pycom03g08590

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000610
Physical Location & Seq
Forward (+)
6955476 .. 6959343
3868 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g08590.6

Sequence Viewer

Length: 1845 bp
ATGAAATCTCTCACTTTCTTCTCCACCCGCCCCCCCCCCCCCCCCCCCAAAAAAAAAAAAAACAAAGAGAGAGAGAGAGAGAGAGAGAGTAAAATGGGAGTTCCTCTTCTTTCGTCAGCAACACTTCTTAGACTCTTCCTCTTTTCTCTCATTACGTTGTGTCTCATTCCTCAGCATGCTCTTGCAAGCATAACGAGGCACTACAAATTTGATATCAAACATCAAAATGTGACAAGACTATGCCACACAAAGAGCATTGTAACAGTAAACGGGCAATTTCCAGGGCCTCGAATTGTTGCTCGGGAGGGTGATCACGTCCTTATTAAAGTGGTTAATCATGTTCAAAACAACATTTCCATCCACTGGCATGGCATTCGACAGCTTCGATCAGGGTGGGCTGATGGACCTGCATATGTTACCCAATGTCCTATACAAACAGGCAACAATTATGTCTACAACTTCACCATTACAGGCCAAAGAGGCACTCTTTTCTGGCATGCTCATATATCATGGCTTAGATCAACTGTTTATGGTCCCTTGATCATACTTCCCAAGCGCGGTGTACCTTACCCATTTGCTAAGCCCTACAAGGAGATTCCGATCATCTTCGGAGAGTGGTGGAATGTAAACCCTGAAGCTGTTATTAGCCAAGCCTTGCAAACAGGTGGAGGGCCAAACGTCTCTGATGCTTATACCATCAATGGACTTCCAGGGCCCTTTTACAATTGTTCTGCCAAAGACACATTCAAGCTGAAGGTTCAATCTGGGAAGACTTACCTTCTCCGCCTAATCAACGCTGCGCTCAATGATGAGCTCTTCTTTAGCATTGCAAACCACACCCTCAAAGTGGTAGAAGCTGATGCGATCTATGTCAAACCCTTTGAGACTGACACAATTCTGATTGCCCCGGGGCAGACTACAAATGTTCTGCTCAAGACCAAAGCAAATTTCCCCAATGCCACATTTTTCATGACCGCTAGGCCATACGCGACTGGACTAGGCACCTTTGACAACTCCACTGTTGCTGCTATTTTAGAGTATGAATCACCCTCCAAAACCCTCCATTCAACCCTCCCAATGAAGAAGCTTCCGCTCTTCAAACCAGTTCTGCCTGCTCTAAATGACACATCATTTGCAACAAAGTTCAGCAACAAACTTCGTAGCTTAGCCAATGCACAATTCCCTGCTAATGTCCCCCAAAAGGTTGACAAACACTTTTTCTTCACAATAGGCCTTGGAACCAACCCATGCAGCAACAGTAACCAAACCTGCCAGGGACCTAATGGGACAATGTTTGCGGCTTCAGTCAATAACGTTTCTTTCGCAATGCCAACCACCGCTCTACTTCAAGCCCACTTCACGGGGCAATCAAATGCGGTTTACAGCCCTTACTTCCCAAGCAGTCCTTTAATTCCATTTAACTATACAGGCACCCCACCTAATAATACAATGGTTGGCAATGGTACAAAGCTAATGGTACTTCCATTCAACACTAGTGTGGAGTTGGTGATGCAGGACACAAGCATTCTGGGCGTCGAAAGCCACCCTCTTCATCTTCATGGATTTAATTTCTTTGTTGTTGGACAAGGGTTCGGGAACTTTGACCGGAATAAGGACCCTGCAAACTTCAATTTGGTCGACCCTGTTGAAAGAAATACTGTGGGTGTGCCATCTGGTGGATGGGTTGCAATACGATTCCTAGCAGATAACCCCGGTGTATGGTTCATGCATTGCCATTTGGAGATCCATACTAGTTGGGGCTTGAAGATGGCTTGGCTAGTCCTAGATGGAAAGCTTCCCAACCAAAAGTTGCTTCCTCCCCCAGCAGATCTTCCCACTTGTTAA

Protein Analysis

615

Amino Acids

68.06

Weight (kDa)

9.52

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000610)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60020 AT5G60020 AT5G60020
fragaria_vesca FvH4_2g09062 FvH4_2g09062 FvH4_3g05920 FvH4_3g34100 FvH4_3g34310 FvH4_3g34340 FvH4_6g13390
