RLG00000035516

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000610
Physical Location & Seq
Reverse (-)
67909034 .. 67911891
2858 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035516

Sequence Viewer

Length: 1620 bp
ATGGGGGTTGCTCTTCTTTCAGTTGTTAAGCTCTTTGTTTTCTCTTTCATCACATTGGGTCTCATTCCTCAGCAGGCTCTTGCAAGCATAACGAGACATTACAAGTTTGATATCCAAATGAAAAATGTGACAAGATTGTGTCACACGAAGAGCATTGTAACTGTAAACGGTCAATTTCCTGGGCCTCGAATTGTTGCTAGGGAGGGTGATCGCCTCCTCATTAAAGTGGTTAACCATGTTCAAAGCAACATCTCCATCCACTGGCATGGGATTCGACAACTTCAAACTGGATGGGCAGATGGTCCAGCATACATTACCCAATGTCCAATACAATCTGGCAACGGTTATGTGTACAACTTCACCATTATAGGCCAAAGAGGCACTCTTTGGTGGCATGCTCACATTTCATGGCTAAGATCAACCATCTATGGTCCCTTAATCATTCTTCCCAAGCGTGGTGTTCCTTATCCATTTACCAAACCCTACACGGAAGTTCCCATCATCTTTGGAGAATGGTGGAATGCAGATCCTGAAGCAGTCATAAACCAAGCTCTGCAAACCGGTGGAGGACCAAATGTTTCTGATGCTTACACCATCAACGGACTCCCAGGGCCATTGTACAACTGCTCTTCCAAAGATACTTTCAAGCTAAAGGTTCAAGCTGGGAAGACATACCTTCTGCGCCTAATCAATGCAGCACTGAACGATGAGCTCTTCTTCAGCATTGCAAACCACACCCTCAAAGTGGTGGAAACTGATGCAGTTTACGTCAAACCCTTCGAGACCAACACCATTCTCATTGCCCCGGGACAAACCATTAATGTTCTTCTCAAAACAAAACCCAGCTACCCAAACGCCACATTTTTCATGACCGCAAGGCCATACGTGACTGGTCTAGGCACCTTTGACAACTCCACTTTTGCTGGAATTTTAGAATACGAAACGACTACACTTCATTCAACCCTCCCAATGAACAAACTTCCACTCCACAAGCCAATTCTCCCAGTTCTAAATGATACATCGTTTGCAACAAAATTCGCCAACAAACTCCGTAGCTTGAACACTCCCCAGTTCCCAGCTAATGTTCCACAGAAAGTTGACAAGCGTTTGTTCTTCACAATAGGGCTTGGAACCAACCCATGTAGCCAACAGAATCACAACAACCAGACTTGCCAGGGACCAAATGGAACCATGTTTGCTGCTTCAATCAATAATGTTTCTTTTGCTATGCCAAACACTGCCTTACTCCAATCCCACTTCTCTGGCCAATCTAAGGGGGTCTATTCGCCTGACTTTCCAATCAGTCCTGTAACTCCGTTCAATTACACTGGGGCTCAGCTGAACAATACTAATGTGGGCAACGGTACAAAGCTAATGGTGCTTCCCTTTAATACTAGCGTGGAGTTGGTGTTGCAGGACACTAGTATTCTTGGTGCTGAAAGTCATCCCCTTCATCTTCATGGATTCAATTTCTTTGTTGTTGGTCAAGGGTTTGGGAACTTCGACCAAAATAAGGACCCTAAGAAGTTCAATCTCATCGACCCCGTTGAGAGAAACACCGTGGGTGTGCCATCCGGTGGTTGGGTTGCTATTCGGTTTCTAGCAGACAACCCAGGTTAG

Protein Analysis

540

Amino Acids

59.43

Weight (kDa)

9.34

Isoelectric Point (pI)

30.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 38 - 151 5.8e-42 Multicopper oxidase
Cu-oxidase PF00394 164 - 314 3.5e-43 Multicopper oxidase
