Rorug03G0080700

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000610
Physical Location & Seq
Reverse (-)
6301118 .. 6304684
3567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0080700.1

Sequence Viewer

Length: 1095 bp
ATGGCAATTGGTTATGGTAATAGCTATATGCCATTTGTGGCCATGGTGTTTGTGCAAGTGTGCTATGCAGGAATGAACATCACTTCAAAGCTTGCTCTGGAGGCGGAGCTGAACCCTCTTATTCTTGTAGCTTATCGACAAATTTTCGCCACCGTAGCCTTAGCTCCTTTCGCTTATTGGATGGAATGGAAAACAAGACCCAAGATCACAATGCCTATCCTTTTCCAGATTTTCCTATGTTCCTTAACTGGAGCAACTGCAAACCAAGTTTTCTATTTTCTTGGATTGAACAAAACCACTCCAACCGTTGCTTGTGCATTGACAAATACGCTCCCAGCTATGACATTTATCCTTGCAGTCCTTTTCAGACAAGAATCAGCAAAAATGAAGAGCAAGGCAGGGTTGGCTAAGGTGATGGGAACAGCAGTGTGTGTATGTGGAGCCATGATACTCTCATTTTACCATGGAAAAAACATTGGTATCGGTGAATCCAAAATTCACTGGAGTTATGCTCAGAGAATGGGACAGTCAAACTCAGGAAACAATGCCAACTCCTTTGTTGGCCCCCTTTTAGTCATCATCAGCACCTTAGGTTGGGCAATGTGGTTCATCATCCAAGCAACAGTGAGTGATAGGTTTCCAGCTCCCTACACAAGCACTACATTGATGTGTTTCATGGCCAGTTTGGAGTGTGGGCTCATTGCTGTAATTGTCGAGCATAATGGACGTGCATGGTCATTGACCAATCCCATGAAGCTTACTGCGAGCCTCTATTCAGGGATTATGGGTTCTGCAGTAGCATTTTTCCTCACTTCATGGAGCATTCAGAGGAAAGGAGCTCTTTATGCGTCAGTGTTCAGCCCTTTGCTGCTCATTGTTGTGGCTGTTTCGAGTTGGGCTCTGCTTGATGAGAAATTATACCTGGGAACTGCTTTAGGATCGATCTTGATTGTGGCCGGGCTCTATTCTGTTCTTTGGGGGAAGACCAAGGAGACCGCTAAGAATGAGGGCAAAGAAACAGACGCAAATACAAAGCCAAATGCTGCTGCCAAGGAAATTGAGAAAAACGACTTGGAGCTGCAGCTGCCCAAGTGA

Protein Analysis

364

Amino Acids

39.64

Weight (kDa)

9.2

Isoelectric Point (pI)

