Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0010407

Physical Location & Seq

Genomic Coordinates Forward (+)

23506992 .. 23512417

Gene Structure 5426 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1146700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2571 bp

ATGGAATCCGCAGCGATTAGGGCTTTTCTGATCTGTCTAATTCTCTGCGCCGGTTCGAGCTTCGTTCATGGCGAGGAGCCGGTGACGAATAAGTTCCGGGAACGAGAGGCCAGCGACGACGCCCTTGGATATCCGAACATAGATGAGGATGCCTTGTTGAATTCGCGGTGCCCCGCGAAATTGGAGCTCCGATGGCAAACCGAAGTTAGTTCCAGTATATATGCTACACCCTTGATCGCCGATATCAATAGTGATGGAAAGCTTGAGATTGTGGTTCCTTCTTTCGTTCACTATCTTGAAGTTCTTGAAGGTGCTGATGGAGACAAACATCCAGGTTGGCCTGCTTTCCACCAGTCAACGGTACATGCTAGTCCTCTCCTGTATGATATCGACAAGGATGGTGTGAGAGAAATAACTTTGGCCACCTACAATGGTGAAGTTTTATTTTTCAGGGTCTCAGGTTACATGATGGTAGATAAACTTGTAGTACCTCGTAGGAAAGTTAAAAAGAACTGGTATGGGGGTTTGCATTCGGATCCCGTGGATCGTACTCATCCAGATGTCCATGATGATTCTCTTGTCATGGAGGCTATGGAATCAGCTCATCAAACAAATGGAAGTACAGCTAAGTTAAACAATTCTGCTTCTATTTCAACTCCCGATTCCAACACTTCAGCTACTATTTCAACTCCTGATTTAAACAATTCAACAACTATTTCAAAAGAGAGCAACCTTGGCATGGTAAATGCATCAAATCCAGAGAATAAAGGGGAAACAAATTCAAGTCAGGTGGAAACGGTCATCAAGCTGCCTACGGGCACGGACAATTCTTCTGTGAAAAATGTGTCAGAGGAAACTGTTAATGTAGTGAATGGAACTAGTTCTAGTAGACGGCTTTTAGAAGATAAAAATTCAAGTGAATCACAGGATGGTGGTTCTGGATCTAAAGAGAATAATAAGGAGGATGTTCCTGTTGCAACTGTACAAAATGAGGGAAGCTTGGAAGCAGATGCTGATTCATCTTTTGAGTTGTTCCGTAACAGTGAGGAACTGGCCGATGAGTATAGTTATGACTATGATGATTATGTTGATGAATCAATGTGGGGAGATGAGGAATGGGCTGAAGGGCAGCACGAGAAAATGGAGGATTATGTCAATGTTGATGCACACATCCTCTGCACTCCTGTCATAGCGGACATCGATAATGATGGAGTATCAGAAATGGTTGTTGCTGTATCTTACTTCTTTGATCACGAGTATTATGAGTCCAATGAAGATCATCGGAAGGAACTGGGTGACATTGATCTTACAAAATATGTTGCTGGCTCAGTAGTTGTTTTTAATCTCGACACAAAGCAAGTTAAGTGGACTGCGGAACTAGATCTGAGTACAGAGACTGGGAAGTTCCATGCTCATATATATTCTTCTCCAACTGTGGTGGATTTGGATGGTGATGGGAATCTGGACATTCTTGTTGGAACTTCCTTTGGCTTGTTCTATGTCTTGGACCATCATGGCAAGATCAGGGAAAAATTTCCTCTTGAAATGGCTCAAATTCAAGGAGCTGTTGTTGCTGCTGATATCAATGATGATGGAAAAATTGAACTTGTGACCGCTGATGCTCATGGAAATGTTGCTGCATGGACTACAAAAGGAGTAGAAATTTGGGAAAGGCATCTTAAGAGTCTAGTTCCCCAGAGTTTTGTTCCACAGGGTCCTACTATTGGTGATGTTGATGGGGACGGCCATACTGATGTTGTGGTTCCAACGTTATCCGGGAACATATACGTTCTTAGTGGCATAGATGGGTCAATTGTTCGTCCTTATCCATATAGAACTCACGGGAGAGTTATGAATCAAGTTCTTCTAGTTGATTTAAGTAAAAAAGGAGAGAAAAAGAAGGGACTTAGCCTTGTTACAACTTCGTTTGATGGTTACCTGTATGTAATTGATGGACCTACATCATGCGCCGATGTGGTCGATATTGGTGAAACCTCATATAGCATGGTCTTAGCAGACAATGTTGATGGTGGAGATGATCTTGATCTTATTGTTTCAACCATGAATGGCAATGCATGGAGATCACCAAATCAAGGGAGAAATCATGTTGCAAATAGGTATAACCGTGAAGGTATCTTTGTTTCACATTCATCAAGAGCTTTCCGTGACGAAGAGGGCAAGAACTTTTGGGTGGAGATTGAGATCATAGATAGCTATAGATACCCATCTGGGTTGCAAGCACCATATAATGTGACAGCAACGCTATTAGTGCCAGGTAATTACCAAGGAGAGAGAAGGATAAGAATAAACGAGACCTTCAATCGTCCTGGAAAGCATCGTATGAAGCTTCCGACAGTAGGTGTAAGGACTACGGGTACTGTTTTGGTGGAGATGGTTGACAAGAACGGACTCTACTTTTCAGACGATTTCTCACTCACATTCCACATGTATTACTATAGACTCCTCAAGTGGCTGCTTGTCCTACCAATGCTTGGAATGTTCGGCGTGCTTGTAATCCTTCGTCCTCAAGAAGCTGTGCCTTTGCCATCATTTTCGCGAAACACTGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0012505 GO:0016020 GO:0016043 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0043062 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046872 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0071840 GO:0085029

