Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0008549

Physical Location & Seq

Genomic Coordinates Reverse (-)

26760622 .. 26765798

Gene Structure 5177 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1186100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2742 bp

ATGGGCGATCTTCGGGATTGGTCGCCTGAACCAAACGGCGCCGTGTTAGACGAGAGGGAGAGGGCTTCTTCGTCATCGTCGTTGTCGTCATCACCTCCTTCCCAATTTTCATCGAATCAACAGGCGGCGGCGGCTGCTTCTGCTTCTGCGCCGACGGCTATTAGCGCTGAGTACTGGCTGAGGGCGGAGGAGGCGACGCAGGGGGTCATAGCGCAGGTGCAGCCCACTGACGTGTCCGAGAGGAGGAGGAAGGCGGTCATCGATTACGCTCAGAGGCTCATCAAAAGTTGTCTTGGTTGCGAGGTGTTCCCATTTGGGTCTGTACCTCTAAAGACCTATCTGCCTGATGGAGATATTGATTTGACTGCTTTTGGTGGTATGAATGTTGAGGAGGCGCTGGCCAATGACGTCTGTGCTGTCCTTGAAAGGGAAGATCAAAATGTGGCTGCTGAGTTTATGGTGAAAGATGTCCAGTTGATTCGGGCAGAGGTCAAGCTTGTAAAGTGCCTTGTGCAAAATATTGTGGTCGATATTTCCTTCAATCAGTTAGGAGGGCTATGCACACTATGCTTCCTTGAGCAGGTTGATCGCCTCATTAGCAAAGACCATCTTTTTAAACGCAGTATCATACTAATTAAGGCCTGGTGCTATTATGAGAGCCGGATTCTTGGTGCCCATCATGGTTTGATTTCGACATATGCTTTGGAGACTTTGGTTCTGTATATTTTCCACCTCTTCCATTCATCTTTGAATGGTCCTCTAGCGGTCCTATATAAATTCTTGGACTACTTTAGTAAATTTGATTGGGACAGCTATTGTATCAGCTTGAGTGGTCCTGTTCGTTTATCTTCTCTTCCAGAACTCTTGGCTGAGACGCCAGAAAATGGTGGCGGTGATCTATTGCTGAGTAATGATTTTCTCAAGGGATGTGTGGAAAGGTTCTCAGTTCCTTCAAGAGGATATGAGACAAACCATAGAACATTTCAACCAAAGCATCTTAACATAGTTGATCCGCTTAAAGACAATAACAACCTTGGCCGAAGTGTGAGTAAAGGAAACTTTTACCGAATAAGAAGTGCATTCACATATGGTGCTCGAAAACTGGGGCGCATTCTTTCTCAGCCAGAAGTAAACATAGATGATGAGATCCGAAAGTTTTTCGCAAACACGTTGGACAGACATGGAGGCAGACAAAGGCCTGATGTCCAAGATCCTGCCCCATGGTCCATAAATGATGGGTATGGTAATGTGCCGTTATCGGTAGGTAGAGAGTCACAAGATCAGACACTTTATGAATCAGAATCTGCCTATTCTAGTGGCATGATTGGGGAATGTGGGTTAAATCATGAGAGATCAGGGAATGGTGAAGTTACAAGTGCTCATATATCTGGTCAAGGTCTGACTAGACCACATGAAACTGATATGAAACCTGATCAAGATGTTAATGGACCACATGAAAGCAGTATGAAGGTAGCATCCCCAACAATGTTATTGGAAGAGGACAGTTCCCCATATGGAATTGCTCTTTCCGAAAAGCGTCTTACAGGGGATGCAAAAGACCTTGCTACCTCCAGATTTCAAGGTCTTACAATTTCAAATGATGCTCAAAATCCTTTGCCGTCCAGTTGTGAAGTGAGTATATCTCCTTTAGGTAAAGTACAGCATGCACCTCATCTCTGGTCCTCTCACTCATCAACTGAAGATGGGGACATTAGCAATGGAAATCAAGATCAGCAATTGCCAGAAAGCTTTTGTTCAGCTGATAATTTGGTCGGAAACCAGGATGAGTATCAGCTGGGTTGTATTCAGGAAGCTCCATCTCCTGTTGGATCTAAGCACCATCCGTCACGGTTGAGTTCTGTTGTGTGTTCCTCCGATGACTTCTATCCTAGTTATTATCCAATGTCAAGTGGTATCGCTGGAAGTCCTAAACCTTCTAACTGCTTAACAGATCTGAGTGGGGATTACAATAGTCATTTACTTAGTCTAAACTATGGGCGTTGGTGTTACGACTGTGAGCTAAATGTGGCAATTCCTCCCATTGCGCCACCACCGGTGCACCCACAGTTTCAGGGCAAGAAGCCATGGGATGCAGTTCGAGAGTCGGTGCAGCACAGGCAGAAAGCATTTTCCCAGATGAATGTCAATGGTGTTGTCCCTAGGCCACCATTCTATCCAATGAACCCACCAATGTTGCCTAATGGCATAACCTTTGGTATGGAGGAGTTGCCAAAGCCACGTGGAACCGGAACGTACTTTCCCAATACGAACCATTTCAAAGATAGGCCTGTGACGCCAAGGGGAAGAAATCAAGCACCGCTGAGGTCTCATCGTAACAATGGCCATGCCATGATACCATCTTCAGAGAATTATACGTCCGACAGAAGCAGTCGTGATCTGTCACAACTACAAATGTCTCTTCATAAAGGTGGGGGAAAATCTGGATCTTCGGATAGCCCCGCTGGTTCCCCTAGAAGGAAAGTACACCCTAATGCGAATGGTTCAATTCATTCCTCTGAGAAAGTTGTAGAATTTGGGGCAATGGGACATGGCCCACCAGAGGGACCTGCAAATGGCAAACATACAGACGCTGGCTCATCATTCGGCCAGAACTCAAGCAGTGACCATTCATCACCAAGGATGCAGTTGACAAAAGCTGAGTTGGTTACAGATCAGGATAGGATCGCTGCACAGTCATACCGTTTGAAAGATGAAGAAGATTTCCCTCCTTTATCGGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

914

Amino Acids

100.04

Weight (kDa)

5.74

Isoelectric Point (pI)

48.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MTPAP-like_central	PF22600	67 - 186	1.4e-07	Poly(A) RNA polymerase, mitochondrial-like, central palm domain
PAP-OAS1	PF26180	199 - 391	3.1e-99	PAP/OAS1 substrate-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008549)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51620 AT3G51620 AT3G51620
fragaria_vesca	FvH4_6g10110 FvH4_6g10110 FvH4_6g10110 FvH4_6g10110 FvH4_6g10110
malus_domestica	MD04G1172800.v1.1 MD12G1186100.v1.1
prunus_persica	Prupe.6G291600_v2.0.a1
pyrus_communis	pycom04g15270 pycom12g17470
rosa_chinensis	RchiOBHm_Chr3g0460811
rosa_laevigata	RLG00000024979
rosa_multiflora	Rmu_sc0007678.1_g000014
rosa_roxburghii	Rroxscaffold_6G00419200
rosa_rugosa	Rorug03G0046500
rosa_samantha	Rh3AG103600 Rh3BG107300 Rh3CG109200 Rh3DG109100
rosa_wichuraiana	Rw3G008820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	205
AatII	GACGTC	1 cut(s)	411
Acc36I	ACCTGC	3 cut(s)	205, 571, 2575
AccB1I	GGYRCC	2 cut(s)	38, 671
AccB7I	CCANNNNNTGG	1 cut(s)	884
AclWI	GGATC	6 cut(s)	1004, 1141, 1205, 1837, 2452, 2690
AcoI	YGGCCR	4 cut(s)	399, 1036, 2341, 2605
AcsI	RAATTY	3 cut(s)	776, 797, 2531
AcuI	CTGAAG	2 cut(s)	1719, 2346
