MD12G1152400.v1.1

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016161
Physical Location & Seq
Forward (+)
23259281 .. 23260051
771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1152400.v1.1.491

Sequence Viewer

Length: 771 bp
ATGACCAAGATCAACGCCCTCCATCGATTTCTTCTCCCCTGCTTCTCCCCTACCTCCGCGAAATCCACCCGCTCCGCCACCGCCGCCCCCAAGAAACGCCTCAGCACCTCCTTGCGCAACGACCTGGACGACATCAACGCCGCCGCCGCCAAAGCCCTAGATCCCCAGGACCAAGATTCCCAATCTTCCACCTCGCCCACCCCCCAAAACGAAGTCGTCTCGGTGGTTCTGCCCCAGCAGCCCCGCCCCTCGAAATCCATGGTCATCGGCACAATCTTCGGCCACCGCCGCGGGCACGTGTGGTTGTGCATCCAGCACGACCGCCGCTCCACCAAACCCACCCTCCTCCTCGAGCTCTCGGTCTCGACCCACCAGCTCGTCAACGAGATGCAATTGGGGCTCGTCCGCGTGACACTCGAGTGCAACGAGTCGGACCGGCCCCAGCTCATTGATTGCTCGCTCCTGACCGTTCCCATTTGGACCGTTCACATCAACGGCCGCAAATTCGGGTTCGCGGCGAGAAGAAAAGCGAGCGAGAAGGTGAAATTGATGCTGAAGCTGATGCAGTCGATGACGGTCGGAGCCGGGGTAATGCCGGCTGGGTTCGCGCTTGGGTCGGAGGCGGAGTCGCATCAGGAGGTGATGTACATGAGGGCGAATTACGATCACGTGGTGGGAAGCGCTGACTCGGAGTCGTTTCATCTGATTAACCCGGATCAGTGTCCGAGTCAAGAACTCAGTGTCTTCCTGCTCCGGTCCAGGCACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

257

Amino Acids

28.32

Weight (kDa)

9.2

Isoelectric Point (pI)

