Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0010407

Physical Location & Seq

Genomic Coordinates Forward (+)

38204168 .. 38210934

Gene Structure 6767 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00416150.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2628 bp

ATGAAATCCGACGCGATTCGGGTGATTTTTCTGATCTGTTTAATTCTTTGCGACGGCTCGAAATTCGTTCGCGGCGCCGATGAGCCGGCCAAGAATAAATTCCGGGAACGCGAGGCCAGCGACGACTCCCTTGGCTATCCAAACATAGATGAGGATGCGTTGTTGAATACACAGTGCCCTGCGAAATTGGAGCTCAGATGGCAAACGGAAGTTAGTTCTAGTGTTTATGCTACGCCATTGATCTCCGATATTAATAGTGATGGAAAGCTTGAGATTGTGGTGCCATCTTTTGTTCACTATCTTGAAGTTTTGGAAGGTTCCGACGGAGACAAACTCCCCGGTTGGCCTGCTTTCCATCAGTCAACTGTACATGCAAGTCCTCTCCTGTATGATATCGATAAGGATGGTGTGAGAGAGATAGCTTTAGCAACCTACAATGGTGAAGTTCTCTTTTTCAGGGTCTCAGGCTACATGATGGTAGATAAACTAGTAGTACCTCGTAGGAAAGTTAAAAAGAATTGGTTTGGGGGTTTGCATCCAGATCCCGTTGATCGTACCCATCCAGATGTTCAGGATGACCTTCTTGTCATGCAAGCCACTAATATGAATTCAAATCCTCAAACAGATGAAAATACAACTAAAATAAACAATTCTGCTACTGTTGCAACAGAAAGCCATCCTGGTGTTCATCCTGGTGTTAATACTTCAACTTCTGTTTCCAATGATGGCCATCCTGAGTTAGATACTAATACTACTGTTTCAAAAGAAAGTGATCCCGGCTTAAGTACTCCAGCTAAAAACGAAAGCCACCTTAGCATGGTCAAGGCATCAAATCCAGAGGTTGAGAAGAAAGCAAATTTAAGTCCGTTAGAAACAGACATCAAGCAATCTACAAGCACAGAGAAGTCTTCTGTCAACAATGTGCCAGTGGAAACTCTGAATGCCAAGAAGGGGACTAGTTCGGTTAGACGGCTTTTGGAGGACAACAATTTAAGCAAATCACAGGATGGTGGTTCTGAATCCAAAGACAATAATGAGGACATTCCTGTTGCAACTGTAGAAAATGATGGTGTTCTCGAAGAGGATGCTGATTCATCTTTTGAGTTACTCCGTCACAATGATGAACTGGCCGACGAGTATAACTATGACTATGATGATTATGTTGATGAAGAAATGTGGGGAGATGAGGAGTGGACTGAAGTGCAACACGAGAAAATAGAAGATTATGTCAATGTTGACGCACACATCTTGTGCACTCCTGTCATAGCGGACATTGATAATGATGGAATATCAGAAATGGTTGTTGCCGTTTCTTACTTTTTTGATCATGAGTACTATGACAACCCAGAGCATATGAAGGAATTGGGTGGCATTGATATAGGAAAATATGTAGCTGGTTCCATTGTTGTGTTCAACCTTGACACTAAGCAAGTAAAGTGGACTGCTGATCTAGATTTGAGTACAGAGACTGGAACATTCCGTGCTTACATATATTCCTCTCCGACTGTCGTTGATTTGGATGGTGATGGGAACCTGGACATTCTTGTTGGAACTTCATTTGGCTTGTTCTATGTGTTAGATCATCATGGCAAGGTGAGGGAAAAGTTCCCTCTTGAAATGGCTGAAATTCAAGGAGCTGTTGTTGCAGCTGATATTAATGATGATGGAAAAATTGAACTAGTGACTACTGATGCTCATGGAAATGTTGCTGCATGGACTGCTCAAGGAGTAGAAATTTGGGAAACGCATCTTAAGAGTCTAGTTCCCCAGGGTCCCACCATTGGTGATGTTGACGGGGATGGCCGTACTGATGTTGTGGTTCCAACACTATCAGGGAACATATACGTTCTTAGTGGCAAAGATGGGTCGATTGTTCGTCCTTATCCATATAGAACTCATGGGAGGGTTATGAGTCAGGTTCTTCTAGTGGATTTAAGTAAGAAAGGAGAGAAAAAGAAAGGACTCACTCTTGCTACAACATCATTTGATGGATATTTGTACCTTATTGATGGACCAACAGCATGTGCAGATGTTGTTGATATTGGTGAAACTTCATATAGCATGGTCTTAGCAGATAATGTTGACGGTGGAGACGATCTTGATCTTATTGTTACAACCATGAATGGCAATGCATGGAGAGTACCTAATCAGGGGAGAAATCATCTTGCAAATAGGTACAACCGTCAAGGTATCTTTGTTAAACATTCATCAAGAGCTTTTCGTGACGAAGAGGGCAAGAACTTCTTTGTGGAGATTGAGATCATAGATGACTATAGATACCCATCTGGGTTGCAAGCTCCATACACTGTCACAACAACCTTGTTGGTGCCTGGTAATTACCAAGGAGAGAGAAGGATAAAGATAAACCAGACCTTCAATCGTCCTGGAAAGTATCGTATAAAGCTTCCAACCGTTGGTGTAAGGACTACTGGGACTGTTGTGGTCGAGATGGTTGACAAGAATGGACTCTATTTTTCAGATGATTTCTCCCTCACATTCCATATGTATTACTACAGACTGTTAAAGTGGCTGCTTGTCCTGCCGATGTTGGGGATGTTTGGCGTGCTTGTAATCCTTCGTCCTCAGGAAGCTGTGCCTTTGCCATCATTTTCACGAAACACTGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0012505 GO:0016020 GO:0016043 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0043062 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046872 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0071840 GO:0085029

