Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0000610

Physical Location & Seq

Genomic Coordinates Reverse (-)

70238498 .. 70248162

Gene Structure 9665 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG405700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1746 bp

ATGGGCGTTGCTCTTCTTTCAGTTGTTAAGCTCTTTGTTTTCTCTTTCGTTACATTGGGTCTCATTCCTCAGCAGACTCTTGCAAGCATAACGAGACATTACAAGTTTGATATCCAAATGAAAAATGTGACAAGATTGTGTCACACGAAGAGCATTGTAACTGTAAACGGTCAATTTCCTGGGCCTCGAGTTGTTGCTAGGGAGGGTGATCGCCTCCTCATTAGAGTGGTTAACCATGTTCAAAGCAACATCTCCATCCACTGGCATGGGATTCGACAACTTCAAACTGGATGGGCAGATGGTCCAGCATACATTACCCAATGTCCAATACAATCTGGCAACGGTTATGTGTACAACTTCACCATTATAGGCCAAAGAGGCACTCTTTGGTGGCATGCTCACATTTCATGGCTAAGATCAACCATCTATGGTCCCCTAATCATTCTTCCCAAGCGTGGTGTTCCTTATCCATTTACCAAACCCTACAAGGAAGTTCCCATCATCTTTGGAGAATGGTGGAATGCAGATCCTGAAGCAGTCATAAACCAGGCCTTGCAAACTGGGGGAGGACCAAATGTTTCTGATGCTTACACCATCAATGGACTCCCAGGGCCATTGTATAACTGCTCTGCCAAAGATACTTTCAAGCTAAAGGTTCAAGCTGGGAAGACATACCTTCTGCGCCTAATCAATGCAGCGCTGAACGACGAGCTCTTCTTCAGCATTGCAAACCACACCCTCAAAGTGGTGGAAACTGATGCAGTTTACGTCAAACCCTTCGAGACCAACACCATTCTCATTGCCCCGGGACAAACCACAAATGTTCTTCTCAAAACAAAACCCAACTACCCAAACGCCACATTTTTCATGACTGCAAGGCCATACGTGACTGGTCTAGGCACCTTTGATAACTCCACTCTTGCTGGCATTTTAGAATACGAAACGACTACACTTCATTCAACCCTCCCAATGAACAAACTTCCACTCCACAAACCAATTCTCCCAGTTCTAAATGACACAACATTTGCAACAAAATTCTCCAATAAACTCCGTAGCTTGAACACTCCCCAGTTCCCTGCTAATGTTCCACAGAAAGTTGACAAGCATTTGTTCTTCACCATAGGGCTTGGAACCAACCCATGTAGCCAACAGAATCACAACAACCAGACTTGCCAAGGACCCAATGGAACCATGTTTGCTGCTTCAATCAACAATATTTCTTTCACTATGCCAAACACCGCCTTACTCCAATCCCACTTCTTTGGCCAATCCAATGGGGTTTATTCCCCTAATTTTCCAATCAGTCCTTTAATTCCATTTAATTACACTGGAGCCCAGCTGAACAATACTAATGTGGGCAATGGTACTAAGCTAATGGTGCTTCCCTTTAACACTAGTGTGGAGTTGGTGTTGCAGGACACTAGTATTCTTGGTGCTGAAAGTCATCCTCTTCATCTTCATGGCTTTAATTTCTTTGTTGTTGGTCAAGGTTTTGACAACTTCGACCAAAATAAGGACCCTAGGAAGTTCAATCTGATTGACCCTGTTGAGAGAAACACCGTGGGTGTGCCCTCCGGCGGGTGGGTTGCTATTCGGTTTCTAGCAGATAACCCAGGTGTATGGTTCATGCATTGCCACCTTGAGATCCATACTAGCTGGGGCCTGAAGATGGCTTGGGTAGTCTTAGATGGAAAGCTTTCCAATCAAAAGTTGCTTCCTCCGCCACTGGATCTGCCCAAATGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006725 GO:0008150 GO:0008152 GO:0009058 GO:0009698 GO:0009699 GO:0009808 GO:0009809 GO:0009987 GO:0016491 GO:0016722 GO:0019438 GO:0019748 GO:0044237 GO:0044249 GO:0044550 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