malus_domestica MD00G1015000.v1.1 MD03G1106500.v1.1 MD03G1106600.v1.1 MD04G1131500.v1.1 MD11G1118900.v1.1 MD11G1119000.v1.1 MD12G1144600.v1.1
prunus_persica Prupe.6G089100_v2.0.a1 Prupe.6G089300_v2.0.a1 Prupe.6G258000_v2.0.a1
pyrus_communis pycom03g08590 pycom10g02940 pycom11g09990 pycom11g10060
rosa_chinensis RchiOBHm_Chr2g0154491 RchiOBHm_Chr3g0466271 RchiOBHm_Chr5g0061891 RchiOBHm_Chr5g0061911 RchiOBHm_Chr5g0061951 RchiOBHm_Chr5g0061961 RchiOBHm_Chr6g0264371
rosa_laevigata RLG00000024591 RLG00000035514 RLG00000035516 RLG00000035518 RLG00000035519
rosa_multiflora Rmu_sc0003480.1_g000021 Rmu_sc0011641.1_g000002 Rmu_sc0011641.1_g000009
rosa_roxburghii Rroxscaffold_1G00018790 Rroxscaffold_1G00018820 Rroxscaffold_1G00018850 Rroxscaffold_2G00122520 Rroxscaffold_2G00122540 Rroxscaffold_6G00414660
rosa_rugosa Rorug03G0080700 Rorug04G0180900 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345100 Rorug05G0345200 Rorug06G0011800
rosa_samantha Rh3DG148900 Rh5AG405700 Rh5AG406100 Rh5AG406200 Rh5BG419200 Rh5BG419400 Rh5CG443100 Rh5CG443300 Rh5CG443500 Rh5CG443600 Rh5DG432700 Rh5DG434900 Rh6BG128700 Rh6CG127600 Rh6DG114200 Rh6DG114300
rosa_wichuraiana Rw3G011980 Rw5G038100 Rw5G038140 Rw5G038160 Rw5G038200 Rw5G038210 Rw5G038250 Rw5G038270 Rw5G038290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 415, 1277
AccB1I GGYRCC 2 cut(s) 1001, 1430
AccB7I CCANNNNNTGG 2 cut(s) 363, 1676
AccBSI CCGCTC 2 cut(s) 1093, 1340
AccI GTMKAC 2 cut(s) 453, 1638
AccII CGCG 2 cut(s) 558, 989
AciI CCGC 8 cut(s) 28, 558, 784, 975, 1091, 1298, 1338, 1376
AclI AACGTT 1 cut(s) 1314
AclWI GGATC 1 cut(s) 1738
AcsI RAATTY 2 cut(s) 206, 946
AcuI CTGAAG 3 cut(s) 654, 773, 1287
AcyI GRCGYC 1 cut(s) 1533
AfaI GTAC 3 cut(s) 564, 1465, 1479
AfiI CCNNNNNNNGG 8 cut(s) 363, 557, 847, 1201, 1360, 1612, 1676, 1719
AhlI ACTAGT 2 cut(s) 1493, 1751
AjiI CACGTC 1 cut(s) 316
AjnI CCWGG 3 cut(s) 280, 709, 1272
AjuI GAANNNNNNNTTGG 8 cut(s) 728, 760, 932, 964, 1163, 1195, 1797, 1829
AleI CACNNNNGTG 1 cut(s) 1496
AluBI AGCT 9 cut(s) 382, 638, 751, 814, 857, 1087, 1164, 1471, 1795
AluI AGCT 9 cut(s) 382, 638, 751, 814, 857, 1087, 1164, 1471, 1795
Alw21I GWGCWC 1 cut(s) 816
Alw26I GTCTC 3 cut(s) 167, 685, 878
AlwI GGATC 1 cut(s) 1738
Ama87I CYCGRG 2 cut(s) 300, 907
AoxI GGCC 6 cut(s) 284, 472, 671, 713, 980, 1231
ApaI GGGCCC 1 cut(s) 717
ApeKI GCWGC 3 cut(s) 797, 1025, 1251
ApoI RAATTY 2 cut(s) 206, 946
