RchiOBHm_Chr5g0061891

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000610
Physical Location & Seq
Reverse (-)
67497257 .. 67500033
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33823

Sequence Viewer

Length: 1746 bp
ATGGGTGTCGCTCTTTTTTCGTTTAATAGGCTCTTTGTTCTCTCTTTCATCACATTGGGTCTCATTCCTCAGCTAGCTCCTGCAAGCATAACAAGGCACTACAAATTTGATGTACAACTGCAAAATGTGACAAGGCTGTGCCATACGAAAAGCATTGTAACCATAAACGGTCAATTTCCTGGGCCTCGAATTGTTGCTAGGGAGGGTGATCGCCTCCTCATTAAAGTTGTTAACCATGTCCGAAGCAACATTTCCATCCATTGGCATGGAATTCGACAGCTTCGAACTGGGTGGGCTGATGGTCCAGCATACATTACCCAATGCCCAATACAAACCGGCAACAGCTATGTGTACAACTTCACCATTGTAGGCCAGAGGGGCACTCTTTGGTGGCATGCTCACATTTCATGGCTACGATCAACCATCTATGGCCCCCTTATCATTCTTCCGGAGCGTGGTGTTCCTTATCCATTCACCAAACCCTATAAGGAAGTTCCCATCATCTTTGGAGAATGGTGGAATGCAGATCCTGAAGCAGTCATAAACCAGACCTTGCAAACTGGGGGAGGACCAAATGTTTCTGATGCTTACACCATCAATGGACTCCCAGGGCCATTGTATAACTGCTCTGCTAAAGATACTTTCAAGCTAAAGGTTCAAGCTGGGAAGACATACCTTCTGCGCCTAATCAATGCAGCGCTGAACGACGAGCTCTTCTTCAGCATTGCAAACCACACCCTCAAAGTGGTGGAAACTGATGCAGTTTACGTCAAACCCTTCGAGACCAACACCATTCTCATTGCCCCGGGACAAACCACAAATGTTCTTCTCAAAACAAAACCTAACTACCCAAACGCCACATTTTTCATGACTGCAAGGCCATACGTGACTGGTCTAGGCACCTTTGATAACTCCACTCTTGCTGGCATTTTAGAATACGAAACGACTACACTTCATTCAACCCTCCCAATGAACAAACTTCCACTCCACAAACCAATTCTCCCAGTTCTAAATGACACAACATTTGCAACAAAATTCTCCAATAATCTCCGTAGCTTGAACACTCCCCAGTTCCCTGCTAATGTTCCACAGAAAGTTGACAAGCATTTGTTCTTCACCATAGGGCTTGGAACCAACCCATGTAGCCAACAGAATCACAACAACCAGACTTGCCGAGGACCCAATGGAACCATGTTTGCTGCTTCAATCAACAACATTTCTTTCACTATGCCAAACACTGCCCTACTCCAATCACACTTCTTTGGCCAATCCAATGGGGTTTATTCCCTTAATTTTCCAATCAGTCCTTTAATTCCATTTAATTACACTGGAGCCCAGCTGAACAATACTAATGTGGGCAATGGTACTAAGCTAATGGTGCTTCCCTTTAACACTAGTGTGGAGTTGGTGTTGCAGGACACTAGTATTCTCGGTGCTGAAAGTCATCCCCTTCATCTTCATGGCTTTAATTTCTTTGTTGTTGGTCAAGGTTTTGGCAACTTCGACCAAAATAAGGACCCTAGGAAGTTCAATCTGATTGACCCTGTTGAGAGAAACACCGTGGGTGTGCCCTCCGGCGGGTGGGTTGCTATTCGGTTTCTAGCAGATAACCCGGGTGTATGGTTCATGCATTGCCACCTTGAGATCCATACTAGCTGGGGCCTGAAGATGGCTTGGGTAGTCTTAGATGGAAAGCTTTCCAATCAAAAGTTGCTTCCTCCGCCACTGGATCTGCCCAAATGTTGA

Protein Analysis

581

Amino Acids

64.63

Weight (kDa)

9.27

Isoelectric Point (pI)

28.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 38 - 151 1.7e-42 Multicopper oxidase
Cu-oxidase PF00394 164 - 314 1.2e-43 Multicopper oxidase
Cu-oxidase_2 PF07731 439 - 563 5e-41 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000610)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60020 AT5G60020 AT5G60020
