Rorug03G0046500

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0008549
Physical Location & Seq
Forward (+)
3579573 .. 3583269
3697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0046500.1

Sequence Viewer

Length: 1539 bp
ATGGCTTGCAACAAAGAGGAGGCAATCAAGGCAATGCAACTTGCTGAAAGAAAGACGCAGGATAATGATTTTACAGGAGCAAGGAAGATAGCACAGAAGGCTCAACAACTCTTCCCGGGGCTTGAGAATATTTCTCAATTACTTGCAGTATGTGAAGTTCATTGTTCAGCTGAAAATAAGCTAGATGGCAGTGAAATGGACTGGTATGGGATTCTGCAGGTTCAAAGATTTTCTCATGAGGCAATCATCAAGAAACAATTCAGAAAACTCGGGCTGTTACTTAATCCTGATAACAACAAGTTTGCTGGTGCGGAGGCTGCTTTTAAGCTGATCGGGGAAGCAAATGCAGTGCTGGACGACCGAGGAAAACATTTTAGATATGACATGAAGTGTAGAGCTCTTCTGAGAACTGGTGTAATACAATCATCATCTCATCAGTTTAATGAAAATGTATTTGTCAGGAAACACTGTGATGCTGCAAGAAATGTTCAGAATATTCCCGAGTCACAGTGTGCTAGCATGAATGAACTTCAAAAGGGGAAAGCAGACACATTCTGCACTTGTTGTCCTCTCTGTAAAACAGTGTATCAATGTGCCAAAGACTCGGCAAATCGATTGTTACGTTGTCAAAAATGTCGAAGACTTTTTGTTGCCTCTGATTGTGGTATTCAAGGGGTGCACCTAGAAACTCTCAGGAACCAGTTTGCAAATCATACAGAACTTCCTAACCAGATTCCTATACAAGTAGCCTCAAATCATGGTGGAACTGGAAATCTACCTGTTACAAGATCTCAAACTGGAAATGCAGCCTCAAATCCTGTGTCAAAGGCGGGAGTTGCTGCTGATGTTGATGGAGCTTCTAACACTCAAAAGAAAAATAGTGAGCATGGGGTTGCAGTAAGGAAGGAGGGTGTTGAAATATCCAAGTCTGGTTCAGTGAAGTCCAAGGAGTCAGGACCTTTGAGAAATCTGAACAAAAAAAGAGGGAAGTCAATTGAATCTGTTGGAAGTTTCAAGACTGGAAACAGAGCTAGACCTGATTCTGAGCATGTTATTCAAGAAAAAGTCGGTATTCCTTCTGAACTCAATGGAAGCCCTCACCTTTGCATATCTTCGATGAAAGAGAAGAACCTTTCTTACATTGGTAATGATGATGATGATGATTTTGTGAACCCTCCAAACTGGTCAGGGAAGAGCCCACTGTCCAGTGCTACTGAAAAGAAAAGAAAGAATGCAGCTGCGTATGGTGGGTGTGGGTCATTTAATATTGATAGGTCACCTTATTCTGCTGATGCTGCTGTGGATGGACATAAGAAGAAGGCAAGACAAAAAGTAAGCTTCCCTTTAGAAGAATGTATGCCCAGCAAAAAGAGTAAAACTGGGGAACCTGTGCCAGATAATGATGAAACCAACTTCAAAGCTAATGTCACTCCTGGGCCAAATGTAGATGTCGCATCCACACCACAACTCATTGAGATGGCATGCCCAGGTACTATGCTTTTGTCAAGAAAGTATTACACACCCCGGGATTTAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

512

Amino Acids

55.97

Weight (kDa)

9.0

Isoelectric Point (pI)

