Rroxscaffold_1G00018820

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000610
Physical Location & Seq
Forward (+)
22941242 .. 22943078
1837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00018820.1

Sequence Viewer

Length: 1170 bp
ATGAGAAATGATCGATCGAGCAACTCCTTCACTGCAAAGACTCATTCTTCCATATTAGAGAATGGTGGAAATGCAGATCCTGAAGCAGTCATAAACCAAGCCCTGCAAACCGGTGGAGGACCAAATGTTTCTGATGCTTACACCATCAACGGACTCCCAGGGCCATTGTACAACTGCTCTTCCAAAGATACTTTCAAGCTAAAGGTTCAAGCTGGGAAGACATACCTTCTGCGCCTAATCAATGCAGCGCTGAACGACGAGCTCTTCTTCAGCATTGCAAACCACACCCTCAAAGTGGTGGAAACTGATGCAGTTTACGTCAAACCCTTCGAGACCAACACCATTCTCATTGCCCCGGGACAAACCATAAATGTTCTTCTCAAAACAAAATCCAGCTACCCAAACGCCACATTTTTCATGACCGCAAGGCCATACGTGACTGGTCTAGGCACCTTTGACAACTCCACTCTTGCTGGAATTTTAGAATACGAAACGACTACACTTCATTCAACCCTCCCAATGAACAAACTAACACTCCACAAACCAATTCTCCCAGTTCTAAATGACACATCGTTTGCAACAAAATTCGCCAACAAACTCCGTAGCTTGAACACTCCCCAGTTCCCTGCTAATGTTCCACAGAAAGTTGACAAGCGTTTGTTCTTCACAATAGGGCTTGGAACCAACCCATGTAGCCAACAGAATCACAACAACCAGACTTGCCAGGGACCAAATGGAACCATGTTTGCTGCTTCAATCAATAATGTTTCTTTTGCTATGCCAAACACTGCCTTACTCCAATCCCACTTCTCTGGCCAATCTAAGGGGGTCTATTCGCCTGACTTTCCAATCAGTCCTGTAACTCCATTCAATTACACTGGGGCTCAGCTGAACAATACTAATGTGGGCAACGGTACAAAGCTAATGGTGCTTCCCTTTAATACTAGCGTGGAGTTGGTATTGCAGGACACTAGCATTCTTGGTGCTGAAAGTCATCCTCTTCATCTTCATGGATTCAATTTCTTTGTTGTTGGTCAAGGGTTTGGGAACTTCGACCGAAATAAGGATCCTAAGAAGTTCAATCTCATCGACCCCGTTGAGAGAAACACCGTGAGTGTGCCATCTGGTGGTTGGGTTGCTATTCGGCTTCTAGCAGACAACCCAGGTTAG

Protein Analysis

389

Amino Acids

42.2

Weight (kDa)

8.83

Isoelectric Point (pI)