fragaria_vesca FvH4_2g09062 FvH4_2g09062 FvH4_3g05920 FvH4_3g34100 FvH4_3g34310 FvH4_3g34340 FvH4_6g13390
malus_domestica MD00G1015000.v1.1 MD03G1106500.v1.1 MD03G1106600.v1.1 MD04G1131500.v1.1 MD11G1118900.v1.1 MD11G1119000.v1.1 MD12G1144600.v1.1
prunus_persica Prupe.6G089100_v2.0.a1 Prupe.6G089300_v2.0.a1 Prupe.6G258000_v2.0.a1
pyrus_communis pycom03g08590 pycom10g02940 pycom11g09990 pycom11g10060
rosa_chinensis RchiOBHm_Chr2g0154491 RchiOBHm_Chr3g0466271 RchiOBHm_Chr5g0061891 RchiOBHm_Chr5g0061911 RchiOBHm_Chr5g0061951 RchiOBHm_Chr5g0061961 RchiOBHm_Chr6g0264371
rosa_laevigata RLG00000024591 RLG00000035514 RLG00000035516 RLG00000035518 RLG00000035519
rosa_multiflora Rmu_sc0003480.1_g000021 Rmu_sc0011641.1_g000002 Rmu_sc0011641.1_g000009
rosa_roxburghii Rroxscaffold_1G00018790 Rroxscaffold_1G00018820 Rroxscaffold_1G00018850 Rroxscaffold_2G00122520 Rroxscaffold_2G00122540 Rroxscaffold_6G00414660
rosa_rugosa Rorug03G0080700 Rorug04G0180900 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345000 Rorug05G0345100 Rorug05G0345200 Rorug06G0011800
rosa_samantha Rh3DG148900 Rh5AG405700 Rh5AG406100 Rh5AG406200 Rh5BG419200 Rh5BG419400 Rh5CG443100 Rh5CG443300 Rh5CG443500 Rh5CG443600 Rh5DG432700 Rh5DG434900 Rh6BG128700 Rh6CG127600 Rh6DG114200 Rh6DG114300
rosa_wichuraiana Rw3G011980 Rw5G038100 Rw5G038140 Rw5G038160 Rw5G038200 Rw5G038210 Rw5G038250 Rw5G038270 Rw5G038290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 899
AccB7I CCANNNNNTGG 1 cut(s) 261
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 2 cut(s) 1578, 1721
AclWI GGATC 3 cut(s) 521, 1639, 1737
AcoI YGGCCR 1 cut(s) 1264
AcsI RAATTY 3 cut(s) 104, 270, 1034
AcuI CTGAAG 3 cut(s) 552, 703, 1685
AfaI GTAC 3 cut(s) 114, 353, 1366
AfeI AGCGCT 1 cut(s) 699
AfiI CCNNNNNNNGG 8 cut(s) 261, 455, 745, 1513, 1577, 1578, 1581, 1669
AgsI TTSAA 6 cut(s) 646, 659, 960, 1060, 1206, 1531
AhlI ACTAGT 2 cut(s) 1394, 1421
AjnI CCWGG 2 cut(s) 178, 607
AleI CACNNNNGTG 1 cut(s) 1397
Alw21I GWGCWC 1 cut(s) 714
Alw26I GTCTC 2 cut(s) 65, 776
AlwI GGATC 3 cut(s) 521, 1639, 1737
AlwNI CAGNNNCTG 1 cut(s) 530
Ama87I CYCGRG 2 cut(s) 805, 1612
Aor13HI TCCGGA 1 cut(s) 448
Aor51HI AGCGCT 1 cut(s) 699
AoxI GGCC 7 cut(s) 182, 370, 430, 611, 878, 1264, 1660
ApeKI GCWGC 2 cut(s) 695, 1199
ApoI RAATTY 3 cut(s) 104, 270, 1034
ArsI GACNNNNNNTTYG 2 cut(s) 1007, 1039
Asp700I GAANNNNTTC 1 cut(s) 1696
AspA2I CCTAGG 1 cut(s) 1520
AspLEI GCGC 2 cut(s) 684, 700
