MD04G1172900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013517
Physical Location & Seq
Forward (+)
26437110 .. 26439795
2686 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1172900.v1.1.491

Sequence Viewer

Length: 681 bp
ATGGCGGGAAGTGGAAGGGGGTCGAGATTTGCACTCCTGCTGCTCCCTCTGTTGATTTCTTTCTCCATGCTGCCCTGCATATCTGCCGCTTACAAGCCAGGCGACATCGTGCCCATGAGCAAGATGGGCCTTTATCACTCTATGAAAACCGAATGGCACGATATGATCGGTCGACACTGCCCAATTTTTGCCGTGAATCGTGAGGTGTTAGTTCCTCTAGTGAAGCCGATGGGCTATACAGGAGCTGATGCTTATAAAATATCATTTCAAATTGGGAGAGAAAAAATTTTAGTACCTTGGCTTTTTGTGATAAATCGTAAAAGTTCCGAGGTCCCAATGATTGATGTCCATTTGAGGTATTCAGGAAGTGATTTGCATGGTGTCACTGCTAAAGTTGTGGATATGCCTCACCACTATGTAGAAATCCATCCGGATGTTCGTAAACAATTTTGGGATGCTCAGCGCTGGCCAAAGCATGTGTTGGTCAGATATACATGGGAGGAGCAATCAGAGATAGACGTGACATCTGGATTTTATGTACTGTTTGGATCGGGTCTCATGCTTTCTTTTATTCTGTCGATATACATTTTGCAATCATCACGGGACAAGTTAGCAAGGTTTGTGAGGGAGAGAGTCGCAGAAAGCAACATGCCTGCTGGAGGTGTGGCAAAGGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

25.65

Weight (kDa)

9.18

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013517)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51610
fragaria_vesca FvH4_6g10100
malus_domestica MD04G1172900.v1.1 MD11G1271600.v1.1 MD12G1088300.v1.1 MD12G1186200.v1.1
prunus_persica Prupe.6G291700_v2.0.a1
pyrus_communis pycom04g15280
rosa_chinensis RchiOBHm_Chr3g0460801
rosa_laevigata RLG00000024980
rosa_multiflora Rmu_sc0007678.1_g000016
rosa_roxburghii Rroxscaffold_6G00419210
rosa_rugosa Rorug03G0046500
rosa_samantha Rh3AG103500 Rh3BG107200 Rh3CG109100 Rh3DG109000
rosa_wichuraiana Rw3G008810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 255
AccI GTMKAC 1 cut(s) 172
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 2 cut(s) 5, 87
AclWI GGATC 1 cut(s) 556
AcoI YGGCCR 1 cut(s) 467
AcsI RAATTY 1 cut(s) 285
AfaI GTAC 2 cut(s) 294, 540
AfeI AGCGCT 1 cut(s) 464
AfiI CCNNNNNNNGG 1 cut(s) 659
AgsI TTSAA 2 cut(s) 269, 677
AjiI CACGTC 1 cut(s) 520
AjnI CCWGG 1 cut(s) 97
AluBI AGCT 1 cut(s) 245
AluI AGCT 1 cut(s) 245
Alw26I GTCTC 1 cut(s) 560
AlwI GGATC 1 cut(s) 556
AlwNI CAGNNNCTG 1 cut(s) 245
Aor13HI TCCGGA 1 cut(s) 430
Aor51HI AGCGCT 1 cut(s) 464
AoxI GGCC 2 cut(s) 127, 467
ApeKI GCWGC 2 cut(s) 40, 70
ApoI RAATTY 1 cut(s) 285
AspLEI GCGC 1 cut(s) 465