27.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase PF00394 26 - 164 1.4e-38 Multicopper oxidase
Cu-oxidase_2 PF07731 282 - 389 1.3e-27 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000610)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60020 AT5G60020 AT5G60020
fragaria_vesca FvH4_2g09062 FvH4_2g09062 FvH4_3g05920 FvH4_3g34100 FvH4_3g34310 FvH4_3g34340 FvH4_6g13390
malus_domestica MD00G1015000.v1.1 MD03G1106500.v1.1 MD03G1106600.v1.1 MD04G1131500.v1.1 MD11G1118900.v1.1 MD11G1119000.v1.1 MD12G1144600.v1.1
prunus_persica Prupe.6G089100_v2.0.a1 Prupe.6G089300_v2.0.a1 Prupe.6G258000_v2.0.a1
pyrus_communis pycom03g08590 pycom10g02940 pycom11g09990 pycom11g10060
rosa_chinensis RchiOBHm_Chr2g0154491 RchiOBHm_Chr3g0466271 RchiOBHm_Chr5g0061891 RchiOBHm_Chr5g0061911 RchiOBHm_Chr5g0061951 RchiOBHm_Chr5g0061961 RchiOBHm_Chr6g0264371
rosa_laevigata RLG00000024591 RLG00000035514 RLG00000035516 RLG00000035518 RLG00000035519
rosa_multiflora Rmu_sc0003480.1_g000021 Rmu_sc0011641.1_g000002 Rmu_sc0011641.1_g000009
rosa_roxburghii Rroxscaffold_1G00018790 Rroxscaffold_1G00018820 Rroxscaffold_1G00018850 Rroxscaffold_2G00122520 Rroxscaffold_2G00122540 Rroxscaffold_6G00414660
rosa_rugosa Rorug03G0080700 Rorug04G0180900 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345100 Rorug05G0345200 Rorug06G0011800
rosa_samantha Rh3DG148900 Rh5AG405700 Rh5AG406100 Rh5AG406200 Rh5BG419200 Rh5BG419400 Rh5CG443100 Rh5CG443300 Rh5CG443500 Rh5CG443600 Rh5DG432700 Rh5DG434900 Rh6BG128700 Rh6CG127600 Rh6DG114200 Rh6DG114300
rosa_wichuraiana Rw3G011980 Rw5G038100 Rw5G038140 Rw5G038160 Rw5G038200 Rw5G038210 Rw5G038250 Rw5G038270 Rw5G038290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 449
AccB7I CCANNNNNTGG 1 cut(s) 1127
AciI CCGC 1 cut(s) 423
AclWI GGATC 3 cut(s) 71, 1061, 1074
AcoI YGGCCR 1 cut(s) 814
AcsI RAATTY 2 cut(s) 477, 584
AcuI CTGAAG 2 cut(s) 102, 253
AfaI GTAC 2 cut(s) 170, 916
AfeI AGCGCT 1 cut(s) 249
AfiI CCNNNNNNNGG 4 cut(s) 295, 823, 1063, 1127
AgeI ACCGGT 1 cut(s) 110
AgsI TTSAA 8 cut(s) 196, 209, 510, 610, 756, 871, 1018, 1081
AjnI CCWGG 3 cut(s) 157, 723, 1162
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AluBI AGCT 7 cut(s) 199, 212, 262, 396, 606, 889, 922
AluI AGCT 7 cut(s) 199, 212, 262, 396, 606, 889, 922
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 1 cut(s) 326
AlwI GGATC 3 cut(s) 71, 1061, 1074
AlwNI CAGNNNCTG 1 cut(s) 80
Ama87I CYCGRG 1 cut(s) 355
Aor51HI AGCGCT 1 cut(s) 249
AoxI GGCC 3 cut(s) 161, 428, 814
ApeKI GCWGC 2 cut(s) 245, 749
ApoI RAATTY 2 cut(s) 477, 584
ArsI GACNNNNNNTTYG 2 cut(s) 557, 589
AsiGI ACCGGT 1 cut(s) 110
AspLEI GCGC 2 cut(s) 234, 250
AspS9I GGNCC 3 cut(s) 119, 161, 728
AsuC2I CCSGG 2 cut(s) 356, 357
AvaI CYCGRG 1 cut(s) 355
AvaII GGWCC 2 cut(s) 119, 728
BalI TGGCCA 1 cut(s) 816
BamHI GGATCC 1 cut(s) 1066
BanI GGYRCC 1 cut(s) 449
BanII GRGCYC 2 cut(s) 264, 886
BbsI GAAGAC 1 cut(s) 224
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 2 cut(s) 257, 736
