MD04G1135500.v1.1

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007631
Physical Location & Seq
Forward (+)
22244004 .. 22245199
1196 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1135500.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGGACAGGTGTGGGGTTGGGGCTATGGAGGCGAAGGACAGCTAGGTTTGGGTACCAGGGTAAAGATGGTTTCTTCTCCTCATGTCATACCCTGTATTGAGCCATCTACTTCTGGGAAGGATAGGTCTTCAGTGGTATCTCAAGGTTCAAAGGTTCCTGGAAGTTGTGTGAAGGAGATTGCTTGTGGAGGCCGGCACAGTGCAGTAATAACAGATGCTGGAGCACTGCTTACGTTTGGCTGGGGGCTCTATGGACAGTGTGGGCAAGGGAATACAAATGATCAGCTAAGACCAAGTTATCTGAATTCATTATCCGGTACTAAAGTGAAAAACATCGCTGCCGGACTATGGCATACTCTATGTATATCTGATGATGGTCGTGTACATGCTTTTGGTGGGAATCAGTTTGGACAGTTGGGAACAGGTGGCGACGAGGCTGAGACTCTACCTAAGATATTGGATTTTCCAGGTTTGGGAAGCAAGCATGCAAAAGTAGCCTCCTGCGGCGCCCGACATAGTGTCATTCTAACAGGTAAGCAAATGAAGCTCAAAAGCGTTGATACTTGCACTAATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1 RCC1_2

Protein Analysis

193

Amino Acids

19.93

Weight (kDa)

