pycom04g12600

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0016161
Physical Location & Seq
Forward (+)
15511162 .. 15511932
771 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g12600.1

Sequence Viewer

Length: 771 bp
ATGACCAAGATCGACACCCTCCGTCGATTTCTCCTCCCCTGCTTGTCCCCCTCCTCCGCTAAATCCACCCACTCCGCCGCCGCCGCCACCCCCAAGAAACGCCTCAGCACCTCCTTGCGCGACGACCTGGACGACACCAACGCCGCCGCCGCCGCCAAAAACTTAGACCCCAAGGACCAAGATTCACAATCTTCCACCTCGCCCACCCAAAACGACGGCGTGGCGATTCCGCCCCAGCAGACCCGCCCCTCGAAATCCATGGTCATCGGCACAATCTTCGGCCACCGCCGCGGCCACGTGTGGTTGTGCATCCAGCACGACCGCCTCTCCACCAAACCTACCCTCCTCCTCGAGCTCTCGGTCTCGACCCACCAGCTAGTCAACGTGATGCGATTCGGGCTCGTCCGCGTGACCCTCGAGTGCGACGAGTCGGACCGGCCCCAGCTCGCTGATTGCCCGCTCCGATCTGTCCCCATTTGGACCGTTCACTGCAACGGCCGCAAACTCGGGTCCGCGGCAAAAAGAAAAGCGAGCGAGAAGGTGAAATTGATGCTGAAGATGATGCAGTCGACGACGGTCGGAGCCGGGGTAATGCCGGCCGGGTTCGCGCTCGGGTCGGAGGCGGAGTCGGGTCAGGAAGTGATGTACATGAGGGCGAATTATGAGCACGTGGTGGGGAGCGCTGACTCGGAGTCGTTTCATCTGATTAACCCGGATCAGTGCCCGGGTCAAGAACTCAGTGTCTTCCTTCTCCGGTCCAGGCACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

257

Amino Acids

27.96

Weight (kDa)

