Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0000610

Physical Location & Seq

Genomic Coordinates Reverse (-)

67505188 .. 67508466

Gene Structure 3279 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G038250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1659 bp

ATGGGTGTAGCTCTTTTTTCATTTGCTAGGTTCTTTGTTCTCTCTTTCATCACATTGGGTCTCATTCCTCAGCTAGCTCCTGCAAGCATAACAAGGCACTACAAATTTGATGTACAACTGCAAAATGTGACAAGGCTGTGCCATACCAAAAGCATTGTAACCATAAACGGTCAATTTCCTGGGCCTCGAATTGTTGCTAGGGAGGGTGATCGCCTCCTCATTAAAGTTGTTAACCATGTCCGAAGCAACATTTCCATCCATTGGCATGGAATTCGACAGCTTCGAACTGGGTGGGCTGATGGTCCAGCATACATTACCCAATGCCCAATACAAACCGGCAACAGCTATGTGTACAACTTCACCATTGTAGGCCAGAGGGGCACTCTTTGGTGGCATGCTCACATTTCATGGCTAAGATTGACGCAATCAACCATCTATGGTCCCCTTATCATTCTTCCGGAGCGTGGTGTTCCTTATCCATTCACCAAACCCTATAAGGAAGCCTTGCAAACTGGGGGAGGACCAAATGTTTCTGATGCTTACACCATCAATGGACTCCCAGGGCCATTGTATAACTGCTCTGCCAAAGATACTTTCAAGCTAAAGGTTCAAGCTGGGAAGCCATACCTTCTGCGCCTAATCAATGCAGCGCTGAACGACGAGCTCTTCTTCAGCATTGCAAACCACACCCTCAAAGTGGTGGAAACTGATGCAGTTTACGTCAAACCCTTCGAGACCAACACCATTCTCATTGCCCCGGGACAAACCACAAATGTTCTTCTCAAAACAAAACCTAACTACCCAAACGCCACATTTTTCATGACTGCAAGGCCATACGTGACTGGTCTAGGCACCTTTGATAACTCCACTCTTGCTGGCATTTTAGAATACGAAACGACTACACTTCATTCAACCCTCCCAATGAACAAACTTCCACTCCACAAACCAGTTCTCCCAGTTCTAAATGACACATCGTTTGCAACAAAATTCGCCAACAAACTCCGTAGCTTGAACACTCCCCAGTTCCCTGCTAATGTTCCACAGAAAGTTGACAAGCATTTGTTCTTCACCGTAGGGCTTGGAACCAACCCATGTCGCCAGCAGCAACACAACAACCAGACTGCCAGGGACCGAATGGAACCATGTTTGCTGCTTCACAACAACATTTCTTTCACTATGCCAAACACCGCCTTACTCCAATCCCACTTCTTTGGCCAATCCAATGGGGTTTATTCCCCTAATTTTCCAATCAGTCCTTTAATTCCATTTAATTACACTGGAGCCCAGCTGAACAATACTAATGTGGGCAATGGTACTAAGCTAATGGTGCTTCCCTTTAACACTAGTGTGGAGTTGGTGTTGCAGGACACTAGTATTCTTGGTGCTGAAAGTCATCCTCTTCATCTTCATGGCTTTAATTTCTTTGTTGACCCTAGGAAGTTCAATCTGATTGACCCTGTTGAGAGAAACACCGTGGGTGTGCCCTCCGGCGGGTGGGTTGCTATTCGGTTTCTAGCAGATAACCCGGGTGTGTGGTTCATGCATTGCCACCTTGAGATCCATACCAGCTGGGGCCTGAAGATGGCTTGGGTAGTCTTAGATGGAAAGCTTTCCAATCAAAAGTTGCTTCCTCCGCCACTGGATCTGCCCAAATGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006725 GO:0008150 GO:0008152 GO:0009058 GO:0009698 GO:0009699 GO:0009808 GO:0009809 GO:0009987 GO:0016491 GO:0016722 GO:0019438 GO:0019748 GO:0044237 GO:0044249 GO:0044550 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

