Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0010407

Physical Location & Seq

Genomic Coordinates Forward (+)

23977652 .. 23983157

Gene Structure 5506 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1159900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2640 bp

ATGAAATTCCCGGCGATTAGGGCTTTTCTGATCTGTCTAATTCTCTGCGCCGGTTCGAGCTTCGTTCACGGCGAGGAGCCGGCGAAGAATAAGTTCCGGGAACGAGAAGCCAGCGACGACTCCCTTGGATATCCGAACATAGATGAGGATGCATTGTTGAATACGCAGTGCCCTGCGAAATTGGAGCTCAGATGGCAAACGGAAGTTAGTTCTAGTATATATGCTACGCCCTTGATCGCCGATATCAATAGTGATGGAAAGCTTGAGATTGTGGTTTCTTCTTTCGTTCACTATCTTGAAGTTCTTGAAGGTGCTGATGGAGACAAACATCCCGGTTGGCCTGCTTTCCATCAGTCAACCGTACATGCGAGTCCTCTCCTGCATGATATCGACAAGGACGGTGTGAGAGAAATAACTTTGGCAACCTACAATGGTGAAGTTTTATTTTTCAGGGTTTCAGGTTACATGATGGTAGATAAACTTGTAGTACCTCGTAGGAAAGTTAAAAAGAACTGGTATGGGGGTCTGCATCCGGATCCCGTGGATCGCACTCATCCAGATGTCCATGATGATTCTCTTGTCATGGAGGCTTTGGAATCAATTCATCAAACAAATGGAAATACAGCTAAATTAAACAATTCTGCTTCTATTTCAACTTCTGATTCAAACACTTCAACTATTATTTCAACTCCTGATACAAACAATTCAGCTACTATTTCAAAAGCGAGCAACCTTGGCATGGTAAATGCATCAAATCCAGAGAATAAAGGGGAAACAAATTCAACTCAGGTGGAAACAGTCATCAAGCTGCCTACAGGCACGGACAATTCTTCTGTGAAAAATGTGTCAGAGGAAACTGTTAATGTAGTGAATGGAACTAGTTCTGGGAGACGGCTTTTAGAAGATAAAAAGTCAAGTGAATCGCAGGAGGGTAGTTCTGAACCCAGAGATAATAATAAGGAGGATGTTCCTGTTGCAACTGTACAAAATGAGGGAAGCTTGGAAGCAGATGCTGATTCATCTTTTGAGTTGTTCCGTAGCAGTGAGGAACTGGCTGATGAATATAGTTATGACTATGATGACTATGCTGATGAATCAATGTGGGGAGATGAGGAATGGGCTGAAGAGCAGCACGAGAAAATGGAGGATTATGTCGATGTTGATGCACACATCCTCTGCACTCCTGTCATAGCGGACATAGACAATGATGGAGTATCAGAAATGGTTGTTGCTGTATCTTACTTCTTTGATAGCGAGTATTATGAGTCCAACGAAGGTCATCGAAAGGAATTGGGTGACATTGATATTACAAAATATGTTGCTGGCTCAATAGTTGTGTTTAATCTCGACACAAAGCAAGTTAAGTGGACTGCGGAACTAGATCTGAGTACAGAGACTGCGACGTTCCGTGCTCATATATATTCTTCTCCAACTGTAGTGGATTTGGATGGTGACGGGAATCTGGACATTCTTGTCGGAACTTCCTTTGGCTTGTTCTATTTCTTGGATCATCATGGCAAGGTCAGAGAAAAATTTCCTCTTGAAATGGCTGAAATTCAAGGAGCTGTTGTTGCTGCTGATATCAATGATGATGGAAAAATTGAACTAGTGACTACTGATGCTCATGGAAATGTTGCTGCATGGACTACAAAAGGAGTAGAAATTTGGGAAAATCATCTTAAGAGTCTAGTTCCCCAGGGTCCCACTATTGGTGATGTTGATGGGGATGGCCATACTGATGTTGTGGTTCCAACTTTATCCGGCAACATATATGTTCTTAGTGGCAAAGATGGGTCAATTGTTCGTCCTTATCCATATAGAACTCATGGGAGAGTTATGAATCAAGTTCTTCTAGTTGATTTAAGTAAAAAGGGAGAGAAAAAGAAGGGACTCACCCTTGTTACAACTTCGTTTGATGGTTACTTGTACCTAATTGATGGACCTACATCATGCGCCGATGTGGTCGACATTGGTGAAACTTCATATAGCATGGTCTTAGCAGACAATGTTGATGGTGGAGATGATCTTGATCTTATTGTTTCAACCATGAATGGCAATGCATGGAGATCACCTAATCAAGGAAGAAATCATGTTGCAAATAGGCATAACCGTGAAGGTATCTTTGTTTCACATTCATCAAGAGCTTTCCGTGATGAAGAAGGCAAGAACTTTTGGGTGGAGTTTGAGATCATAGATAGCTATAGATACCCATCTGGGTTGCAAGCACCCTATAATGTCACAGTAAGTTGCTCATTCCCACCAATTATTTTGGCCAAAGTCGACTACACAGTCCTCCCTCCCTCTGTCTCTTGTATACACACGCAGGTAACGCTGTTAGTGCCAGGTAATTACCAAGGAGAGAGAAGGATAAGAATAAACGAGACCTACAATCGTCCTGGAAAGTATCGTATGAAGCTTCCGACAGTTGGTGTAAGGACTATGGGTACTGTTTTGGTGGAGATGGTTGACAAGAACGGACTCTATTTTTCAGATGATTTCTCCCTCACATTCCACATGTATTACTATAGACTGTTGAAATGGCTGCTCGTCCTACCGATGCTTGGAATGTTTGGCATACTTGTAATCCTACGTCCTCAAGAAGCTGTGCCTTTGCCATCATTTTCGCGAAACGCTGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0012505 GO:0016020 GO:0016043 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0043062 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046872 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0071840 GO:0085029