9.08

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 88 - 252 1e-58 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016161)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37880
fragaria_vesca FvH4_6g12850
malus_domestica MD04G1137600.v1.1 MD12G1152400.v1.1
prunus_persica Prupe.6G263600_v2.0.a1
pyrus_communis pycom04g12600
rosa_chinensis RchiOBHm_Chr3g0464851
rosa_laevigata RLG00000024669
rosa_multiflora Rmu_sc0007114.1_g000004
rosa_roxburghii Rroxscaffold_6G00415390
rosa_rugosa Rorug03G0074400
rosa_samantha Rh3BG137200 Rh3CG139800 Rh3DG138600
rosa_wichuraiana Rw3G011210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 460
AccI GTMKAC 1 cut(s) 569
AccII CGCG 5 cut(s) 120, 291, 408, 515, 608
AclWI GGATC 1 cut(s) 723
AcoI YGGCCR 4 cut(s) 280, 292, 496, 597
AcuI CTGAAG 1 cut(s) 575
AcvI CACGTG 2 cut(s) 298, 670
AdeI CACNNNGTG 1 cut(s) 673
AfaI GTAC 1 cut(s) 647
AfeI AGCGCT 1 cut(s) 682
AflIII ACRYGT 1 cut(s) 297
AhdI GACNNNNNGTC 1 cut(s) 691
AjnI CCWGG 2 cut(s) 126, 758
AluBI AGCT 3 cut(s) 355, 376, 445
AluI AGCT 3 cut(s) 355, 376, 445
Alw21I GWGCWC 2 cut(s) 357, 669
Alw26I GTCTC 1 cut(s) 367
AlwI GGATC 1 cut(s) 723
Ama87I CYCGRG 5 cut(s) 350, 416, 506, 611, 724
Aor51HI AGCGCT 1 cut(s) 682
AoxI GGCC 5 cut(s) 280, 292, 437, 496, 597
AspLEI GCGC 3 cut(s) 120, 610, 683
AspS9I GGNCC 6 cut(s) 175, 433, 438, 480, 510, 756
AsuC2I CCSGG 5 cut(s) 586, 601, 713, 725, 726
AsuHPI GGTGA 1 cut(s) 553
AvaI CYCGRG 5 cut(s) 350, 416, 506, 611, 724
AvaII GGWCC 5 cut(s) 175, 433, 480, 510, 756
BaeGI GKGCMC 1 cut(s) 725
BanII GRGCYC 2 cut(s) 357, 402
BbrPI CACGTG 2 cut(s) 298, 670
BbsI GAAGAC 1 cut(s) 736
Bbv12I GWGCWC 2 cut(s) 357, 669
BbvCI CCTCAGC 1 cut(s) 104
BceAI ACGGC 2 cut(s) 232, 511
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BciT130I CCWGG 2 cut(s) 128, 760
BcnI CCSGG 5 cut(s) 586, 601, 713, 725, 726
BcoDI GTCTC 1 cut(s) 367
BfaI CTAG 1 cut(s) 377
BfoI RGCGCY 1 cut(s) 684
Bme1390I CCNGG 7 cut(s) 128, 586, 601, 713, 725, 726, 760
Bme18I GGWCC 5 cut(s) 175, 433, 480, 510, 756
BmeRI GACNNNNNGTC 1 cut(s) 691
BmeT110I CYCGRG 5 cut(s) 350, 416, 506, 611, 724
BmgT120I GGNCC 6 cut(s) 175, 433, 438, 480, 510, 756
BmiI GGNNCC 3 cut(s) 440, 511, 583
BmrFI CCNGG 7 cut(s) 128, 586, 601, 713, 725, 726, 760
BmsI GCATC 4 cut(s) 318, 378, 540, 552
BoxI GACNNNNGTC 1 cut(s) 575
BpiI GAAGAC 1 cut(s) 736
Bpu10I CCTNAGC 1 cut(s) 104
BpuMI CCSGG 5 cut(s) 586, 601, 713, 725, 726
BsaAI YACGTR 2 cut(s) 298, 670
BsaI GGTCTC 1 cut(s) 367
BsaJI CCNNGG 6 cut(s) 171, 258, 289, 513, 585, 724
BsaWI WCCGGW 1 cut(s) 753
BsaXI ACNNNNNCTCC 8 cut(s) 311, 327, 341, 357, 573, 603, 611, 641
Bse118I RCCGGY 2 cut(s) 435, 595
BseBI CCWGG 2 cut(s) 128, 760