AspS9I GGNCC 8 cut(s) 182, 302, 431, 569, 611, 1178, 1516, 1660
AsuC2I CCSGG 4 cut(s) 806, 807, 1613, 1614
AsuHPI GGTGA 4 cut(s) 218, 352, 466, 1108
AsuII TTCGAA 1 cut(s) 283
AsuNHI GCTAGC 1 cut(s) 73
AvaI CYCGRG 2 cut(s) 805, 1612
AvaII GGWCC 4 cut(s) 302, 569, 1178, 1516
AvrII CCTAGG 1 cut(s) 1520
BaeGI GKGCMC 2 cut(s) 383, 1572
BalI TGGCCA 1 cut(s) 1266
BanI GGYRCC 1 cut(s) 899
BanII GRGCYC 2 cut(s) 714, 1336
BbsI GAAGAC 1 cut(s) 674
Bbv12I GWGCWC 1 cut(s) 714
BbvCI CCTCAGC 1 cut(s) 69
BbvI GCAGC 2 cut(s) 707, 1186
BccI CCATC 7 cut(s) 263, 293, 431, 506, 602, 1663, 1682
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 2 cut(s) 180, 609
BcnI CCSGG 4 cut(s) 806, 807, 1613, 1614
BcoDI GTCTC 2 cut(s) 65, 776
BcuI ACTAGT 2 cut(s) 1394, 1421
BfaI CTAG 8 cut(s) 74, 198, 896, 1395, 1422, 1521, 1601, 1653
BfoI RGCGCY 1 cut(s) 701
BglI GCCNNNNNGGC 1 cut(s) 378
BisI GCNGC 2 cut(s) 696, 1200
BlnI CCTAGG 1 cut(s) 1520
BlsI GCNGC 2 cut(s) 697, 1201
Bme1390I CCNGG 6 cut(s) 180, 609, 806, 807, 1613, 1614
Bme18I GGWCC 4 cut(s) 302, 569, 1178, 1516
BmeT110I CYCGRG 2 cut(s) 805, 1612
BmgT120I GGNCC 8 cut(s) 182, 302, 431, 569, 611, 1178, 1516, 1660
BmiI GGNNCC 8 cut(s) 433, 901, 1132, 1180, 1189, 1333, 1518, 1661
BmrFI CCNGG 6 cut(s) 180, 609, 806, 807, 1613, 1614
BmrI ACTGGG 4 cut(s) 297, 570, 998, 1063
BmsI GCATC 2 cut(s) 574, 748
BmtI GCTAGC 1 cut(s) 77
BmuI ACTGGG 4 cut(s) 297, 570, 998, 1063
BpiI GAAGAC 1 cut(s) 674
BplI GAGNNNNNCTC 2 cut(s) 367, 399
BpmI CTGGAG 1 cut(s) 1350
Bpu10I CCTNAGC 1 cut(s) 69
Bpu14I TTCGAA 1 cut(s) 283
BpuEI CTTGAG 1 cut(s) 1661
BpuMI CCSGG 4 cut(s) 806, 807, 1613, 1614
BsaAI YACGTR 1 cut(s) 886
BsaI GGTCTC 2 cut(s) 65, 776
BsaJI CCNNGG 9 cut(s) 179, 607, 608, 804, 805, 1174, 1520, 1560, 1612
BsaWI WCCGGW 1 cut(s) 448
BsaXI ACNNNNNCTCC 6 cut(s) 969, 984, 999, 1014, 1394, 1424
Bsc4I CCNNNNNNNGG 8 cut(s) 261, 455, 745, 1513, 1577, 1578, 1581, 1669
Bse118I RCCGGY 1 cut(s) 335
Bse1I ACTGG 7 cut(s) 292, 565, 895, 1004, 1069, 1333, 1731
Bse3DI GCAATG 4 cut(s) 723, 798, 1366, 1630
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 2 cut(s) 180, 609
BseDI CCNNGG 9 cut(s) 179, 607, 608, 804, 805, 1174, 1520, 1560, 1612
BseGI GGATG 2 cut(s) 255, 1444
BseLI CCNNNNNNNGG 8 cut(s) 261, 455, 745, 1513, 1577, 1578, 1581, 1669
BseMI GCAATG 4 cut(s) 723, 798, 1366, 1630
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 7 cut(s) 292, 565, 895, 1004, 1069, 1333, 1731