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FG-GAP VCBS

Protein Analysis

875

Amino Acids

97.09

Weight (kDa)

4.68

Isoelectric Point (pI)

32.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FG-GAP_3	PF13517	551 - 607	1.3e-06	FG-GAP-like repeat
Beta-sand_DEX1	PF23722	730 - 834	3.4e-34	DEX1 beta-sandwich

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010407)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09090 AT3G09090 AT3G09090
fragaria_vesca	FvH4_6g12060
malus_domestica	MD04G1146700.v1.1 MD12G1159900.v1.1
prunus_persica	Prupe.6G270500_v2.0.a1
pyrus_communis	pycom04g13250 pycom12g15180
rosa_chinensis	RchiOBHm_Chr3g0463901 RchiOBHm_Chr3g0463911
rosa_laevigata	RLG00000024737 RLG00000024746
rosa_multiflora	Rmu_sc0000517.1_g000002
rosa_roxburghii	Rroxscaffold_6G00416150
rosa_rugosa	Rorug03G0069100
rosa_samantha	Rh3AG126100 Rh3BG130100 Rh3CG132600 Rh3DG131300
rosa_wichuraiana	Rw3G010640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	584
AccB1I	GGYRCC	3 cut(s)	74, 280, 2326
AccB7I	CCANNNNNTGG	1 cut(s)	2414
AccII	CGCG	3 cut(s)	14, 72, 111
AciI	CCGC	2 cut(s)	72, 1268
AclWI	GGATC	2 cut(s)	536, 767
AcoI	YGGCCR	4 cut(s)	87, 727, 1128, 1801
AcsI	RAATTY	6 cut(s)	62, 98, 607, 856, 1626, 1734
AcuI	CTGAAG	1 cut(s)	1218
AcyI	GRCGYC	1 cut(s)	75
AfiI	CCNNNNNNNGG	6 cut(s)	817, 951, 1781, 2150, 2414, 2549
AflII	CTTAAG	2 cut(s)	781, 1751
AhlI	ACTAGT	3 cut(s)	487, 956, 1678
AjnI	CCWGG	6 cut(s)	679, 691, 1533, 1767, 2329, 2383
AjuI	GAANNNNNNNTTGG	2 cut(s)	83, 115
Alw21I	GWGCWC	2 cut(s)	195, 1256
Alw26I	GTCTC	4 cut(s)	321, 466, 1460, 2085
Alw44I	GTGCAC	1 cut(s)	1252
AlwI	GGATC	2 cut(s)	536, 767
AlwNI	CAGNNNCTG	1 cut(s)	1469
AoxI	GGCC	6 cut(s)	87, 114, 344, 727, 1128, 1801
ApaLI	GTGCAC	1 cut(s)	1252
ApeKI	GCWGC	3 cut(s)	1646, 1709, 2530
ApoI	RAATTY	6 cut(s)	62, 98, 607, 856, 1626, 1734
AseI	ATTAAT	2 cut(s)	252, 1656
Asp700I	GAANNNNTTC	1 cut(s)	98
AspLEI	GCGC	1 cut(s)	77