AcvI	CACGTG	1 cut(s)	2240
AcyI	GRCGYC	4 cut(s)	39, 408, 875, 2294
AfaI	GTAC	5 cut(s)	173, 324, 1659, 2255, 2484
AfeI	AGCGCT	1 cut(s)	166
AfiI	CCNNNNNNNGG	9 cut(s)	243, 427, 580, 884, 956, 2475, 2560, 2561, 2573
AflIII	ACRYGT	2 cut(s)	231, 1167
AgeI	ACCGGT	1 cut(s)	2053
AjiI	CACGTC	1 cut(s)	232
AjnI	CCWGG	2 cut(s)	641, 1779
AleI	CACNNNNGTG	1 cut(s)	230
AloI	GAACNNNNNNTCC	2 cut(s)	2242, 2274
AluBI	AGCT	9 cut(s)	496, 813, 825, 1749, 1760, 1795, 1814, 2020, 2657
AluI	AGCT	9 cut(s)	496, 813, 825, 1749, 1760, 1795, 1814, 2020, 2657
Alw21I	GWGCWC	3 cut(s)	1096, 1381, 2061
Alw26I	GTCTC	5 cut(s)	701, 866, 959, 2331, 2421
Alw44I	GTGCAC	1 cut(s)	2057
AlwI	GGATC	6 cut(s)	1004, 1141, 1205, 1837, 2452, 2690
AlwNI	CAGNNNCTG	2 cut(s)	1304, 2591
Aor51HI	AGCGCT	1 cut(s)	166
ApaLI	GTGCAC	1 cut(s)	2057
ApeKI	GCWGC	5 cut(s)	134, 220, 446, 2110, 2687
ApoI	RAATTY	3 cut(s)	776, 797, 2531
ArsI	GACNNNNNNTTYG	2 cut(s)	685, 717
AsiGI	ACCGGT	1 cut(s)	2053
Asp700I	GAANNNNTTC	1 cut(s)	2273
AspA2I	CCTAGG	1 cut(s)	2159
AspLEI	GCGC	7 cut(s)	41, 151, 167, 214, 397, 1110, 2047
AspS9I	GGNCC	8 cut(s)	755, 766, 833, 1224, 1448, 1680, 2552, 2564
AsuHPI	GGTGA	5 cut(s)	84, 472, 905, 1376, 2625
AvaII	GGWCC	7 cut(s)	755, 766, 833, 1224, 1448, 1680, 2564
AvrII	CCTAGG	1 cut(s)	2159
BaeGI	GKGCMC	2 cut(s)	676, 2061
BalI	TGGCCA	2 cut(s)	401, 2343
BanI	GGYRCC	2 cut(s)	38, 671
BbrPI	CACGTG	1 cut(s)	2240
Bbv12I	GWGCWC	3 cut(s)	1096, 1381, 2061
BbvCI	CCTCAGC	2 cut(s)	179, 2321
BbvI	GCAGC	5 cut(s)	121, 232, 433, 2122, 2674
BccI	CCATC	8 cut(s)	341, 615, 684, 1229, 1697, 1825, 1848, 2365
BceAI	ACGGC	5 cut(s)	26, 52, 171, 1237, 1603
BcgI	CGANNNNNNTGC	2 cut(s)	569, 603
BciT130I	CCWGG	2 cut(s)	643, 1781
BclI	TGATCA	1 cut(s)	1432
BcoDI	GTCTC	5 cut(s)	701, 866, 959, 2331, 2421
BfaI	CTAG	6 cut(s)	761, 1314, 1404, 1890, 2160, 2472
BfoI	RGCGCY	3 cut(s)	42, 168, 398
BfuAI	ACCTGC	3 cut(s)	205, 571, 2575
BglII	AGATCT	1 cut(s)	1951
BisI	GCNGC	8 cut(s)	126, 129, 132, 135, 221, 447, 2111, 2688
BlnI	CCTAGG	1 cut(s)	2159
BlsI	GCNGC	8 cut(s)	127, 130, 133, 136, 222, 448, 2112, 2689
BmcAI	AGTACT	1 cut(s)	173
Bme1390I	CCNGG	2 cut(s)	643, 1781
Bme18I	GGWCC	7 cut(s)	755, 766, 833, 1224, 1448, 1680, 2564