8.81

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 2 - 179 1.5e-32 RCC1-like domain
RCC1 PF00415 2 - 70 1e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 57 - 86 2e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 74 - 122 1.3e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 109 - 138 2e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 125 - 176 1e-10 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 53
AccB1I GGYRCC 2 cut(s) 53, 506
AciI CCGC 1 cut(s) 504
AcsI RAATTY 1 cut(s) 304
AcuI CTGAAG 1 cut(s) 114
AcyI GRCGYC 1 cut(s) 507
AfaI GTAC 3 cut(s) 55, 319, 384
AfiI CCNNNNNNNGG 2 cut(s) 348, 473
AgsI TTSAA 1 cut(s) 150
AhdI GACNNNNNGTC 1 cut(s) 518
AjnI CCWGG 3 cut(s) 56, 157, 466
AluBI AGCT 3 cut(s) 43, 286, 547
AluI AGCT 3 cut(s) 43, 286, 547
Alw21I GWGCWC 1 cut(s) 226
Alw26I GTCTC 1 cut(s) 434
AlwNI CAGNNNCTG 1 cut(s) 218
AoxI GGCC 1 cut(s) 190
ApeKI GCWGC 1 cut(s) 338
ApoI RAATTY 1 cut(s) 304
Asp718I GGTACC 1 cut(s) 53
AspLEI GCGC 1 cut(s) 509
BaeI ACNNNNGTAYC 4 cut(s) 45, 78, 309, 342
BanI GGYRCC 2 cut(s) 53, 506
BanII GRGCYC 1 cut(s) 249
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 226
BbvI GCAGC 1 cut(s) 325
BccI CCATC 3 cut(s) 61, 112, 368
BciT130I CCWGG 3 cut(s) 58, 159, 468
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 434
BfaI CTAG 1 cut(s) 44
BfoI RGCGCY 1 cut(s) 510
BisI GCNGC 2 cut(s) 339, 505
BlsI GCNGC 2 cut(s) 340, 506
Bme1390I CCNGG 3 cut(s) 58, 159, 468
BmeRI GACNNNNNGTC 1 cut(s) 518
BmiI GGNNCC 3 cut(s) 55, 156, 508
BmrFI CCNGG 3 cut(s) 58, 159, 468
BmsI GCATC 1 cut(s) 205
BpiI GAAGAC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 126
BsaHI GRCGYC 1 cut(s) 507
BsaJI CCNNGG 1 cut(s) 57
BsaWI WCCGGW 1 cut(s) 314
Bsc4I CCNNNNNNNGG 2 cut(s) 348, 473
Bse118I RCCGGY 1 cut(s) 192
BseBI CCWGG 3 cut(s) 58, 159, 468
BseDI CCNNGG 1 cut(s) 57
BseLI CCNNNNNNNGG 2 cut(s) 348, 473
BseMII CTCAG 1 cut(s) 429
BseRI GAGGAG 1 cut(s) 69
BseXI GCAGC 1 cut(s) 325
BseYI CCCAGC 1 cut(s) 240
BsgI GTGCAG 1 cut(s) 222
BshFI GGCC 1 cut(s) 192
BshNI GGYRCC 2 cut(s) 53, 506
BsiHKAI GWGCWC 1 cut(s) 226
BsiSI CCGG 3 cut(s) 193, 315, 342
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 2 cut(s) 348, 473
BsmAI GTCTC 1 cut(s) 434
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 1 cut(s) 192
Bsp1286I GDGCHC 2 cut(s) 226, 249
Bsp1407I TGTACA 1 cut(s) 382
Bsp143I GATC 1 cut(s) 280
BspACI CCGC 1 cut(s) 504
BspANI GGCC 1 cut(s) 192
BspCNI CTCAG 1 cut(s) 430
BspLI GGNNCC 3 cut(s) 55, 156, 508
BspT107I GGYRCC 2 cut(s) 53, 506
BsrFI RCCGGY 1 cut(s) 192
BsrGI TGTACA 1 cut(s) 382
BssAI RCCGGY 1 cut(s) 192
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 1 cut(s) 280
BssNI GRCGYC 1 cut(s) 507
Bst2UI CCWGG 3 cut(s) 58, 159, 468
Bst4CI ACNGT 3 cut(s) 200, 258, 414
BstACI GRCGYC 1 cut(s) 507
BstAPI GCANNNNNTGC 1 cut(s) 573
BstAUI TGTACA 1 cut(s) 382
BstC8I GCNNGC 3 cut(s) 194, 482, 486
BstDEI CTNAG 3 cut(s) 287, 438, 450
BstH2I RGCGCY 1 cut(s) 510
BstHHI GCGC 1 cut(s) 509
BstKTI GATC 1 cut(s) 283
BstMAI GTCTC 1 cut(s) 434
BstMBI GATC 1 cut(s) 280
BstMWI GCNNNNNNNGC 4 cut(s) 30, 494, 544, 573
BstNI CCWGG 3 cut(s) 58, 159, 468
BstNSI RCATGY 2 cut(s) 389, 488
BstSCI CCNGG 3 cut(s) 56, 157, 466
BstV1I GCAGC 1 cut(s) 325
BstV2I GAAGAC 1 cut(s) 120
BsuRI GGCC 1 cut(s) 192
BtgZI GCGATG 1 cut(s) 319
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 4 cut(s) 138, 205, 224, 263
Cac8I GCNNGC 3 cut(s) 194, 482, 486
CaiI CAGNNNCTG 1 cut(s) 218
CfoI GCGC 1 cut(s) 509
Cfr10I RCCGGY 1 cut(s) 192
Csp6I GTAC 3 cut(s) 54, 318, 383
CviAII CATG 3 cut(s) 83, 386, 485
CviQI GTAC 3 cut(s) 54, 318, 383
DdeI CTNAG 3 cut(s) 287, 438, 450