AspS9I	GGNCC	2 cut(s)	1772, 2012
AsuC2I	CCSGG	3 cut(s)	104, 339, 777
AsuHPI	GGTGA	6 cut(s)	34, 452, 1535, 1606, 1796, 2057
AvaII	GGWCC	2 cut(s)	1772, 2012
AxyI	CCTNAGG	1 cut(s)	2583
BaeGI	GKGCMC	2 cut(s)	179, 1256
BaeI	ACNNNNGTAYC	2 cut(s)	2167, 2200
BalI	TGGCCA	1 cut(s)	729
BanI	GGYRCC	3 cut(s)	74, 280, 2326
BanII	GRGCYC	1 cut(s)	195
BauI	CACGAG	1 cut(s)	1208
BbsI	GAAGAC	1 cut(s)	900
Bbv12I	GWGCWC	2 cut(s)	195, 1256
BbvI	GCAGC	3 cut(s)	1658, 1696, 2517
BceAI	ACGGC	4 cut(s)	70, 986, 1292, 1788
BcgI	CGANNNNNNTGC	2 cut(s)	1067, 1101
BciT130I	CCWGG	6 cut(s)	681, 693, 1535, 1769, 2331, 2385
BclI	TGATCA	1 cut(s)	1324
BcnI	CCSGG	3 cut(s)	104, 339, 777
BcoDI	GTCTC	4 cut(s)	321, 466, 1460, 2085
BcuI	ACTAGT	3 cut(s)	487, 956, 1678
BfaI	CTAG	7 cut(s)	219, 488, 957, 1451, 1679, 1760, 1925
BfmI	CTRYAG	3 cut(s)	1056, 2272, 2512
BfoI	RGCGCY	1 cut(s)	78
BfrI	CTTAAG	2 cut(s)	781, 1751
BisI	GCNGC	4 cut(s)	73, 1647, 1710, 2531
BlsI	GCNGC	4 cut(s)	74, 1648, 1711, 2532
BmcAI	AGTACT	2 cut(s)	787, 1334
Bme1390I	CCNGG	9 cut(s)	104, 339, 681, 693, 777, 1535, 1769, 2331, 2385
Bme18I	GGWCC	2 cut(s)	1772, 2012
BmgT120I	GGNCC	2 cut(s)	1772, 2012
BmiI	GGNNCC	9 cut(s)	76, 282, 319, 1399, 1532, 1773, 1774, 1821, 2328
BmrFI	CCNGG	9 cut(s)	104, 339, 681, 693, 777, 1535, 1769, 2331, 2385
BmrI	ACTGGG	1 cut(s)	2439
BmsI	GCATC	6 cut(s)	145, 544, 836, 1075, 1681, 1756
BmuI	ACTGGG	1 cut(s)	2439
BpiI	GAAGAC	1 cut(s)	900
BplI	GAGNNNNNCTC	2 cut(s)	318, 350
BpmI	CTGGAG	1 cut(s)	774
Bpu10I	CCTNAGC	1 cut(s)	812
BpuEI	CTTGAG	2 cut(s)	290, 1707
BpuMI	CCSGG	3 cut(s)	104, 339, 777
Bsa29I	ATCGAT	1 cut(s)	396
BsaBI	GATNNNNATC	4 cut(s)	729, 2100, 2258, 2281
BsaHI	GRCGYC	1 cut(s)	75
BsaI	GGTCTC	1 cut(s)	466
BsaJI	CCNNGG	5 cut(s)	130, 337, 1767, 1768, 2341
BsaXI	ACNNNNNCTCC	2 cut(s)	1626, 1656
Bsc4I	CCNNNNNNNGG	6 cut(s)	817, 951, 1781, 2150, 2414, 2549
Bse118I	RCCGGY	1 cut(s)	85
Bse1I	ACTGG	4 cut(s)	926, 1131, 1474, 2434
Bse21I	CCTNAGG	1 cut(s)	2583
