MD12G1144500.v1.1

Belongs to the DHHC palmitoyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007849
Physical Location & Seq
Reverse (-)
22369645 .. 22374777
5133 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1144500.v1.1.491

Sequence Viewer

Length: 855 bp
ATGACGCGAAGCCTCGGCTTCTCTCTACCCGTGACCGTCGTCGTTTTGGCAATCGCATACATTTACTTCTCCACAGTCTTCATTTTCATAGACCGCTGGTTCAGTCTCATGTCGTCGCCAGGTTTGATGAACGCCGTCGTTTTCACCGGCGTGGCCGTCATGTGCGTATTCAATTACTTCGTCTCCGTCTTCAGGGATCCGGGTAGGGTTCCCTCCACGTACATGCCCGACGTCGAAGATTCGGGTAACCCTATCCACGAGATCAAGCGCAAGGGAGGGGATTTAAGATATTGCCAAAAGTGTTCTCACTATAAGCCAGCTCGTGCACATCATTGTCGTGTATGCAAACGATGTGTATTGCGAATGGATCATCATTGCATATGGATTAATAATTGCGTCGGCCATGCAAACTATAAGGTCTTCTTCATCTTCGTTGTTTATGCTGTAATAGCATGCCTCTATTCCCTGGTTTTGCTCTTGGGTAGCCTAACCTATGATCCCGAAAAAGATGACCAGGAAACTGGTGACTCTTTTCGAACTGCATATGTCATTTCTGGGCTGTTGCTAGTTCCCTTATGTGTGGCACTGAGTGTTCTTTTAGGTTGGCATATCTACCTCATTTTGCAAAACAAGACCACAATTGAGTACTATGAAGGAGTGAGAGCTATGTGGCTTGCAGAGAAAGGAGGGCAAATCTATTCCCATCCATATGATCTCGGTGCCTATGAAAATTTAACAACGGTAATGGGTCCAAATATCTGCTGTTGGGCACTCCCCACATCGGGACACATAGGATCGGGACTTCGCTTTCGTACAGCTTATGATTACCCTTCCGGAGCATCAGCGTTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DHHC

Protein Analysis

285

Amino Acids

31.96

Weight (kDa)