31.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 14 - 151 1.7e-16 EamA-like transporter family
EamA PF00892 188 - 325 1.3e-14 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000610)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60020 AT5G60020 AT5G60020
fragaria_vesca FvH4_2g09062 FvH4_2g09062 FvH4_3g05920 FvH4_3g34100 FvH4_3g34310 FvH4_3g34340 FvH4_6g13390
malus_domestica MD00G1015000.v1.1 MD03G1106500.v1.1 MD03G1106600.v1.1 MD04G1131500.v1.1 MD11G1118900.v1.1 MD11G1119000.v1.1 MD12G1144600.v1.1
prunus_persica Prupe.6G089100_v2.0.a1 Prupe.6G089300_v2.0.a1 Prupe.6G258000_v2.0.a1
pyrus_communis pycom03g08590 pycom10g02940 pycom11g09990 pycom11g10060
rosa_chinensis RchiOBHm_Chr2g0154491 RchiOBHm_Chr3g0466271 RchiOBHm_Chr5g0061891 RchiOBHm_Chr5g0061911 RchiOBHm_Chr5g0061951 RchiOBHm_Chr5g0061961 RchiOBHm_Chr6g0264371
rosa_laevigata RLG00000024591 RLG00000035514 RLG00000035516 RLG00000035518 RLG00000035519
rosa_multiflora Rmu_sc0003480.1_g000021 Rmu_sc0011641.1_g000002 Rmu_sc0011641.1_g000009
rosa_roxburghii Rroxscaffold_1G00018790 Rroxscaffold_1G00018820 Rroxscaffold_1G00018850 Rroxscaffold_2G00122520 Rroxscaffold_2G00122540 Rroxscaffold_6G00414660
rosa_rugosa Rorug03G0080700 Rorug04G0180900 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345100 Rorug05G0345200 Rorug06G0011800
rosa_samantha Rh3DG148900 Rh5AG405700 Rh5AG406100 Rh5AG406200 Rh5BG419200 Rh5BG419400 Rh5CG443100 Rh5CG443300 Rh5CG443500 Rh5CG443600 Rh5DG432700 Rh5DG434900 Rh6BG128700 Rh6CG127600 Rh6DG114200 Rh6DG114300
rosa_wichuraiana Rw3G011980 Rw5G038100 Rw5G038140 Rw5G038160 Rw5G038200 Rw5G038210 Rw5G038250 Rw5G038270 Rw5G038290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 104, 996
AclWI GGATC 1 cut(s) 946
AcoI YGGCCR 3 cut(s) 39, 678, 954
AcsI RAATTY 2 cut(s) 141, 495
AfiI CCNNNNNNNGG 1 cut(s) 594
AgsI TTSAA 2 cut(s) 87, 289
AjiI CACGTC 1 cut(s) 728
AjnI CCWGG 1 cut(s) 921
AjuI GAANNNNNNNTTGG 2 cut(s) 1055, 1087
AloI GAACNNNNNNTCC 2 cut(s) 772, 804
Alw21I GWGCWC 1 cut(s) 841
Alw26I GTCTC 1 cut(s) 986
AlwI GGATC 1 cut(s) 946
AoxI GGCC 4 cut(s) 39, 562, 678, 954
ApeKI GCWGC 6 cut(s) 868, 1043, 1046, 1078, 1081, 1084
ApoI RAATTY 2 cut(s) 141, 495
AspS9I GGNCC 1 cut(s) 563
AsuC2I CCSGG 1 cut(s) 958
AsuHPI GGTGA 2 cut(s) 424, 497
AxyI CCTNAGG 1 cut(s) 589
BaeI ACNNNNGTAYC 2 cut(s) 463, 496
BalI TGGCCA 2 cut(s) 41, 680
BanII GRGCYC 4 cut(s) 699, 841, 901, 963
BbsI GAAGAC 1 cut(s) 989