581

Amino Acids

64.53

Weight (kDa)

9.24

Isoelectric Point (pI)

30.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu-oxidase_3	PF07732	38 - 151	1.8e-41	Multicopper oxidase
Cu-oxidase	PF00394	164 - 314	8.2e-44	Multicopper oxidase
Cu-oxidase_2	PF07731	439 - 563	1.3e-40	Multicopper oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000610)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60020 AT5G60020 AT5G60020
fragaria_vesca	FvH4_2g09062 FvH4_2g09062 FvH4_3g05920 FvH4_3g34100 FvH4_3g34310 FvH4_3g34340 FvH4_6g13390
malus_domestica	MD00G1015000.v1.1 MD03G1106500.v1.1 MD03G1106600.v1.1 MD04G1131500.v1.1 MD11G1118900.v1.1 MD11G1119000.v1.1 MD12G1144600.v1.1
prunus_persica	Prupe.6G089100_v2.0.a1 Prupe.6G089300_v2.0.a1 Prupe.6G258000_v2.0.a1
pyrus_communis	pycom03g08590 pycom10g02940 pycom11g09990 pycom11g10060
rosa_chinensis	RchiOBHm_Chr2g0154491 RchiOBHm_Chr3g0466271 RchiOBHm_Chr5g0061891 RchiOBHm_Chr5g0061911 RchiOBHm_Chr5g0061951 RchiOBHm_Chr5g0061961 RchiOBHm_Chr6g0264371
rosa_laevigata	RLG00000024591 RLG00000035514 RLG00000035516 RLG00000035518 RLG00000035519
rosa_multiflora	Rmu_sc0003480.1_g000021 Rmu_sc0011641.1_g000002 Rmu_sc0011641.1_g000009
rosa_roxburghii	Rroxscaffold_1G00018790 Rroxscaffold_1G00018820 Rroxscaffold_1G00018850 Rroxscaffold_2G00122520 Rroxscaffold_2G00122540 Rroxscaffold_6G00414660
rosa_rugosa	Rorug03G0080700 Rorug04G0180900 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345100 Rorug05G0345200 Rorug06G0011800
rosa_samantha	Rh3DG148900 Rh5AG405700 Rh5AG406100 Rh5AG406200 Rh5BG419200 Rh5BG419400 Rh5CG443100 Rh5CG443300 Rh5CG443500 Rh5CG443600 Rh5DG432700 Rh5DG434900 Rh6BG128700 Rh6CG127600 Rh6DG114200 Rh6DG114300
rosa_wichuraiana	Rw3G011980 Rw5G038100 Rw5G038140 Rw5G038160 Rw5G038200 Rw5G038210 Rw5G038250 Rw5G038270 Rw5G038290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	899
AccB7I	CCANNNNNTGG	1 cut(s)	261
AciI	CCGC	3 cut(s)	1239, 1578, 1721
AclWI	GGATC	3 cut(s)	521, 1639, 1737