36.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 67 - 128 5.5e-13 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 208
AciI CCGC 2 cut(s) 311, 830
AdeI CACNNNGTG 1 cut(s) 512
AfaI GTAC 1 cut(s) 1492
AgsI TTSAA 8 cut(s) 224, 533, 671, 917, 998, 1015, 1058, 1417
AjnI CCWGG 2 cut(s) 1432, 1486
Alw21I GWGCWC 2 cut(s) 400, 681
Alw44I GTGCAC 1 cut(s) 677
Ama87I CYCGRG 4 cut(s) 115, 269, 500, 1524
AoxI GGCC 1 cut(s) 1436
ApaLI GTGCAC 1 cut(s) 677
ApeKI GCWGC 7 cut(s) 317, 476, 806, 839, 1235, 1238, 1295
ArsI GACNNNNNNTTYG 4 cut(s) 806, 838, 1410, 1442
Asp700I GAANNNNTTC 1 cut(s) 257
AspS9I GGNCC 2 cut(s) 956, 1436
AsuC2I CCSGG 4 cut(s) 116, 117, 1525, 1526
AsuHPI GGTGA 2 cut(s) 1091, 1269
AsuNHI GCTAGC 1 cut(s) 515
AvaI CYCGRG 4 cut(s) 115, 269, 500, 1524
AvaII GGWCC 1 cut(s) 956
BaeGI GKGCMC 1 cut(s) 681
BanII GRGCYC 2 cut(s) 400, 1199
BbsI GAAGAC 1 cut(s) 646
Bbv12I GWGCWC 2 cut(s) 400, 681
BbvI GCAGC 7 cut(s) 304, 463, 818, 826, 1225, 1247, 1282
BccI CCATC 4 cut(s) 179, 845, 1298, 1471
BciT130I CCWGG 2 cut(s) 1434, 1488
BcnI CCSGG 4 cut(s) 116, 117, 1525, 1526
BfaI CTAG 4 cut(s) 182, 516, 683, 1032
BfmI CTRYAG 1 cut(s) 215
BfuAI ACCTGC 1 cut(s) 208
BglII AGATCT 1 cut(s) 788
BisI GCNGC 7 cut(s) 318, 477, 807, 840, 1236, 1239, 1296
BlsI GCNGC 7 cut(s) 319, 478, 808, 841, 1237, 1240, 1297
Bme1390I CCNGG 6 cut(s) 116, 117, 1434, 1488, 1525, 1526
Bme18I GGWCC 1 cut(s) 956
BmeT110I CYCGRG 4 cut(s) 115, 269, 500, 1524
BmgT120I GGNCC 2 cut(s) 956, 1436
BmiI GGNNCC 2 cut(s) 698, 1386
BmrFI CCNGG 6 cut(s) 116, 117, 1434, 1488, 1525, 1526
BmrI ACTGGG 1 cut(s) 1389
BmsI GCATC 3 cut(s) 463, 1282, 1463
BmtI GCTAGC 1 cut(s) 519
BmuI ACTGGG 1 cut(s) 1389
BpiI GAAGAC 1 cut(s) 646
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 4 cut(s) 116, 117, 1525, 1526
Bsa29I ATCGAT 1 cut(s) 613
BsaJI CCNNGG 8 cut(s) 115, 116, 361, 945, 1433, 1486, 1523, 1524
Bse1I ACTGG 9 cut(s) 206, 415, 700, 772, 802, 1024, 1187, 1206, 1384
Bse3DI GCAATG 1 cut(s) 39
BseBI CCWGG 2 cut(s) 1434, 1488
BseCI ATCGAT 1 cut(s) 613
BseDI CCNNGG 8 cut(s) 115, 116, 361, 945, 1433, 1486, 1523, 1524
BseGI GGATG 2 cut(s) 1309, 1454
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 3 cut(s) 395, 706, 1035
BseNI ACTGG 9 cut(s) 206, 415, 700, 772, 802, 1024, 1187, 1206, 1384
BseRI GAGGAG 1 cut(s) 32
BseSI GKGCMC 1 cut(s) 681
BseXI GCAGC 7 cut(s) 304, 463, 818, 826, 1225, 1247, 1282
BseYI CCCAGC 1 cut(s) 1361
BsgI GTGCAG 1 cut(s) 541
Bsh1285I CGRYCG 1 cut(s) 361
BshFI GGCC 1 cut(s) 1438
BshVI ATCGAT 1 cut(s) 613
BsiEI CGRYCG 1 cut(s) 361
