Lignin degradation and detoxification of lignin-derived products
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 389, 908 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 270, 464 |
| AciI | CCGC | 4 cut(s) | 882, 957, 1060, 1587 |
| AclI | AACGTT | 2 cut(s) | 831, 1224 |
| AclWI | GGATC | 3 cut(s) | 288, 530, 1648 |
| AcoI | YGGCCR | 1 cut(s) | 1273 |
| AcuI | CTGAAG | 3 cut(s) | 561, 680, 712 |
| AcyI | GRCGYC | 2 cut(s) | 594, 768 |
| AfaI | GTAC | 5 cut(s) | 362, 504, 629, 947, 1375 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 195, 270, 399, 464, 754, 1282, 1586, 1587, 1590 |
| AflIII | ACRYGT | 2 cut(s) | 111, 1748 |
| AgsI | TTSAA | 9 cut(s) | 119, 655, 668, 1215, 1330, 1477, 1540, 1559, 1675 |
| AhlI | ACTAGT | 2 cut(s) | 1403, 1430 |
| AjnI | CCWGG | 4 cut(s) | 101, 555, 616, 1621 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 635, 667, 839, 871 |
| Alw21I | GWGCWC | 1 cut(s) | 723 |
| Alw26I | GTCTC | 3 cut(s) | 13, 74, 592 |
| AlwI | GGATC | 3 cut(s) | 288, 530, 1648 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 539 |
| Ama87I | CYCGRG | 1 cut(s) | 814 |
| AoxI | GGCC | 5 cut(s) | 303, 311, 577, 887, 1273 |
| ApeKI | GCWGC | 5 cut(s) | 704, 1062, 1159, 1162, 1208 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1016, 1048 |
| AseI | ATTAAT | 1 cut(s) | 606 |
| AspS9I | GGNCC | 7 cut(s) | 191, 303, 311, 440, 578, 620, 1187 |
| AsuC2I | CCSGG | 4 cut(s) | 189, 298, 815, 816 |
| AvaI | CYCGRG | 1 cut(s) | 814 |
| AvaII | GGWCC | 4 cut(s) | 191, 440, 620, 1187 |
| BalI | TGGCCA | 1 cut(s) | 1275 |
| BanI | GGYRCC | 2 cut(s) | 389, 908 |
| BanII | GRGCYC | 2 cut(s) | 723, 1345 |
| Bbv12I | GWGCWC | 1 cut(s) | 723 |
| BbvI | GCAGC | 5 cut(s) | 716, 1074, 1171, 1174, 1195 |
| BccI | CCATC | 5 cut(s) | 272, 302, 515, 1672, 1691 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 263, 297 |
| BciT130I | CCWGG | 4 cut(s) | 103, 557, 618, 1623 |
| BcnI | CCSGG | 4 cut(s) | 189, 298, 815, 816 |
| BcoDI | GTCTC | 3 cut(s) | 13, 74, 592 |
| BcuI | ACTAGT | 2 cut(s) | 1403, 1430 |
| BfaI | CTAG | 7 cut(s) | 905, 941, 1404, 1431, 1610, 1662, 1694 |
| BglII | AGATCT | 1 cut(s) | 1738 |
| BisI | GCNGC | 5 cut(s) | 705, 1063, 1160, 1163, 1209 |
| BlpI | GCTNAGC | 1 cut(s) | 1344 |
| BlsI | GCNGC | 5 cut(s) | 706, 1064, 1161, 1164, 1210 |
| Bme1390I | CCNGG | 8 cut(s) | 103, 189, 298, 557, 618, 815, 816, 1623 |
| Bme18I | GGWCC | 4 cut(s) | 191, 440, 620, 1187 |
| BmeT110I | CYCGRG | 1 cut(s) | 814 |