AcoI	YGGCCR	1 cut(s)	1264
AcsI	RAATTY	1 cut(s)	1034
AcuI	CTGAAG	3 cut(s)	552, 703, 1685
AfaI	GTAC	2 cut(s)	353, 1366
AfeI	AGCGCT	1 cut(s)	699
AfiI	CCNNNNNNNGG	8 cut(s)	261, 455, 745, 1513, 1577, 1578, 1581, 1669
AgsI	TTSAA	8 cut(s)	242, 284, 646, 659, 960, 1060, 1206, 1531
AhlI	ACTAGT	2 cut(s)	1394, 1421
AjnI	CCWGG	4 cut(s)	178, 546, 607, 1612
AjuI	GAANNNNNNNTTGG	2 cut(s)	626, 658
AleI	CACNNNNGTG	1 cut(s)	1397
AluBI	AGCT	9 cut(s)	31, 649, 662, 712, 1056, 1339, 1372, 1656, 1696
AluI	AGCT	9 cut(s)	31, 649, 662, 712, 1056, 1339, 1372, 1656, 1696
Alw21I	GWGCWC	1 cut(s)	714
Alw26I	GTCTC	3 cut(s)	65, 88, 776
AlwI	GGATC	3 cut(s)	521, 1639, 1737
AlwNI	CAGNNNCTG	1 cut(s)	530
Ama87I	CYCGRG	2 cut(s)	186, 805
Aor51HI	AGCGCT	1 cut(s)	699
AoxI	GGCC	7 cut(s)	182, 370, 549, 611, 878, 1264, 1660
ApeKI	GCWGC	2 cut(s)	695, 1199
ApoI	RAATTY	1 cut(s)	1034
ArsI	GACNNNNNNTTYG	2 cut(s)	1007, 1039
Asp700I	GAANNNNTTC	1 cut(s)	1696
AspA2I	CCTAGG	1 cut(s)	1520
AspLEI	GCGC	2 cut(s)	684, 700
AspS9I	GGNCC	8 cut(s)	182, 302, 431, 569, 611, 1178, 1516, 1660
AsuC2I	CCSGG	2 cut(s)	806, 807
AsuHPI	GGTGA	3 cut(s)	218, 352, 1108
AvaI	CYCGRG	2 cut(s)	186, 805
AvaII	GGWCC	5 cut(s)	302, 431, 569, 1178, 1516
AvrII	CCTAGG	1 cut(s)	1520
BaeGI	GKGCMC	1 cut(s)	1572
BalI	TGGCCA	1 cut(s)	1266
BanI	GGYRCC	1 cut(s)	899
BanII	GRGCYC	2 cut(s)	714, 1336
BbsI	GAAGAC	1 cut(s)	674
Bbv12I	GWGCWC	1 cut(s)	714
BbvCI	CCTCAGC	1 cut(s)	69
BbvI	GCAGC	2 cut(s)	707, 1186
BccI	CCATC	8 cut(s)	263, 285, 293, 431, 506, 602, 1663, 1682
BcgI	CGANNNNNNTGC	2 cut(s)	254, 288
BciT130I	CCWGG	4 cut(s)	180, 548, 609, 1614
BcnI	CCSGG	2 cut(s)	806, 807
BcoDI	GTCTC	3 cut(s)	65, 88, 776
BcuI	ACTAGT	2 cut(s)	1394, 1421
BfaI	CTAG	7 cut(s)	198, 896, 1395, 1422, 1521, 1601, 1653
BfoI	RGCGCY	1 cut(s)	701
BglI	GCCNNNNNGGC	1 cut(s)	378
BisI	GCNGC	2 cut(s)	696, 1200
BlnI	CCTAGG	1 cut(s)	1520
BlsI	GCNGC	2 cut(s)	697, 1201
Bme1390I	CCNGG	6 cut(s)	180, 548, 609, 806, 807, 1614
Bme18I	GGWCC	5 cut(s)	302, 431, 569, 1178, 1516
BmeT110I	CYCGRG	2 cut(s)	186, 805
BmgT120I	GGNCC	8 cut(s)	182, 302, 431, 569, 611, 1178, 1516, 1660
BmiI	GGNNCC	8 cut(s)	433, 901, 1132, 1180, 1189, 1333, 1518, 1661