BsiHKAI GWGCWC 2 cut(s) 400, 681
BsiHKCI CYCGRG 4 cut(s) 115, 269, 500, 1524
BsiSI CCGG 2 cut(s) 116, 1525
BsmI GAATGC 1 cut(s) 1237
BsnI GGCC 1 cut(s) 1438
BsoBI CYCGRG 4 cut(s) 115, 269, 500, 1524
Bsp1286I GDGCHC 3 cut(s) 400, 681, 1199
Bsp143I GATC 2 cut(s) 330, 788
BspACI CCGC 2 cut(s) 311, 830
BspANI GGCC 1 cut(s) 1438
BspCNI CTCAG 3 cut(s) 396, 705, 1036
BspDI ATCGAT 1 cut(s) 613
BspHI TCATGA 1 cut(s) 235
BspLI GGNNCC 2 cut(s) 698, 1386
BspMAI CTGCAG 1 cut(s) 219
BspMI ACCTGC 1 cut(s) 208
BspOI GCTAGC 1 cut(s) 519
BspQI GCTCTTC 2 cut(s) 405, 1187
BsrDI GCAATG 1 cut(s) 39
BsrI ACTGG 9 cut(s) 206, 415, 700, 772, 802, 1024, 1187, 1206, 1384
BssECI CCNNGG 8 cut(s) 115, 116, 361, 945, 1433, 1486, 1523, 1524
BssMI GATC 2 cut(s) 330, 788
BssT1I CCWWGG 1 cut(s) 945
Bst2UI CCWGG 2 cut(s) 1434, 1488
Bst4CI ACNGT 4 cut(s) 470, 510, 583, 1203
Bst6I CTCTTC 3 cut(s) 116, 405, 1187
BstC8I GCNNGC 3 cut(s) 7, 517, 1483
BstDEI CTNAG 3 cut(s) 404, 692, 1044
BstEII GGTNACC 1 cut(s) 1275
BstF5I GGATG 2 cut(s) 1309, 1454
BstKTI GATC 2 cut(s) 333, 791
BstMBI GATC 2 cut(s) 330, 788
BstMCI CGRYCG 1 cut(s) 361
BstMWI GCNNNNNNNGC 5 cut(s) 29, 98, 317, 836, 1295
BstNI CCWGG 2 cut(s) 1434, 1488
BstNSI RCATGY 2 cut(s) 1052, 1485
BstPI GGTNACC 1 cut(s) 1275
BstSCI CCNGG 6 cut(s) 114, 115, 1432, 1486, 1523, 1524
BstSFI CTRYAG 1 cut(s) 215
BstSLI GKGCMC 1 cut(s) 681
BstV1I GCAGC 7 cut(s) 304, 463, 818, 826, 1225, 1247, 1282
BstV2I GAAGAC 1 cut(s) 646
BstX2I RGATCY 1 cut(s) 788
BstYI RGATCY 1 cut(s) 788
Bsu15I ATCGAT 1 cut(s) 613
BsuRI GGCC 1 cut(s) 1438
BsuTUI ATCGAT 1 cut(s) 613
BtsCI GGATG 2 cut(s) 1309, 1454
BtsI GCAGTG 2 cut(s) 196, 354
BtsIMutI CAGTG 8 cut(s) 196, 354, 466, 515, 588, 942, 1199, 1213
BveI ACCTGC 1 cut(s) 208
Cac8I GCNNGC 3 cut(s) 7, 517, 1483
CciI TCATGA 1 cut(s) 235
Cfr13I GGNCC 2 cut(s) 956, 1436
Cfr9I CCCGGG 2 cut(s) 115, 1524
ClaI ATCGAT 1 cut(s) 613
CseI GACGC 1 cut(s) 64
Csp6I GTAC 1 cut(s) 1491
CviAII CATG 7 cut(s) 236, 385, 520, 758, 887, 1049, 1482
CviQI GTAC 1 cut(s) 1491
DdeI CTNAG 3 cut(s) 404, 692, 1044
DpnI GATC 2 cut(s) 332, 790
DpnII GATC 2 cut(s) 330, 788
DraIII CACNNNGTG 1 cut(s) 512
Eam1104I CTCTTC 3 cut(s) 116, 405, 1187
EarI CTCTTC 3 cut(s) 116, 405, 1187
Ecl136II GAGCTC 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 945
Eco24I GRGCYC 2 cut(s) 400, 1199
Eco47I GGWCC 1 cut(s) 956
Eco53kI GAGCTC 1 cut(s) 398
Eco88I CYCGRG 4 cut(s) 115, 269, 500, 1524
Eco91I GGTNACC 1 cut(s) 1275
EcoICRI GAGCTC 1 cut(s) 398
EcoO109I RGGNCCY 1 cut(s) 956
EcoO65I GGTNACC 1 cut(s) 1275
EcoRII CCWGG 2 cut(s) 1432, 1486