AspS9I GGNCC 2 cut(s) 127, 331
AsuHPI GGTGA 1 cut(s) 401
AvaII GGWCC 1 cut(s) 331
BaeGI GKGCMC 1 cut(s) 114
BalI TGGCCA 1 cut(s) 469
BbvI GCAGC 2 cut(s) 27, 57
BccI CCATC 3 cut(s) 118, 223, 435
BceAI ACGGC 1 cut(s) 176
BciT130I CCWGG 1 cut(s) 99
BcoDI GTCTC 1 cut(s) 560
BfaI CTAG 1 cut(s) 218
BfoI RGCGCY 1 cut(s) 466
BisI GCNGC 3 cut(s) 41, 71, 87
BlpI GCTNAGC 1 cut(s) 459
BlsI GCNGC 3 cut(s) 42, 72, 88
Bme1390I CCNGG 1 cut(s) 99
Bme18I GGWCC 1 cut(s) 331
BmgBI CACGTC 1 cut(s) 520
BmgT120I GGNCC 2 cut(s) 127, 331
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 1 cut(s) 99
BmsI GCATC 2 cut(s) 238, 445
BpmI CTGGAG 1 cut(s) 678
Bpu1102I GCTNAGC 1 cut(s) 459
BsaI GGTCTC 1 cut(s) 560
BsaJI CCNNGG 2 cut(s) 296, 327
BsaWI WCCGGW 1 cut(s) 430
Bsc4I CCNNNNNNNGG 1 cut(s) 659
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 1 cut(s) 99
BseDI CCNNGG 2 cut(s) 296, 327
BseGI GGATG 3 cut(s) 427, 439, 460
BseLI CCNNNNNNNGG 1 cut(s) 659
BseMII CTCAG 1 cut(s) 473
BseRI GAGGAG 1 cut(s) 515
BseSI GKGCMC 1 cut(s) 114
BseXI GCAGC 2 cut(s) 27, 57
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 2 cut(s) 129, 469
BsiEI CGRYCG 1 cut(s) 172
BsiSI CCGG 1 cut(s) 431
BslFI GGGAC 2 cut(s) 317, 617
BslI CCNNNNNNNGG 1 cut(s) 659
BsmAI GTCTC 1 cut(s) 560
BsmFI GGGAC 2 cut(s) 317, 617
BsnI GGCC 2 cut(s) 129, 469
Bso31I GGTCTC 1 cut(s) 560
Bsp1286I GDGCHC 1 cut(s) 114
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 2 cut(s) 165, 548
Bsp1720I GCTNAGC 1 cut(s) 459
BspACI CCGC 2 cut(s) 5, 87
BspANI GGCC 2 cut(s) 129, 469
BspCNI CTCAG 1 cut(s) 472
BspEI TCCGGA 1 cut(s) 430
BspLI GGNNCC 1 cut(s) 333
BspPI GGATC 1 cut(s) 556
BspTNI GGTCTC 1 cut(s) 560
BssECI CCNNGG 2 cut(s) 296, 327
BssMI GATC 2 cut(s) 165, 548
BssT1I CCWWGG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 543
BstC8I GCNNGC 2 cut(s) 467, 654
BstDEI CTNAG 1 cut(s) 459
BstENI CCTNNNNNAGG 1 cut(s) 657
BstF5I GGATG 3 cut(s) 427, 439, 460
BstH2I RGCGCY 1 cut(s) 466
BstHHI GCGC 1 cut(s) 465
BstKTI GATC 2 cut(s) 168, 551
BstMAI GTCTC 1 cut(s) 560
BstMBI GATC 2 cut(s) 165, 548
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 1 cut(s) 126
BstNI CCWGG 1 cut(s) 99
BstNSI RCATGY 2 cut(s) 479, 652
BstSCI CCNGG 1 cut(s) 97
BstSLI GKGCMC 1 cut(s) 114