BseDI CCNNGG 6 cut(s) 171, 258, 289, 513, 585, 724
BseGI GGATG 1 cut(s) 309
BseMII CTCAG 2 cut(s) 118, 751
BseRI GAGGAG 4 cut(s) 23, 43, 335, 338
BseSI GKGCMC 1 cut(s) 725
BseX3I CGGCCG 2 cut(s) 496, 597
BseYI CCCAGC 2 cut(s) 234, 441
Bsh1236I CGCG 5 cut(s) 120, 291, 408, 515, 608
Bsh1285I CGRYCG 4 cut(s) 322, 499, 579, 600
BshFI GGCC 5 cut(s) 282, 294, 439, 498, 599
BsiEI CGRYCG 4 cut(s) 322, 499, 579, 600
BsiHKAI GWGCWC 2 cut(s) 357, 669
BsiHKCI CYCGRG 5 cut(s) 350, 416, 506, 611, 724
BsiSI CCGG 7 cut(s) 436, 585, 596, 600, 713, 725, 754
BslFI GGGAC 2 cut(s) 31, 455
BsmAI GTCTC 1 cut(s) 367
BsmFI GGGAC 2 cut(s) 31, 455
BsnI GGCC 5 cut(s) 282, 294, 439, 498, 599
Bso31I GGTCTC 1 cut(s) 367
BsoBI CYCGRG 5 cut(s) 350, 416, 506, 611, 724
Bsp1286I GDGCHC 4 cut(s) 357, 402, 669, 725
Bsp1407I TGTACA 1 cut(s) 645
Bsp143I GATC 3 cut(s) 9, 464, 715
Bsp19I CCATGG 1 cut(s) 258
BspANI GGCC 5 cut(s) 282, 294, 439, 498, 599
BspCNI CTCAG 2 cut(s) 117, 750
BspFNI CGCG 5 cut(s) 120, 291, 408, 515, 608
BspLI GGNNCC 3 cut(s) 440, 511, 583
BspPI GGATC 1 cut(s) 723
BspTNI GGTCTC 1 cut(s) 367
BsrBI CCGCTC 1 cut(s) 460
BsrFI RCCGGY 2 cut(s) 435, 595
BsrGI TGTACA 1 cut(s) 645
BssAI RCCGGY 2 cut(s) 435, 595
BssECI CCNNGG 6 cut(s) 171, 258, 289, 513, 585, 724
BssMI GATC 3 cut(s) 9, 464, 715
BssT1I CCWWGG 2 cut(s) 171, 258
Bst2UI CCWGG 2 cut(s) 128, 760
Bst4CI ACNGT 2 cut(s) 484, 577
BstAUI TGTACA 1 cut(s) 645
BstBAI YACGTR 2 cut(s) 298, 670
BstC8I GCNNGC 4 cut(s) 447, 458, 532, 597
BstDEI CTNAG 3 cut(s) 104, 163, 737
BstDSI CCRYGG 3 cut(s) 258, 289, 513
BstF5I GGATG 1 cut(s) 309
BstFNI CGCG 5 cut(s) 120, 291, 408, 515, 608
BstH2I RGCGCY 1 cut(s) 684
BstHHI GCGC 3 cut(s) 120, 610, 683
BstKTI GATC 3 cut(s) 12, 467, 718
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 3 cut(s) 9, 464, 715
BstMCI CGRYCG 4 cut(s) 322, 499, 579, 600
BstMWI GCNNNNNNNGC 7 cut(s) 83, 149, 152, 288, 397, 498, 605
BstNI CCWGG 2 cut(s) 128, 760
BstPAI GACNNNNGTC 1 cut(s) 575
BstSCI CCNGG 7 cut(s) 126, 584, 599, 711, 723, 724, 758
BstSLI GKGCMC 1 cut(s) 725
BstUI CGCG 5 cut(s) 120, 291, 408, 515, 608
BstV2I GAAGAC 1 cut(s) 736
BstZI CGGCCG 2 cut(s) 496, 597
BsuRI GGCC 5 cut(s) 282, 294, 439, 498, 599
BtgI CCRYGG 3 cut(s) 258, 289, 513
BtsCI GGATG 1 cut(s) 309
BtsI GCAGTG 1 cut(s) 487
BtsIMutI CAGTG 3 cut(s) 487, 725, 745
Cac8I GCNNGC 4 cut(s) 447, 458, 532, 597
CfoI GCGC 3 cut(s) 120, 610, 683
Cfr10I RCCGGY 2 cut(s) 435, 595
Cfr13I GGNCC 6 cut(s) 175, 433, 438, 480, 510, 756
Cfr42I CCGCGG 2 cut(s) 292, 516
Cfr9I CCCGGG 1 cut(s) 724
CpoI CGGWCCG 1 cut(s) 433
Csp6I GTAC 1 cut(s) 646
CspI CGGWCCG 1 cut(s) 433
CviAII CATG 2 cut(s) 259, 649
CviQI GTAC 1 cut(s) 646
DdeI CTNAG 3 cut(s) 104, 163, 737
DpnI GATC 3 cut(s) 11, 466, 717