57.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 88 - 252 1.8e-60 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016161)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37880
fragaria_vesca FvH4_6g12850
malus_domestica MD04G1137600.v1.1 MD12G1152400.v1.1
prunus_persica Prupe.6G263600_v2.0.a1
pyrus_communis pycom04g12600
rosa_chinensis RchiOBHm_Chr3g0464851
rosa_laevigata RLG00000024669
rosa_multiflora Rmu_sc0007114.1_g000004
rosa_roxburghii Rroxscaffold_6G00415390
rosa_rugosa Rorug03G0074400
rosa_samantha Rh3BG137200 Rh3CG139800 Rh3DG138600
rosa_wichuraiana Rw3G011210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 116
AccBSI CCGCTC 2 cut(s) 72, 327
AccII CGCG 5 cut(s) 59, 291, 408, 515, 608
AclWI GGATC 2 cut(s) 155, 723
AcoI YGGCCR 2 cut(s) 280, 496
AcsI RAATTY 1 cut(s) 503
AcuI CTGAAG 1 cut(s) 575
AcvI CACGTG 2 cut(s) 298, 670
AdeI CACNNNGTG 1 cut(s) 673
AfaI GTAC 1 cut(s) 647
AfeI AGCGCT 1 cut(s) 682
AflIII ACRYGT 1 cut(s) 297
AhdI GACNNNNNGTC 1 cut(s) 691
AjnI CCWGG 3 cut(s) 123, 165, 758
AleI CACNNNNGTG 1 cut(s) 418
AluBI AGCT 4 cut(s) 355, 376, 445, 559
AluI AGCT 4 cut(s) 355, 376, 445, 559
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 2 cut(s) 223, 367
AlwI GGATC 2 cut(s) 155, 723
Ama87I CYCGRG 2 cut(s) 350, 416
Aor51HI AGCGCT 1 cut(s) 682
AoxI GGCC 3 cut(s) 280, 437, 496
ApeKI GCWGC 1 cut(s) 238
ApoI RAATTY 1 cut(s) 503
AspLEI GCGC 3 cut(s) 117, 610, 683
AspS9I GGNCC 5 cut(s) 169, 433, 438, 480, 756
AsuC2I CCSGG 2 cut(s) 586, 713
AsuHPI GGTGA 2 cut(s) 553, 652
AvaI CYCGRG 2 cut(s) 350, 416
AvaII GGWCC 4 cut(s) 169, 433, 480, 756
BaeGI GKGCMC 1 cut(s) 297
BanII GRGCYC 2 cut(s) 357, 402
BbrPI CACGTG 2 cut(s) 298, 670
BbsI GAAGAC 1 cut(s) 736
Bbv12I GWGCWC 1 cut(s) 357
BbvCI CCTCAGC 1 cut(s) 101
BbvI GCAGC 1 cut(s) 250
BccI CCATC 1 cut(s) 30
BceAI ACGGC 1 cut(s) 511
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BciT130I CCWGG 3 cut(s) 125, 167, 760
BcnI CCSGG 2 cut(s) 586, 713
BcoDI GTCTC 2 cut(s) 223, 367
BfaI CTAG 1 cut(s) 158
BfoI RGCGCY 1 cut(s) 684
BisI GCNGC 9 cut(s) 84, 141, 144, 147, 239, 289, 325, 499, 516
BlsI GCNGC 9 cut(s) 85, 142, 145, 148, 240, 290, 326, 500, 517
Bme1390I CCNGG 5 cut(s) 125, 167, 586, 713, 760
Bme18I GGWCC 4 cut(s) 169, 433, 480, 756
BmeRI GACNNNNNGTC 1 cut(s) 691
BmeT110I CYCGRG 2 cut(s) 350, 416
BmgT120I GGNCC 5 cut(s) 169, 433, 438, 480, 756
BmiI GGNNCC 2 cut(s) 440, 583
BmrFI CCNGG 5 cut(s) 125, 167, 586, 713, 760
BmsI GCATC 5 cut(s) 318, 378, 540, 552, 640
BpiI GAAGAC 1 cut(s) 736
Bpu10I CCTNAGC 1 cut(s) 101
BpuMI CCSGG 2 cut(s) 586, 713
Bsa29I ATCGAT 1 cut(s) 25
BsaAI YACGTR 2 cut(s) 298, 670
BsaI GGTCTC 1 cut(s) 367
BsaJI CCNNGG 4 cut(s) 165, 258, 289, 585
BsaWI WCCGGW 1 cut(s) 753
Bse118I RCCGGY 2 cut(s) 435, 595
BseBI CCWGG 3 cut(s) 125, 167, 760
BseCI ATCGAT 1 cut(s) 25
