MD12G1149100.v1.1

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007631
Physical Location & Seq
Forward (+)
22906947 .. 22908812
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1149100.v1.1.491

Sequence Viewer

Length: 774 bp
ATGGACTGCAATGAAGGAGGAGGAGGAGGGTCGACGAAAACGGAGGAGCGGAGAGAAGCTCTGGTGTATATGTGGGGATATCTTCCGGGAGTATCGCCGGAGAAGTCTCCGATACTGTCTCTGTCGCCGGTGCGGTTTCCTAATGGGATGGAGGGTGGATATTCGTGGAAGGACGTCTGCGGCGGCGGTTGCGGATTCGCCATGGCCATCTCAGAGTGTGGGAAGCTGATGACGTGGGGGTCGACGGACGAGGAGGGGCAGAGCTACGTCGTTTCTGGGAAGCACGGGACTCCGGAGGCATATGCTCTCCCAACTGAGGCTTCAATACTCAAGGCTGCTGCTGGTTGGGCTCATTGCGTTGCTGTTACGGAGACTGGTGAAGTATACACTTGGGGTTGGAAAGAGTGTGTTCCCTCTGGGAATCTTATTGGTGATCTGGGCATGGTGGAACGTCTCCAAAAGGATACTACAGGGAATCAAAGTTCATTACCAGCTGAACAAGTAAACTCAATGTCTCAAGGCTTCAATTTGACTAGTGGGACAGTATCTCATCTTGAAAGCAAAAGGGCCAGAGAGGAAATTGCAAAGCGAAGGAAAATTTCGTCAGCTAAAGTCGAATCTGAAAGCTCAACAGGTGGTGAAGAATTCTTCAATCCATCCCCTTGTCTTGTAACTCTGGGTCCTGGAGTGAGAATCACCACTGTCGCAGCTGGTGGGCGCCATACTTTAGCATTATCAGGTTGTAGGATGTACGAAGCTATGGAGGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1 RCC1_2

Protein Analysis

258

Amino Acids

27.33

Weight (kDa)

5.16

Isoelectric Point (pI)

