MD04G1137600.v1.1

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016161
Physical Location & Seq
Forward (+)
22513152 .. 22513916
765 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1137600.v1.1.491

Sequence Viewer

Length: 765 bp
ATGACCAAGATCGACACCCTCCGTCGATTTCTCCTCCCCTGCTTGTCCCCCTCCTCCGCCAAATCCACCCACTCCGCCGCCGCCGCCCCCAAGAAGCGCCTCAGCACCTCCTTGCGCGACGACCTGGACGACATCAACGCCGCCGCCACCAAAAGCTTAGACCCCAAGGACCAAGATTCACAATCTTCCACCTCGCCCACCCAAAACGACGGCGTGGCGATTCCGGCCCAGCAGACCCGCCCCTCCAAATCCATGGTCATCGGCACAATCTTCGGCCACCGCCGCGGCCACGTGTGGTTGTGCATCCAGCACGACCGCCTCTCCACCAAACCCACCCTCCTCCTCGAGCTCTCGGTCTCGACCTACCAGCTAGTCAACGAGATGCGATTCGGGCTCGTCCGCGTGACCCTCGAGTGCGACGAGTCGGACCGGCCCCAGCTCGCTGATTGCCCGCTCCGATCCGTCCCCATTTGGACCGTTCACTGCAACGGCCGCAAACTCGGGTCCGCGGCAAGAAGAAAAGCGAGCGAGAAGGTGAAATTGATGCTGAAGATGATGCAGTCGACGACGGTCGGAGCCGGGGTAATGCCGGCAGGGTTCGCGCTCGGGTCGGAGGCGGAGTCGGGTCAGGAAGTAATGTACATGAGGGCGAATTATGAGCACGTGGTGGGGAGCGCTGACTCGGAGTCGTTTCATCTGATTAACCCGGATCAGTGCCCGGGTCAAGAACTCAGTGTCTTCCTTCTCCGGTCCAGGCACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

255

Amino Acids

27.86

Weight (kDa)