Cu-oxidase_2 PF07731 432 - 539 2.7e-28 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000610)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60020 AT5G60020 AT5G60020
fragaria_vesca FvH4_2g09062 FvH4_2g09062 FvH4_3g05920 FvH4_3g34100 FvH4_3g34310 FvH4_3g34340 FvH4_6g13390
malus_domestica MD00G1015000.v1.1 MD03G1106500.v1.1 MD03G1106600.v1.1 MD04G1131500.v1.1 MD11G1118900.v1.1 MD11G1119000.v1.1 MD12G1144600.v1.1
prunus_persica Prupe.6G089100_v2.0.a1 Prupe.6G089300_v2.0.a1 Prupe.6G258000_v2.0.a1
pyrus_communis pycom03g08590 pycom10g02940 pycom11g09990 pycom11g10060
rosa_chinensis RchiOBHm_Chr2g0154491 RchiOBHm_Chr3g0466271 RchiOBHm_Chr5g0061891 RchiOBHm_Chr5g0061911 RchiOBHm_Chr5g0061951 RchiOBHm_Chr5g0061961 RchiOBHm_Chr6g0264371
rosa_laevigata RLG00000024591 RLG00000035514 RLG00000035516 RLG00000035518 RLG00000035519
rosa_multiflora Rmu_sc0003480.1_g000021 Rmu_sc0011641.1_g000002 Rmu_sc0011641.1_g000009
rosa_roxburghii Rroxscaffold_1G00018790 Rroxscaffold_1G00018820 Rroxscaffold_1G00018850 Rroxscaffold_2G00122520 Rroxscaffold_2G00122540 Rroxscaffold_6G00414660
rosa_rugosa Rorug03G0080700 Rorug04G0180900 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345100 Rorug05G0345200 Rorug06G0011800
rosa_samantha Rh3DG148900 Rh5AG405700 Rh5AG406100 Rh5AG406200 Rh5BG419200 Rh5BG419400 Rh5CG443100 Rh5CG443300 Rh5CG443500 Rh5CG443600 Rh5DG432700 Rh5DG434900 Rh6BG128700 Rh6CG127600 Rh6DG114200 Rh6DG114300
rosa_wichuraiana Rw3G011980 Rw5G038100 Rw5G038140 Rw5G038160 Rw5G038200 Rw5G038210 Rw5G038250 Rw5G038270 Rw5G038290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 899
AccB7I CCANNNNNTGG 2 cut(s) 261, 1577
AciI CCGC 1 cut(s) 873
AclWI GGATC 1 cut(s) 521
AcoI YGGCCR 1 cut(s) 1264
AcsI RAATTY 2 cut(s) 927, 1034
AcuI CTGAAG 2 cut(s) 552, 703
AfaI GTAC 3 cut(s) 353, 620, 1366
AfiI CCNNNNNNNGG 7 cut(s) 261, 455, 745, 1273, 1513, 1577, 1581
AgeI ACCGGT 1 cut(s) 560
AhlI ACTAGT 1 cut(s) 1421
AjnI CCWGG 4 cut(s) 178, 607, 1173, 1612
AjuI GAANNNNNNNTTGG 2 cut(s) 626, 658
Alw21I GWGCWC 1 cut(s) 714
Alw26I GTCTC 3 cut(s) 65, 88, 776
AlwI GGATC 1 cut(s) 521
AlwNI CAGNNNCTG 1 cut(s) 530
Ama87I CYCGRG 1 cut(s) 805
AoxI GGCC 5 cut(s) 182, 370, 611, 878, 1264
