MD04G1168500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016874
Physical Location & Seq
Forward (+)
25869980 .. 25870588
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1168500.v1.1.491

Sequence Viewer

Length: 609 bp
ATGACAAAAAAAAAGAGTTCGTCCTCAGAGAAATGCAATTCTTCAGGGAAGCCAGTAAGAAGAAGGTTTACCGCTAAAAGAGGTTCCAAAACACGTGACGTCGATGCTGCTGAAATCAGGATGGAGATTCTCCATGCCATACAACTCCATAGATCATCATCATCATCATCATCATCAGCGGTACCAGAAGCGTCGGCGCCACCACCACCTGTATATGTGGGTCCACAGTTTGATTCATTGGCGGAGCCTATGCCTCTGCCTGGCCCAGTGTGGTCTACAACTACACCTTCAGTATTGCCTTATGCTCCTTCTTCAGCAGCCATGGAAGCCTTGGAATTTGAGTGGGGGGAGAGCCAAGCTGCTTCATATTCTTGGTGGCTAGGGTTTCTGAAGACTTTAGATGGCAAAAATGCTGACAAGTCAAACTATCCTTTGCCATTGGGGAGTTCTAATTGGTTGTTTGGTCAGAACCAGAATAGTAACTGTAAAGTAGATGATGATCAGGACACAGTTGCTTTGGTTGATGCAAATGATCAGAGCCTTTCACCAGATGAATGGTTGATGTTTCCAAATACAGAAGATGAAGTGGGTGAGATTGTAATTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

203

Amino Acids

22.01

Weight (kDa)

4.79

Isoelectric Point (pI)

