MD12G1168500.v1.1

DUF761-associated sequence motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011783
Physical Location & Seq
Reverse (-)
24889893 .. 24891868
1976 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1168500.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGAGTAGCAACTCAGTTCATCAGAACTCCGGCTGCTTCAATATCATCCTGCGCAGGCTTCTGTGCAATGGAAGCGTTCCGACTCATCCATCAGACCAAATTTCAGATTCTAACACAACCCAGCTCGCAAAACCCCCAAGAAAACAAAACCCTGATCCCAAAATTCAGTCTTCAGCTTCTGTCACGCCTGGAATCGTGGCAAGGCTGATGGGTTTGGACTCGTTGCCGGAAACAAATTGGGTTTCAAGAGGCCCGGACTTGGTCAGTCGGAGTAGGTCACTGAGTTTTGCAGATTACTTGATGGATTTCGACTCGAATGATCATCAGAATCACCAGCATCGAAGGGTTCGAACTTCGGTCTCTTTTCGCGAAGTCCCGGAGGTAATGAATGGTCAGAAGAGCAGCCATGATTTTGTGGTTGTGTATTTGGACAATGTGGGTGAGAAATCGAAGGAAGTTGGGTCGAAGGTGAAGAAATCCGAGTTGAGTTTTGGGGAGTTGAGGCAGGGGAAGAAGGAACAGAGGAACAAGAACAAGGAGAAAAACAGAGAGATTGGTGAGATTATGAAGGAGGAGAAGAAGGAGAAAATAGTAATGATAAAGCAAAACAAGAACATTTGTAGGTTAAAAGATGAGCCCAGAAGGGATTTTGGTGCCAAATCTTCAAAGGGTTCTAAAAATGGTGGTGCAAATTTGAAGGGGGAGAGAGAAAATATTGCAATGAAGAAGACAACCCAGAAGAAGAAAGTTGTAGTGGAACCAAGAATCAAGAAGAGAAAGCAGAAAATTGAAGCTGAAAAGGAAAAAGTGGCTGCAGATTTTCATGGTTCCTCAGAGAATGATACAAGTGTTGCTTCTGTTCTTGGCATTGATGACATTTTGATCGAGCATGAAGCTTGGTTATCAGGATATTCAATTCCCATGGATTTGAACTCTAAGGTGAAACCGTCGCCAAAAGCTCCATTTAGTGACAGATCAGAAGTGAACGCAGCTAAAAGGAAAGATTTTGAGCCGATTAGGGAGGAGGAAACCAGAGACTACGCAGAGCTGCTGGCTTCCAAAATCCAAAATTGGATAGAGGAGGATTTGAAAGAATCAAATTGGGTGGCCAAGAATGCGGTCGGGTTCGAAGGTTTCGAAGAAATCTGCGAGGAATTTGAAGGGCGGATTCTGGACATGCTAGTGCACCAGACCATTGATGAATGTATTGAGATTTCTTATGAAAATATATGGGGAAATCTGTGGACTTGCAATGCTACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VARLMGL

Protein Analysis

422

Amino Acids

47.72

Weight (kDa)

8.33

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VARLMGL PF14383 62 - 79 3.5e-08 DUF761-associated sequence motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011783)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01370
fragaria_vesca FvH4_6g11320 FvH4_6g11320
malus_domestica MD04G1154900.v1.1 MD12G1168500.v1.1
prunus_persica Prupe.6G278700_v2.0.a1
pyrus_communis pycom04g13970 pycom12g16000
rosa_chinensis RchiOBHm_Chr3g0462881
rosa_laevigata RLG00000024826
rosa_multiflora Rmu_sc0000766.1_g000014 Rmu_sc0000766.1_g000015
rosa_roxburghii Rroxscaffold_6G00417130
rosa_rugosa Rorug03G0059600
rosa_samantha Rh3AG117900 Rh3BG121700 Rh3CG124600 Rh3DG123300