BseDI CCNNGG 4 cut(s) 165, 258, 289, 585
BseGI GGATG 1 cut(s) 309
BseMII CTCAG 2 cut(s) 115, 751
BseRI GAGGAG 2 cut(s) 335, 338
BseSI GKGCMC 1 cut(s) 297
BseX3I CGGCCG 1 cut(s) 496
BseXI GCAGC 1 cut(s) 250
BseYI CCCAGC 3 cut(s) 234, 441, 599
Bsh1236I CGCG 5 cut(s) 59, 291, 408, 515, 608
Bsh1285I CGRYCG 3 cut(s) 322, 499, 579
BshFI GGCC 3 cut(s) 282, 439, 498
BshVI ATCGAT 1 cut(s) 25
BsiEI CGRYCG 3 cut(s) 322, 499, 579
BsiHKAI GWGCWC 1 cut(s) 357
BsiHKCI CYCGRG 2 cut(s) 350, 416
BsiSI CCGG 5 cut(s) 436, 585, 596, 713, 754
BsmAI GTCTC 2 cut(s) 223, 367
BsmBI CGTCTC 1 cut(s) 223
BsnI GGCC 3 cut(s) 282, 439, 498
Bso31I GGTCTC 1 cut(s) 367
BsoBI CYCGRG 2 cut(s) 350, 416
Bsp1286I GDGCHC 3 cut(s) 297, 357, 402
Bsp1407I TGTACA 1 cut(s) 645
Bsp143I GATC 4 cut(s) 9, 160, 664, 715
Bsp19I CCATGG 1 cut(s) 258
BspANI GGCC 3 cut(s) 282, 439, 498
BspCNI CTCAG 2 cut(s) 114, 750
BspDI ATCGAT 1 cut(s) 25
BspFNI CGCG 5 cut(s) 59, 291, 408, 515, 608
BspLI GGNNCC 2 cut(s) 440, 583
BspPI GGATC 2 cut(s) 155, 723
BspTNI GGTCTC 1 cut(s) 367
BsrBI CCGCTC 2 cut(s) 72, 327
BsrFI RCCGGY 2 cut(s) 435, 595
BsrGI TGTACA 1 cut(s) 645
BssAI RCCGGY 2 cut(s) 435, 595
BssECI CCNNGG 4 cut(s) 165, 258, 289, 585
BssMI GATC 4 cut(s) 9, 160, 664, 715
BssT1I CCWWGG 1 cut(s) 258
Bst2UI CCWGG 3 cut(s) 125, 167, 760
Bst4CI ACNGT 3 cut(s) 469, 484, 577
BstAUI TGTACA 1 cut(s) 645
BstBAI YACGTR 2 cut(s) 298, 670
BstC8I GCNNGC 4 cut(s) 293, 458, 532, 597
BstDEI CTNAG 2 cut(s) 101, 737
BstDSI CCRYGG 2 cut(s) 258, 289
BstF5I GGATG 1 cut(s) 309
BstFNI CGCG 5 cut(s) 59, 291, 408, 515, 608
BstH2I RGCGCY 1 cut(s) 684
BstHHI GCGC 3 cut(s) 117, 610, 683
BstKTI GATC 4 cut(s) 12, 163, 667, 718
BstMAI GTCTC 2 cut(s) 223, 367
BstMBI GATC 4 cut(s) 9, 160, 664, 715
BstMCI CGRYCG 3 cut(s) 322, 499, 579
BstMWI GCNNNNNNNGC 7 cut(s) 83, 146, 152, 238, 288, 397, 605
BstNI CCWGG 3 cut(s) 125, 167, 760
BstSCI CCNGG 5 cut(s) 123, 165, 584, 711, 758
BstSLI GKGCMC 1 cut(s) 297
BstUI CGCG 5 cut(s) 59, 291, 408, 515, 608
BstV1I GCAGC 1 cut(s) 250
BstV2I GAAGAC 1 cut(s) 736
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
BstZI CGGCCG 1 cut(s) 496
Bsu15I ATCGAT 1 cut(s) 25
BsuRI GGCC 3 cut(s) 282, 439, 498
BsuTUI ATCGAT 1 cut(s) 25
BtgI CCRYGG 2 cut(s) 258, 289
BtsCI GGATG 1 cut(s) 309
BtsIMutI CAGTG 2 cut(s) 725, 745
Cac8I GCNNGC 4 cut(s) 293, 458, 532, 597
CfoI GCGC 3 cut(s) 117, 610, 683
Cfr10I RCCGGY 2 cut(s) 435, 595
Cfr13I GGNCC 5 cut(s) 169, 433, 438, 480, 756
Cfr42I CCGCGG 1 cut(s) 292
ClaI ATCGAT 1 cut(s) 25
CpoI CGGWCCG 1 cut(s) 433
Csp6I GTAC 1 cut(s) 646
CspI CGGWCCG 1 cut(s) 433
CviAII CATG 2 cut(s) 259, 649
CviQI GTAC 1 cut(s) 646
DdeI CTNAG 2 cut(s) 101, 737
DpnI GATC 4 cut(s) 11, 162, 666, 717
DpnII GATC 4 cut(s) 9, 160, 664, 715
DraIII CACNNNGTG 1 cut(s) 673
DriI GACNNNNNGTC 1 cut(s) 691
EaeI YGGCCR 2 cut(s) 280, 496