BseRI GAGGAG 1 cut(s) 206
BseSI GKGCMC 2 cut(s) 383, 1572
BseXI GCAGC 2 cut(s) 707, 1186
BseYI CCCAGC 3 cut(s) 662, 1335, 1656
BshFI GGCC 7 cut(s) 184, 372, 432, 613, 880, 1266, 1662
BshNI GGYRCC 1 cut(s) 899
BsiHKAI GWGCWC 1 cut(s) 714
BsiHKCI CYCGRG 2 cut(s) 805, 1612
BsiSI CCGG 5 cut(s) 336, 449, 806, 1575, 1613
BslFI GGGAC 1 cut(s) 822
BslI CCNNNNNNNGG 8 cut(s) 261, 455, 745, 1513, 1577, 1578, 1581, 1669
BsmAI GTCTC 2 cut(s) 65, 776
BsmFI GGGAC 1 cut(s) 822
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 7 cut(s) 184, 372, 432, 613, 880, 1266, 1662
Bso31I GGTCTC 2 cut(s) 65, 776
BsoBI CYCGRG 2 cut(s) 805, 1612
Bsp119I TTCGAA 1 cut(s) 283
Bsp1286I GDGCHC 4 cut(s) 383, 714, 1336, 1572
Bsp13I TCCGGA 1 cut(s) 448
Bsp1407I TGTACA 2 cut(s) 112, 351
Bsp143I GATC 5 cut(s) 208, 416, 526, 1644, 1729
BspACI CCGC 2 cut(s) 1578, 1721
BspANI GGCC 7 cut(s) 184, 372, 432, 613, 880, 1266, 1662
BspCNI CTCAG 1 cut(s) 82
BspEI TCCGGA 1 cut(s) 448
BspHI TCATGA 1 cut(s) 867
BspLI GGNNCC 8 cut(s) 433, 901, 1132, 1180, 1189, 1333, 1518, 1661
BspOI GCTAGC 1 cut(s) 77
BspPI GGATC 3 cut(s) 521, 1639, 1737
BspQI GCTCTTC 1 cut(s) 719
BspT104I TTCGAA 1 cut(s) 283
BspT107I GGYRCC 1 cut(s) 899
BspTNI GGTCTC 2 cut(s) 65, 776
BsrDI GCAATG 4 cut(s) 723, 798, 1366, 1630
BsrFI RCCGGY 1 cut(s) 335
BsrGI TGTACA 2 cut(s) 112, 351
BsrI ACTGG 7 cut(s) 292, 565, 895, 1004, 1069, 1333, 1731
BssAI RCCGGY 1 cut(s) 335
BssECI CCNNGG 9 cut(s) 179, 607, 608, 804, 805, 1174, 1520, 1560, 1612
BssMI GATC 5 cut(s) 208, 416, 526, 1644, 1729
BssT1I CCWWGG 1 cut(s) 1520
Bst2UI CCWGG 2 cut(s) 180, 609
Bst4CI ACNGT 2 cut(s) 170, 1561
Bst6I CTCTTC 1 cut(s) 719
BstAUI TGTACA 2 cut(s) 112, 351
BstBAI YACGTR 1 cut(s) 886
BstBI TTCGAA 1 cut(s) 283
BstC8I GCNNGC 4 cut(s) 75, 85, 396, 925
BstDEI CTNAG 3 cut(s) 69, 1368, 1684
BstDSI CCRYGG 1 cut(s) 1560
BstF5I GGATG 2 cut(s) 255, 1444
BstH2I RGCGCY 1 cut(s) 701
BstHHI GCGC 2 cut(s) 684, 700
BstKTI GATC 5 cut(s) 211, 419, 529, 1647, 1732
BstMAI GTCTC 2 cut(s) 65, 776
BstMBI GATC 5 cut(s) 208, 416, 526, 1644, 1729
BstMWI GCNNNNNNNGC 3 cut(s) 378, 1378, 1720
BstNI CCWGG 2 cut(s) 180, 609
BstNSI RCATGY 1 cut(s) 398
BstSCI CCNGG 6 cut(s) 178, 607, 804, 805, 1611, 1612
BstSLI GKGCMC 2 cut(s) 383, 1572
BstV1I GCAGC 2 cut(s) 707, 1186
BstV2I GAAGAC 1 cut(s) 674
BstX2I RGATCY 3 cut(s) 526, 1644, 1729
BstXI CCANNNNNNTGG 2 cut(s) 266, 1274
