MD04G1135400.v1.1

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007631
Physical Location & Seq
Forward (+)
22242112 .. 22244002
1891 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1135400.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGACTGCAATGAAGGAGGAAGAGGAGGCTCGACGAAAACGGAGGAGCGGGGAGAAGCTCTGGTGTATATGTGGGGATATCTTCCCGGAGTATCGTCGGAGAAGTCTCCGACACTGTCTCTGACGCCGGTGCAGTTCCCGAATCGGATGGACGGTGGGGATTCGTGGAAGGACGTCTGCGGCGGCGGTTGTGGATTCGCCATGGCCATCTCAGAGTGTGGCAAGTTAATGACATGGGGGTCGACCGACGACGGGGGGCAGAGCTACGTCGTTTCAGGGAAGCACGGGGCCCCGGAGCCTTATCGTCTCCCAAATGAGGCTTCAATACTCAAGGCTGCTGCCGGTTGGGCCCATTGCGTTTCTGTTACGGAGAATGGTGAATTATACACTTGGGGTTGGAAAGAGTGTGTCCCCTCTGGGAATCTTATCGGTGATCTGGGTATGGTGGGACGCCTTCAAAGGGATACTACCGGTAATCAAAGTTCATTACTAGTTGAACAAGTATCTAATCTTGACAGCAAAAGGGCCAGAGAGGACATTGCAAAGCGAAGGAAGATTTCGTCTGCTAAACCTGAATCTGAAAGTTCAACAGGTAGTGAAGAATTCTTCACTCGGTCTCCTTGTCTTGTAACTCTGGGTCCTGGAGTGAGAATCACTACTGTTGCAGCTGGCGGACGCCACACTTTAGCATTAACAGGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1 RCC1_2

Protein Analysis

235

Amino Acids

24.83

Weight (kDa)