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FG-GAP VCBS

Protein Analysis

880

Amino Acids

97.4

Weight (kDa)

4.72

Isoelectric Point (pI)

33.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FG-GAP_3	PF13517	527 - 583	2.4e-06	FG-GAP-like repeat
Beta-sand_DEX1	PF23722	706 - 838	1.3e-33	DEX1 beta-sandwich

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010407)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09090 AT3G09090 AT3G09090
fragaria_vesca	FvH4_6g12060
malus_domestica	MD04G1146700.v1.1 MD12G1159900.v1.1
prunus_persica	Prupe.6G270500_v2.0.a1
pyrus_communis	pycom04g13250 pycom12g15180
rosa_chinensis	RchiOBHm_Chr3g0463901 RchiOBHm_Chr3g0463911
rosa_laevigata	RLG00000024737 RLG00000024746
rosa_multiflora	Rmu_sc0000517.1_g000002
rosa_roxburghii	Rroxscaffold_6G00416150
rosa_rugosa	Rorug03G0069100
rosa_samantha	Rh3AG126100 Rh3BG130100 Rh3CG132600 Rh3DG131300
rosa_wichuraiana	Rw3G010640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1472, 2288
Acc36I	ACCTGC	1 cut(s)	2314
AccI	GTMKAC	3 cut(s)	1965, 2280, 2314
AccII	CGCG	1 cut(s)	2626
AccIII	TCCGGA	1 cut(s)	532
AciI	CCGC	2 cut(s)	1193, 1373
AclWI	GGATC	4 cut(s)	530, 543, 552, 1515
AcoI	YGGCCR	2 cut(s)	1729, 2271
AcsI	RAATTY	5 cut(s)	5, 778, 1532, 1554, 1662
AcuI	CTGAAG	1 cut(s)	1143
AfaI	GTAC	6 cut(s)	363, 489, 984, 1390, 1928, 2446
AfiI	CCNNNNNNNGG	5 cut(s)	739, 1709, 2078, 2426, 2561
AflII	CTTAAG	1 cut(s)	1679
AflIII	ACRYGT	1 cut(s)	2514
AhlI	ACTAGT	2 cut(s)	878, 1606
AjnI	CCWGG	3 cut(s)	1695, 2341, 2395
Alw21I	GWGCWC	2 cut(s)	189, 1414
Alw26I	GTCTC	5 cut(s)	315, 883, 1388, 2311, 2375
AlwI	GGATC	4 cut(s)	530, 543, 552, 1515
AlwNI	CAGNNNCTG	2 cut(s)	1013, 1397
Aor13HI	TCCGGA	1 cut(s)	532
AoxI	GGCC	3 cut(s)	338, 1729, 2271
ApeKI	GCWGC	5 cut(s)	808, 1129, 1574, 1637, 2542
ApoI	RAATTY	5 cut(s)	5, 778, 1532, 1554, 1662
Asp700I	GAANNNNTTC	4 cut(s)	92, 600, 880, 1533
AspLEI	GCGC	2 cut(s)	50, 1955
AspS9I	GGNCC	2 cut(s)	1700, 1940
AsuC2I	CCSGG	3 cut(s)	11, 98, 333
AsuHPI	GGTGA	7 cut(s)	446, 1307, 1463, 1724, 1885, 1985, 2061
AvaII	GGWCC	2 cut(s)	1700, 1940
BaeGI	GKGCMC	1 cut(s)	173
BalI	TGGCCA	2 cut(s)	1731, 2273
BamHI	GGATCC	1 cut(s)	535
BanII	GRGCYC	1 cut(s)	189