BstYI RGATCY 3 cut(s) 526, 1644, 1729
BsuRI GGCC 7 cut(s) 184, 372, 432, 613, 880, 1266, 1662
BtgI CCRYGG 1 cut(s) 1560
BtsCI GGATG 2 cut(s) 255, 1444
BtsI GCAGTG 1 cut(s) 1236
BtsIMutI CAGTG 3 cut(s) 1236, 1326, 1724
Cac8I GCNNGC 4 cut(s) 75, 85, 396, 925
CaiI CAGNNNCTG 1 cut(s) 530
CciI TCATGA 1 cut(s) 867
CfoI GCGC 2 cut(s) 684, 700
Cfr10I RCCGGY 1 cut(s) 335
Cfr13I GGNCC 8 cut(s) 182, 302, 431, 569, 611, 1178, 1516, 1660
Cfr9I CCCGGG 2 cut(s) 805, 1612
Csp6I GTAC 3 cut(s) 113, 352, 1365
CviAII CATG 9 cut(s) 236, 266, 395, 408, 868, 1140, 1192, 1460, 1627
CviQI GTAC 3 cut(s) 113, 352, 1365
DdeI CTNAG 3 cut(s) 69, 1368, 1684
DpnI GATC 5 cut(s) 210, 418, 528, 1646, 1731
DpnII GATC 5 cut(s) 208, 416, 526, 1644, 1729
EaeI YGGCCR 1 cut(s) 1264
Eam1104I CTCTTC 1 cut(s) 719
EarI CTCTTC 1 cut(s) 719
EciI GGCGGA 1 cut(s) 1710
Ecl136II GAGCTC 1 cut(s) 712
Eco130I CCWWGG 1 cut(s) 1520
Eco24I GRGCYC 2 cut(s) 714, 1336
Eco31I GGTCTC 2 cut(s) 65, 776
Eco47I GGWCC 4 cut(s) 302, 569, 1178, 1516
Eco47III AGCGCT 1 cut(s) 699
Eco53kI GAGCTC 1 cut(s) 712
Eco57I CTGAAG 3 cut(s) 552, 703, 1685
Eco88I CYCGRG 2 cut(s) 805, 1612
EcoICRI GAGCTC 1 cut(s) 712
EcoO109I RGGNCCY 3 cut(s) 1178, 1516, 1660
EcoRI GAATTC 1 cut(s) 270
EcoRII CCWGG 2 cut(s) 178, 607
EcoT14I CCWWGG 1 cut(s) 1520
EcoT22I ATGCAT 1 cut(s) 1632
EcoT38I GRGCYC 2 cut(s) 714, 1336
ErhI CCWWGG 1 cut(s) 1520
FaeI CATG 9 cut(s) 239, 269, 398, 411, 871, 1143, 1195, 1463, 1630
FaqI GGGAC 1 cut(s) 822
FatI CATG 9 cut(s) 235, 265, 394, 407, 867, 1139, 1191, 1459, 1626
FauI CCCGC 1 cut(s) 1571
Fnu4HI GCNGC 2 cut(s) 696, 1200
FokI GGATG 2 cut(s) 242, 1431
FriOI GRGCYC 2 cut(s) 714, 1336
Fsp4HI GCNGC 2 cut(s) 696, 1200
FspBI CTAG 8 cut(s) 74, 198, 896, 1395, 1422, 1521, 1601, 1653
GlaI GCGC 2 cut(s) 683, 699
GluI GCNGC 2 cut(s) 696, 1200
GsaI CCCAGC 3 cut(s) 666, 1339, 1660
GsuI CTGGAG 1 cut(s) 1350
HaeII RGCGCY 1 cut(s) 701
HaeIII GGCC 7 cut(s) 184, 372, 432, 613, 880, 1266, 1662
HapII CCGG 5 cut(s) 336, 449, 806, 1575, 1613
HhaI GCGC 2 cut(s) 684, 700
Hin1II CATG 9 cut(s) 239, 269, 398, 411, 871, 1143, 1195, 1463, 1630
Hin6I GCGC 2 cut(s) 682, 698
HinP1I GCGC 2 cut(s) 682, 698
HincII GTYRAC 2 cut(s) 232, 1099
HindII GTYRAC 2 cut(s) 232, 1099
HindIII AAGCTT 1 cut(s) 1694
HinfI GANTC 2 cut(s) 603, 1153
HpaI GTTAAC 1 cut(s) 232
HpaII CCGG 5 cut(s) 336, 449, 806, 1575, 1613
HphI GGTGA 4 cut(s) 218, 352, 466, 1108