rosa_wichuraiana Rw3G010000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 53
AccB1I GGYRCC 1 cut(s) 653
AccII CGCG 1 cut(s) 369
AciI CCGC 2 cut(s) 1118, 1165
AclWI GGATC 1 cut(s) 149
AcoI YGGCCR 1 cut(s) 1107
AcsI RAATTY 4 cut(s) 99, 162, 691, 1154
AcuI CTGAAG 1 cut(s) 156
AfiI CCNNNNNNNGG 1 cut(s) 259
AgsI TTSAA 9 cut(s) 40, 246, 666, 697, 791, 915, 931, 1090, 1160
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 7 cut(s) 124, 176, 794, 896, 959, 992, 1048
AluI AGCT 7 cut(s) 124, 176, 794, 896, 959, 992, 1048
Alw21I GWGCWC 1 cut(s) 1188
Alw26I GTCTC 2 cut(s) 364, 1029
Alw44I GTGCAC 1 cut(s) 1184
AlwI GGATC 1 cut(s) 149
AlwNI CAGNNNCTG 1 cut(s) 179
AoxI GGCC 2 cut(s) 250, 1107
ApaLI GTGCAC 1 cut(s) 1184
ApeKI GCWGC 5 cut(s) 33, 402, 812, 989, 1048
ApoI RAATTY 4 cut(s) 99, 162, 691, 1154
AspLEI GCGC 1 cut(s) 54
AspS9I GGNCC 1 cut(s) 251
AsuC2I CCSGG 2 cut(s) 254, 377
AsuHPI GGTGA 5 cut(s) 323, 452, 481, 569, 952
AsuII TTCGAA 3 cut(s) 349, 1128, 1137
BaeGI GKGCMC 1 cut(s) 1188
BaeI ACNNNNGTAYC 2 cut(s) 834, 867
BalI TGGCCA 1 cut(s) 1109
BanI GGYRCC 1 cut(s) 653
BanII GRGCYC 1 cut(s) 639
BbsI GAAGAC 2 cut(s) 162, 734
Bbv12I GWGCWC 1 cut(s) 1188
BbvI GCAGC 5 cut(s) 20, 414, 799, 1001, 1035
BccI CCATC 3 cut(s) 97, 202, 295
BciT130I CCWGG 1 cut(s) 189
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 2 cut(s) 254, 377
BcoDI GTCTC 2 cut(s) 364, 1029
BfaI CTAG 1 cut(s) 1181
BfmI CTRYAG 1 cut(s) 813
BisI GCNGC 5 cut(s) 34, 403, 813, 990, 1049
BlsI GCNGC 5 cut(s) 35, 404, 814, 991, 1050
Bme1390I CCNGG 3 cut(s) 189, 254, 377
BmgT120I GGNCC 1 cut(s) 251
BmiI GGNNCC 3 cut(s) 655, 759, 829
BmrFI CCNGG 3 cut(s) 189, 254, 377
BmsI GCATC 1 cut(s) 346
BpiI GAAGAC 2 cut(s) 162, 734
Bpu14I TTCGAA 3 cut(s) 349, 1128, 1137
BpuMI CCSGG 2 cut(s) 254, 377
BsaI GGTCTC 1 cut(s) 364
BsaJI CCNNGG 1 cut(s) 921
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse3DI GCAATG 3 cut(s) 73, 726, 1258
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 921
BseGI GGATG 2 cut(s) 45, 85
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMI GCAATG 3 cut(s) 73, 726, 1258
BseMII CTCAG 3 cut(s) 27, 272, 846
BseRI GAGGAG 3 cut(s) 587, 1037, 1094
BseSI GKGCMC 1 cut(s) 1188
BseXI GCAGC 5 cut(s) 20, 414, 799, 1001, 1035
BseYI CCCAGC 1 cut(s) 120
Bsh1236I CGCG 1 cut(s) 369