BauI	CACGAG	1 cut(s)	1133
Bbv12I	GWGCWC	2 cut(s)	189, 1414
BbvI	GCAGC	5 cut(s)	795, 1141, 1561, 1624, 2529
BceAI	ACGGC	2 cut(s)	85, 908
BcgI	CGANNNNNNTGC	2 cut(s)	1145, 1179
BciT130I	CCWGG	3 cut(s)	1697, 2343, 2397
BcnI	CCSGG	3 cut(s)	11, 98, 333
BcoDI	GTCTC	5 cut(s)	315, 883, 1388, 2311, 2375
BcuI	ACTAGT	2 cut(s)	878, 1606
BfaI	CTAG	6 cut(s)	213, 879, 1379, 1607, 1688, 1853
BfmI	CTRYAG	4 cut(s)	813, 1434, 2200, 2524
BfrI	CTTAAG	1 cut(s)	1679
BfuAI	ACCTGC	1 cut(s)	2314
BglII	AGATCT	1 cut(s)	1381
BisI	GCNGC	5 cut(s)	809, 1130, 1575, 1638, 2543
BlsI	GCNGC	5 cut(s)	810, 1131, 1576, 1639, 2544
Bme1390I	CCNGG	6 cut(s)	11, 98, 333, 1697, 2343, 2397
Bme18I	GGWCC	2 cut(s)	1700, 1940
BmgT120I	GGNCC	2 cut(s)	1700, 1940
BmiI	GGNNCC	5 cut(s)	78, 537, 1701, 1702, 1749
BmrFI	CCNGG	6 cut(s)	11, 98, 333, 1697, 2343, 2397
BmsI	GCATC	7 cut(s)	139, 538, 758, 1000, 1153, 1609, 2547
BpuEI	CTTGAG	2 cut(s)	284, 2580
BpuMI	CCSGG	3 cut(s)	11, 98, 333
BsaBI	GATNNNNATC	2 cut(s)	2028, 2209
BsaI	GGTCTC	1 cut(s)	2375
BsaJI	CCNNGG	6 cut(s)	124, 540, 733, 1695, 1696, 2353
BsaWI	WCCGGW	1 cut(s)	532
BsaXI	ACNNNNNCTCC	2 cut(s)	1554, 1584
Bsc4I	CCNNNNNNNGG	5 cut(s)	739, 1709, 2078, 2426, 2561
Bse118I	RCCGGY	2 cut(s)	50, 79
Bse1I	ACTGG	2 cut(s)	518, 1056
Bse3DI	GCAATG	1 cut(s)	2062
Bse8I	GATNNNNATC	2 cut(s)	2028, 2209
BseAI	TCCGGA	1 cut(s)	532
BseBI	CCWGG	3 cut(s)	1697, 2343, 2397
BseDI	CCNNGG	6 cut(s)	124, 540, 733, 1695, 1696, 2353
BseGI	GGATG	8 cut(s)	154, 328, 529, 553, 970, 1170, 1453, 1732
BseJI	GATNNNNATC	2 cut(s)	2028, 2209
BseLI	CCNNNNNNNGG	5 cut(s)	739, 1709, 2078, 2426, 2561
BseMI	GCAATG	1 cut(s)	2062
BseMII	CTCAG	3 cut(s)	202, 800, 1376
BseNI	ACTGG	2 cut(s)	518, 1056
BseRI	GAGGAG	1 cut(s)	89
BseSI	GKGCMC	1 cut(s)	173
BseXI	GCAGC	5 cut(s)	795, 1141, 1561, 1624, 2529
BsgI	GTGCAG	1 cut(s)	1162
Bsh1236I	CGCG	1 cut(s)	2626
BshFI	GGCC	3 cut(s)	340, 1731, 2273
BsiHKAI	GWGCWC	2 cut(s)	189, 1414
BsiSI	CCGG	7 cut(s)	11, 51, 80, 97, 333, 533, 1761
BslFI	GGGAC	2 cut(s)	1686, 1902
BslI	CCNNNNNNNGG	5 cut(s)	739, 1709, 2078, 2426, 2561
BsmAI	GTCTC	5 cut(s)	315, 883, 1388, 2311, 2375
BsmBI	CGTCTC	1 cut(s)	883
BsmFI	GGGAC	2 cut(s)	1686, 1902
BsnI	GGCC	3 cut(s)	340, 1731, 2273
Bso31I	GGTCTC	1 cut(s)	2375
