pycom11g09990

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000610
Physical Location & Seq
Forward (+)
8369898 .. 8370900
1003 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g09990.1

Sequence Viewer

Length: 876 bp
ATGACTGCCAGGCCGTACGTGACTGGACTAGGAACCTTTGACAACTCTACTGTTGCTGGTATTTTAGAGTATGAATCAACCTCTAAAGCCCTCCACACTAAACTCCCAATGAAGAAGCTTCCGCTCTTCAAACCAATTCTTCCTGCTCTAAACGACACATCGTTTGCAACAAAGTTCAGCAACAAACTCCGTAGCTTAGCCAACGCACAATTCCCTGCTAATGTCCCCCAAAAGGTTGACAGACACTTTTTCTTCACAATAGGCCTTGGAACAAGCCCCTGCAGCAGCAATAACCAAACCTGCCAGGGACCTAATGGAACAATGTTTGCGGCCTCAGTTAATAACGTTTCTTTTGCAATGCCAACCACTGCTCTACTTCAAGCTCACTTCTCTGGGCAATCAAATGGCGTTTACAGCCCTGACTTCCCAAGCAGTCCTATAATCCCATTTAACTATACAGGCACCCCACCTAATGATACTATGGTGGGCGATGGTACAAAGCTAATGGTACTTCCATATAACACTAGTGTGGAGGTGGTCATGCAGGACACAAGCATTCTGGGCGCCGAAAGCCACCCTCTTCATCTGCATGGATTTAATTTCTTTGTTGTTGGCCAAGGGTTCGGGAACTTTGACCGGAATAAAGACCCTGCAAACTTCAATTTGGTTGACCCTGTTGAAAGAAATACCGTGGGTGTGCCCTCTGGTGGATGGGTTGCCATACGATTCCTAGCAGATAACCCCGGTGTATGGTTCATGCATTGCCATTTGGAGATCCACACTAGTTGGGGCTTGAAGATGGCTTGGCTGGTCCTAGATGGAAAGCTTCCCAACCAGAAGTTGCTTCCTCCCCCGGCGGATCTTCCCACCTGTTAA

Protein Analysis

292

Amino Acids

31.63

Weight (kDa)

7.11

Isoelectric Point (pI)