8.93

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 86 - 250 9.2e-60 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016161)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37880
fragaria_vesca FvH4_6g12850
malus_domestica MD04G1137600.v1.1 MD12G1152400.v1.1
prunus_persica Prupe.6G263600_v2.0.a1
pyrus_communis pycom04g12600
rosa_chinensis RchiOBHm_Chr3g0464851
rosa_laevigata RLG00000024669
rosa_multiflora Rmu_sc0007114.1_g000004
rosa_roxburghii Rroxscaffold_6G00415390
rosa_rugosa Rorug03G0074400
rosa_samantha Rh3BG137200 Rh3CG139800 Rh3DG138600
rosa_wichuraiana Rw3G011210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 454
AccI GTMKAC 1 cut(s) 563
AccII CGCG 5 cut(s) 117, 285, 402, 509, 602
AclWI GGATC 2 cut(s) 453, 717
AcoI YGGCCR 3 cut(s) 274, 286, 490
AcuI CTGAAG 1 cut(s) 569
AcvI CACGTG 2 cut(s) 292, 664
AdeI CACNNNGTG 1 cut(s) 667
AfaI GTAC 1 cut(s) 641
AfeI AGCGCT 1 cut(s) 676
AflIII ACRYGT 1 cut(s) 291
AhdI GACNNNNNGTC 1 cut(s) 685
AjnI CCWGG 2 cut(s) 123, 752
AluBI AGCT 4 cut(s) 156, 349, 370, 439
AluI AGCT 4 cut(s) 156, 349, 370, 439
Alw21I GWGCWC 2 cut(s) 351, 663
Alw26I GTCTC 1 cut(s) 361
AlwI GGATC 2 cut(s) 453, 717
Ama87I CYCGRG 5 cut(s) 344, 410, 500, 605, 718
Aor51HI AGCGCT 1 cut(s) 676
AoxI GGCC 5 cut(s) 225, 274, 286, 431, 490
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
AspLEI GCGC 4 cut(s) 99, 117, 604, 677
AspS9I GGNCC 7 cut(s) 169, 226, 427, 432, 474, 504, 750
AsuC2I CCSGG 4 cut(s) 580, 707, 719, 720
AsuHPI GGTGA 1 cut(s) 547
AvaI CYCGRG 5 cut(s) 344, 410, 500, 605, 718
AvaII GGWCC 5 cut(s) 169, 427, 474, 504, 750
BaeGI GKGCMC 1 cut(s) 719
BanII GRGCYC 2 cut(s) 351, 396
BbrPI CACGTG 2 cut(s) 292, 664
BbsI GAAGAC 1 cut(s) 730
Bbv12I GWGCWC 2 cut(s) 351, 663
BbvCI CCTCAGC 1 cut(s) 101
BceAI ACGGC 2 cut(s) 226, 505
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BciT130I CCWGG 2 cut(s) 125, 754
BcnI CCSGG 4 cut(s) 580, 707, 719, 720
BcoDI GTCTC 1 cut(s) 361
BfaI CTAG 1 cut(s) 371
BfoI RGCGCY 2 cut(s) 100, 678
BisI GCNGC 9 cut(s) 78, 81, 84, 141, 144, 283, 286, 493, 510
BlsI GCNGC 9 cut(s) 79, 82, 85, 142, 145, 284, 287, 494, 511
Bme1390I CCNGG 6 cut(s) 125, 580, 707, 719, 720, 754
Bme18I GGWCC 5 cut(s) 169, 427, 474, 504, 750
BmeRI GACNNNNNGTC 1 cut(s) 685
BmeT110I CYCGRG 5 cut(s) 344, 410, 500, 605, 718
BmgT120I GGNCC 7 cut(s) 169, 226, 427, 432, 474, 504, 750
BmiI GGNNCC 3 cut(s) 434, 505, 577
BmrFI CCNGG 6 cut(s) 125, 580, 707, 719, 720, 754
BmsI GCATC 4 cut(s) 312, 372, 534, 546
BoxI GACNNNNGTC 1 cut(s) 569
BpiI GAAGAC 1 cut(s) 730
Bpu10I CCTNAGC 1 cut(s) 101
BpuMI CCSGG 4 cut(s) 580, 707, 719, 720
BsaAI YACGTR 2 cut(s) 292, 664
BsaI GGTCTC 1 cut(s) 361
BsaJI CCNNGG 6 cut(s) 165, 252, 283, 507, 579, 718
BsaWI WCCGGW 1 cut(s) 747
BsaXI ACNNNNNCTCC 8 cut(s) 305, 321, 335, 351, 567, 597, 605, 635
Bse118I RCCGGY 2 cut(s) 429, 589
