RLG00000035518

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000610
Physical Location & Seq
Reverse (-)
67932062 .. 67933428
1367 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035518

Sequence Viewer

Length: 774 bp
ATGCCACATTTCTCATGCATGACCGCAAGGCCATACGTGACTGGTCTAGGCACCTTTGACAACTCCACTGTTTCTGCCATTCTAGAGTACGAAACGACTACACTTCACTCAATCCTCCCATTGAACAAACTTCCGCTCCACAAACCAATTCTCCCAGCTCTAAACGTCGACACATCGTTTGCAACAAAGTTCGCCAGCAAACTCCGCAGCTTAAACACTCTAGAGTTCCCAGCTAACGTCCCGCAGAAAGTTGACAAGCGTTTGTTCTTCACAATAGGACTTGGAACCAACCCATGTAGCCAGCAGCAGCACAACAACCAAACCTGCCAAGGACCCAATGGAACCATGTTTGCTGCTTCAATCAACAACGTTTCTTTTACTATGCCAAACACTGCCTTACTCCAATCCCACTTTTCTGGCCAAGCAAAGGGAGTTTATTCCCCTGACTTTCCGATCAGGCCTTCAATTCCATTTAATTACACAGGGATCCATCTGAACAATACTAATGTGAGCAATGGGTTTGGCAACTTTGACCAGAATTATGACCCTAGGAAGTTCAATCTGGTTGACCCCGTTGAAAGAAACACGGTGGGTGTGCCCTCTGGTGGGTGGGTTGCCATTCGGTTTCTAGCAGATAACCCAGGTGTCTGGTTCATGCATTGCCACTTGGAAATCCACACTAGCTGGGGCTTGAAGATGGCTTGGGTAGTCTTAGATGGAGAGCTTCCCAATCAAAAGTTGCTTCCTCCACCACTGGATCTTCCTAAATGCTGA

Protein Analysis

258

Amino Acids

28.45

Weight (kDa)

