MD12G1149200.v1.1

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007631
Physical Location & Seq
Forward (+)
22908814 .. 22910690
1877 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1149200.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGGACAGGTGTGGGGTTGGGGCTATGGAGGCGAAGGACAGCTAGGTTTGGGTACCAGGGTAAAAATGGTTTCTTCTCCTCATGTCATCCCCTGTATTCAGCCATCTGCGTCTGGGAAGGATAGGTCTTCTGTGATATCTCAAGGTTCAAAAATTCCTGGAAGTTATGTGAAGGAGATTGCTTGTGGAGGCCGGCACAGTGCAGTAATAACAGATGCTGGAGCACTGCTTACTTTTGGCTGGGGGCTTTACGGACAGTGTGGGCAAGGGAATACAATAGATCAGCTAAGACCAAGTTATGTGAAATCATTGTCCGAAACAAAAGTGAAAAACATTGCTGCCGGACTATGGCATACACTGTGTGTATCTGTTGACGGGCGTGTATATGCATTTGGAGGGAATCAGTTTGGACAGCTGGGAACAGGTGCTGACGAGGGTGAGCTTCAGACTCTACCTAAGATATTGGATTCTCCGGGTTTGGGAAGCAAGCATGCAAAAGTAGCCTCCTGCGGCGCTCGACATAGTGTCATTCTAACAGAAGACGGCCAGTTATTTAGTTGGGGGTGGAACAAGTATGGCCAACTTGGCCTGGGCGATGCCGTGGATAGGAACATTCCTTCTCAAGTTTCAATAGAAGGCTGCTTACTGAAAAATATTGCATGTGGCTGGTGGCACACGCTACTTCTAGCCGAAAGACCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1 RCC1_2

Protein Analysis

235

Amino Acids

24.75

Weight (kDa)

8.46

Isoelectric Point (pI)