5.71

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 15 - 232 3.3e-14 RCC1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 238
AatII GACGTC 1 cut(s) 177
AccBSI CCGCTC 1 cut(s) 49
AccI GTMKAC 1 cut(s) 242
AciI CCGC 5 cut(s) 49, 180, 183, 186, 672
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 602
AcyI GRCGYC 4 cut(s) 125, 174, 451, 676
AfiI CCNNNNNNNGG 3 cut(s) 252, 316, 460
AgeI ACCGGT 1 cut(s) 470
AgsI TTSAA 4 cut(s) 324, 458, 497, 588
AhlI ACTAGT 1 cut(s) 490
AjnI CCWGG 1 cut(s) 640
AjuI GAANNNNNNNTTGG 2 cut(s) 304, 336
AluBI AGCT 3 cut(s) 59, 264, 668
AluI AGCT 3 cut(s) 59, 264, 668
Alw26I GTCTC 4 cut(s) 111, 123, 311, 621
AoxI GGCC 4 cut(s) 204, 288, 348, 525
ApaI GGGCCC 2 cut(s) 292, 352
ApeKI GCWGC 3 cut(s) 335, 338, 665
ApoI RAATTY 1 cut(s) 602
AsiGI ACCGGT 1 cut(s) 470
AspS9I GGNCC 6 cut(s) 288, 289, 348, 349, 525, 638
AsuC2I CCSGG 2 cut(s) 87, 293
AsuHPI GGTGA 2 cut(s) 389, 443
AvaII GGWCC 1 cut(s) 638
BaeGI GKGCMC 2 cut(s) 292, 352
BalI TGGCCA 1 cut(s) 206
BanII GRGCYC 2 cut(s) 292, 352
BbvI GCAGC 3 cut(s) 322, 325, 677
BccI CCATC 2 cut(s) 142, 215
BciT130I CCWGG 1 cut(s) 642
BciVI GTATCC 1 cut(s) 457
BcnI CCSGG 2 cut(s) 87, 293
BcoDI GTCTC 4 cut(s) 111, 123, 311, 621
BcuI ACTAGT 1 cut(s) 490
BfaI CTAG 1 cut(s) 491
BfuI GTATCC 1 cut(s) 457
BglI GCCNNNNNGGC 1 cut(s) 347
BisI GCNGC 5 cut(s) 181, 184, 336, 339, 666
BlsI GCNGC 5 cut(s) 182, 185, 337, 340, 667
Bme1390I CCNGG 3 cut(s) 87, 293, 642
Bme18I GGWCC 1 cut(s) 638
BmgT120I GGNCC 6 cut(s) 288, 289, 348, 349, 525, 638
BmiI GGNNCC 6 cut(s) 289, 290, 291, 297, 350, 639
BmrFI CCNGG 3 cut(s) 87, 293, 642
BpmI CTGGAG 1 cut(s) 663
BpuEI CTTGAG 1 cut(s) 314
BpuMI CCSGG 2 cut(s) 87, 293
BsaHI GRCGYC 4 cut(s) 125, 174, 451, 676
BsaI GGTCTC 1 cut(s) 621
BsaJI CCNNGG 2 cut(s) 201, 291
BsaWI WCCGGW 1 cut(s) 470
BsaXI ACNNNNNCTCC 2 cut(s) 601, 631
Bsc4I CCNNNNNNNGG 3 cut(s) 252, 316, 460
Bse118I RCCGGY 3 cut(s) 127, 341, 470
Bse3DI GCAATG 3 cut(s) 16, 352, 537
BseBI CCWGG 1 cut(s) 642
BseDI CCNNGG 2 cut(s) 201, 291
BseGI GGATG 1 cut(s) 153
BseLI CCNNNNNNNGG 3 cut(s) 252, 316, 460
BseMI GCAATG 3 cut(s) 16, 352, 537
BseMII CTCAG 1 cut(s) 225
BseRI GAGGAG 2 cut(s) 39, 59
BseSI GKGCMC 2 cut(s) 292, 352
BseXI GCAGC 3 cut(s) 322, 325, 677
BsgI GTGCAG 1 cut(s) 152
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 4 cut(s) 206, 290, 350, 527
BshTI ACCGGT 1 cut(s) 470
BsiEI CGRYCG 1 cut(s) 246
BsiSI CCGG 5 cut(s) 87, 128, 293, 342, 471
BslFI GGGAC 2 cut(s) 395, 462
BslI CCNNNNNNNGG 3 cut(s) 252, 316, 460
BsmAI GTCTC 4 cut(s) 111, 123, 311, 621
BsmBI CGTCTC 1 cut(s) 311
BsmFI GGGAC 2 cut(s) 395, 462
BsnI GGCC 4 cut(s) 206, 290, 350, 527
Bso31I GGTCTC 1 cut(s) 621
Bsp120I GGGCCC 2 cut(s) 288, 348
Bsp1286I GDGCHC 2 cut(s) 292, 352
Bsp143I GATC 1 cut(s) 433
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 5 cut(s) 49, 180, 183, 186, 672
BspANI GGCC 4 cut(s) 206, 290, 350, 527