8.28

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_2 PF07731 163 - 239 3e-24 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000610)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60020 AT5G60020 AT5G60020
fragaria_vesca FvH4_2g09062 FvH4_2g09062 FvH4_3g05920 FvH4_3g34100 FvH4_3g34310 FvH4_3g34340 FvH4_6g13390
malus_domestica MD00G1015000.v1.1 MD03G1106500.v1.1 MD03G1106600.v1.1 MD04G1131500.v1.1 MD11G1118900.v1.1 MD11G1119000.v1.1 MD12G1144600.v1.1
prunus_persica Prupe.6G089100_v2.0.a1 Prupe.6G089300_v2.0.a1 Prupe.6G258000_v2.0.a1
pyrus_communis pycom03g08590 pycom10g02940 pycom11g09990 pycom11g10060
rosa_chinensis RchiOBHm_Chr2g0154491 RchiOBHm_Chr3g0466271 RchiOBHm_Chr5g0061891 RchiOBHm_Chr5g0061911 RchiOBHm_Chr5g0061951 RchiOBHm_Chr5g0061961 RchiOBHm_Chr6g0264371
rosa_laevigata RLG00000024591 RLG00000035514 RLG00000035516 RLG00000035518 RLG00000035519
rosa_multiflora Rmu_sc0003480.1_g000021 Rmu_sc0011641.1_g000002 Rmu_sc0011641.1_g000009
rosa_roxburghii Rroxscaffold_1G00018790 Rroxscaffold_1G00018820 Rroxscaffold_1G00018850 Rroxscaffold_2G00122520 Rroxscaffold_2G00122540 Rroxscaffold_6G00414660
rosa_rugosa Rorug03G0080700 Rorug04G0180900 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345100 Rorug05G0345200 Rorug06G0011800
rosa_samantha Rh3DG148900 Rh5AG405700 Rh5AG406100 Rh5AG406200 Rh5BG419200 Rh5BG419400 Rh5CG443100 Rh5CG443300 Rh5CG443500 Rh5CG443600 Rh5DG432700 Rh5DG434900 Rh6BG128700 Rh6CG127600 Rh6DG114200 Rh6DG114300
rosa_wichuraiana Rw3G011980 Rw5G038100 Rw5G038140 Rw5G038160 Rw5G038200 Rw5G038210 Rw5G038250 Rw5G038270 Rw5G038290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 332
AccB1I GGYRCC 1 cut(s) 50
AccBSI CCGCTC 1 cut(s) 136
AccI GTMKAC 1 cut(s) 168
AciI CCGC 4 cut(s) 24, 134, 205, 242
AclI AACGTT 1 cut(s) 369
AclWI GGATC 3 cut(s) 481, 494, 765
AcoI YGGCCR 1 cut(s) 418
AfaI GTAC 1 cut(s) 89
AfiI CCNNNNNNNGG 3 cut(s) 427, 605, 606
AgsI TTSAA 6 cut(s) 124, 360, 465, 559, 578, 694
AjnI CCWGG 1 cut(s) 640
AluBI AGCT 5 cut(s) 158, 210, 233, 684, 724
AluI AGCT 5 cut(s) 158, 210, 233, 684, 724
AlwI GGATC 3 cut(s) 481, 494, 765
AoxI GGCC 3 cut(s) 29, 418, 458
ApeKI GCWGC 4 cut(s) 207, 304, 307, 353
ArsI GACNNNNNNTTYG 2 cut(s) 161, 193
AspA2I CCTAGG 1 cut(s) 548
AspS9I GGNCC 1 cut(s) 332
AvaII GGWCC 1 cut(s) 332
AvrII CCTAGG 1 cut(s) 548
BaeGI GKGCMC 1 cut(s) 600
BalI TGGCCA 1 cut(s) 420
BamHI GGATCC 1 cut(s) 486
BanI GGYRCC 1 cut(s) 50
BbvI GCAGC 4 cut(s) 219, 316, 319, 340
BccI CCATC 3 cut(s) 498, 691, 710
BciT130I CCWGG 1 cut(s) 642
BfaI CTAG 6 cut(s) 47, 83, 221, 549, 629, 681
BfuAI ACCTGC 1 cut(s) 332
BisI GCNGC 4 cut(s) 208, 305, 308, 354
BlnI CCTAGG 1 cut(s) 548
BlsI GCNGC 4 cut(s) 209, 306, 309, 355
Bme1390I CCNGG 1 cut(s) 642
Bme18I GGWCC 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 5 cut(s) 52, 286, 334, 343, 488
BmrFI CCNGG 1 cut(s) 642
BsaAI YACGTR 1 cut(s) 37