Bbv12I GWGCWC 1 cut(s) 841
BbvI GCAGC 5 cut(s) 855, 1030, 1033, 1065, 1071
BccI CCATC 2 cut(s) 175, 409
BciT130I CCWGG 1 cut(s) 923
BcnI CCSGG 1 cut(s) 958
BcoDI GTCTC 1 cut(s) 986
BfmI CTRYAG 2 cut(s) 792, 1079
BisI GCNGC 6 cut(s) 869, 1044, 1047, 1079, 1082, 1085
BlsI GCNGC 6 cut(s) 870, 1045, 1048, 1080, 1083, 1086
Bme1390I CCNGG 2 cut(s) 923, 958
BmgBI CACGTC 1 cut(s) 728
BmgT120I GGNCC 1 cut(s) 563
BmiI GGNNCC 2 cut(s) 442, 565
BmrFI CCNGG 2 cut(s) 923, 958
BpiI GAAGAC 1 cut(s) 989
BplI GAGNNNNNCTC 4 cut(s) 496, 528, 883, 915
BpmI CTGGAG 3 cut(s) 119, 270, 523
Bpu10I CCTNAGC 2 cut(s) 160, 408
BpuMI CCSGG 1 cut(s) 958
Bsa29I ATCGAT 1 cut(s) 941
BsaI GGTCTC 1 cut(s) 986
BsaJI CCNNGG 5 cut(s) 42, 463, 922, 987, 1050
Bsc4I CCNNNNNNNGG 1 cut(s) 594
Bse1I ACTGG 3 cut(s) 253, 506, 681
Bse21I CCTNAGG 1 cut(s) 589
Bse3DI GCAATG 2 cut(s) 606, 699
BseBI CCWGG 1 cut(s) 923
BseCI ATCGAT 1 cut(s) 941
BseDI CCNNGG 5 cut(s) 42, 463, 922, 987, 1050
BseGI GGATG 2 cut(s) 186, 612
BseLI CCNNNNNNNGG 1 cut(s) 594
BseMI GCAATG 2 cut(s) 606, 699
BseMII CTCAG 2 cut(s) 527, 549
BseNI ACTGG 3 cut(s) 253, 506, 681
BseXI GCAGC 5 cut(s) 855, 1030, 1033, 1065, 1071
BseYI CCCAGC 1 cut(s) 334
BshFI GGCC 4 cut(s) 41, 564, 680, 956
BshVI ATCGAT 1 cut(s) 941
BsiHKAI GWGCWC 1 cut(s) 841
BsiSI CCGG 1 cut(s) 957
BslFI GGGAC 1 cut(s) 537
BslI CCNNNNNNNGG 1 cut(s) 594
BsmAI GTCTC 1 cut(s) 986
BsmFI GGGAC 1 cut(s) 537
BsmI GAATGC 1 cut(s) 822
BsnI GGCC 4 cut(s) 41, 564, 680, 956
Bso31I GGTCTC 1 cut(s) 986
Bsp1286I GDGCHC 4 cut(s) 699, 841, 901, 963
Bsp143I GATC 3 cut(s) 204, 938, 942
Bsp19I CCATGG 2 cut(s) 42, 463
BspACI CCGC 2 cut(s) 104, 996
BspANI GGCC 4 cut(s) 41, 564, 680, 956
BspCNI CTCAG 2 cut(s) 526, 548
BspDI ATCGAT 1 cut(s) 941
BspLI GGNNCC 2 cut(s) 442, 565
BspMAI CTGCAG 2 cut(s) 796, 1083
BspPI GGATC 1 cut(s) 946
BspQI GCTCTTC 1 cut(s) 383
BspTNI GGTCTC 1 cut(s) 986
BsrDI GCAATG 2 cut(s) 606, 699
BsrI ACTGG 3 cut(s) 253, 506, 681
BssECI CCNNGG 5 cut(s) 42, 463, 922, 987, 1050
BssMI GATC 3 cut(s) 204, 938, 942
BssT1I CCWWGG 4 cut(s) 42, 463, 987, 1050
Bst2UI CCWGG 1 cut(s) 923
Bst4CI ACNGT 4 cut(s) 154, 307, 528, 625
Bst6I CTCTTC 1 cut(s) 383
BstC8I GCNNGC 2 cut(s) 93, 766
BstDEI CTNAG 6 cut(s) 160, 408, 513, 535, 589, 999
BstDSI CCRYGG 2 cut(s) 42, 463
BstF5I GGATG 2 cut(s) 186, 612
BstKTI GATC 3 cut(s) 207, 941, 945
BstMAI GTCTC 1 cut(s) 986
BstMBI GATC 3 cut(s) 204, 938, 942
BstMWI GCNNNNNNNGC 6 cut(s) 101, 155, 170, 404, 845, 1084
BstNI CCWGG 1 cut(s) 923
BstSCI CCNGG 2 cut(s) 921, 956
BstSFI CTRYAG 2 cut(s) 792, 1079
BstV1I GCAGC 5 cut(s) 855, 1030, 1033, 1065, 1071
BstV2I GAAGAC 1 cut(s) 989
Bsu15I ATCGAT 1 cut(s) 941
Bsu36I CCTNAGG 1 cut(s) 589
BsuRI GGCC 4 cut(s) 41, 564, 680, 956
BsuTUI ATCGAT 1 cut(s) 941
BtgI CCRYGG 2 cut(s) 42, 463
BtrI CACGTC 1 cut(s) 728
BtsCI GGATG 2 cut(s) 186, 612
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 4 cut(s) 432, 499, 630, 858
Cac8I GCNNGC 2 cut(s) 93, 766
Cfr13I GGNCC 1 cut(s) 563
ClaI ATCGAT 1 cut(s) 941
CseI GACGC 2 cut(s) 837, 1031
CviAII CATG 7 cut(s) 43, 445, 464, 676, 732, 751, 816
DdeI CTNAG 6 cut(s) 160, 408, 513, 535, 589, 999
DpnI GATC 3 cut(s) 206, 940, 944
DpnII GATC 3 cut(s) 204, 938, 942
EaeI YGGCCR 3 cut(s) 39, 678, 954
Eam1104I CTCTTC 1 cut(s) 383
EarI CTCTTC 1 cut(s) 383
EciI GGCGGA 1 cut(s) 119
Ecl136II GAGCTC 1 cut(s) 839
Eco130I CCWWGG 4 cut(s) 42, 463, 987, 1050
Eco24I GRGCYC 4 cut(s) 699, 841, 901, 963
Eco31I GGTCTC 1 cut(s) 986
Eco53kI GAGCTC 1 cut(s) 839
Eco81I CCTNAGG 1 cut(s) 589
EcoICRI GAGCTC 1 cut(s) 839
EcoRII CCWGG 1 cut(s) 921
EcoT14I CCWWGG 4 cut(s) 42, 463, 987, 1050
EcoT38I GRGCYC 4 cut(s) 699, 841, 901, 963
ErhI CCWWGG 4 cut(s) 42, 463, 987, 1050
FaeI CATG 7 cut(s) 46, 448, 467, 679, 735, 754, 819
FalI AAGNNNNNCTT 2 cut(s) 825, 857
FaqI GGGAC 1 cut(s) 537
FatI CATG 7 cut(s) 42, 444, 463, 675, 731, 750, 815
Fnu4HI GCNGC 6 cut(s) 869, 1044, 1047, 1079, 1082, 1085
FokI GGATG 2 cut(s) 193, 599
FriOI GRGCYC 4 cut(s) 699, 841, 901, 963
Fsp4HI GCNGC 6 cut(s) 869, 1044, 1047, 1079, 1082, 1085
GluI GCNGC 6 cut(s) 869, 1044, 1047, 1079, 1082, 1085
GsaI CCCAGC 1 cut(s) 338
GsuI CTGGAG 3 cut(s) 119, 270, 523
HaeIII GGCC 4 cut(s) 41, 564, 680, 956
HapII CCGG 1 cut(s) 957
HgaI GACGC 2 cut(s) 837, 1031
Hin1II CATG 7 cut(s) 46, 448, 467, 679, 735, 754, 819
HindIII AAGCTT 2 cut(s) 89, 755
HinfI GANTC 2 cut(s) 374, 488
HpaII CCGG 1 cut(s) 957
HphI GGTGA 2 cut(s) 424, 497
Hpy188I TCNGA 3 cut(s) 368, 516, 828
Hpy188III TCNNGA 4 cut(s) 98, 226, 537, 946
HpyCH4III ACNGT 4 cut(s) 154, 307, 528, 625
HpyCH4IV ACGT 1 cut(s) 727
HpyCH4V TGCA 8 cut(s) 55, 68, 260, 317, 356, 731, 794, 1081
HpyF10VI GCNNNNNNNGC 6 cut(s) 101, 155, 170, 404, 845, 1084
HpyF3I CTNAG 6 cut(s) 160, 408, 513, 535, 589, 999
HpySE526I ACGT 1 cut(s) 727
Hsp92II CATG 7 cut(s) 46, 448, 467, 679, 735, 754, 819
Kzo9I GATC 3 cut(s) 204, 938, 942