7.94

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHC PF01529 94 - 218 4.9e-41 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 234
AccB1I GGYRCC 1 cut(s) 719
AccII CGCG 1 cut(s) 7
AccIII TCCGGA 1 cut(s) 833
AciI CCGC 1 cut(s) 94
AclWI GGATC 5 cut(s) 191, 204, 375, 491, 802
AcoI YGGCCR 2 cut(s) 153, 400
AcsI RAATTY 1 cut(s) 730
AcuI CTGAAG 1 cut(s) 175
AcyI GRCGYC 1 cut(s) 231
AdeI CACNNNGTG 1 cut(s) 590
AfaI GTAC 3 cut(s) 221, 647, 814
AfiI CCNNNNNNNGG 3 cut(s) 192, 781, 782
AgsI TTSAA 1 cut(s) 172
AjnI CCWGG 3 cut(s) 118, 465, 513
AleI CACNNNNGTG 1 cut(s) 149
AluBI AGCT 3 cut(s) 320, 665, 818
AluI AGCT 3 cut(s) 320, 665, 818
Alw21I GWGCWC 1 cut(s) 328
Alw26I GTCTC 2 cut(s) 110, 187
Alw44I GTGCAC 1 cut(s) 324
AlwI GGATC 5 cut(s) 191, 204, 375, 491, 802
Aor13HI TCCGGA 1 cut(s) 833
AoxI GGCC 2 cut(s) 153, 400
ApaLI GTGCAC 1 cut(s) 324
ApoI RAATTY 1 cut(s) 730
ArsI GACNNNNNNTTYG 2 cut(s) 792, 824
AseI ATTAAT 1 cut(s) 387
AspLEI GCGC 1 cut(s) 270
AspS9I GGNCC 1 cut(s) 749
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 2 cut(s) 136, 536
AsuII TTCGAA 1 cut(s) 535
AvaII GGWCC 1 cut(s) 749
BaeGI GKGCMC 2 cut(s) 328, 772
BamHI GGATCC 1 cut(s) 196
BanI GGYRCC 1 cut(s) 719
BauI CACGAG 2 cut(s) 257, 321
BbsI GAAGAC 3 cut(s) 70, 181, 412
Bbv12I GWGCWC 1 cut(s) 328
BccI CCATC 1 cut(s) 711
BceAI ACGGC 2 cut(s) 119, 140
BciT130I CCWGG 3 cut(s) 120, 467, 515
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 2 cut(s) 110, 187
BfaI CTAG 1 cut(s) 566
BmcAI AGTACT 1 cut(s) 647
Bme1390I CCNGG 4 cut(s) 120, 201, 467, 515
Bme18I GGWCC 1 cut(s) 749
BmgT120I GGNCC 1 cut(s) 749
BmiI GGNNCC 4 cut(s) 198, 210, 721, 750
BmrFI CCNGG 4 cut(s) 120, 201, 467, 515
BmsI GCATC 1 cut(s) 848
BoxI GACNNNNGTC 1 cut(s) 38
BpiI GAAGAC 3 cut(s) 70, 181, 412
Bpu14I TTCGAA 1 cut(s) 535
BpuMI CCSGG 1 cut(s) 201
BsaAI YACGTR 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 13, 465
BsaWI WCCGGW 1 cut(s) 833
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 781, 782
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 1 cut(s) 526
Bse3DI GCAATG 1 cut(s) 373
BseAI TCCGGA 1 cut(s) 833
BseBI CCWGG 3 cut(s) 120, 467, 515
BseDI CCNNGG 2 cut(s) 13, 465
BseGI GGATG 1 cut(s) 703
BseLI CCNNNNNNNGG 3 cut(s) 192, 781, 782
BseMI GCAATG 1 cut(s) 373
BseMII CTCAG 1 cut(s) 578
BseNI ACTGG 1 cut(s) 526
BseSI GKGCMC 2 cut(s) 328, 772
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 2 cut(s) 155, 402
BshNI GGYRCC 1 cut(s) 719
BsiHKAI GWGCWC 1 cut(s) 328
BsiSI CCGG 3 cut(s) 147, 200, 834
BslFI GGGAC 2 cut(s) 798, 813
BslI CCNNNNNNNGG 3 cut(s) 192, 781, 782
BsmAI GTCTC 2 cut(s) 110, 187
BsmBI CGTCTC 1 cut(s) 187
BsmFI GGGAC 2 cut(s) 798, 813
BsnI GGCC 2 cut(s) 155, 402
Bsp119I TTCGAA 1 cut(s) 535
Bsp1286I GDGCHC 2 cut(s) 328, 772
Bsp13I TCCGGA 1 cut(s) 833
Bsp143I GATC 6 cut(s) 196, 261, 367, 496, 712, 794
BspACI CCGC 1 cut(s) 94
BspANI GGCC 2 cut(s) 155, 402
BspCNI CTCAG 1 cut(s) 579
BspEI TCCGGA 1 cut(s) 833
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 4 cut(s) 198, 210, 721, 750