ApeKI GCWGC 2 cut(s) 695, 1199
ApoI RAATTY 2 cut(s) 927, 1034
AseI ATTAAT 1 cut(s) 819
AsiGI ACCGGT 1 cut(s) 560
AspLEI GCGC 1 cut(s) 684
AspS9I GGNCC 7 cut(s) 182, 302, 431, 569, 611, 1178, 1516
AsuC2I CCSGG 2 cut(s) 806, 807
AsuHPI GGTGA 2 cut(s) 218, 352
AvaI CYCGRG 1 cut(s) 805
AvaII GGWCC 5 cut(s) 302, 431, 569, 1178, 1516
BalI TGGCCA 1 cut(s) 1266
BanI GGYRCC 1 cut(s) 899
BanII GRGCYC 2 cut(s) 714, 1336
BbsI GAAGAC 1 cut(s) 674
Bbv12I GWGCWC 1 cut(s) 714
BbvCI CCTCAGC 1 cut(s) 69
BbvI GCAGC 2 cut(s) 707, 1186
BccI CCATC 7 cut(s) 263, 285, 293, 431, 506, 602, 1579
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 4 cut(s) 180, 609, 1175, 1614
BcnI CCSGG 2 cut(s) 806, 807
BcoDI GTCTC 3 cut(s) 65, 88, 776
BcuI ACTAGT 1 cut(s) 1421
BfaI CTAG 5 cut(s) 198, 896, 1395, 1422, 1601
BglI GCCNNNNNGGC 1 cut(s) 378
BisI GCNGC 2 cut(s) 696, 1200
BlpI GCTNAGC 1 cut(s) 1335
BlsI GCNGC 2 cut(s) 697, 1201
Bme1390I CCNGG 6 cut(s) 180, 609, 806, 807, 1175, 1614
Bme18I GGWCC 5 cut(s) 302, 431, 569, 1178, 1516
BmeT110I CYCGRG 1 cut(s) 805
BmgT120I GGNCC 7 cut(s) 182, 302, 431, 569, 611, 1178, 1516
BmiI GGNNCC 6 cut(s) 433, 901, 1132, 1179, 1189, 1518
BmrFI CCNGG 6 cut(s) 180, 609, 806, 807, 1175, 1614
BmrI ACTGGG 3 cut(s) 998, 1063, 1338
BmsI GCATC 2 cut(s) 574, 748
BmuI ACTGGG 3 cut(s) 998, 1063, 1338
BpiI GAAGAC 1 cut(s) 674
Bpu10I CCTNAGC 1 cut(s) 69
Bpu1102I GCTNAGC 1 cut(s) 1335
BpuMI CCSGG 2 cut(s) 806, 807
BsaAI YACGTR 1 cut(s) 886
BsaI GGTCTC 2 cut(s) 65, 776
BsaJI CCNNGG 8 cut(s) 179, 607, 608, 804, 805, 1174, 1560, 1612
BsaWI WCCGGW 2 cut(s) 560, 1574
BsaXI ACNNNNNCTCC 4 cut(s) 969, 999, 1394, 1424
Bsc4I CCNNNNNNNGG 7 cut(s) 261, 455, 745, 1273, 1513, 1577, 1581
Bse118I RCCGGY 1 cut(s) 560
Bse1I ACTGG 6 cut(s) 266, 292, 895, 1004, 1069, 1333
Bse3DI GCAATG 2 cut(s) 723, 798
BseBI CCWGG 4 cut(s) 180, 609, 1175, 1614
BseDI CCNNGG 8 cut(s) 179, 607, 608, 804, 805, 1174, 1560, 1612
BseGI GGATG 4 cut(s) 255, 296, 1444, 1571
BseLI CCNNNNNNNGG 7 cut(s) 261, 455, 745, 1273, 1513, 1577, 1581
BseMI GCAATG 2 cut(s) 723, 798
BseMII CTCAG 2 cut(s) 83, 1349
BseNI ACTGG 6 cut(s) 266, 292, 895, 1004, 1069, 1333