32.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_2 PF07731 143 - 273 3.5e-42 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000610)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60020 AT5G60020 AT5G60020
fragaria_vesca FvH4_2g09062 FvH4_2g09062 FvH4_3g05920 FvH4_3g34100 FvH4_3g34310 FvH4_3g34340 FvH4_6g13390
malus_domestica MD00G1015000.v1.1 MD03G1106500.v1.1 MD03G1106600.v1.1 MD04G1131500.v1.1 MD11G1118900.v1.1 MD11G1119000.v1.1 MD12G1144600.v1.1
prunus_persica Prupe.6G089100_v2.0.a1 Prupe.6G089300_v2.0.a1 Prupe.6G258000_v2.0.a1
pyrus_communis pycom03g08590 pycom10g02940 pycom11g09990 pycom11g10060
rosa_chinensis RchiOBHm_Chr2g0154491 RchiOBHm_Chr3g0466271 RchiOBHm_Chr5g0061891 RchiOBHm_Chr5g0061911 RchiOBHm_Chr5g0061951 RchiOBHm_Chr5g0061961 RchiOBHm_Chr6g0264371
rosa_laevigata RLG00000024591 RLG00000035514 RLG00000035516 RLG00000035518 RLG00000035519
rosa_multiflora Rmu_sc0003480.1_g000021 Rmu_sc0011641.1_g000002 Rmu_sc0011641.1_g000009
rosa_roxburghii Rroxscaffold_1G00018790 Rroxscaffold_1G00018820 Rroxscaffold_1G00018850 Rroxscaffold_2G00122520 Rroxscaffold_2G00122540 Rroxscaffold_6G00414660
rosa_rugosa Rorug03G0080700 Rorug04G0180900 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345100 Rorug05G0345200 Rorug06G0011800
rosa_samantha Rh3DG148900 Rh5AG405700 Rh5AG406100 Rh5AG406200 Rh5BG419200 Rh5BG419400 Rh5CG443100 Rh5CG443300 Rh5CG443500 Rh5CG443600 Rh5DG432700 Rh5DG434900 Rh6BG128700 Rh6CG127600 Rh6DG114200 Rh6DG114300
rosa_wichuraiana Rw3G011980 Rw5G038100 Rw5G038140 Rw5G038160 Rw5G038200 Rw5G038210 Rw5G038250 Rw5G038270 Rw5G038290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 308
AccB1I GGYRCC 2 cut(s) 461, 563
AccBSI CCGCTC 1 cut(s) 124
AciI CCGC 3 cut(s) 122, 329, 857
AclI AACGTT 1 cut(s) 345
AclWI GGATC 2 cut(s) 769, 867
AcoI YGGCCR 1 cut(s) 613
AcyI GRCGYC 1 cut(s) 564
AfaI GTAC 3 cut(s) 17, 496, 510
AfiI CCNNNNNNNGG 3 cut(s) 232, 707, 750
AgsI TTSAA 5 cut(s) 130, 380, 661, 680, 796
AhlI ACTAGT 2 cut(s) 524, 782
AjnI CCWGG 2 cut(s) 8, 303
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AleI CACNNNNGTG 1 cut(s) 527
AluBI AGCT 5 cut(s) 118, 195, 383, 502, 826
AluI AGCT 5 cut(s) 118, 195, 383, 502, 826
AlwI GGATC 2 cut(s) 769, 867
AoxI GGCC 4 cut(s) 11, 262, 330, 613
ApeKI GCWGC 2 cut(s) 282, 285
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AspLEI GCGC 1 cut(s) 566
AspS9I GGNCC 2 cut(s) 308, 811
AsuC2I CCSGG 2 cut(s) 744, 854
AvaII GGWCC 2 cut(s) 308, 811
BaeGI GKGCMC 1 cut(s) 702
BaeI ACNNNNGTAYC 2 cut(s) 468, 501
BalI TGGCCA 1 cut(s) 615
BanI GGYRCC 2 cut(s) 461, 563
BbvI GCAGC 2 cut(s) 294, 297
