pycom12g14300

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007631
Physical Location & Seq
Forward (+)
16855298 .. 16855945
648 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g14300.1

Sequence Viewer

Length: 489 bp
ATGCCTCAAGGCTTCAATTTGACTAGTGGGACAGTATCTCATCTTGACAGCAAAAGGGCCAGAGAGGAAATTGCAAAGCGAAGGAAAATTTCATCAGCTAAAGTCGAATCTGAAAGCTCCACAGGTGGTGAAGAATTCTTCAATCCATCCCCTTGTCTTGTAACTCTGGGTCCTGGAGTGAGAATCACCACTGTTGCAGCTGGTGGGCGCCATACTTTAGCATTATCAGATATGGGACAGGTGTGGGGTTGGGGCTATGGAGGCGAAGGACAGCTAGGTTTGGGTACCAGGGTAAAAATGGTTTCTTCTCCTCATGTCATCCCATGTATTGAGCCATCTGCGTCTGGGAAGGATAGGTCTTCTGTGATATCTCAAGGTTCAAAAGTTCCTGGAAGTTTTGTGAAGGAGATTGCTTGTGGAGGCCGGCACAGTGCAGTAATAACAGGTCAGCGTTTTAGGGATCGTATAGAGCTTGCAACCAGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1 RCC1_2

Protein Analysis

163

Amino Acids

17.17

Weight (kDa)

9.6

Isoelectric Point (pI)