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 2 cut(s) 707, 1186
BseYI CCCAGC 3 cut(s) 662, 842, 1075
BshFI GGCC 5 cut(s) 184, 372, 613, 880, 1266
BshNI GGYRCC 1 cut(s) 899
BshTI ACCGGT 1 cut(s) 560
BsiHKAI GWGCWC 1 cut(s) 714
BsiHKCI CYCGRG 1 cut(s) 805
BsiSI CCGG 3 cut(s) 561, 806, 1575
BslFI GGGAC 3 cut(s) 417, 822, 1191
BslI CCNNNNNNNGG 7 cut(s) 261, 455, 745, 1273, 1513, 1577, 1581
BsmAI GTCTC 3 cut(s) 65, 88, 776
BsmFI GGGAC 3 cut(s) 417, 822, 1191
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 5 cut(s) 184, 372, 613, 880, 1266
Bso31I GGTCTC 2 cut(s) 65, 776
BsoBI CYCGRG 1 cut(s) 805
Bsp1286I GDGCHC 2 cut(s) 714, 1336
Bsp1407I TGTACA 2 cut(s) 351, 618
Bsp143I GATC 3 cut(s) 208, 416, 526
Bsp1720I GCTNAGC 1 cut(s) 1335
BspACI CCGC 1 cut(s) 873
BspANI GGCC 5 cut(s) 184, 372, 613, 880, 1266
BspCNI CTCAG 2 cut(s) 82, 1348
BspHI TCATGA 1 cut(s) 867
BspLI GGNNCC 6 cut(s) 433, 901, 1132, 1179, 1189, 1518
BspPI GGATC 1 cut(s) 521
BspQI GCTCTTC 4 cut(s) 18, 143, 634, 719
BspT107I GGYRCC 1 cut(s) 899
BspTNI GGTCTC 2 cut(s) 65, 776
BsrDI GCAATG 2 cut(s) 723, 798
BsrFI RCCGGY 1 cut(s) 560
BsrGI TGTACA 2 cut(s) 351, 618
BsrI ACTGG 6 cut(s) 266, 292, 895, 1004, 1069, 1333
BssAI RCCGGY 1 cut(s) 560
BssECI CCNNGG 8 cut(s) 179, 607, 608, 804, 805, 1174, 1560, 1612
BssMI GATC 3 cut(s) 208, 416, 526
Bst2UI CCWGG 4 cut(s) 180, 609, 1175, 1614
Bst4CI ACNGT 5 cut(s) 163, 170, 344, 1364, 1561
Bst6I CTCTTC 4 cut(s) 18, 143, 634, 719
BstAUI TGTACA 2 cut(s) 351, 618
BstBAI YACGTR 1 cut(s) 886
BstC8I GCNNGC 3 cut(s) 75, 85, 396
BstDEI CTNAG 5 cut(s) 69, 413, 1272, 1335, 1521
BstDSI CCRYGG 1 cut(s) 1560
BstF5I GGATG 4 cut(s) 255, 296, 1444, 1571
BstHHI GCGC 1 cut(s) 684
BstKTI GATC 3 cut(s) 211, 419, 529
BstMAI GTCTC 3 cut(s) 65, 88, 776
BstMBI GATC 3 cut(s) 208, 416, 526
BstMWI GCNNNNNNNGC 2 cut(s) 378, 1378
BstNI CCWGG 4 cut(s) 180, 609, 1175, 1614
BstNSI RCATGY 1 cut(s) 398
BstSCI CCNGG 6 cut(s) 178, 607, 804, 805, 1173, 1612
BstV1I GCAGC 2 cut(s) 707, 1186
BstV2I GAAGAC 1 cut(s) 674
BstX2I RGATCY 1 cut(s) 526
BstXI CCANNNNNNTGG 2 cut(s) 266, 1262
BstYI RGATCY 1 cut(s) 526
BsuRI GGCC 5 cut(s) 184, 372, 613, 880, 1266
BtgI CCRYGG 1 cut(s) 1560