DinI GGCGCC 1 cut(s) 508
DpnI GATC 1 cut(s) 282
DpnII GATC 1 cut(s) 280
DriI GACNNNNNGTC 1 cut(s) 518
Eam1105I GACNNNNNGTC 1 cut(s) 518
Eco24I GRGCYC 1 cut(s) 249
Eco57I CTGAAG 1 cut(s) 114
EcoRI GAATTC 1 cut(s) 304
EcoRII CCWGG 3 cut(s) 56, 157, 466
EcoT38I GRGCYC 1 cut(s) 249
EgeI GGCGCC 1 cut(s) 508
EheI GGCGCC 1 cut(s) 508
FaeI CATG 3 cut(s) 86, 389, 488
FaqI GGGAC 1 cut(s) 18
FatI CATG 3 cut(s) 82, 385, 484
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 2 cut(s) 339, 505
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 2 cut(s) 339, 505
FspBI CTAG 1 cut(s) 44
GlaI GCGC 1 cut(s) 508
GluI GCNGC 2 cut(s) 339, 505
GsaI CCCAGC 1 cut(s) 244
GsuI CTGGAG 1 cut(s) 240
HaeII RGCGCY 1 cut(s) 510
HaeIII GGCC 1 cut(s) 192
HapII CCGG 3 cut(s) 193, 315, 342
HhaI GCGC 1 cut(s) 509
Hin1I GRCGYC 1 cut(s) 507
Hin1II CATG 3 cut(s) 86, 389, 488
Hin6I GCGC 1 cut(s) 507
HinP1I GCGC 1 cut(s) 507
HinfI GANTC 2 cut(s) 400, 442
HpaII CCGG 3 cut(s) 193, 315, 342
Hpy166II GTNNAC 1 cut(s) 383
Hpy188I TCNGA 2 cut(s) 303, 370
Hpy8I GTNNAC 1 cut(s) 383
Hpy99I CGWCG 1 cut(s) 434
HpyAV CCTTC 3 cut(s) 29, 112, 166
HpyCH4III ACNGT 3 cut(s) 200, 258, 414
HpyCH4IV ACGT 1 cut(s) 233
HpyCH4V TGCA 3 cut(s) 203, 488, 567
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 494, 544, 573
HpyF3I CTNAG 3 cut(s) 287, 438, 450
HpySE526I ACGT 1 cut(s) 233
Hsp92I GRCGYC 1 cut(s) 507
Hsp92II CATG 3 cut(s) 86, 389, 488
HspAI GCGC 1 cut(s) 507
KasI GGCGCC 1 cut(s) 506
KpnI GGTACC 1 cut(s) 57
KroI GCCGGC 1 cut(s) 192
KroNI GCCGGC 1 cut(s) 194
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 1 cut(s) 280
LmnI GCTCC 1 cut(s) 221
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 1 cut(s) 205
MaeI CTAG 1 cut(s) 44
MaeII ACGT 1 cut(s) 233
MalI GATC 1 cut(s) 282
MboI GATC 1 cut(s) 280
MboII GAAGA 2 cut(s) 66, 120
MhlI GDGCHC 2 cut(s) 226, 249
MluCI AATT 2 cut(s) 304, 571
Mly113I GGCGCC 1 cut(s) 507
MlyI GAGTC 1 cut(s) 436
MnlI CCTC 5 cut(s) 23, 90, 182, 427, 508
MroNI GCCGGC 1 cut(s) 192
MspI CCGG 3 cut(s) 193, 315, 342
MspR9I CCNGG 3 cut(s) 58, 159, 468
MvaI CCWGG 3 cut(s) 58, 159, 468
MwoI GCNNNNNNNGC 4 cut(s) 30, 494, 544, 573
NaeI GCCGGC 1 cut(s) 194
NarI GGCGCC 1 cut(s) 507
NdeII GATC 1 cut(s) 280
NgoMIV GCCGGC 1 cut(s) 192
NlaIII CATG 3 cut(s) 86, 389, 488
NlaIV GGNNCC 3 cut(s) 55, 156, 508
NspI RCATGY 2 cut(s) 389, 488
PaeI GCATGC 1 cut(s) 488
PdiI GCCGGC 1 cut(s) 194
PfeI GAWTC 1 cut(s) 400
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 2 cut(s) 340, 506
PleI GAGTC 1 cut(s) 436
PluTI GGCGCC 1 cut(s) 510
PpsI GAGTC 1 cut(s) 436
Psp6I CCWGG 3 cut(s) 56, 157, 466
PspFI CCCAGC 1 cut(s) 240
PspGI CCWGG 3 cut(s) 56, 157, 466
PspN4I GGNNCC 3 cut(s) 55, 156, 508
PstNI CAGNNNCTG 1 cut(s) 218
RsaI GTAC 3 cut(s) 55, 319, 384
RsaNI GTAC 3 cut(s) 54, 318, 383
SatI GCNGC 2 cut(s) 339, 505
Sau3AI GATC 1 cut(s) 280
SchI GAGTC 1 cut(s) 436
ScrFI CCNGG 3 cut(s) 58, 159, 468
SduI GDGCHC 2 cut(s) 226, 249
SfaNI GCATC 1 cut(s) 205
SfoI GGCGCC 1 cut(s) 508
SmlI CTYRAG 1 cut(s) 141
SmoI CTYRAG 1 cut(s) 141
SphI GCATGC 1 cut(s) 488
Sse9I AATT 2 cut(s) 304, 571
SsiI CCGC 1 cut(s) 504
SspDI GGCGCC 1 cut(s) 506
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 3 cut(s) 56, 157, 466
TaaI ACNGT 3 cut(s) 200, 258, 414
TaiI ACGT 1 cut(s) 236
TasI AATT 2 cut(s) 304, 571
TatI WGTACW 1 cut(s) 382
TauI GCSGC 1 cut(s) 507
TfiI GAWTC 1 cut(s) 400
TscAI CASTG 4 cut(s) 138, 205, 231, 263
TseI GCWGC 1 cut(s) 338
TspDTI ATGAA 2 cut(s) 297, 557
TspRI CASTG 4 cut(s) 138, 205, 231, 263
XapI RAATTY 1 cut(s) 304
XceI RCATGY 2 cut(s) 389, 488
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.