MD12G1182100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016874
Physical Location & Seq
Forward (+)
26185768 .. 26186340
573 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1182100.v1.1.491

Sequence Viewer

Length: 573 bp
ATGAGTTCATCCTCAGAGAAATGCAATTCTTCAGGGAAGCCAACAAGAAGAAGGTTTACAACTAAAAGAGTTTCCAAAAAATGTGACGTCGATGCTGCTGAAATCAGGAGGCAGATTTTCCATGCCATACAACTCCATAGATCATCATCATCAGCTGCACCTGCAGCGGCGGCGCCACCACCACCTGTTTCTATGGGTTCACAGTTTAATTCATTGGTGGAATCTATGCCTCTGCCTGGGCCGGTGTGGTCAACAACTGCACCTTCAGTATCGCCTCATGCTCCTTCAGCAGCCATGGAAGCCTTGGAATTTGAGTGGGGGGAGAGCCAAGCTGCGTCATATTCTTGGTGGCTAGGGTTTCTGAAGACTTTGGATGGCAAGAATGCTGACAAGTCAAACTACCCTTTGCCATTGGGGAGTCCTAATTGGTTGTTTGGTCAGAACCAGAACAGTAACTGTAAAGTAGGTGATCATCAAGAGACAGCTGCTTTGGTTGATGCAAATGATCAGAGCCCTTCACCAGATGAATGGTTGATGTTTCCAGAAGATGAGGGTGAGACTGTAATTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

20.59

Weight (kDa)

5.35

Isoelectric Point (pI)

