MD04G1154900.v1.1

DUF761-associated sequence motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011783
Physical Location & Seq
Reverse (-)
24296407 .. 24298287
1881 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1154900.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGAGTGGTAACTCAGTTCATCAGACCTCTGGCTGTTTCAACGGCATCCTGCGCCGGCTTTTGTGCAATGGAAGCGTTCCAACACACCCATCAGATCAAATTCCAGAATCCAACACTACCCAACTCGCAAAATCTCCCATAAAACAAAACCCTGATCCCAAAATTCAAACTTCAGCTTCTGTCACGCCTGGAATCGTGGCAAGGCTGATGGGTTTGGACTCGTTGCCGGAAACAAATTGGGTTTCAAGAGCCCCGGAGTTGGTCAGTCGAAGTAGGTCGTTGAGTTTTGCAGATTACTTGATGGGTTTTGACTCCAATGATCTGCATCGTAGAGTTCGAACCTCGGTTTCTTTTCGTGAAGTTCCGGAGGTTATGCATCGGCAGAAGAATAGCCATGATTTTATGGTTGTGTGTTTGGACAATGTGGGTGAGAAATCGAAGGGAGTTGGGTCGATGGTGAAGAAATCTGAGGTGGGGTTTGGGGAACTGAGGCAGGGGAAGAAGGAACAGAGTAAGAAGAACAAGGTGAAGAATGATGAGATTATGAAGGAGAAGAAGAAGGAAAAAACTGTAATGATAAAGCAAAACAAGAAGATTTCTTGTTTAAAAGATGAGCCCAGAAGGGAATTTGGTGCAAAATCTTCAAAGAGTTACAAAAATGTTTGTGCAAATTCGAAGGGGGAGAGTGGAAATCTTGCATTGAAAAAAGCAACCCAGAAGAAGAAAGTTGTGGTGGAACCAAAAATCAAGAAGAGAAACCATAAAAATGAAGCTGAAAAGGGAAAAGAGGCCTCTGAATTTCATGGCTCCTCAGAGAGCGATGCAAGCCCTGTTTCAGTTCTTGACATTGATGACATGTTGATCGAGCATGAAGCTTGGTTATCAGGATATTCAATTCCCATGGATTTGAACTCCACTGTGAAACCGTTACCAAAAGCTCCTTTTAGTGACAGCTCAGAAGTGAAGGCAGCAAAAAGGAAAGATTTTGAGCCGATTAACGAAGAGGAAACCAGAGACTACGCAAAGCTGCTGGCTTCCAAAATCCAAAATTGGACGGAGGTGGATTTGAAAGAATCAAACTGGGTGGCCAAGAATGTGGTCGGGTTCGAAGGTTTCGAAGAAATCTGCGAGGAATTTGAAGGGCGGATCCTTGACACGCTATTGCACCAAGCCATTGATGAATTTATTGAGATTTCTTATGAAAATGTGTGGGGAAATCTGTGGACTTGCAATGCTGCTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VARLMGL

Protein Analysis

415

Amino Acids

46.41

Weight (kDa)

