MD12G1144600.v1.1

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000610
Physical Location & Seq
Reverse (-)
22375740 .. 22378559
2820 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1144600.v1.1.491

Sequence Viewer

Length: 1836 bp
ATGCCTGATTATCTTAATCTTTCTTGCATCCTTACAACTCAAAGTCATCAGCATTTGAAAAAGAATTCAGCAGATCGAGTGATCAGTGACATTAGAGAGATGGGTTCAAGTTCATTAGCAATCTTCTTTGCAGTTTGTGCATTTATATGTGTGTTTTCTGAGCCTGCACTTGCAAAGCATGCTAGGGTTACCAGGCATTACAACTTTAATATAAAATCACAAAGTGTCACAAGGCTTTGTCAAACCAAGAGCACTGTGACTGTCAACGGGCAGTTTCCCGGGCCTCGGATCATCGCAAGGGAAGGGGACAGGCTTGTGATCAAGGTGGTGAATAATGTCCAGCACAATGTCACACTCCATTGGCATGGGATCAGGCAATTGAGGACTGGATGGGCAGATGGACCTGCATATATCACACAGTGCCCAATTCAGACCGGGCAAAGTTATGTCTACAACTTCACTATTACAGGCCAAAGAGGCACATTGTTTTGGCATGCCCACATCTCCTGGCTTAGGGCAACTGTCTATGGACCTATAGTCATCCTCCCTAAAAGACATGTGCCTTACCCTTTCCCCCAACCCTTCCAAGAAGTTCCCATCATTTTTGGTGAATGGTGGAAAGCAGACACAGAAAAAGTAATCAACCAAGCCTTACAAACAGGGTTGGGACCAAATGTCTCTGATGCCTATACCATCAATGGTCTTCCAGGGCCTTTGTACAACTGCTCAACCAAAGATATATACAAGCTCAAGGTGAAGCCGGGGAGGACGTATCTGCTGCGGTTGATCAACGCTGCGCTCAACAACGAGCTTTTCTTCAGCATTGCCAACCATACCCTAACTGTAGTCGAAGCAGATGCAGTTTATGTGAAGCCATTCAAAACCCAAACTGTTCTGATCACACCAGGACAGACCACAAATGTGCTTCTAATGACAAAACCTAAGTCCCCGAATGCAAAATTTGTCATGGCAGCTCGCCCATATGTGTCCGGCCCAGCTGCTTTCGACAACTCTACAACCACAGGATTACTAGAATATGAAATTTCTTCTTCCTCAAGCAACAGCAAGAACAATAAAAAGCAGCTTTTCACACCAGTGCTTCCAAAGTTCAATGATACATTATTTTCCATGAAATACAACAAGAAAATTCTCAGCTTGGCTAGTCCCAAATTCCCGGCGGCAAATGTCCCAAAAACTGTGGAAAAGCGCTTCTTCTTCACTGTTGGGTTGGGAATACTCCCATGCTTAAAAAAACAAACATGCCAAGGCCCAAATAATACTAGGTTAGCAGCGGCCATTAACAATGTGACATTTGTCCAACCCAATACAGCTCTTCTGCAAGCTCATTTCTTCAACCAATCAAAGGGTGTGTACACCACAGATTTCCCAGCAAACCCAGCAGTCAAATTCAACTACACAGGGAGTCCACCCAACAACATTGCCGTGAGCAGTGGGACGAAGGTGGTGGTGCTGCCTTTCAACACTAGTGTGGAGTTGGTTTTGCAGGACACTAGCATTATTGGGGCTGAGAGCCACCCGCTTCACCTACATGGGTTTAATTTCTTTGTGCTGGGATTGGGCTCTGGAAACTTTGATAAAAAAAATGACCCTAAAAAGTATAACCTGGTTGATCCTGCAGAGAGGAACACCATCGGTGTCCCCTCTGGTGGCTGGGTTGCCATTCGGTTTCTCGCCGATAATCCAGGTGTTTGGTTCATGCATTGCCACCTAGAAGTGCACACCAGCTGGGGGTTGAAGATGGCTTGGGTGGTCTTGGATGGTAAGAGACGCAACCAAAAGCTACCTCCTCCACCGTCCGATCTTCCCAAGTGTTAA

Protein Analysis

612

Amino Acids

67.87

Weight (kDa)

9.7

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 71 - 184 6.4e-43 Multicopper oxidase
Cu-oxidase PF00394 197 - 347 2.4e-42 Multicopper oxidase