BccI CCATC 4 cut(s) 485, 705, 793, 812
BciT130I CCWGG 2 cut(s) 10, 305
BcnI CCSGG 2 cut(s) 744, 854
BcuI ACTAGT 2 cut(s) 524, 782
BfaI CTAG 5 cut(s) 29, 525, 731, 783, 815
BfmI CTRYAG 1 cut(s) 280
BfoI RGCGCY 1 cut(s) 567
BfuAI ACCTGC 1 cut(s) 308
BisI GCNGC 3 cut(s) 283, 286, 330
BlpI GCTNAGC 1 cut(s) 196
BlsI GCNGC 3 cut(s) 284, 287, 331
Bme1390I CCNGG 4 cut(s) 10, 305, 744, 854
Bme18I GGWCC 2 cut(s) 308, 811
BmgT120I GGNCC 2 cut(s) 308, 811
BmiI GGNNCC 4 cut(s) 34, 309, 463, 565
BmrFI CCNGG 4 cut(s) 10, 305, 744, 854
Bpu1102I GCTNAGC 1 cut(s) 196
BpuMI CCSGG 2 cut(s) 744, 854
BsaAI YACGTR 1 cut(s) 19
BsaHI GRCGYC 1 cut(s) 564
BsaJI CCNNGG 6 cut(s) 265, 304, 616, 690, 742, 852
BsaWI WCCGGW 1 cut(s) 636
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 707, 750
Bse1I ACTGG 1 cut(s) 28
Bse3DI GCAATG 2 cut(s) 363, 760
BseBI CCWGG 2 cut(s) 10, 305
BseDI CCNNGG 6 cut(s) 265, 304, 616, 690, 742, 852
BseGI GGATG 1 cut(s) 716
BseLI CCNNNNNNNGG 3 cut(s) 232, 707, 750
BseMI GCAATG 2 cut(s) 363, 760
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 1 cut(s) 28
BseSI GKGCMC 1 cut(s) 702
BseXI GCAGC 2 cut(s) 294, 297
BshFI GGCC 4 cut(s) 13, 264, 332, 615
BshNI GGYRCC 2 cut(s) 461, 563
BsiSI CCGG 3 cut(s) 637, 744, 854
BsiWI CGTACG 1 cut(s) 15
BslFI GGGAC 2 cut(s) 209, 321
BslI CCNNNNNNNGG 3 cut(s) 232, 707, 750
BsmFI GGGAC 2 cut(s) 209, 321
BsmI GAATGC 1 cut(s) 555
BsnI GGCC 4 cut(s) 13, 264, 332, 615
Bsp1286I GDGCHC 1 cut(s) 702
Bsp143I GATC 2 cut(s) 774, 859
Bsp1720I GCTNAGC 1 cut(s) 196
BspACI CCGC 3 cut(s) 122, 329, 857
BspANI GGCC 4 cut(s) 13, 264, 332, 615
BspCNI CTCAG 1 cut(s) 347
BspLI GGNNCC 4 cut(s) 34, 309, 463, 565
BspMAI CTGCAG 1 cut(s) 284
BspMI ACCTGC 1 cut(s) 308
BspPI GGATC 2 cut(s) 769, 867
BspQI GCTCTTC 1 cut(s) 131
BspT107I GGYRCC 2 cut(s) 461, 563
BsrBI CCGCTC 1 cut(s) 124
BsrDI GCAATG 2 cut(s) 363, 760
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 6 cut(s) 265, 304, 616, 690, 742, 852
BssMI GATC 2 cut(s) 774, 859
BssNI GRCGYC 1 cut(s) 564
BssT1I CCWWGG 2 cut(s) 265, 616
Bst2UI CCWGG 2 cut(s) 10, 305
Bst4CI ACNGT 2 cut(s) 52, 691
Bst6I CTCTTC 2 cut(s) 131, 585
BstACI GRCGYC 1 cut(s) 564
BstBAI YACGTR 1 cut(s) 19
BstDEI CTNAG 2 cut(s) 196, 334
BstDSI CCRYGG 1 cut(s) 690
BstF5I GGATG 1 cut(s) 716
BstH2I RGCGCY 1 cut(s) 567
BstHHI GCGC 1 cut(s) 566
BstKTI GATC 2 cut(s) 777, 862
BstMBI GATC 2 cut(s) 774, 859
BstMWI GCNNNNNNNGC 4 cut(s) 282, 414, 561, 570
BstNI CCWGG 2 cut(s) 10, 305
BstSCI CCNGG 4 cut(s) 8, 303, 742, 852
BstSFI CTRYAG 1 cut(s) 280
BstSLI GKGCMC 1 cut(s) 702
BstV1I GCAGC 2 cut(s) 294, 297