Hpy166II GTNNAC 4 cut(s) 232, 352, 766, 1099
Hpy188I TCNGA 3 cut(s) 242, 583, 1536
Hpy188III TCNNGA 4 cut(s) 449, 530, 781, 868
Hpy8I GTNNAC 4 cut(s) 232, 352, 766, 1099
Hpy99I CGWCG 1 cut(s) 710
HpyAV CCTTC 3 cut(s) 686, 787, 1460
HpyCH4III ACNGT 2 cut(s) 170, 1561
HpyCH4IV ACGT 2 cut(s) 768, 885
HpyF10VI GCNNNNNNNGC 3 cut(s) 378, 1378, 1720
HpyF3I CTNAG 3 cut(s) 69, 1368, 1684
HpySE526I ACGT 2 cut(s) 768, 885
Hsp92II CATG 9 cut(s) 239, 269, 398, 411, 871, 1143, 1195, 1463, 1630
HspAI GCGC 2 cut(s) 682, 698
Kpn2I TCCGGA 1 cut(s) 448
KspAI GTTAAC 1 cut(s) 232
Kzo9I GATC 5 cut(s) 208, 416, 526, 1644, 1729
LguI GCTCTTC 1 cut(s) 719
LmnI GCTCC 3 cut(s) 82, 451, 1331
Lsp1109I GCAGC 2 cut(s) 707, 1186
LweI GCATC 2 cut(s) 574, 748
MaeI CTAG 8 cut(s) 74, 198, 896, 1395, 1422, 1521, 1601, 1653
MaeII ACGT 2 cut(s) 768, 885
MaeIII GTNAC 3 cut(s) 127, 157, 886
MalI GATC 5 cut(s) 210, 418, 528, 1646, 1731
MboI GATC 5 cut(s) 208, 416, 526, 1644, 1729
MboII GAAGA 8 cut(s) 437, 679, 706, 709, 818, 1105, 1448, 1678
MflI RGATCY 3 cut(s) 526, 1644, 1729
MhlI GDGCHC 4 cut(s) 383, 714, 1336, 1572
MlsI TGGCCA 1 cut(s) 1266
MluNI TGGCCA 1 cut(s) 1266
MlyI GAGTC 1 cut(s) 597
Mox20I TGGCCA 1 cut(s) 1266
Mph1103I ATGCAT 1 cut(s) 1632
MroI TCCGGA 1 cut(s) 448
MroXI GAANNNNTTC 1 cut(s) 1696
MscI TGGCCA 1 cut(s) 1266
MseI TTAA 8 cut(s) 24, 222, 231, 1290, 1310, 1320, 1389, 1467
MslI CAYNNNNRTG 3 cut(s) 264, 1397, 1458
Msp20I TGGCCA 1 cut(s) 1266
MspA1I CMGCKG 1 cut(s) 1339
MspI CCGG 5 cut(s) 336, 449, 806, 1575, 1613
MspR9I CCNGG 6 cut(s) 180, 609, 806, 807, 1613, 1614
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 2 cut(s) 180, 609
MwoI GCNNNNNNNGC 3 cut(s) 378, 1378, 1720
NciI CCSGG 4 cut(s) 806, 807, 1613, 1614
NdeII GATC 5 cut(s) 208, 416, 526, 1644, 1729
NheI GCTAGC 1 cut(s) 73
NlaIII CATG 9 cut(s) 239, 269, 398, 411, 871, 1143, 1195, 1463, 1630
NlaIV GGNNCC 8 cut(s) 433, 901, 1132, 1180, 1189, 1333, 1518, 1661
NmeAIII GCCGAG 1 cut(s) 1199
NmuCI GTSAC 2 cut(s) 127, 886
NsiI ATGCAT 1 cut(s) 1632
NspI RCATGY 1 cut(s) 398
NspV TTCGAA 1 cut(s) 283
OliI CACNNNNGTG 1 cut(s) 1397
PaeI GCATGC 1 cut(s) 398
PagI TCATGA 1 cut(s) 867
PasI CCCWGGG 1 cut(s) 608
PciSI GCTCTTC 1 cut(s) 719
PcsI WCGNNNNNNNCGW 1 cut(s) 280
PctI GAATGC 1 cut(s) 526
PdmI GAANNNNTTC 1 cut(s) 1696
PfeI GAWTC 1 cut(s) 1153
PflMI CCANNNNNTGG 1 cut(s) 261
PkrI GCNGC 2 cut(s) 697, 1201
PleI GAGTC 1 cut(s) 597
PpsI GAGTC 1 cut(s) 597