BseBI CCWGG 2 cut(s) 125, 754
BseDI CCNNGG 6 cut(s) 165, 252, 283, 507, 579, 718
BseGI GGATG 1 cut(s) 303
BseMII CTCAG 2 cut(s) 115, 745
BseRI GAGGAG 4 cut(s) 23, 43, 329, 332
BseSI GKGCMC 1 cut(s) 719
BseX3I CGGCCG 1 cut(s) 490
BseYI CCCAGC 2 cut(s) 228, 435
Bsh1236I CGCG 5 cut(s) 117, 285, 402, 509, 602
Bsh1285I CGRYCG 3 cut(s) 316, 493, 573
BshFI GGCC 5 cut(s) 227, 276, 288, 433, 492
BsiEI CGRYCG 3 cut(s) 316, 493, 573
BsiHKAI GWGCWC 2 cut(s) 351, 663
BsiHKCI CYCGRG 5 cut(s) 344, 410, 500, 605, 718
BsiSI CCGG 7 cut(s) 224, 430, 579, 590, 707, 719, 748
BslFI GGGAC 2 cut(s) 31, 449
BsmAI GTCTC 1 cut(s) 361
BsmFI GGGAC 2 cut(s) 31, 449
BsnI GGCC 5 cut(s) 227, 276, 288, 433, 492
Bso31I GGTCTC 1 cut(s) 361
BsoBI CYCGRG 5 cut(s) 344, 410, 500, 605, 718
Bsp1286I GDGCHC 4 cut(s) 351, 396, 663, 719
Bsp1407I TGTACA 1 cut(s) 639
Bsp143I GATC 3 cut(s) 9, 458, 709
Bsp19I CCATGG 1 cut(s) 252
BspANI GGCC 5 cut(s) 227, 276, 288, 433, 492
BspCNI CTCAG 2 cut(s) 114, 744
BspFNI CGCG 5 cut(s) 117, 285, 402, 509, 602
BspLI GGNNCC 3 cut(s) 434, 505, 577
BspPI GGATC 2 cut(s) 453, 717
BspTNI GGTCTC 1 cut(s) 361
BsrBI CCGCTC 1 cut(s) 454
BsrFI RCCGGY 2 cut(s) 429, 589
BsrGI TGTACA 1 cut(s) 639
BssAI RCCGGY 2 cut(s) 429, 589
BssECI CCNNGG 6 cut(s) 165, 252, 283, 507, 579, 718
BssMI GATC 3 cut(s) 9, 458, 709
BssT1I CCWWGG 2 cut(s) 165, 252
Bst2UI CCWGG 2 cut(s) 125, 754
Bst4CI ACNGT 2 cut(s) 478, 571
BstAUI TGTACA 1 cut(s) 639
BstBAI YACGTR 2 cut(s) 292, 664
BstC8I GCNNGC 4 cut(s) 441, 452, 526, 591
BstDEI CTNAG 3 cut(s) 101, 157, 731
BstDSI CCRYGG 3 cut(s) 252, 283, 507
BstF5I GGATG 1 cut(s) 303
BstFNI CGCG 5 cut(s) 117, 285, 402, 509, 602
BstH2I RGCGCY 2 cut(s) 100, 678
BstHHI GCGC 4 cut(s) 99, 117, 604, 677
BstKTI GATC 3 cut(s) 12, 461, 712
BstMAI GTCTC 1 cut(s) 361
BstMBI GATC 3 cut(s) 9, 458, 709
BstMCI CGRYCG 3 cut(s) 316, 493, 573
BstMWI GCNNNNNNNGC 6 cut(s) 83, 224, 282, 391, 492, 599
BstNI CCWGG 2 cut(s) 125, 754
BstPAI GACNNNNGTC 1 cut(s) 569
BstSCI CCNGG 6 cut(s) 123, 578, 705, 717, 718, 752
BstSLI GKGCMC 1 cut(s) 719
BstUI CGCG 5 cut(s) 117, 285, 402, 509, 602
BstV2I GAAGAC 1 cut(s) 730
BstXI CCANNNNNNTGG 1 cut(s) 253
BstZI CGGCCG 1 cut(s) 490
BsuRI GGCC 5 cut(s) 227, 276, 288, 433, 492
BtgI CCRYGG 3 cut(s) 252, 283, 507
BtsCI GGATG 1 cut(s) 303
BtsI GCAGTG 1 cut(s) 481
BtsIMutI CAGTG 3 cut(s) 481, 719, 739
Cac8I GCNNGC 4 cut(s) 441, 452, 526, 591
CfoI GCGC 4 cut(s) 99, 117, 604, 677
Cfr10I RCCGGY 2 cut(s) 429, 589
Cfr13I GGNCC 7 cut(s) 169, 226, 427, 432, 474, 504, 750
Cfr42I CCGCGG 2 cut(s) 286, 510
Cfr9I CCCGGG 1 cut(s) 718
CpoI CGGWCCG 1 cut(s) 427
Csp6I GTAC 1 cut(s) 640
CspI CGGWCCG 1 cut(s) 427
CviAII CATG 2 cut(s) 253, 643
CviQI GTAC 1 cut(s) 640
DdeI CTNAG 3 cut(s) 101, 157, 731
DpnI GATC 3 cut(s) 11, 460, 711
DpnII GATC 3 cut(s) 9, 458, 709
DraIII CACNNNGTG 1 cut(s) 667
DriI GACNNNNNGTC 1 cut(s) 685