EagI CGGCCG 1 cut(s) 496
Eam1105I GACNNNNNGTC 1 cut(s) 691
EciI GGCGGA 2 cut(s) 64, 638
Ecl136II GAGCTC 1 cut(s) 355
EclXI CGGCCG 1 cut(s) 496
Eco130I CCWWGG 1 cut(s) 258
Eco24I GRGCYC 2 cut(s) 357, 402
Eco31I GGTCTC 1 cut(s) 367
Eco47I GGWCC 4 cut(s) 169, 433, 480, 756
Eco47III AGCGCT 1 cut(s) 682
Eco52I CGGCCG 1 cut(s) 496
Eco53kI GAGCTC 1 cut(s) 355
Eco57I CTGAAG 1 cut(s) 575
Eco72I CACGTG 2 cut(s) 298, 670
Eco88I CYCGRG 2 cut(s) 350, 416
EcoICRI GAGCTC 1 cut(s) 355
EcoRII CCWGG 3 cut(s) 123, 165, 758
EcoT14I CCWWGG 1 cut(s) 258
EcoT38I GRGCYC 2 cut(s) 357, 402
ErhI CCWWGG 1 cut(s) 258
Esp3I CGTCTC 1 cut(s) 223
FaeI CATG 2 cut(s) 262, 652
FaiI YATR 2 cut(s) 260, 650
FatI CATG 2 cut(s) 258, 648
FauI CCCGC 3 cut(s) 77, 251, 284
Fnu4HI GCNGC 9 cut(s) 84, 141, 144, 147, 239, 289, 325, 499, 516
FokI GGATG 1 cut(s) 296
FriOI GRGCYC 2 cut(s) 357, 402
Fsp4HI GCNGC 9 cut(s) 84, 141, 144, 147, 239, 289, 325, 499, 516
FspBI CTAG 1 cut(s) 158
FspI TGCGCA 1 cut(s) 116
GlaI GCGC 3 cut(s) 116, 609, 682
GluI GCNGC 9 cut(s) 84, 141, 144, 147, 239, 289, 325, 499, 516
GsaI CCCAGC 3 cut(s) 238, 445, 603
HaeII RGCGCY 1 cut(s) 684
HaeIII GGCC 3 cut(s) 282, 439, 498
HapII CCGG 5 cut(s) 436, 585, 596, 713, 754
HhaI GCGC 3 cut(s) 117, 610, 683
Hin1II CATG 2 cut(s) 262, 652
Hin6I GCGC 3 cut(s) 115, 608, 681
HinP1I GCGC 3 cut(s) 115, 608, 681
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 6 cut(s) 176, 428, 626, 686, 692, 727
HpaII CCGG 5 cut(s) 436, 585, 596, 713, 754
HphI GGTGA 2 cut(s) 553, 652
Hpy166II GTNNAC 2 cut(s) 382, 487
Hpy188I TCNGA 6 cut(s) 433, 581, 619, 691, 705, 726
Hpy188III TCNNGA 5 cut(s) 364, 463, 635, 731, 768
Hpy8I GTNNAC 2 cut(s) 382, 487
HpyAV CCTTC 1 cut(s) 532
HpyCH4III ACNGT 3 cut(s) 469, 484, 577
HpyCH4IV ACGT 2 cut(s) 297, 669
HpyCH4V TGCA 4 cut(s) 309, 391, 423, 565
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 146, 152, 238, 288, 397, 605
HpyF3I CTNAG 2 cut(s) 101, 737
HpySE526I ACGT 2 cut(s) 297, 669
Hsp92II CATG 2 cut(s) 262, 652
HspAI GCGC 3 cut(s) 115, 608, 681
KroI GCCGGC 1 cut(s) 595
KroNI GCCGGC 1 cut(s) 597
KspI CCGCGG 1 cut(s) 292
Kzo9I GATC 4 cut(s) 9, 160, 664, 715
LmnI GCTCC 5 cut(s) 77, 332, 465, 581, 756
Lsp1109I GCAGC 1 cut(s) 250
LweI GCATC 5 cut(s) 318, 378, 540, 552, 640
MaeI CTAG 1 cut(s) 158
MaeII ACGT 2 cut(s) 297, 669
MaeIII GTNAC 1 cut(s) 409
MalI GATC 4 cut(s) 11, 162, 666, 717
MbiI CCGCTC 2 cut(s) 72, 327
MboI GATC 4 cut(s) 9, 160, 664, 715
MboII GAAGA 5 cut(s) 23, 177, 268, 534, 736
MfeI CAATTG 1 cut(s) 392
MflI RGATCY 1 cut(s) 160
MhlI GDGCHC 3 cut(s) 297, 357, 402
MluCI AATT 4 cut(s) 392, 503, 545, 658
MlyI GAGTC 5 cut(s) 437, 635, 680, 701, 736
MmeI TCCRAC 3 cut(s) 411, 559, 597
MroNI GCCGGC 1 cut(s) 595
MseI TTAA 1 cut(s) 708
MslI CAYNNNNRTG 1 cut(s) 418
MspA1I CMGCKG 1 cut(s) 291
MspI CCGG 5 cut(s) 436, 585, 596, 713, 754