552

Amino Acids

61.61

Weight (kDa)

9.49

Isoelectric Point (pI)

31.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu-oxidase_3	PF07732	38 - 154	4.9e-43	Multicopper oxidase
Cu-oxidase	PF00394	163 - 298	4.1e-37	Multicopper oxidase
Cu-oxidase_2	PF07731	422 - 534	7.2e-34	Multicopper oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000610)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60020 AT5G60020 AT5G60020
fragaria_vesca	FvH4_2g09062 FvH4_2g09062 FvH4_3g05920 FvH4_3g34100 FvH4_3g34310 FvH4_3g34340 FvH4_6g13390
malus_domestica	MD00G1015000.v1.1 MD03G1106500.v1.1 MD03G1106600.v1.1 MD04G1131500.v1.1 MD11G1118900.v1.1 MD11G1119000.v1.1 MD12G1144600.v1.1
prunus_persica	Prupe.6G089100_v2.0.a1 Prupe.6G089300_v2.0.a1 Prupe.6G258000_v2.0.a1
pyrus_communis	pycom03g08590 pycom10g02940 pycom11g09990 pycom11g10060
rosa_chinensis	RchiOBHm_Chr2g0154491 RchiOBHm_Chr3g0466271 RchiOBHm_Chr5g0061891 RchiOBHm_Chr5g0061911 RchiOBHm_Chr5g0061951 RchiOBHm_Chr5g0061961 RchiOBHm_Chr6g0264371
rosa_laevigata	RLG00000024591 RLG00000035514 RLG00000035516 RLG00000035518 RLG00000035519
rosa_multiflora	Rmu_sc0003480.1_g000021 Rmu_sc0011641.1_g000002 Rmu_sc0011641.1_g000009
rosa_roxburghii	Rroxscaffold_1G00018790 Rroxscaffold_1G00018820 Rroxscaffold_1G00018850 Rroxscaffold_2G00122520 Rroxscaffold_2G00122540 Rroxscaffold_6G00414660
rosa_rugosa	Rorug03G0080700 Rorug04G0180900 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345100 Rorug05G0345200 Rorug06G0011800
rosa_samantha	Rh3DG148900 Rh5AG405700 Rh5AG406100 Rh5AG406200 Rh5BG419200 Rh5BG419400 Rh5CG443100 Rh5CG443300 Rh5CG443500 Rh5CG443600 Rh5DG432700 Rh5DG434900 Rh6BG128700 Rh6CG127600 Rh6DG114200 Rh6DG114300
rosa_wichuraiana	Rw3G011980 Rw5G038100 Rw5G038140 Rw5G038160 Rw5G038200 Rw5G038210 Rw5G038250 Rw5G038270 Rw5G038290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	851
AccB7I	CCANNNNNTGG	1 cut(s)	261
AccIII	TCCGGA	1 cut(s)	457
AciI	CCGC	3 cut(s)	1188, 1491, 1634
AclWI	GGATC	2 cut(s)	1552, 1650