BmrFI	CCNGG	6 cut(s)	180, 548, 609, 806, 807, 1614
BmrI	ACTGGG	3 cut(s)	570, 998, 1063
BmsI	GCATC	2 cut(s)	574, 748
BmuI	ACTGGG	3 cut(s)	570, 998, 1063
BpiI	GAAGAC	1 cut(s)	674
BpmI	CTGGAG	1 cut(s)	1350
Bpu10I	CCTNAGC	1 cut(s)	69
BpuEI	CTTGAG	1 cut(s)	1661
BpuMI	CCSGG	2 cut(s)	806, 807
BsaAI	YACGTR	1 cut(s)	886
BsaI	GGTCTC	2 cut(s)	65, 776
BsaJI	CCNNGG	9 cut(s)	179, 607, 608, 804, 805, 1174, 1520, 1560, 1612
BsaXI	ACNNNNNCTCC	6 cut(s)	969, 984, 999, 1014, 1394, 1424
Bsc4I	CCNNNNNNNGG	8 cut(s)	261, 455, 745, 1513, 1577, 1578, 1581, 1669
Bse1I	ACTGG	8 cut(s)	266, 292, 565, 895, 1004, 1069, 1333, 1731
Bse3DI	GCAATG	4 cut(s)	723, 798, 1366, 1630
BseBI	CCWGG	4 cut(s)	180, 548, 609, 1614
BseDI	CCNNGG	9 cut(s)	179, 607, 608, 804, 805, 1174, 1520, 1560, 1612
BseGI	GGATG	3 cut(s)	255, 296, 1444
BseLI	CCNNNNNNNGG	8 cut(s)	261, 455, 745, 1513, 1577, 1578, 1581, 1669
BseMI	GCAATG	4 cut(s)	723, 798, 1366, 1630
BseMII	CTCAG	1 cut(s)	83
BseNI	ACTGG	8 cut(s)	266, 292, 565, 895, 1004, 1069, 1333, 1731
BseRI	GAGGAG	1 cut(s)	206
BseSI	GKGCMC	1 cut(s)	1572
BseXI	GCAGC	2 cut(s)	707, 1186
BseYI	CCCAGC	3 cut(s)	662, 1335, 1656
BshFI	GGCC	7 cut(s)	184, 372, 551, 613, 880, 1266, 1662
BshNI	GGYRCC	1 cut(s)	899
BsiHKAI	GWGCWC	1 cut(s)	714
BsiHKCI	CYCGRG	2 cut(s)	186, 805
BsiSI	CCGG	2 cut(s)	806, 1575
BslFI	GGGAC	2 cut(s)	417, 822
BslI	CCNNNNNNNGG	8 cut(s)	261, 455, 745, 1513, 1577, 1578, 1581, 1669
BsmAI	GTCTC	3 cut(s)	65, 88, 776
BsmFI	GGGAC	2 cut(s)	417, 822
BsmI	GAATGC	1 cut(s)	526
BsnI	GGCC	7 cut(s)	184, 372, 551, 613, 880, 1266, 1662
Bso31I	GGTCTC	2 cut(s)	65, 776
BsoBI	CYCGRG	2 cut(s)	186, 805
Bsp1286I	GDGCHC	3 cut(s)	714, 1336, 1572
Bsp1407I	TGTACA	1 cut(s)	351
Bsp143I	GATC	5 cut(s)	208, 416, 526, 1644, 1729
BspACI	CCGC	3 cut(s)	1239, 1578, 1721
BspANI	GGCC	7 cut(s)	184, 372, 551, 613, 880, 1266, 1662
BspCNI	CTCAG	1 cut(s)	82
BspHI	TCATGA	1 cut(s)	867
BspLI	GGNNCC	8 cut(s)	433, 901, 1132, 1180, 1189, 1333, 1518, 1661
BspPI	GGATC	3 cut(s)	521, 1639, 1737
BspQI	GCTCTTC	3 cut(s)	18, 143, 719
BspT107I	GGYRCC	1 cut(s)	899
BspTNI	GGTCTC	2 cut(s)	65, 776
BsrDI	GCAATG	4 cut(s)	723, 798, 1366, 1630