DpnII GATC 3 cut(s) 9, 464, 715
DraIII CACNNNGTG 1 cut(s) 673
DriI GACNNNNNGTC 1 cut(s) 691
EaeI YGGCCR 4 cut(s) 280, 292, 496, 597
EagI CGGCCG 2 cut(s) 496, 597
Eam1105I GACNNNNNGTC 1 cut(s) 691
EciI GGCGGA 3 cut(s) 64, 219, 638
Ecl136II GAGCTC 1 cut(s) 355
EclXI CGGCCG 2 cut(s) 496, 597
Eco130I CCWWGG 2 cut(s) 171, 258
Eco24I GRGCYC 2 cut(s) 357, 402
Eco31I GGTCTC 1 cut(s) 367
Eco47I GGWCC 5 cut(s) 175, 433, 480, 510, 756
Eco47III AGCGCT 1 cut(s) 682
Eco52I CGGCCG 2 cut(s) 496, 597
Eco53kI GAGCTC 1 cut(s) 355
Eco57I CTGAAG 1 cut(s) 575
Eco72I CACGTG 2 cut(s) 298, 670
Eco88I CYCGRG 5 cut(s) 350, 416, 506, 611, 724
EcoICRI GAGCTC 1 cut(s) 355
EcoRII CCWGG 2 cut(s) 126, 758
EcoT14I CCWWGG 2 cut(s) 171, 258
EcoT38I GRGCYC 2 cut(s) 357, 402
ErhI CCWWGG 2 cut(s) 171, 258
FaeI CATG 2 cut(s) 262, 652
FaiI YATR 3 cut(s) 260, 650, 663
FaqI GGGAC 2 cut(s) 31, 455
FatI CATG 2 cut(s) 258, 648
FauI CCCGC 2 cut(s) 251, 465
FblI GTMKAC 1 cut(s) 569
FokI GGATG 1 cut(s) 296
FriOI GRGCYC 2 cut(s) 357, 402
FspBI CTAG 1 cut(s) 377
GlaI GCGC 3 cut(s) 119, 609, 682
GsaI CCCAGC 2 cut(s) 238, 445
HaeII RGCGCY 1 cut(s) 684
HaeIII GGCC 5 cut(s) 282, 294, 439, 498, 599
HapII CCGG 7 cut(s) 436, 585, 596, 600, 713, 725, 754
HhaI GCGC 3 cut(s) 120, 610, 683
Hin1II CATG 2 cut(s) 262, 652
Hin6I GCGC 3 cut(s) 118, 608, 681
HinP1I GCGC 3 cut(s) 118, 608, 681
HincII GTYRAC 2 cut(s) 382, 570
HindII GTYRAC 2 cut(s) 382, 570
HinfI GANTC 7 cut(s) 182, 226, 393, 428, 626, 686, 692
HpaII CCGG 7 cut(s) 436, 585, 596, 600, 713, 725, 754
HphI GGTGA 1 cut(s) 553
Hpy166II GTNNAC 3 cut(s) 382, 487, 570
Hpy188I TCNGA 6 cut(s) 433, 464, 581, 619, 691, 705
Hpy188III TCNNGA 4 cut(s) 364, 635, 731, 768
Hpy8I GTNNAC 3 cut(s) 382, 487, 570
Hpy99I CGWCG 6 cut(s) 27, 125, 218, 428, 574, 577
HpyAV CCTTC 2 cut(s) 532, 758
HpyCH4III ACNGT 2 cut(s) 484, 577
HpyCH4IV ACGT 3 cut(s) 297, 384, 669
HpyCH4V TGCA 3 cut(s) 309, 492, 565
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 149, 152, 288, 397, 498, 605
HpyF3I CTNAG 3 cut(s) 104, 163, 737
HpySE526I ACGT 3 cut(s) 297, 384, 669
Hsp92II CATG 2 cut(s) 262, 652
HspAI GCGC 3 cut(s) 118, 608, 681
KroI GCCGGC 1 cut(s) 595
KroNI GCCGGC 1 cut(s) 597
KspI CCGCGG 2 cut(s) 292, 516
Kzo9I GATC 3 cut(s) 9, 464, 715
LmnI GCTCC 3 cut(s) 465, 581, 678
LweI GCATC 4 cut(s) 318, 378, 540, 552
MaeI CTAG 1 cut(s) 377
MaeII ACGT 3 cut(s) 297, 384, 669
MaeIII GTNAC 1 cut(s) 409
MalI GATC 3 cut(s) 11, 466, 717
MbiI CCGCTC 1 cut(s) 460
MboI GATC 3 cut(s) 9, 464, 715
MboII GAAGA 4 cut(s) 183, 268, 568, 736
MhlI GDGCHC 4 cut(s) 357, 402, 669, 725
MluCI AATT 2 cut(s) 545, 658
MlyI GAGTC 4 cut(s) 437, 635, 680, 701
MmeI TCCRAC 3 cut(s) 411, 559, 597
MroNI GCCGGC 1 cut(s) 595