BsaJI CCNNGG 3 cut(s) 328, 548, 640
BsaXI ACNNNNNCTCC 4 cut(s) 120, 135, 150, 165
Bsc4I CCNNNNNNNGG 3 cut(s) 427, 605, 606
Bse1I ACTGG 2 cut(s) 46, 759
Bse3DI GCAATG 2 cut(s) 520, 658
BseBI CCWGG 1 cut(s) 642
BseDI CCNNGG 3 cut(s) 328, 548, 640
BseLI CCNNNNNNNGG 3 cut(s) 427, 605, 606
BseMI GCAATG 2 cut(s) 520, 658
BseNI ACTGG 2 cut(s) 46, 759
BseSI GKGCMC 1 cut(s) 600
BseXI GCAGC 4 cut(s) 219, 316, 319, 340
BseYI CCCAGC 3 cut(s) 154, 229, 684
BshFI GGCC 3 cut(s) 31, 420, 460
BshNI GGYRCC 1 cut(s) 50
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 3 cut(s) 427, 605, 606
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 3 cut(s) 31, 420, 460
Bsp1286I GDGCHC 1 cut(s) 600
Bsp143I GATC 3 cut(s) 453, 486, 757
BspACI CCGC 4 cut(s) 24, 134, 205, 242
BspANI GGCC 3 cut(s) 31, 420, 460
BspLI GGNNCC 5 cut(s) 52, 286, 334, 343, 488
BspMI ACCTGC 1 cut(s) 332
BspPI GGATC 3 cut(s) 481, 494, 765
BspT107I GGYRCC 1 cut(s) 50
BsrBI CCGCTC 1 cut(s) 136
BsrDI GCAATG 2 cut(s) 520, 658
BsrI ACTGG 2 cut(s) 46, 759
BssECI CCNNGG 3 cut(s) 328, 548, 640
BssMI GATC 3 cut(s) 453, 486, 757
BssT1I CCWWGG 2 cut(s) 328, 548
Bst2UI CCWGG 1 cut(s) 642
Bst4CI ACNGT 2 cut(s) 70, 589
BstBAI YACGTR 1 cut(s) 37
BstC8I GCNNGC 2 cut(s) 196, 302
BstDEI CTNAG 1 cut(s) 712
BstKTI GATC 3 cut(s) 456, 489, 760
BstMBI GATC 3 cut(s) 453, 486, 757
BstMWI GCNNNNNNNGC 1 cut(s) 204
BstNI CCWGG 1 cut(s) 642
BstSCI CCNGG 1 cut(s) 640
BstSLI GKGCMC 1 cut(s) 600
BstV1I GCAGC 4 cut(s) 219, 316, 319, 340
BstX2I RGATCY 2 cut(s) 486, 757
BstXI CCANNNNNNTGG 2 cut(s) 416, 648
BstYI RGATCY 2 cut(s) 486, 757
BsuRI GGCC 3 cut(s) 31, 420, 460
BtsI GCAGTG 1 cut(s) 390
BtsIMutI CAGTG 3 cut(s) 66, 390, 752
BveI ACCTGC 1 cut(s) 332
Cac8I GCNNGC 2 cut(s) 196, 302
Cfr13I GGNCC 1 cut(s) 332
Csp6I GTAC 1 cut(s) 88
CviAII CATG 5 cut(s) 15, 19, 294, 346, 655
CviQI GTAC 1 cut(s) 88
DdeI CTNAG 1 cut(s) 712
DpnI GATC 3 cut(s) 455, 488, 759
DpnII GATC 3 cut(s) 453, 486, 757
EaeI YGGCCR 1 cut(s) 418
Eco130I CCWWGG 2 cut(s) 328, 548
Eco147I AGGCCT 1 cut(s) 460
Eco47I GGWCC 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 640
EcoT14I CCWWGG 2 cut(s) 328, 548
EcoT22I ATGCAT 2 cut(s) 20, 660
ErhI CCWWGG 2 cut(s) 328, 548
FaeI CATG 5 cut(s) 18, 22, 297, 349, 658
FaiI YATR 8 cut(s) 16, 20, 34, 295, 347, 383, 543, 656
FaqI GGGAC 1 cut(s) 224
FatI CATG 5 cut(s) 14, 18, 293, 345, 654
FauI CCCGC 1 cut(s) 249
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 4 cut(s) 208, 305, 308, 354
Fsp4HI GCNGC 4 cut(s) 208, 305, 308, 354
FspBI CTAG 6 cut(s) 47, 83, 221, 549, 629, 681
GluI GCNGC 4 cut(s) 208, 305, 308, 354
GsaI CCCAGC 3 cut(s) 158, 233, 688
HaeIII GGCC 3 cut(s) 31, 420, 460
Hin1II CATG 5 cut(s) 18, 22, 297, 349, 658
HincII GTYRAC 3 cut(s) 169, 253, 568
HindII GTYRAC 3 cut(s) 169, 253, 568
Hpy166II GTNNAC 3 cut(s) 169, 253, 568
Hpy188I TCNGA 2 cut(s) 453, 495
Hpy188III TCNNGA 2 cut(s) 83, 221