43.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 2 - 230 2.6e-49 RCC1-like domain
RCC1 PF00415 2 - 70 7.3e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 57 - 86 2.7e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 74 - 122 1.2e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 109 - 138 8.4e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 125 - 178 1e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 169 - 194 4.1e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 181 - 229 6.1e-15 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 53
AccB1I GGYRCC 1 cut(s) 53
AciI CCGC 1 cut(s) 510
AcoI YGGCCR 2 cut(s) 544, 577
AcsI RAATTY 1 cut(s) 153
AcuI CTGAAG 1 cut(s) 428
AdeI CACNNNGTG 1 cut(s) 362
AfaI GTAC 1 cut(s) 55
AfiI CCNNNNNNNGG 3 cut(s) 348, 479, 606
AgsI TTSAA 2 cut(s) 150, 630
AhdI GACNNNNNGTC 1 cut(s) 524
AjnI CCWGG 3 cut(s) 56, 157, 588
AluBI AGCT 4 cut(s) 43, 286, 415, 442
AluI AGCT 4 cut(s) 43, 286, 415, 442
Alw21I GWGCWC 1 cut(s) 226
AlwNI CAGNNNCTG 2 cut(s) 218, 428
AoxI GGCC 4 cut(s) 190, 544, 577, 586
ApeKI GCWGC 2 cut(s) 338, 639
ApoI RAATTY 1 cut(s) 153
Asp718I GGTACC 1 cut(s) 53
AspLEI GCGC 1 cut(s) 515
AsuC2I CCSGG 1 cut(s) 474
AsuHPI GGTGA 1 cut(s) 449
BaeI ACNNNNGTAYC 2 cut(s) 45, 78
BalI TGGCCA 1 cut(s) 579
BanI GGYRCC 1 cut(s) 53
BarI GAAGNNNNNNTAC 2 cut(s) 627, 659
BbsI GAAGAC 2 cut(s) 120, 546
Bbv12I GWGCWC 1 cut(s) 226
BbvI GCAGC 2 cut(s) 325, 626
BccI CCATC 1 cut(s) 112
BceAI ACGGC 2 cut(s) 559, 584
BciT130I CCWGG 3 cut(s) 58, 159, 590
BcnI CCSGG 1 cut(s) 474
BfaI CTAG 2 cut(s) 44, 686
BfoI RGCGCY 1 cut(s) 516
BglI GCCNNNNNGGC 1 cut(s) 585
BisI GCNGC 3 cut(s) 339, 511, 640
BlsI GCNGC 3 cut(s) 340, 512, 641
Bme1390I CCNGG 4 cut(s) 58, 159, 474, 590
BmeRI GACNNNNNGTC 1 cut(s) 524
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 4 cut(s) 58, 159, 474, 590
BmsI GCATC 2 cut(s) 205, 586
BpiI GAAGAC 2 cut(s) 120, 546
BpmI CTGGAG 1 cut(s) 240
BpuEI CTTGAG 2 cut(s) 126, 606
BpuMI CCSGG 1 cut(s) 474
BsaJI CCNNGG 3 cut(s) 57, 589, 600
Bsc4I CCNNNNNNNGG 3 cut(s) 348, 479, 606
Bse118I RCCGGY 1 cut(s) 192
Bse1I ACTGG 1 cut(s) 547
Bse3DI GCAATG 1 cut(s) 333
BseBI CCWGG 3 cut(s) 58, 159, 590
BseDI CCNNGG 3 cut(s) 57, 589, 600
BseGI GGATG 1 cut(s) 87
BseLI CCNNNNNNNGG 3 cut(s) 348, 479, 606
BseMI GCAATG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 547
BseRI GAGGAG 1 cut(s) 69
BseXI GCAGC 2 cut(s) 325, 626
BseYI CCCAGC 2 cut(s) 240, 415
BsgI GTGCAG 1 cut(s) 222
BshFI GGCC 4 cut(s) 192, 546, 579, 588
BshNI GGYRCC 1 cut(s) 53
BsiHKAI GWGCWC 1 cut(s) 226
BsiSI CCGG 3 cut(s) 193, 342, 473
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 3 cut(s) 348, 479, 606
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 4 cut(s) 192, 546, 579, 588
Bsp1286I GDGCHC 1 cut(s) 226
Bsp143I GATC 1 cut(s) 280
BspACI CCGC 1 cut(s) 510
BspANI GGCC 4 cut(s) 192, 546, 579, 588
BspLI GGNNCC 1 cut(s) 55
BspT107I GGYRCC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 333
BsrFI RCCGGY 1 cut(s) 192
BsrI ACTGG 1 cut(s) 547
BssAI RCCGGY 1 cut(s) 192
BssECI CCNNGG 3 cut(s) 57, 589, 600
BssMI GATC 1 cut(s) 280
Bst2UI CCWGG 3 cut(s) 58, 159, 590
Bst4CI ACNGT 3 cut(s) 200, 258, 360
BstC8I GCNNGC 3 cut(s) 194, 488, 492
BstDEI CTNAG 2 cut(s) 287, 456
BstDSI CCRYGG 1 cut(s) 600
BstF5I GGATG 1 cut(s) 87
BstH2I RGCGCY 1 cut(s) 516
BstHHI GCGC 1 cut(s) 515
BstKTI GATC 1 cut(s) 283
BstMBI GATC 1 cut(s) 280
BstMWI GCNNNNNNNGC 3 cut(s) 30, 500, 585
BstNI CCWGG 3 cut(s) 58, 159, 590
BstNSI RCATGY 2 cut(s) 494, 663
BstSCI CCNGG 4 cut(s) 56, 157, 472, 588
BstV1I GCAGC 2 cut(s) 325, 626
BstV2I GAAGAC 2 cut(s) 120, 546
BsuRI GGCC 4 cut(s) 192, 546, 579, 588
BtgI CCRYGG 1 cut(s) 600
BtgZI GCGATG 1 cut(s) 609
BtsCI GGATG 1 cut(s) 87
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 4 cut(s) 205, 224, 263, 356
Cac8I GCNNGC 3 cut(s) 194, 488, 492
CaiI CAGNNNCTG 2 cut(s) 218, 428
CfoI GCGC 1 cut(s) 515