8.61

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VARLMGL PF14383 62 - 79 4.4e-08 DUF761-associated sequence motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011783)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01370
fragaria_vesca FvH4_6g11320 FvH4_6g11320
malus_domestica MD04G1154900.v1.1 MD12G1168500.v1.1
prunus_persica Prupe.6G278700_v2.0.a1
pyrus_communis pycom04g13970 pycom12g16000
rosa_chinensis RchiOBHm_Chr3g0462881
rosa_laevigata RLG00000024826
rosa_multiflora Rmu_sc0000766.1_g000014 Rmu_sc0000766.1_g000015
rosa_roxburghii Rroxscaffold_6G00417130
rosa_rugosa Rorug03G0059600
rosa_samantha Rh3AG117900 Rh3BG121700 Rh3CG124600 Rh3DG123300
rosa_wichuraiana Rw3G010000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 364
AciI CCGC 1 cut(s) 1144
AclWI GGATC 3 cut(s) 149, 1141, 1154
AcoI YGGCCR 1 cut(s) 1086
AcsI RAATTY 7 cut(s) 99, 162, 626, 670, 797, 1133, 1181
AcuI CTGAAG 1 cut(s) 156
AfiI CCNNNNNNNGG 1 cut(s) 259
AflIII ACRYGT 1 cut(s) 855
AgsI TTSAA 9 cut(s) 40, 167, 246, 645, 703, 894, 910, 1069, 1139
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 6 cut(s) 176, 773, 875, 938, 954, 1027
AluI AGCT 6 cut(s) 176, 773, 875, 938, 954, 1027
Alw26I GTCTC 1 cut(s) 1008
AlwI GGATC 3 cut(s) 149, 1141, 1154
AlwNI CAGNNNCTG 1 cut(s) 179
Aor13HI TCCGGA 1 cut(s) 364
AoxI GGCC 2 cut(s) 789, 1086
ApeKI GCWGC 3 cut(s) 968, 1027, 1235
ApoI RAATTY 7 cut(s) 99, 162, 626, 670, 797, 1133, 1181
AspLEI GCGC 1 cut(s) 54
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 3 cut(s) 440, 469, 538
AsuII TTCGAA 4 cut(s) 337, 674, 1107, 1116
BalI TGGCCA 1 cut(s) 1088
BamHI GGATCC 1 cut(s) 1146
BanII GRGCYC 2 cut(s) 253, 618
BbvI GCAGC 3 cut(s) 980, 1014, 1222
BccI CCATC 4 cut(s) 97, 202, 295, 448
BceAI ACGGC 1 cut(s) 58
BciT130I CCWGG 1 cut(s) 189
BcnI CCSGG 1 cut(s) 254
BcoDI GTCTC 1 cut(s) 1008
BfaI CTAG 1 cut(s) 1239
BisI GCNGC 3 cut(s) 969, 1028, 1236
BlsI GCNGC 3 cut(s) 970, 1029, 1237
Bme1390I CCNGG 2 cut(s) 189, 254
BmiI GGNNCC 3 cut(s) 738, 808, 1148
BmrFI CCNGG 2 cut(s) 189, 254
BmrI ACTGGG 1 cut(s) 1090
BmsI GCATC 4 cut(s) 54, 334, 385, 811
BmuI ACTGGG 1 cut(s) 1090
Bpu14I TTCGAA 4 cut(s) 337, 674, 1107, 1116
BpuMI CCSGG 1 cut(s) 254
BsaBI GATNNNNATC 1 cut(s) 324
BsaJI CCNNGG 3 cut(s) 252, 342, 900
BsaWI WCCGGW 1 cut(s) 364
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse118I RCCGGY 1 cut(s) 54
Bse1I ACTGG 1 cut(s) 1085
Bse3DI GCAATG 2 cut(s) 73, 1237
Bse8I GATNNNNATC 1 cut(s) 324
BseAI TCCGGA 1 cut(s) 364
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 3 cut(s) 252, 342, 900
BseGI GGATG 1 cut(s) 45
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMI GCAATG 2 cut(s) 73, 1237
BseMII CTCAG 5 cut(s) 27, 459, 479, 825, 969
BseNI ACTGG 1 cut(s) 1085
BseRI GAGGAG 1 cut(s) 799
BseXI GCAGC 3 cut(s) 980, 1014, 1222
BshFI GGCC 2 cut(s) 791, 1088
BsiSI CCGG 4 cut(s) 55, 227, 254, 365
BslI CCNNNNNNNGG 1 cut(s) 259
BsmAI GTCTC 1 cut(s) 1008
BsnI GGCC 2 cut(s) 791, 1088
Bsp119I TTCGAA 4 cut(s) 337, 674, 1107, 1116