BsrGI	TGTACA	1 cut(s)	351
BsrI	ACTGG	8 cut(s)	266, 292, 565, 895, 1004, 1069, 1333, 1731
BssECI	CCNNGG	9 cut(s)	179, 607, 608, 804, 805, 1174, 1520, 1560, 1612
BssMI	GATC	5 cut(s)	208, 416, 526, 1644, 1729
BssT1I	CCWWGG	2 cut(s)	1174, 1520
Bst2UI	CCWGG	4 cut(s)	180, 548, 609, 1614
Bst4CI	ACNGT	4 cut(s)	163, 170, 344, 1561
Bst6I	CTCTTC	4 cut(s)	18, 143, 719, 1455
BstAUI	TGTACA	1 cut(s)	351
BstBAI	YACGTR	1 cut(s)	886
BstC8I	GCNNGC	3 cut(s)	85, 396, 925
BstDEI	CTNAG	4 cut(s)	69, 413, 1368, 1684
BstDSI	CCRYGG	1 cut(s)	1560
BstF5I	GGATG	3 cut(s)	255, 296, 1444
BstH2I	RGCGCY	1 cut(s)	701
BstHHI	GCGC	2 cut(s)	684, 700
BstKTI	GATC	5 cut(s)	211, 419, 529, 1647, 1732
BstMAI	GTCTC	3 cut(s)	65, 88, 776
BstMBI	GATC	5 cut(s)	208, 416, 526, 1644, 1729
BstMWI	GCNNNNNNNGC	3 cut(s)	378, 1378, 1720
BstNI	CCWGG	4 cut(s)	180, 548, 609, 1614
BstNSI	RCATGY	1 cut(s)	398
BstSCI	CCNGG	6 cut(s)	178, 546, 607, 804, 805, 1612
BstSLI	GKGCMC	1 cut(s)	1572
BstV1I	GCAGC	2 cut(s)	707, 1186
BstV2I	GAAGAC	1 cut(s)	674
BstX2I	RGATCY	3 cut(s)	526, 1644, 1729
BstXI	CCANNNNNNTGG	4 cut(s)	266, 1262, 1274, 1620
BstYI	RGATCY	3 cut(s)	526, 1644, 1729
BsuRI	GGCC	7 cut(s)	184, 372, 551, 613, 880, 1266, 1662
BtgI	CCRYGG	1 cut(s)	1560
BtsCI	GGATG	3 cut(s)	255, 296, 1444
BtsIMutI	CAGTG	3 cut(s)	259, 1326, 1724
Cac8I	GCNNGC	3 cut(s)	85, 396, 925
CaiI	CAGNNNCTG	1 cut(s)	530
CciI	TCATGA	1 cut(s)	867
CfoI	GCGC	2 cut(s)	684, 700
Cfr13I	GGNCC	8 cut(s)	182, 302, 431, 569, 611, 1178, 1516, 1660
Cfr9I	CCCGGG	1 cut(s)	805
Csp6I	GTAC	2 cut(s)	352, 1365
CviAII	CATG	9 cut(s)	236, 266, 395, 408, 868, 1140, 1192, 1460, 1627
CviQI	GTAC	2 cut(s)	352, 1365
DdeI	CTNAG	4 cut(s)	69, 413, 1368, 1684
DpnI	GATC	5 cut(s)	210, 418, 528, 1646, 1731
DpnII	GATC	5 cut(s)	208, 416, 526, 1644, 1729
EaeI	YGGCCR	1 cut(s)	1264
Eam1104I	CTCTTC	4 cut(s)	18, 143, 719, 1455
EarI	CTCTTC	4 cut(s)	18, 143, 719, 1455
EciI	GGCGGA	1 cut(s)	1710
Ecl136II	GAGCTC	1 cut(s)	712
Eco130I	CCWWGG	2 cut(s)	1174, 1520
Eco147I	AGGCCT	1 cut(s)	551
Eco24I	GRGCYC	2 cut(s)	714, 1336
Eco31I	GGTCTC	2 cut(s)	65, 776
Eco32I	GATATC	1 cut(s)	112
Eco47I	GGWCC	5 cut(s)	302, 431, 569, 1178, 1516
Eco47III	AGCGCT	1 cut(s)	699
