTGGTGCCTATGAAAATTTGACAACGGTACTGGGACCAAATATCTGCAGTTGGGCATTCCCCACATCGGGACATATTGGATCCGGACTTCGCTTTCGTACCGCCTATGATTACCCTTCTGGAGCATCAATGTCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DHHC

Protein Analysis

351

Amino Acids

38.76

Weight (kDa)

7.98

Isoelectric Point (pI)

54.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHC PF01529 160 - 283 4.3e-40 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 432
Acc16I TGCGCA 1 cut(s) 560
AccB1I GGYRCC 3 cut(s) 37, 151, 917
AccII CGCG 2 cut(s) 125, 205
AccIII TCCGGA 1 cut(s) 995
AciI CCGC 6 cut(s) 35, 123, 125, 170, 176, 1014
AclWI GGATC 6 cut(s) 389, 402, 573, 689, 987, 1000
AcoI YGGCCR 2 cut(s) 351, 598
AcsI RAATTY 1 cut(s) 928
AcuI CTGAAG 1 cut(s) 373
AcyI GRCGYC 3 cut(s) 132, 146, 429
AfaI GTAC 4 cut(s) 419, 845, 942, 1012
AfiI CCNNNNNNNGG 5 cut(s) 124, 161, 390, 979, 980
AgsI TTSAA 2 cut(s) 370, 734
AjnI CCWGG 3 cut(s) 160, 663, 711
AleI CACNNNNGTG 1 cut(s) 347
AluBI AGCT 2 cut(s) 104, 518
AluI AGCT 2 cut(s) 104, 518
Alw21I GWGCWC 1 cut(s) 526
Alw26I GTCTC 3 cut(s) 123, 308, 717
Alw44I GTGCAC 1 cut(s) 522
AlwI GGATC 6 cut(s) 389, 402, 573, 689, 987, 1000
Aor13HI TCCGGA 1 cut(s) 995
AoxI GGCC 3 cut(s) 163, 351, 598
ApaLI GTGCAC 1 cut(s) 522
ApoI RAATTY 1 cut(s) 928
ArsI GACNNNNNNTTYG 4 cut(s) 281, 313, 990, 1022
AseI ATTAAT 1 cut(s) 585
AspLEI GCGC 3 cut(s) 468, 561, 784
AspS9I GGNCC 2 cut(s) 164, 947
AsuC2I CCSGG 2 cut(s) 318, 399
AsuHPI GGTGA 3 cut(s) 57, 65, 334
AvaII GGWCC 1 cut(s) 947
BaeGI GKGCMC 1 cut(s) 526
BalI TGGCCA 1 cut(s) 600
BamHI GGATCC 2 cut(s) 394, 992
BanI GGYRCC 3 cut(s) 37, 151, 917
BauI CACGAG 2 cut(s) 455, 519
BbsI GAAGAC 4 cut(s) 268, 358, 379, 714
Bbv12I GWGCWC 1 cut(s) 526
BccI CCATC 2 cut(s) 49, 909
BceAI ACGGC 3 cut(s) 119, 317, 338
BciT130I CCWGG 3 cut(s) 162, 665, 713
BcnI CCSGG 2 cut(s) 318, 399
BcoDI GTCTC 3 cut(s) 123, 308, 717
BfaI CTAG 1 cut(s) 764
BfmI CTRYAG 1 cut(s) 958
BfoI RGCGCY 1 cut(s) 785
BisI GCNGC 1 cut(s) 36
BlsI GCNGC 1 cut(s) 37
Bme1390I CCNGG 5 cut(s) 162, 318, 399, 665, 713
Bme18I GGWCC 1 cut(s) 947
BmgT120I GGNCC 2 cut(s) 164, 947
BmiI GGNNCC 7 cut(s) 39, 153, 396, 408, 919, 948, 994
BmrFI CCNGG 5 cut(s) 162, 318, 399, 665, 713
BmrI ACTGGG 1 cut(s) 953
BmsI GCATC 1 cut(s) 1046