BtsCI GGATG 4 cut(s) 255, 296, 1444, 1571
BtsI GCAGTG 1 cut(s) 1236
BtsIMutI CAGTG 4 cut(s) 259, 698, 1236, 1326
Cac8I GCNNGC 3 cut(s) 75, 85, 396
CaiI CAGNNNCTG 1 cut(s) 530
CciI TCATGA 1 cut(s) 867
CfoI GCGC 1 cut(s) 684
Cfr10I RCCGGY 1 cut(s) 560
Cfr13I GGNCC 7 cut(s) 182, 302, 431, 569, 611, 1178, 1516
Cfr9I CCCGGG 1 cut(s) 805
Csp6I GTAC 3 cut(s) 352, 619, 1365
CspAI ACCGGT 1 cut(s) 560
CviAII CATG 8 cut(s) 236, 266, 395, 408, 868, 1140, 1192, 1460
CviQI GTAC 3 cut(s) 352, 619, 1365
DdeI CTNAG 5 cut(s) 69, 413, 1272, 1335, 1521
DpnI GATC 3 cut(s) 210, 418, 528
DpnII GATC 3 cut(s) 208, 416, 526
EaeI YGGCCR 1 cut(s) 1264
Eam1104I CTCTTC 4 cut(s) 18, 143, 634, 719
EarI CTCTTC 4 cut(s) 18, 143, 634, 719
Ecl136II GAGCTC 1 cut(s) 712
Eco24I GRGCYC 2 cut(s) 714, 1336
Eco31I GGTCTC 2 cut(s) 65, 776
Eco32I GATATC 1 cut(s) 112
Eco47I GGWCC 5 cut(s) 302, 431, 569, 1178, 1516
Eco53kI GAGCTC 1 cut(s) 712
Eco57I CTGAAG 2 cut(s) 552, 703
Eco88I CYCGRG 1 cut(s) 805
EcoICRI GAGCTC 1 cut(s) 712
EcoO109I RGGNCCY 1 cut(s) 1516
EcoRII CCWGG 4 cut(s) 178, 607, 1173, 1612
EcoRV GATATC 1 cut(s) 112
EcoT38I GRGCYC 2 cut(s) 714, 1336
FaeI CATG 8 cut(s) 239, 269, 398, 411, 871, 1143, 1195, 1463
FaqI GGGAC 3 cut(s) 417, 822, 1191
FatI CATG 8 cut(s) 235, 265, 394, 407, 867, 1139, 1191, 1459
Fnu4HI GCNGC 2 cut(s) 696, 1200
FokI GGATG 4 cut(s) 242, 303, 1431, 1558
FriOI GRGCYC 2 cut(s) 714, 1336
Fsp4HI GCNGC 2 cut(s) 696, 1200
FspBI CTAG 5 cut(s) 198, 896, 1395, 1422, 1601
GlaI GCGC 1 cut(s) 683
GluI GCNGC 2 cut(s) 696, 1200
GsaI CCCAGC 3 cut(s) 666, 846, 1079
HaeIII GGCC 5 cut(s) 184, 372, 613, 880, 1266
HapII CCGG 3 cut(s) 561, 806, 1575
HhaI GCGC 1 cut(s) 684
Hin1II CATG 8 cut(s) 239, 269, 398, 411, 871, 1143, 1195, 1463
Hin6I GCGC 1 cut(s) 682
HinP1I GCGC 1 cut(s) 682
HincII GTYRAC 2 cut(s) 232, 1099
HindII GTYRAC 2 cut(s) 232, 1099
HinfI GANTC 4 cut(s) 271, 603, 1153, 1464
HpaI GTTAAC 1 cut(s) 232
HpaII CCGG 3 cut(s) 561, 806, 1575
HphI GGTGA 2 cut(s) 218, 352
Hpy166II GTNNAC 5 cut(s) 166, 232, 352, 766, 1099
Hpy188I TCNGA 1 cut(s) 583
Hpy188III TCNNGA 3 cut(s) 530, 781, 868
Hpy8I GTNNAC 5 cut(s) 166, 232, 352, 766, 1099