ArsI GACNNNNNNTTYG 2 cut(s) 1115, 1147
AspLEI GCGC 2 cut(s) 558, 802
AspS9I GGNCC 8 cut(s) 284, 404, 533, 671, 713, 714, 1277, 1615
AsuC2I CCSGG 3 cut(s) 908, 909, 1713
AsuHPI GGTGA 4 cut(s) 320, 454, 1038, 1519
AvaI CYCGRG 2 cut(s) 300, 907
AvaII GGWCC 4 cut(s) 404, 533, 1277, 1615
BaeGI GKGCMC 1 cut(s) 717
BanI GGYRCC 2 cut(s) 1001, 1430
BanII GRGCYC 2 cut(s) 717, 816
BbsI GAAGAC 1 cut(s) 776
Bbv12I GWGCWC 1 cut(s) 816
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 3 cut(s) 784, 1012, 1263
BccI CCATC 7 cut(s) 365, 395, 704, 1674, 1678, 1762, 1781
BcgI CGANNNNNNTGC 2 cut(s) 356, 390
BciT130I CCWGG 3 cut(s) 282, 711, 1274
BclI TGATCA 2 cut(s) 310, 540
BcnI CCSGG 3 cut(s) 908, 909, 1713
BcoDI GTCTC 3 cut(s) 167, 685, 878
BcuI ACTAGT 2 cut(s) 1493, 1751
BfaI CTAG 7 cut(s) 978, 998, 1494, 1700, 1752, 1778, 1784
BfuAI ACCTGC 2 cut(s) 415, 1277
BglI GCCNNNNNGGC 1 cut(s) 480
BglII AGATCT 1 cut(s) 1828
BisI GCNGC 4 cut(s) 798, 1026, 1252, 1299
BlpI GCTNAGC 2 cut(s) 579, 1165
BlsI GCNGC 4 cut(s) 799, 1027, 1253, 1300
Bme1390I CCNGG 6 cut(s) 282, 711, 908, 909, 1274, 1713
Bme18I GGWCC 4 cut(s) 404, 533, 1277, 1615
BmeT110I CYCGRG 2 cut(s) 300, 907
BmgBI CACGTC 1 cut(s) 316
BmgT120I GGNCC 8 cut(s) 284, 404, 533, 671, 713, 714, 1277, 1615
BmiI GGNNCC 7 cut(s) 535, 715, 1003, 1240, 1278, 1432, 1617
BmrFI CCNGG 6 cut(s) 282, 711, 908, 909, 1274, 1713
BmsI GCATC 3 cut(s) 676, 850, 1500
BpiI GAAGAC 1 cut(s) 776
Bpu10I CCTNAGC 1 cut(s) 171
Bpu1102I GCTNAGC 2 cut(s) 579, 1165
BpuEI CTTGAG 1 cut(s) 917
BpuMI CCSGG 3 cut(s) 908, 909, 1713
BsaBI GATNNNNATC 1 cut(s) 599
BsaHI GRCGYC 1 cut(s) 1533
BsaJI CCNNGG 8 cut(s) 281, 710, 906, 907, 908, 1234, 1273, 1711
BsaWI WCCGGW 1 cut(s) 1605
Bsc4I CCNNNNNNNGG 8 cut(s) 363, 557, 847, 1201, 1360, 1612, 1676, 1719
Bse1I ACTGG 3 cut(s) 368, 997, 1103
Bse3DI GCAATG 4 cut(s) 825, 1332, 1465, 1729
Bse8I GATNNNNATC 1 cut(s) 599
BseBI CCWGG 3 cut(s) 282, 711, 1274
BseDI CCNNGG 8 cut(s) 281, 710, 906, 907, 908, 1234, 1273, 1711
BseGI GGATG 2 cut(s) 357, 1685
BseJI GATNNNNATC 1 cut(s) 599
BseLI CCNNNNNNNGG 8 cut(s) 363, 557, 847, 1201, 1360, 1612, 1676, 1719
BseMI GCAATG 4 cut(s) 825, 1332, 1465, 1729
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 3 cut(s) 368, 997, 1103
BseSI GKGCMC 1 cut(s) 717
BseXI GCAGC 3 cut(s) 784, 1012, 1263
BseYI CCCAGC 1 cut(s) 1822
Bsh1236I CGCG 2 cut(s) 558, 989