BmgBI	CACGTC	1 cut(s)	232
BmgT120I	GGNCC	8 cut(s)	755, 766, 833, 1224, 1448, 1680, 2552, 2564
BmiI	GGNNCC	5 cut(s)	40, 673, 2245, 2467, 2565
BmrFI	CCNGG	2 cut(s)	643, 1781
BmrI	ACTGGG	1 cut(s)	1112
BmsI	GCATC	6 cut(s)	1003, 1484, 1540, 1591, 2080, 2631
BmuI	ACTGGG	1 cut(s)	1112
BplI	GAGNNNNNCTC	2 cut(s)	1627, 1659
BpmI	CTGGAG	1 cut(s)	1555
Bpu10I	CCTNAGC	2 cut(s)	179, 2321
BpuEI	CTTGAG	4 cut(s)	596, 847, 905, 2599
Bsa29I	ATCGAT	1 cut(s)	261
BsaAI	YACGTR	1 cut(s)	2240
BsaBI	GATNNNNATC	1 cut(s)	1788
BsaHI	GRCGYC	4 cut(s)	39, 408, 875, 2294
BsaI	GGTCTC	1 cut(s)	2331
BsaJI	CCNNGG	6 cut(s)	1033, 1220, 2084, 2159, 2297, 2636
BsaWI	WCCGGW	2 cut(s)	2053, 2246
Bsc4I	CCNNNNNNNGG	9 cut(s)	243, 427, 580, 884, 956, 2475, 2560, 2561, 2573
Bse118I	RCCGGY	1 cut(s)	2053
Bse1I	ACTGG	4 cut(s)	179, 472, 1107, 1623
Bse3DI	GCAATG	3 cut(s)	1723, 2040, 2547
Bse8I	GATNNNNATC	1 cut(s)	1788
BseBI	CCWGG	2 cut(s)	643, 1781
BseCI	ATCGAT	1 cut(s)	261
BseDI	CCNNGG	6 cut(s)	1033, 1220, 2084, 2159, 2297, 2636
BseGI	GGATG	7 cut(s)	932, 1475, 1555, 1789, 1840, 2095, 2646
BseJI	GATNNNNATC	1 cut(s)	1788
BseLI	CCNNNNNNNGG	9 cut(s)	243, 427, 580, 884, 956, 2475, 2560, 2561, 2573
BseMI	GCAATG	3 cut(s)	1723, 2040, 2547
BseNI	ACTGG	4 cut(s)	179, 472, 1107, 1623
BseRI	GAGGAG	5 cut(s)	203, 256, 259, 404, 2237
BseSI	GKGCMC	2 cut(s)	676, 2061
BseXI	GCAGC	5 cut(s)	121, 232, 433, 2122, 2674
BseYI	CCCAGC	1 cut(s)	1795
BsgI	GTGCAG	3 cut(s)	239, 2129, 2673
BshNI	GGYRCC	2 cut(s)	38, 671
BshTI	ACCGGT	1 cut(s)	2053
BshVI	ATCGAT	1 cut(s)	261
BsiHKAI	GWGCWC	3 cut(s)	1096, 1381, 2061
BsiSI	CCGG	3 cut(s)	661, 2054, 2247
BslFI	GGGAC	5 cut(s)	821, 1721, 2141, 2559, 2577
BslI	CCNNNNNNNGG	9 cut(s)	243, 427, 580, 884, 956, 2475, 2560, 2561, 2573
BsmAI	GTCTC	5 cut(s)	701, 866, 959, 2331, 2421
BsmBI	CGTCTC	1 cut(s)	866
BsmFI	GGGAC	5 cut(s)	821, 1721, 2141, 2559, 2577
BsmI	GAATGC	2 cut(s)	1079, 1110
Bso31I	GGTCTC	1 cut(s)	2331
Bsp1286I	GDGCHC	4 cut(s)	676, 1096, 1381, 2061
Bsp19I	CCATGG	2 cut(s)	1220, 2084
BspDI	ATCGAT	1 cut(s)	261
BspHI	TCATGA	1 cut(s)	1345
BspLI	GGNNCC	5 cut(s)	40, 673, 2245, 2467, 2565
BspMI	ACCTGC	3 cut(s)	205, 571, 2575
BspPI	GGATC	6 cut(s)	1004, 1141, 1205, 1837, 2452, 2690