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FG-GAP VCBS

Protein Analysis

857

Amino Acids

94.69

Weight (kDa)

4.7

Isoelectric Point (pI)

33.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FG-GAP_3	PF13517	527 - 588	8e-08	FG-GAP-like repeat
Beta-sand_DEX1	PF23722	711 - 815	2.4e-34	DEX1 beta-sandwich

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010407)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09090 AT3G09090 AT3G09090
fragaria_vesca	FvH4_6g12060
malus_domestica	MD04G1146700.v1.1 MD12G1159900.v1.1
prunus_persica	Prupe.6G270500_v2.0.a1
pyrus_communis	pycom04g13250 pycom12g15180
rosa_chinensis	RchiOBHm_Chr3g0463901 RchiOBHm_Chr3g0463911
rosa_laevigata	RLG00000024737 RLG00000024746
rosa_multiflora	Rmu_sc0000517.1_g000002
rosa_roxburghii	Rroxscaffold_6G00416150
rosa_rugosa	Rorug03G0069100
rosa_samantha	Rh3AG126100 Rh3BG130100 Rh3CG132600 Rh3DG131300
rosa_wichuraiana	Rw3G010640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	168
AccB7I	CCANNNNNTGG	1 cut(s)	2492
AccI	GTMKAC	1 cut(s)	889
AccII	CGCG	3 cut(s)	166, 176, 2557
AciI	CCGC	6 cut(s)	9, 166, 174, 1193, 1373, 1614
AclI	AACGTT	1 cut(s)	1769
AclWI	GGATC	4 cut(s)	530, 543, 552, 949
AcoI	YGGCCR	3 cut(s)	420, 1053, 1744
AcsI	RAATTY	6 cut(s)	160, 778, 910, 1532, 1554, 1662
AcuI	CTGAAG	2 cut(s)	657, 1143
AcyI	GRCGYC	1 cut(s)	120
AfaI	GTAC	7 cut(s)	363, 489, 550, 622, 984, 1390, 2377
AfiI	CCNNNNNNNGG	6 cut(s)	358, 739, 1724, 2093, 2357, 2492
AflII	CTTAAG	1 cut(s)	1679
AflIII	ACRYGT	1 cut(s)	2445
AhlI	ACTAGT	1 cut(s)	878
AjnI	CCWGG	3 cut(s)	331, 2272, 2326
Alw21I	GWGCWC	1 cut(s)	189
Alw26I	GTCTC	4 cut(s)	315, 460, 1388, 2306
AlwI	GGATC	4 cut(s)	530, 543, 552, 949
AlwNI	CAGNNNCTG	2 cut(s)	1013, 1397
AoxI	GGCC	5 cut(s)	108, 338, 420, 1053, 1744
ApeKI	GCWGC	6 cut(s)	11, 808, 1129, 1574, 1637, 2473
ApoI	RAATTY	6 cut(s)	160, 778, 910, 1532, 1554, 1662
Asp700I	GAANNNNTTC	3 cut(s)	92, 880, 1533
AspLEI	GCGC	2 cut(s)	50, 1970
AspS9I	GGNCC	3 cut(s)	1507, 1715, 1955