EcoT14I CCWWGG 1 cut(s) 945
EcoT38I GRGCYC 2 cut(s) 400, 1199
ErhI CCWWGG 1 cut(s) 945
FaeI CATG 7 cut(s) 239, 388, 523, 761, 890, 1052, 1485
FalI AAGNNNNNCTT 2 cut(s) 1327, 1359
FatI CATG 7 cut(s) 235, 384, 519, 757, 886, 1048, 1481
FauI CCCGC 1 cut(s) 823
Fnu4HI GCNGC 7 cut(s) 318, 477, 807, 840, 1236, 1239, 1296
FokI GGATG 2 cut(s) 1316, 1441
FriOI GRGCYC 2 cut(s) 400, 1199
Fsp4HI GCNGC 7 cut(s) 318, 477, 807, 840, 1236, 1239, 1296
FspBI CTAG 4 cut(s) 182, 516, 683, 1032
GluI GCNGC 7 cut(s) 318, 477, 807, 840, 1236, 1239, 1296
GsaI CCCAGC 1 cut(s) 1365
HaeIII GGCC 1 cut(s) 1438
HapII CCGG 2 cut(s) 116, 1525
HgaI GACGC 1 cut(s) 64
Hin1II CATG 7 cut(s) 239, 388, 523, 761, 890, 1052, 1485
HindIII AAGCTT 1 cut(s) 1336
HinfI GANTC 7 cut(s) 211, 503, 602, 733, 950, 998, 1040
HpaII CCGG 2 cut(s) 116, 1525
HphI GGTGA 2 cut(s) 1091, 1269
Hpy166II GTNNAC 2 cut(s) 679, 1171
Hpy188I TCNGA 7 cut(s) 263, 405, 492, 658, 972, 1045, 1081
Hpy8I GTNNAC 2 cut(s) 679, 1171
HpyAV CCTTC 4 cut(s) 91, 898, 1086, 1312
HpyCH4III ACNGT 4 cut(s) 470, 510, 583, 1203
HpyCH4IV ACGT 1 cut(s) 622
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 98, 317, 836, 1295
HpyF3I CTNAG 3 cut(s) 404, 692, 1044
HpySE526I ACGT 1 cut(s) 622
Hsp92II CATG 7 cut(s) 239, 388, 523, 761, 890, 1052, 1485
Kzo9I GATC 2 cut(s) 330, 788
LguI GCTCTTC 2 cut(s) 405, 1187
LmnI GCTCC 2 cut(s) 77, 854
Lsp1109I GCAGC 7 cut(s) 304, 463, 818, 826, 1225, 1247, 1282
LweI GCATC 3 cut(s) 463, 1282, 1463
MaeI CTAG 4 cut(s) 182, 516, 683, 1032
MaeII ACGT 1 cut(s) 622
MaeIII GTNAC 6 cut(s) 276, 504, 618, 781, 1275, 1426
MalI GATC 2 cut(s) 332, 790
MboI GATC 2 cut(s) 330, 788
MboII GAAGA 9 cut(s) 97, 103, 392, 651, 1104, 1138, 1204, 1327, 1361
MfeI CAATTG 1 cut(s) 993
MflI RGATCY 1 cut(s) 788
MhlI GDGCHC 3 cut(s) 400, 681, 1199
MluCI AATT 3 cut(s) 137, 257, 993
MlyI GAGTC 3 cut(s) 512, 596, 959
MmeI TCCRAC 1 cut(s) 985
MroXI GAANNNNTTC 1 cut(s) 257
MseI TTAA 5 cut(s) 282, 324, 441, 1263, 1532
MslI CAYNNNNRTG 2 cut(s) 471, 1475
MspA1I CMGCKG 2 cut(s) 170, 1238
MspI CCGG 2 cut(s) 116, 1525
MspR9I CCNGG 6 cut(s) 116, 117, 1434, 1488, 1525, 1526
MunI CAATTG 1 cut(s) 993
Mva1269I GAATGC 1 cut(s) 1237
MvaI CCWGG 2 cut(s) 1434, 1488
MwoI GCNNNNNNNGC 5 cut(s) 29, 98, 317, 836, 1295
NciI CCSGG 4 cut(s) 116, 117, 1525, 1526
NdeII GATC 2 cut(s) 330, 788
NheI GCTAGC 1 cut(s) 515
NlaIII CATG 7 cut(s) 239, 388, 523, 761, 890, 1052, 1485
NlaIV GGNNCC 2 cut(s) 698, 1386
NmeAIII GCCGAG 1 cut(s) 584
NmuCI GTSAC 3 cut(s) 504, 1275, 1426
NspI RCATGY 2 cut(s) 1052, 1485