HpyAV CCTTC 3 cut(s) 686, 787, 1460
HpyCH4III ACNGT 5 cut(s) 163, 170, 344, 1364, 1561
HpyCH4IV ACGT 2 cut(s) 768, 885
HpyCH4V TGCA 8 cut(s) 83, 524, 556, 695, 728, 761, 1028, 1414
HpyF10VI GCNNNNNNNGC 2 cut(s) 378, 1378
HpyF3I CTNAG 5 cut(s) 69, 413, 1272, 1335, 1521
HpySE526I ACGT 2 cut(s) 768, 885
Hsp92II CATG 8 cut(s) 239, 269, 398, 411, 871, 1143, 1195, 1463
HspAI GCGC 1 cut(s) 682
KspAI GTTAAC 1 cut(s) 232
Kzo9I GATC 3 cut(s) 208, 416, 526
LguI GCTCTTC 4 cut(s) 18, 143, 634, 719
Lsp1109I GCAGC 2 cut(s) 707, 1186
LweI GCATC 2 cut(s) 574, 748
MaeI CTAG 5 cut(s) 198, 896, 1395, 1422, 1601
MaeII ACGT 2 cut(s) 768, 885
MaeIII GTNAC 5 cut(s) 127, 140, 157, 886, 1309
MalI GATC 3 cut(s) 210, 418, 528
MboI GATC 3 cut(s) 208, 416, 526
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 2 cut(s) 714, 1336
MlsI TGGCCA 1 cut(s) 1266
MluCI AATT 7 cut(s) 173, 189, 927, 996, 1034, 1321, 1468
MluNI TGGCCA 1 cut(s) 1266
MlyI GAGTC 1 cut(s) 597
MnlI CCTC 9 cut(s) 78, 195, 196, 224, 227, 371, 560, 749, 974
Mox20I TGGCCA 1 cut(s) 1266
MscI TGGCCA 1 cut(s) 1266
MseI TTAA 6 cut(s) 27, 222, 231, 437, 819, 1389
MslI CAYNNNNRTG 3 cut(s) 224, 264, 1458
Msp20I TGGCCA 1 cut(s) 1266
MspA1I CMGCKG 1 cut(s) 1339
MspI CCGG 3 cut(s) 561, 806, 1575
MspR9I CCNGG 6 cut(s) 180, 609, 806, 807, 1175, 1614
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 4 cut(s) 180, 609, 1175, 1614
MwoI GCNNNNNNNGC 2 cut(s) 378, 1378
NciI CCSGG 2 cut(s) 806, 807
NdeII GATC 3 cut(s) 208, 416, 526
NlaIII CATG 8 cut(s) 239, 269, 398, 411, 871, 1143, 1195, 1463
NlaIV GGNNCC 6 cut(s) 433, 901, 1132, 1179, 1189, 1518
NmuCI GTSAC 3 cut(s) 127, 140, 886
NspI RCATGY 1 cut(s) 398
PaeI GCATGC 1 cut(s) 398
PagI TCATGA 1 cut(s) 867
PasI CCCWGGG 1 cut(s) 608
PciSI GCTCTTC 4 cut(s) 18, 143, 634, 719
PctI GAATGC 1 cut(s) 526
PfeI GAWTC 3 cut(s) 271, 1153, 1464
PflMI CCANNNNNTGG 2 cut(s) 261, 1577
PinAI ACCGGT 1 cut(s) 560
PkrI GCNGC 2 cut(s) 697, 1201
PleI GAGTC 1 cut(s) 597
PpsI GAGTC 1 cut(s) 597
Ppu21I YACGTR 1 cut(s) 886
PpuMI RGGWCCY 1 cut(s) 1516
PshBI ATTAAT 1 cut(s) 819
Psp124BI GAGCTC 1 cut(s) 714
Psp5II RGGWCCY 1 cut(s) 1516
Psp6I CCWGG 4 cut(s) 178, 607, 1173, 1612