AsuC2I	CCSGG	2 cut(s)	98, 1777
AsuHPI	GGTGA	7 cut(s)	94, 446, 1307, 1463, 1739, 2000, 2076
AvaII	GGWCC	3 cut(s)	1507, 1715, 1955
BaeGI	GKGCMC	2 cut(s)	173, 821
BalI	TGGCCA	1 cut(s)	422
BamHI	GGATCC	1 cut(s)	535
BanI	GGYRCC	1 cut(s)	168
BanII	GRGCYC	1 cut(s)	189
BauI	CACGAG	2 cut(s)	1133, 1253
Bbv12I	GWGCWC	1 cut(s)	189
BbvI	GCAGC	6 cut(s)	23, 795, 1141, 1561, 1624, 2460
BceAI	ACGGC	2 cut(s)	908, 1759
BciT130I	CCWGG	3 cut(s)	333, 2274, 2328
BclI	TGATCA	1 cut(s)	1249
BcnI	CCSGG	2 cut(s)	98, 1777
BcoDI	GTCTC	4 cut(s)	315, 460, 1388, 2306
BcuI	ACTAGT	1 cut(s)	878
BfaI	CTAG	6 cut(s)	369, 879, 885, 1379, 1688, 1868
BfmI	CTRYAG	2 cut(s)	2215, 2455
BfrI	CTTAAG	1 cut(s)	1679
BglII	AGATCT	1 cut(s)	1381
BisI	GCNGC	6 cut(s)	12, 809, 1130, 1575, 1638, 2474
BlsI	GCNGC	6 cut(s)	13, 810, 1131, 1576, 1639, 2475
Bme1390I	CCNGG	5 cut(s)	98, 333, 1777, 2274, 2328
Bme18I	GGWCC	3 cut(s)	1507, 1715, 1955
BmgT120I	GGNCC	3 cut(s)	1507, 1715, 1955
BmiI	GGNNCC	6 cut(s)	78, 170, 276, 537, 1716, 1764
BmrFI	CCNGG	5 cut(s)	98, 333, 1777, 2274, 2328
BmrI	ACTGGG	2 cut(s)	1301, 1407
BmsI	GCATC	7 cut(s)	139, 758, 1000, 1153, 1609, 1684, 2344
BmuI	ACTGGG	2 cut(s)	1301, 1407
BpuEI	CTTGAG	3 cut(s)	284, 2450, 2511
BpuMI	CCSGG	2 cut(s)	98, 1777
Bsa29I	ATCGAT	1 cut(s)	1200
BsaBI	GATNNNNATC	4 cut(s)	1458, 2043, 2201, 2224
BsaHI	GRCGYC	1 cut(s)	120
BsaI	GGTCTC	2 cut(s)	460, 2306
BsaJI	CCNNGG	4 cut(s)	124, 540, 733, 2284
BsaXI	ACNNNNNCTCC	2 cut(s)	1554, 1584
Bsc4I	CCNNNNNNNGG	6 cut(s)	358, 739, 1724, 2093, 2357, 2492
Bse118I	RCCGGY	2 cut(s)	50, 79
Bse1I	ACTGG	6 cut(s)	213, 352, 518, 1056, 1296, 1402
Bse3DI	GCAATG	1 cut(s)	2077
Bse8I	GATNNNNATC	4 cut(s)	1458, 2043, 2201, 2224
BseBI	CCWGG	3 cut(s)	333, 2274, 2328
BseCI	ATCGAT	1 cut(s)	1200
BseDI	CCNNGG	4 cut(s)	124, 540, 733, 2284
BseGI	GGATG	8 cut(s)	154, 328, 403, 553, 934, 970, 1170, 1453
BseJI	GATNNNNATC	4 cut(s)	1458, 2043, 2201, 2224
BseLI	CCNNNNNNNGG	6 cut(s)	358, 739, 1724, 2093, 2357, 2492