BspT107I	GGYRCC	2 cut(s)	38, 671
BspTNI	GGTCTC	1 cut(s)	2331
BsrDI	GCAATG	3 cut(s)	1723, 2040, 2547
BsrFI	RCCGGY	1 cut(s)	2053
BsrI	ACTGG	4 cut(s)	179, 472, 1107, 1623
BssAI	RCCGGY	1 cut(s)	2053
BssECI	CCNNGG	6 cut(s)	1033, 1220, 2084, 2159, 2297, 2636
BssNI	GRCGYC	4 cut(s)	39, 408, 875, 2294
BssT1I	CCWWGG	6 cut(s)	1033, 1220, 2084, 2159, 2297, 2636
Bst2UI	CCWGG	2 cut(s)	643, 1781
Bst4CI	ACNGT	6 cut(s)	1505, 1851, 2015, 2067, 2694, 2702
Bst6I	CTCTTC	4 cut(s)	740, 858, 1491, 2424
BstACI	GRCGYC	4 cut(s)	39, 408, 875, 2294
BstAPI	GCANNNNNTGC	1 cut(s)	567
BstBAI	YACGTR	1 cut(s)	2240
BstC8I	GCNNGC	3 cut(s)	399, 1665, 2593
BstDSI	CCRYGG	2 cut(s)	1220, 2084
BstENI	CCTNNNNNAGG	2 cut(s)	578, 954
BstF5I	GGATG	7 cut(s)	932, 1475, 1555, 1789, 1840, 2095, 2646
BstH2I	RGCGCY	3 cut(s)	42, 168, 398
BstHHI	GCGC	7 cut(s)	41, 151, 167, 214, 397, 1110, 2047
BstMAI	GTCTC	5 cut(s)	701, 866, 959, 2331, 2421
BstNI	CCWGG	2 cut(s)	643, 1781
BstNSI	RCATGY	1 cut(s)	1667
BstSCI	CCNGG	2 cut(s)	641, 1779
BstSLI	GKGCMC	2 cut(s)	676, 2061
BstV1I	GCAGC	5 cut(s)	121, 232, 433, 2122, 2674
BstX2I	RGATCY	5 cut(s)	1146, 1210, 1829, 1951, 2444
BstYI	RGATCY	5 cut(s)	1146, 1210, 1829, 1951, 2444
Bsu15I	ATCGAT	1 cut(s)	261
BsuTUI	ATCGAT	1 cut(s)	261
BtgI	CCRYGG	2 cut(s)	1220, 2084
BtrI	CACGTC	1 cut(s)	232
BtsCI	GGATG	7 cut(s)	932, 1475, 1555, 1789, 1840, 2095, 2646
BtsI	GCAGTG	1 cut(s)	2626
BtsIMutI	CAGTG	2 cut(s)	225, 2626
BveI	ACCTGC	3 cut(s)	205, 571, 2575
Cac8I	GCNNGC	3 cut(s)	399, 1665, 2593
CaiI	CAGNNNCTG	2 cut(s)	1304, 2591
CciI	TCATGA	1 cut(s)	1345
CfoI	GCGC	7 cut(s)	41, 151, 167, 214, 397, 1110, 2047
Cfr10I	RCCGGY	1 cut(s)	2053
Cfr13I	GGNCC	8 cut(s)	755, 766, 833, 1224, 1448, 1680, 2552, 2564
ClaI	ATCGAT	1 cut(s)	261
CseI	GACGC	5 cut(s)	205, 883, 1526, 2302, 2597
Csp6I	GTAC	5 cut(s)	172, 323, 1658, 2254, 2483
CspAI	ACCGGT	1 cut(s)	2053
CspCI	CAANNNNNGTGG	2 cut(s)	2175, 2210
CviQI	GTAC	5 cut(s)	172, 323, 1658, 2254, 2483
DinI	GGCGCC	1 cut(s)	40
DraI	TTTAAA	1 cut(s)	616
EaeI	YGGCCR	4 cut(s)	399, 1036, 2341, 2605
Eam1104I	CTCTTC	4 cut(s)	740, 858, 1491, 2424
EarI	CTCTTC	4 cut(s)	740, 858, 1491, 2424
EciI	GGCGGA	1 cut(s)	200
Eco130I	CCWWGG	6 cut(s)	1033, 1220, 2084, 2159, 2297, 2636