66.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016874)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g10310
malus_domestica MD04G1168500.v1.1 MD12G1182100.v1.1
prunus_persica Prupe.6G286800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0461071
rosa_laevigata RLG00000024954
rosa_multiflora Rmu_sc0001521.1_g000061 Rmu_sc0009375.1_g000005
rosa_roxburghii Rroxscaffold_6G00418880
rosa_rugosa Rorug03G0048100
rosa_samantha Rh3AG105500 Rh3BG109500 Rh3CG111100 Rh3DG111200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 169
AatII GACGTC 1 cut(s) 90
Acc36I ACCTGC 1 cut(s) 169
AccB1I GGYRCC 1 cut(s) 172
AciI CCGC 2 cut(s) 167, 170
AcsI RAATTY 1 cut(s) 308
AcuI CTGAAG 4 cut(s) 15, 249, 270, 383
AcyI GRCGYC 2 cut(s) 87, 173
AfiI CCNNNNNNNGG 1 cut(s) 236
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 3 cut(s) 155, 332, 485
AluI AGCT 3 cut(s) 155, 332, 485
Alw26I GTCTC 2 cut(s) 473, 551
AlwNI CAGNNNCTG 1 cut(s) 456
AoxI GGCC 1 cut(s) 239
ApeKI GCWGC 6 cut(s) 95, 155, 164, 290, 332, 485
ApoI RAATTY 1 cut(s) 308
ArsI GACNNNNNNTTYG 2 cut(s) 472, 504
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 3 cut(s) 479, 510, 566
BanI GGYRCC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 515
BarI GAAGNNNNNNTAC 2 cut(s) 40, 72
BbsI GAAGAC 1 cut(s) 371
BbvI GCAGC 6 cut(s) 82, 142, 176, 302, 319, 472
BccI CCATC 1 cut(s) 368
BciT130I CCWGG 1 cut(s) 237
BclI TGATCA 2 cut(s) 469, 505
BcoDI GTCTC 2 cut(s) 473, 551
BfaI CTAG 1 cut(s) 353
BfmI CTRYAG 1 cut(s) 162
BfoI RGCGCY 1 cut(s) 176
BfuAI ACCTGC 1 cut(s) 169
BisI GCNGC 8 cut(s) 96, 156, 165, 168, 171, 291, 333, 486
BlsI GCNGC 8 cut(s) 97, 157, 166, 169, 172, 292, 334, 487
Bme1390I CCNGG 1 cut(s) 237
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 237
BmsI GCATC 2 cut(s) 82, 487
BpiI GAAGAC 1 cut(s) 371
BsaBI GATNNNNATC 1 cut(s) 145
BsaHI GRCGYC 2 cut(s) 87, 173
BsaJI CCNNGG 3 cut(s) 236, 294, 303
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse118I RCCGGY 1 cut(s) 241
Bse8I GATNNNNATC 1 cut(s) 145
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 3 cut(s) 236, 294, 303
BseGI GGATG 2 cut(s) 8, 379
BseJI GATNNNNATC 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMII CTCAG 1 cut(s) 27
BseXI GCAGC 6 cut(s) 82, 142, 176, 302, 319, 472
BsgI GTGCAG 2 cut(s) 141, 243
BshFI GGCC 1 cut(s) 241
BshNI GGYRCC 1 cut(s) 172
BsiSI CCGG 1 cut(s) 242
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 2 cut(s) 473, 551
BsmI GAATGC 1 cut(s) 388
BsnI GGCC 1 cut(s) 241
Bsp1286I GDGCHC 1 cut(s) 515
Bsp143I GATC 3 cut(s) 140, 469, 505
Bsp19I CCATGG 1 cut(s) 294
BspACI CCGC 2 cut(s) 167, 170
BspANI GGCC 1 cut(s) 241
BspCNI CTCAG 1 cut(s) 26
BspLI GGNNCC 1 cut(s) 174
BspMAI CTGCAG 1 cut(s) 166
BspMI ACCTGC 1 cut(s) 169
BspT107I GGYRCC 1 cut(s) 172
BsrFI RCCGGY 1 cut(s) 241
BssAI RCCGGY 1 cut(s) 241
BssECI CCNNGG 3 cut(s) 236, 294, 303
BssMI GATC 3 cut(s) 140, 469, 505
BssNI GRCGYC 2 cut(s) 87, 173
BssT1I CCWWGG 2 cut(s) 294, 303
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 4 cut(s) 204, 452, 458, 562
BstACI GRCGYC 2 cut(s) 87, 173
BstDEI CTNAG 1 cut(s) 13
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 2 cut(s) 8, 379
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 3 cut(s) 143, 472, 508
BstMAI GTCTC 2 cut(s) 473, 551
BstMBI GATC 3 cut(s) 140, 469, 505
BstMWI GCNNNNNNNGC 5 cut(s) 161, 164, 170, 287, 299
BstNI CCWGG 1 cut(s) 237
BstSCI CCNGG 1 cut(s) 235
BstSFI CTRYAG 1 cut(s) 162
BstV1I GCAGC 6 cut(s) 82, 142, 176, 302, 319, 472
BstV2I GAAGAC 1 cut(s) 371
BstXI CCANNNNNNTGG 1 cut(s) 528
BsuRI GGCC 1 cut(s) 241
BtgI CCRYGG 1 cut(s) 294
BtsCI GGATG 2 cut(s) 8, 379
BveI ACCTGC 1 cut(s) 169
CaiI CAGNNNCTG 1 cut(s) 456
CfoI GCGC 1 cut(s) 175
Cfr10I RCCGGY 1 cut(s) 241
Cfr13I GGNCC 1 cut(s) 239
CseI GACGC 1 cut(s) 324
CviAII CATG 3 cut(s) 122, 278, 295
DdeI CTNAG 1 cut(s) 13
DinI GGCGCC 1 cut(s) 174
DpnI GATC 3 cut(s) 142, 471, 507
DpnII GATC 3 cut(s) 140, 469, 505
Eco130I CCWWGG 2 cut(s) 294, 303
Eco24I GRGCYC 1 cut(s) 515