BccI CCATC 2 cut(s) 152, 1129
BciT130I CCWGG 3 cut(s) 159, 725, 1164
BcnI CCSGG 2 cut(s) 356, 357
BcoDI GTCTC 1 cut(s) 326
BfaI CTAG 4 cut(s) 446, 945, 972, 1151
BfoI RGCGCY 1 cut(s) 251
BisI GCNGC 2 cut(s) 246, 750
BlpI GCTNAGC 1 cut(s) 885
BlsI GCNGC 2 cut(s) 247, 751
Bme1390I CCNGG 5 cut(s) 159, 356, 357, 725, 1164
Bme18I GGWCC 2 cut(s) 119, 728
BmeT110I CYCGRG 1 cut(s) 355
BmgT120I GGNCC 3 cut(s) 119, 161, 728
BmiI GGNNCC 5 cut(s) 451, 682, 729, 739, 1068
BmrFI CCNGG 5 cut(s) 159, 356, 357, 725, 1164
BmrI ACTGGG 3 cut(s) 548, 613, 888
BmsI GCATC 2 cut(s) 124, 298
BmuI ACTGGG 3 cut(s) 548, 613, 888
BpiI GAAGAC 1 cut(s) 224
Bpu1102I GCTNAGC 1 cut(s) 885
BpuMI CCSGG 2 cut(s) 356, 357
Bsa29I ATCGAT 1 cut(s) 13
BsaAI YACGTR 1 cut(s) 436
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 6 cut(s) 157, 158, 354, 355, 724, 1162
BsaWI WCCGGW 1 cut(s) 110
BsaXI ACNNNNNCTCC 4 cut(s) 519, 534, 549, 564
Bsc4I CCNNNNNNNGG 4 cut(s) 295, 823, 1063, 1127
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 4 cut(s) 445, 554, 619, 883
Bse3DI GCAATG 2 cut(s) 273, 348
BseBI CCWGG 3 cut(s) 159, 725, 1164
BseCI ATCGAT 1 cut(s) 13
BseDI CCNNGG 6 cut(s) 157, 158, 354, 355, 724, 1162
BseGI GGATG 1 cut(s) 994
BseLI CCNNNNNNNGG 4 cut(s) 295, 823, 1063, 1127
BseMI GCAATG 2 cut(s) 273, 348
BseMII CTCAG 1 cut(s) 899
BseNI ACTGG 4 cut(s) 445, 554, 619, 883
BseXI GCAGC 2 cut(s) 257, 736
BseYI CCCAGC 1 cut(s) 212
Bsh1285I CGRYCG 2 cut(s) 17, 1057
BshFI GGCC 3 cut(s) 163, 430, 816
BshNI GGYRCC 1 cut(s) 449
BshTI ACCGGT 1 cut(s) 110
BshVI ATCGAT 1 cut(s) 13
BsiEI CGRYCG 2 cut(s) 17, 1057
BsiHKAI GWGCWC 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 355
BsiSI CCGG 2 cut(s) 111, 356
BslFI GGGAC 2 cut(s) 372, 741
BslI CCNNNNNNNGG 4 cut(s) 295, 823, 1063, 1127
BsmAI GTCTC 1 cut(s) 326
BsmFI GGGAC 2 cut(s) 372, 741
BsmI GAATGC 1 cut(s) 975
BsnI GGCC 3 cut(s) 163, 430, 816
Bso31I GGTCTC 1 cut(s) 326
BsoBI CYCGRG 1 cut(s) 355
Bsp1286I GDGCHC 2 cut(s) 264, 886
Bsp1407I TGTACA 1 cut(s) 168
Bsp143I GATC 4 cut(s) 10, 14, 76, 1066
Bsp1720I GCTNAGC 1 cut(s) 885
BspACI CCGC 1 cut(s) 423
BspANI GGCC 3 cut(s) 163, 430, 816
BspCNI CTCAG 1 cut(s) 898
BspDI ATCGAT 1 cut(s) 13
BspHI TCATGA 1 cut(s) 417
BspLI GGNNCC 5 cut(s) 451, 682, 729, 739, 1068
BspPI GGATC 3 cut(s) 71, 1061, 1074
BspQI GCTCTTC 2 cut(s) 184, 269
BspT107I GGYRCC 1 cut(s) 449
BspTNI GGTCTC 1 cut(s) 326
BsrDI GCAATG 2 cut(s) 273, 348
BsrFI RCCGGY 1 cut(s) 110
BsrGI TGTACA 1 cut(s) 168
BsrI ACTGG 4 cut(s) 445, 554, 619, 883
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 6 cut(s) 157, 158, 354, 355, 724, 1162
BssMI GATC 4 cut(s) 10, 14, 76, 1066
Bst2UI CCWGG 3 cut(s) 159, 725, 1164
Bst4CI ACNGT 2 cut(s) 914, 1111
Bst6I CTCTTC 3 cut(s) 184, 269, 1005
BstAUI TGTACA 1 cut(s) 168
BstBAI YACGTR 1 cut(s) 436
BstDEI CTNAG 3 cut(s) 822, 885, 1071
BstF5I GGATG 1 cut(s) 994
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 2 cut(s) 234, 250
BstKTI GATC 4 cut(s) 13, 17, 79, 1069