BseMI	GCAATG	1 cut(s)	2077
BseMII	CTCAG	3 cut(s)	471, 1341, 1376
BseNI	ACTGG	6 cut(s)	213, 352, 518, 1056, 1296, 1402
BseRI	GAGGAG	2 cut(s)	89, 2453
BseSI	GKGCMC	2 cut(s)	173, 821
BseXI	GCAGC	6 cut(s)	23, 795, 1141, 1561, 1624, 2460
BsgI	GTGCAG	1 cut(s)	1162
Bsh1236I	CGCG	3 cut(s)	166, 176, 2557
BshFI	GGCC	5 cut(s)	110, 340, 422, 1055, 1746
BshNI	GGYRCC	1 cut(s)	168
BshVI	ATCGAT	1 cut(s)	1200
BsiHKAI	GWGCWC	1 cut(s)	189
BsiSI	CCGG	4 cut(s)	51, 80, 97, 1776
BslFI	GGGAC	2 cut(s)	1754, 1917
BslI	CCNNNNNNNGG	6 cut(s)	358, 739, 1724, 2093, 2357, 2492
BsmAI	GTCTC	4 cut(s)	315, 460, 1388, 2306
BsmFI	GGGAC	2 cut(s)	1754, 1917
BsmI	GAATGC	1 cut(s)	529
BsnI	GGCC	5 cut(s)	110, 340, 422, 1055, 1746
Bso31I	GGTCTC	2 cut(s)	460, 2306
Bsp1286I	GDGCHC	3 cut(s)	173, 189, 821
Bsp1407I	TGTACA	1 cut(s)	982
Bsp68I	TCGCGA	1 cut(s)	2557
BspACI	CCGC	6 cut(s)	9, 166, 174, 1193, 1373, 1614
BspANI	GGCC	5 cut(s)	110, 340, 422, 1055, 1746
BspCNI	CTCAG	3 cut(s)	470, 1340, 1377
BspDI	ATCGAT	1 cut(s)	1200
BspFNI	CGCG	3 cut(s)	166, 176, 2557
BspLI	GGNNCC	6 cut(s)	78, 170, 276, 537, 1716, 1764
BspPI	GGATC	4 cut(s)	530, 543, 552, 949
BspT107I	GGYRCC	1 cut(s)	168
BspTI	CTTAAG	1 cut(s)	1679
BspTNI	GGTCTC	2 cut(s)	460, 2306
BsrDI	GCAATG	1 cut(s)	2077
BsrFI	RCCGGY	2 cut(s)	50, 79
BsrGI	TGTACA	1 cut(s)	982
BsrI	ACTGG	6 cut(s)	213, 352, 518, 1056, 1296, 1402
BssAI	RCCGGY	2 cut(s)	50, 79
BssECI	CCNNGG	4 cut(s)	124, 540, 733, 2284
BssNI	GRCGYC	1 cut(s)	120
BssSI	CACGAG	2 cut(s)	1133, 1253
BssT1I	CCWWGG	3 cut(s)	124, 733, 2284
Bst2BI	CACGAG	2 cut(s)	1133, 1253
Bst2UI	CCWGG	3 cut(s)	333, 2274, 2328
Bst4CI	ACNGT	9 cut(s)	361, 799, 859, 982, 1043, 1435, 2126, 2356, 2380
Bst6I	CTCTTC	1 cut(s)	2166
BstACI	GRCGYC	1 cut(s)	120
BstAFI	CTTAAG	1 cut(s)	1679
BstAUI	TGTACA	1 cut(s)	982
BstC8I	GCNNGC	5 cut(s)	112, 342, 1324, 2238, 2507
BstDEI	CTNAG	7 cut(s)	457, 627, 1327, 1385, 1793, 1907, 2011
BstDSI	CCRYGG	1 cut(s)	540
BstEII	GGTNACC	1 cut(s)	1934
BstF5I	GGATG	8 cut(s)	154, 328, 403, 553, 934, 970, 1170, 1453