Eco147I	AGGCCT	3 cut(s)	641, 1198, 2287
Eco31I	GGTCTC	1 cut(s)	2331
Eco47I	GGWCC	7 cut(s)	755, 766, 833, 1224, 1448, 1680, 2564
Eco47III	AGCGCT	1 cut(s)	166
Eco57I	CTGAAG	2 cut(s)	1719, 2346
Eco72I	CACGTG	1 cut(s)	2240
EcoNI	CCTNNNNNAGG	2 cut(s)	578, 954
EcoO109I	RGGNCCY	1 cut(s)	2564
EcoRII	CCWGG	2 cut(s)	641, 1779
EcoT14I	CCWWGG	6 cut(s)	1033, 1220, 2084, 2159, 2297, 2636
EgeI	GGCGCC	1 cut(s)	40
EheI	GGCGCC	1 cut(s)	40
ErhI	CCWWGG	6 cut(s)	1033, 1220, 2084, 2159, 2297, 2636
Esp3I	CGTCTC	1 cut(s)	866
FalI	AAGNNNNNCTT	2 cut(s)	594, 626
FaqI	GGGAC	5 cut(s)	821, 1721, 2141, 2559, 2577
FauI	CCCGC	1 cut(s)	2467
FauNDI	CATATG	3 cut(s)	697, 1087, 1513
FbaI	TGATCA	1 cut(s)	1432
Fnu4HI	GCNGC	8 cut(s)	126, 129, 132, 135, 221, 447, 2111, 2688
FokI	GGATG	7 cut(s)	939, 1462, 1562, 1796, 1827, 2102, 2653
Fsp4HI	GCNGC	8 cut(s)	126, 129, 132, 135, 221, 447, 2111, 2688
FspBI	CTAG	6 cut(s)	761, 1314, 1404, 1890, 2160, 2472
GlaI	GCGC	7 cut(s)	40, 150, 166, 213, 396, 1109, 2046
GluI	GCNGC	8 cut(s)	126, 129, 132, 135, 221, 447, 2111, 2688
GsaI	CCCAGC	1 cut(s)	1799
GsuI	CTGGAG	1 cut(s)	1555
HaeII	RGCGCY	3 cut(s)	42, 168, 398
HapII	CCGG	3 cut(s)	661, 2054, 2247
HgaI	GACGC	5 cut(s)	205, 883, 1526, 2302, 2597
HhaI	GCGC	7 cut(s)	41, 151, 167, 214, 397, 1110, 2047
Hin1I	GRCGYC	4 cut(s)	39, 408, 875, 2294
Hin6I	GCGC	7 cut(s)	39, 149, 165, 212, 395, 1108, 2045
HinP1I	GCGC	7 cut(s)	39, 149, 165, 212, 395, 1108, 2045
HincII	GTYRAC	1 cut(s)	2649
HindII	GTYRAC	1 cut(s)	2649
HindIII	AAGCTT	2 cut(s)	494, 1747
HinfI	GANTC	7 cut(s)	115, 478, 664, 1271, 1295, 1301, 2102
HpaII	CCGG	3 cut(s)	661, 2054, 2247
HphI	GGTGA	5 cut(s)	84, 472, 905, 1376, 2625
Hpy166II	GTNNAC	4 cut(s)	1132, 2059, 2485, 2649
Hpy8I	GTNNAC	4 cut(s)	1132, 2059, 2485, 2649
Hpy99I	CGWCG	3 cut(s)	82, 157, 199
HpyAV	CCTTC	7 cut(s)	108, 244, 547, 960, 1462, 1944, 2469
HpyCH4III	ACNGT	6 cut(s)	1505, 1851, 2015, 2067, 2694, 2702
HpyCH4IV	ACGT	6 cut(s)	231, 408, 1169, 2239, 2252, 2375
HpySE526I	ACGT	6 cut(s)	231, 408, 1169, 2239, 2252, 2375
Hsp92I	GRCGYC	4 cut(s)	39, 408, 875, 2294
HspAI	GCGC	7 cut(s)	39, 149, 165, 212, 395, 1108, 2045
KasI	GGCGCC	1 cut(s)	38
Ksp22I	TGATCA	1 cut(s)	1432