AcoI	YGGCCR	1 cut(s)	1213
AcsI	RAATTY	3 cut(s)	104, 270, 986
AcuI	CTGAAG	2 cut(s)	655, 1598
AfaI	GTAC	3 cut(s)	114, 353, 1315
AfeI	AGCGCT	1 cut(s)	651
AfiI	CCNNNNNNNGG	7 cut(s)	261, 464, 697, 1490, 1491, 1494, 1582
AgsI	TTSAA	5 cut(s)	598, 611, 912, 1012, 1444
AhlI	ACTAGT	2 cut(s)	1343, 1370
AjnI	CCWGG	3 cut(s)	178, 559, 1124
AjuI	GAANNNNNNNTTGG	2 cut(s)	578, 610
AleI	CACNNNNGTG	1 cut(s)	1346
Alw21I	GWGCWC	1 cut(s)	666
Alw26I	GTCTC	2 cut(s)	65, 728
AlwI	GGATC	2 cut(s)	1552, 1650
Ama87I	CYCGRG	2 cut(s)	757, 1525
Aor13HI	TCCGGA	1 cut(s)	457
Aor51HI	AGCGCT	1 cut(s)	651
AoxI	GGCC	6 cut(s)	182, 370, 563, 830, 1213, 1573
ApeKI	GCWGC	3 cut(s)	647, 1102, 1150
ApoI	RAATTY	3 cut(s)	104, 270, 986
ArsI	GACNNNNNNTTYG	2 cut(s)	959, 991
Asp700I	GAANNNNTTC	1 cut(s)	1609
AspA2I	CCTAGG	1 cut(s)	1433
AspLEI	GCGC	2 cut(s)	636, 652
AspS9I	GGNCC	7 cut(s)	182, 302, 440, 521, 563, 1129, 1573
AsuC2I	CCSGG	4 cut(s)	758, 759, 1526, 1527
AsuHPI	GGTGA	4 cut(s)	218, 352, 475, 1060
AsuII	TTCGAA	1 cut(s)	283
AsuNHI	GCTAGC	1 cut(s)	73
AvaI	CYCGRG	2 cut(s)	757, 1525
AvaII	GGWCC	4 cut(s)	302, 440, 521, 1129
AvrII	CCTAGG	1 cut(s)	1433
BaeGI	GKGCMC	2 cut(s)	383, 1485
BalI	TGGCCA	1 cut(s)	1215
BanI	GGYRCC	1 cut(s)	851
BanII	GRGCYC	2 cut(s)	666, 1285
Bbv12I	GWGCWC	1 cut(s)	666
BbvCI	CCTCAGC	1 cut(s)	69
BbvI	GCAGC	3 cut(s)	659, 1114, 1137
BccI	CCATC	6 cut(s)	263, 293, 440, 554, 1576, 1595
BcgI	CGANNNNNNTGC	2 cut(s)	254, 288
BciT130I	CCWGG	3 cut(s)	180, 561, 1126
BcnI	CCSGG	4 cut(s)	758, 759, 1526, 1527
BcoDI	GTCTC	2 cut(s)	65, 728
BcuI	ACTAGT	2 cut(s)	1343, 1370
BfaI	CTAG	8 cut(s)	27, 74, 198, 848, 1344, 1371, 1434, 1514
BfoI	RGCGCY	1 cut(s)	653
BglI	GCCNNNNNGGC	1 cut(s)	378
BisI	GCNGC	3 cut(s)	648, 1103, 1151
BlnI	CCTAGG	1 cut(s)	1433
BlsI	GCNGC	3 cut(s)	649, 1104, 1152
Bme1390I	CCNGG	7 cut(s)	180, 561, 758, 759, 1126, 1526, 1527
Bme18I	GGWCC	4 cut(s)	302, 440, 521, 1129
BmeT110I	CYCGRG	2 cut(s)	757, 1525
BmgT120I	GGNCC	7 cut(s)	182, 302, 440, 521, 563, 1129, 1573
BmiI	GGNNCC	7 cut(s)	442, 853, 1084, 1130, 1140, 1282, 1574
BmrFI	CCNGG	7 cut(s)	180, 561, 758, 759, 1126, 1526, 1527
BmrI	ACTGGG	4 cut(s)	297, 522, 950, 1015