55.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 42 - 140 2.2e-13 RCC1-like domain
RCC1_2 PF13540 111 - 133 1.5e-06 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 238
AatII GACGTC 1 cut(s) 177
AccB1I GGYRCC 1 cut(s) 717
AccBSI CCGCTC 1 cut(s) 49
AccI GTMKAC 3 cut(s) 32, 242, 384
AccIII TCCGGA 1 cut(s) 292
AciI CCGC 6 cut(s) 49, 133, 180, 183, 186, 192
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 2 cut(s) 597, 644
AcyI GRCGYC 2 cut(s) 174, 718
AfaI GTAC 1 cut(s) 752
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 4 cut(s) 324, 526, 557, 652
AhlI ACTAGT 1 cut(s) 533
AjiI CACGTC 1 cut(s) 234
AjnI CCWGG 1 cut(s) 682
AjuI GAANNNNNNNTTGG 2 cut(s) 304, 336
AloI GAACNNNNNNTCC 2 cut(s) 466, 498
AluBI AGCT 8 cut(s) 59, 226, 264, 494, 608, 627, 710, 758
AluI AGCT 8 cut(s) 59, 226, 264, 494, 608, 627, 710, 758
Alw26I GTCTC 5 cut(s) 111, 123, 365, 458, 519
Aor13HI TCCGGA 1 cut(s) 292
AoxI GGCC 2 cut(s) 204, 567
ApeKI GCWGC 3 cut(s) 335, 338, 707
ApoI RAATTY 2 cut(s) 597, 644
AspLEI GCGC 1 cut(s) 720
AspS9I GGNCC 2 cut(s) 567, 680
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 4 cut(s) 389, 443, 650, 688
AvaII GGWCC 1 cut(s) 680
BalI TGGCCA 1 cut(s) 206
BanI GGYRCC 1 cut(s) 717
BanII GRGCYC 1 cut(s) 352
BbvI GCAGC 3 cut(s) 322, 325, 719
BccI CCATC 3 cut(s) 142, 215, 664
BciT130I CCWGG 1 cut(s) 684
BciVI GTATCC 1 cut(s) 457
BcnI CCSGG 1 cut(s) 87
BcoDI GTCTC 5 cut(s) 111, 123, 365, 458, 519
BcuI ACTAGT 1 cut(s) 533
BfaI CTAG 1 cut(s) 534
BfmI CTRYAG 1 cut(s) 468
BfoI RGCGCY 1 cut(s) 721
BfuI GTATCC 1 cut(s) 457
BisI GCNGC 5 cut(s) 181, 184, 336, 339, 708
BlsI GCNGC 5 cut(s) 182, 185, 337, 340, 709
Bme1390I CCNGG 2 cut(s) 87, 684
Bme18I GGWCC 1 cut(s) 680
BmgBI CACGTC 1 cut(s) 234
BmgT120I GGNCC 2 cut(s) 567, 680
BmiI GGNNCC 2 cut(s) 681, 719
BmrFI CCNGG 2 cut(s) 87, 684
BplI GAGNNNNNCTC 2 cut(s) 43, 75
BpmI CTGGAG 1 cut(s) 705
BpuEI CTTGAG 2 cut(s) 314, 501
BpuMI CCSGG 1 cut(s) 87
BsaHI GRCGYC 2 cut(s) 174, 718
BsaJI CCNNGG 1 cut(s) 201
BsaWI WCCGGW 1 cut(s) 292
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse118I RCCGGY 1 cut(s) 127
Bse1I ACTGG 1 cut(s) 379
Bse3DI GCAATG 2 cut(s) 16, 352
BseAI TCCGGA 1 cut(s) 292
BseBI CCWGG 1 cut(s) 684
BseDI CCNNGG 1 cut(s) 201
BseGI GGATG 3 cut(s) 153, 656, 753
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMI GCAATG 2 cut(s) 16, 352
BseMII CTCAG 2 cut(s) 225, 306
BseNI ACTGG 1 cut(s) 379
BseRI GAGGAG 5 cut(s) 33, 36, 39, 59, 266
BseXI GCAGC 3 cut(s) 322, 325, 719
BshFI GGCC 2 cut(s) 206, 569
BshNI GGYRCC 1 cut(s) 717
BsiSI CCGG 4 cut(s) 86, 98, 128, 293
BslFI GGGAC 2 cut(s) 301, 553
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 5 cut(s) 111, 123, 365, 458, 519
BsmBI CGTCTC 1 cut(s) 458
BsmFI GGGAC 2 cut(s) 301, 553
BsnI GGCC 2 cut(s) 206, 569
Bsp1286I GDGCHC 1 cut(s) 352
Bsp13I TCCGGA 1 cut(s) 292
Bsp143I GATC 1 cut(s) 433
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 6 cut(s) 49, 133, 180, 183, 186, 192
BspANI GGCC 2 cut(s) 206, 569