Eco53kI	GAGCTC	1 cut(s)	712
Eco57I	CTGAAG	3 cut(s)	552, 703, 1685
Eco88I	CYCGRG	2 cut(s)	186, 805
EcoICRI	GAGCTC	1 cut(s)	712
EcoO109I	RGGNCCY	3 cut(s)	1178, 1516, 1660
EcoRII	CCWGG	4 cut(s)	178, 546, 607, 1612
EcoRV	GATATC	1 cut(s)	112
EcoT14I	CCWWGG	2 cut(s)	1174, 1520
EcoT22I	ATGCAT	1 cut(s)	1632
EcoT38I	GRGCYC	2 cut(s)	714, 1336
ErhI	CCWWGG	2 cut(s)	1174, 1520
FaeI	CATG	9 cut(s)	239, 269, 398, 411, 871, 1143, 1195, 1463, 1630
FaqI	GGGAC	2 cut(s)	417, 822
FatI	CATG	9 cut(s)	235, 265, 394, 407, 867, 1139, 1191, 1459, 1626
FauI	CCCGC	1 cut(s)	1571
Fnu4HI	GCNGC	2 cut(s)	696, 1200
FokI	GGATG	3 cut(s)	242, 303, 1431
FriOI	GRGCYC	2 cut(s)	714, 1336
Fsp4HI	GCNGC	2 cut(s)	696, 1200
FspBI	CTAG	7 cut(s)	198, 896, 1395, 1422, 1521, 1601, 1653
GlaI	GCGC	2 cut(s)	683, 699
GluI	GCNGC	2 cut(s)	696, 1200
GsaI	CCCAGC	3 cut(s)	666, 1339, 1660
GsuI	CTGGAG	1 cut(s)	1350
HaeII	RGCGCY	1 cut(s)	701
HaeIII	GGCC	7 cut(s)	184, 372, 551, 613, 880, 1266, 1662
HapII	CCGG	2 cut(s)	806, 1575
HhaI	GCGC	2 cut(s)	684, 700
Hin1II	CATG	9 cut(s)	239, 269, 398, 411, 871, 1143, 1195, 1463, 1630
Hin6I	GCGC	2 cut(s)	682, 698
HinP1I	GCGC	2 cut(s)	682, 698
HincII	GTYRAC	2 cut(s)	232, 1099
HindII	GTYRAC	2 cut(s)	232, 1099
HindIII	AAGCTT	1 cut(s)	1694
HinfI	GANTC	4 cut(s)	76, 271, 603, 1153
HpaI	GTTAAC	1 cut(s)	232
HpaII	CCGG	2 cut(s)	806, 1575
HphI	GGTGA	3 cut(s)	218, 352, 1108
Hpy166II	GTNNAC	5 cut(s)	166, 232, 352, 766, 1099
Hpy188I	TCNGA	2 cut(s)	583, 1536
Hpy188III	TCNNGA	3 cut(s)	530, 781, 868
Hpy8I	GTNNAC	5 cut(s)	166, 232, 352, 766, 1099
Hpy99I	CGWCG	1 cut(s)	710
HpyAV	CCTTC	2 cut(s)	686, 787
HpyCH4III	ACNGT	4 cut(s)	163, 170, 344, 1561
HpyCH4IV	ACGT	2 cut(s)	768, 885
HpyF10VI	GCNNNNNNNGC	3 cut(s)	378, 1378, 1720
HpyF3I	CTNAG	4 cut(s)	69, 413, 1368, 1684
HpySE526I	ACGT	2 cut(s)	768, 885
Hsp92II	CATG	9 cut(s)	239, 269, 398, 411, 871, 1143, 1195, 1463, 1630
HspAI	GCGC	2 cut(s)	682, 698
KspAI	GTTAAC	1 cut(s)	232
Kzo9I	GATC	5 cut(s)	208, 416, 526, 1644, 1729
LguI	GCTCTTC	3 cut(s)	18, 143, 719
LmnI	GCTCC	1 cut(s)	1331
Lsp1109I	GCAGC	2 cut(s)	707, 1186
LweI	GCATC	2 cut(s)	574, 748
MaeI	CTAG	7 cut(s)	198, 896, 1395, 1422, 1521, 1601, 1653