BshFI GGCC 6 cut(s) 286, 474, 673, 715, 982, 1233
BshNI GGYRCC 2 cut(s) 1001, 1430
BsiHKAI GWGCWC 1 cut(s) 816
BsiHKCI CYCGRG 2 cut(s) 300, 907
BsiSI CCGG 3 cut(s) 908, 1606, 1713
BslFI GGGAC 4 cut(s) 519, 1178, 1290, 1300
BslI CCNNNNNNNGG 8 cut(s) 363, 557, 847, 1201, 1360, 1612, 1676, 1719
BsmAI GTCTC 3 cut(s) 167, 685, 878
BsmBI CGTCTC 1 cut(s) 685
BsmFI GGGAC 4 cut(s) 519, 1178, 1290, 1300
BsmI GAATGC 2 cut(s) 372, 1524
BsnI GGCC 6 cut(s) 286, 474, 673, 715, 982, 1233
BsoBI CYCGRG 2 cut(s) 300, 907
Bsp120I GGGCCC 1 cut(s) 713
Bsp1286I GDGCHC 2 cut(s) 717, 816
Bsp143I GATC 8 cut(s) 310, 386, 518, 540, 600, 864, 1743, 1828
Bsp1720I GCTNAGC 2 cut(s) 579, 1165
BspACI CCGC 8 cut(s) 28, 558, 784, 975, 1091, 1298, 1338, 1376
BspANI GGCC 6 cut(s) 286, 474, 673, 715, 982, 1233
BspCNI CTCAG 1 cut(s) 184
BspFNI CGCG 2 cut(s) 558, 989
BspHI TCATGA 1 cut(s) 969
BspLI GGNNCC 7 cut(s) 535, 715, 1003, 1240, 1278, 1432, 1617
BspMI ACCTGC 2 cut(s) 415, 1277
BspPI GGATC 1 cut(s) 1738
BspQI GCTCTTC 2 cut(s) 821, 1100
BspT107I GGYRCC 2 cut(s) 1001, 1430
BsrBI CCGCTC 2 cut(s) 1093, 1340
BsrDI GCAATG 4 cut(s) 825, 1332, 1465, 1729
BsrI ACTGG 3 cut(s) 368, 997, 1103
BssECI CCNNGG 8 cut(s) 281, 710, 906, 907, 908, 1234, 1273, 1711
BssMI GATC 8 cut(s) 310, 386, 518, 540, 600, 864, 1743, 1828
BssNI GRCGYC 1 cut(s) 1533
BssT1I CCWWGG 1 cut(s) 1234
Bst2UI CCWGG 3 cut(s) 282, 711, 1274
Bst4CI ACNGT 5 cut(s) 265, 526, 1021, 1259, 1660
Bst6I CTCTTC 5 cut(s) 111, 140, 821, 1100, 1554
BstACI GRCGYC 1 cut(s) 1533
BstC8I GCNNGC 4 cut(s) 177, 187, 498, 1113
BstDEI CTNAG 5 cut(s) 128, 171, 515, 579, 1165
BstF5I GGATG 2 cut(s) 357, 1685
BstFNI CGCG 2 cut(s) 558, 989
BstHHI GCGC 2 cut(s) 558, 802
BstKTI GATC 8 cut(s) 313, 389, 521, 543, 603, 867, 1746, 1831
BstMAI GTCTC 3 cut(s) 167, 685, 878
BstMBI GATC 8 cut(s) 310, 386, 518, 540, 600, 864, 1743, 1828
BstMWI GCNNNNNNNGC 3 cut(s) 480, 1530, 1539
BstNI CCWGG 3 cut(s) 282, 711, 1274
BstNSI RCATGY 2 cut(s) 179, 500
BstSCI CCNGG 6 cut(s) 280, 709, 906, 907, 1272, 1711
BstSLI GKGCMC 1 cut(s) 717
BstUI CGCG 2 cut(s) 558, 989
BstV1I GCAGC 3 cut(s) 784, 1012, 1263
BstV2I GAAGAC 1 cut(s) 776
BstX2I RGATCY 2 cut(s) 1743, 1828
BstXI CCANNNNNNTGG 1 cut(s) 368
BstYI RGATCY 2 cut(s) 1743, 1828
BsuRI GGCC 6 cut(s) 286, 474, 673, 715, 982, 1233
BtrI CACGTC 1 cut(s) 316
BtsCI GGATG 2 cut(s) 357, 1685
BtsIMutI CAGTG 2 cut(s) 361, 1017
BveI ACCTGC 2 cut(s) 415, 1277