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 4 cut(s) 10, 14, 76, 1066
BstMCI CGRYCG 2 cut(s) 17, 1057
BstMWI GCNNNNNNNGC 1 cut(s) 928
BstNI CCWGG 3 cut(s) 159, 725, 1164
BstSCI CCNGG 5 cut(s) 157, 354, 355, 723, 1162
BstV1I GCAGC 2 cut(s) 257, 736
BstV2I GAAGAC 1 cut(s) 224
BstX2I RGATCY 2 cut(s) 76, 1066
BstXI CCANNNNNNTGG 1 cut(s) 812
BstYI RGATCY 2 cut(s) 76, 1066
Bsu15I ATCGAT 1 cut(s) 13
BsuRI GGCC 3 cut(s) 163, 430, 816
BsuTUI ATCGAT 1 cut(s) 13
BtsCI GGATG 1 cut(s) 994
BtsI GCAGTG 2 cut(s) 30, 786
BtsIMutI CAGTG 3 cut(s) 30, 786, 876
CaiI CAGNNNCTG 1 cut(s) 80
CciI TCATGA 1 cut(s) 417
CfoI GCGC 2 cut(s) 234, 250
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 3 cut(s) 119, 161, 728
Cfr9I CCCGGG 1 cut(s) 355
ClaI ATCGAT 1 cut(s) 13
Csp6I GTAC 2 cut(s) 169, 915
CspAI ACCGGT 1 cut(s) 110
CviAII CATG 4 cut(s) 418, 690, 742, 1010
CviQI GTAC 2 cut(s) 169, 915
DdeI CTNAG 3 cut(s) 822, 885, 1071
DpnI GATC 4 cut(s) 12, 16, 78, 1068
DpnII GATC 4 cut(s) 10, 14, 76, 1066
EaeI YGGCCR 1 cut(s) 814
Eam1104I CTCTTC 3 cut(s) 184, 269, 1005
EarI CTCTTC 3 cut(s) 184, 269, 1005
Ecl136II GAGCTC 1 cut(s) 262
Eco24I GRGCYC 2 cut(s) 264, 886
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 2 cut(s) 119, 728
Eco47III AGCGCT 1 cut(s) 249
Eco53kI GAGCTC 1 cut(s) 262
Eco57I CTGAAG 2 cut(s) 102, 253
Eco88I CYCGRG 1 cut(s) 355
EcoICRI GAGCTC 1 cut(s) 262
EcoRII CCWGG 3 cut(s) 157, 723, 1162
EcoT38I GRGCYC 2 cut(s) 264, 886
FaeI CATG 4 cut(s) 421, 693, 745, 1013
FaqI GGGAC 2 cut(s) 372, 741
FatI CATG 4 cut(s) 417, 689, 741, 1009
Fnu4HI GCNGC 2 cut(s) 246, 750
FokI GGATG 1 cut(s) 981
FriOI GRGCYC 2 cut(s) 264, 886
Fsp4HI GCNGC 2 cut(s) 246, 750
FspBI CTAG 4 cut(s) 446, 945, 972, 1151
GlaI GCGC 2 cut(s) 233, 249
GluI GCNGC 2 cut(s) 246, 750
GsaI CCCAGC 1 cut(s) 216
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 3 cut(s) 163, 430, 816
HapII CCGG 2 cut(s) 111, 356
HhaI GCGC 2 cut(s) 234, 250
Hin1II CATG 4 cut(s) 421, 693, 745, 1013
Hin6I GCGC 2 cut(s) 232, 248
HinP1I GCGC 2 cut(s) 232, 248
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HinfI GANTC 4 cut(s) 40, 153, 703, 1014
HpaII CCGG 2 cut(s) 111, 356
Hpy166II GTNNAC 2 cut(s) 316, 649
Hpy188I TCNGA 1 cut(s) 133
Hpy188III TCNNGA 3 cut(s) 80, 331, 418
Hpy8I GTNNAC 2 cut(s) 316, 649
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 3 cut(s) 37, 236, 337
HpyCH4III ACNGT 2 cut(s) 914, 1111
HpyCH4IV ACGT 2 cut(s) 318, 435
HpyCH4V TGCA 8 cut(s) 35, 74, 106, 245, 278, 311, 578, 964
HpyF10VI GCNNNNNNNGC 1 cut(s) 928
HpyF3I CTNAG 3 cut(s) 822, 885, 1071
HpySE526I ACGT 2 cut(s) 318, 435
Hsp92II CATG 4 cut(s) 421, 693, 745, 1013
HspAI GCGC 2 cut(s) 232, 248
Kzo9I GATC 4 cut(s) 10, 14, 76, 1066
LguI GCTCTTC 2 cut(s) 184, 269
Lsp1109I GCAGC 2 cut(s) 257, 736
LweI GCATC 2 cut(s) 124, 298
MaeI CTAG 4 cut(s) 446, 945, 972, 1151
MaeII ACGT 2 cut(s) 318, 435
MaeIII GTNAC 2 cut(s) 436, 859
MalI GATC 4 cut(s) 12, 16, 78, 1068
MboI GATC 4 cut(s) 10, 14, 76, 1066