MseI TTAA 1 cut(s) 708
MspA1I CMGCKG 2 cut(s) 291, 515
MspI CCGG 7 cut(s) 436, 585, 596, 600, 713, 725, 754
MspR9I CCNGG 7 cut(s) 128, 586, 601, 713, 725, 726, 760
MvaI CCWGG 2 cut(s) 128, 760
MvnI CGCG 5 cut(s) 120, 291, 408, 515, 608
MwoI GCNNNNNNNGC 7 cut(s) 83, 149, 152, 288, 397, 498, 605
NaeI GCCGGC 1 cut(s) 597
NciI CCSGG 5 cut(s) 586, 601, 713, 725, 726
NcoI CCATGG 1 cut(s) 258
NdeII GATC 3 cut(s) 9, 464, 715
NgoMIV GCCGGC 1 cut(s) 595
NlaIII CATG 2 cut(s) 262, 652
NlaIV GGNNCC 3 cut(s) 440, 511, 583
NmuCI GTSAC 1 cut(s) 409
PaeR7I CTCGAG 2 cut(s) 350, 416
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 423
PdiI GCCGGC 1 cut(s) 597
PfeI GAWTC 3 cut(s) 182, 226, 393
PleI GAGTC 4 cut(s) 436, 634, 680, 700
PmaCI CACGTG 2 cut(s) 298, 670
PmlI CACGTG 2 cut(s) 298, 670
PpsI GAGTC 4 cut(s) 436, 634, 680, 700
Ppu21I YACGTR 2 cut(s) 298, 670
PshAI GACNNNNGTC 1 cut(s) 575
Psp124BI GAGCTC 1 cut(s) 357
Psp6I CCWGG 2 cut(s) 126, 758
PspCI CACGTG 2 cut(s) 298, 670
PspFI CCCAGC 2 cut(s) 234, 441
PspGI CCWGG 2 cut(s) 126, 758
PspN4I GGNNCC 3 cut(s) 440, 511, 583
PspPI GGNCC 6 cut(s) 175, 433, 438, 480, 510, 756
PspXI VCTCGAGB 2 cut(s) 350, 416
RsaI GTAC 1 cut(s) 647
RsaNI GTAC 1 cut(s) 646
Rsr2I CGGWCCG 1 cut(s) 433
RsrII CGGWCCG 1 cut(s) 433
SacI GAGCTC 1 cut(s) 357
SacII CCGCGG 2 cut(s) 292, 516
SalI GTCGAC 1 cut(s) 568
SaqAI TTAA 1 cut(s) 708
Sau3AI GATC 3 cut(s) 9, 464, 715
Sau96I GGNCC 6 cut(s) 175, 433, 438, 480, 510, 756
SchI GAGTC 4 cut(s) 437, 635, 680, 701
ScrFI CCNGG 7 cut(s) 128, 586, 601, 713, 725, 726, 760
SduI GDGCHC 4 cut(s) 357, 402, 669, 725
SfaNI GCATC 4 cut(s) 318, 378, 540, 552
Sfr274I CTCGAG 2 cut(s) 350, 416
Sfr303I CCGCGG 2 cut(s) 292, 516
SgrBI CCGCGG 2 cut(s) 292, 516
SinI GGWCC 5 cut(s) 175, 433, 480, 510, 756
SlaI CTCGAG 2 cut(s) 350, 416
SmaI CCCGGG 1 cut(s) 726
SmlI CTYRAG 2 cut(s) 350, 416
SmoI CTYRAG 2 cut(s) 350, 416
Sse9I AATT 2 cut(s) 545, 658
SspMI CTAG 1 cut(s) 377
SstI GAGCTC 1 cut(s) 357
StyD4I CCNGG 7 cut(s) 126, 584, 599, 711, 723, 724, 758
StyI CCWWGG 2 cut(s) 171, 258
TaaI ACNGT 2 cut(s) 484, 577
TaiI ACGT 3 cut(s) 300, 387, 672
TaqI TCGA 7 cut(s) 12, 25, 251, 351, 365, 417, 569
TaqII GACCGA 1 cut(s) 349
TasI AATT 2 cut(s) 545, 658
TatI WGTACW 1 cut(s) 645
TfiI GAWTC 3 cut(s) 182, 226, 393
Tru1I TTAA 1 cut(s) 708
Tru9I TTAA 1 cut(s) 708
TscAI CASTG 3 cut(s) 494, 725, 745
TseFI GTSAC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 1 cut(s) 689
TspGWI ACGGA 1 cut(s) 11
TspMI CCCGGG 1 cut(s) 724
TspRI CASTG 3 cut(s) 494, 725, 745
VpaK11BI GGWCC 5 cut(s) 175, 433, 480, 510, 756
XhoI CTCGAG 2 cut(s) 350, 416
XmaI CCCGGG 1 cut(s) 724
XmiI GTMKAC 1 cut(s) 569
XspI CTAG 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.