MaeII	ACGT	2 cut(s)	768, 885
MaeIII	GTNAC	5 cut(s)	49, 127, 140, 157, 886
MalI	GATC	5 cut(s)	210, 418, 528, 1646, 1731
MboI	GATC	5 cut(s)	208, 416, 526, 1644, 1729
MflI	RGATCY	3 cut(s)	526, 1644, 1729
MhlI	GDGCHC	3 cut(s)	714, 1336, 1572
MlsI	TGGCCA	1 cut(s)	1266
MluCI	AATT	7 cut(s)	173, 996, 1034, 1291, 1311, 1321, 1468
MluNI	TGGCCA	1 cut(s)	1266
MlyI	GAGTC	2 cut(s)	70, 597
Mox20I	TGGCCA	1 cut(s)	1266
Mph1103I	ATGCAT	1 cut(s)	1632
MroXI	GAANNNNTTC	1 cut(s)	1696
MscI	TGGCCA	1 cut(s)	1266
MseI	TTAA	6 cut(s)	27, 231, 1310, 1320, 1389, 1467
MslI	CAYNNNNRTG	4 cut(s)	224, 264, 1397, 1458
Msp20I	TGGCCA	1 cut(s)	1266
MspA1I	CMGCKG	1 cut(s)	1339
MspI	CCGG	2 cut(s)	806, 1575
MspR9I	CCNGG	6 cut(s)	180, 548, 609, 806, 807, 1614
Mva1269I	GAATGC	1 cut(s)	526
MvaI	CCWGG	4 cut(s)	180, 548, 609, 1614
MwoI	GCNNNNNNNGC	3 cut(s)	378, 1378, 1720
NciI	CCSGG	2 cut(s)	806, 807
NdeII	GATC	5 cut(s)	208, 416, 526, 1644, 1729
NlaIII	CATG	9 cut(s)	239, 269, 398, 411, 871, 1143, 1195, 1463, 1630
NlaIV	GGNNCC	8 cut(s)	433, 901, 1132, 1180, 1189, 1333, 1518, 1661
NmuCI	GTSAC	3 cut(s)	127, 140, 886
NsiI	ATGCAT	1 cut(s)	1632
NspI	RCATGY	1 cut(s)	398
OliI	CACNNNNGTG	1 cut(s)	1397
PaeI	GCATGC	1 cut(s)	398
PaeR7I	CTCGAG	1 cut(s)	186
PagI	TCATGA	1 cut(s)	867
PasI	CCCWGGG	1 cut(s)	608
PceI	AGGCCT	1 cut(s)	551
PciSI	GCTCTTC	3 cut(s)	18, 143, 719
PctI	GAATGC	1 cut(s)	526
PdmI	GAANNNNTTC	1 cut(s)	1696
PfeI	GAWTC	2 cut(s)	271, 1153
PflMI	CCANNNNNTGG	1 cut(s)	261
PkrI	GCNGC	2 cut(s)	697, 1201
PleI	GAGTC	2 cut(s)	70, 597
PpsI	GAGTC	2 cut(s)	70, 597
Ppu21I	YACGTR	1 cut(s)	886
PpuMI	RGGWCCY	2 cut(s)	1178, 1516
Psp124BI	GAGCTC	1 cut(s)	714
Psp5II	RGGWCCY	2 cut(s)	1178, 1516
Psp6I	CCWGG	4 cut(s)	178, 546, 607, 1612
PspFI	CCCAGC	3 cut(s)	662, 1335, 1656
PspGI	CCWGG	4 cut(s)	178, 546, 607, 1612
PspN4I	GGNNCC	8 cut(s)	433, 901, 1132, 1180, 1189, 1333, 1518, 1661
PspPI	GGNCC	8 cut(s)	182, 302, 431, 569, 611, 1178, 1516, 1660
PspPPI	RGGWCCY	2 cut(s)	1178, 1516
PspXI	VCTCGAGB	1 cut(s)	186
PstNI	CAGNNNCTG	1 cut(s)	530
PsuI	RGATCY	3 cut(s)	526, 1644, 1729
PvuII	CAGCTG	1 cut(s)	1339
RsaI	GTAC	2 cut(s)	353, 1366
RsaNI	GTAC	2 cut(s)	352, 1365