BmuI ACTGGG 1 cut(s) 953
BpiI GAAGAC 4 cut(s) 268, 358, 379, 714
BpmI CTGGAG 3 cut(s) 144, 741, 1053
BpuMI CCSGG 2 cut(s) 318, 399
BsaAI YACGTR 1 cut(s) 417
BsaHI GRCGYC 3 cut(s) 132, 146, 429
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 4 cut(s) 123, 211, 270, 663
BsaWI WCCGGW 1 cut(s) 995
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bsc4I CCNNNNNNNGG 5 cut(s) 124, 161, 390, 979, 980
Bse118I RCCGGY 3 cut(s) 31, 148, 344
Bse1I ACTGG 2 cut(s) 724, 948
Bse3DI GCAATG 1 cut(s) 571
BseAI TCCGGA 1 cut(s) 995
BseBI CCWGG 3 cut(s) 162, 665, 713
BseDI CCNNGG 4 cut(s) 123, 211, 270, 663
BseGI GGATG 1 cut(s) 901
BseLI CCNNNNNNNGG 5 cut(s) 124, 161, 390, 979, 980
BseMI GCAATG 1 cut(s) 571
BseMII CTCAG 1 cut(s) 776
BseNI ACTGG 2 cut(s) 724, 948
BseRI GAGGAG 1 cut(s) 147
BseSI GKGCMC 1 cut(s) 526
Bsh1236I CGCG 2 cut(s) 125, 205
Bsh1285I CGRYCG 1 cut(s) 32
BshFI GGCC 3 cut(s) 165, 353, 600
BshNI GGYRCC 3 cut(s) 37, 151, 917
BsiEI CGRYCG 1 cut(s) 32
BsiHKAI GWGCWC 1 cut(s) 526
BsiSI CCGG 7 cut(s) 32, 149, 179, 317, 345, 398, 996
BslFI GGGAC 2 cut(s) 960, 996
BslI CCNNNNNNNGG 5 cut(s) 124, 161, 390, 979, 980
BsmAI GTCTC 3 cut(s) 123, 308, 717
BsmBI CGTCTC 1 cut(s) 123
BsmFI GGGAC 2 cut(s) 960, 996
BsmI GAATGC 2 cut(s) 93, 968
BsnI GGCC 3 cut(s) 165, 353, 600
Bso31I GGTCTC 1 cut(s) 308
Bsp1286I GDGCHC 1 cut(s) 526
Bsp13I TCCGGA 1 cut(s) 995
Bsp143I GATC 6 cut(s) 394, 459, 565, 694, 910, 992
BspACI CCGC 6 cut(s) 35, 123, 125, 170, 176, 1014
BspANI GGCC 3 cut(s) 165, 353, 600
BspCNI CTCAG 1 cut(s) 777
BspEI TCCGGA 1 cut(s) 995
BspFNI CGCG 2 cut(s) 125, 205
BspLI GGNNCC 7 cut(s) 39, 153, 396, 408, 919, 948, 994
BspMAI CTGCAG 1 cut(s) 962
BspPI GGATC 6 cut(s) 389, 402, 573, 689, 987, 1000
BspT107I GGYRCC 3 cut(s) 37, 151, 917
BspTNI GGTCTC 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 571
BsrFI RCCGGY 3 cut(s) 31, 148, 344
BsrI ACTGG 2 cut(s) 724, 948
BssAI RCCGGY 3 cut(s) 31, 148, 344
BssECI CCNNGG 4 cut(s) 123, 211, 270, 663
BssMI GATC 6 cut(s) 394, 459, 565, 694, 910, 992
BssNI GRCGYC 3 cut(s) 132, 146, 429
BssSI CACGAG 2 cut(s) 455, 519
Bst2BI CACGAG 2 cut(s) 455, 519
Bst2UI CCWGG 3 cut(s) 162, 665, 713
Bst4CI ACNGT 4 cut(s) 235, 274, 293, 940
BstACI GRCGYC 3 cut(s) 132, 146, 429