Eco57I CTGAAG 4 cut(s) 15, 249, 270, 383
EcoRII CCWGG 1 cut(s) 235
EcoT14I CCWWGG 2 cut(s) 294, 303
EcoT38I GRGCYC 1 cut(s) 515
EgeI GGCGCC 1 cut(s) 174
EheI GGCGCC 1 cut(s) 174
ErhI CCWWGG 2 cut(s) 294, 303
FaeI CATG 3 cut(s) 125, 281, 298
FaiI YATR 8 cut(s) 123, 128, 138, 194, 227, 279, 296, 340
FatI CATG 3 cut(s) 121, 277, 294
FbaI TGATCA 2 cut(s) 469, 505
Fnu4HI GCNGC 8 cut(s) 96, 156, 165, 168, 171, 291, 333, 486
FokI GGATG 1 cut(s) 386
FriOI GRGCYC 1 cut(s) 515
Fsp4HI GCNGC 8 cut(s) 96, 156, 165, 168, 171, 291, 333, 486
FspBI CTAG 1 cut(s) 353
GlaI GCGC 1 cut(s) 174
GluI GCNGC 8 cut(s) 96, 156, 165, 168, 171, 291, 333, 486
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 1 cut(s) 241
HapII CCGG 1 cut(s) 242
HgaI GACGC 1 cut(s) 324
HhaI GCGC 1 cut(s) 175
Hin1I GRCGYC 2 cut(s) 87, 173
Hin1II CATG 3 cut(s) 125, 281, 298
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HinfI GANTC 2 cut(s) 221, 418
HpaII CCGG 1 cut(s) 242
HphI GGTGA 3 cut(s) 479, 510, 566
Hpy166II GTNNAC 3 cut(s) 57, 200, 252
Hpy188I TCNGA 4 cut(s) 16, 363, 441, 510
Hpy188III TCNNGA 3 cut(s) 106, 476, 542
Hpy8I GTNNAC 3 cut(s) 57, 200, 252
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 4 cut(s) 45, 273, 294, 525
HpyCH4III ACNGT 4 cut(s) 204, 452, 458, 562
HpyCH4IV ACGT 1 cut(s) 87
HpyCH4V TGCA 5 cut(s) 24, 158, 164, 260, 500
HpyF10VI GCNNNNNNNGC 5 cut(s) 161, 164, 170, 287, 299
HpyF3I CTNAG 1 cut(s) 13
HpySE526I ACGT 1 cut(s) 87
Hsp92I GRCGYC 2 cut(s) 87, 173
Hsp92II CATG 3 cut(s) 125, 281, 298
HspAI GCGC 1 cut(s) 173
KasI GGCGCC 1 cut(s) 172
Ksp22I TGATCA 2 cut(s) 469, 505
Kzo9I GATC 3 cut(s) 140, 469, 505
LmnI GCTCC 1 cut(s) 286
Lsp1109I GCAGC 6 cut(s) 82, 142, 176, 302, 319, 472
LweI GCATC 2 cut(s) 82, 487
MaeI CTAG 1 cut(s) 353
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 2 cut(s) 83, 452
MalI GATC 3 cut(s) 142, 471, 507
MboI GATC 3 cut(s) 140, 469, 505
MboII GAAGA 4 cut(s) 21, 60, 376, 557
MhlI GDGCHC 1 cut(s) 515
MluCI AATT 5 cut(s) 25, 208, 308, 424, 564
Mly113I GGCGCC 1 cut(s) 173
MlyI GAGTC 1 cut(s) 427
MnlI CCTC 5 cut(s) 22, 102, 240, 285, 544
MseI TTAA 2 cut(s) 207, 571
MspA1I CMGCKG 3 cut(s) 155, 167, 485
MspI CCGG 1 cut(s) 242
MspR9I CCNGG 1 cut(s) 237
Mva1269I GAATGC 1 cut(s) 388
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 5 cut(s) 161, 164, 170, 287, 299
NarI GGCGCC 1 cut(s) 173
NcoI CCATGG 1 cut(s) 294
NdeII GATC 3 cut(s) 140, 469, 505
NlaIII CATG 3 cut(s) 125, 281, 298
NlaIV GGNNCC 1 cut(s) 174
NmuCI GTSAC 1 cut(s) 83
PaqCI CACCTGC 1 cut(s) 169
PctI GAATGC 1 cut(s) 388
PfeI GAWTC 1 cut(s) 221
PkrI GCNGC 8 cut(s) 97, 157, 166, 169, 172, 292, 334, 487
PleI GAGTC 1 cut(s) 426
PluTI GGCGCC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 426
Psp6I CCWGG 1 cut(s) 235
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 1 cut(s) 239
PstI CTGCAG 1 cut(s) 166
PstNI CAGNNNCTG 1 cut(s) 456
PvuII CAGCTG 2 cut(s) 155, 485
SaqAI TTAA 2 cut(s) 207, 571
SatI GCNGC 8 cut(s) 96, 156, 165, 168, 171, 291, 333, 486
Sau3AI GATC 3 cut(s) 140, 469, 505
Sau96I GGNCC 1 cut(s) 239
SchI GAGTC 1 cut(s) 427
ScrFI CCNGG 1 cut(s) 237
SduI GDGCHC 1 cut(s) 515
SetI ASST 9 cut(s) 56, 90, 157, 163, 187, 265, 334, 469, 487
SfaNI GCATC 2 cut(s) 82, 487
SfcI CTRYAG 1 cut(s) 162
SfoI GGCGCC 1 cut(s) 174
Sse9I AATT 5 cut(s) 25, 208, 308, 424, 564
SsiI CCGC 2 cut(s) 167, 170
SspDI GGCGCC 1 cut(s) 172
SspMI CTAG 1 cut(s) 353
StyD4I CCNGG 1 cut(s) 235
StyI CCWWGG 2 cut(s) 294, 303
TaaI ACNGT 4 cut(s) 204, 452, 458, 562
TaiI ACGT 1 cut(s) 90
TaqI TCGA 1 cut(s) 90
TasI AATT 5 cut(s) 25, 208, 308, 424, 564
TauI GCSGC 2 cut(s) 170, 173
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 2 cut(s) 207, 571
Tru9I TTAA 2 cut(s) 207, 571
TseFI GTSAC 1 cut(s) 83
TseI GCWGC 6 cut(s) 95, 155, 164, 290, 332, 485
Tsp45I GTSAC 1 cut(s) 83
TspDTI ATGAA 2 cut(s) 201, 540
XapI RAATTY 1 cut(s) 308
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
XspI CTAG 1 cut(s) 353
ZraI GACGTC 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.