Bsp1286I GDGCHC 2 cut(s) 253, 618
Bsp13I TCCGGA 1 cut(s) 364
Bsp143I GATC 5 cut(s) 94, 154, 319, 861, 1146
Bsp19I CCATGG 1 cut(s) 900
BspACI CCGC 1 cut(s) 1144
BspANI GGCC 2 cut(s) 791, 1088
BspCNI CTCAG 5 cut(s) 26, 460, 480, 824, 968
BspEI TCCGGA 1 cut(s) 364
BspLI GGNNCC 3 cut(s) 738, 808, 1148
BspPI GGATC 3 cut(s) 149, 1141, 1154
BspT104I TTCGAA 4 cut(s) 337, 674, 1107, 1116
BsrDI GCAATG 2 cut(s) 73, 1237
BsrFI RCCGGY 1 cut(s) 54
BsrI ACTGG 1 cut(s) 1085
BssAI RCCGGY 1 cut(s) 54
BssECI CCNNGG 3 cut(s) 252, 342, 900
BssMI GATC 5 cut(s) 94, 154, 319, 861, 1146
BssT1I CCWWGG 1 cut(s) 900
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 3 cut(s) 571, 919, 927
Bst6I CTCTTC 2 cut(s) 746, 996
BstBI TTCGAA 4 cut(s) 337, 674, 1107, 1116
BstC8I GCNNGC 3 cut(s) 56, 826, 1032
BstDEI CTNAG 5 cut(s) 13, 468, 488, 811, 955
BstDSI CCRYGG 1 cut(s) 900
BstF5I GGATG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 54
BstKTI GATC 5 cut(s) 97, 157, 322, 864, 1149
BstMAI GTCTC 1 cut(s) 1008
BstMBI GATC 5 cut(s) 94, 154, 319, 861, 1146
BstMWI GCNNNNNNNGC 3 cut(s) 51, 72, 825
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 1 cut(s) 859
BstSCI CCNGG 2 cut(s) 187, 252
BstV1I GCAGC 3 cut(s) 980, 1014, 1222
BstX2I RGATCY 1 cut(s) 1146
BstXI CCANNNNNNTGG 1 cut(s) 1096
BstYI RGATCY 1 cut(s) 1146
BsuRI GGCC 2 cut(s) 791, 1088
BtgI CCRYGG 1 cut(s) 900
BtgZI GCGATG 1 cut(s) 834
BtsCI GGATG 1 cut(s) 45
BtsIMutI CAGTG 1 cut(s) 915
Cac8I GCNNGC 3 cut(s) 56, 826, 1032
CaiI CAGNNNCTG 1 cut(s) 179
CfoI GCGC 1 cut(s) 54
Cfr10I RCCGGY 1 cut(s) 54
CspCI CAANNNNNGTGG 2 cut(s) 1065, 1100
CviAII CATG 5 cut(s) 395, 803, 856, 869, 901
DdeI CTNAG 5 cut(s) 13, 468, 488, 811, 955
DpnI GATC 5 cut(s) 96, 156, 321, 863, 1148
DpnII GATC 5 cut(s) 94, 154, 319, 861, 1146
DraI TTTAAA 1 cut(s) 606
EaeI YGGCCR 1 cut(s) 1086
Eam1104I CTCTTC 2 cut(s) 746, 996
EarI CTCTTC 2 cut(s) 746, 996
EciI GGCGGA 1 cut(s) 1159
Eco130I CCWWGG 1 cut(s) 900
Eco147I AGGCCT 1 cut(s) 791
Eco24I GRGCYC 2 cut(s) 253, 618
Eco57I CTGAAG 1 cut(s) 156
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 900
EcoT22I ATGCAT 1 cut(s) 378
EcoT38I GRGCYC 2 cut(s) 253, 618
ErhI CCWWGG 1 cut(s) 900
FaeI CATG 5 cut(s) 398, 806, 859, 872, 904
FatI CATG 5 cut(s) 394, 802, 855, 868, 900
Fnu4HI GCNGC 3 cut(s) 969, 1028, 1236
FokI GGATG 1 cut(s) 32
FriOI GRGCYC 2 cut(s) 253, 618
Fsp4HI GCNGC 3 cut(s) 969, 1028, 1236
FspBI CTAG 1 cut(s) 1239
GlaI GCGC 1 cut(s) 53
GluI GCNGC 3 cut(s) 969, 1028, 1236
HaeIII GGCC 2 cut(s) 791, 1088
HapII CCGG 4 cut(s) 55, 227, 254, 365
HhaI GCGC 1 cut(s) 54
Hin1II CATG 5 cut(s) 398, 806, 859, 872, 904
Hin6I GCGC 1 cut(s) 52
HinP1I GCGC 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 873
HinfI GANTC 5 cut(s) 107, 192, 218, 311, 1073
HpaII CCGG 4 cut(s) 55, 227, 254, 365
HphI GGTGA 3 cut(s) 440, 469, 538
Hpy166II GTNNAC 1 cut(s) 1224
Hpy188I TCNGA 6 cut(s) 24, 94, 469, 796, 814, 958
Hpy188III TCNNGA 7 cut(s) 104, 246, 356, 365, 748, 842, 885
Hpy8I GTNNAC 1 cut(s) 1224
HpyAV CCTTC 9 cut(s) 433, 496, 541, 553, 615, 670, 958, 1103, 1133