BmsI	GCATC	2 cut(s)	526, 700
BmtI	GCTAGC	1 cut(s)	77
BmuI	ACTGGG	4 cut(s)	297, 522, 950, 1015
BplI	GAGNNNNNCTC	2 cut(s)	367, 399
BpmI	CTGGAG	1 cut(s)	1299
Bpu10I	CCTNAGC	1 cut(s)	69
Bpu14I	TTCGAA	1 cut(s)	283
BpuEI	CTTGAG	1 cut(s)	1574
BpuMI	CCSGG	4 cut(s)	758, 759, 1526, 1527
BsaAI	YACGTR	1 cut(s)	838
BsaI	GGTCTC	2 cut(s)	65, 728
BsaJI	CCNNGG	9 cut(s)	179, 559, 560, 756, 757, 1125, 1433, 1473, 1525
BsaWI	WCCGGW	1 cut(s)	457
BsaXI	ACNNNNNCTCC	6 cut(s)	921, 936, 951, 966, 1343, 1373
Bsc4I	CCNNNNNNNGG	7 cut(s)	261, 464, 697, 1490, 1491, 1494, 1582
Bse118I	RCCGGY	1 cut(s)	335
Bse1I	ACTGG	8 cut(s)	292, 517, 847, 947, 956, 1021, 1282, 1644
Bse3DI	GCAATG	4 cut(s)	675, 750, 1315, 1543
BseAI	TCCGGA	1 cut(s)	457
BseBI	CCWGG	3 cut(s)	180, 561, 1126
BseDI	CCNNGG	9 cut(s)	179, 559, 560, 756, 757, 1125, 1433, 1473, 1525
BseGI	GGATG	2 cut(s)	255, 1393
BseLI	CCNNNNNNNGG	7 cut(s)	261, 464, 697, 1490, 1491, 1494, 1582
BseMI	GCAATG	4 cut(s)	675, 750, 1315, 1543
BseMII	CTCAG	1 cut(s)	83
BseNI	ACTGG	8 cut(s)	292, 517, 847, 947, 956, 1021, 1282, 1644
BseRI	GAGGAG	1 cut(s)	206
BseSI	GKGCMC	2 cut(s)	383, 1485
BseXI	GCAGC	3 cut(s)	659, 1114, 1137
BseYI	CCCAGC	3 cut(s)	614, 1284, 1569
BshFI	GGCC	6 cut(s)	184, 372, 565, 832, 1215, 1575
BshNI	GGYRCC	1 cut(s)	851
BsiHKAI	GWGCWC	1 cut(s)	666
BsiHKCI	CYCGRG	2 cut(s)	757, 1525
BsiSI	CCGG	5 cut(s)	336, 458, 758, 1488, 1526
BslFI	GGGAC	3 cut(s)	426, 774, 1142
BslI	CCNNNNNNNGG	7 cut(s)	261, 464, 697, 1490, 1491, 1494, 1582
BsmAI	GTCTC	2 cut(s)	65, 728
BsmFI	GGGAC	3 cut(s)	426, 774, 1142
BsnI	GGCC	6 cut(s)	184, 372, 565, 832, 1215, 1575
Bso31I	GGTCTC	2 cut(s)	65, 728
BsoBI	CYCGRG	2 cut(s)	757, 1525
Bsp119I	TTCGAA	1 cut(s)	283
Bsp1286I	GDGCHC	4 cut(s)	383, 666, 1285, 1485
Bsp13I	TCCGGA	1 cut(s)	457
Bsp1407I	TGTACA	2 cut(s)	112, 351
Bsp143I	GATC	3 cut(s)	208, 1557, 1642
BspACI	CCGC	3 cut(s)	1188, 1491, 1634
BspANI	GGCC	6 cut(s)	184, 372, 565, 832, 1215, 1575
BspCNI	CTCAG	1 cut(s)	82
BspEI	TCCGGA	1 cut(s)	457
BspHI	TCATGA	1 cut(s)	819
BspLI	GGNNCC	7 cut(s)	442, 853, 1084, 1130, 1140, 1282, 1574
BspOI	GCTAGC	1 cut(s)	77
BspPI	GGATC	2 cut(s)	1552, 1650