56.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016874)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g10310
malus_domestica MD04G1168500.v1.1 MD12G1182100.v1.1
prunus_persica Prupe.6G286800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0461071
rosa_laevigata RLG00000024954
rosa_multiflora Rmu_sc0001521.1_g000061 Rmu_sc0009375.1_g000005
rosa_roxburghii Rroxscaffold_6G00418880
rosa_rugosa Rorug03G0048100
rosa_samantha Rh3AG105500 Rh3BG109500 Rh3CG111100 Rh3DG111200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 102
Acc65I GGTACC 1 cut(s) 181
AccB1I GGYRCC 2 cut(s) 181, 196
AccI GTMKAC 1 cut(s) 275
AciI CCGC 3 cut(s) 72, 179, 242
AcsI RAATTY 1 cut(s) 335
AcuI CTGAAG 4 cut(s) 27, 273, 297, 410
AcvI CACGTG 1 cut(s) 95
AcyI GRCGYC 2 cut(s) 99, 197
AfaI GTAC 1 cut(s) 183
AfiI CCNNNNNNNGG 1 cut(s) 260
AflIII ACRYGT 1 cut(s) 92
AjnI CCWGG 1 cut(s) 259
AluBI AGCT 1 cut(s) 359
AluI AGCT 1 cut(s) 359
AoxI GGCC 1 cut(s) 262
ApeKI GCWGC 3 cut(s) 107, 317, 359
ApoI RAATTY 1 cut(s) 335
Asp718I GGTACC 1 cut(s) 181
AspLEI GCGC 1 cut(s) 199
AspS9I GGNCC 2 cut(s) 221, 263
AsuHPI GGTGA 2 cut(s) 537, 602
AvaII GGWCC 1 cut(s) 221
BanI GGYRCC 2 cut(s) 181, 196
BarI GAAGNNNNNNTAC 2 cut(s) 52, 84
BbrPI CACGTG 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 398
BbvI GCAGC 3 cut(s) 94, 329, 346
BccI CCATC 2 cut(s) 115, 395
BciT130I CCWGG 1 cut(s) 261
BclI TGATCA 2 cut(s) 499, 532
BfaI CTAG 1 cut(s) 380
BfoI RGCGCY 1 cut(s) 200
BisI GCNGC 3 cut(s) 108, 318, 360
BlsI GCNGC 3 cut(s) 109, 319, 361
Bme1390I CCNGG 1 cut(s) 261
Bme18I GGWCC 1 cut(s) 221
BmgT120I GGNCC 2 cut(s) 221, 263
BmiI GGNNCC 5 cut(s) 85, 183, 198, 222, 246
BmrFI CCNGG 1 cut(s) 261
BmrI ACTGGG 1 cut(s) 260
BmsI GCATC 2 cut(s) 94, 514
BmuI ACTGGG 1 cut(s) 260
BpiI GAAGAC 1 cut(s) 398
BsaAI YACGTR 1 cut(s) 95
BsaBI GATNNNNATC 2 cut(s) 157, 498
BsaHI GRCGYC 2 cut(s) 99, 197
BsaJI CCNNGG 2 cut(s) 321, 330
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse1I ACTGG 2 cut(s) 53, 266
Bse8I GATNNNNATC 2 cut(s) 157, 498
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 2 cut(s) 321, 330
BseGI GGATG 1 cut(s) 126
BseJI GATNNNNATC 2 cut(s) 157, 498
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMII CTCAG 1 cut(s) 39
BseNI ACTGG 2 cut(s) 53, 266
BseXI GCAGC 3 cut(s) 94, 329, 346
BshFI GGCC 1 cut(s) 264
BshNI GGYRCC 2 cut(s) 181, 196
BslI CCNNNNNNNGG 1 cut(s) 260
BsnI GGCC 1 cut(s) 264
Bsp143I GATC 3 cut(s) 152, 499, 532
Bsp19I CCATGG 1 cut(s) 321
BspACI CCGC 3 cut(s) 72, 179, 242
BspANI GGCC 1 cut(s) 264
BspCNI CTCAG 1 cut(s) 38
BspLI GGNNCC 5 cut(s) 85, 183, 198, 222, 246
BspT107I GGYRCC 2 cut(s) 181, 196
BsrI ACTGG 2 cut(s) 53, 266
BssECI CCNNGG 2 cut(s) 321, 330
BssMI GATC 3 cut(s) 152, 499, 532
BssNI GRCGYC 2 cut(s) 99, 197
BssT1I CCWWGG 2 cut(s) 321, 330
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 3 cut(s) 228, 485, 511
BstACI GRCGYC 2 cut(s) 99, 197
BstBAI YACGTR 1 cut(s) 95
BstDEI CTNAG 1 cut(s) 25
BstDSI CCRYGG 1 cut(s) 321
BstF5I GGATG 1 cut(s) 126
BstH2I RGCGCY 1 cut(s) 200
BstHHI GCGC 1 cut(s) 199
BstKTI GATC 3 cut(s) 155, 502, 535
BstMBI GATC 3 cut(s) 152, 499, 532
BstMWI GCNNNNNNNGC 1 cut(s) 326
BstNI CCWGG 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 259
BstV1I GCAGC 3 cut(s) 94, 329, 346
BstV2I GAAGAC 1 cut(s) 398
BstXI CCANNNNNNTGG 1 cut(s) 555
BsuRI GGCC 1 cut(s) 264
BtgI CCRYGG 1 cut(s) 321
BtsCI GGATG 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 273
CfoI GCGC 1 cut(s) 199
Cfr13I GGNCC 2 cut(s) 221, 263
CseI GACGC 1 cut(s) 180
Csp6I GTAC 1 cut(s) 182
CviAII CATG 2 cut(s) 134, 322
CviJI RGCY 9 cut(s) 52, 247, 264, 320, 329, 354, 359, 379, 540
CviKI_1 RGCY 9 cut(s) 52, 247, 264, 320, 329, 354, 359, 379, 540
CviQI GTAC 1 cut(s) 182
DdeI CTNAG 1 cut(s) 25