Bsp1286I	GDGCHC	3 cut(s)	173, 189, 1414
Bsp13I	TCCGGA	1 cut(s)	532
Bsp1407I	TGTACA	1 cut(s)	982
Bsp68I	TCGCGA	1 cut(s)	2626
BspACI	CCGC	2 cut(s)	1193, 1373
BspANI	GGCC	3 cut(s)	340, 1731, 2273
BspCNI	CTCAG	3 cut(s)	201, 799, 1377
BspEI	TCCGGA	1 cut(s)	532
BspFNI	CGCG	1 cut(s)	2626
BspLI	GGNNCC	5 cut(s)	78, 537, 1701, 1702, 1749
BspMI	ACCTGC	1 cut(s)	2314
BspPI	GGATC	4 cut(s)	530, 543, 552, 1515
BspQI	GCTCTTC	1 cut(s)	1119
BspTI	CTTAAG	1 cut(s)	1679
BspTNI	GGTCTC	1 cut(s)	2375
BsrDI	GCAATG	1 cut(s)	2062
BsrFI	RCCGGY	2 cut(s)	50, 79
BsrGI	TGTACA	1 cut(s)	982
BsrI	ACTGG	2 cut(s)	518, 1056
BssAI	RCCGGY	2 cut(s)	50, 79
BssECI	CCNNGG	6 cut(s)	124, 540, 733, 1695, 1696, 2353
BssNAI	GTATAC	1 cut(s)	2315
BssSI	CACGAG	1 cut(s)	1133
BssT1I	CCWWGG	3 cut(s)	124, 733, 2353
Bst1107I	GTATAC	1 cut(s)	2315
Bst2BI	CACGAG	1 cut(s)	1133
Bst2UI	CCWGG	3 cut(s)	1697, 2343, 2397
Bst6I	CTCTTC	1 cut(s)	1119
BstAFI	CTTAAG	1 cut(s)	1679
BstAUI	TGTACA	1 cut(s)	982
BstC8I	GCNNGC	6 cut(s)	81, 112, 342, 727, 1324, 2223
BstDEI	CTNAG	5 cut(s)	188, 786, 1385, 1778, 1996
BstDSI	CCRYGG	1 cut(s)	540
BstENI	CCTNNNNNAGG	1 cut(s)	2076
BstF5I	GGATG	8 cut(s)	154, 328, 529, 553, 970, 1170, 1453, 1732
BstFNI	CGCG	1 cut(s)	2626
BstHHI	GCGC	2 cut(s)	50, 1955
BstMAI	GTCTC	5 cut(s)	315, 883, 1388, 2311, 2375
BstMWI	GCNNNNNNNGC	6 cut(s)	20, 193, 735, 1571, 2329, 2338
BstNI	CCWGG	3 cut(s)	1697, 2343, 2397
BstNSI	RCATGY	2 cut(s)	368, 2518
BstSCI	CCNGG	6 cut(s)	9, 96, 331, 1695, 2341, 2395
BstSFI	CTRYAG	4 cut(s)	813, 1434, 2200, 2524
BstSLI	GKGCMC	1 cut(s)	173
BstUI	CGCG	1 cut(s)	2626
BstV1I	GCAGC	5 cut(s)	795, 1141, 1561, 1624, 2529
BstX2I	RGATCY	2 cut(s)	535, 1381
BstYI	RGATCY	2 cut(s)	535, 1381
BstZ17I	GTATAC	1 cut(s)	2315
BsuRI	GGCC	3 cut(s)	340, 1731, 2273
BtgI	CCRYGG	1 cut(s)	540
BtsCI	GGATG	8 cut(s)	154, 328, 529, 553, 970, 1170, 1453, 1732
BtsI	GCAGTG	2 cut(s)	173, 1048
BtsIMutI	CAGTG	2 cut(s)	173, 1048
BtuMI	TCGCGA	1 cut(s)	2626
BveI	ACCTGC	1 cut(s)	2314
Cac8I	GCNNGC	6 cut(s)	81, 112, 342, 727, 1324, 2223
CaiI	CAGNNNCTG	2 cut(s)	1013, 1397
CfoI	GCGC	2 cut(s)	50, 1955
Cfr10I	RCCGGY	2 cut(s)	50, 79
Cfr13I	GGNCC	2 cut(s)	1700, 1940
Csp6I	GTAC	6 cut(s)	362, 488, 983, 1389, 1927, 2445