Bse3DI	GCAATG	1 cut(s)	2134
Bse8I	GATNNNNATC	4 cut(s)	729, 2100, 2258, 2281
BseBI	CCWGG	6 cut(s)	681, 693, 1535, 1769, 2331, 2385
BseCI	ATCGAT	1 cut(s)	396
BseDI	CCNNGG	5 cut(s)	130, 337, 1767, 1768, 2341
BseJI	GATNNNNATC	4 cut(s)	729, 2100, 2258, 2281
BseLI	CCNNNNNNNGG	6 cut(s)	817, 951, 1781, 2150, 2414, 2549
BseMI	GCAATG	1 cut(s)	2134
BseMII	CTCAG	4 cut(s)	208, 477, 726, 2597
BseNI	ACTGG	4 cut(s)	926, 1131, 1474, 2434
BseRI	GAGGAG	1 cut(s)	1202
BseSI	GKGCMC	2 cut(s)	179, 1256
BseXI	GCAGC	3 cut(s)	1658, 1696, 2517
BsgI	GTGCAG	1 cut(s)	2046
Bsh1236I	CGCG	3 cut(s)	14, 72, 111
BshFI	GGCC	6 cut(s)	89, 116, 346, 729, 1130, 1803
BshNI	GGYRCC	3 cut(s)	74, 280, 2326
BshVI	ATCGAT	1 cut(s)	396
BsiHKAI	GWGCWC	2 cut(s)	195, 1256
BsiSI	CCGG	4 cut(s)	86, 103, 339, 777
BslFI	GGGAC	3 cut(s)	967, 1758, 2446
BslI	CCNNNNNNNGG	6 cut(s)	817, 951, 1781, 2150, 2414, 2549
BsmAI	GTCTC	4 cut(s)	321, 466, 1460, 2085
BsmBI	CGTCTC	1 cut(s)	2085
BsmFI	GGGAC	3 cut(s)	967, 1758, 2446
BsmI	GAATGC	1 cut(s)	946
BsnI	GGCC	6 cut(s)	89, 116, 346, 729, 1130, 1803
Bso31I	GGTCTC	1 cut(s)	466
Bsp1286I	GDGCHC	3 cut(s)	179, 195, 1256
Bsp1407I	TGTACA	1 cut(s)	367
BspACI	CCGC	2 cut(s)	72, 1268
BspANI	GGCC	6 cut(s)	89, 116, 346, 729, 1130, 1803
BspCNI	CTCAG	4 cut(s)	207, 476, 727, 2596
BspDI	ATCGAT	1 cut(s)	396
BspFNI	CGCG	3 cut(s)	14, 72, 111
BspHI	TCATGA	1 cut(s)	1327
BspLI	GGNNCC	9 cut(s)	76, 282, 319, 1399, 1532, 1773, 1774, 1821, 2328
BspPI	GGATC	2 cut(s)	536, 767
BspT107I	GGYRCC	3 cut(s)	74, 280, 2326
BspTI	CTTAAG	2 cut(s)	781, 1751
BspTNI	GGTCTC	1 cut(s)	466
BsrDI	GCAATG	1 cut(s)	2134
BsrFI	RCCGGY	1 cut(s)	85
BsrGI	TGTACA	1 cut(s)	367
BsrI	ACTGG	4 cut(s)	926, 1131, 1474, 2434
BssAI	RCCGGY	1 cut(s)	85
BssECI	CCNNGG	5 cut(s)	130, 337, 1767, 1768, 2341
BssNI	GRCGYC	1 cut(s)	75
BssSI	CACGAG	1 cut(s)	1208
BssT1I	CCWWGG	2 cut(s)	130, 2341
Bst2BI	CACGAG	1 cut(s)	1208
Bst2UI	CCWGG	6 cut(s)	681, 693, 1535, 1769, 2331, 2385