LmnI	GCTCC	1 cut(s)	1819
Lsp1109I	GCAGC	5 cut(s)	121, 232, 433, 2122, 2674
LweI	GCATC	6 cut(s)	1003, 1484, 1540, 1591, 2080, 2631
MaeI	CTAG	6 cut(s)	761, 1314, 1404, 1890, 2160, 2472
MaeII	ACGT	6 cut(s)	231, 408, 1169, 2239, 2252, 2375
MaeIII	GTNAC	9 cut(s)	1272, 1369, 1845, 2006, 2290, 2333, 2400, 2621, 2665
MfeI	CAATTG	1 cut(s)	1736
MflI	RGATCY	5 cut(s)	1146, 1210, 1829, 1951, 2444
MhlI	GDGCHC	4 cut(s)	676, 1096, 1381, 2061
MlsI	TGGCCA	2 cut(s)	401, 2343
MluNI	TGGCCA	2 cut(s)	401, 2343
Mly113I	GGCGCC	1 cut(s)	39
MlyI	GAGTC	2 cut(s)	1280, 2111
MmeI	TCCRAC	4 cut(s)	1152, 1753, 1807, 2403
Mox20I	TGGCCA	2 cut(s)	401, 2343
MroXI	GAANNNNTTC	1 cut(s)	2273
MscI	TGGCCA	2 cut(s)	401, 2343
MseI	TTAA	7 cut(s)	615, 636, 999, 1017, 1340, 1443, 1946
MslI	CAYNNNNRTG	3 cut(s)	230, 2427, 2490
Msp20I	TGGCCA	2 cut(s)	401, 2343
MspA1I	CMGCKG	4 cut(s)	1760, 1795, 2320, 2462
MspI	CCGG	3 cut(s)	661, 2054, 2247
MspR9I	CCNGG	2 cut(s)	643, 1781
MunI	CAATTG	1 cut(s)	1736
Mva1269I	GAATGC	2 cut(s)	1079, 1110
MvaI	CCWGG	2 cut(s)	643, 1781
NarI	GGCGCC	1 cut(s)	39
NcoI	CCATGG	2 cut(s)	1220, 2084
NdeI	CATATG	3 cut(s)	697, 1087, 1513
NlaIV	GGNNCC	5 cut(s)	40, 673, 2245, 2467, 2565
NmuCI	GTSAC	5 cut(s)	1272, 1845, 2290, 2400, 2621
NspI	RCATGY	1 cut(s)	1667
OliI	CACNNNNGTG	1 cut(s)	230
PaeI	GCATGC	1 cut(s)	1667
PagI	TCATGA	1 cut(s)	1345
PaqCI	CACCTGC	1 cut(s)	205
PceI	AGGCCT	3 cut(s)	641, 1198, 2287
PcsI	WCGNNNNNNNCGW	2 cut(s)	77, 83
PctI	GAATGC	2 cut(s)	1079, 1110
PdmI	GAANNNNTTC	1 cut(s)	2273
PfeI	GAWTC	5 cut(s)	115, 478, 664, 1295, 1301
PflMI	CCANNNNNTGG	1 cut(s)	884
PinAI	ACCGGT	1 cut(s)	2053
PkrI	GCNGC	8 cut(s)	127, 130, 133, 136, 222, 448, 2112, 2689
PleI	GAGTC	2 cut(s)	1279, 2110
PluTI	GGCGCC	1 cut(s)	42
PmaCI	CACGTG	1 cut(s)	2240
PmlI	CACGTG	1 cut(s)	2240
PpsI	GAGTC	2 cut(s)	1279, 2110
Ppu21I	YACGTR	1 cut(s)	2240
PpuMI	RGGWCCY	1 cut(s)	2564
Psp5II	RGGWCCY	1 cut(s)	2564
Psp6I	CCWGG	2 cut(s)	641, 1779
PspCI	CACGTG	1 cut(s)	2240
PspFI	CCCAGC	1 cut(s)	1795
PspGI	CCWGG	2 cut(s)	641, 1779
PspN4I	GGNNCC	5 cut(s)	40, 673, 2245, 2467, 2565
PspPI	GGNCC	8 cut(s)	755, 766, 833, 1224, 1448, 1680, 2552, 2564
PspPPI	RGGWCCY	1 cut(s)	2564
PstNI	CAGNNNCTG	2 cut(s)	1304, 2591