BstFNI	CGCG	3 cut(s)	166, 176, 2557
BstHHI	GCGC	2 cut(s)	50, 1970
BstMAI	GTCTC	4 cut(s)	315, 460, 1388, 2306
BstMWI	GCNNNNNNNGC	5 cut(s)	20, 193, 735, 1571, 2269
BstNI	CCWGG	3 cut(s)	333, 2274, 2328
BstNSI	RCATGY	2 cut(s)	368, 2449
BstPI	GGTNACC	1 cut(s)	1934
BstSCI	CCNGG	5 cut(s)	96, 331, 1775, 2272, 2326
BstSFI	CTRYAG	2 cut(s)	2215, 2455
BstSLI	GKGCMC	2 cut(s)	173, 821
BstUI	CGCG	3 cut(s)	166, 176, 2557
BstV1I	GCAGC	6 cut(s)	23, 795, 1141, 1561, 1624, 2460
BstX2I	RGATCY	3 cut(s)	535, 941, 1381
BstYI	RGATCY	3 cut(s)	535, 941, 1381
Bsu15I	ATCGAT	1 cut(s)	1200
BsuRI	GGCC	5 cut(s)	110, 340, 422, 1055, 1746
BsuTUI	ATCGAT	1 cut(s)	1200
BtgI	CCRYGG	1 cut(s)	540
BtsCI	GGATG	8 cut(s)	154, 328, 403, 553, 934, 970, 1170, 1453
BtsIMutI	CAGTG	2 cut(s)	1048, 2562
BtuMI	TCGCGA	1 cut(s)	2557
Cac8I	GCNNGC	5 cut(s)	112, 342, 1324, 2238, 2507
CaiI	CAGNNNCTG	2 cut(s)	1013, 1397
CfoI	GCGC	2 cut(s)	50, 1970
Cfr10I	RCCGGY	2 cut(s)	50, 79
Cfr13I	GGNCC	3 cut(s)	1507, 1715, 1955
ClaI	ATCGAT	1 cut(s)	1200
CseI	GACGC	1 cut(s)	128
Csp6I	GTAC	7 cut(s)	362, 488, 549, 621, 983, 1389, 2376
CspCI	CAANNNNNGTGG	6 cut(s)	771, 806, 1346, 1381, 1419, 1454
CviQI	GTAC	7 cut(s)	362, 488, 549, 621, 983, 1389, 2376
DdeI	CTNAG	7 cut(s)	457, 627, 1327, 1385, 1793, 1907, 2011
DraI	TTTAAA	1 cut(s)	699
EaeI	YGGCCR	3 cut(s)	420, 1053, 1744
Eam1104I	CTCTTC	1 cut(s)	2166
EarI	CTCTTC	1 cut(s)	2166
Ecl136II	GAGCTC	1 cut(s)	187
Eco130I	CCWWGG	3 cut(s)	124, 733, 2284
Eco24I	GRGCYC	1 cut(s)	189
Eco31I	GGTCTC	2 cut(s)	460, 2306
Eco32I	GATATC	4 cut(s)	131, 244, 388, 1582
Eco47I	GGWCC	3 cut(s)	1507, 1715, 1955
Eco53kI	GAGCTC	1 cut(s)	187
Eco57I	CTGAAG	2 cut(s)	657, 1143
Eco91I	GGTNACC	1 cut(s)	1934
EcoICRI	GAGCTC	1 cut(s)	187
EcoO109I	RGGNCCY	1 cut(s)	1715
EcoO65I	GGTNACC	1 cut(s)	1934
EcoRI	GAATTC	1 cut(s)	160
EcoRII	CCWGG	3 cut(s)	331, 2272, 2326
EcoRV	GATATC	4 cut(s)	131, 244, 388, 1582
EcoT14I	CCWWGG	3 cut(s)	124, 733, 2284
EcoT22I	ATGCAT	2 cut(s)	751, 2077