BstBAI YACGTR 1 cut(s) 417
BstC8I GCNNGC 4 cut(s) 102, 516, 652, 873
BstDEI CTNAG 1 cut(s) 785
BstDSI CCRYGG 2 cut(s) 123, 270
BstEII GGTNACC 2 cut(s) 63, 443
BstENI CCTNNNNNAGG 1 cut(s) 159
BstF5I GGATG 1 cut(s) 901
BstFNI CGCG 2 cut(s) 125, 205
BstH2I RGCGCY 1 cut(s) 785
BstHHI GCGC 3 cut(s) 468, 561, 784
BstKTI GATC 6 cut(s) 397, 462, 568, 697, 913, 995
BstMAI GTCTC 3 cut(s) 123, 308, 717
BstMBI GATC 6 cut(s) 394, 459, 565, 694, 910, 992
BstMCI CGRYCG 1 cut(s) 32
BstMWI GCNNNNNNNGC 2 cut(s) 131, 647
BstNI CCWGG 3 cut(s) 162, 665, 713
BstNSI RCATGY 2 cut(s) 424, 654
BstPI GGTNACC 2 cut(s) 63, 443
BstSCI CCNGG 5 cut(s) 160, 316, 397, 663, 711
BstSFI CTRYAG 1 cut(s) 958
BstSLI GKGCMC 1 cut(s) 526
BstUI CGCG 2 cut(s) 125, 205
BstV2I GAAGAC 4 cut(s) 268, 358, 379, 714
BstX2I RGATCY 2 cut(s) 394, 992
BstXI CCANNNNNNTGG 1 cut(s) 719
BstYI RGATCY 2 cut(s) 394, 992
BsuRI GGCC 3 cut(s) 165, 353, 600
BtgI CCRYGG 2 cut(s) 123, 270
BtsCI GGATG 1 cut(s) 901
Cac8I GCNNGC 4 cut(s) 102, 516, 652, 873
CfoI GCGC 3 cut(s) 468, 561, 784
Cfr10I RCCGGY 3 cut(s) 31, 148, 344
Cfr13I GGNCC 2 cut(s) 164, 947
Cfr42I CCGCGG 1 cut(s) 126
CseI GACGC 4 cut(s) 140, 154, 211, 352
Csp6I GTAC 4 cut(s) 418, 844, 941, 1011
CviAII CATG 7 cut(s) 307, 358, 421, 562, 602, 651, 848
CviQI GTAC 4 cut(s) 418, 844, 941, 1011
DdeI CTNAG 1 cut(s) 785
DpnI GATC 6 cut(s) 396, 461, 567, 696, 912, 994
DpnII GATC 6 cut(s) 394, 459, 565, 694, 910, 992
EaeI YGGCCR 2 cut(s) 351, 598
EciI GGCGGA 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 308
Eco47I GGWCC 1 cut(s) 947
Eco57I CTGAAG 1 cut(s) 373
Eco91I GGTNACC 2 cut(s) 63, 443
EcoNI CCTNNNNNAGG 1 cut(s) 159
EcoO109I RGGNCCY 1 cut(s) 164
EcoO65I GGTNACC 2 cut(s) 63, 443
EcoRII CCWGG 3 cut(s) 160, 663, 711
Esp3I CGTCTC 1 cut(s) 123
FaeI CATG 7 cut(s) 310, 361, 424, 565, 605, 654, 851
FaqI GGGAC 2 cut(s) 960, 996
FatI CATG 7 cut(s) 306, 357, 420, 561, 601, 650, 847
FauI CCCGC 2 cut(s) 118, 130
FauNDI CATATG 3 cut(s) 578, 742, 907
Fnu4HI GCNGC 1 cut(s) 36
FokI GGATG 1 cut(s) 888
Fsp4HI GCNGC 1 cut(s) 36
FspBI CTAG 1 cut(s) 764
FspI TGCGCA 1 cut(s) 560
GlaI GCGC 3 cut(s) 467, 560, 783
GluI GCNGC 1 cut(s) 36
GsuI CTGGAG 3 cut(s) 144, 741, 1053
HaeII RGCGCY 1 cut(s) 785
HaeIII GGCC 3 cut(s) 165, 353, 600