Bsh1285I CGRYCG 1 cut(s) 1122
BshFI GGCC 2 cut(s) 252, 1109
BshNI GGYRCC 1 cut(s) 653
BsiEI CGRYCG 1 cut(s) 1122
BsiHKAI GWGCWC 1 cut(s) 1188
BsiSI CCGG 4 cut(s) 30, 227, 254, 377
BslFI GGGAC 1 cut(s) 359
BslI CCNNNNNNNGG 1 cut(s) 259
BsmAI GTCTC 2 cut(s) 364, 1029
BsmFI GGGAC 1 cut(s) 359
BsmI GAATGC 1 cut(s) 1120
BsnI GGCC 2 cut(s) 252, 1109
Bso31I GGTCTC 1 cut(s) 364
Bsp119I TTCGAA 3 cut(s) 349, 1128, 1137
Bsp1286I GDGCHC 2 cut(s) 639, 1188
Bsp143I GATC 4 cut(s) 154, 319, 882, 974
Bsp19I CCATGG 1 cut(s) 921
Bsp68I TCGCGA 1 cut(s) 369
BspACI CCGC 2 cut(s) 1118, 1165
BspANI GGCC 2 cut(s) 252, 1109
BspCNI CTCAG 3 cut(s) 26, 273, 845
BspFNI CGCG 1 cut(s) 369
BspLI GGNNCC 3 cut(s) 655, 759, 829
BspMAI CTGCAG 1 cut(s) 817
BspPI GGATC 1 cut(s) 149
BspQI GCTCTTC 1 cut(s) 392
BspT104I TTCGAA 3 cut(s) 349, 1128, 1137
BspT107I GGYRCC 1 cut(s) 653
BspTNI GGTCTC 1 cut(s) 364
BsrDI GCAATG 3 cut(s) 73, 726, 1258
BssECI CCNNGG 1 cut(s) 921
BssMI GATC 4 cut(s) 154, 319, 882, 974
BssT1I CCWWGG 1 cut(s) 921
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 1 cut(s) 948
Bst6I CTCTTC 2 cut(s) 392, 767
BstBI TTCGAA 3 cut(s) 349, 1128, 1137
BstC8I GCNNGC 3 cut(s) 56, 126, 1053
BstDEI CTNAG 4 cut(s) 13, 281, 832, 936
BstDSI CCRYGG 1 cut(s) 921
BstF5I GGATG 2 cut(s) 45, 85
BstFNI CGCG 1 cut(s) 369
BstHHI GCGC 1 cut(s) 54
BstKTI GATC 4 cut(s) 157, 322, 885, 977
BstMAI GTCTC 2 cut(s) 364, 1029
BstMBI GATC 4 cut(s) 154, 319, 882, 974
BstMCI CGRYCG 1 cut(s) 1122
BstMWI GCNNNNNNNGC 2 cut(s) 72, 1115
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 1 cut(s) 1180
BstSCI CCNGG 3 cut(s) 187, 252, 375
BstSFI CTRYAG 1 cut(s) 813
BstSLI GKGCMC 1 cut(s) 1188
BstUI CGCG 1 cut(s) 369
BstV1I GCAGC 5 cut(s) 20, 414, 799, 1001, 1035
BstV2I GAAGAC 2 cut(s) 162, 734
BsuRI GGCC 2 cut(s) 252, 1109
BtgI CCRYGG 1 cut(s) 921
BtsCI GGATG 2 cut(s) 45, 85
BtsIMutI CAGTG 1 cut(s) 278
BtuMI TCGCGA 1 cut(s) 369
Cac8I GCNNGC 3 cut(s) 56, 126, 1053
CaiI CAGNNNCTG 1 cut(s) 179
CfoI GCGC 1 cut(s) 54
Cfr13I GGNCC 1 cut(s) 251
CspCI CAANNNNNGTGG 2 cut(s) 1086, 1121
CviAII CATG 5 cut(s) 407, 824, 890, 922, 1177
DdeI CTNAG 4 cut(s) 13, 281, 832, 936
DpnI GATC 4 cut(s) 156, 321, 884, 976
DpnII GATC 4 cut(s) 154, 319, 882, 974
EaeI YGGCCR 1 cut(s) 1107
Eam1104I CTCTTC 2 cut(s) 392, 767
EarI CTCTTC 2 cut(s) 392, 767
EciI GGCGGA 1 cut(s) 1180