RseI	CAYNNNNRTG	4 cut(s)	224, 264, 1397, 1458
SacI	GAGCTC	1 cut(s)	714
SapI	GCTCTTC	3 cut(s)	18, 143, 719
SaqAI	TTAA	6 cut(s)	27, 231, 1310, 1320, 1389, 1467
SatI	GCNGC	2 cut(s)	696, 1200
Sau3AI	GATC	5 cut(s)	208, 416, 526, 1644, 1729
Sau96I	GGNCC	8 cut(s)	182, 302, 431, 569, 611, 1178, 1516, 1660
SchI	GAGTC	2 cut(s)	70, 597
ScrFI	CCNGG	6 cut(s)	180, 548, 609, 806, 807, 1614
SduI	GDGCHC	3 cut(s)	714, 1336, 1572
SfaNI	GCATC	2 cut(s)	574, 748
Sfr274I	CTCGAG	1 cut(s)	186
SinI	GGWCC	5 cut(s)	302, 431, 569, 1178, 1516
SlaI	CTCGAG	1 cut(s)	186
SmaI	CCCGGG	1 cut(s)	807
SmiMI	CAYNNNNRTG	4 cut(s)	224, 264, 1397, 1458
SmlI	CTYRAG	2 cut(s)	186, 1640
SmoI	CTYRAG	2 cut(s)	186, 1640
SpeI	ACTAGT	2 cut(s)	1394, 1421
SphI	GCATGC	1 cut(s)	398
Sse9I	AATT	7 cut(s)	173, 996, 1034, 1291, 1311, 1321, 1468
SseBI	AGGCCT	1 cut(s)	551
SsiI	CCGC	3 cut(s)	1239, 1578, 1721
SspI	AATATT	1 cut(s)	1216
SspMI	CTAG	7 cut(s)	198, 896, 1395, 1422, 1521, 1601, 1653
SstI	GAGCTC	1 cut(s)	714
StuI	AGGCCT	1 cut(s)	551
StyD4I	CCNGG	6 cut(s)	178, 546, 607, 804, 805, 1612
StyI	CCWWGG	2 cut(s)	1174, 1520
TaaI	ACNGT	4 cut(s)	163, 170, 344, 1561
TaiI	ACGT	2 cut(s)	771, 888
TaqI	TCGA	4 cut(s)	187, 274, 780, 1503
TasI	AATT	7 cut(s)	173, 996, 1034, 1291, 1311, 1321, 1468
TatI	WGTACW	1 cut(s)	351
TfiI	GAWTC	2 cut(s)	271, 1153
Tru1I	TTAA	6 cut(s)	27, 231, 1310, 1320, 1389, 1467
Tru9I	TTAA	6 cut(s)	27, 231, 1310, 1320, 1389, 1467
TscAI	CASTG	3 cut(s)	266, 1333, 1731
TseFI	GTSAC	3 cut(s)	127, 140, 886
TseI	GCWGC	2 cut(s)	695, 1199
Tsp45I	GTSAC	3 cut(s)	127, 140, 886
TspDTI	ATGAA	8 cut(s)	134, 396, 856, 944, 986, 1442, 1448, 1615
TspGWI	ACGGA	1 cut(s)	1040
TspMI	CCCGGG	1 cut(s)	805
TspRI	CASTG	3 cut(s)	266, 1333, 1731
Van91I	CCANNNNNTGG	1 cut(s)	261
VpaK11BI	GGWCC	5 cut(s)	302, 431, 569, 1178, 1516
XapI	RAATTY	1 cut(s)	1034
XceI	RCATGY	1 cut(s)	398
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1181
XhoI	CTCGAG	1 cut(s)	186
XmaI	CCCGGG	1 cut(s)	805
XmaJI	CCTAGG	1 cut(s)	1520
XmnI	GAANNNNTTC	1 cut(s)	1696
XspI	CTAG	7 cut(s)	198, 896, 1395, 1422, 1521, 1601, 1653
Zsp2I	ATGCAT	1 cut(s)	1632

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.