PspFI CCCAGC 3 cut(s) 662, 842, 1075
PspGI CCWGG 4 cut(s) 178, 607, 1173, 1612
PspN4I GGNNCC 6 cut(s) 433, 901, 1132, 1179, 1189, 1518
PspPI GGNCC 7 cut(s) 182, 302, 431, 569, 611, 1178, 1516
PspPPI RGGWCCY 1 cut(s) 1516
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 1 cut(s) 526
PvuII CAGCTG 1 cut(s) 1339
RsaI GTAC 3 cut(s) 353, 620, 1366
RsaNI GTAC 3 cut(s) 352, 619, 1365
RseI CAYNNNNRTG 3 cut(s) 224, 264, 1458
SacI GAGCTC 1 cut(s) 714
SapI GCTCTTC 4 cut(s) 18, 143, 634, 719
SaqAI TTAA 6 cut(s) 27, 222, 231, 437, 819, 1389
SatI GCNGC 2 cut(s) 696, 1200
Sau3AI GATC 3 cut(s) 208, 416, 526
Sau96I GGNCC 7 cut(s) 182, 302, 431, 569, 611, 1178, 1516
SchI GAGTC 1 cut(s) 597
ScrFI CCNGG 6 cut(s) 180, 609, 806, 807, 1175, 1614
SduI GDGCHC 2 cut(s) 714, 1336
SfaNI GCATC 2 cut(s) 574, 748
SinI GGWCC 5 cut(s) 302, 431, 569, 1178, 1516
SmaI CCCGGG 1 cut(s) 807
SmiMI CAYNNNNRTG 3 cut(s) 224, 264, 1458
SpeI ACTAGT 1 cut(s) 1421
SphI GCATGC 1 cut(s) 398
Sse9I AATT 7 cut(s) 173, 189, 927, 996, 1034, 1321, 1468
SsiI CCGC 1 cut(s) 873
SspMI CTAG 5 cut(s) 198, 896, 1395, 1422, 1601
SstI GAGCTC 1 cut(s) 714
StyD4I CCNGG 6 cut(s) 178, 607, 804, 805, 1173, 1612
TaaI ACNGT 5 cut(s) 163, 170, 344, 1364, 1561
TaiI ACGT 2 cut(s) 771, 888
TaqI TCGA 5 cut(s) 187, 274, 780, 1503, 1539
TasI AATT 7 cut(s) 173, 189, 927, 996, 1034, 1321, 1468
TatI WGTACW 2 cut(s) 351, 618
TfiI GAWTC 3 cut(s) 271, 1153, 1464
Tru1I TTAA 6 cut(s) 27, 222, 231, 437, 819, 1389
Tru9I TTAA 6 cut(s) 27, 222, 231, 437, 819, 1389
TscAI CASTG 4 cut(s) 266, 705, 1243, 1333
TseFI GTSAC 3 cut(s) 127, 140, 886
TseI GCWGC 2 cut(s) 695, 1199
Tsp45I GTSAC 3 cut(s) 127, 140, 886
TspDTI ATGAA 8 cut(s) 37, 134, 396, 856, 944, 986, 1442, 1448
TspGWI ACGGA 4 cut(s) 503, 615, 1040, 1305
TspMI CCCGGG 1 cut(s) 805
TspRI CASTG 4 cut(s) 266, 705, 1243, 1333
Van91I CCANNNNNTGG 2 cut(s) 261, 1577
VpaK11BI GGWCC 5 cut(s) 302, 431, 569, 1178, 1516
VspI ATTAAT 1 cut(s) 819
XapI RAATTY 2 cut(s) 927, 1034
XceI RCATGY 1 cut(s) 398
XcmI CCANNNNNNNNNTGG 2 cut(s) 1181, 1578
XmaI CCCGGG 1 cut(s) 805
XspI CTAG 5 cut(s) 198, 896, 1395, 1422, 1601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.