| BmgT120I | GGNCC | 7 cut(s) | 191, 303, 311, 440, 578, 620, 1187 |
| BmiI | GGNNCC | 5 cut(s) | 391, 442, 910, 1141, 1189 |
| BmrFI | CCNGG | 8 cut(s) | 103, 189, 298, 557, 618, 815, 816, 1623 |
| BmrI | ACTGGG | 1 cut(s) | 1347 |
| BmuI | ACTGGG | 1 cut(s) | 1347 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1344 |
| BpuMI | CCSGG | 4 cut(s) | 189, 298, 815, 816 |
| BsaAI | YACGTR | 1 cut(s) | 895 |
| BsaHI | GRCGYC | 2 cut(s) | 594, 768 |
| BsaJI | CCNNGG | 5 cut(s) | 194, 813, 814, 1183, 1621 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 245, 275, 978, 993, 1008, 1023, 1403, 1433 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 195, 270, 399, 464, 754, 1282, 1586, 1587, 1590 |
| Bse118I | RCCGGY | 1 cut(s) | 313 |
| Bse1I | ACTGG | 3 cut(s) | 574, 904, 1342 |
| Bse3DI | GCAATG | 3 cut(s) | 20, 732, 807 |
| BseBI | CCWGG | 4 cut(s) | 103, 557, 618, 1623 |
| BseDI | CCNNGG | 5 cut(s) | 194, 813, 814, 1183, 1621 |
| BseGI | GGATG | 2 cut(s) | 264, 1453 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 195, 270, 399, 464, 754, 1282, 1586, 1587, 1590 |
| BseMI | GCAATG | 3 cut(s) | 20, 732, 807 |
| BseMII | CTCAG | 3 cut(s) | 69, 436, 1358 |
| BseNI | ACTGG | 3 cut(s) | 574, 904, 1342 |
| BseXI | GCAGC | 5 cut(s) | 716, 1074, 1171, 1174, 1195 |
| BseYI | CCCAGC | 4 cut(s) | 671, 1012, 1084, 1665 |
| Bsh1285I | CGRYCG | 1 cut(s) | 957 |
| BshFI | GGCC | 5 cut(s) | 305, 313, 579, 889, 1275 |
| BshNI | GGYRCC | 2 cut(s) | 389, 908 |
| BsiEI | CGRYCG | 1 cut(s) | 957 |
| BsiHKAI | GWGCWC | 1 cut(s) | 723 |
| BsiHKCI | CYCGRG | 1 cut(s) | 814 |
| BsiSI | CCGG | 5 cut(s) | 189, 297, 314, 815, 1584 |
| BslFI | GGGAC | 3 cut(s) | 426, 831, 1079 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 195, 270, 399, 464, 754, 1282, 1586, 1587, 1590 |
| BsmAI | GTCTC | 3 cut(s) | 13, 74, 592 |
| BsmFI | GGGAC | 3 cut(s) | 426, 831, 1079 |
| BsmI | GAATGC | 2 cut(s) | 535, 936 |
| BsnI | GGCC | 5 cut(s) | 305, 313, 579, 889, 1275 |
| BsoBI | CYCGRG | 1 cut(s) | 814 |
| Bsp1286I | GDGCHC | 2 cut(s) | 723, 1345 |
| Bsp1407I | TGTACA | 2 cut(s) | 360, 627 |
| Bsp143I | GATC | 5 cut(s) | 293, 425, 535, 1653, 1738 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1344 |
| BspACI | CCGC | 4 cut(s) | 882, 957, 1060, 1587 |
| BspANI | GGCC | 5 cut(s) | 305, 313, 579, 889, 1275 |
| BspCNI | CTCAG | 3 cut(s) | 70, 435, 1357 |
| BspHI | TCATGA | 1 cut(s) | 876 |
| BspLI | GGNNCC | 5 cut(s) | 391, 442, 910, 1141, 1189 |
| BspPI | GGATC | 3 cut(s) | 288, 530, 1648 |
| BspQI | GCTCTTC | 1 cut(s) | 728 |