MspR9I CCNGG 5 cut(s) 125, 167, 586, 713, 760
MunI CAATTG 1 cut(s) 392
MvaI CCWGG 3 cut(s) 125, 167, 760
MvnI CGCG 5 cut(s) 59, 291, 408, 515, 608
MwoI GCNNNNNNNGC 7 cut(s) 83, 146, 152, 238, 288, 397, 605
NaeI GCCGGC 1 cut(s) 597
NciI CCSGG 2 cut(s) 586, 713
NcoI CCATGG 1 cut(s) 258
NdeII GATC 4 cut(s) 9, 160, 664, 715
NgoMIV GCCGGC 1 cut(s) 595
NlaIII CATG 2 cut(s) 262, 652
NlaIV GGNNCC 2 cut(s) 440, 583
NmuCI GTSAC 1 cut(s) 409
NsbI TGCGCA 1 cut(s) 116
OliI CACNNNNGTG 1 cut(s) 418
PaeR7I CTCGAG 2 cut(s) 350, 416
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 423
PdiI GCCGGC 1 cut(s) 597
PfeI GAWTC 1 cut(s) 176
PkrI GCNGC 9 cut(s) 85, 142, 145, 148, 240, 290, 326, 500, 517
PleI GAGTC 5 cut(s) 436, 634, 680, 700, 735
PmaCI CACGTG 2 cut(s) 298, 670
PmlI CACGTG 2 cut(s) 298, 670
PpsI GAGTC 5 cut(s) 436, 634, 680, 700, 735
Ppu21I YACGTR 2 cut(s) 298, 670
Psp124BI GAGCTC 1 cut(s) 357
Psp6I CCWGG 3 cut(s) 123, 165, 758
PspCI CACGTG 2 cut(s) 298, 670
PspFI CCCAGC 3 cut(s) 234, 441, 599
PspGI CCWGG 3 cut(s) 123, 165, 758
PspN4I GGNNCC 2 cut(s) 440, 583
PspPI GGNCC 5 cut(s) 169, 433, 438, 480, 756
PspXI VCTCGAGB 2 cut(s) 350, 416
PsuI RGATCY 1 cut(s) 160
RsaI GTAC 1 cut(s) 647
RsaNI GTAC 1 cut(s) 646
RseI CAYNNNNRTG 1 cut(s) 418
Rsr2I CGGWCCG 1 cut(s) 433
RsrII CGGWCCG 1 cut(s) 433
SacI GAGCTC 1 cut(s) 357
SacII CCGCGG 1 cut(s) 292
SaqAI TTAA 1 cut(s) 708
SatI GCNGC 9 cut(s) 84, 141, 144, 147, 239, 289, 325, 499, 516
Sau3AI GATC 4 cut(s) 9, 160, 664, 715
Sau96I GGNCC 5 cut(s) 169, 433, 438, 480, 756
SchI GAGTC 5 cut(s) 437, 635, 680, 701, 736
ScrFI CCNGG 5 cut(s) 125, 167, 586, 713, 760
SduI GDGCHC 3 cut(s) 297, 357, 402
SfaNI GCATC 5 cut(s) 318, 378, 540, 552, 640
Sfr274I CTCGAG 2 cut(s) 350, 416
Sfr303I CCGCGG 1 cut(s) 292
SgrBI CCGCGG 1 cut(s) 292
SinI GGWCC 4 cut(s) 169, 433, 480, 756
SlaI CTCGAG 2 cut(s) 350, 416
SmiMI CAYNNNNRTG 1 cut(s) 418
SmlI CTYRAG 2 cut(s) 350, 416
SmoI CTYRAG 2 cut(s) 350, 416
Sse9I AATT 4 cut(s) 392, 503, 545, 658
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 357
StyD4I CCNGG 5 cut(s) 123, 165, 584, 711, 758
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 3 cut(s) 469, 484, 577
TaiI ACGT 2 cut(s) 300, 672
TaqI TCGA 6 cut(s) 25, 251, 351, 365, 417, 569
TaqII GACCGA 1 cut(s) 349
TasI AATT 4 cut(s) 392, 503, 545, 658
TatI WGTACW 1 cut(s) 645
TauI GCSGC 8 cut(s) 86, 143, 146, 149, 291, 327, 501, 518
TfiI GAWTC 1 cut(s) 176
Tru1I TTAA 1 cut(s) 708
Tru9I TTAA 1 cut(s) 708
TscAI CASTG 2 cut(s) 725, 745
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 1 cut(s) 238
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 1 cut(s) 689
TspRI CASTG 2 cut(s) 725, 745
VpaK11BI GGWCC 4 cut(s) 169, 433, 480, 756
XapI RAATTY 1 cut(s) 503
XhoI CTCGAG 2 cut(s) 350, 416
XspI CTAG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.