PsuI	RGATCY	5 cut(s)	1146, 1210, 1829, 1951, 2444
PvuII	CAGCTG	2 cut(s)	1760, 1795
RsaI	GTAC	5 cut(s)	173, 324, 1659, 2255, 2484
RsaNI	GTAC	5 cut(s)	172, 323, 1658, 2254, 2483
RseI	CAYNNNNRTG	3 cut(s)	230, 2427, 2490
SaqAI	TTAA	7 cut(s)	615, 636, 999, 1017, 1340, 1443, 1946
SatI	GCNGC	8 cut(s)	126, 129, 132, 135, 221, 447, 2111, 2688
Sau96I	GGNCC	8 cut(s)	755, 766, 833, 1224, 1448, 1680, 2552, 2564
ScaI	AGTACT	1 cut(s)	173
SchI	GAGTC	2 cut(s)	1280, 2111
ScrFI	CCNGG	2 cut(s)	643, 1781
SduI	GDGCHC	4 cut(s)	676, 1096, 1381, 2061
SfaNI	GCATC	6 cut(s)	1003, 1484, 1540, 1591, 2080, 2631
SfoI	GGCGCC	1 cut(s)	40
SgrAI	CRCCGGYG	1 cut(s)	2053
SinI	GGWCC	7 cut(s)	755, 766, 833, 1224, 1448, 1680, 2564
SmiMI	CAYNNNNRTG	3 cut(s)	230, 2427, 2490
SmlI	CTYRAG	4 cut(s)	575, 826, 920, 2614
SmoI	CTYRAG	4 cut(s)	575, 826, 920, 2614
SphI	GCATGC	1 cut(s)	1667
SseBI	AGGCCT	3 cut(s)	641, 1198, 2287
SspDI	GGCGCC	1 cut(s)	38
SspI	AATATT	1 cut(s)	520
SspMI	CTAG	6 cut(s)	761, 1314, 1404, 1890, 2160, 2472
StuI	AGGCCT	3 cut(s)	641, 1198, 2287
StyD4I	CCNGG	2 cut(s)	641, 1779
StyI	CCWWGG	6 cut(s)	1033, 1220, 2084, 2159, 2297, 2636
TaaI	ACNGT	6 cut(s)	1505, 1851, 2015, 2067, 2694, 2702
TaiI	ACGT	6 cut(s)	234, 411, 1172, 2242, 2255, 2378
TaqI	TCGA	6 cut(s)	113, 261, 528, 692, 1096, 2098
TatI	WGTACW	3 cut(s)	171, 1657, 2482
TauI	GCSGC	3 cut(s)	128, 131, 134
TfiI	GAWTC	5 cut(s)	115, 478, 664, 1295, 1301
Tru1I	TTAA	7 cut(s)	615, 636, 999, 1017, 1340, 1443, 1946
Tru9I	TTAA	7 cut(s)	615, 636, 999, 1017, 1340, 1443, 1946
TscAI	CASTG	2 cut(s)	232, 2626
TseFI	GTSAC	5 cut(s)	1272, 1845, 2290, 2400, 2621
TseI	GCWGC	5 cut(s)	134, 220, 446, 2110, 2687
Tsp45I	GTSAC	5 cut(s)	1272, 1845, 2290, 2400, 2621
TspGWI	ACGGA	1 cut(s)	1833
TspRI	CASTG	2 cut(s)	232, 2626
Van91I	CCANNNNNTGG	1 cut(s)	884
VneI	GTGCAC	1 cut(s)	2057
VpaK11BI	GGWCC	7 cut(s)	755, 766, 833, 1224, 1448, 1680, 2564
XagI	CCTNNNNNAGG	2 cut(s)	578, 954
XapI	RAATTY	3 cut(s)	776, 797, 2531
XceI	RCATGY	1 cut(s)	1667
XmaJI	CCTAGG	1 cut(s)	2159
XmnI	GAANNNNTTC	1 cut(s)	2273
XspI	CTAG	6 cut(s)	761, 1314, 1404, 1890, 2160, 2472
ZraI	GACGTC	1 cut(s)	409
ZrmI	AGTACT	1 cut(s)	173

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.