| BspT107I | GGYRCC | 2 cut(s) | 389, 908 |
| BsrDI | GCAATG | 3 cut(s) | 20, 732, 807 |
| BsrFI | RCCGGY | 1 cut(s) | 313 |
| BsrGI | TGTACA | 2 cut(s) | 360, 627 |
| BsrI | ACTGG | 3 cut(s) | 574, 904, 1342 |
| BssAI | RCCGGY | 1 cut(s) | 313 |
| BssECI | CCNNGG | 5 cut(s) | 194, 813, 814, 1183, 1621 |
| BssMI | GATC | 5 cut(s) | 293, 425, 535, 1653, 1738 |
| BssNI | GRCGYC | 2 cut(s) | 594, 768 |
| BssT1I | CCWWGG | 1 cut(s) | 1183 |
| Bst2UI | CCWGG | 4 cut(s) | 103, 557, 618, 1623 |
| Bst4CI | ACNGT | 8 cut(s) | 172, 353, 433, 635, 1080, 1373, 1570, 1734 |
| Bst6I | CTCTTC | 3 cut(s) | 53, 728, 1464 |
| BstACI | GRCGYC | 2 cut(s) | 594, 768 |
| BstAUI | TGTACA | 2 cut(s) | 360, 627 |
| BstBAI | YACGTR | 1 cut(s) | 895 |
| BstC8I | GCNNGC | 8 cut(s) | 27, 84, 94, 315, 405, 934, 1051, 1157 |
| BstDEI | CTNAG | 4 cut(s) | 78, 422, 1344, 1530 |
| BstF5I | GGATG | 2 cut(s) | 264, 1453 |
| BstKTI | GATC | 5 cut(s) | 296, 428, 538, 1656, 1741 |
| BstMAI | GTCTC | 3 cut(s) | 13, 74, 592 |
| BstMBI | GATC | 5 cut(s) | 293, 425, 535, 1653, 1738 |
| BstMCI | CGRYCG | 1 cut(s) | 957 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1059, 1152, 1387 |
| BstNI | CCWGG | 4 cut(s) | 103, 557, 618, 1623 |
| BstNSI | RCATGY | 3 cut(s) | 115, 407, 1752 |
| BstSCI | CCNGG | 8 cut(s) | 101, 187, 296, 555, 616, 813, 814, 1621 |
| BstV1I | GCAGC | 5 cut(s) | 716, 1074, 1171, 1174, 1195 |
| BstX2I | RGATCY | 3 cut(s) | 535, 1653, 1738 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 275, 1271 |
| BstYI | RGATCY | 3 cut(s) | 535, 1653, 1738 |
| BsuRI | GGCC | 5 cut(s) | 305, 313, 579, 889, 1275 |
| BtsCI | GGATG | 2 cut(s) | 264, 1453 |
| BtsI | GCAGTG | 1 cut(s) | 1245 |
| BtsIMutI | CAGTG | 3 cut(s) | 977, 1245, 1335 |
| Cac8I | GCNNGC | 8 cut(s) | 27, 84, 94, 315, 405, 934, 1051, 1157 |
| CaiI | CAGNNNCTG | 1 cut(s) | 539 |
| CciI | TCATGA | 1 cut(s) | 876 |
| Cfr10I | RCCGGY | 1 cut(s) | 313 |
| Cfr13I | GGNCC | 7 cut(s) | 191, 303, 311, 440, 578, 620, 1187 |
| Cfr9I | CCCGGG | 1 cut(s) | 814 |
| CseI | GACGC | 2 cut(s) | 602, 776 |
| Csp6I | GTAC | 5 cut(s) | 361, 503, 628, 946, 1374 |
| CviQI | GTAC | 5 cut(s) | 361, 503, 628, 946, 1374 |
| DdeI | CTNAG | 4 cut(s) | 78, 422, 1344, 1530 |
| DpnI | GATC | 5 cut(s) | 295, 427, 537, 1655, 1740 |
| DpnII | GATC | 5 cut(s) | 293, 425, 535, 1653, 1738 |
| EaeI | YGGCCR | 1 cut(s) | 1273 |
| Eam1104I | CTCTTC | 3 cut(s) | 53, 728, 1464 |
| EarI | CTCTTC | 3 cut(s) | 53, 728, 1464 |
| Ecl136II | GAGCTC | 1 cut(s) | 721 |