BspCNI CTCAG 1 cut(s) 224
BspLI GGNNCC 6 cut(s) 289, 290, 291, 297, 350, 639
BspTNI GGTCTC 1 cut(s) 621
BsrBI CCGCTC 1 cut(s) 49
BsrDI GCAATG 3 cut(s) 16, 352, 537
BsrFI RCCGGY 3 cut(s) 127, 341, 470
BssAI RCCGGY 3 cut(s) 127, 341, 470
BssECI CCNNGG 2 cut(s) 201, 291
BssMI GATC 1 cut(s) 433
BssNI GRCGYC 4 cut(s) 125, 174, 451, 676
BssT1I CCWWGG 1 cut(s) 201
Bst2UI CCWGG 1 cut(s) 642
Bst4CI ACNGT 3 cut(s) 117, 155, 661
Bst6I CTCTTC 1 cut(s) 16
BstACI GRCGYC 4 cut(s) 125, 174, 451, 676
BstC8I GCNNGC 1 cut(s) 670
BstDEI CTNAG 1 cut(s) 211
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 153
BstKTI GATC 1 cut(s) 436
BstMAI GTCTC 4 cut(s) 111, 123, 311, 621
BstMBI GATC 1 cut(s) 433
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 1 cut(s) 347
BstNI CCWGG 1 cut(s) 642
BstSCI CCNGG 3 cut(s) 85, 291, 640
BstSLI GKGCMC 2 cut(s) 292, 352
BstV1I GCAGC 3 cut(s) 322, 325, 677
BsuI GTATCC 1 cut(s) 457
BsuRI GGCC 4 cut(s) 206, 290, 350, 527
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 1 cut(s) 153
BtsIMutI CAGTG 1 cut(s) 113
Cac8I GCNNGC 1 cut(s) 670
Cfr10I RCCGGY 3 cut(s) 127, 341, 470
Cfr13I GGNCC 6 cut(s) 288, 289, 348, 349, 525, 638
CseI GACGC 3 cut(s) 133, 459, 684
CspAI ACCGGT 1 cut(s) 470
CviAII CATG 2 cut(s) 202, 234
DdeI CTNAG 1 cut(s) 211
DpnI GATC 1 cut(s) 435
DpnII GATC 1 cut(s) 433
DrdI GACNNNNNNGTC 1 cut(s) 238
DseDI GACNNNNNNGTC 1 cut(s) 238
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
EciI GGCGGA 1 cut(s) 687
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 2 cut(s) 292, 352
Eco31I GGTCTC 1 cut(s) 621
Eco32I GATATC 1 cut(s) 80
Eco47I GGWCC 1 cut(s) 638
EcoO109I RGGNCCY 3 cut(s) 288, 289, 638
EcoRI GAATTC 1 cut(s) 602
EcoRII CCWGG 1 cut(s) 640
EcoRV GATATC 1 cut(s) 80
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 2 cut(s) 292, 352
ErhI CCWWGG 1 cut(s) 201
Esp3I CGTCTC 1 cut(s) 311
FaeI CATG 2 cut(s) 205, 237
FaiI YATR 6 cut(s) 69, 71, 203, 235, 385, 443
FaqI GGGAC 2 cut(s) 395, 462
FatI CATG 2 cut(s) 201, 233
FauI CCCGC 1 cut(s) 42
FblI GTMKAC 1 cut(s) 242
Fnu4HI GCNGC 5 cut(s) 181, 184, 336, 339, 666
FokI GGATG 1 cut(s) 160
FriOI GRGCYC 2 cut(s) 292, 352
Fsp4HI GCNGC 5 cut(s) 181, 184, 336, 339, 666
FspBI CTAG 1 cut(s) 491
GluI GCNGC 5 cut(s) 181, 184, 336, 339, 666
GsuI CTGGAG 1 cut(s) 663
HaeIII GGCC 4 cut(s) 206, 290, 350, 527
HapII CCGG 5 cut(s) 87, 128, 293, 342, 471
HgaI GACGC 3 cut(s) 133, 459, 684
Hin1I GRCGYC 4 cut(s) 125, 174, 451, 676
Hin1II CATG 2 cut(s) 205, 237
HincII GTYRAC 1 cut(s) 243
HindII GTYRAC 1 cut(s) 243
HinfI GANTC 6 cut(s) 142, 161, 195, 421, 575, 651
HpaII CCGG 5 cut(s) 87, 128, 293, 342, 471
HphI GGTGA 2 cut(s) 389, 443
Hpy166II GTNNAC 1 cut(s) 243
Hpy188I TCNGA 6 cut(s) 100, 111, 123, 147, 214, 580
Hpy188III TCNNGA 2 cut(s) 139, 512
Hpy8I GTNNAC 1 cut(s) 243
Hpy99I CGWCG 5 cut(s) 37, 100, 251, 254, 272
HpyAV CCTTC 4 cut(s) 8, 163, 464, 543
HpyCH4III ACNGT 3 cut(s) 117, 155, 661