BstV1I GCAGC 2 cut(s) 27, 57
BsuRI GGCC 2 cut(s) 129, 469
BtrI CACGTC 1 cut(s) 520
BtsCI GGATG 3 cut(s) 427, 439, 460
BtsI GCAGTG 2 cut(s) 175, 384
BtsIMutI CAGTG 2 cut(s) 175, 384
Cac8I GCNNGC 2 cut(s) 467, 654
CaiI CAGNNNCTG 1 cut(s) 245
CfoI GCGC 1 cut(s) 465
Cfr13I GGNCC 2 cut(s) 127, 331
Csp6I GTAC 2 cut(s) 293, 539
CviAII CATG 7 cut(s) 67, 115, 377, 476, 495, 559, 649
CviJI RGCY 7 cut(s) 97, 129, 226, 234, 245, 301, 469
CviKI_1 RGCY 7 cut(s) 97, 129, 226, 234, 245, 301, 469
CviQI GTAC 2 cut(s) 293, 539
DdeI CTNAG 1 cut(s) 459
DpnI GATC 2 cut(s) 167, 550
DpnII GATC 2 cut(s) 165, 548
EaeI YGGCCR 1 cut(s) 467
Eco130I CCWWGG 1 cut(s) 296
Eco31I GGTCTC 1 cut(s) 560
Eco47I GGWCC 1 cut(s) 331
Eco47III AGCGCT 1 cut(s) 464
EcoNI CCTNNNNNAGG 1 cut(s) 657
EcoO109I RGGNCCY 1 cut(s) 331
EcoRII CCWGG 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 7 cut(s) 70, 118, 380, 479, 498, 562, 652
FaqI GGGAC 2 cut(s) 317, 617
FatI CATG 7 cut(s) 66, 114, 376, 475, 494, 558, 648
FblI GTMKAC 1 cut(s) 172
Fnu4HI GCNGC 3 cut(s) 41, 71, 87
FokI GGATG 3 cut(s) 414, 446, 467
Fsp4HI GCNGC 3 cut(s) 41, 71, 87
FspBI CTAG 1 cut(s) 218
GlaI GCGC 1 cut(s) 464
GluI GCNGC 3 cut(s) 41, 71, 87
GsuI CTGGAG 1 cut(s) 678
HaeII RGCGCY 1 cut(s) 466
HaeIII GGCC 2 cut(s) 129, 469
HapII CCGG 1 cut(s) 431
HhaI GCGC 1 cut(s) 465
Hin1II CATG 7 cut(s) 70, 118, 380, 479, 498, 562, 652
Hin6I GCGC 1 cut(s) 463
HinP1I GCGC 1 cut(s) 463
HincII GTYRAC 1 cut(s) 173
HindII GTYRAC 1 cut(s) 173
HinfI GANTC 2 cut(s) 196, 633
HpaII CCGG 1 cut(s) 431
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 2 cut(s) 173, 443
Hpy188I TCNGA 3 cut(s) 328, 488, 511
Hpy188III TCNNGA 5 cut(s) 24, 200, 363, 431, 528
Hpy8I GTNNAC 2 cut(s) 173, 443
HpyAV CCTTC 1 cut(s) 9
HpyCH4III ACNGT 1 cut(s) 543
HpyCH4IV ACGT 1 cut(s) 519
HpyCH4V TGCA 4 cut(s) 32, 78, 376, 592
HpyF10VI GCNNNNNNNGC 1 cut(s) 126
HpyF3I CTNAG 1 cut(s) 459
HpySE526I ACGT 1 cut(s) 519
Hsp92II CATG 7 cut(s) 70, 118, 380, 479, 498, 562, 652
HspAI GCGC 1 cut(s) 463
Kpn2I TCCGGA 1 cut(s) 430
Kzo9I GATC 2 cut(s) 165, 548
LmnI GCTCC 3 cut(s) 48, 242, 502
Lsp1109I GCAGC 2 cut(s) 27, 57
LweI GCATC 2 cut(s) 238, 445
MaeI CTAG 1 cut(s) 218
MaeII ACGT 1 cut(s) 519
MaeIII GTNAC 2 cut(s) 382, 520
MalI GATC 2 cut(s) 167, 550