HapII CCGG 7 cut(s) 32, 149, 179, 317, 345, 398, 996
HgaI GACGC 4 cut(s) 140, 154, 211, 352
HhaI GCGC 3 cut(s) 468, 561, 784
Hin1I GRCGYC 3 cut(s) 132, 146, 429
Hin1II CATG 7 cut(s) 310, 361, 424, 565, 605, 654, 851
Hin6I GCGC 3 cut(s) 466, 559, 782
HinP1I GCGC 3 cut(s) 466, 559, 782
HinfI GANTC 3 cut(s) 70, 437, 725
HpaII CCGG 7 cut(s) 32, 149, 179, 317, 345, 398, 996
HphI GGTGA 3 cut(s) 57, 65, 334
Hpy166II GTNNAC 1 cut(s) 524
Hpy188I TCNGA 2 cut(s) 24, 1048
Hpy188III TCNNGA 5 cut(s) 191, 698, 981, 996, 1032
Hpy8I GTNNAC 1 cut(s) 524
Hpy99I CGWCG 8 cut(s) 140, 239, 242, 316, 338, 431, 434, 680
HpyAV CCTTC 2 cut(s) 845, 1038
HpyCH4III ACNGT 4 cut(s) 235, 274, 293, 940
HpyCH4IV ACGT 2 cut(s) 416, 429
HpyCH4V TGCA 9 cut(s) 55, 524, 543, 576, 605, 740, 823, 875, 960
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 647
HpyF3I CTNAG 1 cut(s) 785
HpySE526I ACGT 2 cut(s) 416, 429
Hsp92I GRCGYC 3 cut(s) 132, 146, 429
Hsp92II CATG 7 cut(s) 310, 361, 424, 565, 605, 654, 851
HspAI GCGC 3 cut(s) 466, 559, 782
Kpn2I TCCGGA 1 cut(s) 995
KspI CCGCGG 1 cut(s) 126
Kzo9I GATC 6 cut(s) 394, 459, 565, 694, 910, 992
LmnI GCTCC 1 cut(s) 1034
LweI GCATC 1 cut(s) 1046
MaeI CTAG 1 cut(s) 764
MaeII ACGT 2 cut(s) 416, 429
MaeIII GTNAC 3 cut(s) 63, 229, 443
MalI GATC 6 cut(s) 396, 461, 567, 696, 912, 994
MboI GATC 6 cut(s) 394, 459, 565, 694, 910, 992
MboII GAAGA 5 cut(s) 268, 358, 379, 446, 719
MfeI CAATTG 1 cut(s) 837
MflI RGATCY 2 cut(s) 394, 992
MhlI GDGCHC 1 cut(s) 526
MlsI TGGCCA 1 cut(s) 600
MluCI AATT 3 cut(s) 370, 837, 928
MluNI TGGCCA 1 cut(s) 600
MlyI GAGTC 2 cut(s) 64, 719
Mox20I TGGCCA 1 cut(s) 600
MroI TCCGGA 1 cut(s) 995
MscI TGGCCA 1 cut(s) 600
MseI TTAA 3 cut(s) 17, 482, 585
MslI CAYNNNNRTG 4 cut(s) 347, 419, 534, 906
Msp20I TGGCCA 1 cut(s) 600
MspA1I CMGCKG 2 cut(s) 104, 125
MspI CCGG 7 cut(s) 32, 149, 179, 317, 345, 398, 996
MspR9I CCNGG 5 cut(s) 162, 318, 399, 665, 713
MunI CAATTG 1 cut(s) 837
Mva1269I GAATGC 2 cut(s) 93, 968
MvaI CCWGG 3 cut(s) 162, 665, 713
MvnI CGCG 2 cut(s) 125, 205
MwoI GCNNNNNNNGC 2 cut(s) 131, 647
NciI CCSGG 2 cut(s) 318, 399
NdeI CATATG 3 cut(s) 578, 742, 907
NdeII GATC 6 cut(s) 394, 459, 565, 694, 910, 992
NlaIII CATG 7 cut(s) 310, 361, 424, 565, 605, 654, 851