BspQI	GCTCTTC	1 cut(s)	671
BspT104I	TTCGAA	1 cut(s)	283
BspT107I	GGYRCC	1 cut(s)	851
BspTNI	GGTCTC	2 cut(s)	65, 728
BsrDI	GCAATG	4 cut(s)	675, 750, 1315, 1543
BsrFI	RCCGGY	1 cut(s)	335
BsrGI	TGTACA	2 cut(s)	112, 351
BsrI	ACTGG	8 cut(s)	292, 517, 847, 947, 956, 1021, 1282, 1644
BssAI	RCCGGY	1 cut(s)	335
BssECI	CCNNGG	9 cut(s)	179, 559, 560, 756, 757, 1125, 1433, 1473, 1525
BssMI	GATC	3 cut(s)	208, 1557, 1642
BssT1I	CCWWGG	1 cut(s)	1433
Bst2UI	CCWGG	3 cut(s)	180, 561, 1126
Bst4CI	ACNGT	3 cut(s)	170, 1072, 1474
Bst6I	CTCTTC	2 cut(s)	671, 1404
BstAUI	TGTACA	2 cut(s)	112, 351
BstBAI	YACGTR	1 cut(s)	838
BstBI	TTCGAA	1 cut(s)	283
BstC8I	GCNNGC	5 cut(s)	75, 85, 396, 877, 1100
BstDEI	CTNAG	4 cut(s)	69, 413, 1317, 1597
BstDSI	CCRYGG	1 cut(s)	1473
BstF5I	GGATG	2 cut(s)	255, 1393
BstH2I	RGCGCY	1 cut(s)	653
BstHHI	GCGC	2 cut(s)	636, 652
BstKTI	GATC	3 cut(s)	211, 1560, 1645
BstMAI	GTCTC	2 cut(s)	65, 728
BstMBI	GATC	3 cut(s)	208, 1557, 1642
BstMWI	GCNNNNNNNGC	3 cut(s)	378, 1327, 1633
BstNI	CCWGG	3 cut(s)	180, 561, 1126
BstNSI	RCATGY	1 cut(s)	398
BstSCI	CCNGG	7 cut(s)	178, 559, 756, 757, 1124, 1524, 1525
BstSLI	GKGCMC	2 cut(s)	383, 1485
BstV1I	GCAGC	3 cut(s)	659, 1114, 1137
BstX2I	RGATCY	2 cut(s)	1557, 1642
BstXI	CCANNNNNNTGG	3 cut(s)	266, 1211, 1223
BstYI	RGATCY	2 cut(s)	1557, 1642
BsuRI	GGCC	6 cut(s)	184, 372, 565, 832, 1215, 1575
BtgI	CCRYGG	1 cut(s)	1473
BtsCI	GGATG	2 cut(s)	255, 1393
BtsIMutI	CAGTG	2 cut(s)	1275, 1637
Cac8I	GCNNGC	5 cut(s)	75, 85, 396, 877, 1100
CciI	TCATGA	1 cut(s)	819
CfoI	GCGC	2 cut(s)	636, 652
Cfr10I	RCCGGY	1 cut(s)	335
Cfr13I	GGNCC	7 cut(s)	182, 302, 440, 521, 563, 1129, 1573
Cfr9I	CCCGGG	2 cut(s)	757, 1525
CseI	GACGC	1 cut(s)	430
Csp6I	GTAC	3 cut(s)	113, 352, 1314
CviAII	CATG	9 cut(s)	236, 266, 395, 408, 820, 1092, 1143, 1409, 1540
CviQI	GTAC	3 cut(s)	113, 352, 1314
DdeI	CTNAG	4 cut(s)	69, 413, 1317, 1597
DpnI	GATC	3 cut(s)	210, 1559, 1644
DpnII	GATC	3 cut(s)	208, 1557, 1642
EaeI	YGGCCR	1 cut(s)	1213
Eam1104I	CTCTTC	2 cut(s)	671, 1404
EarI	CTCTTC	2 cut(s)	671, 1404
EciI	GGCGGA	1 cut(s)	1623
Ecl136II	GAGCTC	1 cut(s)	664
Eco130I	CCWWGG	1 cut(s)	1433