HpyCH4IV ACGT 2 cut(s) 174, 267
HpyCH4V TGCA 4 cut(s) 9, 133, 542, 665
HpyF10VI GCNNNNNNNGC 1 cut(s) 347
HpyF3I CTNAG 1 cut(s) 211
HpySE526I ACGT 2 cut(s) 174, 267
Hsp92I GRCGYC 4 cut(s) 125, 174, 451, 676
Hsp92II CATG 2 cut(s) 205, 237
Kzo9I GATC 1 cut(s) 433
LmnI GCTCC 2 cut(s) 46, 295
Lsp1109I GCAGC 3 cut(s) 322, 325, 677
MaeI CTAG 1 cut(s) 491
MaeII ACGT 2 cut(s) 174, 267
MaeIII GTNAC 2 cut(s) 364, 628
MalI GATC 1 cut(s) 435
MbiI CCGCTC 1 cut(s) 49
MboI GATC 1 cut(s) 433
MboII GAAGA 5 cut(s) 33, 74, 565, 598, 611
MhlI GDGCHC 2 cut(s) 292, 352
MlsI TGGCCA 1 cut(s) 206
MluCI AATT 2 cut(s) 380, 602
MluNI TGGCCA 1 cut(s) 206
MmeI TCCRAC 3 cut(s) 78, 134, 377
MnlI CCTC 7 cut(s) 11, 17, 20, 37, 310, 424, 526
Mox20I TGGCCA 1 cut(s) 206
MscI TGGCCA 1 cut(s) 206
MseI TTAA 2 cut(s) 227, 692
Msp20I TGGCCA 1 cut(s) 206
MspA1I CMGCKG 1 cut(s) 668
MspI CCGG 5 cut(s) 87, 128, 293, 342, 471
MspR9I CCNGG 3 cut(s) 87, 293, 642
MvaI CCWGG 1 cut(s) 642
MwoI GCNNNNNNNGC 1 cut(s) 347
NciI CCSGG 2 cut(s) 87, 293
NcoI CCATGG 1 cut(s) 201
NdeII GATC 1 cut(s) 433
NlaIII CATG 2 cut(s) 205, 237
NlaIV GGNNCC 6 cut(s) 289, 290, 291, 297, 350, 639
PfeI GAWTC 6 cut(s) 142, 161, 195, 421, 575, 651
PflFI GACNNNGTC 1 cut(s) 115
PfoI TCCNGGA 2 cut(s) 85, 640
PinAI ACCGGT 1 cut(s) 470
PkrI GCNGC 5 cut(s) 182, 185, 337, 340, 667
PpuMI RGGWCCY 1 cut(s) 638
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 1 cut(s) 640
PspGI CCWGG 1 cut(s) 640
PspN4I GGNNCC 6 cut(s) 289, 290, 291, 297, 350, 639
PspOMI GGGCCC 2 cut(s) 288, 348
PspPI GGNCC 6 cut(s) 288, 289, 348, 349, 525, 638
PspPPI RGGWCCY 1 cut(s) 638
PsrI GAACNNNNNNTAC 2 cut(s) 466, 498
PsyI GACNNNGTC 1 cut(s) 115
PvuII CAGCTG 1 cut(s) 668
SalI GTCGAC 1 cut(s) 241
SaqAI TTAA 2 cut(s) 227, 692
SatI GCNGC 5 cut(s) 181, 184, 336, 339, 666
Sau3AI GATC 1 cut(s) 433
Sau96I GGNCC 6 cut(s) 288, 289, 348, 349, 525, 638
ScrFI CCNGG 3 cut(s) 87, 293, 642
SduI GDGCHC 2 cut(s) 292, 352
SetI ASST 8 cut(s) 61, 177, 266, 270, 574, 595, 670, 700
SgrAI CRCCGGYG 1 cut(s) 127
SinI GGWCC 1 cut(s) 638
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
SpeI ACTAGT 1 cut(s) 490
Sse9I AATT 2 cut(s) 380, 602
SsiI CCGC 5 cut(s) 49, 180, 183, 186, 672
SspMI CTAG 1 cut(s) 491
StyD4I CCNGG 3 cut(s) 85, 291, 640
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 3 cut(s) 117, 155, 661
TaiI ACGT 2 cut(s) 177, 270
TaqI TCGA 2 cut(s) 32, 242
TaqII GACCGA 2 cut(s) 260, 603
TasI AATT 2 cut(s) 380, 602
TauI GCSGC 2 cut(s) 183, 186
TfiI GAWTC 6 cut(s) 142, 161, 195, 421, 575, 651
Tru1I TTAA 2 cut(s) 227, 692
Tru9I TTAA 2 cut(s) 227, 692
TscAI CASTG 1 cut(s) 120
TseI GCWGC 3 cut(s) 335, 338, 665
TspDTI ATGAA 2 cut(s) 27, 474
TspGWI ACGGA 2 cut(s) 56, 383
TspRI CASTG 1 cut(s) 120
Tth111I GACNNNGTC 1 cut(s) 115
VpaK11BI GGWCC 1 cut(s) 638
XapI RAATTY 1 cut(s) 602
XmiI GTMKAC 1 cut(s) 242
XspI CTAG 1 cut(s) 491
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.