Cfr10I RCCGGY 1 cut(s) 192
CseI GACGC 1 cut(s) 99
Csp6I GTAC 1 cut(s) 54
CviAII CATG 3 cut(s) 83, 491, 660
CviQI GTAC 1 cut(s) 54
DdeI CTNAG 2 cut(s) 287, 456
DpnI GATC 1 cut(s) 282
DpnII GATC 1 cut(s) 280
DraIII CACNNNGTG 1 cut(s) 362
DriI GACNNNNNGTC 1 cut(s) 524
EaeI YGGCCR 2 cut(s) 544, 577
Eam1105I GACNNNNNGTC 1 cut(s) 524
Eco32I GATATC 1 cut(s) 138
Eco57I CTGAAG 1 cut(s) 428
EcoRII CCWGG 3 cut(s) 56, 157, 588
EcoRV GATATC 1 cut(s) 138
EcoT22I ATGCAT 1 cut(s) 391
FaeI CATG 3 cut(s) 86, 494, 663
FaqI GGGAC 1 cut(s) 18
FatI CATG 3 cut(s) 82, 490, 659
Fnu4HI GCNGC 3 cut(s) 339, 511, 640
FokI GGATG 1 cut(s) 74
Fsp4HI GCNGC 3 cut(s) 339, 511, 640
FspBI CTAG 2 cut(s) 44, 686
GlaI GCGC 1 cut(s) 514
GluI GCNGC 3 cut(s) 339, 511, 640
GsaI CCCAGC 2 cut(s) 244, 419
GsuI CTGGAG 1 cut(s) 240
HaeII RGCGCY 1 cut(s) 516
HaeIII GGCC 4 cut(s) 192, 546, 579, 588
HapII CCGG 3 cut(s) 193, 342, 473
HgaI GACGC 1 cut(s) 99
HhaI GCGC 1 cut(s) 515
Hin1II CATG 3 cut(s) 86, 494, 663
Hin6I GCGC 1 cut(s) 513
HinP1I GCGC 1 cut(s) 513
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HinfI GANTC 3 cut(s) 400, 448, 467
HpaII CCGG 3 cut(s) 193, 342, 473
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 316, 447
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 5 cut(s) 29, 112, 166, 627, 629
HpyCH4III ACNGT 3 cut(s) 200, 258, 360
HpyCH4V TGCA 4 cut(s) 203, 389, 494, 659
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 500, 585
HpyF3I CTNAG 2 cut(s) 287, 456
Hsp92II CATG 3 cut(s) 86, 494, 663
HspAI GCGC 1 cut(s) 513
KpnI GGTACC 1 cut(s) 57
KroI GCCGGC 1 cut(s) 192
KroNI GCCGGC 1 cut(s) 194
Kzo9I GATC 1 cut(s) 280
LmnI GCTCC 1 cut(s) 221
Lsp1109I GCAGC 2 cut(s) 325, 626
LweI GCATC 2 cut(s) 205, 586
MaeI CTAG 2 cut(s) 44, 686
MalI GATC 1 cut(s) 282
MboI GATC 1 cut(s) 280
MboII GAAGA 3 cut(s) 66, 120, 551
MhlI GDGCHC 1 cut(s) 226
MlsI TGGCCA 1 cut(s) 579
MluCI AATT 1 cut(s) 153
MluNI TGGCCA 1 cut(s) 579
MlyI GAGTC 1 cut(s) 442
MnlI CCTC 6 cut(s) 23, 90, 182, 389, 427, 514
Mox20I TGGCCA 1 cut(s) 579
Mph1103I ATGCAT 1 cut(s) 391
MroNI GCCGGC 1 cut(s) 192
MscI TGGCCA 1 cut(s) 579
Msp20I TGGCCA 1 cut(s) 579
MspA1I CMGCKG 1 cut(s) 415
MspI CCGG 3 cut(s) 193, 342, 473
MspR9I CCNGG 4 cut(s) 58, 159, 474, 590
MvaI CCWGG 3 cut(s) 58, 159, 590
MwoI GCNNNNNNNGC 3 cut(s) 30, 500, 585
NaeI GCCGGC 1 cut(s) 194
NciI CCSGG 1 cut(s) 474
NdeII GATC 1 cut(s) 280
NgoMIV GCCGGC 1 cut(s) 192
NlaIII CATG 3 cut(s) 86, 494, 663
NlaIV GGNNCC 1 cut(s) 55
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 2 cut(s) 494, 663
PaeI GCATGC 1 cut(s) 494
PdiI GCCGGC 1 cut(s) 194
PfeI GAWTC 2 cut(s) 400, 467
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 3 cut(s) 340, 512, 641
PleI GAGTC 1 cut(s) 442
PpsI GAGTC 1 cut(s) 442
Psp6I CCWGG 3 cut(s) 56, 157, 588
PspFI CCCAGC 2 cut(s) 240, 415
PspGI CCWGG 3 cut(s) 56, 157, 588
PspN4I GGNNCC 1 cut(s) 55
PstNI CAGNNNCTG 2 cut(s) 218, 428
PvuII CAGCTG 1 cut(s) 415
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
SatI GCNGC 3 cut(s) 339, 511, 640
Sau3AI GATC 1 cut(s) 280
SchI GAGTC 1 cut(s) 442
ScrFI CCNGG 4 cut(s) 58, 159, 474, 590
SduI GDGCHC 1 cut(s) 226
SfaNI GCATC 2 cut(s) 205, 586
SfiI GGCCNNNNNGGCC 1 cut(s) 585
SmlI CTYRAG 2 cut(s) 141, 621
SmoI CTYRAG 2 cut(s) 141, 621
SphI GCATGC 1 cut(s) 494
Sse9I AATT 1 cut(s) 153
SsiI CCGC 1 cut(s) 510
SspI AATATT 1 cut(s) 655
SspMI CTAG 2 cut(s) 44, 686
StyD4I CCNGG 4 cut(s) 56, 157, 472, 588
TaaI ACNGT 3 cut(s) 200, 258, 360
TaqI TCGA 1 cut(s) 517
TasI AATT 1 cut(s) 153
TauI GCSGC 1 cut(s) 513
TfiI GAWTC 2 cut(s) 400, 467
TscAI CASTG 4 cut(s) 205, 231, 263, 363
TseI GCWGC 2 cut(s) 338, 639
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 4 cut(s) 205, 231, 263, 363
XapI RAATTY 1 cut(s) 153
XceI RCATGY 2 cut(s) 494, 663
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XspI CTAG 2 cut(s) 44, 686
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.