| Eco130I | CCWWGG | 1 cut(s) | 1183 |
| Eco24I | GRGCYC | 2 cut(s) | 723, 1345 |
| Eco47I | GGWCC | 4 cut(s) | 191, 440, 620, 1187 |
| Eco53kI | GAGCTC | 1 cut(s) | 721 |
| Eco57I | CTGAAG | 3 cut(s) | 561, 680, 712 |
| Eco88I | CYCGRG | 1 cut(s) | 814 |
| EcoICRI | GAGCTC | 1 cut(s) | 721 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1187 |
| EcoRII | CCWGG | 4 cut(s) | 101, 555, 616, 1621 |
| EcoT14I | CCWWGG | 1 cut(s) | 1183 |
| EcoT22I | ATGCAT | 1 cut(s) | 1641 |
| EcoT38I | GRGCYC | 2 cut(s) | 723, 1345 |
| ErhI | CCWWGG | 1 cut(s) | 1183 |
| FaqI | GGGAC | 3 cut(s) | 426, 831, 1079 |
| FauI | CCCGC | 1 cut(s) | 1580 |
| FauNDI | CATATG | 1 cut(s) | 319 |
| Fnu4HI | GCNGC | 5 cut(s) | 705, 1063, 1160, 1163, 1209 |
| FokI | GGATG | 2 cut(s) | 251, 1440 |
| FriOI | GRGCYC | 2 cut(s) | 723, 1345 |
| Fsp4HI | GCNGC | 5 cut(s) | 705, 1063, 1160, 1163, 1209 |
| FspBI | CTAG | 7 cut(s) | 905, 941, 1404, 1431, 1610, 1662, 1694 |
| GluI | GCNGC | 5 cut(s) | 705, 1063, 1160, 1163, 1209 |
| GsaI | CCCAGC | 4 cut(s) | 675, 1016, 1088, 1669 |
| HaeIII | GGCC | 5 cut(s) | 305, 313, 579, 889, 1275 |
| HapII | CCGG | 5 cut(s) | 189, 297, 314, 815, 1584 |
| HgaI | GACGC | 2 cut(s) | 602, 776 |
| Hin1I | GRCGYC | 2 cut(s) | 594, 768 |
| HincII | GTYRAC | 2 cut(s) | 1108, 1549 |
| HindII | GTYRAC | 2 cut(s) | 1108, 1549 |
| HinfI | GANTC | 2 cut(s) | 280, 1473 |
| HpaII | CCGG | 5 cut(s) | 189, 297, 314, 815, 1584 |
| Hpy166II | GTNNAC | 4 cut(s) | 250, 361, 1108, 1549 |
| Hpy188I | TCNGA | 5 cut(s) | 79, 198, 425, 592, 1738 |
| Hpy188III | TCNNGA | 3 cut(s) | 539, 877, 941 |
| Hpy8I | GTNNAC | 4 cut(s) | 250, 361, 1108, 1549 |
| Hpy99I | CGWCG | 1 cut(s) | 596 |
| HpyAV | CCTTC | 4 cut(s) | 655, 695, 796, 922 |
| HpyCH4III | ACNGT | 8 cut(s) | 172, 353, 433, 635, 1080, 1373, 1570, 1734 |
| HpyCH4IV | ACGT | 4 cut(s) | 831, 894, 1092, 1224 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1059, 1152, 1387 |
| HpyF3I | CTNAG | 4 cut(s) | 78, 422, 1344, 1530 |
| HpySE526I | ACGT | 4 cut(s) | 831, 894, 1092, 1224 |
| Hsp92I | GRCGYC | 2 cut(s) | 594, 768 |
| KroI | GCCGGC | 1 cut(s) | 313 |
| KroNI | GCCGGC | 1 cut(s) | 315 |
| Kzo9I | GATC | 5 cut(s) | 293, 425, 535, 1653, 1738 |
| LguI | GCTCTTC | 1 cut(s) | 728 |
| Lsp1109I | GCAGC | 5 cut(s) | 716, 1074, 1171, 1174, 1195 |
| MaeI | CTAG | 7 cut(s) | 905, 941, 1404, 1431, 1610, 1662, 1694 |
| MaeII | ACGT | 4 cut(s) | 831, 894, 1092, 1224 |
| MaeIII | GTNAC | 5 cut(s) | 166, 322, 347, 895, 1643 |