57.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 48 - 149 2e-15 RCC1-like domain
RCC1_2 PF13540 62 - 91 2.4e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 79 - 147 3e-07 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 284
AccB1I GGYRCC 2 cut(s) 207, 284
AclWI GGATC 1 cut(s) 468
AcsI RAATTY 2 cut(s) 87, 134
AcyI GRCGYC 1 cut(s) 208
AfaI GTAC 1 cut(s) 286
AgsI TTSAA 3 cut(s) 16, 142, 381
AhlI ACTAGT 1 cut(s) 23
AjnI CCWGG 3 cut(s) 172, 287, 388
AluBI AGCT 5 cut(s) 98, 117, 200, 274, 472
AluI AGCT 5 cut(s) 98, 117, 200, 274, 472
AlwI GGATC 1 cut(s) 468
AoxI GGCC 2 cut(s) 57, 421
ApeKI GCWGC 1 cut(s) 197
ApoI RAATTY 2 cut(s) 87, 134
Asp718I GGTACC 1 cut(s) 284
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 2 cut(s) 57, 170
AsuHPI GGTGA 2 cut(s) 140, 178
AvaII GGWCC 1 cut(s) 170
BaeI ACNNNNGTAYC 2 cut(s) 276, 309
BanI GGYRCC 2 cut(s) 207, 284
BbsI GAAGAC 1 cut(s) 351
BbvI GCAGC 1 cut(s) 209
BccI CCATC 2 cut(s) 154, 343
BciT130I CCWGG 3 cut(s) 174, 289, 390
BcuI ACTAGT 1 cut(s) 23
BfaI CTAG 2 cut(s) 24, 275
BfoI RGCGCY 1 cut(s) 211
BisI GCNGC 1 cut(s) 198
BlsI GCNGC 1 cut(s) 199
Bme1390I CCNGG 3 cut(s) 174, 289, 390
Bme18I GGWCC 1 cut(s) 170
BmgT120I GGNCC 2 cut(s) 57, 170
BmiI GGNNCC 3 cut(s) 171, 209, 286
BmrFI CCNGG 3 cut(s) 174, 289, 390
BpiI GAAGAC 1 cut(s) 351
BpmI CTGGAG 1 cut(s) 195
BpuEI CTTGAG 1 cut(s) 357
BsaHI GRCGYC 1 cut(s) 208
BsaJI CCNNGG 1 cut(s) 288
Bse118I RCCGGY 1 cut(s) 423
BseBI CCWGG 3 cut(s) 174, 289, 390
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 2 cut(s) 146, 318
BseRI GAGGAG 1 cut(s) 300
BseXI GCAGC 1 cut(s) 209
BsgI GTGCAG 1 cut(s) 453
BshFI GGCC 2 cut(s) 59, 423
BshNI GGYRCC 2 cut(s) 207, 284
BsiSI CCGG 1 cut(s) 424
BslFI GGGAC 2 cut(s) 43, 249
BsmFI GGGAC 2 cut(s) 43, 249
BsnI GGCC 2 cut(s) 59, 423
Bsp143I GATC 1 cut(s) 460
BspANI GGCC 2 cut(s) 59, 423
BspLI GGNNCC 3 cut(s) 171, 209, 286
BspPI GGATC 1 cut(s) 468
BspT107I GGYRCC 2 cut(s) 207, 284
BsrFI RCCGGY 1 cut(s) 423
BssAI RCCGGY 1 cut(s) 423
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 1 cut(s) 460
BssNI GRCGYC 1 cut(s) 208
Bst2UI CCWGG 3 cut(s) 174, 289, 390
Bst4CI ACNGT 3 cut(s) 34, 193, 431
BstACI GRCGYC 1 cut(s) 208
BstC8I GCNNGC 2 cut(s) 425, 474
BstF5I GGATG 2 cut(s) 146, 318
BstH2I RGCGCY 1 cut(s) 211
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 1 cut(s) 463
BstMBI GATC 1 cut(s) 460
BstMWI GCNNNNNNNGC 1 cut(s) 261
BstNI CCWGG 3 cut(s) 174, 289, 390
BstSCI CCNGG 3 cut(s) 172, 287, 388
BstV1I GCAGC 1 cut(s) 209
BstV2I GAAGAC 1 cut(s) 351
BsuRI GGCC 2 cut(s) 59, 423
BtsCI GGATG 2 cut(s) 146, 318
BtsIMutI CAGTG 2 cut(s) 189, 436
Cac8I GCNNGC 2 cut(s) 425, 474
CfoI GCGC 1 cut(s) 210
Cfr10I RCCGGY 1 cut(s) 423
Cfr13I GGNCC 2 cut(s) 57, 170
CseI GACGC 1 cut(s) 330
Csp6I GTAC 1 cut(s) 285
CviAII CATG 2 cut(s) 314, 324
CviQI GTAC 1 cut(s) 285
DinI GGCGCC 1 cut(s) 209
DpnI GATC 1 cut(s) 462
DpnII GATC 1 cut(s) 460
Eco32I GATATC 1 cut(s) 369
Eco47I GGWCC 1 cut(s) 170
EcoO109I RGGNCCY 1 cut(s) 170
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 3 cut(s) 172, 287, 388