LguI GCTCTTC 1 cut(s) 383
LmnI GCTCC 9 cut(s) 106, 169, 251, 336, 440, 649, 819, 836, 1075
Lsp1109I GCAGC 5 cut(s) 855, 1030, 1033, 1065, 1071
MaeII ACGT 1 cut(s) 727
MalI GATC 3 cut(s) 206, 940, 944
MboI GATC 3 cut(s) 204, 938, 942
MboII GAAGA 2 cut(s) 400, 994
MfeI CAATTG 1 cut(s) 6
MhlI GDGCHC 4 cut(s) 699, 841, 901, 963
MlsI TGGCCA 2 cut(s) 41, 680
MluCI AATT 6 cut(s) 6, 141, 495, 708, 914, 1056
MluNI TGGCCA 2 cut(s) 41, 680
MmeI TCCRAC 1 cut(s) 326
MnlI CCTC 6 cut(s) 94, 126, 779, 818, 822, 1000
Mox20I TGGCCA 2 cut(s) 41, 680
MscI TGGCCA 2 cut(s) 41, 680
MseI TTAA 1 cut(s) 245
MslI CAYNNNNRTG 2 cut(s) 667, 878
Msp20I TGGCCA 2 cut(s) 41, 680
MspA1I CMGCKG 1 cut(s) 1084
MspI CCGG 1 cut(s) 957
MspR9I CCNGG 2 cut(s) 923, 958
MunI CAATTG 1 cut(s) 6
Mva1269I GAATGC 1 cut(s) 822
MvaI CCWGG 1 cut(s) 923
MwoI GCNNNNNNNGC 6 cut(s) 101, 155, 170, 404, 845, 1084
NciI CCSGG 1 cut(s) 958
NcoI CCATGG 2 cut(s) 42, 463
NdeII GATC 3 cut(s) 204, 938, 942
NlaIII CATG 7 cut(s) 46, 448, 467, 679, 735, 754, 819
NlaIV GGNNCC 2 cut(s) 442, 565
PciSI GCTCTTC 1 cut(s) 383
PctI GAATGC 1 cut(s) 822
PfeI GAWTC 2 cut(s) 374, 488
PkrI GCNGC 6 cut(s) 870, 1045, 1048, 1080, 1083, 1086
Psp124BI GAGCTC 1 cut(s) 841
Psp6I CCWGG 1 cut(s) 921
PspFI CCCAGC 1 cut(s) 334
PspGI CCWGG 1 cut(s) 921
PspN4I GGNNCC 2 cut(s) 442, 565
PspPI GGNCC 1 cut(s) 563
PstI CTGCAG 2 cut(s) 796, 1083
PvuII CAGCTG 1 cut(s) 1084
RseI CAYNNNNRTG 2 cut(s) 667, 878
SacI GAGCTC 1 cut(s) 841
SapI GCTCTTC 1 cut(s) 383
SaqAI TTAA 1 cut(s) 245
SatI GCNGC 6 cut(s) 869, 1044, 1047, 1079, 1082, 1085
Sau3AI GATC 3 cut(s) 204, 938, 942
Sau96I GGNCC 1 cut(s) 563
ScrFI CCNGG 2 cut(s) 923, 958
SduI GDGCHC 4 cut(s) 699, 841, 901, 963
SfcI CTRYAG 2 cut(s) 792, 1079
SmiMI CAYNNNNRTG 2 cut(s) 667, 878
Sse9I AATT 6 cut(s) 6, 141, 495, 708, 914, 1056
SsiI CCGC 2 cut(s) 104, 996
SstI GAGCTC 1 cut(s) 841
StyD4I CCNGG 2 cut(s) 921, 956
StyI CCWWGG 4 cut(s) 42, 463, 987, 1050
TaaI ACNGT 4 cut(s) 154, 307, 528, 625
TaiI ACGT 1 cut(s) 730
TaqI TCGA 4 cut(s) 136, 714, 890, 941
TasI AATT 6 cut(s) 6, 141, 495, 708, 914, 1056
TfiI GAWTC 2 cut(s) 374, 488
Tru1I TTAA 1 cut(s) 245
Tru9I TTAA 1 cut(s) 245
TscAI CASTG 4 cut(s) 432, 506, 630, 858
TseI GCWGC 6 cut(s) 868, 1043, 1046, 1078, 1081, 1084
TspDTI ATGAA 6 cut(s) 89, 401, 598, 664, 767, 804
TspRI CASTG 4 cut(s) 432, 506, 630, 858
XapI RAATTY 2 cut(s) 141, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.