EcoT38I	GRGCYC	1 cut(s)	189
ErhI	CCWWGG	3 cut(s)	124, 733, 2284
FaqI	GGGAC	2 cut(s)	1754, 1917
FauI	CCCGC	1 cut(s)	181
FbaI	TGATCA	1 cut(s)	1249
FblI	GTMKAC	1 cut(s)	889
Fnu4HI	GCNGC	6 cut(s)	12, 809, 1130, 1575, 1638, 2474
FokI	GGATG	8 cut(s)	161, 315, 410, 540, 941, 977, 1157, 1460
FriOI	GRGCYC	1 cut(s)	189
Fsp4HI	GCNGC	6 cut(s)	12, 809, 1130, 1575, 1638, 2474
FspBI	CTAG	6 cut(s)	369, 879, 885, 1379, 1688, 1868
GlaI	GCGC	2 cut(s)	49, 1969
GluI	GCNGC	6 cut(s)	12, 809, 1130, 1575, 1638, 2474
HaeIII	GGCC	5 cut(s)	110, 340, 422, 1055, 1746
HapII	CCGG	4 cut(s)	51, 80, 97, 1776
HgaI	GACGC	1 cut(s)	128
HhaI	GCGC	2 cut(s)	50, 1970
Hin1I	GRCGYC	1 cut(s)	120
Hin6I	GCGC	2 cut(s)	48, 1968
HinP1I	GCGC	2 cut(s)	48, 1968
HincII	GTYRAC	2 cut(s)	357, 2398
HindII	GTYRAC	2 cut(s)	357, 2398
HindIII	AAGCTT	3 cut(s)	260, 997, 2345
HpaII	CCGG	4 cut(s)	51, 80, 97, 1776
HphI	GGTGA	7 cut(s)	94, 446, 1307, 1463, 1739, 2000, 2076
Hpy166II	GTNNAC	5 cut(s)	289, 357, 890, 1368, 2398
Hpy8I	GTNNAC	5 cut(s)	289, 357, 890, 1368, 2398
Hpy99I	CGWCG	2 cut(s)	119, 122
HpyAV	CCTTC	9 cut(s)	288, 302, 1118, 1279, 1894, 2123, 2289, 2326, 2528
HpyCH4III	ACNGT	9 cut(s)	361, 799, 859, 982, 1043, 1435, 2126, 2356, 2380
HpyCH4IV	ACGT	2 cut(s)	1769, 1788
HpyCH4V	TGCA	9 cut(s)	529, 749, 977, 1166, 1179, 1640, 2075, 2111, 2236
HpyF10VI	GCNNNNNNNGC	5 cut(s)	20, 193, 735, 1571, 2269
HpyF3I	CTNAG	7 cut(s)	457, 627, 1327, 1385, 1793, 1907, 2011
HpySE526I	ACGT	2 cut(s)	1769, 1788
Hsp92I	GRCGYC	1 cut(s)	120
HspAI	GCGC	2 cut(s)	48, 1968
Ksp22I	TGATCA	1 cut(s)	1249
LmnI	GCTCC	4 cut(s)	76, 184, 192, 1562
Lsp1109I	GCAGC	6 cut(s)	23, 795, 1141, 1561, 1624, 2460
LweI	GCATC	7 cut(s)	139, 758, 1000, 1153, 1609, 1684, 2344
MaeI	CTAG	6 cut(s)	369, 879, 885, 1379, 1688, 1868
MaeII	ACGT	2 cut(s)	1769, 1788
MaeIII	GTNAC	9 cut(s)	82, 461, 1037, 1295, 1609, 1915, 1934, 2165, 2251
MboII	GAAGA	6 cut(s)	822, 914, 1286, 1416, 1856, 2183
MfeI	CAATTG	1 cut(s)	1812
MflI	RGATCY	3 cut(s)	535, 941, 1381