NlaIV GGNNCC 7 cut(s) 39, 153, 396, 408, 919, 948, 994
NmeAIII GCCGAG 2 cut(s) 192, 356
NmuCI GTSAC 1 cut(s) 63
NsbI TGCGCA 1 cut(s) 560
NspI RCATGY 2 cut(s) 424, 654
OliI CACNNNNGTG 1 cut(s) 347
PaeI GCATGC 1 cut(s) 654
PctI GAATGC 2 cut(s) 93, 968
PfeI GAWTC 1 cut(s) 437
PflFI GACNNNGTC 1 cut(s) 134
PkrI GCNGC 1 cut(s) 37
PleI GAGTC 2 cut(s) 64, 719
PpsI GAGTC 2 cut(s) 64, 719
Ppu21I YACGTR 1 cut(s) 417
PshBI ATTAAT 1 cut(s) 585
Psp6I CCWGG 3 cut(s) 160, 663, 711
PspEI GGTNACC 2 cut(s) 63, 443
PspGI CCWGG 3 cut(s) 160, 663, 711
PspN4I GGNNCC 7 cut(s) 39, 153, 396, 408, 919, 948, 994
PspPI GGNCC 2 cut(s) 164, 947
PstI CTGCAG 1 cut(s) 962
PsuI RGATCY 2 cut(s) 394, 992
PsyI GACNNNGTC 1 cut(s) 134
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 4 cut(s) 419, 845, 942, 1012
RsaNI GTAC 4 cut(s) 418, 844, 941, 1011
RseI CAYNNNNRTG 4 cut(s) 347, 419, 534, 906
SacII CCGCGG 1 cut(s) 126
SaqAI TTAA 3 cut(s) 17, 482, 585
SatI GCNGC 1 cut(s) 36
Sau3AI GATC 6 cut(s) 394, 459, 565, 694, 910, 992
Sau96I GGNCC 2 cut(s) 164, 947
SchI GAGTC 2 cut(s) 64, 719
ScrFI CCNGG 5 cut(s) 162, 318, 399, 665, 713
SduI GDGCHC 1 cut(s) 526
SfaNI GCATC 1 cut(s) 1046
SfcI CTRYAG 1 cut(s) 958
Sfr303I CCGCGG 1 cut(s) 126
SgrAI CRCCGGYG 2 cut(s) 148, 344
SgrBI CCGCGG 1 cut(s) 126
SinI GGWCC 1 cut(s) 947
SmiMI CAYNNNNRTG 4 cut(s) 347, 419, 534, 906
SphI GCATGC 1 cut(s) 654
Sse9I AATT 3 cut(s) 370, 837, 928
SsiI CCGC 6 cut(s) 35, 123, 125, 170, 176, 1014
SspI AATATT 1 cut(s) 187
SspMI CTAG 1 cut(s) 764
StyD4I CCNGG 5 cut(s) 160, 316, 397, 663, 711
TaaI ACNGT 4 cut(s) 235, 274, 293, 940
TaiI ACGT 2 cut(s) 419, 432
TaqI TCGA 1 cut(s) 432
TaqII GACCGA 2 cut(s) 51, 290
TasI AATT 3 cut(s) 370, 837, 928
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 1 cut(s) 437
Tru1I TTAA 3 cut(s) 17, 482, 585
Tru9I TTAA 3 cut(s) 17, 482, 585
TseFI GTSAC 1 cut(s) 63
Tsp45I GTSAC 1 cut(s) 63
TspDTI ATGAA 7 cut(s) 17, 268, 274, 341, 613, 864, 939
TspGWI ACGGA 1 cut(s) 373
Tth111I GACNNNGTC 1 cut(s) 134
VneI GTGCAC 1 cut(s) 522
VpaK11BI GGWCC 1 cut(s) 947
VspI ATTAAT 1 cut(s) 585
XagI CCTNNNNNAGG 1 cut(s) 159
XapI RAATTY 1 cut(s) 928
XceI RCATGY 2 cut(s) 424, 654
XspI CTAG 1 cut(s) 764
ZraI GACGTC 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.