PaeI GCATGC 1 cut(s) 1485
PagI TCATGA 1 cut(s) 235
PciSI GCTCTTC 2 cut(s) 405, 1187
PcsI WCGNNNNNNNCGW 1 cut(s) 619
PctI GAATGC 1 cut(s) 1237
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 4 cut(s) 211, 733, 998, 1040
PkrI GCNGC 7 cut(s) 319, 478, 808, 841, 1237, 1240, 1297
PleI GAGTC 3 cut(s) 511, 596, 958
PpsI GAGTC 3 cut(s) 511, 596, 958
PpuMI RGGWCCY 1 cut(s) 956
Psp124BI GAGCTC 1 cut(s) 400
Psp5II RGGWCCY 1 cut(s) 956
Psp6I CCWGG 2 cut(s) 1432, 1486
PspEI GGTNACC 1 cut(s) 1275
PspFI CCCAGC 1 cut(s) 1361
PspGI CCWGG 2 cut(s) 1432, 1486
PspN4I GGNNCC 2 cut(s) 698, 1386
PspPI GGNCC 2 cut(s) 956, 1436
PspPPI RGGWCCY 1 cut(s) 956
PsrI GAACNNNNNNTAC 4 cut(s) 141, 173, 1121, 1153
PstI CTGCAG 1 cut(s) 219
PsuI RGATCY 1 cut(s) 788
PvuII CAGCTG 2 cut(s) 170, 1238
RsaI GTAC 1 cut(s) 1492
RsaNI GTAC 1 cut(s) 1491
RseI CAYNNNNRTG 2 cut(s) 471, 1475
SacI GAGCTC 1 cut(s) 400
SapI GCTCTTC 2 cut(s) 405, 1187
SaqAI TTAA 5 cut(s) 282, 324, 441, 1263, 1532
SatI GCNGC 7 cut(s) 318, 477, 807, 840, 1236, 1239, 1296
Sau3AI GATC 2 cut(s) 330, 788
Sau96I GGNCC 2 cut(s) 956, 1436
SchI GAGTC 3 cut(s) 512, 596, 959
ScrFI CCNGG 6 cut(s) 116, 117, 1434, 1488, 1525, 1526
SduI GDGCHC 3 cut(s) 400, 681, 1199
SfaNI GCATC 3 cut(s) 463, 1282, 1463
SfcI CTRYAG 1 cut(s) 215
SinI GGWCC 1 cut(s) 956
SmaI CCCGGG 2 cut(s) 117, 1526
SmiMI CAYNNNNRTG 2 cut(s) 471, 1475
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
SphI GCATGC 1 cut(s) 1485
Sse9I AATT 3 cut(s) 137, 257, 993
SsiI CCGC 2 cut(s) 311, 830
SspI AATATT 3 cut(s) 130, 496, 1267
SspMI CTAG 4 cut(s) 182, 516, 683, 1032
SstI GAGCTC 1 cut(s) 400
StyD4I CCNGG 6 cut(s) 114, 115, 1432, 1486, 1523, 1524
StyI CCWWGG 1 cut(s) 945
TaaI ACNGT 4 cut(s) 470, 510, 583, 1203
TaiI ACGT 1 cut(s) 625
TaqI TCGA 3 cut(s) 613, 637, 1115
TaqII GACCGA 1 cut(s) 375
TasI AATT 3 cut(s) 137, 257, 993
TfiI GAWTC 4 cut(s) 211, 733, 998, 1040
Tru1I TTAA 5 cut(s) 282, 324, 441, 1263, 1532
Tru9I TTAA 5 cut(s) 282, 324, 441, 1263, 1532
TscAI CASTG 8 cut(s) 196, 354, 473, 515, 588, 942, 1206, 1213
TseFI GTSAC 3 cut(s) 504, 1275, 1426
TseI GCWGC 7 cut(s) 317, 476, 806, 839, 1235, 1238, 1295
Tsp45I GTSAC 3 cut(s) 504, 1275, 1426
TspDTI ATGAA 7 cut(s) 149, 401, 459, 536, 540, 1133, 1419
TspMI CCCGGG 2 cut(s) 115, 1524
TspRI CASTG 8 cut(s) 196, 354, 473, 515, 588, 942, 1206, 1213
VneI GTGCAC 1 cut(s) 677
VpaK11BI GGWCC 1 cut(s) 956
XceI RCATGY 2 cut(s) 1052, 1485
XmaI CCCGGG 2 cut(s) 115, 1524
XmnI GAANNNNTTC 1 cut(s) 257
XspI CTAG 4 cut(s) 182, 516, 683, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.