Cac8I GCNNGC 4 cut(s) 177, 187, 498, 1113
CciI TCATGA 1 cut(s) 969
CfoI GCGC 2 cut(s) 558, 802
Cfr13I GGNCC 8 cut(s) 284, 404, 533, 671, 713, 714, 1277, 1615
Cfr9I CCCGGG 1 cut(s) 907
CseI GACGC 1 cut(s) 1522
Csp6I GTAC 3 cut(s) 563, 1464, 1478
CviAII CATG 9 cut(s) 176, 338, 368, 497, 510, 970, 1248, 1559, 1726
CviQI GTAC 3 cut(s) 563, 1464, 1478
DdeI CTNAG 5 cut(s) 128, 171, 515, 579, 1165
DpnI GATC 8 cut(s) 312, 388, 520, 542, 602, 866, 1745, 1830
DpnII GATC 8 cut(s) 310, 386, 518, 540, 600, 864, 1743, 1828
Eam1104I CTCTTC 5 cut(s) 111, 140, 821, 1100, 1554
EarI CTCTTC 5 cut(s) 111, 140, 821, 1100, 1554
EciI GGCGGA 1 cut(s) 773
Ecl136II GAGCTC 1 cut(s) 814
Eco130I CCWWGG 1 cut(s) 1234
Eco147I AGGCCT 1 cut(s) 1233
Eco24I GRGCYC 2 cut(s) 717, 816
Eco32I GATATC 1 cut(s) 214
Eco47I GGWCC 4 cut(s) 404, 533, 1277, 1615
Eco53kI GAGCTC 1 cut(s) 814
Eco57I CTGAAG 3 cut(s) 654, 773, 1287
Eco88I CYCGRG 2 cut(s) 300, 907
EcoICRI GAGCTC 1 cut(s) 814
EcoO109I RGGNCCY 5 cut(s) 284, 713, 714, 1277, 1615
EcoRII CCWGG 3 cut(s) 280, 709, 1272
EcoRV GATATC 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 1234
EcoT22I ATGCAT 1 cut(s) 1731
EcoT38I GRGCYC 2 cut(s) 717, 816
ErhI CCWWGG 1 cut(s) 1234
Esp3I CGTCTC 1 cut(s) 685
FaeI CATG 9 cut(s) 179, 341, 371, 500, 513, 973, 1251, 1562, 1729
FalI AAGNNNNNCTT 2 cut(s) 1390, 1422
FaqI GGGAC 4 cut(s) 519, 1178, 1290, 1300
FatI CATG 9 cut(s) 175, 337, 367, 496, 509, 969, 1247, 1558, 1725
FauI CCCGC 1 cut(s) 35
FauNDI CATATG 1 cut(s) 412
FbaI TGATCA 2 cut(s) 310, 540
FblI GTMKAC 2 cut(s) 453, 1638
Fnu4HI GCNGC 4 cut(s) 798, 1026, 1252, 1299
FokI GGATG 2 cut(s) 344, 1692
FriOI GRGCYC 2 cut(s) 717, 816
Fsp4HI GCNGC 4 cut(s) 798, 1026, 1252, 1299
FspBI CTAG 7 cut(s) 978, 998, 1494, 1700, 1752, 1778, 1784
GlaI GCGC 2 cut(s) 557, 801
GluI GCNGC 4 cut(s) 798, 1026, 1252, 1299
GsaI CCCAGC 1 cut(s) 1826
HaeIII GGCC 6 cut(s) 286, 474, 673, 715, 982, 1233
HapII CCGG 3 cut(s) 908, 1606, 1713
HgaI GACGC 1 cut(s) 1522
HhaI GCGC 2 cut(s) 558, 802
Hin1I GRCGYC 1 cut(s) 1533
Hin1II CATG 9 cut(s) 179, 341, 371, 500, 513, 973, 1251, 1562, 1729
Hin6I GCGC 2 cut(s) 556, 800
HinP1I GCGC 2 cut(s) 556, 800
HincII GTYRAC 2 cut(s) 1207, 1639
HindII GTYRAC 2 cut(s) 1207, 1639
HindIII AAGCTT 2 cut(s) 1085, 1793
HinfI GANTC 4 cut(s) 132, 595, 1043, 1695
HpaII CCGG 3 cut(s) 908, 1606, 1713
HphI GGTGA 4 cut(s) 320, 454, 1038, 1519