EcoRV GATATC 1 cut(s) 369
EgeI GGCGCC 1 cut(s) 209
EheI GGCGCC 1 cut(s) 209
FaeI CATG 2 cut(s) 317, 327
FaiI YATR 6 cut(s) 213, 233, 258, 315, 325, 467
FaqI GGGAC 2 cut(s) 43, 249
FatI CATG 2 cut(s) 313, 323
Fnu4HI GCNGC 1 cut(s) 198
FokI GGATG 2 cut(s) 133, 305
Fsp4HI GCNGC 1 cut(s) 198
FspBI CTAG 2 cut(s) 24, 275
GlaI GCGC 1 cut(s) 209
GluI GCNGC 1 cut(s) 198
GsuI CTGGAG 1 cut(s) 195
HaeII RGCGCY 1 cut(s) 211
HaeIII GGCC 2 cut(s) 59, 423
HapII CCGG 1 cut(s) 424
HgaI GACGC 1 cut(s) 330
HhaI GCGC 1 cut(s) 210
Hin1I GRCGYC 1 cut(s) 208
Hin1II CATG 2 cut(s) 317, 327
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HinfI GANTC 2 cut(s) 107, 183
HpaII CCGG 1 cut(s) 424
HphI GGTGA 2 cut(s) 140, 178
Hpy188I TCNGA 2 cut(s) 112, 229
Hpy188III TCNNGA 1 cut(s) 44
HpyAV CCTTC 4 cut(s) 75, 260, 343, 397
HpyCH4III ACNGT 3 cut(s) 34, 193, 431
HpyCH4V TGCA 4 cut(s) 74, 197, 434, 476
HpyF10VI GCNNNNNNNGC 1 cut(s) 261
Hsp92I GRCGYC 1 cut(s) 208
Hsp92II CATG 2 cut(s) 317, 327
HspAI GCGC 1 cut(s) 208
KasI GGCGCC 1 cut(s) 207
KpnI GGTACC 1 cut(s) 288
KroI GCCGGC 1 cut(s) 423
KroNI GCCGGC 1 cut(s) 425
Kzo9I GATC 1 cut(s) 460
LmnI GCTCC 1 cut(s) 122
Lsp1109I GCAGC 1 cut(s) 209
MaeI CTAG 2 cut(s) 24, 275
MaeIII GTNAC 1 cut(s) 160
MalI GATC 1 cut(s) 462
MboI GATC 1 cut(s) 460
MboII GAAGA 4 cut(s) 130, 143, 297, 351
MluCI AATT 4 cut(s) 16, 69, 87, 134
Mly113I GGCGCC 1 cut(s) 208
MnlI CCTC 5 cut(s) 15, 58, 254, 321, 413
MroNI GCCGGC 1 cut(s) 423
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 1 cut(s) 424
MspR9I CCNGG 3 cut(s) 174, 289, 390
MvaI CCWGG 3 cut(s) 174, 289, 390
MwoI GCNNNNNNNGC 1 cut(s) 261
NaeI GCCGGC 1 cut(s) 425
NarI GGCGCC 1 cut(s) 208
NdeII GATC 1 cut(s) 460
NgoMIV GCCGGC 1 cut(s) 423
NlaIII CATG 2 cut(s) 317, 327
NlaIV GGNNCC 3 cut(s) 171, 209, 286
PdiI GCCGGC 1 cut(s) 425
PfeI GAWTC 2 cut(s) 107, 183
PfoI TCCNGGA 2 cut(s) 172, 388
PkrI GCNGC 1 cut(s) 199
PluTI GGCGCC 1 cut(s) 211
PpuMI RGGWCCY 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 170
Psp6I CCWGG 3 cut(s) 172, 287, 388
PspGI CCWGG 3 cut(s) 172, 287, 388
PspN4I GGNNCC 3 cut(s) 171, 209, 286
PspPI GGNCC 2 cut(s) 57, 170
PspPPI RGGWCCY 1 cut(s) 170
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 1 cut(s) 286
RsaNI GTAC 1 cut(s) 285
SatI GCNGC 1 cut(s) 198
Sau3AI GATC 1 cut(s) 460
Sau96I GGNCC 2 cut(s) 57, 170
ScrFI CCNGG 3 cut(s) 174, 289, 390
SfoI GGCGCC 1 cut(s) 209
SinI GGWCC 1 cut(s) 170
SmlI CTYRAG 2 cut(s) 6, 372
SmoI CTYRAG 2 cut(s) 6, 372
SpeI ACTAGT 1 cut(s) 23
Sse9I AATT 4 cut(s) 16, 69, 87, 134
SspDI GGCGCC 1 cut(s) 207
SspMI CTAG 2 cut(s) 24, 275
StyD4I CCNGG 3 cut(s) 172, 287, 388
TaaI ACNGT 3 cut(s) 34, 193, 431
TaqI TCGA 1 cut(s) 105
TasI AATT 4 cut(s) 16, 69, 87, 134
TfiI GAWTC 2 cut(s) 107, 183
TscAI CASTG 2 cut(s) 196, 436
TseI GCWGC 1 cut(s) 197
TspDTI ATGAA 1 cut(s) 81
TspRI CASTG 2 cut(s) 196, 436
VpaK11BI GGWCC 1 cut(s) 170
XapI RAATTY 2 cut(s) 87, 134
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XspI CTAG 2 cut(s) 24, 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.