BspCNI CTCAG 2 cut(s) 224, 307
BspEI TCCGGA 1 cut(s) 292
BspLI GGNNCC 2 cut(s) 681, 719
BspT107I GGYRCC 1 cut(s) 717
BsrBI CCGCTC 1 cut(s) 49
BsrDI GCAATG 2 cut(s) 16, 352
BsrFI RCCGGY 1 cut(s) 127
BsrI ACTGG 1 cut(s) 379
BssAI RCCGGY 1 cut(s) 127
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 1 cut(s) 433
BssNAI GTATAC 1 cut(s) 385
BssNI GRCGYC 2 cut(s) 174, 718
BssT1I CCWWGG 1 cut(s) 201
Bst1107I GTATAC 1 cut(s) 385
Bst2UI CCWGG 1 cut(s) 684
Bst4CI ACNGT 3 cut(s) 117, 544, 703
BstACI GRCGYC 2 cut(s) 174, 718
BstDEI CTNAG 2 cut(s) 211, 315
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 3 cut(s) 153, 656, 753
BstH2I RGCGCY 1 cut(s) 721
BstHHI GCGC 1 cut(s) 720
BstKTI GATC 1 cut(s) 436
BstMAI GTCTC 5 cut(s) 111, 123, 365, 458, 519
BstMBI GATC 1 cut(s) 433
BstMWI GCNNNNNNNGC 2 cut(s) 189, 347
BstNI CCWGG 1 cut(s) 684
BstSCI CCNGG 2 cut(s) 85, 682
BstSFI CTRYAG 1 cut(s) 468
BstV1I GCAGC 3 cut(s) 322, 325, 719
BstZ17I GTATAC 1 cut(s) 385
BsuI GTATCC 1 cut(s) 457
BsuRI GGCC 2 cut(s) 206, 569
BtgI CCRYGG 1 cut(s) 201
BtrI CACGTC 1 cut(s) 234
BtsCI GGATG 3 cut(s) 153, 656, 753
BtsIMutI CAGTG 1 cut(s) 699
CfoI GCGC 1 cut(s) 720
Cfr10I RCCGGY 1 cut(s) 127
Cfr13I GGNCC 2 cut(s) 567, 680
Csp6I GTAC 1 cut(s) 751
CviAII CATG 2 cut(s) 202, 442
CviQI GTAC 1 cut(s) 751
DdeI CTNAG 2 cut(s) 211, 315
DinI GGCGCC 1 cut(s) 719
DpnI GATC 1 cut(s) 435
DpnII GATC 1 cut(s) 433
DrdI GACNNNNNNGTC 1 cut(s) 238
DseDI GACNNNNNNGTC 1 cut(s) 238
EaeI YGGCCR 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 352
Eco32I GATATC 1 cut(s) 80
Eco47I GGWCC 1 cut(s) 680
EcoO109I RGGNCCY 1 cut(s) 680
EcoRI GAATTC 1 cut(s) 644
EcoRII CCWGG 1 cut(s) 682
EcoRV GATATC 1 cut(s) 80
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 352
EgeI GGCGCC 1 cut(s) 719
EheI GGCGCC 1 cut(s) 719
ErhI CCWWGG 1 cut(s) 201
Esp3I CGTCTC 1 cut(s) 458
FaeI CATG 2 cut(s) 205, 445
FaiI YATR 9 cut(s) 69, 71, 203, 301, 303, 385, 443, 723, 761
FaqI GGGAC 2 cut(s) 301, 553
FatI CATG 2 cut(s) 201, 441
FauNDI CATATG 1 cut(s) 301
FblI GTMKAC 3 cut(s) 32, 242, 384
Fnu4HI GCNGC 5 cut(s) 181, 184, 336, 339, 708
FokI GGATG 3 cut(s) 160, 643, 760
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 5 cut(s) 181, 184, 336, 339, 708
FspBI CTAG 1 cut(s) 534
GlaI GCGC 1 cut(s) 719
GluI GCNGC 5 cut(s) 181, 184, 336, 339, 708
GsuI CTGGAG 1 cut(s) 705
HaeII RGCGCY 1 cut(s) 721
HaeIII GGCC 2 cut(s) 206, 569
HapII CCGG 4 cut(s) 86, 98, 128, 293
HhaI GCGC 1 cut(s) 720
Hin1I GRCGYC 2 cut(s) 174, 718
Hin1II CATG 2 cut(s) 205, 445
Hin6I GCGC 1 cut(s) 718
HinP1I GCGC 1 cut(s) 718
HincII GTYRAC 2 cut(s) 33, 243
HindII GTYRAC 2 cut(s) 33, 243
HinfI GANTC 6 cut(s) 195, 289, 421, 475, 617, 693
HpaII CCGG 4 cut(s) 86, 98, 128, 293
HphI GGTGA 4 cut(s) 389, 443, 650, 688
Hpy166II GTNNAC 4 cut(s) 33, 243, 385, 505
Hpy188I TCNGA 3 cut(s) 111, 214, 622
Hpy188III TCNNGA 2 cut(s) 293, 554
Hpy8I GTNNAC 4 cut(s) 33, 243, 385, 505
Hpy99I CGWCG 3 cut(s) 37, 247, 272
HpyAV CCTTC 3 cut(s) 8, 163, 585