Hpy166II GTNNAC 7 cut(s) 268, 454, 563, 628, 1207, 1381, 1639
Hpy188I TCNGA 4 cut(s) 600, 611, 685, 900
Hpy188III TCNNGA 4 cut(s) 302, 934, 970, 1594
Hpy8I GTNNAC 7 cut(s) 268, 454, 563, 628, 1207, 1381, 1639
Hpy99I CGWCG 1 cut(s) 1538
HpyAV CCTTC 2 cut(s) 748, 788
HpyCH4III ACNGT 5 cut(s) 265, 526, 1021, 1259, 1660
HpyCH4IV ACGT 4 cut(s) 155, 315, 678, 1314
HpyF10VI GCNNNNNNNGC 3 cut(s) 480, 1530, 1539
HpyF3I CTNAG 5 cut(s) 128, 171, 515, 579, 1165
HpySE526I ACGT 4 cut(s) 155, 315, 678, 1314
Hsp92I GRCGYC 1 cut(s) 1533
Hsp92II CATG 9 cut(s) 179, 341, 371, 500, 513, 973, 1251, 1562, 1729
HspAI GCGC 2 cut(s) 556, 800
Ksp22I TGATCA 2 cut(s) 310, 540
Kzo9I GATC 8 cut(s) 310, 386, 518, 540, 600, 864, 1743, 1828
LguI GCTCTTC 2 cut(s) 821, 1100
Lsp1109I GCAGC 3 cut(s) 784, 1012, 1263
LweI GCATC 3 cut(s) 676, 850, 1500
MaeI CTAG 7 cut(s) 978, 998, 1494, 1700, 1752, 1778, 1784
MaeII ACGT 4 cut(s) 155, 315, 678, 1314
MaeIII GTNAC 4 cut(s) 229, 259, 415, 1259
MalI GATC 8 cut(s) 312, 388, 520, 542, 602, 866, 1745, 1830
MbiI CCGCTC 2 cut(s) 1093, 1340
MboI GATC 8 cut(s) 310, 386, 518, 540, 600, 864, 1743, 1828
MfeI CAATTG 1 cut(s) 724
MflI RGATCY 2 cut(s) 1743, 1828
MhlI GDGCHC 2 cut(s) 717, 816
MlyI GAGTC 1 cut(s) 126
MmeI TCCRAC 1 cut(s) 1561
Mph1103I ATGCAT 1 cut(s) 1731
MseI TTAA 6 cut(s) 324, 333, 1409, 1419, 1566, 1843
MslI CAYNNNNRTG 4 cut(s) 225, 366, 1496, 1557
MspI CCGG 3 cut(s) 908, 1606, 1713
MspR9I CCNGG 6 cut(s) 282, 711, 908, 909, 1274, 1713
MunI CAATTG 1 cut(s) 724
Mva1269I GAATGC 2 cut(s) 372, 1524
MvaI CCWGG 3 cut(s) 282, 711, 1274
MvnI CGCG 2 cut(s) 558, 989
MwoI GCNNNNNNNGC 3 cut(s) 480, 1530, 1539
NciI CCSGG 3 cut(s) 908, 909, 1713
NdeI CATATG 1 cut(s) 412
NdeII GATC 8 cut(s) 310, 386, 518, 540, 600, 864, 1743, 1828
NlaIII CATG 9 cut(s) 179, 341, 371, 500, 513, 973, 1251, 1562, 1729
NlaIV GGNNCC 7 cut(s) 535, 715, 1003, 1240, 1278, 1432, 1617
NmuCI GTSAC 1 cut(s) 229
NsiI ATGCAT 1 cut(s) 1731
NspI RCATGY 2 cut(s) 179, 500
OliI CACNNNNGTG 1 cut(s) 1496
PaeI GCATGC 2 cut(s) 179, 500
PagI TCATGA 1 cut(s) 969
PceI AGGCCT 1 cut(s) 1233
PciSI GCTCTTC 2 cut(s) 821, 1100
PcsI WCGNNNNNNNCGW 1 cut(s) 382
PctI GAATGC 2 cut(s) 372, 1524
PfeI GAWTC 3 cut(s) 595, 1043, 1695
PflMI CCANNNNNTGG 2 cut(s) 363, 1676
PkrI GCNGC 4 cut(s) 799, 1027, 1253, 1300
PleI GAGTC 1 cut(s) 126
PpsI GAGTC 1 cut(s) 126
PpuMI RGGWCCY 2 cut(s) 1277, 1615
Psp124BI GAGCTC 1 cut(s) 816