MboI GATC 2 cut(s) 165, 548
MhlI GDGCHC 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 469
MluCI AATT 4 cut(s) 183, 270, 285, 446
MluNI TGGCCA 1 cut(s) 469
MlyI GAGTC 1 cut(s) 642
MnlI CCTC 9 cut(s) 57, 196, 225, 322, 348, 417, 493, 618, 653
Mox20I TGGCCA 1 cut(s) 469
MroI TCCGGA 1 cut(s) 430
MscI TGGCCA 1 cut(s) 469
MslI CAYNNNNRTG 2 cut(s) 414, 432
Msp20I TGGCCA 1 cut(s) 469
MspI CCGG 1 cut(s) 431
MspR9I CCNGG 1 cut(s) 99
MvaI CCWGG 1 cut(s) 99
MwoI GCNNNNNNNGC 1 cut(s) 126
NdeII GATC 2 cut(s) 165, 548
NlaIII CATG 7 cut(s) 70, 118, 380, 479, 498, 562, 652
NlaIV GGNNCC 1 cut(s) 333
NmuCI GTSAC 2 cut(s) 382, 520
NspI RCATGY 2 cut(s) 479, 652
PfeI GAWTC 1 cut(s) 196
PkrI GCNGC 3 cut(s) 42, 72, 88
PleI GAGTC 1 cut(s) 641
PpsI GAGTC 1 cut(s) 641
PpuMI RGGWCCY 1 cut(s) 331
PsiI TTATAA 1 cut(s) 255
Psp5II RGGWCCY 1 cut(s) 331
Psp6I CCWGG 1 cut(s) 97
PspGI CCWGG 1 cut(s) 97
PspN4I GGNNCC 1 cut(s) 333
PspPI GGNCC 2 cut(s) 127, 331
PspPPI RGGWCCY 1 cut(s) 331
PstNI CAGNNNCTG 1 cut(s) 245
RsaI GTAC 2 cut(s) 294, 540
RsaNI GTAC 2 cut(s) 293, 539
RseI CAYNNNNRTG 2 cut(s) 414, 432
SalI GTCGAC 1 cut(s) 171
SatI GCNGC 3 cut(s) 41, 71, 87
Sau3AI GATC 2 cut(s) 165, 548
Sau96I GGNCC 2 cut(s) 127, 331
SchI GAGTC 1 cut(s) 642
ScrFI CCNGG 1 cut(s) 99
SduI GDGCHC 1 cut(s) 114
SetI ASST 9 cut(s) 207, 247, 298, 333, 359, 522, 620, 664, 675
SfaNI GCATC 2 cut(s) 238, 445
SinI GGWCC 1 cut(s) 331
SmiMI CAYNNNNRTG 2 cut(s) 414, 432
Sse9I AATT 4 cut(s) 183, 270, 285, 446
SsiI CCGC 2 cut(s) 5, 87
SspMI CTAG 1 cut(s) 218
StyD4I CCNGG 1 cut(s) 97
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 1 cut(s) 543
TaiI ACGT 1 cut(s) 522
TaqI TCGA 3 cut(s) 23, 172, 578
TaqII GACCGA 1 cut(s) 158
TasI AATT 4 cut(s) 183, 270, 285, 446
TatI WGTACW 1 cut(s) 538
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 1 cut(s) 196
TscAI CASTG 2 cut(s) 182, 391
TseFI GTSAC 2 cut(s) 382, 520
TseI GCWGC 2 cut(s) 40, 70
Tsp45I GTSAC 2 cut(s) 382, 520
TspDTI ATGAA 1 cut(s) 158
TspRI CASTG 2 cut(s) 182, 391
VpaK11BI GGWCC 1 cut(s) 331
XagI CCTNNNNNAGG 1 cut(s) 657
XapI RAATTY 1 cut(s) 285
XceI RCATGY 2 cut(s) 479, 652
XcmI CCANNNNNNNNNTGG 1 cut(s) 121
XmiI GTMKAC 1 cut(s) 172
XspI CTAG 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.