Bst6I	CTCTTC	2 cut(s)	1074, 2223
BstACI	GRCGYC	1 cut(s)	75
BstAFI	CTTAAG	2 cut(s)	781, 1751
BstAPI	GCANNNNNTGC	1 cut(s)	1718
BstAUI	TGTACA	1 cut(s)	367
BstC8I	GCNNGC	6 cut(s)	87, 118, 348, 594, 2295, 2564
BstDEI	CTNAG	8 cut(s)	194, 463, 735, 812, 1425, 1850, 2068, 2583
BstFNI	CGCG	3 cut(s)	14, 72, 111
BstH2I	RGCGCY	1 cut(s)	78
BstHHI	GCGC	1 cut(s)	77
BstMAI	GTCTC	4 cut(s)	321, 466, 1460, 2085
BstMWI	GCNNNNNNNGC	7 cut(s)	117, 199, 662, 813, 1643, 1718, 2539
BstNI	CCWGG	6 cut(s)	681, 693, 1535, 1769, 2331, 2385
BstNSI	RCATGY	2 cut(s)	374, 2025
BstSCI	CCNGG	9 cut(s)	102, 337, 679, 691, 775, 1533, 1767, 2329, 2383
BstSFI	CTRYAG	3 cut(s)	1056, 2272, 2512
BstSLI	GKGCMC	2 cut(s)	179, 1256
BstUI	CGCG	3 cut(s)	14, 72, 111
BstV1I	GCAGC	3 cut(s)	1658, 1696, 2517
BstV2I	GAAGAC	1 cut(s)	900
BstX2I	RGATCY	1 cut(s)	541
BstYI	RGATCY	1 cut(s)	541
Bsu15I	ATCGAT	1 cut(s)	396
Bsu36I	CCTNAGG	1 cut(s)	2583
BsuRI	GGCC	6 cut(s)	89, 116, 346, 729, 1130, 1803
BsuTUI	ATCGAT	1 cut(s)	396
BtsIMutI	CAGTG	4 cut(s)	179, 933, 2304, 2619
Cac8I	GCNNGC	6 cut(s)	87, 118, 348, 594, 2295, 2564
CaiI	CAGNNNCTG	1 cut(s)	1469
CciI	TCATGA	1 cut(s)	1327
CfoI	GCGC	1 cut(s)	77
Cfr10I	RCCGGY	1 cut(s)	85
Cfr13I	GGNCC	2 cut(s)	1772, 2012
ClaI	ATCGAT	1 cut(s)	396
CseI	GACGC	2 cut(s)	20, 1247
CspCI	CAANNNNNGTGG	2 cut(s)	1418, 1453
DdeI	CTNAG	8 cut(s)	194, 463, 735, 812, 1425, 1850, 2068, 2583
DinI	GGCGCC	1 cut(s)	76
DrdI	GACNNNNNNGTC	1 cut(s)	584
DseDI	GACNNNNNNGTC	1 cut(s)	584
EaeI	YGGCCR	4 cut(s)	87, 727, 1128, 1801
Eam1104I	CTCTTC	2 cut(s)	1074, 2223
EarI	CTCTTC	2 cut(s)	1074, 2223
Ecl136II	GAGCTC	1 cut(s)	193
Eco130I	CCWWGG	2 cut(s)	130, 2341
Eco24I	GRGCYC	1 cut(s)	195
Eco31I	GGTCTC	1 cut(s)	466
Eco32I	GATATC	1 cut(s)	394
Eco47I	GGWCC	2 cut(s)	1772, 2012
Eco53kI	GAGCTC	1 cut(s)	193
Eco57I	CTGAAG	1 cut(s)	1218
Eco81I	CCTNAGG	1 cut(s)	2583
EcoICRI	GAGCTC	1 cut(s)	193
EcoO109I	RGGNCCY	1 cut(s)	1772
EcoRI	GAATTC	1 cut(s)	607