Eco24I	GRGCYC	2 cut(s)	666, 1285
Eco31I	GGTCTC	2 cut(s)	65, 728
Eco47I	GGWCC	4 cut(s)	302, 440, 521, 1129
Eco47III	AGCGCT	1 cut(s)	651
Eco53kI	GAGCTC	1 cut(s)	664
Eco57I	CTGAAG	2 cut(s)	655, 1598
Eco88I	CYCGRG	2 cut(s)	757, 1525
EcoICRI	GAGCTC	1 cut(s)	664
EcoO109I	RGGNCCY	1 cut(s)	1573
EcoRI	GAATTC	1 cut(s)	270
EcoRII	CCWGG	3 cut(s)	178, 559, 1124
EcoT14I	CCWWGG	1 cut(s)	1433
EcoT22I	ATGCAT	1 cut(s)	1545
EcoT38I	GRGCYC	2 cut(s)	666, 1285
ErhI	CCWWGG	1 cut(s)	1433
FaeI	CATG	9 cut(s)	239, 269, 398, 411, 823, 1095, 1146, 1412, 1543
FalI	AAGNNNNNCTT	2 cut(s)	488, 520
FaqI	GGGAC	3 cut(s)	426, 774, 1142
FatI	CATG	9 cut(s)	235, 265, 394, 407, 819, 1091, 1142, 1408, 1539
FauI	CCCGC	1 cut(s)	1484
Fnu4HI	GCNGC	3 cut(s)	648, 1103, 1151
FokI	GGATG	2 cut(s)	242, 1380
FriOI	GRGCYC	2 cut(s)	666, 1285
Fsp4HI	GCNGC	3 cut(s)	648, 1103, 1151
FspBI	CTAG	8 cut(s)	27, 74, 198, 848, 1344, 1371, 1434, 1514
GlaI	GCGC	2 cut(s)	635, 651
GluI	GCNGC	3 cut(s)	648, 1103, 1151
GsaI	CCCAGC	3 cut(s)	618, 1288, 1573
GsuI	CTGGAG	1 cut(s)	1299
HaeII	RGCGCY	1 cut(s)	653
HaeIII	GGCC	6 cut(s)	184, 372, 565, 832, 1215, 1575
HapII	CCGG	5 cut(s)	336, 458, 758, 1488, 1526
HgaI	GACGC	1 cut(s)	430
HhaI	GCGC	2 cut(s)	636, 652
Hin1II	CATG	9 cut(s)	239, 269, 398, 411, 823, 1095, 1146, 1412, 1543
Hin6I	GCGC	2 cut(s)	634, 650
HinP1I	GCGC	2 cut(s)	634, 650
HincII	GTYRAC	3 cut(s)	232, 1051, 1429
HindII	GTYRAC	3 cut(s)	232, 1051, 1429
HindIII	AAGCTT	1 cut(s)	1607
HinfI	GANTC	1 cut(s)	555
HpaI	GTTAAC	1 cut(s)	232
HpaII	CCGG	5 cut(s)	336, 458, 758, 1488, 1526
HphI	GGTGA	4 cut(s)	218, 352, 475, 1060
Hpy166II	GTNNAC	5 cut(s)	232, 352, 718, 1051, 1429
Hpy188I	TCNGA	3 cut(s)	242, 535, 1449
Hpy188III	TCNNGA	3 cut(s)	458, 733, 820
Hpy8I	GTNNAC	5 cut(s)	232, 352, 718, 1051, 1429
Hpy99I	CGWCG	1 cut(s)	662
HpyAV	CCTTC	2 cut(s)	638, 739
HpyCH4III	ACNGT	3 cut(s)	170, 1072, 1474
HpyCH4IV	ACGT	2 cut(s)	720, 837
HpyF10VI	GCNNNNNNNGC	3 cut(s)	378, 1327, 1633
HpyF3I	CTNAG	4 cut(s)	69, 413, 1317, 1597
HpySE526I	ACGT	2 cut(s)	720, 837
Hsp92II	CATG	9 cut(s)	239, 269, 398, 411, 823, 1095, 1146, 1412, 1543
HspAI	GCGC	2 cut(s)	634, 650
Kpn2I	TCCGGA	1 cut(s)	457