HpyCH4III ACNGT 3 cut(s) 117, 544, 703
HpyCH4IV ACGT 4 cut(s) 174, 233, 267, 451
HpyCH4V TGCA 2 cut(s) 9, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 189, 347
HpyF3I CTNAG 2 cut(s) 211, 315
HpySE526I ACGT 4 cut(s) 174, 233, 267, 451
Hsp92I GRCGYC 2 cut(s) 174, 718
Hsp92II CATG 2 cut(s) 205, 445
HspAI GCGC 1 cut(s) 718
KasI GGCGCC 1 cut(s) 717
Kpn2I TCCGGA 1 cut(s) 292
Kzo9I GATC 1 cut(s) 433
LmnI GCTCC 1 cut(s) 46
Lsp1109I GCAGC 3 cut(s) 322, 325, 719
MaeI CTAG 1 cut(s) 534
MaeII ACGT 4 cut(s) 174, 233, 267, 451
MaeIII GTNAC 2 cut(s) 364, 670
MalI GATC 1 cut(s) 435
MbiI CCGCTC 1 cut(s) 49
MboI GATC 1 cut(s) 433
MboII GAAGA 3 cut(s) 74, 640, 653
MhlI GDGCHC 1 cut(s) 352
MlsI TGGCCA 1 cut(s) 206
MluCI AATT 4 cut(s) 526, 579, 597, 644
MluNI TGGCCA 1 cut(s) 206
Mly113I GGCGCC 1 cut(s) 718
MlyI GAGTC 1 cut(s) 283
MmeI TCCRAC 1 cut(s) 377
Mox20I TGGCCA 1 cut(s) 206
MroI TCCGGA 1 cut(s) 292
MscI TGGCCA 1 cut(s) 206
Msp20I TGGCCA 1 cut(s) 206
MspA1I CMGCKG 2 cut(s) 494, 710
MspI CCGG 4 cut(s) 86, 98, 128, 293
MspR9I CCNGG 2 cut(s) 87, 684
MvaI CCWGG 1 cut(s) 684
MwoI GCNNNNNNNGC 2 cut(s) 189, 347
NarI GGCGCC 1 cut(s) 718
NciI CCSGG 1 cut(s) 87
NcoI CCATGG 1 cut(s) 201
NdeI CATATG 1 cut(s) 301
NdeII GATC 1 cut(s) 433
NlaIII CATG 2 cut(s) 205, 445
NlaIV GGNNCC 2 cut(s) 681, 719
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PfeI GAWTC 5 cut(s) 195, 421, 475, 617, 693
PfoI TCCNGGA 2 cut(s) 85, 682
PkrI GCNGC 5 cut(s) 182, 185, 337, 340, 709
PleI GAGTC 1 cut(s) 283
PluTI GGCGCC 1 cut(s) 721
PpsI GAGTC 1 cut(s) 283
PpuMI RGGWCCY 1 cut(s) 680
Psp5II RGGWCCY 1 cut(s) 680
Psp6I CCWGG 1 cut(s) 682
PspGI CCWGG 1 cut(s) 682
PspN4I GGNNCC 2 cut(s) 681, 719
PspPI GGNCC 2 cut(s) 567, 680
PspPPI RGGWCCY 1 cut(s) 680
PvuII CAGCTG 2 cut(s) 494, 710
RsaI GTAC 1 cut(s) 752
RsaNI GTAC 1 cut(s) 751
SalI GTCGAC 2 cut(s) 31, 241
SatI GCNGC 5 cut(s) 181, 184, 336, 339, 708
Sau3AI GATC 1 cut(s) 433
Sau96I GGNCC 2 cut(s) 567, 680
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 2 cut(s) 87, 684
SduI GDGCHC 1 cut(s) 352
SfcI CTRYAG 1 cut(s) 468
SfoI GGCGCC 1 cut(s) 719
SgrAI CRCCGGYG 1 cut(s) 127
SinI GGWCC 1 cut(s) 680
SmlI CTYRAG 2 cut(s) 329, 516
SmoI CTYRAG 2 cut(s) 329, 516
SpeI ACTAGT 1 cut(s) 533
Sse9I AATT 4 cut(s) 526, 579, 597, 644
SsiI CCGC 6 cut(s) 49, 133, 180, 183, 186, 192
SspDI GGCGCC 1 cut(s) 717
SspMI CTAG 1 cut(s) 534
StyD4I CCNGG 2 cut(s) 85, 682
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 3 cut(s) 117, 544, 703
TaiI ACGT 4 cut(s) 177, 236, 270, 454
TaqI TCGA 3 cut(s) 32, 242, 615
TasI AATT 4 cut(s) 526, 579, 597, 644
TauI GCSGC 2 cut(s) 183, 186
TfiI GAWTC 5 cut(s) 195, 421, 475, 617, 693
TscAI CASTG 1 cut(s) 706
TseI GCWGC 3 cut(s) 335, 338, 707
TspDTI ATGAA 2 cut(s) 27, 474
TspGWI ACGGA 3 cut(s) 56, 260, 383
TspRI CASTG 1 cut(s) 706
VpaK11BI GGWCC 1 cut(s) 680
XapI RAATTY 2 cut(s) 597, 644
XmiI GTMKAC 3 cut(s) 32, 242, 384
XspI CTAG 1 cut(s) 534
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.