EaeI YGGCCR 3 cut(s) 274, 286, 490
EagI CGGCCG 1 cut(s) 490
Eam1105I GACNNNNNGTC 1 cut(s) 685
EciI GGCGGA 3 cut(s) 46, 64, 632
Ecl136II GAGCTC 1 cut(s) 349
EclXI CGGCCG 1 cut(s) 490
Eco130I CCWWGG 2 cut(s) 165, 252
Eco24I GRGCYC 2 cut(s) 351, 396
Eco31I GGTCTC 1 cut(s) 361
Eco47I GGWCC 5 cut(s) 169, 427, 474, 504, 750
Eco47III AGCGCT 1 cut(s) 676
Eco52I CGGCCG 1 cut(s) 490
Eco53kI GAGCTC 1 cut(s) 349
Eco57I CTGAAG 1 cut(s) 569
Eco72I CACGTG 2 cut(s) 292, 664
Eco88I CYCGRG 5 cut(s) 344, 410, 500, 605, 718
EcoICRI GAGCTC 1 cut(s) 349
EcoRII CCWGG 2 cut(s) 123, 752
EcoT14I CCWWGG 2 cut(s) 165, 252
EcoT38I GRGCYC 2 cut(s) 351, 396
ErhI CCWWGG 2 cut(s) 165, 252
FaeI CATG 2 cut(s) 256, 646
FaiI YATR 3 cut(s) 254, 644, 657
FaqI GGGAC 2 cut(s) 31, 449
FatI CATG 2 cut(s) 252, 642
FauI CCCGC 2 cut(s) 245, 459
FblI GTMKAC 1 cut(s) 563
Fnu4HI GCNGC 9 cut(s) 78, 81, 84, 141, 144, 283, 286, 493, 510
FokI GGATG 1 cut(s) 290
FriOI GRGCYC 2 cut(s) 351, 396
Fsp4HI GCNGC 9 cut(s) 78, 81, 84, 141, 144, 283, 286, 493, 510
FspBI CTAG 1 cut(s) 371
GlaI GCGC 4 cut(s) 98, 116, 603, 676
GluI GCNGC 9 cut(s) 78, 81, 84, 141, 144, 283, 286, 493, 510
GsaI CCCAGC 2 cut(s) 232, 439
HaeII RGCGCY 2 cut(s) 100, 678
HaeIII GGCC 5 cut(s) 227, 276, 288, 433, 492
HapII CCGG 7 cut(s) 224, 430, 579, 590, 707, 719, 748
HhaI GCGC 4 cut(s) 99, 117, 604, 677
Hin1II CATG 2 cut(s) 256, 646
Hin6I GCGC 4 cut(s) 97, 115, 602, 675
HinP1I GCGC 4 cut(s) 97, 115, 602, 675
HincII GTYRAC 2 cut(s) 376, 564
HindII GTYRAC 2 cut(s) 376, 564
HindIII AAGCTT 1 cut(s) 154
HinfI GANTC 7 cut(s) 176, 220, 387, 422, 620, 680, 686
HpaII CCGG 7 cut(s) 224, 430, 579, 590, 707, 719, 748
HphI GGTGA 1 cut(s) 547
Hpy166II GTNNAC 3 cut(s) 376, 481, 564
Hpy188I TCNGA 6 cut(s) 427, 458, 575, 613, 685, 699
Hpy188III TCNNGA 4 cut(s) 358, 629, 725, 762
Hpy8I GTNNAC 3 cut(s) 376, 481, 564
Hpy99I CGWCG 6 cut(s) 27, 122, 212, 422, 568, 571
HpyAV CCTTC 2 cut(s) 526, 752
HpyCH4III ACNGT 2 cut(s) 478, 571
HpyCH4IV ACGT 2 cut(s) 291, 663
HpyCH4V TGCA 3 cut(s) 303, 486, 559
HpyF10VI GCNNNNNNNGC 6 cut(s) 83, 224, 282, 391, 492, 599
HpyF3I CTNAG 3 cut(s) 101, 157, 731
HpySE526I ACGT 2 cut(s) 291, 663
Hsp92II CATG 2 cut(s) 256, 646
HspAI GCGC 4 cut(s) 97, 115, 602, 675
KroI GCCGGC 1 cut(s) 589
KroNI GCCGGC 1 cut(s) 591
KspI CCGCGG 2 cut(s) 286, 510
Kzo9I GATC 3 cut(s) 9, 458, 709
LmnI GCTCC 3 cut(s) 459, 575, 672
LweI GCATC 4 cut(s) 312, 372, 534, 546
MaeI CTAG 1 cut(s) 371
MaeII ACGT 2 cut(s) 291, 663
MaeIII GTNAC 1 cut(s) 403
MalI GATC 3 cut(s) 11, 460, 711
MbiI CCGCTC 1 cut(s) 454
MboI GATC 3 cut(s) 9, 458, 709
MboII GAAGA 5 cut(s) 177, 262, 528, 562, 730
MhlI GDGCHC 4 cut(s) 351, 396, 663, 719
MluCI AATT 2 cut(s) 539, 652
MlyI GAGTC 4 cut(s) 431, 629, 674, 695
MmeI TCCRAC 3 cut(s) 405, 553, 591
MroNI GCCGGC 1 cut(s) 589
MseI TTAA 1 cut(s) 702
MspA1I CMGCKG 2 cut(s) 285, 509