Cu-oxidase_2 PF07731 462 - 593 1.3e-41 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000610)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60020 AT5G60020 AT5G60020
fragaria_vesca FvH4_2g09062 FvH4_2g09062 FvH4_3g05920 FvH4_3g34100 FvH4_3g34310 FvH4_3g34340 FvH4_6g13390
malus_domestica MD00G1015000.v1.1 MD03G1106500.v1.1 MD03G1106600.v1.1 MD04G1131500.v1.1 MD11G1118900.v1.1 MD11G1119000.v1.1 MD12G1144600.v1.1
prunus_persica Prupe.6G089100_v2.0.a1 Prupe.6G089300_v2.0.a1 Prupe.6G258000_v2.0.a1
pyrus_communis pycom03g08590 pycom10g02940 pycom11g09990 pycom11g10060
rosa_chinensis RchiOBHm_Chr2g0154491 RchiOBHm_Chr3g0466271 RchiOBHm_Chr5g0061891 RchiOBHm_Chr5g0061911 RchiOBHm_Chr5g0061951 RchiOBHm_Chr5g0061961 RchiOBHm_Chr6g0264371
rosa_laevigata RLG00000024591 RLG00000035514 RLG00000035516 RLG00000035518 RLG00000035519
rosa_multiflora Rmu_sc0003480.1_g000021 Rmu_sc0011641.1_g000002 Rmu_sc0011641.1_g000009
rosa_roxburghii Rroxscaffold_1G00018790 Rroxscaffold_1G00018820 Rroxscaffold_1G00018850 Rroxscaffold_2G00122520 Rroxscaffold_2G00122540 Rroxscaffold_6G00414660
rosa_rugosa Rorug03G0080700 Rorug04G0180900 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345100 Rorug05G0345200 Rorug06G0011800
rosa_samantha Rh3DG148900 Rh5AG405700 Rh5AG406100 Rh5AG406200 Rh5BG419200 Rh5BG419400 Rh5CG443100 Rh5CG443300 Rh5CG443500 Rh5CG443600 Rh5DG432700 Rh5DG434900 Rh6BG128700 Rh6CG127600 Rh6DG114200 Rh6DG114300
rosa_wichuraiana Rw3G011980 Rw5G038100 Rw5G038140 Rw5G038160 Rw5G038200 Rw5G038210 Rw5G038250 Rw5G038270 Rw5G038290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 412
AccI GTMKAC 1 cut(s) 450
AciI CCGC 4 cut(s) 781, 1178, 1292, 1538
AclWI GGATC 3 cut(s) 296, 377, 1625
AcoI YGGCCR 1 cut(s) 1293
AcsI RAATTY 6 cut(s) 64, 959, 1041, 1146, 1169, 1406
AcuI CTGAAG 1 cut(s) 802
AdeI CACNNNGTG 2 cut(s) 224, 420
AfaI GTAC 2 cut(s) 719, 1373
AfeI AGCGCT 1 cut(s) 1208
AfiI CCNNNNNNNGG 5 cut(s) 285, 513, 1363, 1667, 1749
AflIII ACRYGT 1 cut(s) 556
AgsI TTSAA 8 cut(s) 58, 108, 880, 1111, 1354, 1411, 1480, 1756
AhdI GACNNNNNGTC 2 cut(s) 536, 674
AhlI ACTAGT 1 cut(s) 1484
AjnI CCWGG 6 cut(s) 191, 506, 706, 904, 1623, 1702
AleI CACNNNNGTG 3 cut(s) 920, 1094, 1487
Alw21I GWGCWC 2 cut(s) 254, 1740
Alw26I GTCTC 2 cut(s) 682, 1780
Alw44I GTGCAC 1 cut(s) 1736
AlwI GGATC 3 cut(s) 296, 377, 1625
Ama87I CYCGRG 1 cut(s) 278
Aor51HI AGCGCT 1 cut(s) 1208