KspAI	GTTAAC	1 cut(s)	232
Kzo9I	GATC	3 cut(s)	208, 1557, 1642
LguI	GCTCTTC	1 cut(s)	671
LmnI	GCTCC	3 cut(s)	82, 460, 1280
Lsp1109I	GCAGC	3 cut(s)	659, 1114, 1137
LweI	GCATC	2 cut(s)	526, 700
MaeI	CTAG	8 cut(s)	27, 74, 198, 848, 1344, 1371, 1434, 1514
MaeII	ACGT	2 cut(s)	720, 837
MaeIII	GTNAC	3 cut(s)	127, 157, 838
MalI	GATC	3 cut(s)	210, 1559, 1644
MboI	GATC	3 cut(s)	208, 1557, 1642
MboII	GAAGA	8 cut(s)	446, 658, 661, 770, 1057, 1391, 1397, 1591
MflI	RGATCY	2 cut(s)	1557, 1642
MhlI	GDGCHC	4 cut(s)	383, 666, 1285, 1485
MlsI	TGGCCA	1 cut(s)	1215
MluCI	AATT	9 cut(s)	104, 173, 189, 270, 986, 1240, 1260, 1270, 1417
MluNI	TGGCCA	1 cut(s)	1215
MlyI	GAGTC	1 cut(s)	549
Mox20I	TGGCCA	1 cut(s)	1215
Mph1103I	ATGCAT	1 cut(s)	1545
MroI	TCCGGA	1 cut(s)	457
MroXI	GAANNNNTTC	1 cut(s)	1609
MscI	TGGCCA	1 cut(s)	1215
MseI	TTAA	6 cut(s)	222, 231, 1259, 1269, 1338, 1416
MslI	CAYNNNNRTG	3 cut(s)	264, 1346, 1407
Msp20I	TGGCCA	1 cut(s)	1215
MspA1I	CMGCKG	2 cut(s)	1288, 1569
MspI	CCGG	5 cut(s)	336, 458, 758, 1488, 1526
MspR9I	CCNGG	7 cut(s)	180, 561, 758, 759, 1126, 1526, 1527
MvaI	CCWGG	3 cut(s)	180, 561, 1126
MwoI	GCNNNNNNNGC	3 cut(s)	378, 1327, 1633
NciI	CCSGG	4 cut(s)	758, 759, 1526, 1527
NdeII	GATC	3 cut(s)	208, 1557, 1642
NheI	GCTAGC	1 cut(s)	73
NlaIII	CATG	9 cut(s)	239, 269, 398, 411, 823, 1095, 1146, 1412, 1543
NlaIV	GGNNCC	7 cut(s)	442, 853, 1084, 1130, 1140, 1282, 1574
NmuCI	GTSAC	2 cut(s)	127, 838
NsiI	ATGCAT	1 cut(s)	1545
NspI	RCATGY	1 cut(s)	398
NspV	TTCGAA	1 cut(s)	283
OliI	CACNNNNGTG	1 cut(s)	1346
PaeI	GCATGC	1 cut(s)	398
PagI	TCATGA	1 cut(s)	819
PasI	CCCWGGG	1 cut(s)	560
PciSI	GCTCTTC	1 cut(s)	671
PcsI	WCGNNNNNNNCGW	1 cut(s)	280
PdmI	GAANNNNTTC	1 cut(s)	1609
PflMI	CCANNNNNTGG	1 cut(s)	261
PkrI	GCNGC	3 cut(s)	649, 1104, 1152
PleI	GAGTC	1 cut(s)	549
PpsI	GAGTC	1 cut(s)	549
Ppu21I	YACGTR	1 cut(s)	838
Psp124BI	GAGCTC	1 cut(s)	666
Psp6I	CCWGG	3 cut(s)	178, 559, 1124
PspFI	CCCAGC	3 cut(s)	614, 1284, 1569
PspGI	CCWGG	3 cut(s)	178, 559, 1124
PspN4I	GGNNCC	7 cut(s)	442, 853, 1084, 1130, 1140, 1282, 1574
PspPI	GGNCC	7 cut(s)	182, 302, 440, 521, 563, 1129, 1573
PsuI	RGATCY	2 cut(s)	1557, 1642