| MalI | GATC | 5 cut(s) | 295, 427, 537, 1655, 1740 |
| MboI | GATC | 5 cut(s) | 293, 425, 535, 1653, 1738 |
| MflI | RGATCY | 3 cut(s) | 535, 1653, 1738 |
| MhlI | GDGCHC | 2 cut(s) | 723, 1345 |
| MlsI | TGGCCA | 1 cut(s) | 1275 |
| MluCI | AATT | 3 cut(s) | 1330, 1477, 1599 |
| MluNI | TGGCCA | 1 cut(s) | 1275 |
| MmeI | TCCRAC | 1 cut(s) | 615 |
| MnlI | CCTC | 9 cut(s) | 204, 205, 380, 569, 758, 983, 1467, 1591, 1737 |
| Mox20I | TGGCCA | 1 cut(s) | 1275 |
| Mph1103I | ATGCAT | 1 cut(s) | 1641 |
| MroNI | GCCGGC | 1 cut(s) | 313 |
| MscI | TGGCCA | 1 cut(s) | 1275 |
| MseI | TTAA | 7 cut(s) | 174, 231, 240, 606, 1067, 1319, 1398 |
| MslI | CAYNNNNRTG | 2 cut(s) | 273, 1467 |
| Msp20I | TGGCCA | 1 cut(s) | 1275 |
| MspA1I | CMGCKG | 1 cut(s) | 1348 |
| MspI | CCGG | 5 cut(s) | 189, 297, 314, 815, 1584 |
| MspR9I | CCNGG | 8 cut(s) | 103, 189, 298, 557, 618, 815, 816, 1623 |
| Mva1269I | GAATGC | 2 cut(s) | 535, 936 |
| MvaI | CCWGG | 4 cut(s) | 103, 557, 618, 1623 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1059, 1152, 1387 |
| NaeI | GCCGGC | 1 cut(s) | 315 |
| NciI | CCSGG | 4 cut(s) | 189, 298, 815, 816 |
| NdeI | CATATG | 1 cut(s) | 319 |
| NdeII | GATC | 5 cut(s) | 293, 425, 535, 1653, 1738 |
| NgoMIV | GCCGGC | 1 cut(s) | 313 |
| NlaIV | GGNNCC | 5 cut(s) | 391, 442, 910, 1141, 1189 |
| NmuCI | GTSAC | 3 cut(s) | 322, 895, 1643 |
| NsiI | ATGCAT | 1 cut(s) | 1641 |
| NspI | RCATGY | 3 cut(s) | 115, 407, 1752 |
| PaeI | GCATGC | 1 cut(s) | 407 |
| PagI | TCATGA | 1 cut(s) | 876 |
| PciI | ACATGT | 2 cut(s) | 111, 1748 |
| PciSI | GCTCTTC | 1 cut(s) | 728 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 289 |
| PctI | GAATGC | 2 cut(s) | 535, 936 |
| PdiI | GCCGGC | 1 cut(s) | 315 |
| PfeI | GAWTC | 2 cut(s) | 280, 1473 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 270, 464 |
| PfoI | TCCNGGA | 1 cut(s) | 187 |
| PkrI | GCNGC | 5 cut(s) | 706, 1064, 1161, 1164, 1210 |
| Ppu21I | YACGTR | 1 cut(s) | 895 |
| PpuMI | RGGWCCY | 1 cut(s) | 1187 |
| PscI | ACATGT | 2 cut(s) | 111, 1748 |
| PshBI | ATTAAT | 1 cut(s) | 606 |
| Psp124BI | GAGCTC | 1 cut(s) | 723 |
| Psp1406I | AACGTT | 2 cut(s) | 831, 1224 |
| Psp5II | RGGWCCY | 1 cut(s) | 1187 |
| Psp6I | CCWGG | 4 cut(s) | 101, 555, 616, 1621 |
| PspFI | CCCAGC | 4 cut(s) | 671, 1012, 1084, 1665 |
| PspGI | CCWGG | 4 cut(s) | 101, 555, 616, 1621 |
| PspN4I | GGNNCC | 5 cut(s) | 391, 442, 910, 1141, 1189 |
| PspPI | GGNCC | 7 cut(s) | 191, 303, 311, 440, 578, 620, 1187 |
| PspPPI | RGGWCCY | 1 cut(s) | 1187 |