BspPI GGATC 5 cut(s) 191, 204, 375, 491, 802
BspT104I TTCGAA 1 cut(s) 535
BspT107I GGYRCC 1 cut(s) 719
BsrDI GCAATG 1 cut(s) 373
BsrFI RCCGGY 1 cut(s) 146
BsrI ACTGG 1 cut(s) 526
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 2 cut(s) 13, 465
BssMI GATC 6 cut(s) 196, 261, 367, 496, 712, 794
BssNI GRCGYC 1 cut(s) 231
BssSI CACGAG 2 cut(s) 257, 321
Bst2BI CACGAG 2 cut(s) 257, 321
Bst2UI CCWGG 3 cut(s) 120, 467, 515
Bst4CI ACNGT 3 cut(s) 37, 76, 742
BstACI GRCGYC 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 219
BstBI TTCGAA 1 cut(s) 535
BstC8I GCNNGC 3 cut(s) 318, 454, 675
BstDEI CTNAG 1 cut(s) 587
BstEII GGTNACC 1 cut(s) 245
BstF5I GGATG 1 cut(s) 703
BstFNI CGCG 1 cut(s) 7
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 6 cut(s) 199, 264, 370, 499, 715, 797
BstMAI GTCTC 2 cut(s) 110, 187
BstMBI GATC 6 cut(s) 196, 261, 367, 496, 712, 794
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstNI CCWGG 3 cut(s) 120, 467, 515
BstNSI RCATGY 2 cut(s) 226, 456
BstPAI GACNNNNGTC 1 cut(s) 38
BstPI GGTNACC 1 cut(s) 245
BstSCI CCNGG 4 cut(s) 118, 199, 465, 513
BstSLI GKGCMC 2 cut(s) 328, 772
BstUI CGCG 1 cut(s) 7
BstV2I GAAGAC 3 cut(s) 70, 181, 412
BstX2I RGATCY 1 cut(s) 196
BstXI CCANNNNNNTGG 1 cut(s) 521
BstYI RGATCY 1 cut(s) 196
BsuRI GGCC 2 cut(s) 155, 402
BtsCI GGATG 1 cut(s) 703
BtsIMutI CAGTG 1 cut(s) 584
Cac8I GCNNGC 3 cut(s) 318, 454, 675
CfoI GCGC 1 cut(s) 270
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 1 cut(s) 749
CseI GACGC 2 cut(s) 13, 385
Csp6I GTAC 3 cut(s) 220, 646, 813
CviAII CATG 5 cut(s) 109, 160, 223, 404, 453
CviQI GTAC 3 cut(s) 220, 646, 813
DdeI CTNAG 1 cut(s) 587
DpnI GATC 6 cut(s) 198, 263, 369, 498, 714, 796
DpnII GATC 6 cut(s) 196, 261, 367, 496, 712, 794
DraIII CACNNNGTG 1 cut(s) 590
EaeI YGGCCR 2 cut(s) 153, 400
Eco47I GGWCC 1 cut(s) 749
Eco57I CTGAAG 1 cut(s) 175
Eco91I GGTNACC 1 cut(s) 245
EcoO65I GGTNACC 1 cut(s) 245
EcoRII CCWGG 3 cut(s) 118, 465, 513
Esp3I CGTCTC 1 cut(s) 187
FaeI CATG 5 cut(s) 112, 163, 226, 407, 456
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FaqI GGGAC 2 cut(s) 798, 813
FatI CATG 5 cut(s) 108, 159, 222, 403, 452
FauNDI CATATG 3 cut(s) 380, 544, 709
FokI GGATG 1 cut(s) 690
FspBI CTAG 1 cut(s) 566
GlaI GCGC 1 cut(s) 269
HaeIII GGCC 2 cut(s) 155, 402
HapII CCGG 3 cut(s) 147, 200, 834
HgaI GACGC 2 cut(s) 13, 385
HhaI GCGC 1 cut(s) 270
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 5 cut(s) 112, 163, 226, 407, 456
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HinfI GANTC 2 cut(s) 239, 527
HpaII CCGG 3 cut(s) 147, 200, 834
HphI GGTGA 2 cut(s) 136, 536
Hpy166II GTNNAC 1 cut(s) 326
Hpy188III TCNNGA 4 cut(s) 500, 783, 798, 834
Hpy8I GTNNAC 1 cut(s) 326
Hpy99I CGWCG 7 cut(s) 41, 44, 118, 140, 233, 236, 401
HpyAV CCTTC 2 cut(s) 647, 840
HpyCH4III ACNGT 3 cut(s) 37, 76, 742
HpyCH4IV ACGT 2 cut(s) 218, 231
HpyCH4V TGCA 7 cut(s) 326, 345, 378, 407, 542, 625, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 1 cut(s) 587
HpySE526I ACGT 2 cut(s) 218, 231
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 5 cut(s) 112, 163, 226, 407, 456