Hpy8I GTNNAC 3 cut(s) 169, 253, 568
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 1 cut(s) 471
HpyCH4III ACNGT 2 cut(s) 70, 589
HpyCH4IV ACGT 4 cut(s) 36, 165, 237, 369
HpyCH4V TGCA 3 cut(s) 18, 182, 658
HpyF10VI GCNNNNNNNGC 1 cut(s) 204
HpyF3I CTNAG 1 cut(s) 712
HpySE526I ACGT 4 cut(s) 36, 165, 237, 369
Hsp92II CATG 5 cut(s) 18, 22, 297, 349, 658
Kzo9I GATC 3 cut(s) 453, 486, 757
LmnI GCTCC 1 cut(s) 141
Lsp1109I GCAGC 4 cut(s) 219, 316, 319, 340
MaeI CTAG 6 cut(s) 47, 83, 221, 549, 629, 681
MaeII ACGT 4 cut(s) 36, 165, 237, 369
MaeIII GTNAC 1 cut(s) 37
MalI GATC 3 cut(s) 455, 488, 759
MbiI CCGCTC 1 cut(s) 136
MboI GATC 3 cut(s) 453, 486, 757
MboII GAAGA 3 cut(s) 259, 706, 752
MflI RGATCY 2 cut(s) 486, 757
MhlI GDGCHC 1 cut(s) 600
MlsI TGGCCA 1 cut(s) 420
MluCI AATT 4 cut(s) 147, 465, 475, 538
MluNI TGGCCA 1 cut(s) 420
MnlI CCTC 3 cut(s) 125, 610, 756
Mox20I TGGCCA 1 cut(s) 420
Mph1103I ATGCAT 2 cut(s) 20, 660
MscI TGGCCA 1 cut(s) 420
MseI TTAA 2 cut(s) 212, 474
Msp20I TGGCCA 1 cut(s) 420
MspR9I CCNGG 1 cut(s) 642
MvaI CCWGG 1 cut(s) 642
MwoI GCNNNNNNNGC 1 cut(s) 204
NdeII GATC 3 cut(s) 453, 486, 757
NlaIII CATG 5 cut(s) 18, 22, 297, 349, 658
NlaIV GGNNCC 5 cut(s) 52, 286, 334, 343, 488
NmuCI GTSAC 1 cut(s) 37
NsiI ATGCAT 2 cut(s) 20, 660
PceI AGGCCT 1 cut(s) 460
PkrI GCNGC 4 cut(s) 209, 306, 309, 355
Ppu21I YACGTR 1 cut(s) 37
PpuMI RGGWCCY 1 cut(s) 332
Psp1406I AACGTT 1 cut(s) 369
Psp5II RGGWCCY 1 cut(s) 332
Psp6I CCWGG 1 cut(s) 640
PspFI CCCAGC 3 cut(s) 154, 229, 684
PspGI CCWGG 1 cut(s) 640
PspN4I GGNNCC 5 cut(s) 52, 286, 334, 343, 488
PspPI GGNCC 1 cut(s) 332
PspPPI RGGWCCY 1 cut(s) 332
PsuI RGATCY 2 cut(s) 486, 757
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
SalI GTCGAC 1 cut(s) 167
SaqAI TTAA 2 cut(s) 212, 474
SatI GCNGC 4 cut(s) 208, 305, 308, 354
Sau3AI GATC 3 cut(s) 453, 486, 757
Sau96I GGNCC 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 642
SduI GDGCHC 1 cut(s) 600
SinI GGWCC 1 cut(s) 332
Sse9I AATT 4 cut(s) 147, 465, 475, 538
SseBI AGGCCT 1 cut(s) 460
SsiI CCGC 4 cut(s) 24, 134, 205, 242
SspMI CTAG 6 cut(s) 47, 83, 221, 549, 629, 681
StuI AGGCCT 1 cut(s) 460
StyD4I CCNGG 1 cut(s) 640
StyI CCWWGG 2 cut(s) 328, 548
TaaI ACNGT 2 cut(s) 70, 589
TaiI ACGT 4 cut(s) 39, 168, 240, 372
TaqI TCGA 1 cut(s) 168
TasI AATT 4 cut(s) 147, 465, 475, 538
Tru1I TTAA 2 cut(s) 212, 474
Tru9I TTAA 2 cut(s) 212, 474
TscAI CASTG 3 cut(s) 73, 397, 759
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 4 cut(s) 207, 304, 307, 353
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 643
TspRI CASTG 3 cut(s) 73, 397, 759
VpaK11BI GGWCC 1 cut(s) 332
XbaI TCTAGA 2 cut(s) 82, 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 335
XmaJI CCTAGG 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 168
XspI CTAG 6 cut(s) 47, 83, 221, 549, 629, 681
Zsp2I ATGCAT 2 cut(s) 20, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.