EcoRII	CCWGG	6 cut(s)	679, 691, 1533, 1767, 2329, 2383
EcoRV	GATATC	1 cut(s)	394
EcoT14I	CCWWGG	2 cut(s)	130, 2341
EcoT22I	ATGCAT	1 cut(s)	2134
EcoT38I	GRGCYC	1 cut(s)	195
EgeI	GGCGCC	1 cut(s)	76
EheI	GGCGCC	1 cut(s)	76
ErhI	CCWWGG	2 cut(s)	130, 2341
Esp3I	CGTCTC	1 cut(s)	2085
FalI	AAGNNNNNCTT	2 cut(s)	2228, 2260
FaqI	GGGAC	3 cut(s)	967, 1758, 2446
FauNDI	CATATG	2 cut(s)	1353, 2502
FbaI	TGATCA	1 cut(s)	1324
Fnu4HI	GCNGC	4 cut(s)	73, 1647, 1710, 2531
FriOI	GRGCYC	1 cut(s)	195
Fsp4HI	GCNGC	4 cut(s)	73, 1647, 1710, 2531
FspBI	CTAG	7 cut(s)	219, 488, 957, 1451, 1679, 1760, 1925
GlaI	GCGC	1 cut(s)	76
GluI	GCNGC	4 cut(s)	73, 1647, 1710, 2531
GsuI	CTGGAG	1 cut(s)	774
HaeII	RGCGCY	1 cut(s)	78
HaeIII	GGCC	6 cut(s)	89, 116, 346, 729, 1130, 1803
HapII	CCGG	4 cut(s)	86, 103, 339, 777
HgaI	GACGC	2 cut(s)	20, 1247
HhaI	GCGC	1 cut(s)	77
Hin1I	GRCGYC	1 cut(s)	75
Hin6I	GCGC	1 cut(s)	75
HinP1I	GCGC	1 cut(s)	75
HincII	GTYRAC	6 cut(s)	363, 916, 1237, 1792, 2083, 2455
HindII	GTYRAC	6 cut(s)	363, 916, 1237, 1792, 2083, 2455
HindIII	AAGCTT	2 cut(s)	266, 2402
HinfI	GANTC	8 cut(s)	16, 125, 1019, 1091, 1756, 1912, 1962, 2466
HpaII	CCGG	4 cut(s)	86, 103, 339, 777
HphI	GGTGA	6 cut(s)	34, 452, 1535, 1606, 1796, 2057
Hpy99I	CGWCG	5 cut(s)	14, 56, 125, 326, 1136
HpyAV	CCTTC	7 cut(s)	308, 590, 943, 1351, 2346, 2383, 2585
HpyCH4IV	ACGT	1 cut(s)	1845
HpyF10VI	GCNNNNNNNGC	7 cut(s)	117, 199, 662, 813, 1643, 1718, 2539
HpyF3I	CTNAG	8 cut(s)	194, 463, 735, 812, 1425, 1850, 2068, 2583
HpySE526I	ACGT	1 cut(s)	1845
Hsp92I	GRCGYC	1 cut(s)	75
HspAI	GCGC	1 cut(s)	75
KasI	GGCGCC	1 cut(s)	74
KflI	GGGWCCC	1 cut(s)	1772
KroI	GCCGGC	1 cut(s)	85
KroNI	GCCGGC	1 cut(s)	87
Ksp22I	TGATCA	1 cut(s)	1324
LmnI	GCTCC	3 cut(s)	190, 1634, 2302
Lsp1109I	GCAGC	3 cut(s)	1658, 1696, 2517
LweI	GCATC	6 cut(s)	145, 544, 836, 1075, 1681, 1756
MaeI	CTAG	7 cut(s)	219, 488, 957, 1451, 1679, 1760, 1925
MaeII	ACGT	1 cut(s)	1845
MaeIII	GTNAC	6 cut(s)	1104, 1112, 1681, 2110, 2222, 2308