DinI GGCGCC 1 cut(s) 198
DpnI GATC 3 cut(s) 154, 501, 534
DpnII GATC 3 cut(s) 152, 499, 532
EciI GGCGGA 1 cut(s) 257
Eco130I CCWWGG 2 cut(s) 321, 330
Eco47I GGWCC 1 cut(s) 221
Eco57I CTGAAG 4 cut(s) 27, 273, 297, 410
Eco72I CACGTG 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 259
EcoT14I CCWWGG 2 cut(s) 321, 330
EgeI GGCGCC 1 cut(s) 198
EheI GGCGCC 1 cut(s) 198
ErhI CCWWGG 2 cut(s) 321, 330
FaeI CATG 2 cut(s) 137, 325
FaiI YATR 9 cut(s) 135, 140, 150, 214, 216, 251, 303, 323, 367
FatI CATG 2 cut(s) 133, 321
FbaI TGATCA 2 cut(s) 499, 532
FblI GTMKAC 1 cut(s) 275
Fnu4HI GCNGC 3 cut(s) 108, 318, 360
FokI GGATG 1 cut(s) 133
Fsp4HI GCNGC 3 cut(s) 108, 318, 360
FspBI CTAG 1 cut(s) 380
GlaI GCGC 1 cut(s) 198
GluI GCNGC 3 cut(s) 108, 318, 360
HaeII RGCGCY 1 cut(s) 200
HaeIII GGCC 1 cut(s) 264
HgaI GACGC 1 cut(s) 180
HhaI GCGC 1 cut(s) 199
Hin1I GRCGYC 2 cut(s) 99, 197
Hin1II CATG 2 cut(s) 137, 325
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HinfI GANTC 2 cut(s) 127, 233
HphI GGTGA 2 cut(s) 537, 602
Hpy166II GTNNAC 3 cut(s) 69, 224, 276
Hpy188I TCNGA 4 cut(s) 28, 390, 468, 537
Hpy188III TCNNGA 2 cut(s) 118, 503
Hpy8I GTNNAC 3 cut(s) 69, 224, 276
Hpy99I CGWCG 2 cut(s) 104, 196
HpyAV CCTTC 3 cut(s) 57, 297, 318
HpyCH4III ACNGT 3 cut(s) 228, 485, 511
HpyCH4IV ACGT 2 cut(s) 94, 99
HpyCH4V TGCA 2 cut(s) 36, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 326
HpyF3I CTNAG 1 cut(s) 25
HpySE526I ACGT 2 cut(s) 94, 99
Hsp92I GRCGYC 2 cut(s) 99, 197
Hsp92II CATG 2 cut(s) 137, 325
HspAI GCGC 1 cut(s) 197
KasI GGCGCC 1 cut(s) 196
KpnI GGTACC 1 cut(s) 185
Ksp22I TGATCA 2 cut(s) 499, 532
Kzo9I GATC 3 cut(s) 152, 499, 532
LmnI GCTCC 2 cut(s) 244, 310
Lsp1109I GCAGC 3 cut(s) 94, 329, 346
LweI GCATC 2 cut(s) 94, 514
MaeI CTAG 1 cut(s) 380
MaeII ACGT 2 cut(s) 94, 99
MaeIII GTNAC 2 cut(s) 95, 479
MalI GATC 3 cut(s) 154, 501, 534
MboI GATC 3 cut(s) 152, 499, 532
MboII GAAGA 5 cut(s) 33, 72, 303, 403, 590
MluCI AATT 4 cut(s) 37, 335, 451, 600
Mly113I GGCGCC 1 cut(s) 197
MnlI CCTC 3 cut(s) 34, 74, 264
MspA1I CMGCKG 1 cut(s) 179
MspR9I CCNGG 1 cut(s) 261
MvaI CCWGG 1 cut(s) 261
MwoI GCNNNNNNNGC 1 cut(s) 326
NarI GGCGCC 1 cut(s) 197
NcoI CCATGG 1 cut(s) 321
NdeII GATC 3 cut(s) 152, 499, 532
NlaIII CATG 2 cut(s) 137, 325
NlaIV GGNNCC 5 cut(s) 85, 183, 198, 222, 246
NmuCI GTSAC 1 cut(s) 95
PfeI GAWTC 2 cut(s) 127, 233
PkrI GCNGC 3 cut(s) 109, 319, 361
PluTI GGCGCC 1 cut(s) 200
PmaCI CACGTG 1 cut(s) 95
PmlI CACGTG 1 cut(s) 95
Ppu21I YACGTR 1 cut(s) 95
Psp6I CCWGG 1 cut(s) 259
PspCI CACGTG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 259
PspN4I GGNNCC 5 cut(s) 85, 183, 198, 222, 246
PspPI GGNCC 2 cut(s) 221, 263
RsaI GTAC 1 cut(s) 183
RsaNI GTAC 1 cut(s) 182
SatI GCNGC 3 cut(s) 108, 318, 360
Sau3AI GATC 3 cut(s) 152, 499, 532
Sau96I GGNCC 2 cut(s) 221, 263
ScrFI CCNGG 1 cut(s) 261
SetI ASST 7 cut(s) 68, 85, 97, 102, 211, 289, 361
SfaNI GCATC 2 cut(s) 94, 514
SfoI GGCGCC 1 cut(s) 198
SinI GGWCC 1 cut(s) 221
Sse9I AATT 4 cut(s) 37, 335, 451, 600
SsiI CCGC 3 cut(s) 72, 179, 242
SspDI GGCGCC 1 cut(s) 196
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 1 cut(s) 259
StyI CCWWGG 2 cut(s) 321, 330
TaaI ACNGT 3 cut(s) 228, 485, 511
TaiI ACGT 2 cut(s) 97, 102
TaqI TCGA 1 cut(s) 102
TasI AATT 4 cut(s) 37, 335, 451, 600
TfiI GAWTC 2 cut(s) 127, 233
TscAI CASTG 1 cut(s) 273
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 3 cut(s) 107, 317, 359
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 4 cut(s) 225, 354, 567, 597
TspRI CASTG 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 221
XapI RAATTY 1 cut(s) 335
XcmI CCANNNNNNNNNTGG 1 cut(s) 328
XmiI GTMKAC 1 cut(s) 275
XspI CTAG 1 cut(s) 380
ZraI GACGTC 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.