Ppu21I YACGTR 1 cut(s) 886
PpuMI RGGWCCY 2 cut(s) 1178, 1516
Psp124BI GAGCTC 1 cut(s) 714
Psp5II RGGWCCY 2 cut(s) 1178, 1516
Psp6I CCWGG 2 cut(s) 178, 607
PspFI CCCAGC 3 cut(s) 662, 1335, 1656
PspGI CCWGG 2 cut(s) 178, 607
PspN4I GGNNCC 8 cut(s) 433, 901, 1132, 1180, 1189, 1333, 1518, 1661
PspPI GGNCC 8 cut(s) 182, 302, 431, 569, 611, 1178, 1516, 1660
PspPPI RGGWCCY 2 cut(s) 1178, 1516
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 3 cut(s) 526, 1644, 1729
PvuII CAGCTG 1 cut(s) 1339
RsaI GTAC 3 cut(s) 114, 353, 1366
RsaNI GTAC 3 cut(s) 113, 352, 1365
RseI CAYNNNNRTG 3 cut(s) 264, 1397, 1458
SacI GAGCTC 1 cut(s) 714
SapI GCTCTTC 1 cut(s) 719
SaqAI TTAA 8 cut(s) 24, 222, 231, 1290, 1310, 1320, 1389, 1467
SatI GCNGC 2 cut(s) 696, 1200
Sau3AI GATC 5 cut(s) 208, 416, 526, 1644, 1729
Sau96I GGNCC 8 cut(s) 182, 302, 431, 569, 611, 1178, 1516, 1660
SchI GAGTC 1 cut(s) 597
ScrFI CCNGG 6 cut(s) 180, 609, 806, 807, 1613, 1614
SduI GDGCHC 4 cut(s) 383, 714, 1336, 1572
SfaNI GCATC 2 cut(s) 574, 748
SfuI TTCGAA 1 cut(s) 283
SinI GGWCC 4 cut(s) 302, 569, 1178, 1516
SmaI CCCGGG 2 cut(s) 807, 1614
SmiMI CAYNNNNRTG 3 cut(s) 264, 1397, 1458
SmlI CTYRAG 1 cut(s) 1640
SmoI CTYRAG 1 cut(s) 1640
SpeI ACTAGT 2 cut(s) 1394, 1421
SphI GCATGC 1 cut(s) 398
SsiI CCGC 2 cut(s) 1578, 1721
SspMI CTAG 8 cut(s) 74, 198, 896, 1395, 1422, 1521, 1601, 1653
SstI GAGCTC 1 cut(s) 714
StyD4I CCNGG 6 cut(s) 178, 607, 804, 805, 1611, 1612
StyI CCWWGG 1 cut(s) 1520
TaaI ACNGT 2 cut(s) 170, 1561
TaiI ACGT 2 cut(s) 771, 888
TaqI TCGA 5 cut(s) 187, 274, 283, 780, 1503
TatI WGTACW 2 cut(s) 112, 351
TfiI GAWTC 1 cut(s) 1153
Tru1I TTAA 8 cut(s) 24, 222, 231, 1290, 1310, 1320, 1389, 1467
Tru9I TTAA 8 cut(s) 24, 222, 231, 1290, 1310, 1320, 1389, 1467
TscAI CASTG 3 cut(s) 1243, 1333, 1731
TseFI GTSAC 2 cut(s) 127, 886
TseI GCWGC 2 cut(s) 695, 1199
Tsp45I GTSAC 2 cut(s) 127, 886
TspDTI ATGAA 8 cut(s) 37, 396, 856, 944, 986, 1442, 1448, 1615
TspGWI ACGGA 1 cut(s) 1040
TspMI CCCGGG 2 cut(s) 805, 1612
TspRI CASTG 3 cut(s) 1243, 1333, 1731
Van91I CCANNNNNTGG 1 cut(s) 261
VpaK11BI GGWCC 4 cut(s) 302, 569, 1178, 1516
XapI RAATTY 3 cut(s) 104, 270, 1034
XceI RCATGY 1 cut(s) 398
XmaI CCCGGG 2 cut(s) 805, 1612
XmaJI CCTAGG 1 cut(s) 1520
XmnI GAANNNNTTC 1 cut(s) 1696
XspI CTAG 8 cut(s) 74, 198, 896, 1395, 1422, 1521, 1601, 1653
Zsp2I ATGCAT 1 cut(s) 1632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.