AoxI GGCC 6 cut(s) 281, 469, 710, 991, 1267, 1293
ApaLI GTGCAC 1 cut(s) 1736
ApeKI GCWGC 7 cut(s) 778, 794, 971, 998, 1081, 1289, 1471
ApoI RAATTY 6 cut(s) 64, 959, 1041, 1146, 1169, 1406
ArsI GACNNNNNNTTYG 2 cut(s) 929, 961
Asp700I GAANNNNTTC 1 cut(s) 875
AspLEI GCGC 2 cut(s) 799, 1209
AspS9I GGNCC 7 cut(s) 281, 401, 530, 668, 710, 992, 1268
AsuC2I CCSGG 5 cut(s) 279, 280, 436, 762, 1175
AsuHPI GGTGA 4 cut(s) 340, 620, 766, 1535
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 3 cut(s) 401, 530, 668
BaeGI GKGCMC 2 cut(s) 425, 1740
BanII GRGCYC 1 cut(s) 1583
BbsI GAAGAC 1 cut(s) 695
Bbv12I GWGCWC 2 cut(s) 254, 1740
BbvI GCAGC 7 cut(s) 765, 781, 983, 985, 1093, 1301, 1458
BccI CCATC 8 cut(s) 94, 384, 392, 605, 701, 1658, 1753, 1772
BceAI ACGGC 1 cut(s) 1427
BcgI CGANNNNNNTGC 2 cut(s) 839, 873
BciT130I CCWGG 6 cut(s) 193, 508, 708, 906, 1625, 1704
BclI TGATCA 4 cut(s) 81, 318, 786, 897
BcnI CCSGG 5 cut(s) 279, 280, 436, 762, 1175
BcoDI GTCTC 2 cut(s) 682, 1780
BcuI ACTAGT 1 cut(s) 1484
BfaI CTAG 7 cut(s) 183, 1031, 1161, 1281, 1485, 1512, 1730
BfmI CTRYAG 3 cut(s) 534, 843, 1635
BfoI RGCGCY 1 cut(s) 1210
BfuAI ACCTGC 1 cut(s) 412
BglI GCCNNNNNGGC 1 cut(s) 477
BisI GCNGC 9 cut(s) 779, 795, 972, 999, 1082, 1179, 1290, 1293, 1472
BlsI GCNGC 9 cut(s) 780, 796, 973, 1000, 1083, 1180, 1291, 1294, 1473
Bme18I GGWCC 3 cut(s) 401, 530, 668
BmeRI GACNNNNNGTC 2 cut(s) 536, 674
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 7 cut(s) 281, 401, 530, 668, 710, 992, 1268
BmiI GGNNCC 1 cut(s) 669
BmsI GCATC 3 cut(s) 36, 673, 847
BoxI GACNNNNGTC 1 cut(s) 1313
BpiI GAAGAC 1 cut(s) 695
Bpu10I CCTNAGC 1 cut(s) 512
BpuEI CTTGAG 2 cut(s) 734, 1039
BpuMI CCSGG 5 cut(s) 279, 280, 436, 762, 1175
BsaJI CCNNGG 5 cut(s) 278, 284, 707, 761, 1264
Bsc4I CCNNNNNNNGG 5 cut(s) 285, 513, 1363, 1667, 1749
Bse1I ACTGG 2 cut(s) 391, 1094
Bse3DI GCAATG 3 cut(s) 822, 1437, 1720
BseBI CCWGG 6 cut(s) 193, 508, 708, 906, 1625, 1704
BseDI CCNNGG 5 cut(s) 278, 284, 707, 761, 1264
BseGI GGATG 4 cut(s) 27, 395, 540, 1783
BseLI CCNNNNNNNGG 5 cut(s) 285, 513, 1363, 1667, 1749
BseMI GCAATG 3 cut(s) 822, 1437, 1720
BseMII CTCAG 3 cut(s) 150, 1165, 1518
BseNI ACTGG 2 cut(s) 391, 1094
BseRI GAGGAG 1 cut(s) 1797
BseSI GKGCMC 2 cut(s) 425, 1740
BseXI GCAGC 7 cut(s) 765, 781, 983, 985, 1093, 1301, 1458
BseYI CCCAGC 6 cut(s) 994, 1387, 1396, 1570, 1671, 1746