MspI CCGG 7 cut(s) 224, 430, 579, 590, 707, 719, 748
MspR9I CCNGG 6 cut(s) 125, 580, 707, 719, 720, 754
MvaI CCWGG 2 cut(s) 125, 754
MvnI CGCG 5 cut(s) 117, 285, 402, 509, 602
MwoI GCNNNNNNNGC 6 cut(s) 83, 224, 282, 391, 492, 599
NaeI GCCGGC 1 cut(s) 591
NciI CCSGG 4 cut(s) 580, 707, 719, 720
NcoI CCATGG 1 cut(s) 252
NdeII GATC 3 cut(s) 9, 458, 709
NgoMIV GCCGGC 1 cut(s) 589
NlaIII CATG 2 cut(s) 256, 646
NlaIV GGNNCC 3 cut(s) 434, 505, 577
NmuCI GTSAC 1 cut(s) 403
PaeR7I CTCGAG 2 cut(s) 344, 410
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 417
PdiI GCCGGC 1 cut(s) 591
PfeI GAWTC 3 cut(s) 176, 220, 387
PkrI GCNGC 9 cut(s) 79, 82, 85, 142, 145, 284, 287, 494, 511
PleI GAGTC 4 cut(s) 430, 628, 674, 694
PmaCI CACGTG 2 cut(s) 292, 664
PmlI CACGTG 2 cut(s) 292, 664
PpsI GAGTC 4 cut(s) 430, 628, 674, 694
Ppu21I YACGTR 2 cut(s) 292, 664
PshAI GACNNNNGTC 1 cut(s) 569
Psp124BI GAGCTC 1 cut(s) 351
Psp6I CCWGG 2 cut(s) 123, 752
PspCI CACGTG 2 cut(s) 292, 664
PspFI CCCAGC 2 cut(s) 228, 435
PspGI CCWGG 2 cut(s) 123, 752
PspN4I GGNNCC 3 cut(s) 434, 505, 577
PspPI GGNCC 7 cut(s) 169, 226, 427, 432, 474, 504, 750
PspXI VCTCGAGB 2 cut(s) 344, 410
RsaI GTAC 1 cut(s) 641
RsaNI GTAC 1 cut(s) 640
Rsr2I CGGWCCG 1 cut(s) 427
RsrII CGGWCCG 1 cut(s) 427
SacI GAGCTC 1 cut(s) 351
SacII CCGCGG 2 cut(s) 286, 510
SalI GTCGAC 1 cut(s) 562
SaqAI TTAA 1 cut(s) 702
SatI GCNGC 9 cut(s) 78, 81, 84, 141, 144, 283, 286, 493, 510
Sau3AI GATC 3 cut(s) 9, 458, 709
Sau96I GGNCC 7 cut(s) 169, 226, 427, 432, 474, 504, 750
SchI GAGTC 4 cut(s) 431, 629, 674, 695
ScrFI CCNGG 6 cut(s) 125, 580, 707, 719, 720, 754
SduI GDGCHC 4 cut(s) 351, 396, 663, 719
SfaNI GCATC 4 cut(s) 312, 372, 534, 546
Sfr274I CTCGAG 2 cut(s) 344, 410
Sfr303I CCGCGG 2 cut(s) 286, 510
SgrBI CCGCGG 2 cut(s) 286, 510
SinI GGWCC 5 cut(s) 169, 427, 474, 504, 750
SlaI CTCGAG 2 cut(s) 344, 410
SmaI CCCGGG 1 cut(s) 720
SmlI CTYRAG 2 cut(s) 344, 410
SmoI CTYRAG 2 cut(s) 344, 410
Sse9I AATT 2 cut(s) 539, 652
SspMI CTAG 1 cut(s) 371
SstI GAGCTC 1 cut(s) 351
StyD4I CCNGG 6 cut(s) 123, 578, 705, 717, 718, 752
StyI CCWWGG 2 cut(s) 165, 252
TaaI ACNGT 2 cut(s) 478, 571
TaiI ACGT 2 cut(s) 294, 666
TaqI TCGA 6 cut(s) 12, 25, 345, 359, 411, 563
TaqII GACCGA 1 cut(s) 343
TasI AATT 2 cut(s) 539, 652
TatI WGTACW 1 cut(s) 639
TauI GCSGC 9 cut(s) 80, 83, 86, 143, 146, 285, 288, 495, 512
TfiI GAWTC 3 cut(s) 176, 220, 387
Tru1I TTAA 1 cut(s) 702
Tru9I TTAA 1 cut(s) 702
TscAI CASTG 3 cut(s) 488, 719, 739
TseFI GTSAC 1 cut(s) 403
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 1 cut(s) 683
TspGWI ACGGA 2 cut(s) 11, 451
TspMI CCCGGG 1 cut(s) 718
TspRI CASTG 3 cut(s) 488, 719, 739
VpaK11BI GGWCC 5 cut(s) 169, 427, 474, 504, 750
XhoI CTCGAG 2 cut(s) 344, 410
XmaI CCCGGG 1 cut(s) 718
XmiI GTMKAC 1 cut(s) 563
XspI CTAG 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.