Psp1406I AACGTT 1 cut(s) 1314
Psp5II RGGWCCY 2 cut(s) 1277, 1615
Psp6I CCWGG 3 cut(s) 280, 709, 1272
PspFI CCCAGC 1 cut(s) 1822
PspGI CCWGG 3 cut(s) 280, 709, 1272
PspN4I GGNNCC 7 cut(s) 535, 715, 1003, 1240, 1278, 1432, 1617
PspOMI GGGCCC 1 cut(s) 713
PspPI GGNCC 8 cut(s) 284, 404, 533, 671, 713, 714, 1277, 1615
PspPPI RGGWCCY 2 cut(s) 1277, 1615
PsuI RGATCY 2 cut(s) 1743, 1828
RsaI GTAC 3 cut(s) 564, 1465, 1479
RsaNI GTAC 3 cut(s) 563, 1464, 1478
RseI CAYNNNNRTG 4 cut(s) 225, 366, 1496, 1557
SacI GAGCTC 1 cut(s) 816
SalI GTCGAC 1 cut(s) 1637
SapI GCTCTTC 2 cut(s) 821, 1100
SaqAI TTAA 6 cut(s) 324, 333, 1409, 1419, 1566, 1843
SatI GCNGC 4 cut(s) 798, 1026, 1252, 1299
Sau3AI GATC 8 cut(s) 310, 386, 518, 540, 600, 864, 1743, 1828
Sau96I GGNCC 8 cut(s) 284, 404, 533, 671, 713, 714, 1277, 1615
SchI GAGTC 1 cut(s) 126
ScrFI CCNGG 6 cut(s) 282, 711, 908, 909, 1274, 1713
SduI GDGCHC 2 cut(s) 717, 816
SfaNI GCATC 3 cut(s) 676, 850, 1500
SinI GGWCC 4 cut(s) 404, 533, 1277, 1615
SmaI CCCGGG 1 cut(s) 909
SmiMI CAYNNNNRTG 4 cut(s) 225, 366, 1496, 1557
SmlI CTYRAG 1 cut(s) 932
SmoI CTYRAG 1 cut(s) 932
SpeI ACTAGT 2 cut(s) 1493, 1751
SphI GCATGC 2 cut(s) 179, 500
SseBI AGGCCT 1 cut(s) 1233
SsiI CCGC 8 cut(s) 28, 558, 784, 975, 1091, 1298, 1338, 1376
SspMI CTAG 7 cut(s) 978, 998, 1494, 1700, 1752, 1778, 1784
SstI GAGCTC 1 cut(s) 816
StuI AGGCCT 1 cut(s) 1233
StyD4I CCNGG 6 cut(s) 280, 709, 906, 907, 1272, 1711
StyI CCWWGG 1 cut(s) 1234
TaaI ACNGT 5 cut(s) 265, 526, 1021, 1259, 1660
TaiI ACGT 4 cut(s) 158, 318, 681, 1317
TaqI TCGA 5 cut(s) 289, 376, 385, 1536, 1638
TauI GCSGC 1 cut(s) 1301
TfiI GAWTC 3 cut(s) 595, 1043, 1695
Tru1I TTAA 6 cut(s) 324, 333, 1409, 1419, 1566, 1843
Tru9I TTAA 6 cut(s) 324, 333, 1409, 1419, 1566, 1843
TscAI CASTG 2 cut(s) 368, 1024
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 3 cut(s) 797, 1025, 1251
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 7 cut(s) 17, 958, 1056, 1094, 1541, 1547, 1714
TspMI CCCGGG 1 cut(s) 907
TspRI CASTG 2 cut(s) 368, 1024
Van91I CCANNNNNTGG 2 cut(s) 363, 1676
VpaK11BI GGWCC 4 cut(s) 404, 533, 1277, 1615
XapI RAATTY 2 cut(s) 206, 946
XceI RCATGY 2 cut(s) 179, 500
XcmI CCANNNNNNNNNTGG 2 cut(s) 1280, 1677
XmaI CCCGGG 1 cut(s) 907
XmiI GTMKAC 2 cut(s) 453, 1638
XspI CTAG 7 cut(s) 978, 998, 1494, 1700, 1752, 1778, 1784
Zsp2I ATGCAT 1 cut(s) 1731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.