BsgI GTGCAG 1 cut(s) 150
BshFI GGCC 6 cut(s) 283, 471, 712, 993, 1269, 1295
BsiHKAI GWGCWC 2 cut(s) 254, 1740
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 5 cut(s) 279, 435, 761, 990, 1175
BslFI GGGAC 7 cut(s) 320, 681, 931, 1149, 1172, 1468, 1643
BslI CCNNNNNNNGG 5 cut(s) 285, 513, 1363, 1667, 1749
BsmAI GTCTC 2 cut(s) 682, 1780
BsmBI CGTCTC 1 cut(s) 1780
BsmFI GGGAC 7 cut(s) 320, 681, 931, 1149, 1172, 1468, 1643
BsmI GAATGC 1 cut(s) 958
BsnI GGCC 6 cut(s) 283, 471, 712, 993, 1269, 1295
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 4 cut(s) 254, 425, 1583, 1740
Bsp1407I TGTACA 2 cut(s) 717, 1371
Bsp143I GATC 9 cut(s) 73, 81, 288, 318, 369, 786, 897, 1630, 1819
BspACI CCGC 4 cut(s) 781, 1178, 1292, 1538
BspANI GGCC 6 cut(s) 283, 471, 712, 993, 1269, 1295
BspCNI CTCAG 3 cut(s) 151, 1164, 1519
BspLI GGNNCC 1 cut(s) 669
BspMAI CTGCAG 1 cut(s) 1639
BspMI ACCTGC 1 cut(s) 412
BspPI GGATC 3 cut(s) 296, 377, 1625
BspQI GCTCTTC 1 cut(s) 1338
BsrDI GCAATG 3 cut(s) 822, 1437, 1720
BsrGI TGTACA 2 cut(s) 717, 1371
BsrI ACTGG 2 cut(s) 391, 1094
BssECI CCNNGG 5 cut(s) 278, 284, 707, 761, 1264
BssMI GATC 9 cut(s) 73, 81, 288, 318, 369, 786, 897, 1630, 1819
BssT1I CCWWGG 1 cut(s) 1264
Bst2UI CCWGG 6 cut(s) 193, 508, 708, 906, 1625, 1704
Bst4CI ACNGT 9 cut(s) 256, 262, 420, 523, 844, 892, 1198, 1222, 1815
Bst6I CTCTTC 1 cut(s) 1338
BstAPI GCANNNNNTGC 2 cut(s) 137, 179
BstAUI TGTACA 2 cut(s) 717, 1371
BstC8I GCNNGC 5 cut(s) 165, 180, 495, 976, 1341
BstDEI CTNAG 5 cut(s) 159, 512, 942, 1151, 1527
BstEII GGTNACC 1 cut(s) 187
BstENI CCTNNNNNAGG 1 cut(s) 511
BstF5I GGATG 4 cut(s) 27, 395, 540, 1783
BstH2I RGCGCY 1 cut(s) 1210
BstHHI GCGC 2 cut(s) 799, 1209
BstKTI GATC 9 cut(s) 76, 84, 291, 321, 372, 789, 900, 1633, 1822
BstMAI GTCTC 2 cut(s) 682, 1780
BstMBI GATC 9 cut(s) 73, 81, 288, 318, 369, 786, 897, 1630, 1819
BstMWI GCNNNNNNNGC 4 cut(s) 137, 179, 477, 1397
BstNI CCWGG 6 cut(s) 193, 508, 708, 906, 1625, 1704
BstNSI RCATGY 4 cut(s) 182, 497, 560, 1263
BstPAI GACNNNNGTC 1 cut(s) 1313
BstPI GGTNACC 1 cut(s) 187
BstSFI CTRYAG 3 cut(s) 534, 843, 1635
BstSLI GKGCMC 2 cut(s) 425, 1740
BstV1I GCAGC 7 cut(s) 765, 781, 983, 985, 1093, 1301, 1458
BstV2I GAAGAC 1 cut(s) 695
BstXI CCANNNNNNTGG 2 cut(s) 365, 1710
BsuRI GGCC 6 cut(s) 283, 471, 712, 993, 1269, 1295
BtgZI GCGATG 1 cut(s) 277
BtsCI GGATG 4 cut(s) 27, 395, 540, 1783