Eco130I CCWWGG 1 cut(s) 921
Eco24I GRGCYC 1 cut(s) 639
Eco31I GGTCTC 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 156
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 921
EcoT38I GRGCYC 1 cut(s) 639
ErhI CCWWGG 1 cut(s) 921
FaeI CATG 5 cut(s) 410, 827, 893, 925, 1180
FaiI YATR 9 cut(s) 408, 566, 825, 891, 923, 1178, 1221, 1229, 1231
FalI AAGNNNNNCTT 2 cut(s) 838, 870
FaqI GGGAC 1 cut(s) 359
FatI CATG 5 cut(s) 406, 823, 889, 921, 1176
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 5 cut(s) 34, 403, 813, 990, 1049
FokI GGATG 2 cut(s) 32, 72
FriOI GRGCYC 1 cut(s) 639
Fsp4HI GCNGC 5 cut(s) 34, 403, 813, 990, 1049
FspBI CTAG 1 cut(s) 1181
FspI TGCGCA 1 cut(s) 53
GlaI GCGC 1 cut(s) 53
GluI GCNGC 5 cut(s) 34, 403, 813, 990, 1049
GsaI CCCAGC 1 cut(s) 124
HaeIII GGCC 2 cut(s) 252, 1109
HapII CCGG 4 cut(s) 30, 227, 254, 377
HhaI GCGC 1 cut(s) 54
Hin1II CATG 5 cut(s) 410, 827, 893, 925, 1180
Hin6I GCGC 1 cut(s) 52
HinP1I GCGC 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 894
HinfI GANTC 9 cut(s) 82, 107, 192, 218, 311, 328, 765, 1094, 1168
HpaII CCGG 4 cut(s) 30, 227, 254, 377
HphI GGTGA 5 cut(s) 323, 452, 481, 569, 952
Hpy166II GTNNAC 3 cut(s) 985, 1186, 1245
Hpy188III TCNNGA 5 cut(s) 246, 368, 769, 906, 1172
Hpy8I GTNNAC 3 cut(s) 985, 1186, 1245
Hpy99I CGWCG 1 cut(s) 952
HpyCH4III ACNGT 1 cut(s) 948
HpyCH4V TGCA 7 cut(s) 66, 290, 689, 719, 815, 1186, 1251
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 1115
HpyF3I CTNAG 4 cut(s) 13, 281, 832, 936
Hsp92II CATG 5 cut(s) 410, 827, 893, 925, 1180
HspAI GCGC 1 cut(s) 52
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 4 cut(s) 154, 319, 882, 974
LguI GCTCTTC 1 cut(s) 392
LmnI GCTCC 1 cut(s) 964
Lsp1109I GCAGC 5 cut(s) 20, 414, 799, 1001, 1035
LweI GCATC 1 cut(s) 346
MaeI CTAG 1 cut(s) 1181
MaeIII GTNAC 3 cut(s) 181, 276, 968
MalI GATC 4 cut(s) 156, 321, 884, 976
MboI GATC 4 cut(s) 154, 319, 882, 974
MhlI GDGCHC 2 cut(s) 639, 1188
MlsI TGGCCA 1 cut(s) 1109
MluCI AATT 9 cut(s) 99, 162, 235, 691, 786, 915, 1069, 1099, 1154
MluNI TGGCCA 1 cut(s) 1109
MlyI GAGTC 3 cut(s) 76, 212, 305
MmeI TCCRAC 2 cut(s) 104, 248
Mox20I TGGCCA 1 cut(s) 1109
MscI TGGCCA 1 cut(s) 1109
MseI TTAA 1 cut(s) 626
MslI CAYNNNNRTG 1 cut(s) 1181
Msp20I TGGCCA 1 cut(s) 1109
MspI CCGG 4 cut(s) 30, 227, 254, 377