CspCI	CAANNNNNGTGG	6 cut(s)	771, 806, 1346, 1381, 1419, 1454
CviQI	GTAC	6 cut(s)	362, 488, 983, 1389, 1927, 2445
DdeI	CTNAG	5 cut(s)	188, 786, 1385, 1778, 1996
DrdI	GACNNNNNNGTC	2 cut(s)	1472, 2288
DseDI	GACNNNNNNGTC	2 cut(s)	1472, 2288
EaeI	YGGCCR	2 cut(s)	1729, 2271
Eam1104I	CTCTTC	1 cut(s)	1119
EarI	CTCTTC	1 cut(s)	1119
Ecl136II	GAGCTC	1 cut(s)	187
Eco130I	CCWWGG	3 cut(s)	124, 733, 2353
Eco24I	GRGCYC	1 cut(s)	189
Eco31I	GGTCTC	1 cut(s)	2375
Eco32I	GATATC	4 cut(s)	131, 244, 388, 1582
Eco47I	GGWCC	2 cut(s)	1700, 1940
Eco53kI	GAGCTC	1 cut(s)	187
Eco57I	CTGAAG	1 cut(s)	1143
EcoICRI	GAGCTC	1 cut(s)	187
EcoNI	CCTNNNNNAGG	1 cut(s)	2076
EcoO109I	RGGNCCY	1 cut(s)	1700
EcoRII	CCWGG	3 cut(s)	1695, 2341, 2395
EcoRV	GATATC	4 cut(s)	131, 244, 388, 1582
EcoT14I	CCWWGG	3 cut(s)	124, 733, 2353
EcoT22I	ATGCAT	3 cut(s)	154, 751, 2062
EcoT38I	GRGCYC	1 cut(s)	189
ErhI	CCWWGG	3 cut(s)	124, 733, 2353
Esp3I	CGTCTC	1 cut(s)	883
FaqI	GGGAC	2 cut(s)	1686, 1902
FblI	GTMKAC	3 cut(s)	1965, 2280, 2314
Fnu4HI	GCNGC	5 cut(s)	809, 1130, 1575, 1638, 2543
FokI	GGATG	8 cut(s)	161, 315, 516, 540, 977, 1157, 1460, 1739
FriOI	GRGCYC	1 cut(s)	189
Fsp4HI	GCNGC	5 cut(s)	809, 1130, 1575, 1638, 2543
FspBI	CTAG	6 cut(s)	213, 879, 1379, 1607, 1688, 1853
GlaI	GCGC	2 cut(s)	49, 1954
GluI	GCNGC	5 cut(s)	809, 1130, 1575, 1638, 2543
HaeIII	GGCC	3 cut(s)	340, 1731, 2273
HapII	CCGG	7 cut(s)	11, 51, 80, 97, 333, 533, 1761
HhaI	GCGC	2 cut(s)	50, 1955
Hin6I	GCGC	2 cut(s)	48, 1953
HinP1I	GCGC	2 cut(s)	48, 1953
HincII	GTYRAC	4 cut(s)	357, 1966, 2281, 2467
HindII	GTYRAC	4 cut(s)	357, 1966, 2281, 2467
HindIII	AAGCTT	3 cut(s)	260, 997, 2414
HpaII	CCGG	7 cut(s)	11, 51, 80, 97, 333, 533, 1761
HphI	GGTGA	7 cut(s)	446, 1307, 1463, 1724, 1885, 1985, 2061
Hpy166II	GTNNAC	8 cut(s)	67, 289, 357, 1368, 1966, 2281, 2315, 2467
Hpy8I	GTNNAC	8 cut(s)	67, 289, 357, 1368, 1966, 2281, 2315, 2467
Hpy99I	CGWCG	2 cut(s)	119, 1405
HpyAV	CCTTC	6 cut(s)	302, 1268, 1879, 2108, 2153, 2358
HpyCH4IV	ACGT	2 cut(s)	1403, 2590
HpyF10VI	GCNNNNNNNGC	6 cut(s)	20, 193, 735, 1571, 2329, 2338
HpyF3I	CTNAG	5 cut(s)	188, 786, 1385, 1778, 1996
HpySE526I	ACGT	2 cut(s)	1403, 2590
HspAI	GCGC	2 cut(s)	48, 1953
KflI	GGGWCCC	1 cut(s)	1700