| PstNI | CAGNNNCTG | 1 cut(s) | 539 |
| PsuI | RGATCY | 3 cut(s) | 535, 1653, 1738 |
| PvuII | CAGCTG | 1 cut(s) | 1348 |
| RsaI | GTAC | 5 cut(s) | 362, 504, 629, 947, 1375 |
| RsaNI | GTAC | 5 cut(s) | 361, 503, 628, 946, 1374 |
| RseI | CAYNNNNRTG | 2 cut(s) | 273, 1467 |
| SacI | GAGCTC | 1 cut(s) | 723 |
| SapI | GCTCTTC | 1 cut(s) | 728 |
| SaqAI | TTAA | 7 cut(s) | 174, 231, 240, 606, 1067, 1319, 1398 |
| SatI | GCNGC | 5 cut(s) | 705, 1063, 1160, 1163, 1209 |
| Sau3AI | GATC | 5 cut(s) | 293, 425, 535, 1653, 1738 |
| Sau96I | GGNCC | 7 cut(s) | 191, 303, 311, 440, 578, 620, 1187 |
| ScrFI | CCNGG | 8 cut(s) | 103, 189, 298, 557, 618, 815, 816, 1623 |
| SduI | GDGCHC | 2 cut(s) | 723, 1345 |
| SinI | GGWCC | 4 cut(s) | 191, 440, 620, 1187 |
| SmaI | CCCGGG | 1 cut(s) | 816 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 273, 1467 |
| SpeI | ACTAGT | 2 cut(s) | 1403, 1430 |
| SphI | GCATGC | 1 cut(s) | 407 |
| Sse9I | AATT | 3 cut(s) | 1330, 1477, 1599 |
| SsiI | CCGC | 4 cut(s) | 882, 957, 1060, 1587 |
| SspMI | CTAG | 7 cut(s) | 905, 941, 1404, 1431, 1610, 1662, 1694 |
| SstI | GAGCTC | 1 cut(s) | 723 |
| StyD4I | CCNGG | 8 cut(s) | 101, 187, 296, 555, 616, 813, 814, 1621 |
| StyI | CCWWGG | 1 cut(s) | 1183 |
| TaaI | ACNGT | 8 cut(s) | 172, 353, 433, 635, 1080, 1373, 1570, 1734 |
| TaiI | ACGT | 4 cut(s) | 834, 897, 1095, 1227 |
| TaqI | TCGA | 5 cut(s) | 292, 759, 789, 915, 1512 |
| TasI | AATT | 3 cut(s) | 1330, 1477, 1599 |
| TatI | WGTACW | 2 cut(s) | 360, 627 |
| TfiI | GAWTC | 2 cut(s) | 280, 1473 |
| Tru1I | TTAA | 7 cut(s) | 174, 231, 240, 606, 1067, 1319, 1398 |
| Tru9I | TTAA | 7 cut(s) | 174, 231, 240, 606, 1067, 1319, 1398 |
| TscAI | CASTG | 3 cut(s) | 984, 1252, 1342 |
| TseFI | GTSAC | 3 cut(s) | 322, 895, 1643 |
| TseI | GCWGC | 5 cut(s) | 704, 1062, 1159, 1162, 1208 |
| Tsp45I | GTSAC | 3 cut(s) | 322, 895, 1643 |
| TspDTI | ATGAA | 7 cut(s) | 9, 46, 405, 500, 1451, 1457, 1624 |
| TspGWI | ACGGA | 1 cut(s) | 1383 |
| TspMI | CCCGGG | 1 cut(s) | 814 |
| TspRI | CASTG | 3 cut(s) | 984, 1252, 1342 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 270, 464 |
| VpaK11BI | GGWCC | 4 cut(s) | 191, 440, 620, 1187 |
| VspI | ATTAAT | 1 cut(s) | 606 |
| XbaI | TCTAGA | 1 cut(s) | 940 |
| XceI | RCATGY | 3 cut(s) | 115, 407, 1752 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1190 |
| XmaI | CCCGGG | 1 cut(s) | 814 |
| XspI | CTAG | 7 cut(s) | 905, 941, 1404, 1431, 1610, 1662, 1694 |
| Zsp2I | ATGCAT | 1 cut(s) | 1641 |