PvuII	CAGCTG	2 cut(s)	1288, 1569
RsaI	GTAC	3 cut(s)	114, 353, 1315
RsaNI	GTAC	3 cut(s)	113, 352, 1314
RseI	CAYNNNNRTG	3 cut(s)	264, 1346, 1407
SacI	GAGCTC	1 cut(s)	666
SapI	GCTCTTC	1 cut(s)	671
SaqAI	TTAA	6 cut(s)	222, 231, 1259, 1269, 1338, 1416
SatI	GCNGC	3 cut(s)	648, 1103, 1151
Sau3AI	GATC	3 cut(s)	208, 1557, 1642
Sau96I	GGNCC	7 cut(s)	182, 302, 440, 521, 563, 1129, 1573
SchI	GAGTC	1 cut(s)	549
ScrFI	CCNGG	7 cut(s)	180, 561, 758, 759, 1126, 1526, 1527
SduI	GDGCHC	4 cut(s)	383, 666, 1285, 1485
SfaNI	GCATC	2 cut(s)	526, 700
SfuI	TTCGAA	1 cut(s)	283
SinI	GGWCC	4 cut(s)	302, 440, 521, 1129
SmaI	CCCGGG	2 cut(s)	759, 1527
SmiMI	CAYNNNNRTG	3 cut(s)	264, 1346, 1407
SmlI	CTYRAG	1 cut(s)	1553
SmoI	CTYRAG	1 cut(s)	1553
SpeI	ACTAGT	2 cut(s)	1343, 1370
SphI	GCATGC	1 cut(s)	398
Sse9I	AATT	9 cut(s)	104, 173, 189, 270, 986, 1240, 1260, 1270, 1417
SsiI	CCGC	3 cut(s)	1188, 1491, 1634
SspMI	CTAG	8 cut(s)	27, 74, 198, 848, 1344, 1371, 1434, 1514
SstI	GAGCTC	1 cut(s)	666
StyD4I	CCNGG	7 cut(s)	178, 559, 756, 757, 1124, 1524, 1525
StyI	CCWWGG	1 cut(s)	1433
TaaI	ACNGT	3 cut(s)	170, 1072, 1474
TaiI	ACGT	2 cut(s)	723, 840
TaqI	TCGA	4 cut(s)	187, 274, 283, 732
TaqII	GACCGA	1 cut(s)	1146
TasI	AATT	9 cut(s)	104, 173, 189, 270, 986, 1240, 1260, 1270, 1417
TatI	WGTACW	2 cut(s)	112, 351
Tru1I	TTAA	6 cut(s)	222, 231, 1259, 1269, 1338, 1416
Tru9I	TTAA	6 cut(s)	222, 231, 1259, 1269, 1338, 1416
TscAI	CASTG	2 cut(s)	1282, 1644
TseFI	GTSAC	2 cut(s)	127, 838
TseI	GCWGC	3 cut(s)	647, 1102, 1150
Tsp45I	GTSAC	2 cut(s)	127, 838
TspDTI	ATGAA	9 cut(s)	9, 37, 396, 808, 896, 938, 1391, 1397, 1528
TspGWI	ACGGA	1 cut(s)	992
TspMI	CCCGGG	2 cut(s)	757, 1525
TspRI	CASTG	2 cut(s)	1282, 1644
Van91I	CCANNNNNTGG	1 cut(s)	261
VpaK11BI	GGWCC	4 cut(s)	302, 440, 521, 1129
XapI	RAATTY	3 cut(s)	104, 270, 986
XceI	RCATGY	1 cut(s)	398
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1132
XmaI	CCCGGG	2 cut(s)	757, 1525
XmaJI	CCTAGG	1 cut(s)	1433
XmnI	GAANNNNTTC	1 cut(s)	1609
XspI	CTAG	8 cut(s)	27, 74, 198, 848, 1344, 1371, 1434, 1514
Zsp2I	ATGCAT	1 cut(s)	1545

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.