MhlI	GDGCHC	3 cut(s)	173, 189, 821
MlsI	TGGCCA	1 cut(s)	422
MluNI	TGGCCA	1 cut(s)	422
MlyI	GAGTC	4 cut(s)	1274, 1693, 2403, 2454
MmeI	TCCRAC	5 cut(s)	690, 1454, 1456, 1790, 2375
Mox20I	TGGCCA	1 cut(s)	422
Mph1103I	ATGCAT	2 cut(s)	751, 2077
MroXI	GAANNNNTTC	3 cut(s)	92, 880, 1533
MscI	TGGCCA	1 cut(s)	422
MseI	TTAA	8 cut(s)	504, 632, 698, 861, 1341, 1362, 1680, 1877
MslI	CAYNNNNRTG	3 cut(s)	558, 1629, 1752
Msp20I	TGGCCA	1 cut(s)	422
MspA1I	CMGCKG	1 cut(s)	1616
MspCI	CTTAAG	1 cut(s)	1679
MspI	CCGG	4 cut(s)	51, 80, 97, 1776
MspR9I	CCNGG	5 cut(s)	98, 333, 1777, 2274, 2328
MunI	CAATTG	1 cut(s)	1812
Mva1269I	GAATGC	1 cut(s)	529
MvaI	CCWGG	3 cut(s)	333, 2274, 2328
MvnI	CGCG	3 cut(s)	166, 176, 2557
MwoI	GCNNNNNNNGC	5 cut(s)	20, 193, 735, 1571, 2269
NciI	CCSGG	2 cut(s)	98, 1777
NlaIV	GGNNCC	6 cut(s)	78, 170, 276, 537, 1716, 1764
NmuCI	GTSAC	5 cut(s)	82, 1295, 1609, 2165, 2251
NruI	TCGCGA	1 cut(s)	2557
NsiI	ATGCAT	2 cut(s)	751, 2077
NspI	RCATGY	2 cut(s)	368, 2449
PciI	ACATGT	1 cut(s)	2445
PcsI	WCGNNNNNNNCGW	1 cut(s)	69
PctI	GAATGC	1 cut(s)	529
PdmI	GAANNNNTTC	3 cut(s)	92, 880, 1533
PfeI	GAWTC	9 cut(s)	5, 572, 596, 662, 920, 1016, 1094, 1459, 1855
PflMI	CCANNNNNTGG	1 cut(s)	2492
PfoI	TCCNGGA	3 cut(s)	96, 1775, 2326
PkrI	GCNGC	6 cut(s)	13, 810, 1131, 1576, 1639, 2475
PleI	GAGTC	4 cut(s)	1273, 1692, 2403, 2454
PpsI	GAGTC	4 cut(s)	1273, 1692, 2403, 2454
PpuMI	RGGWCCY	1 cut(s)	1715
PscI	ACATGT	1 cut(s)	2445
Psp124BI	GAGCTC	1 cut(s)	189
Psp1406I	AACGTT	1 cut(s)	1769
Psp5II	RGGWCCY	1 cut(s)	1715
Psp6I	CCWGG	3 cut(s)	331, 2272, 2326
PspEI	GGTNACC	1 cut(s)	1934
PspGI	CCWGG	3 cut(s)	331, 2272, 2326
PspN4I	GGNNCC	6 cut(s)	78, 170, 276, 537, 1716, 1764
PspPI	GGNCC	3 cut(s)	1507, 1715, 1955
PspPPI	RGGWCCY	1 cut(s)	1715
PsrI	GAACNNNNNNTAC	2 cut(s)	2396, 2428
PstNI	CAGNNNCTG	2 cut(s)	1013, 1397
PsuI	RGATCY	3 cut(s)	535, 941, 1381
RruI	TCGCGA	1 cut(s)	2557
RsaI	GTAC	7 cut(s)	363, 489, 550, 622, 984, 1390, 2377