MspR9I CCNGG 3 cut(s) 189, 254, 377
Mva1269I GAATGC 1 cut(s) 1120
MvaI CCWGG 1 cut(s) 189
MvnI CGCG 1 cut(s) 369
MwoI GCNNNNNNNGC 2 cut(s) 72, 1115
NciI CCSGG 2 cut(s) 254, 377
NcoI CCATGG 1 cut(s) 921
NdeII GATC 4 cut(s) 154, 319, 882, 974
NlaIII CATG 5 cut(s) 410, 827, 893, 925, 1180
NlaIV GGNNCC 3 cut(s) 655, 759, 829
NmuCI GTSAC 3 cut(s) 181, 276, 968
NruI TCGCGA 1 cut(s) 369
NsbI TGCGCA 1 cut(s) 53
NspI RCATGY 1 cut(s) 1180
NspV TTCGAA 3 cut(s) 349, 1128, 1137
PciSI GCTCTTC 1 cut(s) 392
PcsI WCGNNNNNNNCGW 2 cut(s) 346, 1134
PctI GAATGC 1 cut(s) 1120
PfeI GAWTC 6 cut(s) 107, 192, 328, 765, 1094, 1168
PflFI GACNNNGTC 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 5 cut(s) 35, 404, 814, 991, 1050
PleI GAGTC 3 cut(s) 76, 212, 305
PpsI GAGTC 3 cut(s) 76, 212, 305
Psp6I CCWGG 1 cut(s) 187
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 3 cut(s) 655, 759, 829
PspPI GGNCC 1 cut(s) 251
PstI CTGCAG 1 cut(s) 817
PstNI CAGNNNCTG 1 cut(s) 179
PsyI GACNNNGTC 1 cut(s) 260
RruI TCGCGA 1 cut(s) 369
RseI CAYNNNNRTG 1 cut(s) 1181
SapI GCTCTTC 1 cut(s) 392
SaqAI TTAA 1 cut(s) 626
SatI GCNGC 5 cut(s) 34, 403, 813, 990, 1049
Sau3AI GATC 4 cut(s) 154, 319, 882, 974
Sau96I GGNCC 1 cut(s) 251
SchI GAGTC 3 cut(s) 76, 212, 305
ScrFI CCNGG 3 cut(s) 189, 254, 377
SduI GDGCHC 2 cut(s) 639, 1188
SfaNI GCATC 1 cut(s) 346
SfcI CTRYAG 1 cut(s) 813
SfuI TTCGAA 3 cut(s) 349, 1128, 1137
SmiMI CAYNNNNRTG 1 cut(s) 1181
Sse9I AATT 9 cut(s) 99, 162, 235, 691, 786, 915, 1069, 1099, 1154
SsiI CCGC 2 cut(s) 1118, 1165
SspI AATATT 1 cut(s) 715
SspMI CTAG 1 cut(s) 1181
StyD4I CCNGG 3 cut(s) 187, 252, 375
StyI CCWWGG 1 cut(s) 921
TaaI ACNGT 1 cut(s) 948
TaqI TCGA 9 cut(s) 309, 314, 340, 349, 449, 464, 885, 1128, 1137
TaqII GACCGA 1 cut(s) 346
TasI AATT 9 cut(s) 99, 162, 235, 691, 786, 915, 1069, 1099, 1154
TfiI GAWTC 6 cut(s) 107, 192, 328, 765, 1094, 1168
Tru1I TTAA 1 cut(s) 626
Tru9I TTAA 1 cut(s) 626
TscAI CASTG 1 cut(s) 285
TseFI GTSAC 3 cut(s) 181, 276, 968
TseI GCWGC 5 cut(s) 33, 402, 812, 989, 1048
Tsp45I GTSAC 3 cut(s) 181, 276, 968
TspDTI ATGAA 8 cut(s) 8, 401, 581, 737, 812, 906, 1215, 1236
TspRI CASTG 1 cut(s) 285
Tth111I GACNNNGTC 1 cut(s) 260
VneI GTGCAC 1 cut(s) 1184
XapI RAATTY 4 cut(s) 99, 162, 691, 1154
XceI RCATGY 1 cut(s) 1180
XspI CTAG 1 cut(s) 1181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.