BstX2I RGATCY 2 cut(s) 774, 859
BstYI RGATCY 2 cut(s) 774, 859
BsuRI GGCC 4 cut(s) 13, 264, 332, 615
BtgI CCRYGG 1 cut(s) 690
BtgZI GCGATG 1 cut(s) 504
BtsCI GGATG 1 cut(s) 716
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 1 cut(s) 366
BveI ACCTGC 1 cut(s) 308
CfoI GCGC 1 cut(s) 566
Cfr13I GGNCC 2 cut(s) 308, 811
Csp6I GTAC 3 cut(s) 16, 495, 509
CspCI CAANNNNNGTGG 2 cut(s) 767, 802
CviAII CATG 3 cut(s) 541, 590, 757
CviQI GTAC 3 cut(s) 16, 495, 509
DdeI CTNAG 2 cut(s) 196, 334
DinI GGCGCC 1 cut(s) 565
DpnI GATC 2 cut(s) 776, 861
DpnII GATC 2 cut(s) 774, 859
EaeI YGGCCR 1 cut(s) 613
Eam1104I CTCTTC 2 cut(s) 131, 585
EarI CTCTTC 2 cut(s) 131, 585
EciI GGCGGA 1 cut(s) 872
Eco130I CCWWGG 2 cut(s) 265, 616
Eco147I AGGCCT 1 cut(s) 264
Eco47I GGWCC 2 cut(s) 308, 811
EcoO109I RGGNCCY 1 cut(s) 308
EcoRII CCWGG 2 cut(s) 8, 303
EcoT14I CCWWGG 2 cut(s) 265, 616
EcoT22I ATGCAT 1 cut(s) 762
EgeI GGCGCC 1 cut(s) 565
EheI GGCGCC 1 cut(s) 565
ErhI CCWWGG 2 cut(s) 265, 616
FaeI CATG 3 cut(s) 544, 593, 760
FaqI GGGAC 2 cut(s) 209, 321
FatI CATG 3 cut(s) 540, 589, 756
Fnu4HI GCNGC 3 cut(s) 283, 286, 330
FokI GGATG 1 cut(s) 723
Fsp4HI GCNGC 3 cut(s) 283, 286, 330
FspBI CTAG 5 cut(s) 29, 525, 731, 783, 815
GlaI GCGC 1 cut(s) 565
GluI GCNGC 3 cut(s) 283, 286, 330
HaeII RGCGCY 1 cut(s) 567
HaeIII GGCC 4 cut(s) 13, 264, 332, 615
HapII CCGG 3 cut(s) 637, 744, 854
HhaI GCGC 1 cut(s) 566
Hin1I GRCGYC 1 cut(s) 564
Hin1II CATG 3 cut(s) 544, 593, 760
Hin6I GCGC 1 cut(s) 564
HinP1I GCGC 1 cut(s) 564
HincII GTYRAC 2 cut(s) 238, 670
HindII GTYRAC 2 cut(s) 238, 670
HindIII AAGCTT 2 cut(s) 116, 824
HinfI GANTC 2 cut(s) 74, 726
HpaII CCGG 3 cut(s) 637, 744, 854
Hpy166II GTNNAC 3 cut(s) 238, 412, 670
Hpy188III TCNNGA 1 cut(s) 625
Hpy8I GTNNAC 3 cut(s) 238, 412, 670
HpyCH4III ACNGT 2 cut(s) 52, 691
HpyCH4IV ACGT 2 cut(s) 18, 345
HpyCH4V TGCA 7 cut(s) 167, 282, 356, 544, 589, 653, 760
HpyF10VI GCNNNNNNNGC 4 cut(s) 282, 414, 561, 570
HpyF3I CTNAG 2 cut(s) 196, 334
HpySE526I ACGT 2 cut(s) 18, 345
Hsp92I GRCGYC 1 cut(s) 564
Hsp92II CATG 3 cut(s) 544, 593, 760
HspAI GCGC 1 cut(s) 564
KasI GGCGCC 1 cut(s) 563
Kzo9I GATC 2 cut(s) 774, 859
LguI GCTCTTC 1 cut(s) 131
Lsp1109I GCAGC 2 cut(s) 294, 297
MaeI CTAG 5 cut(s) 29, 525, 731, 783, 815
MaeII ACGT 2 cut(s) 18, 345
MaeIII GTNAC 1 cut(s) 19
MalI GATC 2 cut(s) 776, 861
MbiI CCGCTC 1 cut(s) 124
MboI GATC 2 cut(s) 774, 859
MboII GAAGA 7 cut(s) 118, 124, 131, 244, 572, 808, 854
MflI RGATCY 2 cut(s) 774, 859
MhlI GDGCHC 1 cut(s) 702
MlsI TGGCCA 1 cut(s) 615
MluCI AATT 4 cut(s) 135, 209, 598, 661
MluNI TGGCCA 1 cut(s) 615
Mly113I GGCGCC 1 cut(s) 564