RsaNI	GTAC	7 cut(s)	362, 488, 549, 621, 983, 1389, 2376
RseI	CAYNNNNRTG	3 cut(s)	558, 1629, 1752
SacI	GAGCTC	1 cut(s)	189
SaqAI	TTAA	8 cut(s)	504, 632, 698, 861, 1341, 1362, 1680, 1877
SatI	GCNGC	6 cut(s)	12, 809, 1130, 1575, 1638, 2474
Sau96I	GGNCC	3 cut(s)	1507, 1715, 1955
SchI	GAGTC	4 cut(s)	1274, 1693, 2403, 2454
ScrFI	CCNGG	5 cut(s)	98, 333, 1777, 2274, 2328
SduI	GDGCHC	3 cut(s)	173, 189, 821
SfaNI	GCATC	7 cut(s)	139, 758, 1000, 1153, 1609, 1684, 2344
SfcI	CTRYAG	2 cut(s)	2215, 2455
SinI	GGWCC	3 cut(s)	1507, 1715, 1955
SmiMI	CAYNNNNRTG	3 cut(s)	558, 1629, 1752
SmlI	CTYRAG	4 cut(s)	263, 1679, 2465, 2526
SmoI	CTYRAG	4 cut(s)	263, 1679, 2465, 2526
SpeI	ACTAGT	1 cut(s)	878
SsiI	CCGC	6 cut(s)	9, 166, 174, 1193, 1373, 1614
SspMI	CTAG	6 cut(s)	369, 879, 885, 1379, 1688, 1868
SstI	GAGCTC	1 cut(s)	189
StyD4I	CCNGG	5 cut(s)	96, 331, 1775, 2272, 2326
StyI	CCWWGG	3 cut(s)	124, 733, 2284
TaaI	ACNGT	9 cut(s)	361, 799, 859, 982, 1043, 1435, 2126, 2356, 2380
TaiI	ACGT	2 cut(s)	1772, 1791
TaqI	TCGA	5 cut(s)	56, 390, 1200, 1347, 1980
TatI	WGTACW	3 cut(s)	620, 982, 1388
TfiI	GAWTC	9 cut(s)	5, 572, 596, 662, 920, 1016, 1094, 1459, 1855
Tru1I	TTAA	8 cut(s)	504, 632, 698, 861, 1341, 1362, 1680, 1877
Tru9I	TTAA	8 cut(s)	504, 632, 698, 861, 1341, 1362, 1680, 1877
TscAI	CASTG	2 cut(s)	1048, 2569
TseFI	GTSAC	5 cut(s)	82, 1295, 1609, 2165, 2251
TseI	GCWGC	6 cut(s)	11, 808, 1129, 1574, 1637, 2473
Tsp45I	GTSAC	5 cut(s)	82, 1295, 1609, 2165, 2251
TspDTI	ATGAA	8 cut(s)	56, 1008, 1107, 1287, 1868, 2078, 2139, 2357
TspGWI	ACGGA	4 cut(s)	836, 1025, 2153, 2421
TspRI	CASTG	2 cut(s)	1048, 2569
Van91I	CCANNNNNTGG	1 cut(s)	2492
Vha464I	CTTAAG	1 cut(s)	1679
VpaK11BI	GGWCC	3 cut(s)	1507, 1715, 1955
XapI	RAATTY	6 cut(s)	160, 778, 910, 1532, 1554, 1662
XceI	RCATGY	2 cut(s)	368, 2449
XmiI	GTMKAC	1 cut(s)	889
XmnI	GAANNNNTTC	3 cut(s)	92, 880, 1533
XspI	CTAG	6 cut(s)	369, 879, 885, 1379, 1688, 1868
Zsp2I	ATGCAT	2 cut(s)	751, 2077

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.