HpyCH4III ACNGT 3 cut(s) 571, 919, 927
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 72, 825
HpyF3I CTNAG 5 cut(s) 13, 468, 488, 811, 955
Hsp92II CATG 5 cut(s) 398, 806, 859, 872, 904
HspAI GCGC 1 cut(s) 52
Kpn2I TCCGGA 1 cut(s) 364
KroI GCCGGC 1 cut(s) 54
KroNI GCCGGC 1 cut(s) 56
Kzo9I GATC 5 cut(s) 94, 154, 319, 861, 1146
LmnI GCTCC 2 cut(s) 812, 943
Lsp1109I GCAGC 3 cut(s) 980, 1014, 1222
LweI GCATC 4 cut(s) 54, 334, 385, 811
MaeI CTAG 1 cut(s) 1239
MaeIII GTNAC 5 cut(s) 8, 181, 650, 927, 947
MalI GATC 5 cut(s) 96, 156, 321, 863, 1148
MboI GATC 5 cut(s) 94, 154, 319, 861, 1146
MflI RGATCY 1 cut(s) 1146
MhlI GDGCHC 2 cut(s) 253, 618
MlsI TGGCCA 1 cut(s) 1088
MluNI TGGCCA 1 cut(s) 1088
MlyI GAGTC 2 cut(s) 212, 305
MmeI TCCRAC 2 cut(s) 104, 135
Mox20I TGGCCA 1 cut(s) 1088
Mph1103I ATGCAT 1 cut(s) 378
MroI TCCGGA 1 cut(s) 364
MroNI GCCGGC 1 cut(s) 54
MscI TGGCCA 1 cut(s) 1088
MseI TTAA 3 cut(s) 605, 996, 1243
MslI CAYNNNNRTG 1 cut(s) 765
Msp20I TGGCCA 1 cut(s) 1088
MspI CCGG 4 cut(s) 55, 227, 254, 365
MspR9I CCNGG 2 cut(s) 189, 254
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 3 cut(s) 51, 72, 825
NaeI GCCGGC 1 cut(s) 56
NciI CCSGG 1 cut(s) 254
NcoI CCATGG 1 cut(s) 900
NdeII GATC 5 cut(s) 94, 154, 319, 861, 1146
NgoMIV GCCGGC 1 cut(s) 54
NlaIII CATG 5 cut(s) 398, 806, 859, 872, 904
NlaIV GGNNCC 3 cut(s) 738, 808, 1148
NmuCI GTSAC 2 cut(s) 181, 947
NsiI ATGCAT 1 cut(s) 378
NspI RCATGY 1 cut(s) 859
NspV TTCGAA 4 cut(s) 337, 674, 1107, 1116
PceI AGGCCT 1 cut(s) 791
PciI ACATGT 1 cut(s) 855
PcsI WCGNNNNNNNCGW 2 cut(s) 334, 1113
PdiI GCCGGC 1 cut(s) 56
PfeI GAWTC 3 cut(s) 107, 192, 1073
PkrI GCNGC 3 cut(s) 970, 1029, 1237
PleI GAGTC 2 cut(s) 212, 305
PpsI GAGTC 2 cut(s) 212, 305
PscI ACATGT 1 cut(s) 855
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 3 cut(s) 738, 808, 1148
PstNI CAGNNNCTG 1 cut(s) 179
PsuI RGATCY 1 cut(s) 1146
RseI CAYNNNNRTG 1 cut(s) 765
SaqAI TTAA 3 cut(s) 605, 996, 1243
SatI GCNGC 3 cut(s) 969, 1028, 1236
Sau3AI GATC 5 cut(s) 94, 154, 319, 861, 1146
SchI GAGTC 2 cut(s) 212, 305
ScrFI CCNGG 2 cut(s) 189, 254
SduI GDGCHC 2 cut(s) 253, 618
SfaNI GCATC 4 cut(s) 54, 334, 385, 811
SfuI TTCGAA 4 cut(s) 337, 674, 1107, 1116
SmiMI CAYNNNNRTG 1 cut(s) 765
SseBI AGGCCT 1 cut(s) 791
SsiI CCGC 1 cut(s) 1144
SspMI CTAG 1 cut(s) 1239
StuI AGGCCT 1 cut(s) 791
StyD4I CCNGG 2 cut(s) 187, 252
StyI CCWWGG 1 cut(s) 900
TaaI ACNGT 3 cut(s) 571, 919, 927
TaqI TCGA 8 cut(s) 268, 337, 437, 452, 674, 864, 1107, 1116
TfiI GAWTC 3 cut(s) 107, 192, 1073
Tru1I TTAA 3 cut(s) 605, 996, 1243
Tru9I TTAA 3 cut(s) 605, 996, 1243
TscAI CASTG 1 cut(s) 922
TseFI GTSAC 2 cut(s) 181, 947
TseI GCWGC 3 cut(s) 968, 1027, 1235
Tsp45I GTSAC 2 cut(s) 181, 947
TspDTI ATGAA 7 cut(s) 8, 560, 783, 791, 885, 1194, 1215
TspGWI ACGGA 1 cut(s) 1070
TspRI CASTG 1 cut(s) 922
XapI RAATTY 7 cut(s) 99, 162, 626, 670, 797, 1133, 1181
XceI RCATGY 1 cut(s) 859
XspI CTAG 1 cut(s) 1239
Zsp2I ATGCAT 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.