Kpn2I	TCCGGA	1 cut(s)	532
KroI	GCCGGC	1 cut(s)	79
KroNI	GCCGGC	1 cut(s)	81
LguI	GCTCTTC	1 cut(s)	1119
LmnI	GCTCC	3 cut(s)	76, 184, 1562
Lsp1109I	GCAGC	5 cut(s)	795, 1141, 1561, 1624, 2529
LweI	GCATC	7 cut(s)	139, 538, 758, 1000, 1153, 1609, 2547
MaeI	CTAG	6 cut(s)	213, 879, 1379, 1607, 1688, 1853
MaeII	ACGT	2 cut(s)	1403, 2590
MaeIII	GTNAC	8 cut(s)	461, 1295, 1451, 1609, 1900, 1919, 2236, 2326
MboII	GAAGA	9 cut(s)	97, 270, 822, 914, 1136, 1416, 1841, 2094, 2168
MfeI	CAATTG	1 cut(s)	1797
MflI	RGATCY	2 cut(s)	535, 1381
MhlI	GDGCHC	3 cut(s)	173, 189, 1414
MlsI	TGGCCA	2 cut(s)	1731, 2273
MluNI	TGGCCA	2 cut(s)	1731, 2273
MlyI	GAGTC	6 cut(s)	113, 379, 1274, 1693, 1884, 2472
MmeI	TCCRAC	5 cut(s)	1293, 1454, 1456, 1775, 2444
Mox20I	TGGCCA	2 cut(s)	1731, 2273
Mph1103I	ATGCAT	3 cut(s)	154, 751, 2062
MroI	TCCGGA	1 cut(s)	532
MroNI	GCCGGC	1 cut(s)	79
MroXI	GAANNNNTTC	4 cut(s)	92, 600, 880, 1533
MscI	TGGCCA	2 cut(s)	1731, 2273
MseI	TTAA	7 cut(s)	504, 632, 861, 1341, 1362, 1680, 1862
MslI	CAYNNNNRTG	4 cut(s)	558, 1629, 1737, 2109
Msp20I	TGGCCA	2 cut(s)	1731, 2273
MspCI	CTTAAG	1 cut(s)	1679
MspI	CCGG	7 cut(s)	11, 51, 80, 97, 333, 533, 1761
MspR9I	CCNGG	6 cut(s)	11, 98, 333, 1697, 2343, 2397
MunI	CAATTG	1 cut(s)	1797
MvaI	CCWGG	3 cut(s)	1697, 2343, 2397
MvnI	CGCG	1 cut(s)	2626
MwoI	GCNNNNNNNGC	6 cut(s)	20, 193, 735, 1571, 2329, 2338
NaeI	GCCGGC	1 cut(s)	81
NciI	CCSGG	3 cut(s)	11, 98, 333
NgoMIV	GCCGGC	1 cut(s)	79
NlaIV	GGNNCC	5 cut(s)	78, 537, 1701, 1702, 1749
NmuCI	GTSAC	4 cut(s)	1295, 1451, 1609, 2236
NruI	TCGCGA	1 cut(s)	2626
NsiI	ATGCAT	3 cut(s)	154, 751, 2062
NspI	RCATGY	2 cut(s)	368, 2518
PasI	CCCWGGG	1 cut(s)	1696
PciI	ACATGT	1 cut(s)	2514
PciSI	GCTCTTC	1 cut(s)	1119
PcsI	WCGNNNNNNNCGW	1 cut(s)	69
PdiI	GCCGGC	1 cut(s)	81
PdmI	GAANNNNTTC	4 cut(s)	92, 600, 880, 1533
PfeI	GAWTC	8 cut(s)	572, 596, 662, 920, 1016, 1094, 1459, 1840
PfoI	TCCNGGA	2 cut(s)	96, 2395
PkrI	GCNGC	5 cut(s)	810, 1131, 1576, 1639, 2544
PleI	GAGTC	6 cut(s)	113, 378, 1273, 1692, 1884, 2472
PpsI	GAGTC	6 cut(s)	113, 378, 1273, 1692, 1884, 2472
PpuMI	RGGWCCY	1 cut(s)	1700
PscI	ACATGT	1 cut(s)	2514
Psp124BI	GAGCTC	1 cut(s)	189
Psp5II	RGGWCCY	1 cut(s)	1700
Psp6I	CCWGG	3 cut(s)	1695, 2341, 2395