BtsI GCAGTG 1 cut(s) 1456
BtsIMutI CAGTG 6 cut(s) 91, 252, 425, 1101, 1218, 1456
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 5 cut(s) 165, 180, 495, 976, 1341
CfoI GCGC 2 cut(s) 799, 1209
Cfr13I GGNCC 7 cut(s) 281, 401, 530, 668, 710, 992, 1268
Cfr9I CCCGGG 1 cut(s) 278
CseI GACGC 1 cut(s) 1797
CsiI ACCWGGT 1 cut(s) 1623
Csp6I GTAC 2 cut(s) 718, 1372
CspCI CAANNNNNGTGG 2 cut(s) 1179, 1214
CviQI GTAC 2 cut(s) 718, 1372
DdeI CTNAG 5 cut(s) 159, 512, 942, 1151, 1527
DpnI GATC 9 cut(s) 75, 83, 290, 320, 371, 788, 899, 1632, 1821
DpnII GATC 9 cut(s) 73, 81, 288, 318, 369, 786, 897, 1630, 1819
DraIII CACNNNGTG 2 cut(s) 224, 420
DriI GACNNNNNGTC 2 cut(s) 536, 674
EaeI YGGCCR 1 cut(s) 1293
Eam1104I CTCTTC 1 cut(s) 1338
Eam1105I GACNNNNNGTC 2 cut(s) 536, 674
EarI CTCTTC 1 cut(s) 1338
Eco130I CCWWGG 1 cut(s) 1264
Eco24I GRGCYC 1 cut(s) 1583
Eco47I GGWCC 3 cut(s) 401, 530, 668
Eco47III AGCGCT 1 cut(s) 1208
Eco57I CTGAAG 1 cut(s) 802
Eco88I CYCGRG 1 cut(s) 278
Eco91I GGTNACC 1 cut(s) 187
EcoNI CCTNNNNNAGG 1 cut(s) 511
EcoO109I RGGNCCY 1 cut(s) 710
EcoO65I GGTNACC 1 cut(s) 187
EcoRI GAATTC 1 cut(s) 64
EcoRII CCWGG 6 cut(s) 191, 506, 706, 904, 1623, 1702
EcoT14I CCWWGG 1 cut(s) 1264
EcoT22I ATGCAT 1 cut(s) 1722
EcoT38I GRGCYC 1 cut(s) 1583
ErhI CCWWGG 1 cut(s) 1264
Esp3I CGTCTC 1 cut(s) 1780
FalI AAGNNNNNCTT 2 cut(s) 1196, 1228
FaqI GGGAC 7 cut(s) 320, 681, 931, 1149, 1172, 1468, 1643
FauI CCCGC 1 cut(s) 1545
FauNDI CATATG 1 cut(s) 982
FbaI TGATCA 4 cut(s) 81, 318, 786, 897
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 9 cut(s) 779, 795, 972, 999, 1082, 1179, 1290, 1293, 1472
FokI GGATG 4 cut(s) 14, 402, 527, 1790
FriOI GRGCYC 1 cut(s) 1583
Fsp4HI GCNGC 9 cut(s) 779, 795, 972, 999, 1082, 1179, 1290, 1293, 1472
FspBI CTAG 7 cut(s) 183, 1031, 1161, 1281, 1485, 1512, 1730
GlaI GCGC 2 cut(s) 798, 1208
GluI GCNGC 9 cut(s) 779, 795, 972, 999, 1082, 1179, 1290, 1293, 1472
GsaI CCCAGC 6 cut(s) 998, 1391, 1400, 1574, 1675, 1750
HaeII RGCGCY 1 cut(s) 1210
HaeIII GGCC 6 cut(s) 283, 471, 712, 993, 1269, 1295
HapII CCGG 5 cut(s) 279, 435, 761, 990, 1175
HgaI GACGC 1 cut(s) 1797
HhaI GCGC 2 cut(s) 799, 1209
Hin6I GCGC 2 cut(s) 797, 1207
HinP1I GCGC 2 cut(s) 797, 1207
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 1 cut(s) 1423
HpaII CCGG 5 cut(s) 279, 435, 761, 990, 1175
HphI GGTGA 4 cut(s) 340, 620, 766, 1535