MboII GAAGA 9 cut(s) 39, 171, 229, 256, 259, 368, 655, 992, 998
MflI RGATCY 2 cut(s) 76, 1066
MhlI GDGCHC 2 cut(s) 264, 886
MlsI TGGCCA 1 cut(s) 816
MluCI AATT 5 cut(s) 477, 546, 584, 871, 1018
MluNI TGGCCA 1 cut(s) 816
MlyI GAGTC 2 cut(s) 34, 147
MnlI CCTC 4 cut(s) 110, 299, 524, 1008
Mox20I TGGCCA 1 cut(s) 816
MscI TGGCCA 1 cut(s) 816
MseI TTAA 1 cut(s) 939
MslI CAYNNNNRTG 1 cut(s) 1008
Msp20I TGGCCA 1 cut(s) 816
MspA1I CMGCKG 1 cut(s) 889
MspI CCGG 2 cut(s) 111, 356
MspR9I CCNGG 5 cut(s) 159, 356, 357, 725, 1164
Mva1269I GAATGC 1 cut(s) 975
MvaI CCWGG 3 cut(s) 159, 725, 1164
MwoI GCNNNNNNNGC 1 cut(s) 928
NciI CCSGG 2 cut(s) 356, 357
NdeII GATC 4 cut(s) 10, 14, 76, 1066
NlaIII CATG 4 cut(s) 421, 693, 745, 1013
NlaIV GGNNCC 5 cut(s) 451, 682, 729, 739, 1068
NmuCI GTSAC 1 cut(s) 436
PagI TCATGA 1 cut(s) 417
PasI CCCWGGG 1 cut(s) 158
PciSI GCTCTTC 2 cut(s) 184, 269
PctI GAATGC 1 cut(s) 975
PfeI GAWTC 2 cut(s) 703, 1014
PflMI CCANNNNNTGG 1 cut(s) 1127
PinAI ACCGGT 1 cut(s) 110
PkrI GCNGC 2 cut(s) 247, 751
Ple19I CGATCG 1 cut(s) 17
PleI GAGTC 2 cut(s) 34, 147
PpsI GAGTC 2 cut(s) 34, 147
Ppu21I YACGTR 1 cut(s) 436
Psp124BI GAGCTC 1 cut(s) 264
Psp6I CCWGG 3 cut(s) 157, 723, 1162
PspFI CCCAGC 1 cut(s) 212
PspGI CCWGG 3 cut(s) 157, 723, 1162
PspN4I GGNNCC 5 cut(s) 451, 682, 729, 739, 1068
PspPI GGNCC 3 cut(s) 119, 161, 728
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 2 cut(s) 76, 1066
PvuI CGATCG 1 cut(s) 17
PvuII CAGCTG 1 cut(s) 889
RsaI GTAC 2 cut(s) 170, 916
RsaNI GTAC 2 cut(s) 169, 915
RseI CAYNNNNRTG 1 cut(s) 1008
SacI GAGCTC 1 cut(s) 264
SapI GCTCTTC 2 cut(s) 184, 269
SaqAI TTAA 1 cut(s) 939
SatI GCNGC 2 cut(s) 246, 750
Sau3AI GATC 4 cut(s) 10, 14, 76, 1066
Sau96I GGNCC 3 cut(s) 119, 161, 728
SchI GAGTC 2 cut(s) 34, 147
ScrFI CCNGG 5 cut(s) 159, 356, 357, 725, 1164
SduI GDGCHC 2 cut(s) 264, 886
SfaNI GCATC 2 cut(s) 124, 298
SinI GGWCC 2 cut(s) 119, 728
SmaI CCCGGG 1 cut(s) 357
SmiMI CAYNNNNRTG 1 cut(s) 1008
Sse9I AATT 5 cut(s) 477, 546, 584, 871, 1018
SsiI CCGC 1 cut(s) 423
SspMI CTAG 4 cut(s) 446, 945, 972, 1151
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 5 cut(s) 157, 354, 355, 723, 1162
TaaI ACNGT 2 cut(s) 914, 1111
TaiI ACGT 2 cut(s) 321, 438
TaqI TCGA 5 cut(s) 13, 17, 330, 1053, 1089
TaqII GACCGA 1 cut(s) 1071
TasI AATT 5 cut(s) 477, 546, 584, 871, 1018
TatI WGTACW 1 cut(s) 168
TfiI GAWTC 2 cut(s) 703, 1014
Tru1I TTAA 1 cut(s) 939
Tru9I TTAA 1 cut(s) 939
TscAI CASTG 3 cut(s) 37, 793, 883
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 2 cut(s) 245, 749
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 5 cut(s) 406, 494, 536, 992, 998
TspGWI ACGGA 2 cut(s) 165, 590
TspMI CCCGGG 1 cut(s) 355
TspRI CASTG 3 cut(s) 37, 793, 883
Van91I CCANNNNNTGG 1 cut(s) 1127
VpaK11BI GGWCC 2 cut(s) 119, 728
XapI RAATTY 2 cut(s) 477, 584
XcmI CCANNNNNNNNNTGG 2 cut(s) 731, 1128
XmaI CCCGGG 1 cut(s) 355
XspI CTAG 4 cut(s) 446, 945, 972, 1151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.