PspGI	CCWGG	3 cut(s)	1695, 2341, 2395
PspN4I	GGNNCC	5 cut(s)	78, 537, 1701, 1702, 1749
PspPI	GGNCC	2 cut(s)	1700, 1940
PspPPI	RGGWCCY	1 cut(s)	1700
PstNI	CAGNNNCTG	2 cut(s)	1013, 1397
PsuI	RGATCY	2 cut(s)	535, 1381
RruI	TCGCGA	1 cut(s)	2626
RsaI	GTAC	6 cut(s)	363, 489, 984, 1390, 1928, 2446
RsaNI	GTAC	6 cut(s)	362, 488, 983, 1389, 1927, 2445
RseI	CAYNNNNRTG	4 cut(s)	558, 1629, 1737, 2109
SacI	GAGCTC	1 cut(s)	189
SalI	GTCGAC	2 cut(s)	1964, 2279
SapI	GCTCTTC	1 cut(s)	1119
SaqAI	TTAA	7 cut(s)	504, 632, 861, 1341, 1362, 1680, 1862
SatI	GCNGC	5 cut(s)	809, 1130, 1575, 1638, 2543
Sau96I	GGNCC	2 cut(s)	1700, 1940
SchI	GAGTC	6 cut(s)	113, 379, 1274, 1693, 1884, 2472
ScrFI	CCNGG	6 cut(s)	11, 98, 333, 1697, 2343, 2397
SduI	GDGCHC	3 cut(s)	173, 189, 1414
SfaNI	GCATC	7 cut(s)	139, 538, 758, 1000, 1153, 1609, 2547
SfcI	CTRYAG	4 cut(s)	813, 1434, 2200, 2524
SinI	GGWCC	2 cut(s)	1700, 1940
SmiMI	CAYNNNNRTG	4 cut(s)	558, 1629, 1737, 2109
SmlI	CTYRAG	3 cut(s)	263, 1679, 2595
SmoI	CTYRAG	3 cut(s)	263, 1679, 2595
SpeI	ACTAGT	2 cut(s)	878, 1606
SsiI	CCGC	2 cut(s)	1193, 1373
SspMI	CTAG	6 cut(s)	213, 879, 1379, 1607, 1688, 1853
SstI	GAGCTC	1 cut(s)	189
StyD4I	CCNGG	6 cut(s)	9, 96, 331, 1695, 2341, 2395
StyI	CCWWGG	3 cut(s)	124, 733, 2353
TaiI	ACGT	2 cut(s)	1406, 2593
TaqI	TCGA	7 cut(s)	56, 390, 1155, 1282, 1347, 1965, 2280
TatI	WGTACW	2 cut(s)	982, 1388
TfiI	GAWTC	8 cut(s)	572, 596, 662, 920, 1016, 1094, 1459, 1840
Tru1I	TTAA	7 cut(s)	504, 632, 861, 1341, 1362, 1680, 1862
Tru9I	TTAA	7 cut(s)	504, 632, 861, 1341, 1362, 1680, 1862
TscAI	CASTG	2 cut(s)	173, 1048
TseFI	GTSAC	4 cut(s)	1295, 1451, 1609, 2236
TseI	GCWGC	5 cut(s)	808, 1129, 1574, 1637, 2542
Tsp45I	GTSAC	4 cut(s)	1295, 1451, 1609, 2236
TspGWI	ACGGA	6 cut(s)	215, 836, 1025, 1397, 2138, 2490
TspRI	CASTG	2 cut(s)	173, 1048
Vha464I	CTTAAG	1 cut(s)	1679
VpaK11BI	GGWCC	2 cut(s)	1700, 1940
XagI	CCTNNNNNAGG	1 cut(s)	2076
XapI	RAATTY	5 cut(s)	5, 778, 1532, 1554, 1662
XceI	RCATGY	2 cut(s)	368, 2518
XmiI	GTMKAC	3 cut(s)	1965, 2280, 2314
XmnI	GAANNNNTTC	4 cut(s)	92, 600, 880, 1533
XspI	CTAG	6 cut(s)	213, 879, 1379, 1607, 1688, 1853
Zsp2I	ATGCAT	3 cut(s)	154, 751, 2062

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.