Hpy166II GTNNAC 6 cut(s) 265, 451, 1372, 1374, 1427, 1738
Hpy188I TCNGA 6 cut(s) 160, 288, 432, 682, 897, 1819
Hpy188III TCNNGA 1 cut(s) 1584
Hpy8I GTNNAC 6 cut(s) 265, 451, 1372, 1374, 1427, 1738
HpyAV CCTTC 3 cut(s) 296, 592, 1453
HpyCH4III ACNGT 9 cut(s) 256, 262, 420, 523, 844, 892, 1198, 1222, 1815
HpyCH4IV ACGT 1 cut(s) 770
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 179, 477, 1397
HpyF3I CTNAG 5 cut(s) 159, 512, 942, 1151, 1527
HpySE526I ACGT 1 cut(s) 770
HspAI GCGC 2 cut(s) 797, 1207
Ksp22I TGATCA 4 cut(s) 81, 318, 786, 897
Kzo9I GATC 9 cut(s) 73, 81, 288, 318, 369, 786, 897, 1630, 1819
LguI GCTCTTC 1 cut(s) 1338
Lsp1109I GCAGC 7 cut(s) 765, 781, 983, 985, 1093, 1301, 1458
LweI GCATC 3 cut(s) 36, 673, 847
MabI ACCWGGT 1 cut(s) 1623
MaeI CTAG 7 cut(s) 183, 1031, 1161, 1281, 1485, 1512, 1730
MaeII ACGT 1 cut(s) 770
MaeIII GTNAC 6 cut(s) 86, 187, 226, 256, 349, 1306
MalI GATC 9 cut(s) 75, 83, 290, 320, 371, 788, 899, 1632, 1821
MboI GATC 9 cut(s) 73, 81, 288, 318, 369, 786, 897, 1630, 1819
MfeI CAATTG 1 cut(s) 377
MhlI GDGCHC 4 cut(s) 254, 425, 1583, 1740
MluCI AATT 9 cut(s) 64, 377, 426, 959, 1041, 1146, 1169, 1406, 1558
MlyI GAGTC 1 cut(s) 1432
MmeI TCCRAC 1 cut(s) 1342
Mph1103I ATGCAT 1 cut(s) 1722
MroXI GAANNNNTTC 1 cut(s) 875
MseI TTAA 6 cut(s) 15, 207, 1247, 1299, 1557, 1834
MslI CAYNNNNRTG 7 cut(s) 145, 363, 920, 1094, 1442, 1487, 1548
MspA1I CMGCKG 3 cut(s) 998, 1292, 1746
MspI CCGG 5 cut(s) 279, 435, 761, 990, 1175
MunI CAATTG 1 cut(s) 377
Mva1269I GAATGC 1 cut(s) 958
MvaI CCWGG 6 cut(s) 193, 508, 708, 906, 1625, 1704
MwoI GCNNNNNNNGC 4 cut(s) 137, 179, 477, 1397
NciI CCSGG 5 cut(s) 279, 280, 436, 762, 1175
NdeI CATATG 1 cut(s) 982
NdeII GATC 9 cut(s) 73, 81, 288, 318, 369, 786, 897, 1630, 1819
NlaIV GGNNCC 1 cut(s) 669
NmuCI GTSAC 5 cut(s) 86, 226, 256, 349, 1306
NsiI ATGCAT 1 cut(s) 1722
NspI RCATGY 4 cut(s) 182, 497, 560, 1263
OliI CACNNNNGTG 3 cut(s) 920, 1094, 1487
PaeI GCATGC 2 cut(s) 182, 497
PciI ACATGT 1 cut(s) 556
PciSI GCTCTTC 1 cut(s) 1338
PctI GAATGC 1 cut(s) 958
PdmI GAANNNNTTC 1 cut(s) 875
PkrI GCNGC 9 cut(s) 780, 796, 973, 1000, 1083, 1180, 1291, 1294, 1473
PleI GAGTC 1 cut(s) 1431
PpsI GAGTC 1 cut(s) 1431
PscI ACATGT 1 cut(s) 556
PshAI GACNNNNGTC 1 cut(s) 1313
Psp6I CCWGG 6 cut(s) 191, 506, 706, 904, 1623, 1702
PspEI GGTNACC 1 cut(s) 187