MboII	GAAGA	7 cut(s)	859, 900, 1091, 1181, 1232, 1913, 2240
MflI	RGATCY	1 cut(s)	541
MhlI	GDGCHC	3 cut(s)	179, 195, 1256
MlsI	TGGCCA	1 cut(s)	729
MluNI	TGGCCA	1 cut(s)	729
Mly113I	GGCGCC	1 cut(s)	75
MlyI	GAGTC	5 cut(s)	119, 1765, 1921, 1956, 2460
MmeI	TCCRAC	6 cut(s)	33, 345, 1526, 1528, 1847, 2432
Mox20I	TGGCCA	1 cut(s)	729
Mph1103I	ATGCAT	1 cut(s)	2134
MroNI	GCCGGC	1 cut(s)	85
MroXI	GAANNNNTTC	1 cut(s)	98
MscI	TGGCCA	1 cut(s)	729
MslI	CAYNNNNRTG	7 cut(s)	564, 602, 681, 693, 1119, 1406, 1701
Msp20I	TGGCCA	1 cut(s)	729
MspA1I	CMGCKG	1 cut(s)	1649
MspCI	CTTAAG	2 cut(s)	781, 1751
MspI	CCGG	4 cut(s)	86, 103, 339, 777
MspR9I	CCNGG	9 cut(s)	104, 339, 681, 693, 777, 1535, 1769, 2331, 2385
Mva1269I	GAATGC	1 cut(s)	946
MvaI	CCWGG	6 cut(s)	681, 693, 1535, 1769, 2331, 2385
MvnI	CGCG	3 cut(s)	14, 72, 111
MwoI	GCNNNNNNNGC	7 cut(s)	117, 199, 662, 813, 1643, 1718, 2539
NaeI	GCCGGC	1 cut(s)	87
NarI	GGCGCC	1 cut(s)	75
NciI	CCSGG	3 cut(s)	104, 339, 777
NdeI	CATATG	2 cut(s)	1353, 2502
NgoMIV	GCCGGC	1 cut(s)	85
NlaIV	GGNNCC	9 cut(s)	76, 282, 319, 1399, 1532, 1773, 1774, 1821, 2328
NmuCI	GTSAC	4 cut(s)	1112, 1681, 2222, 2308
NsiI	ATGCAT	1 cut(s)	2134
NspI	RCATGY	2 cut(s)	374, 2025
PagI	TCATGA	1 cut(s)	1327
PasI	CCCWGGG	1 cut(s)	1768
PcsI	WCGNNNNNNNCGW	1 cut(s)	2091
PctI	GAATGC	1 cut(s)	946
PdiI	GCCGGC	1 cut(s)	87
PdmI	GAANNNNTTC	1 cut(s)	98
PfeI	GAWTC	3 cut(s)	16, 1019, 1091
PflMI	CCANNNNNTGG	1 cut(s)	2414
PfoI	TCCNGGA	2 cut(s)	102, 2383
PkrI	GCNGC	4 cut(s)	74, 1648, 1711, 2532
PleI	GAGTC	5 cut(s)	119, 1764, 1920, 1956, 2460
PluTI	GGCGCC	1 cut(s)	78
PpsI	GAGTC	5 cut(s)	119, 1764, 1920, 1956, 2460
PpuMI	RGGWCCY	1 cut(s)	1772
PshBI	ATTAAT	2 cut(s)	252, 1656
Psp124BI	GAGCTC	1 cut(s)	195
Psp5II	RGGWCCY	1 cut(s)	1772
Psp6I	CCWGG	6 cut(s)	679, 691, 1533, 1767, 2329, 2383
PspGI	CCWGG	6 cut(s)	679, 691, 1533, 1767, 2329, 2383
PspN4I	GGNNCC	9 cut(s)	76, 282, 319, 1399, 1532, 1773, 1774, 1821, 2328
PspPI	GGNCC	2 cut(s)	1772, 2012