MnlI CCTC 7 cut(s) 91, 101, 343, 526, 588, 712, 858
Mox20I TGGCCA 1 cut(s) 615
Mph1103I ATGCAT 1 cut(s) 762
MscI TGGCCA 1 cut(s) 615
MseI TTAA 4 cut(s) 339, 450, 597, 874
MslI CAYNNNNRTG 2 cut(s) 527, 588
Msp20I TGGCCA 1 cut(s) 615
MspI CCGG 3 cut(s) 637, 744, 854
MspR9I CCNGG 4 cut(s) 10, 305, 744, 854
Mva1269I GAATGC 1 cut(s) 555
MvaI CCWGG 2 cut(s) 10, 305
MwoI GCNNNNNNNGC 4 cut(s) 282, 414, 561, 570
NarI GGCGCC 1 cut(s) 564
NciI CCSGG 2 cut(s) 744, 854
NdeII GATC 2 cut(s) 774, 859
NlaIII CATG 3 cut(s) 544, 593, 760
NlaIV GGNNCC 4 cut(s) 34, 309, 463, 565
NmuCI GTSAC 1 cut(s) 19
NsiI ATGCAT 1 cut(s) 762
OliI CACNNNNGTG 1 cut(s) 527
PceI AGGCCT 1 cut(s) 264
PciSI GCTCTTC 1 cut(s) 131
PctI GAATGC 1 cut(s) 555
PfeI GAWTC 2 cut(s) 74, 726
Pfl23II CGTACG 1 cut(s) 15
PkrI GCNGC 3 cut(s) 284, 287, 331
PluTI GGCGCC 1 cut(s) 567
Ppu21I YACGTR 1 cut(s) 19
PpuMI RGGWCCY 1 cut(s) 308
Psp1406I AACGTT 1 cut(s) 345
Psp5II RGGWCCY 1 cut(s) 308
Psp6I CCWGG 2 cut(s) 8, 303
PspGI CCWGG 2 cut(s) 8, 303
PspLI CGTACG 1 cut(s) 15
PspN4I GGNNCC 4 cut(s) 34, 309, 463, 565
PspPI GGNCC 2 cut(s) 308, 811
PspPPI RGGWCCY 1 cut(s) 308
PstI CTGCAG 1 cut(s) 284
PsuI RGATCY 2 cut(s) 774, 859
RsaI GTAC 3 cut(s) 17, 496, 510
RsaNI GTAC 3 cut(s) 16, 495, 509
RseI CAYNNNNRTG 2 cut(s) 527, 588
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 4 cut(s) 339, 450, 597, 874
SatI GCNGC 3 cut(s) 283, 286, 330
Sau3AI GATC 2 cut(s) 774, 859
Sau96I GGNCC 2 cut(s) 308, 811
ScrFI CCNGG 4 cut(s) 10, 305, 744, 854
SduI GDGCHC 1 cut(s) 702
SfcI CTRYAG 1 cut(s) 280
SfoI GGCGCC 1 cut(s) 565
SinI GGWCC 2 cut(s) 308, 811
SmiMI CAYNNNNRTG 2 cut(s) 527, 588
SpeI ACTAGT 2 cut(s) 524, 782
Sse9I AATT 4 cut(s) 135, 209, 598, 661
SseBI AGGCCT 1 cut(s) 264
SsiI CCGC 3 cut(s) 122, 329, 857
SspDI GGCGCC 1 cut(s) 563
SspMI CTAG 5 cut(s) 29, 525, 731, 783, 815
StuI AGGCCT 1 cut(s) 264
StyD4I CCNGG 4 cut(s) 8, 303, 742, 852
StyI CCWWGG 2 cut(s) 265, 616
TaaI ACNGT 2 cut(s) 52, 691
TaiI ACGT 2 cut(s) 21, 348
TasI AATT 4 cut(s) 135, 209, 598, 661
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 2 cut(s) 74, 726
Tru1I TTAA 4 cut(s) 339, 450, 597, 874
Tru9I TTAA 4 cut(s) 339, 450, 597, 874
TscAI CASTG 1 cut(s) 373
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 2 cut(s) 282, 285
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 4 cut(s) 87, 125, 572, 745
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 1 cut(s) 373
VpaK11BI GGWCC 2 cut(s) 308, 811
XcmI CCANNNNNNNNNTGG 1 cut(s) 311
XspI CTAG 5 cut(s) 29, 525, 731, 783, 815
Zsp2I ATGCAT 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.