PspFI CCCAGC 6 cut(s) 994, 1387, 1396, 1570, 1671, 1746
PspGI CCWGG 6 cut(s) 191, 506, 706, 904, 1623, 1702
PspN4I GGNNCC 1 cut(s) 669
PspPI GGNCC 7 cut(s) 281, 401, 530, 668, 710, 992, 1268
PstI CTGCAG 1 cut(s) 1639
PvuII CAGCTG 2 cut(s) 998, 1746
RsaI GTAC 2 cut(s) 719, 1373
RsaNI GTAC 2 cut(s) 718, 1372
RseI CAYNNNNRTG 7 cut(s) 145, 363, 920, 1094, 1442, 1487, 1548
SapI GCTCTTC 1 cut(s) 1338
SaqAI TTAA 6 cut(s) 15, 207, 1247, 1299, 1557, 1834
SatI GCNGC 9 cut(s) 779, 795, 972, 999, 1082, 1179, 1290, 1293, 1472
Sau3AI GATC 9 cut(s) 73, 81, 288, 318, 369, 786, 897, 1630, 1819
Sau96I GGNCC 7 cut(s) 281, 401, 530, 668, 710, 992, 1268
SchI GAGTC 1 cut(s) 1432
SduI GDGCHC 4 cut(s) 254, 425, 1583, 1740
SexAI ACCWGGT 1 cut(s) 1623
SfaNI GCATC 3 cut(s) 36, 673, 847
SfcI CTRYAG 3 cut(s) 534, 843, 1635
SinI GGWCC 3 cut(s) 401, 530, 668
SmaI CCCGGG 1 cut(s) 280
SmiMI CAYNNNNRTG 7 cut(s) 145, 363, 920, 1094, 1442, 1487, 1548
SmlI CTYRAG 2 cut(s) 749, 1054
SmoI CTYRAG 2 cut(s) 749, 1054
SpeI ACTAGT 1 cut(s) 1484
SphI GCATGC 2 cut(s) 182, 497
Sse9I AATT 9 cut(s) 64, 377, 426, 959, 1041, 1146, 1169, 1406, 1558
SsiI CCGC 4 cut(s) 781, 1178, 1292, 1538
SspMI CTAG 7 cut(s) 183, 1031, 1161, 1281, 1485, 1512, 1730
StyI CCWWGG 1 cut(s) 1264
TaaI ACNGT 9 cut(s) 256, 262, 420, 523, 844, 892, 1198, 1222, 1815
TaiI ACGT 1 cut(s) 773
TaqI TCGA 3 cut(s) 76, 849, 1005
TasI AATT 9 cut(s) 64, 377, 426, 959, 1041, 1146, 1169, 1406, 1558
TatI WGTACW 2 cut(s) 717, 1371
TauI GCSGC 2 cut(s) 1181, 1295
Tru1I TTAA 6 cut(s) 15, 207, 1247, 1299, 1557, 1834
Tru9I TTAA 6 cut(s) 15, 207, 1247, 1299, 1557, 1834
TscAI CASTG 6 cut(s) 91, 259, 425, 1101, 1225, 1456
TseFI GTSAC 5 cut(s) 86, 226, 256, 349, 1306
TseI GCWGC 7 cut(s) 778, 794, 971, 998, 1081, 1289, 1471
Tsp45I GTSAC 5 cut(s) 86, 226, 256, 349, 1306
TspDTI ATGAA 4 cut(s) 102, 1053, 1145, 1705
TspMI CCCGGG 1 cut(s) 278
TspRI CASTG 6 cut(s) 91, 259, 425, 1101, 1225, 1456
VneI GTGCAC 1 cut(s) 1736
VpaK11BI GGWCC 3 cut(s) 401, 530, 668
XagI CCTNNNNNAGG 1 cut(s) 511
XapI RAATTY 6 cut(s) 64, 959, 1041, 1146, 1169, 1406
XceI RCATGY 4 cut(s) 182, 497, 560, 1263
XmaI CCCGGG 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 450
XmnI GAANNNNTTC 1 cut(s) 875
XspI CTAG 7 cut(s) 183, 1031, 1161, 1281, 1485, 1512, 1730
Zsp2I ATGCAT 1 cut(s) 1722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.