HspAI GCGC 1 cut(s) 268
Kpn2I TCCGGA 1 cut(s) 833
Kzo9I GATC 6 cut(s) 196, 261, 367, 496, 712, 794
LmnI GCTCC 1 cut(s) 836
LweI GCATC 1 cut(s) 848
MaeI CTAG 1 cut(s) 566
MaeII ACGT 2 cut(s) 218, 231
MaeIII GTNAC 3 cut(s) 31, 245, 524
MalI GATC 6 cut(s) 198, 263, 369, 498, 714, 796
MboI GATC 6 cut(s) 196, 261, 367, 496, 712, 794
MboII GAAGA 6 cut(s) 70, 181, 248, 412, 415, 421
MfeI CAATTG 1 cut(s) 639
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 2 cut(s) 328, 772
MluCI AATT 4 cut(s) 172, 391, 639, 730
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 1 cut(s) 828
MnlI CCTC 6 cut(s) 23, 223, 269, 467, 626, 680
MroI TCCGGA 1 cut(s) 833
MseI TTAA 3 cut(s) 284, 387, 734
MslI CAYNNNNRTG 4 cut(s) 149, 221, 336, 708
MspA1I CMGCKG 1 cut(s) 96
MspI CCGG 3 cut(s) 147, 200, 834
MspR9I CCNGG 4 cut(s) 120, 201, 467, 515
MunI CAATTG 1 cut(s) 639
MvaI CCWGG 3 cut(s) 120, 467, 515
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 1 cut(s) 449
NciI CCSGG 1 cut(s) 201
NdeI CATATG 3 cut(s) 380, 544, 709
NdeII GATC 6 cut(s) 196, 261, 367, 496, 712, 794
NlaIII CATG 5 cut(s) 112, 163, 226, 407, 456
NlaIV GGNNCC 4 cut(s) 198, 210, 721, 750
NmuCI GTSAC 2 cut(s) 31, 524
NspI RCATGY 2 cut(s) 226, 456
NspV TTCGAA 1 cut(s) 535
OliI CACNNNNGTG 1 cut(s) 149
PaeI GCATGC 1 cut(s) 456
PfeI GAWTC 1 cut(s) 239
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
Ppu21I YACGTR 1 cut(s) 219
PshAI GACNNNNGTC 1 cut(s) 38
PshBI ATTAAT 1 cut(s) 387
Psp6I CCWGG 3 cut(s) 118, 465, 513
PspEI GGTNACC 1 cut(s) 245
PspGI CCWGG 3 cut(s) 118, 465, 513
PspN4I GGNNCC 4 cut(s) 198, 210, 721, 750
PspPI GGNCC 1 cut(s) 749
PsuI RGATCY 1 cut(s) 196
RsaI GTAC 3 cut(s) 221, 647, 814
RsaNI GTAC 3 cut(s) 220, 646, 813
RseI CAYNNNNRTG 4 cut(s) 149, 221, 336, 708
SaqAI TTAA 3 cut(s) 284, 387, 734
Sau3AI GATC 6 cut(s) 196, 261, 367, 496, 712, 794
Sau96I GGNCC 1 cut(s) 749
ScaI AGTACT 1 cut(s) 647
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 4 cut(s) 120, 201, 467, 515
SduI GDGCHC 2 cut(s) 328, 772
SfaNI GCATC 1 cut(s) 848
SfuI TTCGAA 1 cut(s) 535
SgrAI CRCCGGYG 1 cut(s) 146
SinI GGWCC 1 cut(s) 749
SmiMI CAYNNNNRTG 4 cut(s) 149, 221, 336, 708
SphI GCATGC 1 cut(s) 456
Sse9I AATT 4 cut(s) 172, 391, 639, 730
SsiI CCGC 1 cut(s) 94
SspMI CTAG 1 cut(s) 566
StyD4I CCNGG 4 cut(s) 118, 199, 465, 513
TaaI ACNGT 3 cut(s) 37, 76, 742
TaiI ACGT 2 cut(s) 221, 234
TaqI TCGA 2 cut(s) 234, 535
TasI AATT 4 cut(s) 172, 391, 639, 730
TatI WGTACW 1 cut(s) 645
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 3 cut(s) 284, 387, 734
Tru9I TTAA 3 cut(s) 284, 387, 734
TscAI CASTG 1 cut(s) 591
TseFI GTSAC 2 cut(s) 31, 524
Tsp45I GTSAC 2 cut(s) 31, 524
TspDTI ATGAA 6 cut(s) 70, 76, 143, 415, 666, 741
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 1 cut(s) 591
VneI GTGCAC 1 cut(s) 324
VpaK11BI GGWCC 1 cut(s) 749
VspI ATTAAT 1 cut(s) 387
XapI RAATTY 1 cut(s) 730
XceI RCATGY 2 cut(s) 226, 456
XspI CTAG 1 cut(s) 566
ZraI GACGTC 1 cut(s) 232
ZrmI AGTACT 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.