PspPPI	RGGWCCY	1 cut(s)	1772
PstNI	CAGNNNCTG	1 cut(s)	1469
PsuI	RGATCY	1 cut(s)	541
PvuII	CAGCTG	1 cut(s)	1649
RseI	CAYNNNNRTG	7 cut(s)	564, 602, 681, 693, 1119, 1406, 1701
SacI	GAGCTC	1 cut(s)	195
SatI	GCNGC	4 cut(s)	73, 1647, 1710, 2531
Sau96I	GGNCC	2 cut(s)	1772, 2012
ScaI	AGTACT	2 cut(s)	787, 1334
SchI	GAGTC	5 cut(s)	119, 1765, 1921, 1956, 2460
ScrFI	CCNGG	9 cut(s)	104, 339, 681, 693, 777, 1535, 1769, 2331, 2385
SduI	GDGCHC	3 cut(s)	179, 195, 1256
SfaNI	GCATC	6 cut(s)	145, 544, 836, 1075, 1681, 1756
SfcI	CTRYAG	3 cut(s)	1056, 2272, 2512
SfoI	GGCGCC	1 cut(s)	76
SinI	GGWCC	2 cut(s)	1772, 2012
SmiMI	CAYNNNNRTG	7 cut(s)	564, 602, 681, 693, 1119, 1406, 1701
SmlI	CTYRAG	4 cut(s)	269, 781, 1722, 1751
SmoI	CTYRAG	4 cut(s)	269, 781, 1722, 1751
SpeI	ACTAGT	3 cut(s)	487, 956, 1678
SsiI	CCGC	2 cut(s)	72, 1268
SspDI	GGCGCC	1 cut(s)	74
SspMI	CTAG	7 cut(s)	219, 488, 957, 1451, 1679, 1760, 1925
SstI	GAGCTC	1 cut(s)	195
StyD4I	CCNGG	9 cut(s)	102, 337, 679, 691, 775, 1533, 1767, 2329, 2383
StyI	CCWWGG	2 cut(s)	130, 2341
TaiI	ACGT	1 cut(s)	1848
TaqI	TCGA	5 cut(s)	59, 396, 1077, 1868, 2445
TatI	WGTACW	4 cut(s)	367, 785, 1332, 1460
TauI	GCSGC	1 cut(s)	75
TfiI	GAWTC	3 cut(s)	16, 1019, 1091
TscAI	CASTG	4 cut(s)	179, 933, 2311, 2626
TseFI	GTSAC	4 cut(s)	1112, 1681, 2222, 2308
TseI	GCWGC	3 cut(s)	1646, 1709, 2530
Tsp45I	GTSAC	4 cut(s)	1112, 1681, 2222, 2308
TspGWI	ACGGA	5 cut(s)	221, 339, 855, 1100, 1469
TspRI	CASTG	4 cut(s)	179, 933, 2311, 2626
Van91I	CCANNNNNTGG	1 cut(s)	2414
Vha464I	CTTAAG	2 cut(s)	781, 1751
VneI	GTGCAC	1 cut(s)	1252
VpaK11BI	GGWCC	2 cut(s)	1772, 2012
VspI	ATTAAT	2 cut(s)	252, 1656
XapI	RAATTY	6 cut(s)	62, 98, 607, 856, 1626, 1734
XbaI	TCTAGA	1 cut(s)	1450
XceI	RCATGY	2 cut(s)	374, 2025
XmnI	GAANNNNTTC	1 cut(s)	98
XspI	CTAG	7 cut(s)	219, 488, 957, 1451, 1679, 1760, 1925
ZrmI	AGTACT	2 cut(s)	787, 1334
Zsp2I	ATGCAT	1 cut(s)	2134

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.